Genomic and Phenotypic Characterization of Pseudomonas juntendi Strain A3e, a Drug-Resistant Isolate from Irrigation Water.

IF 2.6 3区生物学 Q3 MICROBIOLOGY

Current Microbiology Pub Date : 2025-07-24 DOI:10.1007/s00284-025-04371-2

Ángel Ibarra-Rodríguez, Jean Pierre González-Gómez, Manuel Ángel Castillo-Olea, Bruno Gomez-Gil, Nohelia Castro-Del Campo, Cristobal Chaidez

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Abstract

Pseudomonas juntendi is a newly recognized opportunistic pathogen often exhibiting multi-drug resistance and commonly isolated from clinical settings. Here, we report the characterization of P. juntendi strain A3e, isolated from irrigation water, which was analyzed using antimicrobial susceptibility testing and whole-genome sequencing. Taxonomic classification via multi-locus sequence typing and average nucleotide identity analysis confirmed the strain's identity as P. juntendi with over 99% similarity. Phenotypic testing revealed strong biofilm formation and resistance to four antibiotics and intermediate resistance to two additional antibiotics. Genomic analysis identified 24 antibiotic-resistance genes, predominantly related to efflux pumps, and 50 virulence-related genes involved in adhesion, motility, biofilm formation, and siderophore uptake. This is the first genome of P. juntendi reported in Mexico; despite its environmental origin, the potential clinical implications warrant attention.

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灌溉水中耐药分离株juntendi假单胞菌A3e的基因组和表型特征

juntendi假单胞菌是一种新发现的条件致病菌，通常表现为多重耐药，通常从临床环境中分离出来。在这里，我们报道了从灌溉水中分离的P. juntendi菌株A3e的特性，并利用抗菌药敏试验和全基因组测序对其进行了分析。通过多位点序列分型和平均核苷酸同源性分析，证实该菌株为P. juntendi，相似度超过99%。表型检测显示生物膜形成较强，对四种抗生素耐药，对另外两种抗生素耐药。基因组分析确定了24个抗生素耐药基因，主要与外排泵有关，以及50个与粘附、运动、生物膜形成和铁载体摄取有关的毒力相关基因。这是在墨西哥报道的第一个juntendi疟原虫基因组；尽管其环境起源，潜在的临床意义值得关注。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current Microbiology 生物-微生物学

CiteScore

4.80

自引率

3.80%

发文量

380

审稿时长

2.5 months

期刊介绍： Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.