Current Microbiology最新文献_第4页

Adaptive Genomic Features of Raoultella ornithinolytica LAM1 from the Geothermal Site of Los Azufres Reveal Potential for Heavy-Metal Bioremediation. 来自Los azures地热遗址的鸟类溶解Raoultella ornithinolytica LAM1的适应性基因组特征揭示了重金属生物修复的潜力

IF 2.6 3区生物学

Current Microbiology Pub Date : 2025-09-28 DOI: 10.1007/s00284-025-04507-4

Grisel Fierros-Romero, Mauricio Nahuam Chávez-Avilés, Mariana Hoflack-Culebro, Cassandra Orozco-Neri, Darlene A Juárez-Beltrán, Mónica M Luviano-Reyes, Ilse Macedo-Ramírez, Marcos De Donato-Capote, Hectorina Rodulfo, David Enrique Zazueta-Alvarez, Ernesto Rodríguez-Andrade, Dianella Iglesias, Ashutosh Sharma

{"title":"Adaptive Genomic Features of Raoultella ornithinolytica LAM1 from the Geothermal Site of Los Azufres Reveal Potential for Heavy-Metal Bioremediation.","authors":"Grisel Fierros-Romero, Mauricio Nahuam Chávez-Avilés, Mariana Hoflack-Culebro, Cassandra Orozco-Neri, Darlene A Juárez-Beltrán, Mónica M Luviano-Reyes, Ilse Macedo-Ramírez, Marcos De Donato-Capote, Hectorina Rodulfo, David Enrique Zazueta-Alvarez, Ernesto Rodríguez-Andrade, Dianella Iglesias, Ashutosh Sharma","doi":"10.1007/s00284-025-04507-4","DOIUrl":"https://doi.org/10.1007/s00284-025-04507-4","url":null,"abstract":"Raoultella ornithinolytica strain LAM1, a facultative anaerobe isolated from a metal-rich geothermal pond in Los Azufres, Mexico, grew in sodium arsenate concentrations up to 1500 ppm (7.215 mM/L). Whole-genome sequencing yielded a 6.01-Mbp draft genome across 104 contigs, encoding 5744 predicted genes annotated using Prokka and NCBI PGAP. Among these, 99 genes were associated with resistance to arsenic, mercury, copper, zinc, cobalt, cadmium, nickel, and lead. We identified a complete ars operon (arsR-arsB-arsC-arsA-arsD) with three arsC paralogs; mer operon genes (four merA copies, merR); zntA-zntR; copA-cueO-cusA; and the metal homeostasis system nikABCDE-nikR. Functional classification assigned 27.9% of resistance genes to zinc, 20.9% to nickel, 18.6% to copper, and 16.3% to arsenic. Cluster of orthologous genes (COG) annotation revealed enrichment in ABC-type permeases (COG0601/1173/0444), metal ion efflux systems (COG1566), and redox enzymes. Growth on CHROMagar™ ESBL medium indicated β-lactamase activity. Comparative analysis with 508 publicly available R. ornithinolytica genomes confirmed conservation of core resistance operons and identified adaptations in sulfur metabolism (dsrB). These results support R. ornithinolytica strain LAM1's survival in metal-contaminated geothermal environments and indicate potential for bioremediation applications.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 11","pages":"532"},"PeriodicalIF":2.6,"publicationDate":"2025-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145184854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial Pneumonia Alters the Respiratory Microbiota and Inflammatory Profile Via the Lung Microbiome-Immune Axis. 细菌性肺炎通过肺微生物-免疫轴改变呼吸道微生物群和炎症谱。

IF 2.6 3区生物学

Current Microbiology Pub Date : 2025-09-27 DOI: 10.1007/s00284-025-04482-w

Xiaoyi Ye, Binghan Shen, Zhiming Cai, Dongdong Chen, Yanling Wu

{"title":"Bacterial Pneumonia Alters the Respiratory Microbiota and Inflammatory Profile Via the Lung Microbiome-Immune Axis.","authors":"Xiaoyi Ye, Binghan Shen, Zhiming Cai, Dongdong Chen, Yanling Wu","doi":"10.1007/s00284-025-04482-w","DOIUrl":"10.1007/s00284-025-04482-w","url":null,"abstract":"Bacterial invasion of the lungs causes pneumonia, which poses a serious threat to public health. In the absence of pathogen identification, the misuse of antibacterial agents contributes to bacterial resistance. This study investigated changes in the respiratory microbiome and proinflammatory profiles in bronchoalveolar lavage fluid (BALF) from patients with bacterial pneumonia before and after treatment, compared with healthy controls. Leukocyte count, granulocyte count, and neutrophil percentage were significantly elevated in patients with bacterial pneumonia. Microbial species richness in the untreated pneumonia group (group A) was significantly lower than in the treated group (group B) and healthy controls (group C). Significant differences in α-diversity and β-diversity were observed across all three groups. Linear discriminant analysis effect size (LEfSe) identified 19 microbial biomarkers at the genus level. Levels of TNF-α and IL-6 in BALF and serum were significantly reduced in group A following treatment, as measured by ELISA. Correlation analysis revealed associations between proinflammatory cytokines and several genera, including P5D1_392, Chloroplast, Blautia, Flavonifractor, Terrisporobacter, [Ruminococcus]_gauvreauii_group, Anaerostipes, Ruminococcus, Parabacteroides, Ellin6055, and Clostridia_UCG-014. These findings suggest that bacterial pneumonia disrupts the respiratory microbiota and alters the inflammatory profile through the lung microbiome-immune axis, leading to immune dysregulation. This work provides a theoretical basis for targeted treatment strategies in bacterial pneumonia.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 11","pages":"528"},"PeriodicalIF":2.6,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12476438/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145174215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endophytes in Medicinal Plants: A Sustainable Solution for Coping with Environmental Stresses. 药用植物内生菌：应对环境胁迫的可持续解决方案。

IF 2.6 3区生物学

Current Microbiology Pub Date : 2025-09-27 DOI: 10.1007/s00284-025-04508-3

Anshu Upadhyay, Vishal Khandelwal

{"title":"Endophytes in Medicinal Plants: A Sustainable Solution for Coping with Environmental Stresses.","authors":"Anshu Upadhyay, Vishal Khandelwal","doi":"10.1007/s00284-025-04508-3","DOIUrl":"https://doi.org/10.1007/s00284-025-04508-3","url":null,"abstract":"The increasing need for integrative and alternative medical therapies, especially in the aftermath of the COVID-19 epidemic, has emphasized the importance of medicinal plants in worldwide healthcare. These plants, which contain abundant bioactive secondary metabolites, provide a sustainable and cost-effective option for medicinal, adaptogenic, and immune-boosting purposes. Blooming medicinal plants that exist are at risk of becoming extinct because of excessive harvesting, deforestation, and wildfires. Medicinal plants have complex physiological defenses against stress, which are strengthened by their symbiotic relationship with endophytes. Endophytes are microbial colonies that live within plant tissues without causing harm and play a vital role in maintaining the health of plants by helping them to tolerate stress, promoting development, acquiring nutrients, synthesizing phytohormones, breaking down toxic substances, and improving plant resistance to environmental pressures such as high salt levels, lack of water, and exposure to heavy metals. In addition, endophytes have a role in managing biotic stress by engaging in antibiosis, synthesizing lytic enzymes, producing secondary metabolites, and regulating hormones. Their function in preserving the health and well-being of the host, ensuring proper nutrition intake, and enhancing resistance against pathogens highlights their potential as agents for biological control and biofertilization, providing a safer option compared to chemical pesticides. Endophytic inoculants have the potential to significantly transform crop yield in agriculture by reducing the impact of abiotic problems and improving soil health. This review critically evaluates causal studies and recent omics-based advances, highlighting their crucial significance for sustainable bioinoculant development and practical applications in climate-resilient agriculture.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 11","pages":"529"},"PeriodicalIF":2.6,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145174210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maple Syrup and Bacillus velezensis based Supplement: in Vivo Study of the Impact on Growth Performance, Microbiota Composition, and Metabolic Activity in Weaned Piglets. 添加枫糖浆和芽孢杆菌对断奶仔猪生长性能、微生物群组成和代谢活性影响的体内研究

IF 2.6 3区生物学

Current Microbiology Pub Date : 2025-09-27 DOI: 10.1007/s00284-025-04514-5

Gautier Decabooter, Luca Lo Verso, Frederic Guay, Marie Filteau, Ismail Fliss

{"title":"Maple Syrup and Bacillus velezensis based Supplement: in Vivo Study of the Impact on Growth Performance, Microbiota Composition, and Metabolic Activity in Weaned Piglets.","authors":"Gautier Decabooter, Luca Lo Verso, Frederic Guay, Marie Filteau, Ismail Fliss","doi":"10.1007/s00284-025-04514-5","DOIUrl":"10.1007/s00284-025-04514-5","url":null,"abstract":"Post-weaning piglets often face digestive challenges and growth setbacks due to gut microbiota imbalances and increased susceptibility to infections and diarrhea. Various alternatives to antibiotic growth promoters have been proposed, including synbiotic supplements. Maple syrup is a source of prebiotic compounds, while Bacillus velezensis FZB42 is a nonpathogenic microorganism with probiotic potential. Therefore, this study investigated the effects of a fermented ingredient combining maple syrup and B. velezensis as a potential synbiotic. The focus was on growth performance, occurrence of diarrhea, short-chain fatty acid production, and intestinal microbiota composition in weaned piglets. A two-week experiment in a randomized complete block design was conducted with the following treatments: a negative control diet (NC); a positive control diet containing chlortetracycline hydrochloride (PC); a synbiotic supplement containing maple syrup and B. velezensis (SYN); and a freeze-dried maple syrup supplement (FMS). Compared to the NC group, SYN supplementation increased final body weight, average daily gain, and decreased the feed conversion ratio (p < 0.05). The results showed that dietary supplementation with SYN supplement increased butyric acid concentrations in the ileum compared to NC (p < 0.014) and increased acetic (p < 0.001) and butyric acids (p < 0.044) in the colon compared to PC treatment. Additionally, both maple syrup-based supplements favorably modulated the relative abundance of microbial taxa, increasing Oscillisibacter and reducing Campylobacter, among others. Our findings indicate that dietary supplementation with a synbiotic composed of B. velezensis FZB42 and maple syrup improved the growth performance of weanling piglets, increased acetic and butyric acid content in the colon and butyric acid in the ileum, and favorably modulated the intestinal microbiota.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 11","pages":"530"},"PeriodicalIF":2.6,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12476450/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145174253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Host-Driven Functional Divergence in Gut Microbiota of Honeybees Apis cerana and Apis mellifera: Implications for Pollination, Nutrition, and Sustainable Apiculture. 宿主驱动的蜜蜂肠道微生物群功能分化：对授粉、营养和可持续养蜂的影响。

IF 2.6 3区生物学

Current Microbiology Pub Date : 2025-09-27 DOI: 10.1007/s00284-025-04525-2

M R Raiza Nazrin, J S Pavan, M N Rudra Gouda, K M Kumaranag, Sachin S Suroshe, Deeba Kamil, Sabtharishi Subramanian

{"title":"Host-Driven Functional Divergence in Gut Microbiota of Honeybees Apis cerana and Apis mellifera: Implications for Pollination, Nutrition, and Sustainable Apiculture.","authors":"M R Raiza Nazrin, J S Pavan, M N Rudra Gouda, K M Kumaranag, Sachin S Suroshe, Deeba Kamil, Sabtharishi Subramanian","doi":"10.1007/s00284-025-04525-2","DOIUrl":"https://doi.org/10.1007/s00284-025-04525-2","url":null,"abstract":"Honeybees are indispensable in sustaining natural ecosystems and global crop production by pollinating key food crops. Understanding their gut microbiota is crucial for insights into their health, nutrition, and behavior, with broader ecological and agricultural relevance. This study compares the gut microbiota of Apis cerana and Apis mellifera, focusing on bacterial composition, diversity, and functional roles. Using culture-dependent and metagenomic methods, core bacteria such as Lactobacillus, Fructobacillus, Gilliamella apicola, Bartonella apis, and Snodgrassella alvi were identified, linked to carbohydrate and nitrogen metabolism, immune modulation, and polysaccharide degradation. A. mellifera exhibited greater microbial and functional diversity, reflected in higher Shannon (1.22 vs. 1.08) and Simpson (0.675 vs. 0.655) indices. Strong intraspecies correlations and weaker interspecies correlations (Pearson's r = 0.6486) indicated distinct microbial profiles, supported by PCA (75.3% variation) and Adonis test (P = 0.04, R2 = 0.723). Functional analysis via MG-RAST and UniFrac-based PCoA showed species-specific differences in key metabolic pathways. Enzymatic profiling revealed Fructobacillus fructosus with high invertase activity (7.31 ± 0.30) and Apilactobacillus apinorum with strong pectinolytic activity (4.707 ± 0.36), enhancing honeybee nutrition. These findings have significant implications for pollination efficiency, probiotic development, and sustainable apiculture, ultimately supporting conservation strategies and the resilience of honeybee populations.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 11","pages":"531"},"PeriodicalIF":2.6,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145181850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Commercial and Paenibacillus tarimensis Exopolysaccharides on Soil Aggregation and Hydraulic Properties of a Saline Clay Loam Soil. 商品和塔里芽孢杆菌胞外多糖对盐渍粘壤土土壤团聚性和水力特性的影响。

IF 2.6 3区生物学

Current Microbiology Pub Date : 2025-09-26 DOI: 10.1007/s00284-025-04509-2

Lynda Smail, Nadjette Djemouai, Nadia Boukhelata, Nassima Baha, Yahia Kaci

{"title":"Effects of Commercial and Paenibacillus tarimensis Exopolysaccharides on Soil Aggregation and Hydraulic Properties of a Saline Clay Loam Soil.","authors":"Lynda Smail, Nadjette Djemouai, Nadia Boukhelata, Nassima Baha, Yahia Kaci","doi":"10.1007/s00284-025-04509-2","DOIUrl":"https://doi.org/10.1007/s00284-025-04509-2","url":null,"abstract":"Soil structure and its stability are crucial for water flux and retention, as well as for plant growth and the control of soil erosion, especially in arid regions. Microbial exopolysaccharides (EPS) play a key role in soil aggregation due to their rheological properties, particularly their viscosity, elasticity, and polyelectrolyte nature. EPS-R1 was obtained from Paenibacillus tarimensis REG 0201M, which was isolated from Reggane (Adrar Province, Algeria). The EPS was evaluated for its effects on the physical properties of a calcareous, silty-clay, saline soil, including soil aggregate formation, its stability, and the soil permeability. The bacterial EPS-R1 was produced on Yeast Extract Sucrose Agar, and then both bacterial cells and EPS were recovered and mixed with the soil at three concentrations: 0.2, 0.4, and 0.6% (w/w). Commercial plant and microbial biopolymers viz., gum Arabic and agar-agar, and xanthan gum, respectively, were used to compare their effects. EPS-R1 enhanced macroaggregation markedly at concentrations as low as 0.2%, achieving up to 86.89% of macroaggregates greater than 2 mm at 0.6%. Aggregate stability also improved, with only 12.8% disintegration at 0.6% EPS-R1, when compared with 29.8% with XG. The mean weight diameter showed that treatment with EPS-R1 transformed the soil from a very unstable to a highly stable structure. Furthermore, soil permeability increased by 251% at 0.6%. The water loss kinetics indicated that soil water retention forces decreased with the addition of 0.2% EPS-R1, thereby improving soil aggregate stability. The findings highlighted the potential of EPS-R1 as an effective bio-structuring soil amendment for arid and degraded salt-affected soils.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 11","pages":"527"},"PeriodicalIF":2.6,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145148329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitigating Virulence of Environmental Enterococcus faecalis Via Fermented Food-Derived Lactic Acid Bacteria. 通过发酵食物来源的乳酸菌减轻环境粪肠球菌的毒力。

IF 2.6 3区生物学

Current Microbiology Pub Date : 2025-09-26 DOI: 10.1007/s00284-025-04501-w

Mohammed Aman, Sushma S Murthy, Arpitha Somayaji

{"title":"Mitigating Virulence of Environmental Enterococcus faecalis Via Fermented Food-Derived Lactic Acid Bacteria.","authors":"Mohammed Aman, Sushma S Murthy, Arpitha Somayaji","doi":"10.1007/s00284-025-04501-w","DOIUrl":"https://doi.org/10.1007/s00284-025-04501-w","url":null,"abstract":"Enterococcus faecalis, a Gram-positive bacterium commonly found in environmental reservoirs, poses growing concerns due to its adaptability, virulence traits, and emerging resistance to critical antibiotics. In this study, eight Enterococcus strains (JUSS 01-03, 05-07, 09, and 10) were isolated from irrigation water contaminated with sewage around Bengaluru, India. All isolates were subjected for antibiotic susceptibility testing, where, JUSS 05 (E. faecalis) and JUSS 06 (E. faecalis) exhibited ampicillin resistance (1.5 and 2.5 mm zones), suggesting the isolates to be antibiotic-resistant with high virulence. This virulent activity is further supported by cell surface hydrophobicity and auto-aggregation showing the highest hydrophobicity and aggregation, respectively. JUSS 06 and JUSS 05 exhibited the strongest biofilm formation (~ 0.65 OD), linking hydrophobicity to biofilm biomass. Probiotic interventions isolated from fermented food, using Pediococcus acidilactici (JUFF, JUFB) and Streptococcus thermophilus (JUBM) significantly reduced virulence traits across isolates. JUBM had the greatest impact on reducing hydrophobicity, aggregation, and biofilm, likely via exopolysaccharide production and surface interference. JUFF demonstrated consistent and broad-spectrum anti-biofilm activity against JUSS 05, JUSS 06, and the clinical strain CV2656 (P < 0.0001). The reduction of hydrophobicity and aggregation by lactic acid bacteria (LAB) indicates disruption of early biofilm development stages and interference with adhesion mechanisms. These findings emphasize the pathogenic potential of environmental E. faecalis and highlight the effectiveness of food-derived LAB in reducing virulence factors. Probiotic applications could serve as sustainable bio-interventions against environmental isolates of enterococcal species, further investigation is needed to evaluate their application potential.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 11","pages":"523"},"PeriodicalIF":2.6,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145148249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogenetic Studies on Taurine Dioxygenase (TauD). 牛磺酸双加氧酶的系统发育研究。

IF 2.6 3区生物学

Current Microbiology Pub Date : 2025-09-26 DOI: 10.1007/s00284-025-04494-6

Malini Sundar Rajan, Tamilselvan Jayavelu, Gautam Pennathur

引用次数: 0

Long-Term Surveillance of Avian Coronaviruses from Wild Bird in South Korea. 韩国野鸟源禽冠状病毒的长期监测

IF 2.6 3区生物学

Current Microbiology Pub Date : 2025-09-26 DOI: 10.1007/s00284-025-04513-6

Chae-Eun Kim, Tran Bac Le, Eun-Kyo Hong, Jeong-Eun Kim, Sook-San Wong, Hae-Jin Cho, Dae Gwin Jeong, Zanin Mark, Sun-Woo Yoon

{"title":"Long-Term Surveillance of Avian Coronaviruses from Wild Bird in South Korea.","authors":"Chae-Eun Kim, Tran Bac Le, Eun-Kyo Hong, Jeong-Eun Kim, Sook-San Wong, Hae-Jin Cho, Dae Gwin Jeong, Zanin Mark, Sun-Woo Yoon","doi":"10.1007/s00284-025-04513-6","DOIUrl":"10.1007/s00284-025-04513-6","url":null,"abstract":"We examined 2,868 fecal samples from migratory wild birds collected from multiple geographical locations in South Korea during the winter seasons between 2014 and 2022. All samples were obtained from four avian species belonging to the genus Anas within the order Anseriformes: Eurasian teal (Anas crecca), Eastern spot-billed duck (Anas zonorhyncha), Mallard (Anas platyrhynchos), and Northern pintail (Anas acuta). The presence of coronaviruses (CoVs) was confirmed through nested PCR targeting a conserved region of the RNA-dependent RNA polymerase (RdRp) gene. A total of 28 samples (0.97%) were tested positive for CoVs, comprising 17 Gamma coronaviruses (γ-CoVs) and 11 Delta coronaviruses (δ-CoVs). Sequence analysis of newly identified strains showed similarity ranging from 79.2% to 99.4% for γ-CoVs and from 72.5% to 100% for δ-CoVs, with an overall similarity of 74%. However, sequence homology between γ-CoVs and δ-CoVs was below 60%. The detected γ-CoVs belonged to two subgenera: Igacovirus (Duck-CoV 2714-like) and Brangacovirus. The δ-CoVs were classified under the Buldecovirus subgenus. Among them, seven detected strains were related to the representative porcine δ-CoV strain HKU15, while four strains clustered within subgroup 1 of Buldecovirus. Delta coronaviruses have been found in a variety of hosts, including humans and mammals, raising concerns about potential interspecies transmission. Evidence of genetic linkage between avian and porcine δ-CoVs was observed. Notably, recent reports indicate that three Haitian children were infected with porcine δ-CoVs. This study aimed to investigate the diversity of CoVs in avian species, and for the first time in South Korea, we report the detection of δ-CoVs in wild birds.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 11","pages":"524"},"PeriodicalIF":2.6,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474655/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145148252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a Genetically Modified Lactococcus lactis Strain that Produces a Single-Chain Variable Fragment Targeting Interleukin-6 Receptor α to Suppress Serum Amyloid A. 一株靶向白细胞介素-6受体α单链可变片段抑制血清淀粉样蛋白a的转基因乳酸乳球菌的培养

IF 2.6 3区生物学

Current Microbiology Pub Date : 2025-09-26 DOI: 10.1007/s00284-025-04518-1

Masahiro Yoda, Kaho Suzuki, Shoko Yoda, Kazuma Inoue, Mao Kagotani, Misato Kubo, Aito Murakami, Fu Namai, Takashi Sato, Takeshi Shimosato

{"title":"Development of a Genetically Modified Lactococcus lactis Strain that Produces a Single-Chain Variable Fragment Targeting Interleukin-6 Receptor α to Suppress Serum Amyloid A.","authors":"Masahiro Yoda, Kaho Suzuki, Shoko Yoda, Kazuma Inoue, Mao Kagotani, Misato Kubo, Aito Murakami, Fu Namai, Takashi Sato, Takeshi Shimosato","doi":"10.1007/s00284-025-04518-1","DOIUrl":"10.1007/s00284-025-04518-1","url":null,"abstract":"Interleukin-6 (IL-6) is a pivotal cytokine in immune regulation, inflammatory responses, and chronic inflammation. Dysregulated IL-6 signaling, which results from the interaction between IL-6 and IL-6 receptor alpha (IL-6Rα), has been implicated in the pathogenesis of numerous inflammatory diseases. Although anti-IL-6Rα monoclonal antibodies have been used to treat these diseases, their high treatment costs impose a substantial financial burden on patients. Recently, a genetically engineered strain of Lactococcus lactis (gmLAB) has attracted attention as a low-cost therapeutic agent. Thus, in this study, as a lower-cost alternative, a novel strategy using a gmLAB to produce a single-chain variable fragment targeting IL-6Rα (IL-6RαscFv) was developed. A recombinant strain that produces IL-6RαscFv with high binding affinity for human IL-6Rα, effectively inhibiting the interaction between IL-6 and IL-6Rα, was successfully produced. Furthermore, IL-6RαscFv significantly suppressed the expression of serum amyloid A, a biomarker of acute-phase inflammation. These findings demonstrate the potential of the recombinant strain as a promising therapeutic approach for inflammatory diseases.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 11","pages":"522"},"PeriodicalIF":2.6,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474681/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145148243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0