Current Microbiology最新文献

{"title":"Natural Product-Based Strategies for Combating Antimicrobial Resistance in ESKAPE Pathogens.","authors":"Padum Lal, Anju Rana, Akash Dey, Alok Goyal, Sanjay M Jachak","doi":"10.1007/s00284-026-05166-9","DOIUrl":"10.1007/s00284-026-05166-9","url":null,"abstract":"<p><p>The increase of antimicrobial resistance (AMR), particularly among the ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter spp. poses a critical global threat to public health by severely limiting effective therapeutic options. These pathogens employ diverse and efficient survival strategies, including drug-degrading enzymes, target modification, porin loss, efflux pump overexpression, and biofilm formation, leading to multidrug-resistant (MDR), extensively drug-resistant (XDR), and pan-resistant clinical phenotypes. As conventional antibiotic discovery slows and treatment failures rise, natural products have re-emerged as a promising source of chemically diverse leads with antibacterial activity. Plants and microbes biosynthesize structurally diverse secondary metabolites such as polyketides, alkaloids, flavonoids, phenolics, terpenoids, and saponins, many of which exhibit broad-spectrum antibacterial activity and synergize with existing antibiotics while exerting reduced selective pressure for resistance. Several compounds demonstrated potent antibacterial activity with MIC values ranging from 0.1 to 32 µg/mL, highlighting their therapeutic potential. This review provides a comprehensive analysis of natural product-based strategies against ESKAPE pathogens, highlighting their mechanisms of action: membrane disruption, inhibition of efflux pumps, interference with DNA/protein synthesis, suppression of quorum sensing, and inhibition of biofilm formation. Special emphasis is placed on alkaloids, flavonoids, and terpenoids whose antibacterial potential is closely linked to defined structure-activity relationships (SAR). Alkaloids such as berberine, sanguinarine, and conessine show potent activity by targeting cell wall integrity, FtsZ-mediated division, and efflux systems, while specific flavonoid subclasses exhibited enhanced antibacterial potency through key structural features such as 5,7-dihydroxylation, prenylation, and optimized B-ring substitutions. Numerous natural products discussed demonstrate clinically relevant activity against MDR S. aureus, carbapenem-resistant K. pneumoniae, A. baumannii, and P. aeruginosa, supporting their potential as lead compounds or adjuvant agents.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 11","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149135291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Cross-Domain Framework for the Dual Nature of Geotrichum and Magnusiomyces Across Plant, Food, and Clinical Systems.","authors":"Sibel Derviş, Muharrem Türkkan, Göksel Özer","doi":"10.1007/s00284-026-05177-6","DOIUrl":"10.1007/s00284-026-05177-6","url":null,"abstract":"<p><p>The arthroconidial yeast genera Geotrichum and Magnusiomyces exhibit a striking dual nature, simultaneously acting as destructive postharvest pathogens, essential agents in dairy fermentation, and life-threatening opportunistic pathogens in immunocompromised hosts. Despite extensive domain-specific research, the mechanistic basis of this functional divergence remains poorly integrated across taxonomy, plant pathology, food microbiology, and clinical mycology. This review proposes a cross-domain framework that integrates taxonomic uncertainty, conserved molecular mechanisms, ecological plasticity, and exposure dynamics to explain this functional divergence. Here, \"cross-domain\" is used in a non-taxonomic sense and refers to integration across disciplinary and ecological/application contexts-plant pathology, food and dairy microbiology, and clinical mycology-rather than to the formal biological taxonomic rank Domain. The functional versatility of these yeasts can be partly interpreted through the context-dependent deployment of broadly conserved fungal mechanisms-including cell wall-degrading enzymes, CYP51-mediated sterol metabolism, secreted proteins/effectors, and stress-response pathways-operating under contrasting ecological and selective pressures, rather than by fundamentally distinct biological systems. Critically, this framework distinguishes unidirectional exposure pathways linking agricultural, food, and clinical environments from bidirectional feedback systems that may contribute to the emergence and circulation of adaptive traits. Within this perspective, we identify three cross-domain connections: a putative antifungal resistance cycle driven by converging agricultural and clinical azole selection, a dairy-clinical interface shaped by host-associated transmission and strain recombination, and a diagnostic blind spot linked to cross-reactivity in fungal detection systems. These insights are translated into testable hypotheses on genome-based species delimitation, resistance evolution, and domestication stability, providing a structured research agenda for coordinated risk assessment and resistance management within a One Health framework.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 11","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149126849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outbreak of Multidrug-Resistant (MDR) Acinetobacter baumannii ST-15 Strains Co-producing OXA-23 and OXA-58 in South America.","authors":"Aline Madeira Marques Saraiva, Amália Raiana Fonseca Lobato, Emanoele Saraiva Pereira, Maria Paula Cruz Schneider, Diego Assis das Graças, Adonney Allan de Oliveira Veras, Artur Silva, Danielle Murici Brasiliense, Rafael Azevedo Baraúna","doi":"10.1007/s00284-026-05160-1","DOIUrl":"10.1007/s00284-026-05160-1","url":null,"abstract":"<p><p>Acinetobacter baumannii is a high-priority pathogen due to its global dissemination and antimicrobial resistance. This work describes the first report of A. baumannii ST-15 isolates belonging to the International Clone 4 group, that exhibited an MDR profile with the co-production of OXA-23 and OXA-58 enzymes. Strains were isolated from an intensive care unit in a hospital of the Brazilian Amazon. Eight strains were recovered from an intensive care unit, showing high clonal stability across diverse clinical sources, including tracheal aspirates, urine, and blood, which underscores the high versatility and systemic dissemination potential of this lineage.The bla_OXA-23 and bla_OXA-58 genes were detected in all strains. A. baumannii isolates AB_OXA58_IEC07 and AB_OXA58_IEC02 were selected for hybrid genome sequencing and plasmid reconstruction, respectively, as representative samples of the outbreak. The reconstructed mobilome revealed a genome size of 4,110,050 bp, a GC content of 39.2%, and 3846 coding sequences for the reference strain, while four distinct plasmids were fully resolved.The bla_OXA-58 gene was detected flanked by ISAba3 and IS6-like elements.pdif modules were identified in another plasmid, together with mobilization and replication genes. The tet39 gene was also identified in a novel plasmid that is likewise mobilizable. VFDB found several genes related to biofilm formation and virulence. The identification of these high-risk genetic determinants within a monoclonal outbreak, involving diverse clinical sites, emphasizes the successful persistence of the ST-15 lineage in the hospital environment. This study describes a rare co-production of oxacillinases, highlighting the importance of constant genomic surveillance in monitoring the emergence of antibiotic-resistant pathogens that could pose a threat on a regional or global scale.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 11","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13642431/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149105857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Carbon Source on the Antifungal Activity of Lipopeptides Produced by Bacillus licheniformis SCV1 Against Trichophyton rubrum and Trichophyton mentagrophytes.","authors":"Chandana Malakar, Anushree Roy, Probodh Borah, Mojibur Rohman Khan, Ashis Kumar Mukherjee","doi":"10.1007/s00284-026-05176-7","DOIUrl":"10.1007/s00284-026-05176-7","url":null,"abstract":"<p><p>Biosurfactants are amphiphilic metabolites with antimicrobial properties, with their synthesis contingent upon the media utilized. This study investigated the antifungal activity of the biosurfactant generated by Bacillus licheniformis SCV1 grown in a mineral salt medium with various carbon sources. The research demonstrated that biosurfactants derived from media with several carbon sources, including glucose, glycerol, sucrose, starch, and mannitol, exhibited different antifungal efficacy against fungal pathogens, namely Trichophyton mentagrophytes and Trichophyton rubrum. The biosurfactant derived from the production medium containing glycerol as a carbon source showed superior antifungal efficacy against both fungi compared to biosurfactant produced from media with other carbon sources. FTIR and LC-MS analyses of the biosurfactants indicated that they are lipopeptides comprising of surfactin, iturin, and lichenysin subtypes. The lipopeptides produced using from various carbon sources exhibited structural variations in their fatty acyl chains. The biosurfactant derived from glycerol containing production medium was observed to include lipopeptides with extended fatty acyl chains, potentially contributing to its superior antifungal efficacy. Hence, this study emphasizes the significance of selecting an appropriate medium for generating biosurfactants with optimal activity against infections.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 11","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149105864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Post-infection Supplementation with Lactiplantibacillus plantarum CRD7 and Lacticaseibacillus rhamnosus CRD11 on Helicobacter pylori Virulence Gene Expression in a Rat Model of Gastric Infection.","authors":"Nazanin Sadat Seddighi, Ahad Ali Harati Asl, Mohammad Reza Asadi Karam, Seyed Reza Khatibi, Omid Azizi","doi":"10.1007/s00284-026-05112-9","DOIUrl":"10.1007/s00284-026-05112-9","url":null,"abstract":"<p><p>Helicobacter pylori infects nearly half of the global population and causes gastritis through virulence factors such as CagA and VacA. Increasing antibiotic resistance has reduced the effectiveness of conventional eradication therapies, highlighting the need for alternative strategies, including probiotics. This study evaluated the effects of post-infection supplementation with Lactiplantibacillus plantarum, Lacticaseibacillus rhamnosus, or their combination on cagA and vacA mRNA expression in a rat model of H. pylori infection. Male Wistar rats (n = 40) were randomly assigned to five groups (n = 8 each), including uninfected controls, H. pylori-infected untreated rats, and infected groups receiving L. plantarum CRD7, L. rhamnosus CRD11, or their combination. Infection was established by oral gavage and confirmed by rapid urease testing, bacterial isolation, and quantitative colony counting. Probiotics were administered orally after infection, and gastric tissues were collected for RNA extraction. Expression levels of cagA and vacA were quantified by qRT-PCR using the 2^(-ΔΔCt) method. H. pylori infection significantly increased cagA (2.7 ± 0.6-fold) and vacA (7.2 ± 0.7-fold) expression compared with controls. Probiotic supplementation reduced cagA expression to 1.86 ± 0.43-fold with L. rhamnosus, 1.2 ± 0.45-fold with L. plantarum, and 0.7 ± 0.8-fold with the combined treatment. Similarly, vacA expression decreased to 3.6 ± 0.44-fold, 1.2 ± 0.6-fold, and 1.7 ± 0.8-fold, respectively. One-way ANOVA showed a trend toward reduced cagA expression (p = 0.08) and significant differences in vacA expression among groups (p < 0.001). Post-infection supplementation with L. plantarum CRD7 and L. rhamnosus CRD11, particularly in combination, attenuated the expression of key H. pylori virulence-associated genes and may represent potential adjunctive strategies for modulating H. pylori virulence-associated gene expression.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 11","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149105845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Profiling of Bioactive Secondary Metabolites of Colletotrichum siamense Isolated from Catharanthus roseus with Emphasis on Moschamine by Advanced Chromatographic and Mass Spectrometric Techniques.","authors":"Jaya Prabha Khalkho, Ramesh Chandra","doi":"10.1007/s00284-026-05141-4","DOIUrl":"10.1007/s00284-026-05141-4","url":null,"abstract":"<p><p>Endophytic fungi are microorganisms that live in symbiotic relationships with plants, residing within their tissues without causing damage or disease to the host. Endophytic fungi provide vitality to the host plant and are excellent producers of many secondary metabolites that may contain pharmaceutical properties. In this study, Catharanthus roseus (L.) G. was collected from various places in Jharkhand to identify and isolate the endophytic fungi found in the leaf tissues of these plants. A total of 30 unique fungal isolates were obtained and maintained through subculturing at regular intervals. Among them, Colletotrichum siamense was chosen for targeted indole alkaloid compounds and their chemical profiling due to its bioactive potential. Primary screening of the metabolites was done through alkaloid tests and HPTLC analysis. A comprehensive metabolite profiling of the selected isolate has been performed by employing advanced analytical techniques such as high-performance liquid chromatography, gas chromatography-tandem mass spectrometry, and liquid chromatography-high-resolution mass spectrometry. The data obtained through GC-MS showed many bioactive constituents present within the fungal extracts and included: chlorozotocin, indole, 2-Amino-3-(4-hydroxyphenyl)-propanoic acid, 7-Methyl-Z-tetradecen-1-ol acetate, digitoxin, and glucobrassicin. In addition, LC-HRMS confirmed the presence of indole alkaloids found in the extracts of C. siamense, including moschamine and many other secondary metabolites. HPLC-DAD further analysed the fungal extracts, with quantification of moschamine at 109.13 µg/mL. The current research identified endophytic fungi isolated from C. roseus that may serve as valuable sources of pharmacologically active compounds. The observation of unique metabolic patterns among the isolates indicates that a variety of biosynthetic pathways exist within the endophytic fungi, as exemplified by the discovery of moschamine from Colletotrichum siamense.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 11","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149105275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic Antibacterial Mechanism of Ginger and Rosemary Essential Oils Against Shewanella putrefaciens.","authors":"Peibo Zheng, Zhe Shao, Xiaoyi Wei, Shucheng Liu, Xiaohong H Sun, Weiqing Q Lan","doi":"10.1007/s00284-026-05175-8","DOIUrl":"10.1007/s00284-026-05175-8","url":null,"abstract":"<p><p>This study investigated the synergistic antibacterial mechanism of ginger essential oil (GEO) and rosemary essential oil (REO) against Shewanella putrefaciens. Antibacterial efficacy and interactions were assessed by determining the minimum inhibitory concentration (MIC), fractional inhibitory concentration (FIC) index, and growth curves. Membrane integrity was evaluated using confocal laser scanning microscopy (CLSM), leakage of intracellular components, and alkaline phosphatase (AKPase) activity. Structural damage was observed via scanning electron microscopy (SEM). The effects on antioxidant defense and energy metabolism were analyzed by measuring superoxide dismutase (SOD), succinate dehydrogenase (SDH), and malate dehydrogenase (MDH) activities. Biofilm inhibition capacity was also examined. Results demonstrated a synergistic effect (FIC index = 0.375). The combination of GEO and REO caused severe membrane damage, intracellular leakage, inhibition of key enzyme activities, and effective biofilm inhibition. The synergistic mechanism involves membrane disruption, suppression of metabolic pathways, and biofilm inhibition.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 11","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149105230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploratory Associations Between Height-for-Age and Gut Microbiota Composition in Healthy School-Aged Children.","authors":"Elena Gomez-Gomez, Jorge García-Hernández, Ana Heredia, Joaquim Calvo-Lerma, Ana Andrés","doi":"10.1007/s00284-026-05173-w","DOIUrl":"10.1007/s00284-026-05173-w","url":null,"abstract":"<p><p>Body mass index (BMI) is frequently used to investigate associations between gut microbiota and nutritional status in children, although it primarily reflects adiposity and may not fully capture broader aspects of physiological development. Height-for-age z-score (HAZ), an indicator of linear growth, may provide complementary information on microbiota-host interactions during childhood. This exploratory cross-sectional study investigated associations between anthropometric indicators and gut microbiota composition in a cohort of healthy Spanish school-aged children. Forty-five children aged 8-12 years were included. Faecal microbiota composition was analysed by 16S rRNA gene sequencing, and associations with anthropometric variables were evaluated using correlation and multivariable regression analyses. The gut microbiota was dominated by Bacillota and Bacteroidota, with limited variability in overall community structure across the cohort. BMI-for-age was not associated with microbial diversity, taxonomic composition or the Bacillota/Bacteroidota ratio. In contrast, HAZ was negatively associated with the Bacillota/Bacteroidota ratio, and this association remained significant after adjustment for BMI-for-age. No significant differences in alpha or beta diversity were detected according to anthropometric indicators. These findings suggest a possible relationship between linear growth and gut microbiota composition in healthy children. Given the limited sample size and cross-sectional design, the results should be considered exploratory and hypothesis-generating. Further studies are needed to confirm these observations and clarify the potential role of gut microbial ecology in childhood growth.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 11","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149105876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Core Proteome Subtractive Genomics Reveals Potential Therapeutic Targets in Bacillus anthracis.","authors":"Raison Joseph, Subalakshmi Kaarmegam Pandiarajan, Jeyaprakash Rajendhran","doi":"10.1007/s00284-026-05168-7","DOIUrl":"10.1007/s00284-026-05168-7","url":null,"abstract":"<p><p>Bacillus anthracis, a Gram-positive, spore-forming bacterium, poses significant public health risks due to its zoonotic potential and historical use in bioterrorism. Pathogenicity of B. anthracis is primarily attributed to its genetic components, including the essential plasmids pXO1 and pXO2. The pXO1 plasmid encodes toxic factors, while pXO2 facilitates immune evasion. The zoonotic potential, as well as potential bio-threat, necessitates investigation into novel therapeutic targets. Through metabolic network analysis, docking, and notably subtractive genomics, researchers have effectively pinpointed pathogen-specific target proteins essential for drug development. Subtractive genomics, in particular, stands out for its cost-effectiveness and ability to identify non-host homologous targets. In this study, we used a core proteome immunoinformatic approach to identify potential therapeutic targets in B. anthracis. A total of 3987 proteins were found to be conserved in B. anthracis and among them, 388 were essential, non-human homologous proteins. These proteins were then subjected to localization and pathway analysis which resulted in 79 unique pathway proteins. Through PPI and druggability analysis, we had identified 17 strain-independent therapeutic target proteins. All the identified proteins are non-homologous to human proteins and, therefore, are potential targets for drug development. These potential target proteins are involved primarily in cell division, peptidoglycan biosynthesis, and putative flagellar assembly, which are essential for bacterial survival and pathogenicity. These identified target proteins could be the foundation for the development of therapeutics against human anthrax.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 11","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149105878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preventive Activity of Paroxetine Against the Formation of Mixed Biofilms of Candida albicans and Staphylococcus aureus on Peripheral Venous Catheters.","authors":"Lívia Gurgel do Amaral Valente Sá, Érica Rayanne Mota da Costa, Maria Janielly Castelo Branco Silveira, Vitória Pessoa de Farias Cabral, Henrique Santos Carneiro, Leilson Carvalho de Oliveira, Thais Lima Ferreira, Daniel Sampaio Rodrigues, Lara Elloyse Almeida Moreira, Vitória Monteiro Matos, Vitória Girão Dos Santos, João Batista de Andrade Neto, Hélio Vitoriano Nobre Júnior, Cecília Rocha da Silva","doi":"10.1007/s00284-026-05088-6","DOIUrl":"https://doi.org/10.1007/s00284-026-05088-6","url":null,"abstract":"<p><p>Catheters are often sources of bloodstream infections since they promote the development of polymicrobial biofilms composed of Candida albicans and Staphylococcus aureus, which are resistant to conventional treatments. Paroxetine (PRX), a selective serotonin reuptake inhibitor, has demonstrated antibiofilm activity against both C. albicans and S. aureus. However, studies evaluating PRX's activity against polymicrobial biofilms remain scarce. Consequently, we investigated the activity of PRX against mixed biofilms of C. albicans and S. aureus, as well as its potential to prevent the formation of such biofilms on peripheral venous catheters. Our findings revealed that PRX not only prevented biofilm formation but also significantly reduced both the viability and biomass of these structures, possibly by disrupting the polysaccharide matrix. When impregnated in peripheral venous catheters, PRX also inhibited the excessive growth of microbial communities, making it a potentially effective agent to modulate the structure and susceptibility of multispecies biofilms, expanding its spectrum of action to more complex clinical scenarios associated with medical devices.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 11","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13630820/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149105272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}