Current Microbiology最新文献

{"title":"Impact of Fermented Milk On Gut Microbiota And Human Health: A Comprehensive Review.","authors":"Mohamed H Abd El-Salam, Safinaz El-Shibiny, Fayza Mohamed Assem, Gharieb S El-Sayyad, Yasmeen A Hasanien, Dounia Elfadil, Tarek Nour Soliman","doi":"10.1007/s00284-025-04061-z","DOIUrl":"10.1007/s00284-025-04061-z","url":null,"abstract":"<p><p>The beneficial impact of gut microbiota on human health has encouraged studies on factors modulating it. Among the different factors, diet plays a vital role in this area. Many studies on animals and humans have been concerned with the effects of fermented milk products on gut microbiota and how they relate to health benefits. Yoghurt, kefir, Koumiss, and fermented kinds of milk made using different probiotic strains were tested for their capability to modulate gut microbiota. It is apparent that the microflora present in fermented milk, specifically probiotics, are capable of enduring the gastrointestinal tract's adverse conditions primarily through transit microorganisms. Meanwhile, they can alter the gut microbiota in several ways that benefit human health. The present article gives a comprehensive overview of the modulation of gut microbiota by consumption of fermented milk, particularly those containing probiotics, and their impact on human health.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 3","pages":"107"},"PeriodicalIF":2.3,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Paenibacillus mesotrionivorans sp. nov., a Mesotrione-Degrading Strain Isolated from Soil.","authors":"Ye Song, Yan Wu, Leyao Chen, Luyao Ruan, Minglai Wan, Bin Liu, Jian He, Baolong Zhang","doi":"10.1007/s00284-025-04086-4","DOIUrl":"10.1007/s00284-025-04086-4","url":null,"abstract":"<p><p>A Gram-stain-positive, facultatively anaerobic, motile with peritrichous flagella, and rod-shaped bacterium, designated as P15^T, was isolated from an agricultural soil sample collected in Jiangxi Province, PR China. Strain P15^T completely degraded 100 mg/L of mesotrione, a herbicide, within 48 h of incubation. Strain P15^T grew at 15-42 °C (optimum 30 °C), pH 6.0-9.0 (optimum 7.0), and 0-4.0% (w/v) NaCl (optimum 1.0%). Strain P15^T exhibited less than 93.3% 16S rRNA gene sequence similarity with type strains of genus Paenibacillus. In the phylogenetic tree based on 16S rRNA gene sequences, strain P15^T was clustered in genus Paenibacillus and formed a subclade with P. cavernae C4-5^T, P. contaminans CKOBP-6^T, and P. doosanensis CAU 1005^T. The major cellular fatty acids (≥ 5% of the total) were anteiso-C_15:0, iso-C_16:0, C_14:0, C_16:0, and iso-C_14:0. The predominant respiratory quinone was MK-7. The major polar lipids were diphosphatidylglycerol, phosphatidylglycero, phosphatidylethanolamine, one unidentified glycolipid, one unidentified aminophosphoglycolipid, two unidentified aminophospholipids, two unidentified phospholipid, and two unidentified lipids. The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid. The DNA G + C content was 53.9 mol%. Based on the phylogenetic, phenotypic, and chemotaxonomic characteristics, strain P15^T represents a novel species within genus Paenibacillus, for which the name Paenibacillus mesotrionivorans sp. nov is proposed, with strain P15^T (= MCCC 1K09191^T = KCTC 43705^T) as the type strain.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 3","pages":"108"},"PeriodicalIF":2.3,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advanced Peptide Nanozymes with Dual Antifungal Mechanisms: Cutting-Edge Innovations in Combatting Antimicrobial Resistance.","authors":"Ganeshraja Ayyakannu Sundaram","doi":"10.1007/s00284-025-04094-4","DOIUrl":"10.1007/s00284-025-04094-4","url":null,"abstract":"<p><p>This letter commends the recent innovative research findings on \"Dual-Action Antifungal Peptide Nanozymes: A Novel Approach to Combatting Antimicrobial Resistance.\" The study introduces a pioneering method to address antimicrobial resistance by developing peptide nanozymes that mimic antimicrobial peptides and enzymes through de novo design and peptide assembly. The heptapeptide IHIHICI, designed using AlphaFold2 and molecular dynamics simulations, exhibits high stability and dual antifungal actions, effectively killing over 90% of Candida albicans within 10 min. This research represents a significant advancement in the development of multifunctional antimicrobial materials, with promising biomedical applications.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 3","pages":"106"},"PeriodicalIF":2.3,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Crude Antibiotics and Antifungal Metabolites from Chaetomium globosum Cg6 Suppress Pythium aphanidermatum, Causal Agent of Rhizome Rot of Turmeric.","authors":"Jayaraman Rajalakshmi, Sankarasubramanian Harish, Lingan Rajendran, Seethapathy Parthasarathy, Kalaiselvan Saravanakumari, Thiruvengadam Raguchander","doi":"10.1007/s00284-025-04076-6","DOIUrl":"10.1007/s00284-025-04076-6","url":null,"abstract":"<p><p>Turmeric is affected by various phytopathogens, which cause huge economic losses to farmers. In the present study, ten isolates of Pythium spp. were isolated from infected turmeric rhizomes and characterized. Besides, forty-five isolates of Chaetomium spp. were isolated and characterized through morphological and molecular methods. Among the 45 isolates, Chaetomium globosum Cg6 exhibited the highest antagonistic activity against Pythium aphanidermatum in vitro. The crude antibiotics produced by C. globosum were characterized and analyzed through TLC, GC/MS, and HPLC. The best isolate, TNAU-Cg6, produced all three antibiotics, viz., chaetoglobosin A, chaetomin, and chaetocin in higher quantity and inhibited the growth of P. aphanidermatum. Liquid formulation of TNAU-Cg6 using modified Glucose Ammonium Nitrate broth supported the highest concentration at the end of 360 days at an optimum dosage of 10 mL l^-1. Besides, it survived as ascospores at 210 days after planting in the soil. It is observed that the sequential application of liquid bioformulated TNAU-Cg6 as rhizome dip and soil drenching significantly reduced the rhizome rot incidence and enhanced the plant growth parameters. Our findings suggested that C. globosum Cg6 can be a potential biocontrol agent against turmeric rhizome rot disease.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 3","pages":"105"},"PeriodicalIF":2.3,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From Farm to Community: Dispersal of Potentially Pathogenic Staphylococcus and Mammaliicoccus Species and Antimicrobial Resistance Across Shared Environments.","authors":"Faizan Ahmad, Samuel Sathler Martuchelle, Ana Luisa Andrade-Oliveira, Vitor Emanuel Lanes Viana, Maria Antônia Silva Melo Sousa, Felipe Sicchierolli da Silveira, Marisa Alves Nogueira-Diaz, Monalessa Fábia Pereira, Marcia Giambiagi-deMarval, Ciro César Rossi","doi":"10.1007/s00284-025-04079-3","DOIUrl":"10.1007/s00284-025-04079-3","url":null,"abstract":"<p><p>Staphylococcaceae are a diverse bacterial family with important implications for human and animal health. This study highlights the One Health relevance of their environmental dispersal, particularly, by identifying closely related or genetically identical strains circulating between farm and community environments. Environmental Staphylococcaceae strains were isolated from animal farms and interconnected areas within a university setting, both influenced by anthropogenic activities. Genetic diversity analysis via GTG₅-PCR revealed that specific strains circulated across these environments, likely facilitated by human interaction, supporting the potential for cross-environmental bacterial transfer. The strains were further tested for antimicrobial resistance against a panel of antibiotics, biofilm formation, and the presence of resistance-associated genes. We identified a wide array of Staphylococcaceae species (n = 16), with over half being pathogenic to humans and animals, including S. aureus, S. epidermidis, S. haemolyticus, and S. saprophyticus. Notably, 55.5% of the strains exhibited multidrug resistance, and 15.9% harbored the methicillin resistance gene mecA, underscoring the challenges these infections might pose to treatment. Biofilm-forming capabilities further enhance the environmental persistence of these strains. These findings underscore the critical need for surveillance and targeted intervention strategies in a One Health framework to mitigate antimicrobial resistance across shared environments.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 3","pages":"104"},"PeriodicalIF":2.3,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143058391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Identification of Apiospora intestini from Hedera nepalensis Leaf Blight and Determination of Antagonism of Phomopsis sp.","authors":"Jian Liu, Yajiao Sun, Mian Liu, Chaojin Gou, Huali Li, Fazhong Yang, Junjia Lu","doi":"10.1007/s00284-024-04016-w","DOIUrl":"10.1007/s00284-024-04016-w","url":null,"abstract":"<p><p>In order to identify the pathogen responsible for Hedera nepalensis leaf blight and investigate effective biocontrol strategies, samples were collected from 10 significantly infected areas at Southwest Forestry University; four to six infected leaves were gathered from each area, followed by the isolation and purification of strains from the infected plant leaves using tissue isolation and hyphae-purification techniques. We conducted an examination of the biological characteristics and compared the inhibitory effects of different concentrations of Phomopsis sp. (50%, 25%, 16.7%, 12.5%, and 10%) with 20 µg/mL of synthetic fungicides (Mancozeb, Carbendazim, Polyoxin, and Hymexazol) on the pathogen, while also assessing the control efficacy of Phomopsis sp. against the pathogen in the greenhouse. The internal transcribed spacer (ITS) region, β-tubulin (TUB), and translation elongation factor 1-alpha (TEF) analysis revealed that the highly virulent strain causing H. nepalensis leaf blight was Apiospora intestini. Additionally, it was found that 25% Phomopsis sp. significantly inhibited Apiospora intestini when compared to synthetic fungicides, and Phomopsis sp. supernatant possesses both protective and curative effects against the plant diseases caused by Apiospora intestini. The results of this study serve as a reference for the prevention and treatment of H. nepalensis leaf blight.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 3","pages":"103"},"PeriodicalIF":2.3,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143045968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabarcoding for the Monitoring of the Microbiome and Parasitome of Medically Important Mosquito Species in Two Urban and Semi-urban Areas of South Korea.","authors":"Xavier Chavarria, Jun Ho Choi, Singeun Oh, Myungjun Kim, Dongjun Kang, In-Yong Lee, Yun Soo Jang, Myung-Hee Yi, Tai-Soon Yong, Ju Yeong Kim","doi":"10.1007/s00284-025-04081-9","DOIUrl":"10.1007/s00284-025-04081-9","url":null,"abstract":"<p><p>Interactions between microbial communities and the host can modulate mosquito biology, including vector competence. Therefore, future vector biocontrol measures will utilize these interactions and require extensive monitoring of the mosquito microbiome. Metabarcoding strategies will be useful for conducting vector monitoring on a large scale. We used 16S and 18S rRNA gene metabarcoding through iSeq100 sequencing to characterize the microbiome and eukaryome of Aedes albopictus (Skuse 1894) and Culex pipiens (Linnaeus 1758), two globally important vectors present in South Korea. Mosquitoes were collected from an urban and a semi-urban location in South Korea. Bacterial alpha and beta diversities varied by population. Pseudomonadota dominated the microbiomes of both species. The microbiome composition varied by population and was dominated by different taxa. At the genus level, Wolbachia sp. was the most enriched genus in Cx. pipiens, followed by Aeromonas sp. In Ae. Albopictus, the most abundant group was Enterococcus sp. The gregarine parasite Ascogregarina taiwanensis was highly prevalent in Ae. Albopictus and its absence was marked by the presence of seven bacterial taxa. To our knowledge, this is the first characterization of the microbiome of Ae. albopictus and Cx. pipiens in these regions of South Korea and contributes to the current information on the microbiome of mosquito species, which can be used in further studies to assess pathogen-microbiome and microbiome-microbiome interactions.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 3","pages":"102"},"PeriodicalIF":2.3,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143045969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization, Identification, and Biosafety Evaluation of Multifaceted Traits of Plant Growth-Promoting Endophytes for Ameliorating Productivity of Forage Sorghum.","authors":"Jaspreet Kaur, Gulab Pandove, Amandeep Singh Sidhu, Sukhdeep Kaur Brar, Nitin Dev Singh, Vidhi Arora, Sohan Singh Walia","doi":"10.1007/s00284-025-04078-4","DOIUrl":"10.1007/s00284-025-04078-4","url":null,"abstract":"<p><p>Endophytes are bacteria that inhabit host plants for most of their life cycle without causing harm. In the study, 15 endophytic bacteria were isolated from 30 forage Sorghum plants and assessed for various plant growth-promoting (PGP) traits, such as phosphate solubilization, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity, ammonia production, siderophore production, gibberellic acid production, Indole-3-acetic acid (IAA) production, and zinc solubilization. One isolate, JJG_Zn, demonstrated multiple PGP activities and was identified as Enterobacter sp. through 16S rRNA sequencing. Considering the concept of 'One health,' a comprehensive biosafety analysis for any plant growth-promoting bacteria (PGP bacteria) is essential, thus, Enterobacter sp. (JJG_Zn), along with two other standard cultures, Burkholderia seminalis and Stenotrophomonas maltophilia, were evaluated for biosafety tests-including sheep blood agar assay, E.coli sensitivity assay, and pathogenicity tests on albino mice. The results indicated that all three PGP bacteria were potentially innocuous and compatible with each other. Furthermore, the field experiments were conducted at Punjab Agricultural University, Regional Research Station, Bathinda, and School of Organic Farming, IFS, Punjab Agricultural University, Ludhiana during Kharif 2022 employing a randomized complete block design (RCBD) with nine treatment combinations. The treatments included liquid bacterial inoculants (LBIs) of Stenotrophomonas maltophilia, Burkholderia seminalis, and Enterobacter sp. (JJG_Zn) applied in combination with 100% and 75% of the recommended dose of fertilizer (RDF), with each treatment replicated thrice. The treatment T₉ (75% RDF + dual inoculation with B. seminalis and S. maltophilia) resulted in the highest productivity, showing a 10.23% increase in pooled green fodder yield (GFY) compared to the control. The treatment T₈ (75% RDF + Enterobacter sp. (JJG_Zn)) showed a 5.14% increase in productivity over the control. Thus, liquid bacterial inoculants (LBIs) present a promising approach to enhance the productivity of forage Sorghum.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 3","pages":"101"},"PeriodicalIF":2.3,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143022660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reclassification of Salinisphaera halophila Zhang et al. 2012 as a Later Heterotypic Synonym of Salinisphaera orenii Park et al. 2012.","authors":"Syed Raziuddin Quadri, Manda Sathish, Ngoc Tung Quach, Wen-Jun Li, Manik Prabhu Narsing Rao","doi":"10.1007/s00284-024-04059-z","DOIUrl":"10.1007/s00284-024-04059-z","url":null,"abstract":"<p><p>In the present study, the taxonomic position of Salinisphaera halophila (NZ_AYKF00000000) and Salinisphaera orenii (NZ_AYKH00000000) was re-evaluated. In addition, their metabolic potentials and mechanisms for mitigating stress conditions were determined. Comparisons of 16S rRNA gene sequences, analysis of the phylogenetic tree, phylogenomic tree, average nucleotide identity (ANI), and digital DNA-DNA hybridization (dDDH) values were conducted. The 16S rRNA gene sequence similarity between Salinisphaera halophila YIM 95161^T and Salinisphaera orenii MK-B5^T was 100%. In phylogenetic and phylogenomic trees, Salinisphaera halophila YIM 95161^T and Salinisphaera orenii MK-B5^Tclustered together. Both species encode genes for glycolysis, citrate cycle, pentose phosphate pathway, Entner-Doudoroff pathway, nitrate assimilation, and assimilatory sulfate reduction. They employ salt-in and salt-out strategies to mitigate salt stress. The ANI and dDDH values between Salinisphaera halophila YIM 95161^Tand Salinisphaera orenii MK-B5^Twere 96.6 and 72.1%, respectively, above the cut-off (95-96% for ANI and 70% for dDDH) for species delineation. Based on the above results, we propose to reclassify Salinisphaera halophila Zhang et al. 2012 as a later heterotypic synonym of Salinisphaera orenii Park et al. 2012.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 3","pages":"100"},"PeriodicalIF":2.3,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly Potent New Probiotic Strains from Traditional Turkish Fermented Foods.","authors":"Mehmet Burak Yigit, Aysun Cebeci","doi":"10.1007/s00284-024-04045-5","DOIUrl":"10.1007/s00284-024-04045-5","url":null,"abstract":"<p><p>Traditional Turkish fermented foods like boza, pickles, and tarhana are recognized for their nutritional and health benefits, yet the probiotic potential of lactic acid bacteria (LAB) strains isolated from them remains underexplored. Sixty-six LAB strains were isolated from fermented foods using bacterial morphology, Gram staining, and catalase activity. The isolates were differentiated at strain level by RAPD-PCR (Random Amplification of Polymorphic DNA-Polymerase Chain Reaction) and twenty-five strains were selected for further evaluation of acid and bile salt tolerance. Among these, ten strains exhibited high tolerance and were subsequently assessed for adhesion to Caco-2 colorectal carcinoma cells, antimicrobial activity, exopolysaccharide (EPS) production, lysozyme resistance, and hemolytic activity. Using k-means clustering, three strains: Lactiplantibacillus plantarum ES-3, Pediococcus pentosaceus N-1, and Enterococcus faecium N-2 demonstrated superior probiotic characteristics, including significant acid (100% survival at pH3.0) and 0.3% bile salt tolerance (57%, 64%, 67%), strong adhesion to intestinal cells (65%, 88%, 91%), high lysozyme resistance (88%, 88%, 77%), and produced high amounts of EPS. These strains show promising potential as probiotics and warrant further investigation to confirm their functional properties and potential applications.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 2","pages":"97"},"PeriodicalIF":2.3,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}