Current Microbiology最新文献

{"title":"Differential Microbial Composition and Fiber Degradation in Two Sloth Species (Bradypus variegatus and Choloepus hoffmanni).","authors":"Priscila Chaverri, Efraín Escudero-Leyva, Darling Mora-Rojas, Andrea Calvo-Obando, Mariana González, Esteban Escalante-Campos, Esteve Mesén-Porras, Daniela Wicki-Emmenegger, Diego Rojas-Gätjens, Judith Avey-Arroyo, Mariana Campos-Hernández, Erick Castellón, Andrés Moreira-Soto, Jan Felix Drexler, Max Chavarría","doi":"10.1007/s00284-025-04303-0","DOIUrl":"10.1007/s00284-025-04303-0","url":null,"abstract":"<p><p>Sloths have the slowest digestion among mammals, requiring 5-20 times longer to digest food than other herbivores, which suggests differences in their gut microbiota, particularly in plant-fiber-degrading microorganisms. Bradypus variegatus has a lower metabolic rate and moves less than Choloepus hoffmanni. However, no comprehensive studies have compared the microbiota (e.g., fungi) of these species. We hypothesized that differences in digestion and metabolism between the two species would be reflected in their microbiota composition and functionality, which we characterized using metagenomics, metabarcoding, and cellulose degradation. Results revealed significant differences in microbiota composition and functionality. Both species are dominated by bacteria; fungi comprised only 0.06-0.5% of metagenomic reads. Neocallimastigomycota, an anaerobic fungus involved in fiber breakdown in other herbivores, was found in low abundance, especially in B. variegatus. Bacterial communities showed subtle differences: C. hoffmanni was dominated by Bacillota and Bacteroidota, while B. variegatus showed higher Actinomycetota. Expected herbivore bacterial taxa (e.g., Fibrobacter and Prevotella) were scarce. Functional analysis showed a low abundance of carbohydrate-active enzymes essential for polysaccharide breakdown. Cellulose degradation assays confirmed that sloths digest only ~ 3-30% of ingested plant material. This research sheds light on the potential multidirectional links between the gut microbiota, metabolism, and digestion.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"327"},"PeriodicalIF":2.3,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting Metastasis: Exploring the Impact of Microbial Infections on Cancer Progression Through Innovative Biological Models.","authors":"Mohamed A El-Tayeb","doi":"10.1007/s00284-025-04306-x","DOIUrl":"10.1007/s00284-025-04306-x","url":null,"abstract":"<p><p>Infectious diseases, particularly viral infections, pose a significant threat to human health by contributing to cancer progression and metastasis. This review presents a novel perspective on how advanced biological models such as organoids, organ-on-a-chip systems, spheroids, and 3D bioprinting bridge critical translational gaps in research. These models replicate human physiological processes, allowing for the investigation of molecular mechanisms and the evaluation of novel therapeutics. This review will explore the current state of advanced biological models used to study viral infections associated with metastasis to better understand how viral-induced changes impact tumor behavior and the implications for future treatment strategies.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"328"},"PeriodicalIF":2.3,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical Analysis and Health Benefits of a Novel Polysaccharide Produced by Paenibacillus mucilaginosus.","authors":"Shimaa K Ali, Mohamed A Abdelgawad, Samy Selim, Doaa E Keshek, Hussein S Mohamed, Eman A Hamed","doi":"10.1007/s00284-025-04300-3","DOIUrl":"10.1007/s00284-025-04300-3","url":null,"abstract":"<p><p>Researchers have been fascinated by the remarkable properties of naturally occurring polysaccharides in recent years. We focused on extracting, refining, and analyzing an exopolysaccharide secreted for the first time by the bacterium Paenibacillus mucilaginosus, which was isolated from agricultural soil and identified by 16S rRNA sequence techniques. The polymer's elemental composition was analyzed, focusing on three primary constituents. Carbon emerged as the dominant element, comprising 29.76% of the polymer's total mass. Hydrogen was present at 4.98%, while nitrogen comprised 3.19% of the overall elemental makeup. Exopolysaccharide was characterized by FTIR, ¹HNMR, and 2D NOESY NMR spectroscopy. High-performance liquid chromatography analyses showed that the polysaccharide was a heteropolymer-containing rhamnose, fucose, galactose, and fructose as main sugars with 9.52, 8.03, 7.17, and 4.12 concentrations, respectively. The effects of various nitrogen and carbon sources on polymer production in batch culture medium were investigated. Fructose and yeast extract yielded the highest polymer dry weights at 9.4 and 7.3 g/L, respectively. Additionally, certain agricultural and industrial waste products were examined. The most significant polymer yield, nine g/L, was collected using a high-test molasses medium supplemented with yeast extract. This outcome was achieved after an incubation period of seventy-two hours at 30 °C in shaking flasks under batch culture conditions. The hepatoprotective efficacy of crude exopolysaccharide was assessed using the hepatoblastoma cell line. The exopolysaccharide demonstrated moderate hepatoprotective activity, with a protective effect of 34.61% observed at a concentration of 1000 µg/L.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"326"},"PeriodicalIF":2.3,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association with Elevated Somatic Cell Counts and Characterization of Aerococcus viridans Isolates from Bovine Mastitis Milk in South Korea.","authors":"Hye Jeong Kang, Ju-Yeon You, Seung Hoe Kim, Jin-San Moon, Ha-Young Kim, Jae-Myeong Kim, Young Ju Lee, Hyun-Mi Kang","doi":"10.1007/s00284-025-04291-1","DOIUrl":"10.1007/s00284-025-04291-1","url":null,"abstract":"<p><p>Aerococcus viridans, an emerging pathogen, is responsible for the recent increase in cases of bovine mastitis. However, its specific effects on mastitis remain largely unexplored. In this study, we examined the correlation between A. viridans-induced mastitis infections and somatic cell counts (SCCs), and characteristics of A. viridans isolates from bovine mastitis milk. Among 1774 mastitis milk samples collected between 2016 and 2021 in South Korea, 69 (3.9%) A. viridans isolates were obtained. Mastitis milk samples containing A. viridans exhibited significantly higher SCCs than did non-mastitis samples. Most isolates (80.5%) were associated with subclinical mastitis (200-1200 × 10³ cells/mL), whereas 19.5% were associated with clinical mastitis (> 1.2 × 10⁶ cells/mL). In pulsed-field gel electrophoresis analysis, A. viridans isolates displayed substantial genetic diversity, with no dominant clones identified. Antimicrobial susceptibility testing revealed high resistance rates to ceftiofur (46.4%) and oxacillin + 2% NaCl (44.9%) among β-lactams, followed by tetracycline (36.2%) and erythromycin (10.1%), with 21.7% isolates being multidrug-resistant. Fifty-four isolates (78.3%) were able to form biofilms, with all recent isolates being biofilm-positive, in contrast to several earlier non-producers. Our findings suggest the necessity for targeted management strategies and continuous monitoring for mitigating A. viridans-induced mastitis in dairy cows.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"325"},"PeriodicalIF":2.3,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12143986/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144246957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic Effects of 5-Ureidohydantoin, Rhizobacteria and Non-wood-Based Biochar on Barley Productivity Under Drought and Multiple Toxic Metals Stress.","authors":"Ghulam Murtaza, Muhammad Usman, Muhammad Azam, Abdul Razzaq, Iram Saba, Gang Deng, Zeeshan Ahmed, Abdel-Rhman Z Gaafar, Trobjon Makhkamov, Lala Gurbanova, Rashid Iqbal","doi":"10.1007/s00284-025-04305-y","DOIUrl":"10.1007/s00284-025-04305-y","url":null,"abstract":"<p><p>Barely (Hordeum vulgare L.), an essential crop accounting for roughly 15% of global caloric consumption, confronts substantial risks from toxic metal pollution, especially drought stress, (Pb) and mercury (Hg) stress, endangering global food safety. The current research sought to examine synergistic impacts of abovementioned stressors and efficacy of 5-ureidohydantoin, rhizobacteria and non-wood-based biochar in promoting barley growth. Experimental design comprised 24 treatment combinations featuring drought stress, Pb stress (5 mg/kg) and Hg stress (250 and 500 mg/kg) with diverse administrations of biochar and 5-ureidohydantoin. Rhizobacteria and 5-ureidohydantoin combination increase barley growth by promoting root development, enhancing nutrient absorption and improving overall vitality, with 5-ureidohydantoin facilitating cell division and elongation, while rhizobacteria enhance nutrient accessibility and root integrity, resulting in an expedited germination process, flowering and yields. Barley seeds cv. TJ-70, subjected to Agrobacterium rhizogenes treatment, exhibited notable growth parameters, with root lengths measuring 10.01 cm under 5 mg/kg Pb stress than 6.31 cm in control group. The treatment enhanced root and shoot FW by 23.7% 25.71%, respectively, whereas chlorophyll content reached a maximum of 2.69 mg/g at 5 mg/kg Pb. Research revealed that plants exposed to rhizobacteria and 5-ureidohydantoin had substantial enhancements in germination rate (over 85%), root length (averaging 27 cm), shoot length (averaging 29 cm) and total biomass in comparison to control treatments. The simultaneous application of 5-ureidohydantoin and jute-biochar exhibited significant promise for enhancing barley's resistance to metals and drought stresses by increased chlorophyll levels and decreased EL in amended plants. Furthermore, electrolyte leakage diminished to 11.4%, and the vigor index enhanced to 1601.09 under Pb stress. These data suggest that biochar and 5-ureidohydantoin use can alleviate detrimental impacts of environmental stresses on barley. Extending this study to encompass various crops will facilitate evaluation of wider relevance of these results; thereby fostering integrated crops management tactics utilize stimulants such as rhizobacteria and 5-ureidohydantoin in conjunction with organic and inorganic amendments, ultimately enhancing agricultural resilience and global food security.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"324"},"PeriodicalIF":2.3,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144233512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbapenem Resistant Serratia marcescens Harboring bla_OXA-48, armA and aac(6')-Ib-cr Genes: The First Baseline Study from Pakistan.","authors":"Amna Kausar, Muhammad Sohail, Mohsin Khurshid, Hafiz Ghulam Murtaza Saleem","doi":"10.1007/s00284-025-04302-1","DOIUrl":"10.1007/s00284-025-04302-1","url":null,"abstract":"<p><p>The emergence of Serratia marcescens as a significant healthcare-associated pathogen is compounded by its rising carbapenem resistance resulting in a critical global health challenge. This study is the first in Pakistan to comprehensively characterize the antimicrobial resistance patterns and molecular mechanisms of S. marcescens from clinical specimens. The S. marcescens isolates were obtained from clinical specimens collected at tertiary care hospitals in Lahore and Faisalabad, Pakistan, between October 2023 and September 2024. Isolates were identified using the Vitek-2 system and MALDI-TOF mass spectrometry. The disk diffusion assays, and broth microdilution method were used for antimicrobial susceptibility testing. The antimicrobial resistance determinants were amplified by PCR followed by sanger sequencing. The study revealed concerning resistance rates among S. marcescens isolates: 78% were resistant to third-generation cephalosporins, 52% to carbapenems, and 71.6% to fluoroquinolones. However, no isolates were found to be resistant to the tigecycline. The carbapenem resistance genes were distributed among carbapenem-resistant S. marcescens (CRSM) strains as follows: bla_OXA-48 was most prevalent at 69.2%, followed by bla_KPC at 20.8%, and bla_IMP at 10% of isolates. The 16S methylase gene armA was found exclusively in CRSM strains, present in 73.1% (95/130) of isolates. The aac(6')-Ib-cr gene variant, which confers resistance to aminoglycosides and fluoroquinolones, was found in 37.2% of CRSM isolates. The CRSM isolates co-harboring bla_OXA-48, armA, and aac(6')-Ib-cr genes demonstrated a convergence of carbapenem, aminoglycoside, and fluoroquinolone resistance mechanisms which confer a multi-drug resistance phenotype in these isolates, with tigecycline remaining the sole susceptible agent. These findings expose an escalating AMR crisis in Pakistan driven by high-risk resistance gene combinations among S. marcescens isolates. This underscores the dire necessity for urgent nationwide interventions including rapid diagnostics, genomic surveillance of resistance mechanisms, and strict antibiotic stewardship to curb the increasing antimicrobial resistance threats and nosocomial transmission of such pathogens.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"321"},"PeriodicalIF":2.3,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prospects of Synthetic Biology-Based Approaches in the Enhanced Production of Pyocyanin in Pseudomonas aeruginosa MCCB 117 as the Drug of Choice in Aquaculture.","authors":"Jini Jimmy, Jayesh Puthumana, Valsamma Joseph, I S Bright Singh","doi":"10.1007/s00284-025-04279-x","DOIUrl":"10.1007/s00284-025-04279-x","url":null,"abstract":"<p><p>Pyocyanin, the nitrogen-containing heterocyclic phenazine compound produced by Pseudomonas aeruginosa, has been proven to be a potential drug of choice against vibriosis in sustainable aquaculture production systems. Considering the cost of production and the fact that pyocyanin is produced in very small quantities in nature genetic improvement of bacterial strains for enhanced production of pyocyanin is a requirement. In-depth studies on the key biomolecules behind the biosynthesis of pyocyanin in P.aeruginosa has been conducted previously, however, gene level manipulations of the pyocyanin biosynthetic pathway has to be experimented enhancing the yield in P.aeruginosa. In this review, we look in to the molecular mechanism behind pyocyanin production in P. aeruginosa and an attempt has been made to investigate the growing scope of microbial synthetic biology in pyocyanin biosynthesis. In the present scenario where, synthetic biology-based tools are gaining much importance, this review throws light to building up a platform for synthetic biology-based production of pyocyanin.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"322"},"PeriodicalIF":2.3,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineered Bacteria-Mediated Delivery of Scorpion Venom Peptide AGAP for Targeted Breast Cancer Therapy.","authors":"Yang Zhang, Xianglong Li, Chuanjun Tian, Chunyan Zhong, Wenwen Li, Xiaobing Shang, Minghui Zhao, Yongshan Zhao","doi":"10.1007/s00284-025-04289-9","DOIUrl":"10.1007/s00284-025-04289-9","url":null,"abstract":"<p><p>The rising incidence and mortality rates of malignant tumors highlight their profound impact on human health. Bacterial therapy has emerged as a promising avenue in oncological research. Our lab has isolated an analgesic-anti-tumor peptide from the venom of Buthus martensii Karsch (BmK AGAP), a long-chain scorpion venom peptide, which exhibits remarkable anti-tumor activity. However, the limited bioavailability of peptides poses a challenge for their therapeutic efficacy. To address this challenge, we focused on enhancing the delivery of BmK AGAP to improve its anti-tumor effectiveness. We engineered E. coli K12 to create the TSYPU strain, which not only expresses BmK AGAP, but also possesses lytic capabilities. Co-culturing of TSYPU with murine breast cancer 4T1 cells in vitro demonstrated its potential as a drug delivery platform. Further advancements included the encapsulation of TSYPU with nanogold particles, resulting in TSYPU@Au strain. In vivo experiments revealed that TSYPU@Au exhibited a significant anti-tumor effect, crucially overcoming degradation in the acidic gastrointestinal environment. In summary, our study highlights the viability of engineered TSYPU bacteria as carriers for BmK AGAP delivery, offering a promising approach for the rational design of bacterial-based peptide drug delivery systems in oncology. This strategy has considerable potential for advancing the field and warrants further investigation in future studies.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"323"},"PeriodicalIF":2.3,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metagenomic Analysis of Pulp and Paper Wastes and Prospects for Their Self-purification.","authors":"Yulia Kocharovskaya, Yanina Delegan, Sergei Sevostianov, Alexander Bogun, Dmitry V Demin","doi":"10.1007/s00284-025-04308-9","DOIUrl":"10.1007/s00284-025-04308-9","url":null,"abstract":"<p><p>Thousands of tons of waste accumulate, as a result of the activities of the pulp and paper industry, which is often stored in the form of dumps. However, intensifying the use of lignocellulose for more efficient bioremediation remains highly challenging. Therefore, the study of microbiomes with potentially desirable characteristics for the decomposition of pulp and paper wastes is currently an important task. In this study, a comprehensive assessment of the microbiota biodiversity of these dumps was carried out using high-throughput, high-resolution sequencing. In study 472 million high-quality clean reads assembled into 6,413,337 contigs with a total length of 4306 Mb, of which 3,633,174 open reading frames (ORFs) were identified. The core microbiome was composed of four phyla from Proteobacteria, Actinobacteria, Bacteroidetes, and Verrucomicrobia. Representatives of phylum Proteobacteria prevailed in samples. Annotation using the KEGG database in the Metabolism category resulted in 654,234 ORFs and 5138 ORFs encoding enzymes/proteins involved in degradation of lignocellulose which formed main pool of the wastes. By use of the created database, the search for lignocellulose degradation genes showed that genera Shewanella, Achromobacter, and Delftia covered significant part of the reads. The results indicate that the established microbiome of local landfills can be considered as an important source for improving lignocellulose bioremediation, provided that lignocellulosic fungi are sufficiently active. In whole, these new data can be used as a scientific basis to form an efficient eco-biotechnology for auto-remediation of pulp and paper industry waste.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"320"},"PeriodicalIF":2.3,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating c-di-GMP Signaling in Vibrio parahaemolyticus: Biological Effects and Mechanisms of Regulation.","authors":"Pu Yao, Yiquan Zhang, Jun Ding","doi":"10.1007/s00284-025-04260-8","DOIUrl":"10.1007/s00284-025-04260-8","url":null,"abstract":"<p><p>Vibrio parahaemolyticus is a halophilic, Gram-negative bacterium found in marine and estuarine ecosystems and is a significant contributor to seafood-related bacterial gastroenteritis. Cyclic di-GMP (c-di-GMP) is a widespread secondary messenger that modulates various cellular pathways, including virulence factor production, motility, and biofilm formation. c-di-GMP is made by diguanylate cyclases (DGCs), while its breakdown is mediated by phosphodiesterases (PDEs). Although many putative DGCs and PDEs involved in c-di-GMP metabolism have been identified in V. parahaemolyticus, only a few have been thoroughly studied. Furthermore, the metabolism of c-di-GMP is influenced by various environmental factors and transcriptional regulators. This review provides an overview of the c-di-GMP signaling network in V. parahaemolyticus, focusing on recent advancements related to c-di-GMP metabolic enzyme genes and their regulatory mechanisms. Understanding the intricate mechanisms of c-di-GMP metabolism and regulation is crucial for revealing the environmental adaptation and pathogenic strategies of V. parahaemolyticus.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"319"},"PeriodicalIF":2.3,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144198534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}