Current Microbiology最新文献

{"title":"Nature-Inspired Antimicrobial Agents: Cinnamon-Derived Copper Oxide Nanoparticles for Effective Aspergillus Niger Control.","authors":"Zunaira Iram, Asma Irshad, Alexis Spalletta, Rabbia Jawad, Qudsia Mushtaq, Nicolas Joly, Patrick Martin, Uzair Ishtiaq","doi":"10.1007/s00284-024-04000-4","DOIUrl":"https://doi.org/10.1007/s00284-024-04000-4","url":null,"abstract":"<p><p>The emergence of resistant bacterial and fungal strains poses significant challenges in various industrial processes including food, medicines, and leather industry. It necessitates the development of novel and effective antimicrobial agents. In the present work, we have developed an ecofriendly and sustainable approach to synthesize silver-doped copper oxide nanoparticles by using cinnamon bark extract (C-CuO/Ag). The nanoparticles were characterized via UV-visible spectroscopy at 190-800 nm, FT-IR, SEM-EDAX, XRD, and further subjected to determine antimicrobial potential, minimum inhibitory concentration (MIC), and anti-biofilm potential against both bacterial and fungal species. UV-visible spectrum showed maximum absorption at 210 nm in ultraviolet range and 419 nm in visible range. Various strong and weak peaks were obtained in FT-IR spectra, which defined the presence of corresponding functional groups in C-CuO/Ag nanoparticles. SEM analysis revealed the tightly packed confirmation, while EDS confirmed the elemental analysis of C-CuO/Ag nanoparticles. XRD spectrum of C-CuO/Ag nanoparticles showed strong diffraction peaks at 2Ө of 31.92˚, 35.67˚, and 48.51˚, which confined with the plane indices of (-110), (111), and (- 202), respectively, while weak diffraction peaks at 2Ө of 56.31˚, 58.9˚, and 77.4˚, which leads to the crystal planes of (202), (- 220), and (311), respectively. Antimicrobial assays showed clear zones of inhibition against microbial strains as maximum inhibition diameter was observed against Aspergillus niger (31.5 ± 0.7 mm) followed by Escherichia coli (30.1 ± 0.3 mm) and Staphylococcus aureus (29.5 ± 0.7 mm). These findings provide support clear evidence that CuO nanoparticles can serve as potent antibacterial and antifungal compounds against highly resistive and pathogenic microbial strains.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 1","pages":"19"},"PeriodicalIF":2.3,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142750357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PA5402-5407 of Pseudomonas aeruginosa Regulate the Expression of the Aa3-Type Oxidases and Their Growth Under Carbon Starvation and High-Density Conditions.","authors":"Haiyu Sun, Shenping Wang, Shuheng Du, Na Wang, Runqing Shi, Kairui Zhao, Chenghao Huang, Yaodong Chen","doi":"10.1007/s00284-024-03984-3","DOIUrl":"https://doi.org/10.1007/s00284-024-03984-3","url":null,"abstract":"<p><p>Our previous studies identified PA5407 in Pseudomonas aeruginosa as a new regulatory protein for bacterial division and named it ZapAL. This protein enhances the assembly of the key bacterial division protein FtsZ and participates in the assembly of the bacterial Z-ring, but its physiological function is not clear. ZapAL is in the same gene cluster as PA5402-5406, and in this study, we found that these genes are involved in the regulation of bacterial growth under nutrient deficiency and high-density conditions. The expression of Aa3 oxidase increases significantly at the end of the stationary phase of bacterial growth under aerobic conditions, and appropriately accelerate energy intake to adapt to the adversity. In our study, we found that the knockout of PA5402-5407 in P. aeruginosa promotes the further expression of Aa3 oxidase and its expression increased more and faster than the wild type, especially under carbon starvation and high-density conditions. This results in a larger bacteria population, but the average length of the bacteria is abnormally reduced. In summary, our study found that the P. aeruginosa gene cluster PA5402-5407 regulated the expression of Aa3 oxidase in the late stationary phase, and these genes balance energy intake, growth and division of bacteria under adverse conditions.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 1","pages":"18"},"PeriodicalIF":2.3,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142738568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Repurposed Anti-Multiple Sclerosis Drug Fty720 Targets Carbapenem-Resistant Acinetobacter baumannii via Multiple Pathways.","authors":"Yuxuan Wu, Yufan Pang, Han Yang, Li Zhu, Tonghui Ma, Xiuli Chen","doi":"10.1007/s00284-024-03986-1","DOIUrl":"https://doi.org/10.1007/s00284-024-03986-1","url":null,"abstract":"<p><p>Bacterial antimicrobial resistance (AMR), particularly multidrug resistance (MDR) in gram-negative bacterial strains, has emerged as a formidable challenge of substantial consequence, necessitating an urgent pursuit of a sustainable and efficacious strategic response. Repurposing nonantibiotic drugs as potential antibiotics or antibiotic adjuvants is a valuable approach to targeting MDR bacteria. A total of 1,750 FDA-approved drugs (APExBIO, USA) were screened to test their antimicrobial activities against MDR bacteria using the broth microdilution method according to the standard of the Clinical and Laboratory Standards Institute (CLSI). Microscale thermophoresis (MST) analysis was performed to detect the Fty720-LPS interactions. Fty720-indcued lipid changes were measured by untargeted lipidomic analysis. Isothermal titration calorimetry (ITC) analysis was used to determine the Fty720-lipid binding affinities. DNA degradation was assessed via agarose gel electrophoresis with ethidium bromide (EB) staining and visualized using a gel imaging system. Galleria mellonella larvae infection model and Mouse peritonitis infection models were used to evaluated the antibacterial ability of Fty720 in vivo. In this study, we identified Fty720, a pharmaceutical agent for treating multiple sclerosis, as a potent inhibitor of carbapenem-resistant Acinetobacter baumannii (CRAB). We demonstrated that Fty720 exerts antibacterial effects through multiple strategies, including disruption of the structural integrity of the membranes by interacting with LPS and glycerophospholipids, as well as degradation of bacterial DNA. Furthermore, through judicious structural modification, the pivotal role of the positively charged moiety (NH2) in Fty720's antibacterial activity was substantiated. Intriguingly, the translation of Fty720's antibacterial efficacy was demonstrated in vivo, substantiating its pronounced influence on elevating survival rates among models afflicted with MDR gram-negative bacterial infections. Fty720 targets CRAB via multiple pathways, including disruption of outer and inner membrane integrity and DNA degradation. This investigation unveils the multifaceted antibacterial mechanisms of Fty720 while concurrently delineating a prospective therapeutic avenue to counteract MDR gram-negative bacterial strains.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 1","pages":"17"},"PeriodicalIF":2.3,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142738571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Detection of Vibrio parahaemolyticus in Migratory Birds in Egypt: Antibiogram, Virulence, and Resistance Gene Profiles Indicating Zoonotic and Public Health Risks.","authors":"Sherief M Abdel-Raheem, Saad Ibrahim Al-Sultan, Reham M El-Tarabili","doi":"10.1007/s00284-024-03992-3","DOIUrl":"https://doi.org/10.1007/s00284-024-03992-3","url":null,"abstract":"<p><p>This study examined the occurrence of Vibrio parahaemolyticus obtained from migrating birds, marking the first instance of such research conducted in Egypt. The study assessed potential risks using an antibiogram, virulence characteristics, antibiotic-resistance, and gene profile. Randomly collected 80 samples were tested for V. parahaemolyticus. Eleven (13.75%) samples were V. parahaemolyticus-contaminated. All isolates were positive for 16SrRNA and species-specific toxR genes. Interestingly, our strain is genetically similar to human and shrimp isolates, suggesting zoonotic transmission may pose a health danger. All isolates had 100% l-lysine decarboxylase, 45.45% beta-hemolytic, and 100% l-ornithine decarboxylase activity. All isolates displayed no l-arginine decarboxylase activity. Notably, every isolate possessed a minimum of two virulence genes. In addition, the profiles of virulence genes were identified, tdh + /trh + (27.3%), tdh-/trh + (27.3%), and tdh + /trh- (45.4%). Out of the V. parahaemolyticus isolates, 18.2% (2/11) were extensively drug-resistant (XDR) to six different antimicrobials classes and possessed the bla_TEM, bla_OXA, sul1, and tetA genes. Furthermore, 63.6% of the isolates displayed multidrug resistance (MDR). The correlation highlights a strong relationship between phenotypic and genotypic resistance. Besides the strong correlation between virulence and resistance genes. In summary, this work highlighted the presence of newly identified MDR and XDR V. parahaemolyticus carried toxR, trh, and tdh virulence genes, as well as bla_TEM, bla_OXA, sul1, and tetA resistance genes in migratory birds, indicating a significant public health risk.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 1","pages":"15"},"PeriodicalIF":2.3,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142727047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic Insights of Multidrug-Resistant Enterococcus faecalis and Acinetobacter baumannii Isolated from a Sepsis Patient with Pauci-Immune Crescentic Glomerulonephritis, India.","authors":"Ujjayni B Saha, Kunal K Dixit, Savita V Jadhav, Ketaki N Pathak, Neetu S Gupta, Sunil D Saroj","doi":"10.1007/s00284-024-04003-1","DOIUrl":"10.1007/s00284-024-04003-1","url":null,"abstract":"<p><p>Acinetobacter baumannii and Enterococcus faecalis are opportunistic bacteria frequently associated with hospital-acquired infections. A. baumannii nosocomial infections in intensive care units are a worldwide problem, with high mortality rates. It may also develop rapidly multidrug resistance (MDR), extensive drug resistance (XDR), and even pan-drug resistance (PDR). Colistin resistance which is an example of pan-drug resistance, is highly alarming as it's used as a last-line antibiotic. Microbes capable of crossing epithelial barriers such as E. faecalis have developed novel strategies to counter antimicrobial agents and cause bacteremia in immunocompromised patients. However, the coinfection of these bacteria in the same patient is unusual. Here, we report a genomic investigation of the extensively drug-resistant E. faecalis and A. baumannii isolated from the blood sample of a patient diagnosed with pauci-immune crescentic glomerulonephritis (PICGN). Identification of cultures isolated from blood sample was carried out using whole-genome sequencing and resistome profiles were mapped. Whole genome sequencing revealed that E. faecalis SVJ-EF01 had a genome size of 2,935,226 bp and GC content of 37.4%, whereas A. baumannii SVJ-AC01 had a genome size of 3,730,857 bp and GC content of 39%. Draft genomes were functionally annotated demonstrating that the organism harbors multiple virulence factors and antimicrobial-resistant mechanisms including MDR efflux pumps. A. baumannii genome possessed a CRISPR-Cas system which might contribute to antimicrobial resistance. This highlights the significance of polymicrobial nature in ESKAPE pathogenesis research. This genomic investigation helps to gain insights into the virulence, resistance profile, and functional potential of these pathogens.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 1","pages":"16"},"PeriodicalIF":2.3,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142738615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent Advances in Gut Microbiome Modulation: Effect of Probiotics, Prebiotics, Synbiotics, and Postbiotics in Inflammatory Bowel Disease Prevention and Treatment.","authors":"Samira Saedi, Safoura Derakhshan, Alka Hasani, Manouchehr Khoshbaten, Vahdat Poortahmasebi, Payam Gonbari Milani, Javid Sadeghi","doi":"10.1007/s00284-024-03997-y","DOIUrl":"10.1007/s00284-024-03997-y","url":null,"abstract":"<p><p>The human gastrointestinal tract contains trillions of microbes that affect the body. Dysbiosis in the composition of gut microbiota is one of the leading causes of chronic inflammatory diseases such as inflammatory bowel disease (IBD). IBD is a global public health challenge and millions of people in the world are suffering from this disease. It is a recurring inflammatory disease that affects different parts of the human digestive system. Ulcerative colitis and Crohn's disease are the two main types of IBD with similar clinical symptoms. The increasing incidence and severity of IBD require new treatment methods. The composition of the gut microbiota can be modified using dietary supplements such as prebiotics and bacterial supplements called probiotics. Furthermore, the effects of the microbiome can be improved by using paraprobiotics (non-viable, inactivated bacteria or their components) and/or postbiotics (products of bacterial metabolism).</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 1","pages":"12"},"PeriodicalIF":2.3,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142715533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Altererythrobacter arenosus sp. nov., Isolated from Marine Sediment.","authors":"Soyeon Ahn, Yunjeong Lee, Veeraya Weerawongwiwat, Jong-Hwa Kim, Jung-Sook Lee, Jung-Hoon Yoon, Ampaitip Sukhoom, Wonyong Kim","doi":"10.1007/s00284-024-03995-0","DOIUrl":"https://doi.org/10.1007/s00284-024-03995-0","url":null,"abstract":"<p><p>Strain CAU 1644^T was isolated from sea sand at Ganghwa Island, Republic of Korea. The bacterium is aerobic, Gram-staining negative, yellow-colored, non-motile, catalase-positive, and rod-shaped. Cells of strain CAU 1644^T grew at 20-40 °C and pH 6.0-10.0 with 0%-3.0% (w/v) NaCl. The 16S rRNA gene sequences of Altererythrobacter epoxidivorans CGMCC 1.7731^T (identity, 98.5%) and A. xiamenensis CGMCC 1.12494^T (98.0%) had the highest similarities to strain CAU 1644^T. Strain CAU 1644^T exhibits affiliation with the genus Altererythrobacter by phylogenetic trees based on the 16S rRNA gene and core-genome sequences. The predominant quinone was ubiquinone-10 (Q-10) and the major fatty acids were summed feature 8 (C_18:1ω7c and/or C_18:1ω6c) and summed feature 3 (C_16:1ω7c and/or C_16:1ω6c). The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain CAU 1644^T were 77.6% and 20.1% with A. epoxidivorans CGMCC 1.7731^T, respectively, and 77.4% and 21.0% with A. xiamenensis CGMCC 1.12494^T. The G + C content is 62.5%. Based on phenotypic and genotypic analyses, strain CAU 1644^T represents a novel species of the genus Altererythrobacter, for which the name Altererythrobacter arenosus sp. nov., is proposed. The type strain is CAU 1644^T (= KCTC 92082^T = MCCC 1K07086^T).</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 1","pages":"13"},"PeriodicalIF":2.3,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142715531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Roseateles microcysteis sp. nov., Isolated from Co-cultivation of Microcystis aeruginosa and Myriophyllum spicatum.","authors":"Min-Seong Kim, Seonah Jeong, Mingyeong Kang, So-Ra Ko, Ve Van Le, Dong-Yun Choi, Chi-Yong Ahn","doi":"10.1007/s00284-024-04002-2","DOIUrl":"10.1007/s00284-024-04002-2","url":null,"abstract":"<p><p>A novel rod-shaped, Gram-stain-negative bacterial strain MS17^T was obtained from a co-culture of Microcystis aeruginosa and Myriophyllum spicatum. The examination of the 16S ribosomal RNA gene sequence showed a significant degree of similarity between strain MS17^T and Paucibacter sediminis S2-9^T (98.4%), Roseateles violae PFR6^T (98.1%), 'Roseateles cellulosilyticus' P8^T (98.0%), Roseateles aquae APW11^T (97.9%), Roseateles oligotrophus CHU3^T (97.7%), Roseateles saccharophilus DSM 654^T (97.6%), Kinneretia aquatilis CR182^T (97.7%), Pelomonas aquatica CCUG 52575^T (97.6%), and Roseateles toxinivorans 2C20^T (97.0%). Between strain MS17^T and the type strains of closely related species, digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values were below 21.9% and 78.48%. The respiratory quinone was ubiquinone Q-8. The main fatty acids (> 10.0%) were C_15:1 ω6c, C_16:0, C_17:0, and summed feature 3 (C_16:1 ω7c and/or C_16:1 ω6c). The polar lipids comprised phosphatidylethanolamine, unidentified phospholipid, phosphatidylglycerol, unidentified aminophospholipid, unidentified aminolipid, and three unidentified lipids. The genomic G + C content was 65.9%. According to phenotypic characteristics, phylogenetic relationships, and chemotaxonomic data, strain MS17^T has been categorized as a newly discovered species belonging to the Roseateles genus, and the name Roseateles microcysteis sp. nov. is suggested. It exhibits distinct biochemical properties that differentiate it from closely related species. The type strain is MS17^T (=KCTC 8001^T = LMG 33142^T).</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 1","pages":"11"},"PeriodicalIF":2.3,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142715535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The potency of Cupressus sempervirens and Eucalyptus globulus Essential Oils Against Antibiotic-Resistant Escherichia coli and Mammaliicoccus sciuri from Diseased Animals in Tunisia.","authors":"Sana Dhaouadi, Salma Kloula Ben Ghorbel, Wafa Bouglita, Soufiene Chaari, Wissal Dhifi, Rim Khrouf, Ameur Cherif, Ramzi Boubaker Elandoulsi","doi":"10.1007/s00284-024-03981-6","DOIUrl":"https://doi.org/10.1007/s00284-024-03981-6","url":null,"abstract":"<p><p>This study aimed to investigate the antibacterial activity of essential oils from Cupressus sempervirens (CSEO) and Eucalyptus globulus (EGEO) against clinical isolates of colistin-resistant E. coli and methicillin-resistant M. sciuri recovered from diseased food-producing animals in Tunisia. The chemical compositions of both essential oils were analyzed using Gas chromatography-mass spectrometry (GC-MS). Antibacterial activity was determined through disk diffusion and microdilution assays, while the fractional inhibitory concentration index (FICI) was used to measure the synergistic effect of CSEO and colistin. The major constituents of CSEO were α-pinene (59.03%), δ-3 Carene (21.48%), D-limonene (5.62%) and α-thujene (3.08%), while EGEO contained eucalyptol (52.1%), α-pinene (26.51%), γ-terpinene (4.81%) and D-limonene (3.54%). The inhibition zone diameters (IZDs) against E. coli and M. sciuri ranged from 6 to 29 mm for CSEO and 9.3 to 28.6 mm for EGEO. Minimum inhibitory concentrations (MICs) showed a range of 4 to 512 µg/mL for CSEO against M. sciuri and 2 to 426.66 µg/mL for EGEO. For E. coli, MICs ranged from 170.66 to 512 µg/mL for CSEO and 53.33 to 512 µg/mL for EGEO. The checkerboard test demonstrated a synergistic effect between CSEO and colistin against two colistin-resistant E. coli isolates, with FICI 3- to 32-fold lower than the MICs of the individual compounds. This study highlights the potency of essential oils from CSEO and EGEO against antibiotic-resistant M. sciuri and E. coli strains from animals in Tunisia and provides evidence of the synergistic effect between CSEO and colistin against clinically relevant colistin-resistant E. coli isolates.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 1","pages":"14"},"PeriodicalIF":2.3,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142727052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-omics Analysis of Klebsiella pneumoniae Revealed Opposing Effects of Rutin and Luteolin on Strain Growth.","authors":"Zhibin Wang, Wanxia Shen, Yuejiao Li, Xiaoyun Wang, Xiaolin Zhong, Xing Wang","doi":"10.1007/s00284-024-03982-5","DOIUrl":"https://doi.org/10.1007/s00284-024-03982-5","url":null,"abstract":"<p><p>The emergence of pathogenic bacteria resistant to conventional antibiotics is becoming increasingly challenging. Plant-derived flavonoids are potential alternatives to antibiotics, owing to their antimicrobial properties. However, the molecular mechanisms through which they inhibit the growth of pathogenic microorganisms remain unclear. Therefore, Klebsiella pneumoniae ATCC700603 was separately incubated in two flavonoids to elucidate their inhibitory mechanism. Metabolomic and transcriptomic analyses were performed after 4-h incubation. In total, 5483 genes and 882 metabolites were identified. Compared to the untreated control, rutin and luteolin activated 507 and 374 differentially expressed genes (DEGs), respectively. However, the number of differential abundant metabolites (DAMs) remained the same. The top 10 correlated DEGs and DAMs were identified within each comparative group after a correlation analysis. Rutin induced the accumulation of unique metabolites and suppressed gene expression whereas luteolin did not. Our results explain the disparate effects of these two flavonoids and demonstrate the inhibitory mechanism of rutin on strain growth.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 1","pages":"9"},"PeriodicalIF":2.3,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142709290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}