Current Microbiology最新文献_第4页

Chlorhexidine-Loaded Zinc Nanoparticles: A Potent Antibacterial Agent Against Streptococcus pneumoniae. 载洗必泰锌纳米颗粒：抗肺炎链球菌的有效抗菌剂。

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-05-30 DOI: 10.1007/s00284-025-04290-2

Rakesh Kumar, Renu Sharma, Sushila Kaura, Neeraj Sethi, Ikbal Shah, Kumar D Gahlot

{"title":"Chlorhexidine-Loaded Zinc Nanoparticles: A Potent Antibacterial Agent Against Streptococcus pneumoniae.","authors":"Rakesh Kumar, Renu Sharma, Sushila Kaura, Neeraj Sethi, Ikbal Shah, Kumar D Gahlot","doi":"10.1007/s00284-025-04290-2","DOIUrl":"10.1007/s00284-025-04290-2","url":null,"abstract":"Nanoformulations deliver antibacterial agents synergistically. Positively charged Zn nanocomplexes were used as carriers for chlorhexidine (CHX), developed using ionic liquids. The CHX-loaded Zn nanoparticles (CHZNPs) were characterised through various techniques, including UV-visible Spectroscopy, TEM, FTIR, and Zeta potential analysis. The average diameters of ZNPs and CHZNPs were 27.43 and 29.66 nm, respectively. CHZNPs consistently released CHX, enhancing its antibacterial effect. Tests against antibiotic-resistant Streptococcus pneumoniae strain 7465 revealed that CHZNPs significantly reduced bacterial viability. At 100 μg/mL, CHX showed the highest antibacterial activity with the lowest minimal inhibitory concentration (MIC90) and minimal bactericidal concentration (MBC96) values, followed by CHZNPs, which had lower MIC and MBC values. While ZNPs demonstrated some bactericidal effect at intermediate dosages (12 and 25 μg/mL), they could not fully inhibit bacterial growth. CHZNPs outperformed ZNPs across all concentrations, with an MIC of 40 μg/mL compared to CHX's 80 μg/mL. ZNPs showed no MIC at tested concentrations. Overall, CHZNPs significantly reduced bacterial viability more effectively than CHX alone, highlighting their potential as a treatment for antibiotic-resistant S. pneumoniae infections.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"316"},"PeriodicalIF":2.3,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12125063/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial Selenization as a Strategy to Deliver Selenium and Selenium Nanoparticles in Mango-Passion Fruit Juice Fermented by Indigenous Lactic Acid Bacteria. 本地乳酸菌发酵芒果-百香果汁中硒和纳米硒的微生物硒化策略

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-05-30 DOI: 10.1007/s00284-025-04255-5

Laura Crespo, Franco Darío Della Fontana, Brenda Sede Lucena, Agustina Marcela Lotufo Haddad, María Cristina Goldner, Fernanda Mozzi, Micaela Pescuma

{"title":"Microbial Selenization as a Strategy to Deliver Selenium and Selenium Nanoparticles in Mango-Passion Fruit Juice Fermented by Indigenous Lactic Acid Bacteria.","authors":"Laura Crespo, Franco Darío Della Fontana, Brenda Sede Lucena, Agustina Marcela Lotufo Haddad, María Cristina Goldner, Fernanda Mozzi, Micaela Pescuma","doi":"10.1007/s00284-025-04255-5","DOIUrl":"10.1007/s00284-025-04255-5","url":null,"abstract":"This study explores microbial selenization as a strategy for delivering selenium (Se) and seleno-nanoparticles (SeNPs) in a mango and passion fruit juice fermented by indigenous lactic acid bacteria (LAB). The fruit juice was fermented by a mixed culture of Se-enriched cells of Fructobacillus tropaeoli CRL2034 and Lactiplantibacillus paraplantarum CRL2051 at 30 °C for 24 h. The fermented beverages' intracellular Se and SeNPs content, antioxidant activity, microbial ability to survive gastrointestinal conditions, and sensory properties were evaluated. The mixed starter culture grew 2.04 ± 0.17 log CFU/mL in the fruit juice after 24 h and produced lactic acid, acetic acid, and mannitol. After gastrointestinal digestion, the fermented juice exhibited high antioxidant activity (14.20 ± 0.19 µM TE/mL) and a good survival rate (8.29 ± 0.06 log CFU/mL). The SeNPs were detected after digestion by scanning electron microscopy. The fermented and non-fermented juices were accepted by the consumers indiscriminately and their consumption intention was similar (41.7% and 32.5%, respectively). The juices had a sensory shelf life of 30 days. An increase in the Se concentration (from 20.64 to 68.27 µg Se/L, P < 0.05) in the fermented juice using selenized cells was observed. This beverage provides 40% of the recommended daily Se intake in a 200 mL serving. Combining Se and fermentation by rationally selected LAB offers a promising strategy for enhancing fruit-based beverages' nutritional value and stability. This fermented juice could be used as a dietary supplement of Se, and as a vehicle to deliver potentially probiotic bacteria.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"318"},"PeriodicalIF":2.3,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Different Expression of Nuclear Respiratory Factor 1 (NRF-1) Gene in COVID-19: An Insight into Disease Severity. 核呼吸因子1 （NRF-1）基因在COVID-19中的不同表达：对疾病严重程度的认识

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-05-30 DOI: 10.1007/s00284-025-04296-w

Shahrzad Shoraka, Seyed Reza Mohebbi, Seyed Masoud Hosseini, Asma Moradi, Shabnam Shahrokh, Amir Sadeghi, Mohammad Reza Zali

{"title":"Different Expression of Nuclear Respiratory Factor 1 (NRF-1) Gene in COVID-19: An Insight into Disease Severity.","authors":"Shahrzad Shoraka, Seyed Reza Mohebbi, Seyed Masoud Hosseini, Asma Moradi, Shabnam Shahrokh, Amir Sadeghi, Mohammad Reza Zali","doi":"10.1007/s00284-025-04296-w","DOIUrl":"10.1007/s00284-025-04296-w","url":null,"abstract":"Mitochondrial quality control is carried out through mechanisms such as mitochondrial biogenesis, dynamics, and mitophagy. Recent studies have shown that SARS-CoV-2 can directly modulate mitochondrial biogenesis. Nuclear respiratory factor 1 (NRF-1) is an important mediator of genes involved in mitochondrial biogenesis. A key role of NRF-1 in innate antiviral immunity and a link between innate immunity and mitochondrial quality control has been suggested. This study aims to investigate the NRF-1 levels in COVID-19 patients and compare between asymptomatic and symptomatic cases. Also, the diagnostic values of NRF-1 in SARS-CoV-2 infection have been evaluated based on disease severity subgroups. Buffy coat samples were collected from 37 COVID-19 patients and 33 healthy individuals. The patient group was divided into the following subgroups: asymptomatic (n = 17), mild/moderate (n = 12), and severe (n = 8) based on clinical and laboratory parameters. After RNA extraction and cDNA synthesis, real-time PCR was used to determine relative expression levels of NRF-1. Our results showed that the NRF-1 levels were significantly higher in COVID-19 patients than in healthy individuals. Also, NRF-1 levels were increased in symptomatic, mild/moderate, and severe cases compared to asymptomatic COVID-19 patients. In addition, the results of ROC curve analysis showed that the level of NRF-1 has high discriminative power to differentiate between COVID-19 severity subgroups. NRF-1 mRNA levels are a promising biomarker for the COVID-19 severity. Further understanding of the role of NRF-1 and mitochondrial quality control in disease severity, and outcome of SARS-CoV-2 infection may help in COVID-19 managements.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"317"},"PeriodicalIF":2.3,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomic Response Pathways of Yeast to Crucial Polyphenolic Acids in Rosmarinus Acid Biosynthesis. 迷迭香酸生物合成中酵母对关键多酚酸的转录组反应途径

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-05-30 DOI: 10.1007/s00284-025-04286-y

Tao Xu, Jun-Lu Duan, Samuel Ntakirutimana, Li Wang, Zhi-Hua Liu, Chun Li, Bing-Zhi Li

{"title":"Transcriptomic Response Pathways of Yeast to Crucial Polyphenolic Acids in Rosmarinus Acid Biosynthesis.","authors":"Tao Xu, Jun-Lu Duan, Samuel Ntakirutimana, Li Wang, Zhi-Hua Liu, Chun Li, Bing-Zhi Li","doi":"10.1007/s00284-025-04286-y","DOIUrl":"10.1007/s00284-025-04286-y","url":null,"abstract":"Rosmarinic acid (RA) is a natural polyphenolic compound with various biological and pharmaceutical activities. Due to the limited availability in plant sources and the complexity of its chemical synthesis, the biosynthesis of RA shows great potential. However, few studies have investigated RA biosynthesis in S. cerevisiae, and inhibitory effects from its biosynthesis on intrinsic metabolic pathways remain unclear. In this study, RNA-seq technology was employed to investigate the stress response of S. cerevisiae to RA and its precursors (caffeic acid, CA and salvianic acid, SAA). The results revealed an increased inhibitory effect with a variation number of differentially expressed genes (DEGs): 338 for 200-mg/L SAA, 510 for 200-mg/L CA, and 934 for 200-mg/L RA. Furthermore, trends analysis of DEGs expression level uncovered similar stress response patterns of S. cerevisiae under RA and SAA, indicating a shared inhibition mechanism. Common response pathways, including ribosome biogenesis, RNA polymerase biosynthesis, and purine and pyrimidine metabolism, were elucidated. Additionally, common regulated genes (HSP12, PAU4, TIR3) and different regulated genes (UTP6, NAN1, IMP4) in aforementioned pathways were identified. Unique genes and pathways were also mapped to reveal the special response mechanism to different polyphenolic acids, such as oxidative phosphorylation for RA and amino acid metabolism for CA. Overall, this work provides a foundation for understanding transcriptomic response of yeast to RA and RA biosynthesis.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"315"},"PeriodicalIF":2.3,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current Progress and Future Perspective of Chlamydia trachomatis Infection: A Rising Threat to Women Health. 沙眼衣原体感染：对妇女健康日益严重的威胁的研究进展及未来展望。

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-05-29 DOI: 10.1007/s00284-025-04287-x

Vineeta Sharma, Mohd Mabood Khan

{"title":"Current Progress and Future Perspective of Chlamydia trachomatis Infection: A Rising Threat to Women Health.","authors":"Vineeta Sharma, Mohd Mabood Khan","doi":"10.1007/s00284-025-04287-x","DOIUrl":"10.1007/s00284-025-04287-x","url":null,"abstract":"The most common sexually transmitted infection (STI) is caused by intracellular obligate bacteria Chlamydia trachomatis (CT) globally. Many Chlamydia infected women are asymptomatic or only experience minor signs, but others go on to develop salpingitis, endometritis, pelvic inflammatory disease (PID), ectopic pregnancy, and tubal factor infertility. It's been linked to both the spread of HIV and new infections, and it's suspected of playing a role in the emergence of cervical cancer. Clinically applicable rapid approaches for timely and precise identification of C. trachomatis infection would provide early treatment, hence reducing the risk of consequences. Traditional diagnostic methods Cell culture, direct immunofluorescence etc. are time-consuming but very sensitive assays for C. trachomatis infection. New and fast diagnostic method includes extraordinary optical transmission (EOT), loop-mediated isothermal amplification (LAMP), recombinase polymerase amplification (RPA), and microwave-accelerated metal-enhanced fluorescence (MAMEF) is more simple, rapid, sensitive, and cost-saving. This review provides fresh insight into our current understanding of C. trachomatis infection with emphasis on women's health. We outline a crucial summary of risk factors, molecular and clinical picture, prevention, diagnosis of C. trachomatis infection. Further, this review provides various modes of treatment, including available vaccination against genital C. trachomatis infection and the future directions to combat this sexually transmitted infection.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"314"},"PeriodicalIF":2.3,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144179735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proposal to Transfer Bacillus yapensis to the Genus Robertmurraya as Robertmurraya yapensis comb. nov. 将芽孢杆菌（Bacillus yapensis）转移到Robertmurraya属作为Robertmurraya yapensis梳子的建议。11月。

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-05-28 DOI: 10.1007/s00284-025-04292-0

Zhouyan Dong, Jiangshan Hou, Syed Raziuddin Quadri, Ngoc Tung Quach, Manik Prabhu Narsing Rao, Yulong Wu

引用次数: 0

Salt-induced Reduction of Hyperswarming Motility in Bacillus cereus MHS is Associated with Reduction in Flagellation, Nanotube Formation and Quorum Sensing Regulator plcR. 盐诱导蜡样芽孢杆菌MHS的过热运动性降低与鞭毛、纳米管形成和群体感应调节因子plcR的减少有关。

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-05-28 DOI: 10.1007/s00284-025-04288-w

Nirbhay K Bhadani, Kritika Prasad, Nisha Gupta, Hemanta Sarmah, Tapas K Sengupta

{"title":"Salt-induced Reduction of Hyperswarming Motility in Bacillus cereus MHS is Associated with Reduction in Flagellation, Nanotube Formation and Quorum Sensing Regulator plcR.","authors":"Nirbhay K Bhadani, Kritika Prasad, Nisha Gupta, Hemanta Sarmah, Tapas K Sengupta","doi":"10.1007/s00284-025-04288-w","DOIUrl":"10.1007/s00284-025-04288-w","url":null,"abstract":"Bacteria have been known to thrive in challenging environmental niches through diverse phenomena. Swarming is one such favourable adaptations that could help bacteria survive extreme conditions. Therefore, targeting swarming is crucial for improving our understanding of bacterial motility and preventing related infections. Bacillus cereus, which causes food poisoning, has been shown to perform swarming, and salts like NaCl can act as a food preservative to control bacterial growth. To explore the possible alterations in the swarming of Bacillus cereus in the presence of salt, the present study encompasses the effect of NaCl on the swarming characteristics of a natural bacterial isolate, Bacillus cereus MHS, with a hyperswarming phenotype. Here we report that increased NaCl in growth media could induce a reduction in swarming motility and pattern of MHS on Luria agar plates. This observed reduction in swarming was found to be associated with reduced flagellation and a reduction in the abundance of bacterial nanotubes. Gene expression studies supported the phenotypic and ultrastructure observations as the expressions of bfla and ymdB genes, involved in formations of flagella and nanotubes, respectively, were found to be reduced in the swarming MHS cells in the presence of increased NaCl. It was also observed that the salt-induced reduction in swarming of MHS is associated with the reduced expression of the quorum sensing regulator gene plcR. This study first time reports the bacterial nanotubes in a Bacillus cereus strain indicating a possible link between the bacterial nanotube formation and hyperswarming phenotype in Bacillus cereus MHS.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"313"},"PeriodicalIF":2.3,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144173074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacteria in the Phylum Bacillota Degrade Nicotine Through the Variant of Pyridine and Pyrrolidine Pathway. 杆菌门细菌通过吡啶和吡咯烷途径的变异降解尼古丁。

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-05-27 DOI: 10.1007/s00284-025-04270-6

Xiu-Ming Liu, Xuan Hai, Cong-Jian Li, Yu-Fan Han, Xing Chen, Yu-Jin Fan, Jian Wu, En-Min Zhou, Yuan-Dong Li, Ling-Ling Yang, Yan-Qing Duan, Xiao-Yang Zhi

{"title":"Bacteria in the Phylum Bacillota Degrade Nicotine Through the Variant of Pyridine and Pyrrolidine Pathway.","authors":"Xiu-Ming Liu, Xuan Hai, Cong-Jian Li, Yu-Fan Han, Xing Chen, Yu-Jin Fan, Jian Wu, En-Min Zhou, Yuan-Dong Li, Ling-Ling Yang, Yan-Qing Duan, Xiao-Yang Zhi","doi":"10.1007/s00284-025-04270-6","DOIUrl":"10.1007/s00284-025-04270-6","url":null,"abstract":"Nicotine is a harmful contaminant primarily derived from the waste produced by tobacco manufacturing facilities. Many bacterial strains have been reported to degrade nicotine, indicating their potential application in removing nicotine from sewage for wastewater treatment. The microbial degradation of nicotine is a green, economical, and efficient strategy, leading to a significant interest in discovering novel nicotine-degrading bacterial isolates. In this study, we isolated and identified 192 strains from tobacco leaves, roots, and rhizosphere soil. Nicotine utilization assays showed that 64 out of 192 strains had the potential capability of nicotine degradation, primarily within the genera Bacillus, Peribacillus, and Priestia in phylum Bacillota. Comparative genome analysis identified genes related to the variant of pyridine and pyrrolidine pathway scattered throughout the genomes of novel Bacillota strains. Phylogenetic analysis indicated that essential genes from these strains were evolutionarily distinct from others, forming a monophyletic clade. Our observations revealed that nicotine degradation-related genes have relatively independent origins and complex evolutionary histories in the phylum Bacillota.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"310"},"PeriodicalIF":2.3,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144149662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Testing the Effectiveness of DNA Barcoding Markers and Species Delimitation Methods Within the Genus Coelastrella (Sphaeropleales, Chlorophyta), With a Description of Coelastrella polaris sp. nov. Isolated from Arctic Soils. 蓝藻属（Sphaeropleales，绿藻门）DNA条形码标记和物种划分方法的有效性检验——以北极土壤中分离的北极蓝藻为例。

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-05-27 DOI: 10.1007/s00284-025-04293-z

E Krivina, M Sinetova, A Starikov, O Anissimova, A Temraleeva

{"title":"Testing the Effectiveness of DNA Barcoding Markers and Species Delimitation Methods Within the Genus Coelastrella (Sphaeropleales, Chlorophyta), With a Description of Coelastrella polaris sp. nov. Isolated from Arctic Soils.","authors":"E Krivina, M Sinetova, A Starikov, O Anissimova, A Temraleeva","doi":"10.1007/s00284-025-04293-z","DOIUrl":"10.1007/s00284-025-04293-z","url":null,"abstract":"The species diversity in the genus Coelastrella is not yet fully unclarified, particularly with regard to algal communities in ecosystems characterized by extreme climatic conditions, such as those found in polar regions. The study objects were strains VKM Al-421, VKM Al-488, and VKM Al-489 isolated from the soils of the Far North, Russian Federation. The analysis of the 18S-ITS1-5.8S-ITS2 fragment revealed that these strains represent a unique phylogenetic lineage outside the 'core Coelastrella' group. The species status is also confirmed by morphological differences between the studied species and its sister species (absence of edges) (lack of ribs), interspecific genetic distances, the presence of compensatory base changes in the internal transcribed spacers ITS1 and ITS2, as well as the results of delimitation using various algorithms applied to both the full-length fragment and shorter barcodes. The strain VKM Al-421 of the new species exhibits a fatty acid profile characteristic of the genus Coelastrella; however, it differs from closely related strains due to the presence of Δ7,10,13-hexadecatrienoic acid and a notably high concentration of α-linolenic acid. These features may indicate an adaptation to polar regions. In addition, the studied strain has the potential to be used as a producer of α-linolenic acid, which is essential for human health. A detailed comparative analysis of the effectiveness of different DNA barcodes showed that ITS2 is the most promising for distinguishing species within Coelastrella. Among all species delimitation algorithms, GMYC is the most accurate when working with variable barcodes. At the same time, the less laborious KoT algorithm demonstrated a similar level of accuracy for ITS1.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"311"},"PeriodicalIF":2.3,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144149688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyphasic Taxonomic Analysis of Pontitalea aquivivens gen. nov., sp. nov., Isolated from Seawater. 海水中水生Pontitalea aquvivens gen. nov., sp. nov.的多相分类分析。

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-05-27 DOI: 10.1007/s00284-025-04285-z

Jaewoo Yoon

{"title":"Polyphasic Taxonomic Analysis of Pontitalea aquivivens gen. nov., sp. nov., Isolated from Seawater.","authors":"Jaewoo Yoon","doi":"10.1007/s00284-025-04285-z","DOIUrl":"10.1007/s00284-025-04285-z","url":null,"abstract":"A Gram-stain-negative, rod-shaped, strictly aerobic, non-motile, and chemo-organoheterotrophic alphaproteobacterium, designated KMU-169T, was isolated from coastal seawater in the Republic of Korea. The novel isolate was able to grow at 0-2.0% NaCl concentrations (w/v), pH 6.0-9.5, and 15-40 °C. The analysis based on 16S rRNA gene sequences indicated that strain KMU-169T belongs to the family Paracoccaceae and shared the highest similarity (97.0%) with \"Defluviimonas salinarum\" CAU 1641T. The major (> 10%) cellular fatty acids were C18:1 ω7c and summed feature 3 (comprising C16:1 ω6c and/or C16:1 ω7c). The predominant respiratory quinone was ubiquinone-10. Strain KMU-169T comprised phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, an unidentified aminolipid, an unidentified phospholipid, and an unidentified lipid. The assembled draft genome size of strain KMU-169T was 4.1 Mbp with a DNA G + C content of 65.0%. The average nucleotide identity (ANI), average amino acid identity (AAI), and digital DNA-DNA hybridization (dDDH) values between the genomes of strain KMU-169T and its closely related taxa were 71.9-76.8%, 60.9-76.1%, and 18.4-21.0%, respectively. The genome of the strain KMU-169T revealed the presence of numerous genes involved in bio-macromolecule degradation, indicating a high potential for producing industrially valuable enzymes. Based on the polyphasic taxonomic data reported in this study, a novel genus and a new species of the family Paracoccaceae, for which the name Pontitalea aquivivens gen. nov., sp. nov., is proposed with the type strain KMU-169T (= KCCM 90598T = NBRC 117093T).","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"308"},"PeriodicalIF":2.3,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144149683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0