Current Microbiology最新文献

{"title":"Hantaviruses as Emerging Zoonotic Pathogens: Molecular Biology, Epidemiology, Therapeutics, vaccine, and Pathogenesis.","authors":"Mohammad Mehdi Akbarin, Zahra Farjami, Hugo Ramírez Álvarez","doi":"10.1007/s00284-026-05194-5","DOIUrl":"10.1007/s00284-026-05194-5","url":null,"abstract":"<p><p>Hantaviruses are globally distributed zoonotic pathogens belonging to the family Hantaviridae and are responsible for two major human diseases, hemorrhagic fever with renal syndrome (HFRS) and hantavirus cardiopulmonary syndrome (HCPS). Despite substantial progress in hantavirus research, important challenges remain in understanding the interactions among viral evolution, reservoir ecology, environmental change, transmission dynamics, and host immunopathology. This review provides an integrated One Health perspective that connects molecular virology and host-virus interactions with transmission ecology, global epidemiology, and the mechanisms underlying vascular leakage and severe disease. In addition to summarizing established knowledge, we highlight recent advances in hantavirus taxonomy, genomic and molecular surveillance, ecological and transmission modeling, and the identification of hantavirus circulation in previously underrecognized regions. Particular emphasis is placed on the role of reservoir host dynamics, climate and environmental change, habitat disturbance, and human-wildlife interactions in shaping spillover risk, as well as on endothelial dysfunction, immune dysregulation, and coagulation abnormalities as interconnected mechanisms of disease severity. We also identify persistent knowledge gaps concerning host susceptibility, viral persistence, determinants of differential virulence and tissue tropism, interspecies transmission, and the development of effective antiviral therapies and vaccines. By integrating these complementary perspectives, this review provides a framework for understanding hantavirus emergence and pathogenesis while identifying priorities for future surveillance, prevention, and therapeutic research.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 12","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149227511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Detection Technologies for Helicobacter pylori Antibiotic Resistance: Current Status and Clinical Implications.","authors":"Zhenyu Wang, Jun Lin, Xuanni Zhang, Juan Yang, Yao Liu, Yuan Ji","doi":"10.1007/s00284-026-05136-1","DOIUrl":"10.1007/s00284-026-05136-1","url":null,"abstract":"<p><p>Helicobacter pylori (H. pylori) infects approximately half of the global population and is closely linked to chronic gastritis, peptic ulcers, and gastric cancer. Antibiotic resistance has become a major global health concern with marked regional variations, severely undermining empirical treatment efficacy. Resistance mechanisms primarily involve target gene mutations, efflux pump overexpression, and altered membrane permeability. This review summarizes resistance mechanisms of commonly used antibiotics against H. pylori, along with diagnostic approaches ranging from phenotypic to molecular methods, the latter offering faster and more precise mutation detection, yet limited by high costs and invasive sampling. Treatment has shifted from clarithromycin-based triple therapy to bismuth-containing quadruple and potassium-competitive acid blocker-based regimens. However, susceptibility-guided therapy faces implementation barriers, and empirical regimens remain prevalent. Future directions emphasize affordable multiplex molecular platforms, resistance surveillance networks, artificial intelligence-driven predictive models, and non-antibiotic adjunctive strategies. Integrating molecular diagnostics with antimicrobial stewardship to transition from empirical to precision-based management is critical for improving eradication rates and curbing further resistance spread.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 12","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13680304/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149223569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Large-Scale Comparative Genomics of 1,374 Lactiplantibacillus plantarum Genomes Reveals an Open Pan-genome and Strain-Level Variation in Probiotic-Associated and Safety-Related Genomic Features.","authors":"Wenhua Zhao, Hui Li, Maoling Hu, Tiansheng Bu","doi":"10.1007/s00284-026-05183-8","DOIUrl":"10.1007/s00284-026-05183-8","url":null,"abstract":"<p><p>Lactiplantibacillus plantarum is widely studied for food fermentation and probiotic applications, but its functional and safety-related characteristics are strongly strain dependent. Large-scale comparative genomics can characterize this diversity and support genome-informed strain prioritization, although genomic evidence alone does not establish probiotic efficacy or safety. A RefSeq genome collection of L. plantarum was subjected to accession-level auditing, exact-sequence dereplication, and predefined genome-quality filtering, yielding 1,374 unique genomes for final analysis. Genome characteristics, pan-genome structure, probiotic-associated annotation proxies, targeted sequence evidence, predefined safety-related genomic markers, isolation-source metadata, and COG functional annotations were evaluated. The final cohort had a mean genome size of 3.286 ± 0.126 Mb and a mean GC content of 44.419 ± 0.168%. Genome size and GC content showed a negative dataset-level association (Pearson's r = - 0.767; R² = 0.588). The observed pan-genome comprised 32,500 gene families, including 1,175 core and 31,325 accessory families, and remained non-saturating, with a Heaps' law exponent of γ = 0.289. Probiotic-associated annotation proxies showed heterogeneous prevalence, and targeted sequence analysis demonstrated that annotation nomenclature could substantially influence apparent marker detection. Evidence-supported antibiotic-resistance markers were detected in 46 genomes (3.35%) and mobile-genetic-element-associated markers in 93 (6.77%), whereas ldhD was detected in 1,372 genomes (99.85%). The predefined descriptive concern score assigned 1,236 genomes (90.0%) to the Lowest Genomic Concern category, 136 (9.9%) to the Low category, and two (0.1%) to the Moderate category. Only 1,327 of the 32,500 gene families received a recognized primary COG assignment. The quality-filtered L. plantarum RefSeq collection exhibits an open, accessory-rich pan-genome and substantial strain-level genomic diversity. Annotation-dependent marker detection, uneven database representation, and the limited discrimination of the descriptive concern score emphasize that genome-based screening should be used for candidate prioritization rather than as evidence of probiotic efficacy or definitive safety. Strain-specific phenotypic and application-relevant safety validation remains essential.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 12","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149223635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global Distribution and Co-occurrence of Antimicrobial, Biocide, and Metal Resistance Genes in 11,921 Acinetobacter baumannii Genomes.","authors":"Chenmiao Wu, Xiaomeng Yang, Wuzhen Yu","doi":"10.1007/s00284-026-05190-9","DOIUrl":"10.1007/s00284-026-05190-9","url":null,"abstract":"<p><p>Acinetobacter baumannii is a critical priority pathogen globally. While antimicrobial resistance genes (ARGs) are well characterized, the prevalence, distribution, and co-occurrence patterns of biocide and metal resistance genes (BMRGs) remain poorly defined at global scale. We performed genomic analysis of 11,921 non-redundant A. baumannii genomes retrieved from NCBI RefSeq, representing isolates from 93 countries collected between 1982 and 2025. ARGs and BMRGs were identified using AMRFinderPlus (v4.2.7; organism-specific mode). Multilocus sequence typing (Pasteur MLST) was performed, and BMRG-ARG co-occurrence was assessed using one-sided Fisher's exact tests for enrichment with Benjamini-Hochberg correction (q < 0.05). Every genome carried at least one AMR determinant, and 99.98% carried at least one biocide or metal resistance locus at initial annotation. AMRFinderPlus identified 687 resistance-associated loci (633 AMR determinants; 39 biocide and metal resistance loci, of which 38 BMRGs were retained for analysis after exclusion of the core-genome gene nreB; and 15 virulence-associated loci, which were excluded from resistance analyses). Sequence type 2 (ST2) accounted for 56.4% of genomes. The carbapenemase gene blaOXA-23 was detected in 62.6% of genomes, and fluoroquinolone resistance mutations were highly prevalent (gyrA S81L: 84.9%; parC S84L: 77.2%). Co-occurrence analysis identified 38 significant BMRG-ARG associations overall (q < 0.05, OR ≥ 10), with a prominent module linking the integron-associated marker qacEΔ1 to ARGs (e.g., sul1, aadA1); the population-level mercury resistance-tet(A) association was not supported in the phylogeny-based sensitivity analysis. This study provides quantitative evidence for structured BMRG-ARG co-occurrence patterns in A. baumannii, shaped by ST2 dominance. These patterns are consistent with possible linkage and co-selection, although the present data cannot establish physical linkage.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 12","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149220024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic Characterization of Multidrug-Resistant Enterobacter hormaechei from Bovine Raw Milk: Implications for Food Safety and Public Health.","authors":"Shruti J Joshi, Devangi B Mangroliya, Hetvi J Adhyaru, Jayesh H Kabariya, Vimal M Ramani","doi":"10.1007/s00284-026-05188-3","DOIUrl":"10.1007/s00284-026-05188-3","url":null,"abstract":"<p><p>Antimicrobial resistance in foodborne bacteria poses a growing threat to public health. In this study, multidrug-resistant Enterobacter hormaechei (E. hormaechei), a member of the Enterobacter cloacae complex (ECC), was isolated from bovine raw milk in Gujarat, India, and examined for its prevalence, antimicrobial resistance, and genomic characteristics. A total of 21 presumptive E. hormaechei isolates were recovered from 190 raw milk samples (11.05%) based on morphological and biochemical characteristics and further confirmed using a species-specific PCR assay. Based on their phenotypic antimicrobial resistance profiles, eight multidrug-resistant (MDR) isolates were selected for whole-genome sequencing and downstream genomic analyses. Whole-genome sequencing of the MDR isolates revealed distinct sequence types, with ST350 and ST68 clustered closely. The sequenced isolates harbored a diverse repertoire of antimicrobial resistance genes, including blaACT, qnrS1/qnrB7, fosA, oqxA/B, mph(A), and aminoglycoside-modifying enzymes, consistent with their multidrug-resistant phenotypes. Virulence profiling identified genes associated with iron acquisition, adhesion, stress response, and type VI secretion systems, suggesting the presence of virulence-associated genetic determinants. Plasmid replicons (IncFII and Col-type) and the insertion sequence IS26 were identified among the sequenced isolates. This study provides a genomic characterization of MDR E. hormaechei isolates from raw milk in Gujarat, India, and emphasizes the importance of continuous AMR surveillance to monitor the spread of Enterobacter lineages with relevance to food safety and public health.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 12","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149215062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbonic Anhydrases in Mycobacteria: Linking Metabolic Adaptation to Phenotypic Drug Tolerance.","authors":"Maneesh Kumar, Ashok Aspatwar","doi":"10.1007/s00284-026-05196-3","DOIUrl":"10.1007/s00284-026-05196-3","url":null,"abstract":"<p><p>Tuberculosis (TB) treatment is increasingly challenged by both antimicrobial resistance (AMR) and phenotypic drug tolerance, the latter representing a reversible physiological state that contributes to persistence and prolonged therapy. While AMR is driven by heritable genetic mutations, drug tolerance arises from reversible physiological adaptations that enable bacterial survival during prolonged antibiotic exposure. Increasing evidence suggests that metabolic remodeling plays a central role in establishing these drug-tolerant states. Carbonic anhydrases (CAs) are zinc-containing metalloenzymes that catalyze the reversible interconversion of carbon dioxide and bicarbonate, thereby maintaining intracellular pH homeostasis and supporting bicarbonate-dependent metabolism. Beyond their established metabolic functions, indirect evidence suggests that CAs may contribute to physiological adaptations associated with bacterial persistence under host-imposed stresses, including hypoxia, nutrient limitation, and acidic environments. Although they are not classical AMR determinants, CAs may influence the metabolic landscape associated with phenotypic drug tolerance. In this review, we examine current evidence supporting the role of CAs in mycobacterial metabolism and propose a conceptual framework linking bicarbonate homeostasis, intracellular pH regulation, and metabolic adaptation to bacterial persistence and drug tolerance. We further discuss the potential of carbonic anhydrase inhibitors (CAIs) as adjunctive agents whose ability to enhance the activity of conventional antitubercular drugs requires experimental validation, together with the value of experimental models, including the Mycobacterium marinum-zebrafish system, and computational approaches for validating CA-targeted therapeutic strategies. Overall, this review highlights CAs as candidate metabolic regulators that may influence the physiological state underlying phenotypic drug tolerance. Defining their mechanistic contribution to persistence could facilitate the development of metabolism-based therapeutic strategies that complement existing TB treatment.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 12","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13675255/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149206669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Broad-Spectrum Antagonistic Bacillus Velezensis Strain HY17 as a Potential Biocontrol Agent Aagainst Major Maize Fungal Diseases.","authors":"Yao Dong, Jiahe Liu, Xin Chen, Tianpeng Zhang, Xianming Zhou, Yi Han, Fuping Song, Jie Li, Xin Zhang","doi":"10.1007/s00284-026-05192-7","DOIUrl":"10.1007/s00284-026-05192-7","url":null,"abstract":"<p><p>Maize fungal diseases seriously threaten crop productivity and grain quality, highlighting the need for effective and sustainable biocontrol agents. In this study, an antagonistic bacterium, HY17, was isolated from maize rhizosphere soil and identified as Bacillus velezensis based on morphological characterization and phylogenomic analysis. HY17 exhibited broad-spectrum antifungal activity against several major maize fungal pathogens. Its cell-free fermentation supernatant showed clear concentration-dependent inhibition of fungal growth, indicating that extracellular metabolites play an important role in their antagonistic activity. HY17 also displayed broad growth adaptability under different culture media, temperature, and pH conditions. Whole-genome analysis revealed that HY17 contains a chromosome of 3,931,127 bp and multiple biosynthetic gene clusters associated with antimicrobial secondary metabolites. Crude lipopeptide extracts from HY17 further exhibited concentration-dependent antifungal activity. PCR assays detected representative antimicrobial biosynthetic genes, including bacA, bmyB, ituC, fenD, and srfAA, and LC-MS analysis revealed characteristic ion peaks putatively assigned to iturin-family lipopeptides and a fengycin-family component. Collectively, these results indicate that B. velezensis HY17 is a promising biocontrol candidate for maize fungal diseases, with lipopeptide-related metabolites likely contributing substantially to its broad-spectrum antifungal activity.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 12","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149180100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity and Functional Screening of Trichoderma Species from Soils in Nanchang, China.","authors":"Yanan Qiu, Lili Fan, Kehe Fu, Min Xiong, Xutang Tu, Zeyi Rao","doi":"10.1007/s00284-026-05180-x","DOIUrl":"10.1007/s00284-026-05180-x","url":null,"abstract":"<p><p>A total of 111 Trichoderma isolates were obtained from soil samples collected in Nanchang, China. Based on morphological characteristics and molecular phylogenetic analysis of the translation elongation factor 1-alpha (tef1) gene, these isolates were assigned to 16 distinct phylogenetic lineages closely related to known species, with T. semiorbis, T. asperellum, and T. koningiopsis being the most predominant, accounting for 29, 22, and 16 isolates, respectively. All isolates were functionally screened for the production of five industrially relevant enzymes (laccase, xylanase, cellulase, chitinase, and amylase) and for copper tolerance. Several strains exhibited remarkable enzymatic activities: T. asperellum TasJK96 demonstrated the highest phenol oxidase activity (1.95 ± 0.12 U·mL^- 1), T. virens TviJK51 excelled in xylanase production (41.49 ± 1.02 U·mL^- 1), T. afroharzianum Tafxt15 exhibited the highest cellulase activity (22.56 ± 0.70 U·mL^- 1), T. lentinulae TleJK44 was the top chitinase producer (22.21 ± 0.83 U·mL^- 1), and T. koningiopsis TkoJK88 demonstrated the highest amylase activity (91.26 ± 2.25 U·mL^- 1). Furthermore, 13 isolates displayed tolerance to copper, with T. virens TviJK10 exhibiting growth at concentrations up to 4.90 mmol·L^- 1. This study expands our understanding of Trichoderma diversity in the Nanchang region and provides a collection of promising candidate strains for future biotechnological and bioremediation applications.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 12","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149180108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic and Phenotypic Analysis of Antarctic Marine Cryobacterium sp. PAMC25264.","authors":"Anamika Khanal, So-Ra Han, Jun Hyuck Lee, Tae-Jin Oh","doi":"10.1007/s00284-026-05182-9","DOIUrl":"10.1007/s00284-026-05182-9","url":null,"abstract":"<p><p>Antarctic marine environments are extreme cold ecosystem and the microbes inhabiting them play a crucial role in ecosystem function. In this study, genomic and phenotypic analysis of Cryobacterium sp. PAMC25264, isolated from seawater of Barton, South Shetland Island, Antarctica Peninsula was performed. Comparative genomic analysis with related strains focused on nitrogen metabolic enzymes, carbohydrate-active enzymes (CAZymes) and stress-related proteins to elucidate the functional diversity and survival strategy of the strains in these harsh environments. Type Strain Genome Server (TYGS) analysis suggest that this strain may represent a distinct phylogenetic lineage within the genus Cryobacterium. Nitrogen metabolic enzymes including respiratory nitrate reductase (α, β, γ, and δ subunits), assimilatory nitrate reductase (NasA), nitrate/nitrite transporter (NarK/U), assimilatory nitrite reductase (NirB and NirD), and glutamate synthase (GltB and GltD) were identified from genomic analysis. dbCAN3 revealed the presence of a total of 125 CAZymes. Among them 66 glycoside hydrolases (GHs), 36 glycosyltransferases (GTs), 6 carbohydrate esterases (CEs), 15 carbohydrate-binding modules (CBMs), and 2 polysaccharide lyase (PL). In addition, proteins related to oxidative stress (superoxide dismutase, catalase, and thiol peroxidase), temperature stress (Csp and Hsp), and heavy metal(loid)s (HMMs) resistance were identified. Furthermore, the strain exhibited nitrate reduction in aerobic condition, resulting in nitrite accumulation, starch hydrolysis, and cellulose hydrolysis ability at different temperatures (4 °C, 10 °C, 15 °C, and 25 °C). Overall, these genomic and phenotypic traits indicate metabolic flexibility and functional diversity, supporting its survival and potential ecological role in extreme cold marine ecosystems.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 12","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149164431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bio-Transforming Sweet Potato into a Methionine-Rich Aquafeed Ingredient: A Statistical Screening of Saccharomyces cerevisiae Fermentation.","authors":"Yomna M Elshamy, Ahmed E Elshafey, Asmaa M El-Nokrashy, Radi A Mohamed, Bahaa Abdella","doi":"10.1007/s00284-026-05181-w","DOIUrl":"10.1007/s00284-026-05181-w","url":null,"abstract":"<p><p>For sustainable aquaculture production, a steady supply of high-quality protein for feed formulations is a challenge for countries with logistical or financial constraints on access to these resources. One of the most sustainable solutions to these challenges is to use microbial fermentation from readily available agricultural waste or crops. The aim of this study was to valorise sweet potatoes, a low-cost and abundant source of carbohydrates, by the baker's yeast Saccharomyces cerevisiae, to produce a protein enriched product suitable for aquaculture use. The Plackett-Burman design was used to effectively screen the key fermentation parameters that influencing the yield and results declared that ammonium sulphate concentration as the most crucial factor affecting the accumulation of protein. Carefully increasing its concentration resulted in a remarkable increase in the crude protein content of the fermented sweet potato (FSP), which increased to 46.24 ± 0.89%, compared with 3.88 ± 0.16% of raw sweet potato. Dietary analysis revealed significant improvements in the amino acid profile, notably a 70.08% increase in the essential amino acid methionine, as well as significant increases in threonine and alanine. This provides a solid basis for countries lacking high-quality protein for aquaculture to explore new local alternatives to achieve the required raw protein concentration from locally available sources..</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 12","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149147821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}