Current Microbiology最新文献_第5页

Deletion of MSMEG_3118, an ABC Transporter Family Protein in Mycobacterium smegmatis Alters Lipids Profile and Drug Susceptibility. 耻垢分枝杆菌ABC转运蛋白家族蛋白MSMEG_3118的缺失改变了脂质谱和药物敏感性

IF 2.8 3区生物学

Current Microbiology Pub Date : 2026-10-01 DOI: 10.1007/s00284-026-05143-2

Gunjan, Rajendra Kumar Dhuriya, Garima Kumari, Sandeep Sharma, Mohd Mustkim Ansari, Bhupendra N Singh

{"title":"Deletion of MSMEG_3118, an ABC Transporter Family Protein in Mycobacterium smegmatis Alters Lipids Profile and Drug Susceptibility.","authors":"Gunjan, Rajendra Kumar Dhuriya, Garima Kumari, Sandeep Sharma, Mohd Mustkim Ansari, Bhupendra N Singh","doi":"10.1007/s00284-026-05143-2","DOIUrl":"10.1007/s00284-026-05143-2","url":null,"abstract":"Multidrug resistant tuberculosis (MDR-TB) poses a serious global health concern. The development of drug-resistant mycobacterial strains is likely influenced by efflux transporters. In this study we created a deletion mutant of MSMEG_3118, a hypothetical efflux pump protein in Mycobacterium smegmatis, and characterized the mutant. It is shown that MSMEG_3118 acts as an efflux pump, and its deletion results in enhanced accumulation of the ethidium bromide. The MSMEG_3118 deletion mutant exhibited noticeable changes in colony morphology, slower growth rates, and reduced biofilm formation, indicating a larger role of this protein in mycobacterial physiology. Lipids profile showed elevated levels of menaquinone in the mutant. Interestingly, the mutant showed increased sensitivity to clofazimine and the proton motive force inhibitor CCCP but rendered higher resistance to several first- and second-line anti-tubercular drugs, such as rifampicin, isoniazid, streptomycin, vancomycin and erythromycin. All the changes in the mutant phenotypes were duly restored in the complemented mutant strain, suggesting a larger role of this protein in addition to an efflux pump transporter. Summarily, these results highlight a complex regulatory network that contributes to the multidrug resistance in mycobacteria.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 11","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149105909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Role of Porin Proteins and ygiU-nhaR in the Formation of Biofilm in Escherichia coli. 孔蛋白和ygiU-nhaR在大肠杆菌生物膜形成中的作用。

IF 2.8 3区生物学

Current Microbiology Pub Date : 2026-09-29 DOI: 10.1007/s00284-026-05171-y

Fatma Er, Özge Kaygusuz İzgördü, Gülçin Çetin Kılıçaslan, Cihan Darcan

{"title":"The Role of Porin Proteins and ygiU-nhaR in the Formation of Biofilm in Escherichia coli.","authors":"Fatma Er, Özge Kaygusuz İzgördü, Gülçin Çetin Kılıçaslan, Cihan Darcan","doi":"10.1007/s00284-026-05171-y","DOIUrl":"10.1007/s00284-026-05171-y","url":null,"abstract":"This study aimed to investigate the roles of porin proteins (OmpA, OmpC, OmpF, OmpG, OmpT, LamB, and PhoE) in biofilm formation in Escherichia coli. The effect of environmental factors such as pH (5.5, 6.0, 6.5, 7.0, 7.5, and 8.0) and biologically essential metal ions (copper, nickel, and zinc) on biofilm formation was also revealed. The ompA, ompC, and lamB porin mutants formed biofilms at different pH values, whereas no biofilm formation was detected in the wild type and ompF, ompG, ompT, and phoE mutants. While the ompC mutant formed a biofilm across a wide pH range (5.5-8.0), the ompA mutant formed a medium-level biofilm at pH values between 6.5 and 8.0. It was observed that the lamB mutant strain produced biofilm only at pH 7.0 and 7.5. Biofilm formation in the ompA and lamB mutants showed little change following metal supplementation, whereas increased biofilm formation was observed in the ompC mutant in the presence of copper and nickel. The biofilm formed in mutants was inhibited in the presence of zinc. Altered expression of nhaR, particularly ygiU, was found to be associated with biofilm formation. In the ompA, ompC, and lamB porin-deficient strains, increased ygiU expression and, to a lesser extent, altered nhaR expression were observed. Considering all these results, it can be concluded that E. coli W3110, which does not produce biofilms under normal physiological conditions, may acquire a biofilm-forming phenotype following mutations in porin genes.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 11","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149079820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of Ansamitocin P-3 Biosynthesis by the Response Regulator CrsB in Actinosynnema pretiosum. 反应调节因子CrsB对放线菌综合征中抗菌肽P-3生物合成的调控。

IF 2.8 3区生物学

Current Microbiology Pub Date : 2026-09-29 DOI: 10.1007/s00284-026-05169-6

Tengfei Wang, Lingjia Zhu, Ruoyi Sun, Shuangying Zhao, Siyu Wang, Ruixin Huang, Meng Liu, Guangxiang Cao, Peipei Zhang

{"title":"Regulation of Ansamitocin P-3 Biosynthesis by the Response Regulator CrsB in Actinosynnema pretiosum.","authors":"Tengfei Wang, Lingjia Zhu, Ruoyi Sun, Shuangying Zhao, Siyu Wang, Ruixin Huang, Meng Liu, Guangxiang Cao, Peipei Zhang","doi":"10.1007/s00284-026-05169-6","DOIUrl":"10.1007/s00284-026-05169-6","url":null,"abstract":"Ansamitocin P-3 (AP-3) is an ansamycin antibiotic produced by Actinosynnema pretiosum with high clinical and commercial value. Its biosynthesis is tightly controlled, yet the underlying transcriptional network is not fully understood. Here, we identified the two-component system CrsAB and characterized the response regulator CrsB. Deletion of crsB resulted in a 57% reduction in AP-3 yield without impairing morphological development or biomass accumulation. RNA‑Seq showed that CrsB acts as a global regulator, influencing energy metabolism, cell integrity, and biosynthetic capacity. At the fermention at 48 h, genes asm23 and asm43-47 within the biosynthetic gene cluster, which are primarily responsible for the synthesis of AHBH from UDP-glucose, were significantly downregulated upon crsB deletion. Conversely, genes of the pentose phosphate pathway, zwf, opcA and pgl, were markedly upregulated, whereas their expression levels showed no significant differences between the two strains at 72 h. These temporal transcriptional changes were confirmed by qRT‑PCR. Collectively, our data suggest that CrsB positively coordinates primary and secondary metabolism primarily during the early stage to promote AP-3 production, offering new targets for metabolic engineering.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 11","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149079800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beyond the Rumen: Current Evidence and Knowledge Gaps in Microbial Diversity and Function Across the Bovine Gastrointestinal Tract. 超越瘤胃：牛胃肠道微生物多样性和功能的现有证据和知识差距。

IF 2.8 3区生物学

Current Microbiology Pub Date : 2026-09-29 DOI: 10.1007/s00284-026-05159-8

Abiodun Mayowa Akanmu

{"title":"Beyond the Rumen: Current Evidence and Knowledge Gaps in Microbial Diversity and Function Across the Bovine Gastrointestinal Tract.","authors":"Abiodun Mayowa Akanmu","doi":"10.1007/s00284-026-05159-8","DOIUrl":"10.1007/s00284-026-05159-8","url":null,"abstract":"The bovine gastrointestinal tract (GIT) is a spatially organised microbial ecosystem, but the evidence supporting a whole-tract interpretation is highly uneven. This critical review distinguishes well-established rumen biology from emerging observations in the reticulum, omasum, abomasum, small intestine and hindgut. Comparative studies show strong regional filtering of microbial communities, yet most available datasets are cross-sectional, use digesta rather than mucosa, and infer function from DNA. Consequently, the presence of genes or taxa cannot be equated with active metabolism. Early life provides an important developmental dimension: microbial succession during the milk-to-solid-feed transition accompanies rumen maturation and region-specific immune development, although durable effects on adult productivity remain incompletely demonstrated. Across adult cattle, foregut fermentation supplies most microbially derived energy, whereas downstream compartments support residual fermentation, epithelial interactions and barrier-related processes whose quantitative contributions are less certain. Methanogenesis is therefore treated as one outcome among nutrition, immune function, pathogen resistance and gut integrity. Metagenomics, metatranscriptomics, metaproteomics and metabolomics are complementary rather than interchangeable; coordinated sampling is required to connect functional potential to activity and host phenotype. Priority should be given to longitudinal, multi-compartment, mucosa-and-digesta studies with absolute microbial measurements, metabolite fluxes and transparent causal inference. A tract-wide framework is valuable not because all compartments are equally understood, but because it makes the present evidence imbalance explicit and identifies where microbiome-targeted nutrition can be tested responsibly.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 11","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13624028/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149079738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Building a Global Innovation Pipeline for Organometallic Antifungals Against Nakaseomyces glabratus: Correspondence on Maqueda-Cabrera and Cuéllar-Cruz. 有机金属抗秃中霉全球创新管道的构建：Maqueda-Cabrera和cu<s:1> - cruz的通信。

IF 2.8 3区生物学

Current Microbiology Pub Date : 2026-09-29 DOI: 10.1007/s00284-026-05165-w

Deen Dayal, Kundan Kumar Chaubey, Rajesh Prasad Jayaswal, Syed Mohd Danish Rizvi, Ashish Vyas

{"title":"Building a Global Innovation Pipeline for Organometallic Antifungals Against Nakaseomyces glabratus: Correspondence on Maqueda-Cabrera and Cuéllar-Cruz.","authors":"Deen Dayal, Kundan Kumar Chaubey, Rajesh Prasad Jayaswal, Syed Mohd Danish Rizvi, Ashish Vyas","doi":"10.1007/s00284-026-05165-w","DOIUrl":"10.1007/s00284-026-05165-w","url":null,"abstract":"Maqueda-Cabrera and Cuéllar-Cruz reported dose-dependent activity of the organometallic compound N-(2-hydroxyethyl)ferroceneselenoamide (FE1) against Nakaseomyces glabratus in a Galleria mellonella infection model. FE1 at 20 mg/kg produced complete larval protection and reduced fungal burden relative to fluconazole tested at the same dose. These findings are promising but remain preliminary because only the reference strain CBS138 was evaluated and the compound's mechanism of action is unknown. Further work should test geographically and genetically diverse clinical isolates, azole- and echinocandin-resistant phenotypes, and other clinically important opportunistic yeasts. Mechanistic studies should examine fungal stress-response pathways, resistance evolution, toxicity, pharmacokinetics/pharmacodynamics, and larval immune responses. G. mellonella is best positioned as a pre-screening or intermediate-screening experimental model rather than a direct predictor of clinical efficacy. A staged programme that links reproducible invertebrate endpoints to mammalian validation and WHO fungal-priority objectives could clarify whether FE1 merits further development as an antifungal candidate.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 11","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149079752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whole Genome Sequencing Reveals Antibiotic Resistance Heterogeneity and Metabolic Signatures of Cutibacterium acnes in Atopic Dermatitis. 全基因组测序揭示了特应性皮炎中痤疮表皮杆菌的抗生素耐药性异质性和代谢特征。

IF 2.8 3区生物学

Current Microbiology Pub Date : 2026-09-26 DOI: 10.1007/s00284-026-05167-8

Tianze Yu, Yuwen Gao, Xiaoqiang Xu, Yang Liu, Xu Yao, Shi Wu, Wei Li

{"title":"Whole Genome Sequencing Reveals Antibiotic Resistance Heterogeneity and Metabolic Signatures of Cutibacterium acnes in Atopic Dermatitis.","authors":"Tianze Yu, Yuwen Gao, Xiaoqiang Xu, Yang Liu, Xu Yao, Shi Wu, Wei Li","doi":"10.1007/s00284-026-05167-8","DOIUrl":"10.1007/s00284-026-05167-8","url":null,"abstract":"Cutibacterium acnes (C. acnes), the most abundant microbe of the skin microbiota, has been reported playing a protective role in atopic dermatitis (AD), in contrast to its proinflammatory role in acne. While antibiotic resistance in C. acnes has been extensively studied in acne, little is known about its characteristics in AD. Here, we performed an integrated genomic and functional analysis of 405 AD-derived C. acnes isolates using whole genome sequencing (WGS) and antimicrobial susceptibility testing. The result showed that antibiotic resistance genes (ARGs) were highly clustered within specific individuals and phylogenetic subtypes of C. acnes, with erm(X) being the most prevalent determinant conferring cross-resistance to erythromycin and clindamycin. To verify the high genotype-phenotype concordance (95.45%) of erythromycin and clindamycin resistance of 110 ARGs-carried C. acnes isolates, minimum inhibitory concentration (MIC) was performed. AD isolates exhibited low-level acquired resistance to tetracyclines, chloramphenicol, and fusidic acid, indicating distinct antibiotic exposure pressures in AD patients. Comparative genomic analysis and genome-wide association study both showed that metabolism and quorum sensing related pathways were enriched in pan-genome of ARG-containing strains. These findings suggest that resistance in AD-associated C. acnes is linked to metabolic adaptations that may facilitate survival in the AD skin environment. Together, these findings provide a genomic and functional framework for understanding the antibiotic resistance of AD-associated C. acnes, underscoring the need for careful antibiotic stewardship in AD management.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 11","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149059338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physicochemical and Bioactive Characterization of a Neotropical Calothrix Strain. 一株新热带卡思菌的理化及生物活性研究。

IF 2.8 3区生物学

Current Microbiology Pub Date : 2026-09-26 DOI: 10.1007/s00284-026-05113-8

Camila Andrea Marín-Marín, Luis C Beltrán, Paola Andrea Zapata Ocampo

{"title":"Physicochemical and Bioactive Characterization of a Neotropical Calothrix Strain.","authors":"Camila Andrea Marín-Marín, Luis C Beltrán, Paola Andrea Zapata Ocampo","doi":"10.1007/s00284-026-05113-8","DOIUrl":"10.1007/s00284-026-05113-8","url":null,"abstract":"Despite the biotechnological potential of cyanobacteria, the antibacterial, antifungal, and prebiotic properties of the genus Calothrix remain largely unexplored. This study characterizes the composition and biological activity of a neotropical strain Calothrix sp. CBUCES-K 9914 from Antioquia, Colombia, and evaluates growth under varying light and nutrient treatments. The strain achieved its highest biomass yield (0.75 g L⁻¹) under white light supplemented with 0.3 g L⁻¹ glucose and 0.2 g L⁻¹ nitrogen. Characterization revealed 16 amino acids, dominated by aspartic acid (20.4%), alanine (14.5%), and glycine (13.9%), alongside high concentrations of sodium (3.30 mg g⁻¹) and calcium (2.90 mg g⁻¹). Antioxidant assays showed significant activity, particularly in the ORAC assay (118.2 ± 12.9 µmol TE g⁻¹). The strain significantly inhibited the growth of three pathogenic bacteria: Klebsiella pneumoniae ATCC 4352, Salmonella enterica ATCC 14028, and Staphylococcus aureus ATCC 6538; Kruskal-Wallis Tests: χ2 = 18.33-40.89, p < 0.01) and significantly suppressed growth in one yeast, Candida albicans ATCC 10231, beginning at hour 16 (χ2 = 17.8, p = 0.003) and persisting through hour 41 (χ2 = 16.48, p = 0.005). Conversely, it promoted the growth of two probiotic bacteria, Lactiplantibacillus plantarum and Limosilactobacillus reuteri, from hour 17 to 27.5 (χ2 = 11.48-21.33, p < 0.05). These findings highlight the biotechnological potential of strain Calothrix sp. CBUCES-K 9914 and warrant deeper investigation into the metabolite profile and the mechanisms underlying its antimicrobial and prebiotic activities.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 11","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149059343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Characterization of a Novel Mycovirus Isolated from Binucleate Rhizoctonia AG‑Bb strain 8-Q-36. 双核根丝核菌AG‑Bb株8-Q-36分离的一种新型分枝病毒的分子特性

IF 2.8 3区生物学

Current Microbiology Pub Date : 2026-09-24 DOI: 10.1007/s00284-026-05164-x

Runshuang Qiu, Ningwei Chen, Lihua Zhao, Yan Zhang, Chengyun Li, Genhua Yang

引用次数: 0

Semi-continuous Cultivation of Scenedesmus quadricauda for High Biomass Production Utilizing the Byproduct Huangshui from Chinese Liquor Brewing. 利用白酒酿造副产黄水半连续栽培高产生物质四角田葵。

IF 2.8 3区生物学

Current Microbiology Pub Date : 2026-09-22 DOI: 10.1007/s00284-026-05156-x

Jiajing Yan, Yuting Luo, Yuliang Zhang, Chuan Wang

{"title":"Semi-continuous Cultivation of Scenedesmus quadricauda for High Biomass Production Utilizing the Byproduct Huangshui from Chinese Liquor Brewing.","authors":"Jiajing Yan, Yuting Luo, Yuliang Zhang, Chuan Wang","doi":"10.1007/s00284-026-05156-x","DOIUrl":"10.1007/s00284-026-05156-x","url":null,"abstract":"Huangshui, a by-product of Chinese Liquor brewing, is rich in diverse organic compounds. In this study, the growth and biomass production of Scenedesmus quadricauda were investigated using Huangshui as culture substrate. The optimal cultivation conditions were identified as follows: pH 7.0, Huangshui concentration of 5%, cultivation duration of 6 days, and light intensity of 8000 lx. Under semi-continuous cultivation with a renewal rate of 30%, an initial cell density of 2.5 × 105 cells/mL and a renewal period of 2 days, S. quadricauda achieved a biomass concentration of 3.70 g/L and a productivity 550 mg/L-1d- 1. These values represented increases of 55.5% and 109.9%, respectively, compared to those obtained in batch culture. Notably, the protein content and protein productivity of S. quadricauda reached 49.152% and 272.78 mg/L-1d- 1, respectively, with a 38.9% increase in essential amino acid content relative to batch culture. This study appears to be the first report to cultivate microalgae in Huangshui. The Huangshui, derived from fermentation, showed considerable potential for use as a high-performance microalgae culture medium.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 11","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149024063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physiological Mechanisms of the Endophytic Fungus Phomopsis liquidambaris B3 in Enhancing Drought Tolerance in Rice. 内生真菌液蕨B3增强水稻抗旱性的生理机制

IF 2.8 3区生物学

Current Microbiology Pub Date : 2026-09-22 DOI: 10.1007/s00284-026-05161-0

Yang Yang, Qing-Yun Gu, Xiao-Han Wu, Shu-Yuan Zhang, Ming-Hui Xu, Chao Dai, Chuan-Chao Dai

{"title":"Physiological Mechanisms of the Endophytic Fungus Phomopsis liquidambaris B3 in Enhancing Drought Tolerance in Rice.","authors":"Yang Yang, Qing-Yun Gu, Xiao-Han Wu, Shu-Yuan Zhang, Ming-Hui Xu, Chao Dai, Chuan-Chao Dai","doi":"10.1007/s00284-026-05161-0","DOIUrl":"10.1007/s00284-026-05161-0","url":null,"abstract":"With the popularization of upland rice cultivation and the intensification of global warming, drought tolerance in rice has become a critical agronomic challenge. Plant endophytes can establish symbiotic relationships with host plants and enhance plant stress tolerance. Previous studies have demonstrated that Phomopsis liquidambaris B3 (P. liquidambaris B3) can form a stable symbiosis with rice and improve plant disease resistance. However, whether this strain can enhance rice drought tolerance remains unclear. In this study, a 7-day water-withholding treatment was imposed on rice at the seedling, tillering, and booting stages. Physiological and biochemical indices related to drought tolerance were determined in plants inoculated with P. liquidambaris B3 and uninoculated control plants.The results showed that drought stress induced obvious leaf rolling in uninoculated rice, whereas no significant leaf rolling was observed in B3-inoculated plants. Under drought conditions, the relative water content, photosynthetic performance (chlorophyll content and net photosynthetic rate), osmotic adjustment substances (proline, soluble protein and soluble sugar), and antioxidant enzyme activities (superoxide dismutase, catalase and peroxidase) in B3-inoculated rice were significantly higher than those in the uninoculated control. Meanwhile, stomatal conductance and transpiration rate were markedly lower in the B3 inoculation group. In addition, plant growth performance and grain yield were significantly improved by B3 inoculation. P. liquidambaris B3 effectively promoted rice photosynthetic capacity under drought stress.Collectively, these findings indicate that P. liquidambaris B3 enhances rice drought tolerance by optimizing physiological performance, promoting plant growth, and ultimately increasing grain yield. This study provides a promising theoretical and application basis for improving drought tolerance in rice.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"83 11","pages":""},"PeriodicalIF":2.8,"publicationDate":"2026-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149023951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0