Current Microbiology最新文献

{"title":"Effect of Chemical Sanitizers Alone or Combined with An Enzymatic Product Against Pseudomonas fluorescens Biofilms on Food-Grade Materials.","authors":"Francesco Blasi, Nicoletta Scaramuzza, Serena Chierici, Massimo Cigarini, Barbara Franceschini, Elettra Berni","doi":"10.1007/s00284-025-04485-7","DOIUrl":"https://doi.org/10.1007/s00284-025-04485-7","url":null,"abstract":"<p><p>In food industries microorganisms could resist to the sanitization processes routinely carried out, due to their peculiar ability to aggregate into biofilms, thus adhering to materials such as stainless steel (SS), poly-tetra-fluoro-ethylene (PTFE), hydroxyapatite, polystyrene, glass, or rubber. Since biofilms are a matter of concern for food producers, in this paper two sanitizers of common use in the food industry, respectively based on quaternary ammonium salts and alcohols, were tested alone or in combination with an enzymatic product against three different Pseudomonas fluorescens strains. Six-day-old biofilms grown on SS or PTFE were tested by applying different concentrations of each sanitizer at different treatment times, up to 10 min. When used in combination with the enzymatic product, sanitizers were applied after the disaggregating effect of the enzymatic product itself. The use of sanitizers without the enzymatic product allowed to achieve a total eradication of the biofilms only at high concentrations after long treatment times. The use of the enzymatic product combined with each sanitizer, at concentrations and treatment times that previously did not prove effective in eradicating Pseudomonas biofilms, allowed to register from 5 to 8 logarithmic reductions in the concentrations of the detached cells and, in some cases, their total inactivation. A better performance was obtained on SS compared to PTFE.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 11","pages":"512"},"PeriodicalIF":2.6,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145091323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coexpression of the Ectoine Biosynthetic Gene Cluster from Nocardiopsis dassonvillei NCIM 5124 in Escherichia coli by Using a Novel Strategy.","authors":"Pratik M Kadam, Vitthal T Barvkar, Swaranjali S Patil, Shubham B Ahirrao, Smita S Zinjarde","doi":"10.1007/s00284-025-04498-2","DOIUrl":"https://doi.org/10.1007/s00284-025-04498-2","url":null,"abstract":"<p><p>Ectoine, a natural cell protectant produced by a variety of bacteria has commercial value. In this study, Escherichia coli BL21(DE3) was engineered for producing ectoine by using a novel approach. The ectABC gene cluster from Nocardiopsis dassonvillei NCIM 5124 was codon optimized for E. coli and a pET-24a(+) based construct (pEctoineCAB) was designed. Two elements-P2A tag (derived from porcine teschovirus-1 that causes ribosomal skipping) and IRES sequence (internal ribosome entry site from encephalomyocarditis virus) were introduced to ensure sufficient expression levels of all the enzymes relevant to ectoine production. The ability of the transformant harbouring pEctoineCAB was evaluated for its ectoine biosynthetic capability. In the presence of 0.1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) as an inducer, 31.52 mg/g cell dry weight (CDW) of ectoine was obtained. After inclusion of 200 mM L-aspartic acid as precursor, this content was further enhanced to 131.41 mg/g CDW. With the acquired ability to produce ectoine, the recombinant strain tolerated sodium chloride (NaCl) up to a level of 5%. Compared to the original organism that produced 40.78 mg/g CDW of ectoine after 48 h in medium containing 5% NaCl, the recombinant produced higher content of ectoine (131.41 mg/g CDW) after 24 h of incubation in the absence of salt. On account of these features, the process of ectoine production by the recombinant was deemed effectual and economically lucrative for large-scale production. This is the first report on the use of P2A tag in E. coli for effective production of an economically important product.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 11","pages":"510"},"PeriodicalIF":2.6,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145079900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heavy Metal-Resistant Bacterial Communities in the Artsvanik Tailing Dump, Armenia.","authors":"Armine Margaryan, Sona Nikolyan, Irina Ayvazyan, Hovik Panosyan, Nils-Kåre Birkeland","doi":"10.1007/s00284-025-04488-4","DOIUrl":"https://doi.org/10.1007/s00284-025-04488-4","url":null,"abstract":"<p><p>Mining tailings contain high concentrations of toxic elements that can damage soil or aquatic ecosystems, reducing microbial diversity. To elucidate the bacterial community in tailings, we analyzed bacterial abundance in Cu and Mo mine tailing dump at the Artsvanik, via metagenomic and culturing. In terms of community structure, Illumina shotgun sequencing revealed that Actinomycetota exhibited the highest abundance (40.7%), followed by Pseudomonadota (22%), and Bacteroidota (10.75%). Acidobacteriota (5%), Chloroflexota (5%), Verrucomicrobia (4.25%), Planctomycetota (3.88%), Bacillota (2.8%), Gemmatimonadota (2%), and other phyla were present at low abundances. Metal-resistant bacteria in the genera Inquilinus, Methylobacterium, Sinorhizobium, Noviherbaspirillum, Variovorax, Pseudarthrobacter, Rheinheimera, Pseudomonas, Algoriphagus, Bacillus, and Niallia, were isolated from the soil and sludge samples and from weed plants in the surrounding area. For the isolated strains, the maximum tolerable concentrations of Ni(II), Cu(II), Zn(II), Cd(II), Co(II), Mo(VI), and Cr(VI) were 1-2, 1-4, 2-5, 0.3-0.6, 0.5-2, 60-68 and 0.2-0.6 mM, respectively. The strains grew in the presence of a combination of Ni(II), Cd(II), Zn(II), and Co(II) at 0.75 mM in growth medium. Owing to their high levels of resistance toward heavy metals, these isolated strains hold potential for use in bioremediation to reduce heavy-metal toxicity in contaminated environments.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 11","pages":"511"},"PeriodicalIF":2.6,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145085445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Stimulation Effect of Seaweed Polysaccharides on the Lower pH of the Salt-Tolerant Bacteria and Their Mechanism.","authors":"Juan Liu, Xiaolin Chen, Jiang Zhan, Song Liu, Xueqin Wang","doi":"10.1007/s00284-025-04484-8","DOIUrl":"https://doi.org/10.1007/s00284-025-04484-8","url":null,"abstract":"<p><p>Soda saline-alkali soil, as a kind of saline soil, damages soil physicochemical properties agricultural production. Therefore, the restoration of soda saline-alkali soil is of great significance to maintain food supply and ecological security. Among the restoration methods of soda saline-alkali soil, microbial remediation is favored due to its environmental friendliness. However, it is mostly associated with problems such as difficulty in colonization and low efficiency due to nutritional deficiency of soda saline-alkali soil. In this study seaweed polysaccharide was used as an inducer to investigate whether and how it affected screened bacteria from the sea mud to remediate soda saline-alkali soil. The results showed seaweed polysaccharides can promote the growth of salt-tolerant bacteria which made pH lower for the culture process. This was mainly because the seaweed polysaccharides especially Grateloupia filicina polysaccharide could promote the secretion of more organic acids such as citrulline, phenylpyruvic acid and D-phenyl lactic acid by salt-tolerant bacteria, and up-regulate the metabolic pathways such as phenylalanine metabolism, purine metabolism and so on, which can stimulate salt-tolerant bacteria to reduce the pH in the environment. Additionally, Grateloupia filicina polysaccharide could improve the biodiversity of microbial community, change the structure of microbial communities. This study provided a new efficient and eco-friendly method to better restore soda saline-alkali soil.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 11","pages":"508"},"PeriodicalIF":2.6,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145074646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the Genomic Landscape of Escherichia coli O1:K1:H7 ST59 in Non-complicated Urinary Infections from Colombia Through Whole-Genome Sequencing.","authors":"Andrés Felipe Cuspoca Orduz, Hernán David Roldán Hernandeza, Ravneet Kaur Grewal, Manik Prabhu Narsing Rao, Nury Nathalia Olaya-Galán, Yardani Rafael Méndez Fandiño, Luigi Cavallo, Mohit Chawla","doi":"10.1007/s00284-025-04500-x","DOIUrl":"10.1007/s00284-025-04500-x","url":null,"abstract":"<p><p>Escherichia coli (E. coli) is a Gram-negative bacterium known for causing both intestinal and extraintestinal infections in humans. Among extraintestinal infections, urinary tract infections (UTIs) are particularly prevalent and impactful. In Colombia, limited information is available regarding the molecular epidemiology of E. coli. This lack of data hinders the understanding of the local epidemiological landscape and the identification of pathogenic lineages that may contribute to public health concerns. This study aimed to characterize an E. coli strain isolated from a 24-year-old female patient with a community-acquired lower UTI, focusing on genotypic analysis through whole-genome shotgun sequencing (WGSS) and subsequent bioinformatics investigations. The identified strain belongs to phylogroup F, with serotype O1:H7 and sequence type (ST) 59. Several virulence factors, including traT and afimbrial adhesins (afaC), were identified, with afaC being notably uncommon in ST59 phylogroup F. In addition, an antibiotic susceptibility test was performed, and the isolate was found to be sensitive to all the antibiotics tested. This work contributes to the understanding of E. coli phylogroup F in Colombia and provides valuable genomic data, shedding light on the virulence profile of this strain in lower urinary tract infections.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 11","pages":"505"},"PeriodicalIF":2.6,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12443878/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145074670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Characterization of A Novel Pbunavirus with Promising Antibiofilm Activity Against Pseudomonas Aeruginosa.","authors":"Farnoosh Majlesain, Ashkan Abbasifard Semnani, Shamsozoha Abolmaali, Mehdi Sadeghi, Shakiba Darvish Alipour Astaneh","doi":"10.1007/s00284-025-04478-6","DOIUrl":"https://doi.org/10.1007/s00284-025-04478-6","url":null,"abstract":"<p><p>Endolysin, encoded by bacteriophages, has emerged as a promising candidate against antibiotic-resistant bacteria due to its ability to target bacterial cell walls. In this study, we isolated a new bacteriophage vB_PaeC_sem1 from sewage. The genome of vB_PaeC_sem1 is 66,420 bp, a double-stranded linear DNA that comprises 92 open reading frames (ORFs). Sem1 showed broad stability across various temperatures, pH, and chemical solutions. Heterologous expression of Lys N in Escherichia coli was done (LysN1). The antibacterial effects of Lys N1 were shown against Pseudomonas aeruginosa and its biofilm disruption. Recombinant LysN1 and LysN effectively lysed P. aeruginosa as well as, destroying up to 80% and 67% of bacterial biofilm (respectively). No toxic effects on A549 cells were detected. These results suggest Sem1 as a potential alternative for treating Gram-negative bacterial infections.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 11","pages":"507"},"PeriodicalIF":2.6,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145074592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic and Functional Characterization of Bacillus siamensis B01 with Antimicrobial Activity Against Vibrio spp.","authors":"Co Thi Kim Nguyen, Hoang Duc Nguyen","doi":"10.1007/s00284-025-04483-9","DOIUrl":"https://doi.org/10.1007/s00284-025-04483-9","url":null,"abstract":"<p><p>This study reports the first investigation of the biological, genomic, and anti-Vibrio properties of Bacillus siamensis B01 (= VTCC 910229) isolated from a mangrove forest in Vietnam. Comparative genomic analysis revealed a significant similarity between the genomes of strain B01 and B. siamensis KCTC 13613^T, as indicated by average nucleotide identity and digital DNA-DNA hybridization values of 98.8% and 97.5%, respectively. The draft genome comprised 3,823,156 bp, showed a GC composition of 46.2%, and encompassed 112 tRNAs and 3,738 coding sequences (CDSs), with key functional groups related to biological processes, amino acid transport, and metabolism. Within the genome, an 80,406 bp plasmid with a GC content of 38.5% was identified, carrying 105 CDSs, one tRNA, and seven mobile genetic elements without virulence and antibiotic resistance genes. Gene clusters for antibacterial compounds, including bacillaene, fengycin, difficidin, and bacillibactin, showed 100% similarity to known biosynthetic pathways. Pan-genome analysis revealed that strain B01 shares 2,433 core genes with 23 other B. siamensis genomes, whereas this isolate possesses 160 unique genes, accounting for 4.28% of its genome. The isolate grew optimally in neutral environments, tolerated alkaline pH and 10% NaCl, formed biofilms, showed no hemolysis, and secreted proteases, lipases, cellulases, and amylases. The ethyl acetate extract demonstrated antibacterial activity against Vibrio azureus, Vibrio sinaloensis, Vibrio neocaledonicus, Vibrio alginolyticus, and Vibrio parahaemolyticus with inhibition zones measuring 15.7-24.3 mm in diameter. These results underscore the promise of this Bacillus strain as a probiotic against pathogenic Vibrio in aquaculture.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 11","pages":"509"},"PeriodicalIF":2.6,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145074636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent Advances in Bioinoculant Formulations and Their Shelf-Life: A Review.","authors":"Selladurai Monica, Sivasakthivelan Panneerselvam, Raghu Rajasekaran, Sakthivel Nalliappan, Amudha Kailappan, Anandham Rangasamy","doi":"10.1007/s00284-025-04497-3","DOIUrl":"10.1007/s00284-025-04497-3","url":null,"abstract":"<p><p>Soil parameters, such as temperature, moisture, aeration, and nutrient composition, significantly affect plant growth and development. Agricultural methods currently depend on chemical fertilizers and pesticides to increase crop yields. However, excessive use of these fertilizers may lead to environmental pollution, soil deterioration, and the accumulation of heavy metals. Bioinoculants have emerged as promising solutions to address these challenges and promote sustainable agriculture. These beneficial microorganisms, including bacteria, fungi, and algae, can improve soil fertility, stimulate plant development, and increase nutrient uptake. Bioinoculants augment plant defense systems against pests and diseases, increasing agricultural yield while reducing reliance on chemical fertilizers. A key constraint impeding the utilization of bioinoculants is their restricted shelf life. Researchers have investigated several formulation processes and carrier materials to improve the survival rate of these bacteria. The longevity of bioinoculants can be extended by enhancing formulation techniques, selecting suitable carrier materials, and maintaining ideal storage conditions. This review thoroughly examines techniques, including carriers, adjuvants, stickers, and protectants, as well as smart delivery technologies, such as nanobiofertilizers, freeze-drying technology, biofilm formulation, rhizosphere engineered bioinoculants and direct seed injection techniques, to increase shelf life and effectiveness. Furthermore, the review discusses market challenges, international regulations, farmer incentives, and the role of public‒private partnerships in standardizing formulations. The integration of biofertilizers with regenerative agricultural practices and patent-related considerations are also examined. Overall, this review aims to provide a comprehensive understanding of strategies to increase the shelf life and performance of bioinoculants, facilitating their widespread application in sustainable agriculture.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 11","pages":"506"},"PeriodicalIF":2.6,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145074675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ruegeria discodermiae sp. nov., Ruegeria jejuensis sp. nov., and Ruegeria hyattellae sp. nov., Isolated from Marine Sponges.","authors":"Yea-Lin Moon, Kyung Hyun Kim, Ji-Yoon Shin, Jin-Sook Park","doi":"10.1007/s00284-025-04492-8","DOIUrl":"10.1007/s00284-025-04492-8","url":null,"abstract":"<p><p>Three Gram-stain-negative, strictly aerobic, cream-colored, catalase- and oxidase-positive bacterial strains, designated 2205SS24-7^T, 2304DJ69-11^T, and 2012CJ15-1^T, were isolated from marine sponges collected in the Republic of Korea. The three novel strains grew optimally at 25-28 °C, pH 7.0, and 2-3% (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene and draft genome sequences revealed that the three novel strains clustered together with Ruegeria spongiae 2012CJ41-6^T, forming a distinct clade within the genus Ruegeria. The 16S rRNA gene sequence similarities with R. spongiae were 97.6, 98.6, and 98.9%, respectively. Draft genome sequencing indicated genome sizes ranging from 5.2 to 6.1 Mb with DNA G+C contents between 59.0 and 60.5%. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values among the three novel strains ranged from 85.3-90.9% and 29.6-43.1%, respectively, indicating that each strain represents a separate species. Furthermore, ANI and dDDH values between the three novel strains and the type strains R. spongiae 2012CJ41-6^T, R. mediterranea M17^T, and R. litorea R-37^T were ≤ 90.9% and ≤ 43.1%, respectively, both below the proposed thresholds for species delineation. The three novel strains possessed ubiquinone-10 as the major isoprenoid quinone and summed feature 8 (C_18:1 ω7c and/or C_18:1 ω6c) as the major fatty acid. The major polar lipids identified were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and phosphatidylcholine. Taken together, these results support the proposal that strain 2205SS24-7^T (= KACC 23237^T = LMG 33064^T), 2304DJ69-11^T (= KACC 23805^T = MCCC 1K09534^T), and 2012CJ15-1^T (= KACC 23842^T = MCCC 1K09526^T) represent three novel species within the genus Ruegeria, for which the names Ruegeria discodermiae sp. nov., Ruegeria jejuensis sp. nov., and Ruegeria hyattellae sp. nov. are proposed, respectively.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 11","pages":"503"},"PeriodicalIF":2.6,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145069373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Roseobacter algicola sp. nov. and Sulfitobacter rhodophyticola sp. nov., Isolated from Marine Red Algae.","authors":"Seo Hui Choi, Woonhee Baek, Jeong Min Kim, Byeong Jun Choi, Hülya Bayburt, Jae Kyeong Lee, Sung Chul Lee, Che Ok Jeon","doi":"10.1007/s00284-025-04490-w","DOIUrl":"10.1007/s00284-025-04490-w","url":null,"abstract":"<p><p>Two Gram-stain-negative, catalase- and oxidase-positive, non-motile strains, S98ᵀ and JB4-11ᵀ, were isolated from marine red algae. Strain S98ᵀ was a strictly aerobic, ovoid to short rod-shaped bacterium that grew at 15-30 °C, pH 7.0-9.0, and 1.0-4.0% (w/v) NaCl. In contrast, strain JB4-11ᵀ was a facultatively anaerobic, rod-shaped bacterium that grew at 20-30 °C, pH 7.0-10.0, and 1.0-7.0% (w/v) NaCl. Both strains contained phosphatidylglycerol, phosphatidylcholine, two unidentified aminolipids, and an unidentified phospholipid as major polar lipids, with JB4-11ᵀ additionally possessing diphosphatidylglycerol. The predominant fatty acids were summed feature 8 (C_18:1 ω7c and/or C_18:1 ω6c) and C_16:0, and both strains contained ubiquinone-10 as the sole isoprenoid quinone. The genomic DNA G + C content was 60.0% for S98ᵀ and 60.6% for JB4-11ᵀ. Strains S98ᵀ and JB4-11ᵀ shared 97.3% 16S rRNA gene sequence similarity and formed distinct lineages from other Roseobacter and Sulfitobacter species, with ≤ 98.1% similarity, in 16S rRNA gene-based phylogenetic trees. Genome-based phylogenetic analyses revealed that strains S98ᵀ and JB4-11ᵀ clustered with Roseobacter cerasinus AI77ᵀ and Sulfitobacter aestuariivivens TSTF-M16ᵀ within the genera Roseobacter and Sulfitobacter, respectively. Average nucleotide identity and digital DNA-DNA hybridization values with other Roseobacter and Sulfitobacter species were below 75.9% and 19.7%, respectively. Based on phenotypic, chemotaxonomic, and genomic characteristics, strains S98ᵀ and JB4-11ᵀ represent novel species of Roseobacter and Sulfitobacter, for which the names Roseobacter algicola sp. nov. and Sulfitobacter rhodophyticola sp. nov. are proposed. The type strains are S98ᵀ (= KACC 23419ᵀ = JCM 36648ᵀ) and JB4-11ᵀ (= KACC 23867ᵀ = JCM 36646ᵀ), respectively.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 11","pages":"502"},"PeriodicalIF":2.6,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145069345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}