Current Microbiology最新文献_第6页

Repurposing Plant-Based Histone Acetyltransferase Inhibitors: A Review of Novel Therapeutic Strategies Against Drug-Resistant Fungal Biofilms. 植物组蛋白乙酰转移酶抑制剂的再利用：针对耐药性真菌生物膜的新型治疗策略综述》。

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-11-12 DOI: 10.1007/s00284-024-03971-8

Tamil Selvam Saravanan, Chaitany Jayprakash Raorane, Vinothkannan Ravichandran, Satish Kumar Rajasekharan

引用次数: 0

Investigating Microbial Diversity in the Endosphere and Rhizoplane of Three Aromatic Rice Landraces: Implications for Biological Nitrogen Fixation. 调查三个芳香水稻品种内圈和根瘤中的微生物多样性：生物固氮的意义。

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-11-11 DOI: 10.1007/s00284-024-03978-1

Papri Nag, Yenkokpam Supriya, Jhuma Datta, Soumen Bera, Sampa Das

{"title":"Investigating Microbial Diversity in the Endosphere and Rhizoplane of Three Aromatic Rice Landraces: Implications for Biological Nitrogen Fixation.","authors":"Papri Nag, Yenkokpam Supriya, Jhuma Datta, Soumen Bera, Sampa Das","doi":"10.1007/s00284-024-03978-1","DOIUrl":"10.1007/s00284-024-03978-1","url":null,"abstract":"Biological nitrogen fixation (BNF) occurring in the rhizosphere is a sustainable source of nitrogen for plants. BNF in cereal crops can be promoted by inoculation of a single or consortium of associative and endophytic diazotrophs. Creating a successful nitrogen-fixing biofertilizer necessitates the study of the core microbiome of the plant rhizosphere and the functional relationship of the members. This study compares the endosphere and rhizoplane microbial diversity of three aromatic landraces and one high-yielding variety of rice using culture-independent methods. The V3-V4 variable regions of 16S rRNA were used for amplicon sequencing of soil DNA. Patescibacteria, Planctomycetota, and Proteobacteria were the predominant phyla in all four genotypes. Richness (Chao-1 and ACE) and microbial diversity indices (Shannon and Simpson indices) showed that microbial diversity among the genotypes varies subtly at the phylum level. Beta diversity analysis with the phylum identified and comparisons of the microbiome at the genus level revealed a more prominent effect of plant genotype on microbial diversity. Canonical component analysis drew a correlation between microbial diversity in each genotype with the sugar and N content of these landraces. Paraburkholderia was identified as one of the major OTUs among the known nitrogen fixing followed by Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium group, Azospirillum, Hebarspirillum, and Azotobacter.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"81 12","pages":"454"},"PeriodicalIF":2.3,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142616776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nostoxanthin Biosynthesis by Sphingomonas Species (COS14-R2): Isolation, Identification, and Optimization of Culture Conditions. 鞘氨醇单胞菌（COS14-R2）的 Nostoxanthin 生物合成：分离、鉴定和优化培养条件。

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-11-11 DOI: 10.1007/s00284-024-03956-7

Jegadeesh Raman, Jeong-Seon Kim, Young-Joon Ko, Soo-Jin Kim

{"title":"Nostoxanthin Biosynthesis by Sphingomonas Species (COS14-R2): Isolation, Identification, and Optimization of Culture Conditions.","authors":"Jegadeesh Raman, Jeong-Seon Kim, Young-Joon Ko, Soo-Jin Kim","doi":"10.1007/s00284-024-03956-7","DOIUrl":"10.1007/s00284-024-03956-7","url":null,"abstract":"Nostoxanthin, a yellow pigment, belongs to the xanthophyll group of carotenoids found in various species of bacteria and cyanobacteria. Several species of Sphingomonas can produce appropriate carotenoids for survive in various environments. This comprise nostoxanthin, a significant carotenoid. The study isolated the Sphingomonas species strain COS14-R2 from the Cosmos bipinnatus and identified it through the whole-genome sequence. The strain consists of a circular chromosome with a length of 3,677,457 base pairs.s ecThe genome consists of three carotenoid biosynthesis genes, specifically crtB (phytoene synthase), crtI (phytoene desaturase), and crtY (Lycopene beta-cyclase), which are involved in the synthesis of nostoxanthin. The strain has a circular, undulated colony morphology with a deep yellow color. It demonstrates optimal growth in liquid media at 25 to 35 °C and exhibits a high tolerance for pH levels between 5 and 11 and requires adequate quantities of carbon and nitrogen. We observed the highest concentration of nostoxanthin was recorded at 35 °C, pH of 7.5, glucose concentration of 40 g L-1, and a yeast extract concentration of 5 g L-1 during dark incubation. The fed-batch fermentation process produced nostoxanthin at a concentration of 217.22 ± 9.60 mg L-1, with a selectivity of 72.32% and a productivity of 2.59 g/L/h. The dry biomass extract was purified using column chromatography. The LC-MS/MS analysis of the purified fraction indicated that the molecular weight of nostoxanthin is 600.5098 m/z. The DPPH assay result of 75.5 ± 0.33% indicates nostoxanthin is highly effective in scavenging free radicals.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"81 12","pages":"453"},"PeriodicalIF":2.3,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11554843/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142616798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening, Gene Cloning and Expression of Cellulase-Producing Strain Bacillus subtilis Xh-16. 纤维素酶生产菌株枯草芽孢杆菌 Xh-16 的筛选、基因克隆和表达。

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-11-11 DOI: 10.1007/s00284-024-03961-w

Xiaoxi Zeng, Xinping Ren, Ruotong Wu, Yuanke Zhang, Cheng Zhang, Song Ran, Liang Ma

{"title":"Screening, Gene Cloning and Expression of Cellulase-Producing Strain Bacillus subtilis Xh-16.","authors":"Xiaoxi Zeng, Xinping Ren, Ruotong Wu, Yuanke Zhang, Cheng Zhang, Song Ran, Liang Ma","doi":"10.1007/s00284-024-03961-w","DOIUrl":"10.1007/s00284-024-03961-w","url":null,"abstract":"Cellulase is a complex enzyme system composed of multiple hydrolytic enzymes. It can degrade cellulose into glucose and improve the utilization efficiency of cellulose resources. Cellulase produced by microorganisms is the main method used in industry, offering the advantages of convenience and being environmentally friendly. A strain Xh-16 with high cellulase production was screened from the rotten rice straw. It was identified as Bacillus subtilis by morphological identification, physiological and biochemical identification, and 16S rRNA gene sequence analysis. Strain Xh-16 was used to degrade rice straw. After a 48 h cellulase treatment, the complete degradation and structural breakdown of the straw were observed. We cloned the endoglucanase Cel5L gene of Bacillus subtilis Xh-16 and induced expression of the cloned gene in Escherichia coli BL21 (DE3). The results showed that the coding length of Cel5L gene was 1500 bp, and the molecular weight of the encoded protein was about 55 kDa. The molecular formula is C2456H3811N671O761S10 and it has 7709 atoms and 499 amino acids. Cel5L differs significantly from some the glycoside hydrolase family 5 cellulases because it has only one carbohydrate binding module family 3 at the C-terminus of its catalytic domain. The cellulase gene Cel5L in Xh-16 can encode active cellulase and can be heterologously expressed in Escherichia coli, which makes Escherichia coli have cellulase function. This study serves as a foundation for further research on cellulase diversity in Bacillus subtilis and offers insights for enhancing cellulase production.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"81 12","pages":"452"},"PeriodicalIF":2.3,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142616841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Nicotine on Pulmonary Pathogenic Bacteria. 尼古丁对肺部致病细菌的影响

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-11-08 DOI: 10.1007/s00284-024-03977-2

Adrián Rodríguez-Carlos, Oscar E Gonzalez-Muniz, Maria G Ramirez-Ledesma, Bruno Rivas-Santiago

引用次数: 0

Integrative Proteome and Metabolomics Analyses of Cryptococcus neoformans Responses to Melanin Substrates Niger seed and L-DOPA. 新型隐球菌对黑色素底物尼日尔种子和 L-DOPA 反应的蛋白质组和代谢组学综合分析。

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-11-08 DOI: 10.1007/s00284-024-03979-0

Yuanyuan Ma, Peng Xue

引用次数: 0

Assessing Process Conditions on Xylose Fermentation in Spathaspora passalidarum: Effects of pH, Substrate-to-Inoculum Ratio, Temperature, and Initial Ethanol Concentration. 评估 Spathaspora passalidarum 中木糖发酵的工艺条件：pH 值、底物与接种体比例、温度和初始乙醇浓度的影响。

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-11-07 DOI: 10.1007/s00284-024-03976-3

Victoria M Guzmán, Rodrigo J Leonardi, Sofía Racca, Raúl N Comelli

{"title":"Assessing Process Conditions on Xylose Fermentation in Spathaspora passalidarum: Effects of pH, Substrate-to-Inoculum Ratio, Temperature, and Initial Ethanol Concentration.","authors":"Victoria M Guzmán, Rodrigo J Leonardi, Sofía Racca, Raúl N Comelli","doi":"10.1007/s00284-024-03976-3","DOIUrl":"10.1007/s00284-024-03976-3","url":null,"abstract":"Bioethanol represents a clean and renewable alternative to fossil fuels, offering a significant reduction in environmental impact. Second-generation ethanol (2G) is produced using lignocellulosic biomass, which presents additional challenges due to the presence of hemicellulose. The pentose sugars within hemicellulose cannot be efficiently metabolized by conventional yeast strains like Saccharomyces cerevisiae. Consequently, the yeast Spathaspora passalidarum has emerged as a promising candidate for mixed fermentation processes, given its ability to utilize xylose. This study presents an in-depth metabolic, stoichiometric, and kinetic analysis of the fermentation performance of Sp. passalidarum NRRL Y-27907 in mixed glucose and xylose cultures. Emphasis was placed on examining variables from a novel perspective compared to existing literature. Specifically, the impacts of initial inoculum-substrate ratios, substrate composition, pH, temperature, and ethanol sensitivity were analyzed using a mathematical bioprocess approach. Sp. passalidarum NRRL Y-27907 exhibited sequential sugar consumption, with xylose being utilized only after glucose was exhausted. Ethanol yields in mixed cultures were comparable to those in individual-sugar cultures. The best fermentative performance was observed at 30 °C, with 25 g/L of xylose and an inoculum of 0.50 g/L. The strain exhibited significant robustness at pH 4.0 and was notably affected by initial ethanol concentrations up to 20 g/L. These findings provide crucial insights into the metabolic and fermentative behavior of Sp. passalidarum NRRL Y-27907, offering valuable information for the design of consolidated bioprocesses from lignocellulosic materials.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"81 12","pages":"448"},"PeriodicalIF":2.3,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142603679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whole-Genome Sequencing And Characterization Of Two Bacillus velezensis Strains from Termitarium and A Comprehensive Comparative Genomic Analysis of Biosynthetic Gene Clusters. 来自白蚁的两株 Velezensis 杆菌的全基因组测序和特性分析以及生物合成基因簇的综合比较基因组分析

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-11-07 DOI: 10.1007/s00284-024-03965-6

Venkatesan Dhanalakshmi, Jeyaprakash Rajendhran

{"title":"Whole-Genome Sequencing And Characterization Of Two Bacillus velezensis Strains from Termitarium and A Comprehensive Comparative Genomic Analysis of Biosynthetic Gene Clusters.","authors":"Venkatesan Dhanalakshmi, Jeyaprakash Rajendhran","doi":"10.1007/s00284-024-03965-6","DOIUrl":"10.1007/s00284-024-03965-6","url":null,"abstract":"The species Bacillus velezensis is known for its biosynthetic potential and metabolic versatility in producing several secondary metabolites and promoting plant growth. In this study, we isolated two B. velezensis strains, WGTg-8 and WGTm-299, from the termite gut and termitarium, which exhibited antimicrobial activity against multiple clinical and phytopathogens. The whole genomes of these strains were sequenced using the Illumina platform and annotated. The genome mining of the draft genome sequences revealed 48 biological gene clusters (BGCs) responsible for synthesizing various secondary metabolites. The construction of the similarity network of the BGCs and the comparative analysis with the genetically related organisms are aided in the identification of metabolites produced by these strains. We identified biosynthetic gene clusters (BGCs) coding for macrolactin H, bacilysin, bacillibactin, amylocyclin, comX4, and LCI, found in both strains with 100% similarity. The difficidin, bacillaene, thusin_alpha, and cericidin BGCs are exclusively found in strain WGTg-8, while the colicin BGC is exclusively present in the WGTm-299 strain. The fengycin and surfactin gene clusters are present in both strains with 80% similarity. Furthermore, 28 putative NRPS BGCs, NRPS-T1PKS hybrid clusters, a T1PKS, and a bacteriocin BGC were identified with very low similarity (≤ 25%) or no similarity with known antibiotics. In addition, we found several genes coding for plant growth-promoting properties, including nitrogen metabolism, hormone synthesis, sulfur metabolism, phosphate metabolism, and a few other properties.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"81 12","pages":"449"},"PeriodicalIF":2.3,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142603731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tetracycline and Oxacillin Act Synergistically on Biofilms and Display Increased Efficacy In Vivo Against Staphylococcus aureus. 四环素和奥沙西林可协同作用于生物膜，并在体内增强对金黄色葡萄球菌的疗效。

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-11-06 DOI: 10.1007/s00284-024-03959-4

Amy K Tooke, Rebecca E Hodges, Josie F Pyrah, Kenneth W Bayles, Stephen A Renshaw, Simon J Foster

{"title":"Tetracycline and Oxacillin Act Synergistically on Biofilms and Display Increased Efficacy In Vivo Against Staphylococcus aureus.","authors":"Amy K Tooke, Rebecca E Hodges, Josie F Pyrah, Kenneth W Bayles, Stephen A Renshaw, Simon J Foster","doi":"10.1007/s00284-024-03959-4","DOIUrl":"10.1007/s00284-024-03959-4","url":null,"abstract":"Oxacillin (bactericidal) and tetracycline (bacteriostatic) are clinically relevant antibiotics that are routinely prescribed to treat Staphylococcus aureus infections but not conventionally used in combination. There is an urgent need for treatment regimens that can act upon biofilms during infection, associated with chronic infections on indwelling devices, as well as acute planktonic (systemic) infection. Here we show that in an in vitro model oxacillin and tetracycline act synergistically against S. aureus UAMS-1 biofilms, reducing the concentration of both antibiotics necessary to eradicate an established biofilm. Using an in vivo zebrafish larval infection model with S. aureus NewHG, they display improved bacterial clearance compared to each drug alone and can counteract a loss of host phagocytes, an important innate defence against S. aureus. In these cases, the bacteriostatic nature of tetracycline enhances rather than dampens the bactericidal action of oxacillin, although an exact mechanism remains to be elucidated. We suggest a dual therapy could be of clinical use against biofilm-forming S. aureus and has a potential use in patients with a compromised immune system.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"81 12","pages":"447"},"PeriodicalIF":2.3,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11541413/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142589862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Host and Viral Factors Influencing Chronic Hepatitis B Infection Across Three Generations in a Family. 影响一个家庭三代人慢性乙型肝炎感染的宿主和病毒因素。

IF 2.3 3区生物学

Current Microbiology Pub Date : 2024-11-05 DOI: 10.1007/s00284-024-03963-8

Malihe Naderi, Seyed Masoud Hosseini, Naser Behnampour, Sima Besharat, Iraj Shahramian, Masoud Khoshnia, Abdolvahab Moradi

{"title":"Host and Viral Factors Influencing Chronic Hepatitis B Infection Across Three Generations in a Family.","authors":"Malihe Naderi, Seyed Masoud Hosseini, Naser Behnampour, Sima Besharat, Iraj Shahramian, Masoud Khoshnia, Abdolvahab Moradi","doi":"10.1007/s00284-024-03963-8","DOIUrl":"10.1007/s00284-024-03963-8","url":null,"abstract":"Chronic hepatitis B (CHB) infection is influenced by both virological and host factors. A total of 5,920 CHB patients were classified into four groups based on HBV seromarkers: three-generation families (CHB grandmother, mother, and child), two-generation families (CHB mother/child pairs), individuals recovered from HBV infection, and a control group. Serological markers, viral load, liver function tests (LFT), HBV mutations, HLA-DQ variations, cytokine polymorphisms, and liver stiffness measurements (LSM) were analyzed using FibroScan. Point mutations in genes such as core/pre-core (G1896A/G1899A), polymerase (H248N, H267Q, N263D), S (G145R, S143L), and X (C1500T, T1464C) were observed in 30% of three-generation pairs and 20% of two-generation pairs. The three-generation group exhibited the highest mean liver stiffness measurement (LSM) (4.94 ± 1.24 kPa), which is considered a predictor for the development of hepatocellular carcinoma (HCC). Subsequent HLA allele analysis identified HLA-DQB105:01 (OR = 0.27) as a risk factor for treatment resistance, while HLA-DQB105 (OR = 0.98), HLA-DQB103 (OR = 0.80), and HLA-DQB104:01 (OR = 0.70) were associated with HBV persistence in both three- and two-generation groups. Higher frequencies of specific polymorphisms, including G/G (TNF-α: 75%; IL-18: 74%), A/A (IL-10: 74.28%), and C/C (IL-1ß: 80%), were significantly linked to persistent infection. Analysis of viral sequences, HLA-DQB1 variations, cytokine polymorphisms, and genetic relationships within the phylogenetic tree revealed that 40% of CHB patients from three-generation families were infected by a shared source of transmission, as indicated by the presence of the same HBV genotype. This study underscores the complex interplay of host and viral factors that influence hepatitis B infection outcomes and suggests potential familial transmission pathways.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"81 12","pages":"446"},"PeriodicalIF":2.3,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142582254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0