Current Microbiology最新文献

筛选
英文 中文
Chitinibacter mangrovi sp. nov., a Novel Chitin-Degrading Bacterium Isolated from Mangrove Sediment. 从红树林沉积物中分离的一种新型几丁质降解细菌——mangrovi sp. nov.
IF 2.3 3区 生物学
Current Microbiology Pub Date : 2025-05-21 DOI: 10.1007/s00284-025-04281-3
Yong Xu, Longzhi Chen, Liyan Yang, Lixia Pan, Mengting Zheng, Naikun Shen, Dengfeng Yang
{"title":"Chitinibacter mangrovi sp. nov., a Novel Chitin-Degrading Bacterium Isolated from Mangrove Sediment.","authors":"Yong Xu, Longzhi Chen, Liyan Yang, Lixia Pan, Mengting Zheng, Naikun Shen, Dengfeng Yang","doi":"10.1007/s00284-025-04281-3","DOIUrl":"10.1007/s00284-025-04281-3","url":null,"abstract":"<p><p>A bacterial strain with chitin-degrading ability, designated FCG-7<sup>T</sup>, was isolated from a mangrove sediment in Guangxi, PR China, and characterized using a polyphasic taxonomy approach. Cells of strain FCG-7<sup>T</sup> were Gram-stain-negative, aerobic, rod-shaped and formed milky-white colonies. Growth occurred at 15-37 °C (optimum, 30 °C), at pH 7-9 (optimum, pH 7-8) and with 0-1% NaCl (optimum, 0.5%). The major isoprenoid quinone was ubiquinone-8 (Q-8). The predominant fatty acids were summed feature 3 (C<sub>16:1</sub> ω6c and/or C<sub>16:1</sub>ω7c) and C<sub>16:0</sub>. The polar lipids comprised phosphatidylethanolamine, diphosphatidylglycerol, five unidentified phospholipids and two unidentified aminophospholipids. The G + C content of the genomic DNA was 53.8%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain FCG-7<sup>T</sup> belonged to the genus Chitinibacter and was closely related to Chitinibacter bivalviorum 2T18<sup>T</sup> (similarity 99.29%) and Chitinibacter fontanus STM-7<sup>T</sup> (similarity 98.85%). The average nucleotide identity (ANI) values of FCG-7<sup>T</sup> and the above two type strains were 81.4-83.1%, and the digital DNA-DNA hybridization (dDDH) values were 22.9-24.3%. Based on the phylogenetic, phenotypic, chemotaxonomic and genotypic characteristics, strain FCG-7<sup>T</sup> represents a novel species of the genus Chitinibacter, for which the name Chitinibacter mangrovi sp. nov. is proposed. The type strain is FCG-7<sup>T</sup> (= KCTC 8742<sup>T</sup> = GDMCC 1.4868<sup>T</sup>).</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"299"},"PeriodicalIF":2.3,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144110295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antimicrobial Resistant Pathogens in the Oral Cavity of White (Carcharodon carcharias), Bull (Carcharhinus leucas) and Tiger (Galeocerdo cuvier) Sharks from the East Coast of Australia. 澳大利亚东海岸白鲨(Carcharodon carcharias)、牛鲨(Carcharhinus leucas)和虎鲨(Galeocerdo cuvier)口腔中的抗微生物病原体。
IF 2.3 3区 生物学
Current Microbiology Pub Date : 2025-05-21 DOI: 10.1007/s00284-025-04272-4
Jessica McIntosh, Andrew Greenhill, Paul Butcher, Meagan Dewar
{"title":"Antimicrobial Resistant Pathogens in the Oral Cavity of White (Carcharodon carcharias), Bull (Carcharhinus leucas) and Tiger (Galeocerdo cuvier) Sharks from the East Coast of Australia.","authors":"Jessica McIntosh, Andrew Greenhill, Paul Butcher, Meagan Dewar","doi":"10.1007/s00284-025-04272-4","DOIUrl":"10.1007/s00284-025-04272-4","url":null,"abstract":"<p><p>Australia has one of the highest incidences of shark bites worldwide. Previous research on sharks has detected antibiotic-resistant pathogenic bacteria in the oral cavity, however, no such research has been conducted on species found in Australasian waters. In this study, 197 bacterial isolates were obtained from oral swabs taken from 153 sharks, including white (Carcharodon carcharias), tiger (Galeocerdo cuvier) and bull sharks (Carcharhinus leucas) caught on the east coast of Australia between May 2018 and April 2022. Isolates were identified using MALDI-TOF, and a disc diffusion assay was used to determine the antibiotic sensitivity of 72 isolates considered potential pathogens, including members of the genera Pseudomonas, Vibrio, Aeromonas, Enterobacter, Klebsiella and Psychrobacter. Resistance to one or more antibiotics was common, with 60%, 73%, and 74% of isolates from white, tiger and bull sharks resistant to one or more antibiotics, respectively. This research provides insights into which antibiotics are most suitable for treating shark bite wounds in eastern Australia, and possibly more broadly, to prevent infection. This study identified that over 51% of pathogens in the oral cavity of Australian sharks are resistant to one or more antibiotics, with most pathogens being resistant to commonly prescribed antibiotics. This research provides important information for medical professionals when it comes to treaty wounds inflicted by shark bites.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"300"},"PeriodicalIF":2.3,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12095389/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Wastewater and Clinical Based Epidemiology for Viral Surveillance in the Nile Delta of Egypt. 埃及尼罗河三角洲污水和基于临床的病毒监测流行病学。
IF 2.3 3区 生物学
Current Microbiology Pub Date : 2025-05-20 DOI: 10.1007/s00284-025-04267-1
Mohamed Nasr Fathi Shaheen, Nehal Ismail Ahmed, Elmahdy Mohamed Elmahdy
{"title":"Wastewater and Clinical Based Epidemiology for Viral Surveillance in the Nile Delta of Egypt.","authors":"Mohamed Nasr Fathi Shaheen, Nehal Ismail Ahmed, Elmahdy Mohamed Elmahdy","doi":"10.1007/s00284-025-04267-1","DOIUrl":"10.1007/s00284-025-04267-1","url":null,"abstract":"<p><p>The release of inadequately treated wastewater, containing human viruses, into the water environment or agricultural use represent a major problem in public health. In this study, SYBR Green-based real-time polymerase chain (qPCR) was applied to evaluate the prevalence of human polyomavirus (HPyV), papillomavirus (HPV), hepatitis A virus (HAV), and hepatitis E virus (HEV) in urban sewage and among children with acute gastroenteritis. The seasonal distribution in wastewater and viral removal by wastewater treatment process were also evaluated, over the 2 year sampling period. HPyV, HPV, HAV, and HEV were detected in 68%, 39.6% 42.4%, and 33.3% of the raw sewage, respectively, with highest incidence in winter season. In treated sewage samples, HPyV, HPV, HAV, and HEV were detected in 21%, 9.4%, 18.7%, and 0%, respectively. Furthermore, among the 200 diarrheal stool samples, HPyV, HPV, HAV, and HEV were detected in 72.5%, 50%, 13%, and 5%, respectively. HPyV was more prevalent in both environmental and clinical samples. The mean concentration of these viruses in raw sewage, treated sewage, and stool samples was 3.62 × 10<sup>6</sup> GC/ml, 4.03 × 10<sup>3</sup> GC/ml, and 4.05 × 10<sup>6</sup> GC/g, respectively. Examination of wastewater treatment process efficiency based on mean concentration values at entry and exit observed an overall reduction of 49.5%, 47.9%, 41.2%, 100%, for HPyV, HPV, HAV, and HEV, respectively. This study showed the benefit of environmental monitoring as an additional tool to investigate the epidemiology of these viruses circulating in a given community.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"296"},"PeriodicalIF":2.3,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Mechanistic Insight in Cr (VI) Bioremediation by Bacillus spp. SSAU-2 Under Multi-Heavy Metal Contamination. 多重重金属污染下芽孢杆菌sau -2对Cr (VI)的生物修复机制研究
IF 2.3 3区 生物学
Current Microbiology Pub Date : 2025-05-20 DOI: 10.1007/s00284-025-04274-2
Abhijeet Sharma, Manshi Agrawal, Akanksha Singh, Shanthy Sundaram, Saumya Jaiswal
{"title":"A Mechanistic Insight in Cr (VI) Bioremediation by Bacillus spp. SSAU-2 Under Multi-Heavy Metal Contamination.","authors":"Abhijeet Sharma, Manshi Agrawal, Akanksha Singh, Shanthy Sundaram, Saumya Jaiswal","doi":"10.1007/s00284-025-04274-2","DOIUrl":"10.1007/s00284-025-04274-2","url":null,"abstract":"<p><p>Climate change has significantly contributed to high level of contamination of multi-pollutants in the environment. For instance, it increases the intensity of rainfall, leading to soil erosion and leaching of heavy metals, pesticides and other pollutants into the water bodies. Additionally, climate change intensifies both natural processes and anthropogenic activities resulting in the widespread release and dispersal of heavy metals (HMs) and other pollutants, posing great risk to environment and human health. Cr (VI) is the most hazardous metal contaminant in the ecosystem. In the environment, it often coexists with other heavy metal such as Fe (III), Zn (II), Pb (II), Hg (II), Cd (II) and Cu (II) which interferes with the Cr (VI) removal strategies and significantly influence the efficiency of bioremediating microbes. In this study, the Cr (VI) removal potential of the novel microbe Bacillus sp. SSAU-2 was explored in the presence of multi-heavy metal contaminations in various combinations. The tolerance against HM of the SSAU-2 was analyzed with minimum inhibition concentration (MIC) study and the mechanism was observed by analyzing the various types pf Siderophore production in the presence of HM. Revealed that the SSAU-2 exhibits multi-heavy metal tolerance, with the following MIC order Pb (II) > Fe (III) > Cu (II) > Cr (VI) > Zn (II) > Cd (II) > Hg (II). The presence of Zn (II), Fe (III) and Cu (II) acted as positive inducers, enhancing its growth and Cr (VI) removal efficiency. Although SSAU-2 demonstrates remarkable resistance to most heavy metals, it is highly susceptible to Hg (II) and Cd (II). However, Hg (II) proved to be the most toxic, reducing the Cr (VI) removal efficiency from 83 to 32% even at a concentration of 0.1 ppm concentration. The mechanism underlying its multi-heavy metal tolerance is linked to the production of siderophore, particularly catechol-type siderophore. Thus, this study highlights the potential of SSAU-2 as a robust microbe, capable of sustaining its Cr (VI) removal ability even in the presence of multiple heavy metals. Therefore, this microbe can tackle the adverse climate change phenomenon and environmental pollution altogether.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"293"},"PeriodicalIF":2.3,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144110293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Viral Challenges in Wheat: Comprehensive Diagnosis and Innovative Management Approaches. 小麦的病毒挑战:综合诊断和创新管理方法。
IF 2.3 3区 生物学
Current Microbiology Pub Date : 2025-05-20 DOI: 10.1007/s00284-025-04280-4
Tanu, Vikas Kumar Singh, Abhishek Pandey, Vijay Gahlaut, Abhijit Kumar
{"title":"Viral Challenges in Wheat: Comprehensive Diagnosis and Innovative Management Approaches.","authors":"Tanu, Vikas Kumar Singh, Abhishek Pandey, Vijay Gahlaut, Abhijit Kumar","doi":"10.1007/s00284-025-04280-4","DOIUrl":"10.1007/s00284-025-04280-4","url":null,"abstract":"<p><p>Wheat (Triticum aestivum L.) is a staple crop cultivated globally, providing essential nutrition to billions. However, viral diseases pose a major challenge, causing yield losses of up to 80% and significant economic damage. More than 50 distinct viruses infect wheat, with key threats including wheat streak mosaic virus (WSMV), barley yellow dwarf viruses (BYDVs), high plains wheat mosaic virus (HPWMoV), soilborne wheat mosaic virus (SBWMV), and wheat yellow mosaic virus (WYMV). These viruses are primarily transmitted by vectors such as wheat curl mites (Aceria tosichella), aphids, and soilborne fungi like Polymyxa graminis. Diagnosing wheat viral infections is challenging due to overlapping symptoms with other plant stresses and the microscopic nature of viruses. Recent advancements in molecular and serological diagnostics, including Enzyme-Linked Immunosorbent Assay (ELISA), RT-Polymerase Chain Reaction (RT-PCR), CRISPR-based detection, and biosensors, have improved virus identification and monitoring. Since chemical treatments are ineffective, integrated management strategies such as breeding virus-resistant varieties, controlling vector populations, adjusting cultural practices, and utilizing RNA interference (RNAi) are essential for mitigating disease spread. This review provides a comprehensive analysis of wheat viral diseases, focusing on cutting-edge diagnostic tools and sustainable management approaches. By addressing knowledge gaps and highlighting future research directions, it aims to support the development of virus-resistant wheat, ensuring global food security.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"294"},"PeriodicalIF":2.3,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Critical Review on the Potential of Inactivated Bacteria in Counteracting Human Pathogens. 灭活细菌在对抗人类病原体方面的潜力综述。
IF 2.3 3区 生物学
Current Microbiology Pub Date : 2025-05-20 DOI: 10.1007/s00284-025-04282-2
Aidin Lalezadeh, Manouchehr Fadaee, Samira Saedi, Javad Nezhadi, Mahdi Asghari Ozma, Somayeh Ahmadi, Mahdi Mobaseri, Hossein Samadi Kafil
{"title":"A Critical Review on the Potential of Inactivated Bacteria in Counteracting Human Pathogens.","authors":"Aidin Lalezadeh, Manouchehr Fadaee, Samira Saedi, Javad Nezhadi, Mahdi Asghari Ozma, Somayeh Ahmadi, Mahdi Mobaseri, Hossein Samadi Kafil","doi":"10.1007/s00284-025-04282-2","DOIUrl":"10.1007/s00284-025-04282-2","url":null,"abstract":"<p><p>Bacterial infections are a major global public health challenge, especially with increasing antibiotic resistance. Postbiotics, bioactive compounds produced by probiotics, have been proposed as a novel strategy to inhibit the growth of pathogenic bacteria and address antibiotic resistance. Similar to probiotics and certain food ingredients, postbiotics can also modulate beneficial microbial communities and ultimately contribute to host health. Postbiotics derived from probiotics may affect the physical and chemical conditions of the intestinal environment, and by enhancing the host's immune system, directly interfere with the metabolic pathways and signaling of pathogenic bacteria. Postbiotics inhibit biofilm formation, reduce the expression of antibiotic resistance genes, and enhance the efficacy of antibiotic therapies. They are effective against resistant bacteria such as Escherichia coli and Clostridium difficile and reduce the risk of dental infections caused by Streptococcus mutans. Some postbiotics, such as lactic acid and antimicrobial peptides derived from Lactobacillus and Bifidobacterium genus, help the immune system dealing resistant bacteria such as Pseudomonas aeruginosa, Staphylococcus aureus, and Helicobacter pylori. The review investigates the mechanisms of action and applications of postbiotics in the control of pathogenic bacteria and their role as a complement to existing treatments.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"295"},"PeriodicalIF":2.3,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144110291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Loop 3 Repeats in Omp34 is a Promising Immunogen for Vaccine Development Against Acinetobacter baumannii Infections. Omp34中的环3重复序列是开发抗鲍曼不动杆菌感染疫苗的有希望的免疫原。
IF 2.3 3区 生物学
Current Microbiology Pub Date : 2025-05-19 DOI: 10.1007/s00284-025-04275-1
Roghayeh Bashiri, Abolfazl Jahangiri, Saeede Masoomkhani, Iraj Rasooli
{"title":"Loop 3 Repeats in Omp34 is a Promising Immunogen for Vaccine Development Against Acinetobacter baumannii Infections.","authors":"Roghayeh Bashiri, Abolfazl Jahangiri, Saeede Masoomkhani, Iraj Rasooli","doi":"10.1007/s00284-025-04275-1","DOIUrl":"10.1007/s00284-025-04275-1","url":null,"abstract":"<p><p>The overuse of antibiotics has led to antibiotic-resistant Acinetobacter baumannii. This study evaluated the protective effect of a recombinant protein, derived from Omp34's loop 3 repeats, as a potential vaccine candidate against A. baumannii in a murine sepsis model. Recombinant Omp34 and L3 × 5 proteins were expressed in E. coli BL21 using the autoinduction method. The proteins were then purified and verified. To assess their protective efficacy, the proteins were injected into BALB/c mice, and the vaccine's effectiveness in both prevention and treatment stages was evaluated in a murine sepsis model against an infection caused by a clinical strain of A. baumannii. The study shows that recombinant proteins Omp34 and Omp34L3 × 5 have protective and immunogenic properties against A. baumannii. Using the immunogen loop 3, rather than the mature Omp34 protein, to stimulate the immune system yielded promising results. Omp34 offered superior protection, likely through B- and T-cell activation, while L3 × 5, though less protective, induced significant antibody production and recognized the clinical strain, warranting further research. The study highlights the potential of recombinant proteins Omp34 and Omp34L3 × 5 as vaccine candidates against A. baumannii. Omp34 provided stronger protection in mouse sepsis models, likely due to its activation of both B- and T-cell responses. While Omp34L3 × 5 was less protective, it still produced significant antibodies and recognized clinical strains. The results emphasize the need for further research into these proteins to develop vaccines or treatments against A. baumannii infections.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"292"},"PeriodicalIF":2.3,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144101144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genomic Features of E. ruysiae Associated with the Ecological Origin: Implications for the Environmental Hypothesis. 与生态起源相关的红叶蝉基因组特征:对环境假说的启示。
IF 2.3 3区 生物学
Current Microbiology Pub Date : 2025-05-17 DOI: 10.1007/s00284-025-04268-0
Martín Saraceno, Nicolás Frankel, Martín Graziano
{"title":"Genomic Features of E. ruysiae Associated with the Ecological Origin: Implications for the Environmental Hypothesis.","authors":"Martín Saraceno, Nicolás Frankel, Martín Graziano","doi":"10.1007/s00284-025-04268-0","DOIUrl":"10.1007/s00284-025-04268-0","url":null,"abstract":"<p><p>Escherichia cryptic clades represent a relatively unexplored taxonomic cluster believed to exhibit characteristics associated with a free-living lifestyle, which is known as the environmental hypothesis. This hypothesis suggests that certain Escherichia strains harbour traits that favour their environmental persistence, thus expanding the ecological commensal niche of the genus. While surveying Escherichia diversity in an urban South American stream we isolated the first environmental cryptic clade IV strain in South America (339_SF). Here we report the genomic characterization of 339_SF strain in the context of existing genomic information for E. ruysiae (cryptic clades III and IV). A comparative analysis of genomes within the same species stemming from diverse ecological sources and geographical locations reveals close phylogenetic proximity between our isolate and strains of environmental origin. Based on genetic content, we observed two clusters associated with the environmental source within E. ruysiae. In addition, we identified genes relatively more represented in the environmental strains: genes associated with carbohydrate metabolism (ydjG), stress response and DNA damage repair (such as umuD, higA and yddM). On the other hand, the gene rrrQ, associated with defense against other microorganisms, was significatively enriched in genomes of commensal origin. Our findings suggest that genomic features within E. ruysiae favoring its persistence in open environments may have arisen more than once, with these events being associated with the use of alternative energy sources and the resistance to various stressors specific to these environments.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"291"},"PeriodicalIF":2.3,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bioremediation Performance of Recombinant Shewanella azerbaijanica; Considering Uranium Removal in the Presence of Nitrate. 重组阿塞拜疆希瓦氏菌的生物修复性能研究考虑硝酸盐存在下铀的去除。
IF 2.3 3区 生物学
Current Microbiology Pub Date : 2025-05-17 DOI: 10.1007/s00284-025-04228-8
Razieh Ghasemi, Faezeh Fatemi, Elham Rastkhah
{"title":"Bioremediation Performance of Recombinant Shewanella azerbaijanica; Considering Uranium Removal in the Presence of Nitrate.","authors":"Razieh Ghasemi, Faezeh Fatemi, Elham Rastkhah","doi":"10.1007/s00284-025-04228-8","DOIUrl":"10.1007/s00284-025-04228-8","url":null,"abstract":"<p><p>Genetic engineering in microorganisms has emerged as a promising approach for pollutant removal from industrial wastewater. Shewanella azerbaijanica has the ability to reduce uranium. This study examined the impact of high-nitrate concentrations on uranium bioreduction in both native and recombinant bacterial strains. Bacterial performance was evaluated in terms of uranium bioreduction (measured via ICP-AES method), and survival in anaerobic conditions (measured via Neubauer chamber counting) in the presence of uranium and nitrate over various time intervals (24 h, 1 week, 4 weeks, 4 months, and 9 months). Although the recombinant strain showed a lower cell population than the wild-type strain, it achieved 20% higher uranium reduction after 24 h of incubation in uranium and nitrate-containing conditions. This suggests that the genetic modifications enhanced extracellular electron transfer (EET). The improved bioremediation efficiency may be attributed to the cloned mtrC gene, which promotes more effective electron transfer in Shewanella bacteria. Additionally, uranium removal may have been further enhancedby the inactivation of the napB gene using the SDM method. This high-performance trends was consistent across all time intervals. In wild-type S. azerbaijanica uranium removal rates were74%, 54%, 96 and 99% after 1 week, 4 weeks, 4 months, and 9 months, respectively. Inrecombinant bacteria, these rates increased to 91%, 78%, 96%, and 100% at the same time points. The bioreduction mechanism was further confirmed by X-ray diffraction (XRD) analysis, which verified the ability of S. azerbaijanica to reduce uranium in the presence of nitrate. Overall, this study identifies the recombinant bacterium as promising candidate for future metal bioreduction research.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"290"},"PeriodicalIF":2.3,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Multifunctional Silver Nanoparticles Synthesized via Exiguobacterium aurantiacum: Applications in Dye Remediation, Anticancer and Antibacterial Activity. 金离合杆菌合成多功能银纳米粒子:在染料修复、抗癌和抗菌活性中的应用。
IF 2.3 3区 生物学
Current Microbiology Pub Date : 2025-05-15 DOI: 10.1007/s00284-025-04259-1
Vishal Prajapati, Kuldeep Luhana, Bhakti Patel, Disha Patel, Santosh Kumar Sahu, Snehal Bagatharia, Nisha Choudhary, Virendra Kumar Yadav, Ashish Patel
{"title":"Multifunctional Silver Nanoparticles Synthesized via Exiguobacterium aurantiacum: Applications in Dye Remediation, Anticancer and Antibacterial Activity.","authors":"Vishal Prajapati, Kuldeep Luhana, Bhakti Patel, Disha Patel, Santosh Kumar Sahu, Snehal Bagatharia, Nisha Choudhary, Virendra Kumar Yadav, Ashish Patel","doi":"10.1007/s00284-025-04259-1","DOIUrl":"10.1007/s00284-025-04259-1","url":null,"abstract":"<p><p>The study investigates adaptations of photovoltaic solar panel isolates and their applications. Exiguobacterium aurantiacum (KKOHNGU1) was extracted from a PV solar panel in Patan, Gujarat, India. The objective was to synthesize and characterize silver nanoparticles (AgNPs) using E. aurantiacum (KKOHNGU1) to evaluate their potential in environmental remediation, anticancer, and antibacterial activities. MALDI-TOF analysis identified secondary metabolites as stabilizing and reducing agents. AgNPs were characterized using UV-visible spectroscopy (peak at 425 nm), X-ray diffraction (peaks at 27.6°, 32.12°, and 46.06°), Fourier transform infrared spectroscopy field emission scanning electron microscopy and energy-dispersive spectroscopy, shows spherical crystalline AgNPs with a mean size (121.44 nm). AgNPs (15 mg/mL) showed antibacterial activities, especially against S. aureus, with a 17.5 mm zone of inhibition MIC (2-32 µg/mL), and MBC (32-150 µg/mL). The MTT assay revealed anticancer activity of AgNPs on the MDA-MB-231 breast cancer cell line with an IC<sub>50</sub> value of 172.96 µg/mL. A comparative study showed both bacteria and AgNPs had high potential for MB dye removal, with bacteria achieving 73.55% removal within 120 h and AgNPs showing 97.54% removal within 10 min. These results indicate E. aurantiacum and AgNPs have potential for environmental remediation, particularly in wastewater treatment.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 7","pages":"289"},"PeriodicalIF":2.3,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信