Current Microbiology最新文献_第3页

Proteomic Analysis Reveals Differentially Expressed Proteins in Cordyceps militaris Cultured with Different Media.

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-12-06 DOI: 10.1007/s00284-024-04005-z

Qiurong Jia, Jiahui Zhang, Shenghou Wang, Fangxu Xu

引用次数: 0

Lipopeptides from Bacillus atrophaeus MCM61 and melatonin suppress gray mold-induced oxidative stress in cut roses (Rosa hybrida L.) and improve vase life.

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-12-03 DOI: 10.1007/s00284-024-03987-0

Javaria Malik, Anam Moosa, Faisal Zulfiqar, Fayez Althobaiti, Hina Andaleeb, Nurah M Alzamel, Sultan F Alsharari, Eman Fayad, Fahad Al-Asmari, Özhan Şimşek, Tolga İzgü, Mehmet Tütüncü, Mohammed Ali Al-Duais, Madeha O I Ghobashy, Hayam A Alwabsi, Nahla Zidan, Mohamed Sakran, Tarek M A Soliman

{"title":"Lipopeptides from Bacillus atrophaeus MCM61 and melatonin suppress gray mold-induced oxidative stress in cut roses (Rosa hybrida L.) and improve vase life.","authors":"Javaria Malik, Anam Moosa, Faisal Zulfiqar, Fayez Althobaiti, Hina Andaleeb, Nurah M Alzamel, Sultan F Alsharari, Eman Fayad, Fahad Al-Asmari, Özhan Şimşek, Tolga İzgü, Mehmet Tütüncü, Mohammed Ali Al-Duais, Madeha O I Ghobashy, Hayam A Alwabsi, Nahla Zidan, Mohamed Sakran, Tarek M A Soliman","doi":"10.1007/s00284-024-03987-0","DOIUrl":"https://doi.org/10.1007/s00284-024-03987-0","url":null,"abstract":"Application of melatonin and lipopeptides (LPs) derived from Bacillus strains is considered an efficient strategy to control plant diseases at both pre and postharvest stages. However, the combined application of melatonin and LPs has not been studied yet. Therefore, the present study presents the synergistic effect of melatonin and LPs produced by Bacillus atrophaeus strain MCM61 against gray mold disease and its impact on quality parameters and vase life of cut roses. The stems of cut roses along with flowers were dipped in the melatonin solution at concentration (0, 0.2, 0.4, 0.6, and 0.8 mM) and the results indicated that the vase life of flowers treated with the melatonin was enhanced compared to control. Melatonin at 0.6 mM concentration showed the highest vase life of cut roses at day 8. Synergistic treatment with melatonin and LPs of B. atrophaeus MCM61 revealed that MDA and H2O2 content showed the highest decrease in cut roses. Similarly, relative water content, total phenol content, GSH content, and defense enzymes i.e., APX, SOD, PPO, POD, and CAT activities were increased in cut roses treated with co-application of B. atrophaeus MCM61 LPs and melatonin compared to single treatments and control treatment. Furthermore, the longevity of cut roses was also improved in flowers treated with a combined application of MCM61 and melatonin compared to other treatments.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 1","pages":"28"},"PeriodicalIF":2.3,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Topical Infection of Cordyceps militaris in Silkworm Larvae Through the Cuticle has Lower Infectivity Compared to Beauveria bassiana and Metarhizium anisopliae.

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-12-02 DOI: 10.1007/s00284-024-03989-y

Tatsuya Kato, Sota Inagaki, Chisato Shibata, Keito Takayanagi, Hiroki Uehara, Konomi Nishimura, Enoch Y Park

{"title":"Topical Infection of Cordyceps militaris in Silkworm Larvae Through the Cuticle has Lower Infectivity Compared to Beauveria bassiana and Metarhizium anisopliae.","authors":"Tatsuya Kato, Sota Inagaki, Chisato Shibata, Keito Takayanagi, Hiroki Uehara, Konomi Nishimura, Enoch Y Park","doi":"10.1007/s00284-024-03989-y","DOIUrl":"https://doi.org/10.1007/s00284-024-03989-y","url":null,"abstract":"Topical infection of entomopathogenic fungi in insects occurs when the fungal conidia attach to the insect's surface (cuticle), germinate, and then form appressoria that penetrate the cuticle and enter their bodies. In this study, we inoculated silkworm larvae with three entomopathogenic fungi, Cordyceps militaris, Beauveria bassiana, and Metarhizium anisopliae, and investigated their mechanisms of infection. Attachment of the conidia of the three entomopathogenic fungi to the surface of silkworm larvae was observed under a microscope. We counted the number of conidia attached to the surface of the silkworm larvae and the number of conidia detached from the surface was counted. The number of C. militaris conidia that attached to the surface was less than that of B. bassiana and M. anisopliae; however, it germinated and formed appressoria on hydrophobic surfaces, similar to the other two strains. Mycelial growth of C. militaris was inhibited compared to that of B. bassiana in PDA medium containing 0.1% linoleic and linolenic acids. The germination of C. militaris conidia was also inhibited in PD medium containing 0.1% linoleic or linolenic acids. These results suggest that the attachment of low numbers of C. militaris conidia on the surface of silkworm larvae and presence of inhibitory linoleic or linolenic acids in the silkworm cuticles may cause low topical infectivity by C. militaris. This study improves the efficacy of topically infecting silkworms with C. militaris to produce fungal fruiting bodies for use in traditional Chinese medicine and dietary supplement production.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 1","pages":"26"},"PeriodicalIF":2.3,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of a Putative Lysin from Enterococcus faecalis Phage IME-EFm1 and Determination of its Protective Efficacy in Mice.

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-12-02 DOI: 10.1007/s00284-024-03973-6

Can Wang, Youhong Guan, Wanrong Wang, Binbin Zhang, Pulin Li, Rui Han, Wenlong Wu, Sijing Zhou, Ran Wang

{"title":"Characterization of a Putative Lysin from Enterococcus faecalis Phage IME-EFm1 and Determination of its Protective Efficacy in Mice.","authors":"Can Wang, Youhong Guan, Wanrong Wang, Binbin Zhang, Pulin Li, Rui Han, Wenlong Wu, Sijing Zhou, Ran Wang","doi":"10.1007/s00284-024-03973-6","DOIUrl":"https://doi.org/10.1007/s00284-024-03973-6","url":null,"abstract":"The rising tide of bacterial drug resistance has sparked renewed interest in bacteriophages, the natural predators of bacteria. Our study highlights IME-EFm1, a Caudoviricetes bacteriophage specifically targeting Enterococcus faecium. Through our investigations, we identified that the gene IME-EFm1-ORF24 encodes an amidase, referred to as gp24, with promising lytic capabilities. Remarkably, gp24 exhibited a wider lytic spectrum than its parent phage, successfully lysing 26 out of 32 E. faecium strains, compared to the phage's ability to lyse only 21. This protein demonstrated robust antibacterial activity, remaining effective at temperatures between 25 °C and 60 °C and across a pH range of 6 to 12. Additionally, gp24 displayed significant anti-biofilm properties, effectively dismantling established biofilms in vitro. In a mouse model of abdominal infection, gp24 achieved a 75% protection rate against a dose of 2 × 109 colony-forming units of E. faecium En383, significantly outperforming the control group (p < 0.05). These compelling results suggest that gp24 holds great potential as a novel antimicrobial agent for treating E. faecium infections.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 1","pages":"27"},"PeriodicalIF":2.3,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Analysis of Eugenol-Loaded Ag-Co and Unloaded Ag-Co Bimetallic Nanoparticles Against Escherichia coli.

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-11-30 DOI: 10.1007/s00284-024-03983-4

Aarya Sahay, Rajesh Singh Tomar, Vikas Shrivastava, Suresh K Verma, Pallavi Singh Chauhan

{"title":"Comparative Analysis of Eugenol-Loaded Ag-Co and Unloaded Ag-Co Bimetallic Nanoparticles Against Escherichia coli.","authors":"Aarya Sahay, Rajesh Singh Tomar, Vikas Shrivastava, Suresh K Verma, Pallavi Singh Chauhan","doi":"10.1007/s00284-024-03983-4","DOIUrl":"https://doi.org/10.1007/s00284-024-03983-4","url":null,"abstract":"Recent developments in nanotechnology have revealed the significance of bimetallic nanoparticles for various applications. This study reveals the facile green synthesis of Ag-Co bimetallic nanoparticles using eugenol. A comparative analysis of Eugenol-loaded Ag-Co and unloaded Ag-Co bimetallic nanoparticle (BNPs) was done to investigate their antibacterial and antioxidant activity using flow cytometry. The biosynthesized Eugenol-loaded and unloaded BNPs were evaluated for antibacterial activity against Escherichia coli. The antioxidant activity was analyzed by using a DPPH scavenging activity assay and flow cytometry. UV-Vis spectroscopic analysis of synthesized eugenol-loaded and unloaded BNPs showed absorbance at 257 nm and 240 nm, respectively. FTIR analysis showed the peak range in the 500-4000 cm-1 corresponds to different functional groups. Zeta potential confirms the formation of stable and uniformly synthesized BNPs. EDX and SEM analysis confirm the elemental compositions and the size of the BNPs, respectively. Flow cytometric analysis revealed the live-dead parameters of Escherichia coli, when exposed to different concentrations of Eugenol-loaded Ag-Co (Eu@Ag-Co) and Unloaded Ag-Co BNPs. In addition, comparative MIC and MBC values of eugenol-loaded and unloaded BNPs were obtained for Escherichia coli. The Antioxidant activity revealed the comparative higher significant DPPH scavenging activity of Eu@Ag-Co. Moreover, the flow cytometric analysis confirmed the higher antibacterial efficacy of Eu@Ag-Co over unloaded Ag-Co BNPs by showing a higher percentage of dead cells. The study determined the enhanced antibacterial and antioxidant activity of nanoparticles by Eugenol loading and advocated it to be a better therapeutic approach. This study encourages the use of biosynthesized loaded-phytochemicals BNPs over unloaded BNPs.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 1","pages":"23"},"PeriodicalIF":2.3,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142754800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bile's Hidden Weapon: Modulating the Microbiome and Tumor Microenvironment.

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-11-30 DOI: 10.1007/s00284-024-04004-0

Mohamed J Saadh, Hanan Hassan Ahmed, Ali Fawzi Al-Hussainy, Irwanjot Kaur, Abhishek Kumar, Mamata Chahar, Suman Saini, Waam Mohammed Taher, Mariem Alwan, Mahmood Jasem Jawad, Mohammad Darvishi, Fahad Alsaikhan

{"title":"Bile's Hidden Weapon: Modulating the Microbiome and Tumor Microenvironment.","authors":"Mohamed J Saadh, Hanan Hassan Ahmed, Ali Fawzi Al-Hussainy, Irwanjot Kaur, Abhishek Kumar, Mamata Chahar, Suman Saini, Waam Mohammed Taher, Mariem Alwan, Mahmood Jasem Jawad, Mohammad Darvishi, Fahad Alsaikhan","doi":"10.1007/s00284-024-04004-0","DOIUrl":"10.1007/s00284-024-04004-0","url":null,"abstract":"The human gut microbiome is a dynamic and intricate ecosystem, composed of trillions of microorganisms that play a pivotal role in maintaining overall health and well-being. However, the gut microbiome is constantly exposed to various environmental factors, including the bile produced by the liver, which can significantly impact its composition and function. Bile acids, secreted by the liver and stored in the gallbladder, modulate the gut microbiome, influencing its composition and function. This altered microbiome profile can, in turn, impact the tumor microenvironment (TME), promoting an immunosuppressive environment that favors tumor growth and metastasis. Furthermore, changes in the gut microbiome can also influence the production of bile acids and other metabolites that directly affect cancer cells and their behavior. Moreover, bile acids have been shown to shape the microbiome and increase antibiotic resistance, underscoring the need for targeted interventions. This review provides a comprehensive overview of the intricate relationships between bile, the gut microbiome, and the TME, highlighting the mechanisms by which this interplay drives cancer progression and resistance to therapy. Understanding these complex interactions is crucial for developing novel therapeutic strategies that target the gut-bile-TME axis and improve patient outcomes.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 1","pages":"25"},"PeriodicalIF":2.3,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the Decreased Cholesterol Potential of Levilactobacillus brevis M-10 Isolated from Spontaneously Fermented Sour Porridge in Mice with High-Cholesterol Levels.

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-11-30 DOI: 10.1007/s00284-024-03974-5

Qi Wang, Jiawei Peng, Ye Tian, Jianhua Li, Jin Cai, Wenjun Qin

{"title":"Evaluation of the Decreased Cholesterol Potential of Levilactobacillus brevis M-10 Isolated from Spontaneously Fermented Sour Porridge in Mice with High-Cholesterol Levels.","authors":"Qi Wang, Jiawei Peng, Ye Tian, Jianhua Li, Jin Cai, Wenjun Qin","doi":"10.1007/s00284-024-03974-5","DOIUrl":"https://doi.org/10.1007/s00284-024-03974-5","url":null,"abstract":"Excessive cholesterol levels can lead to hypercholesterolemia, which is related to cardiovascular diseases (CVDs), and CVDs are a serious threat to human health. Therefore, lowering cholesterol levels is necessary, and diet intervention is safer than drugs are. The cholesterol-lowering effect of Levilactobacillus brevis M-10 isolated from spontaneously fermented millet sour porridge was investigated in fifty C57BL/6N male mice. After a 4-week intervention, the food intake, weight gains and organ indices were calculated; the lipid contents in the serum, liver, and feces were determined; the histopathology of the liver tissues was observed; the expression of metabolism-related genes was determined; and short-chain fatty acid (SCFA) levels in the droppings were monitored. The results showed that administration of a high dose of L. brevis M-10 (1 × 1010 CFU/mL) significantly reduced food intake, suppressed weight gain; prevented excessive liver growth; and reduced the total serum cholesterol, triglycerides, low-density lipoproteins; and total hepatic cholesterol and triglyceride contents (P < 0.05) in high-cholesterol mice. Moreover, a high dose of L. brevis M-10 significantly promoted the fecal excretion of cholesterol and triglycerides (P < 0.05) and alleviated liver damage induced by a high-cholesterol diet. Furthermore, a high dose of L. brevis M-10 significantly downregulated the cholesterol metabolism-related gene expression of NPC1L1, ACAT2, HMG-CoA, and SREBP2 but upregulated the gene expression of ABCG5, CYP7A1, and LXR-α (P < 0.05). Additionally, a high dose of L. brevis M-10 significantly increased SCFA contents, including those of acetic acid, propionic acid and n-butyric acid (P < 0.05). These findings could provide support for the use of L. brevis M-10 in the application of functional foods to alleviate hypercholesterolemia.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 1","pages":"24"},"PeriodicalIF":2.3,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pseudomonas chlororaphis ZH2: Evaluation of the Biocontrol Potential of Continuous Cropping Obstacles on the Basis of Genome Analysis, Autotoxic Substance Degradation and In Vitro Antifungal Activity.

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-11-29 DOI: 10.1007/s00284-024-03998-x

Rong Xiao, Chunfen Zhang, Shu Deng, Ciqiong Niu, Quan Li, Junjie Sun, Juantao Liu, Jianping Wang

{"title":"Pseudomonas chlororaphis ZH2: Evaluation of the Biocontrol Potential of Continuous Cropping Obstacles on the Basis of Genome Analysis, Autotoxic Substance Degradation and In Vitro Antifungal Activity.","authors":"Rong Xiao, Chunfen Zhang, Shu Deng, Ciqiong Niu, Quan Li, Junjie Sun, Juantao Liu, Jianping Wang","doi":"10.1007/s00284-024-03998-x","DOIUrl":"https://doi.org/10.1007/s00284-024-03998-x","url":null,"abstract":"The accumulation of autotoxic substances and fungal pathogens in soil are the two leading causes of continuous cropping obstacles. In this context, the use of beneficial strains for the biological control of continuous cropping obstacles is a promising research direction. In this work, the functions of Pseudomonas chlororaphis ZH2 in antagonizing pathogenic fungi and degrading autotoxic substances during continuous cropping were studied via genome-wide sequence analysis, antifungal activity in vitro, and autotoxic substances degrading tests. The results revealed that the genome of ZH2 contains 7,249,755 base pairs, has a GC content of 62.61%, and is predicted to contain 6551 genes. The phylogenetic results of 16S rRNA, atpD, recA, and carA gene analysis and the average nucleotide identity analysis revealed that ZH2 belongs to P. chlororaphis subsp. aurantiaca. KEGG analysis revealed that some genes of ZH2 were annotated to the biodegradation pathway of benzoate, p-hydroxybenzoic acid, and other autotoxic substances. Nineteen types of secondary metabolite biosynthesis-related gene clusters were predicted to exist in ZH2 via antiSMASH software, and it is predicted that ZH2 can produce a variety of secondary metabolites with antifungal and siderophore functions. The autotoxic substance degradation tests confirmed that ZH2 can degrade benzoate, p-hydroxybenzoic acid, vanillin, and vanillic acid, and the antagonism tests confirmed that ZH2 can inhibit the growth of pathogenic fungi such as Fusarium solani, Rhizopus oryzea, Pestalotiopsis mangiferae, Fusarium oxysporum f.sp. cucumerinum and Botryosphaeria dothidea. These results show that ZH2 degrades autotoxic substances and antagonizes pathogens. Therefore, it is a promising strain that can be used for the biological control of continuous cropping obstacles.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 1","pages":"21"},"PeriodicalIF":2.3,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142750272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whole-Genome Analysis of Multidrug-Resistant Klebsiella pneumoniae Kp04 Reveals Distinctive Antimicrobial and Arsenic-Resistance Genomic Features: A Case Study from Bangladesh.

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-11-29 DOI: 10.1007/s00284-024-03996-z

Md Javed Foysal, Farhana Momtaz, A M Masudul Azad Chowdhury, Afroza Akter Tanni, Asma Salauddin, Md Zahid Hasan, Sohana Akter Mina, Nahid Sultana, Sanjoy Kanti Biswas, Kamrul Islam, Alfred Tay, Adnan Mannan

{"title":"Whole-Genome Analysis of Multidrug-Resistant Klebsiella pneumoniae Kp04 Reveals Distinctive Antimicrobial and Arsenic-Resistance Genomic Features: A Case Study from Bangladesh.","authors":"Md Javed Foysal, Farhana Momtaz, A M Masudul Azad Chowdhury, Afroza Akter Tanni, Asma Salauddin, Md Zahid Hasan, Sohana Akter Mina, Nahid Sultana, Sanjoy Kanti Biswas, Kamrul Islam, Alfred Tay, Adnan Mannan","doi":"10.1007/s00284-024-03996-z","DOIUrl":"https://doi.org/10.1007/s00284-024-03996-z","url":null,"abstract":"Multidrug-resistant bacteria, particularly extended-spectrum-beta-lactamase-producing (ESBL) bacteria, pose a significant global public health challenge. Klebsiella pneumoniae (KPN) is frequently implicated in cases of this resistance. This study aimed to investigate the presence of drug and metal resistance genes in clinical K. pneumoniae isolate Kp04 and comparative genomics of clinical KPN isolates characterized from Bangladesh. A total of 12 isolates were collected. Disk-diffusion assay showed that all five isolates were resistant to 14 out of 21 tested antibiotics and sensitive to only three-tigecycline, imipenem, and meropenem. KPN Kp04 was positive for both blaSHV and blaCTX-M ESBL genes in PCR. All five isolates produced PCR amplicons of the correct size for ampicillin (ampC), tetracycline (tetC), fluoroquinolone (qnrS), and aminoglycoside (aadA) resistance genes. The whole genome of Kp04 was sequenced using the MiSeq Platform (V3 kit, 2 × 300 cycles). We utilized different databases to detect Antibiotic-Resistant Genes (ARGs), virulence factor genes (VFGs), and genomic functional features of the Kp04 strain. Whole-genome sequencing identified 75 ESBL, virulence, and multiple drug-resistant (MDR) genes including blaSHV, tetA, oqxA, oqxB, aadA, sul1-5, and mphA in KPN Kp04 isolate. Pan-genomic analysis of 43 Bangladeshi KPN isolates showed similarities between Dhaka and Chattogram isolates regarding virulence and antibiotic-resistant genes. Our results indicate the transmission of similar virulent KPN strains in Dhaka and Chattogram. This study would provide valuable information about drug sensitivity, antibiotic, and metal resistance features of K. pneumoniae circulated among hospitalized patients in Bangladeshi megacities.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 1","pages":"22"},"PeriodicalIF":2.3,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142754822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Closely Related Brucella Species Widely Differ in their Vegetative and Intracellular Growth.

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-11-29 DOI: 10.1007/s00284-024-03991-4

Nazarena Wade, Diego J Comerci, Alfonso Soler-Bistué, María Inés Marchesini

{"title":"Closely Related Brucella Species Widely Differ in their Vegetative and Intracellular Growth.","authors":"Nazarena Wade, Diego J Comerci, Alfonso Soler-Bistué, María Inés Marchesini","doi":"10.1007/s00284-024-03991-4","DOIUrl":"https://doi.org/10.1007/s00284-024-03991-4","url":null,"abstract":"Growth rate is a key prokaryotic trait that allows for estimating fitness and understanding cell metabolism. While it has been well studied in model organisms, there is limited data on slow-growing bacteria. In particular, there is a lack of quantitative studies on Brucella species. This genus includes important microorganisms that are causative agents of brucellosis, one of the most widespread bacterial zoonoses, affecting several species of animals, including humans. Brucella species exhibit approximately 97% genomic similarity. Despite this, Brucella species show different host preferences, zoonotic risks, and pathogenicity. After more than one hundred years of research, numerous aspects of Brucella biology, such as in vivo and in vitro growth, remain poorly characterized. In this work, we analyzed vegetative and intracellular growth of the classical Brucella species (B. abortus, B. melitensis, B. suis, B. ovis, and B. canis). Strikingly, each species displayed distinct growth parameters in culture. Doubling time (DT) ranged from 2.7 hs-1 in B. suis to 18 h-1 for B. ovis. In the context of intracellular infection of J774A.1 phagocytic cells, DT was longer, but it widely varied across species, closely correlating with the growth observed in vitro. Overall, and despite high genome similarity, we also found species-specific growth parameters in the intracellular cell cycle.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 1","pages":"20"},"PeriodicalIF":2.3,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142750353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0