Current Microbiology最新文献_第6页

Intrasporangium zincisolvens sp. nov., A Novel Actinobacterium Isolated from Rhizosphere Soil. 一种从根际土壤分离的放线菌。

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-01-16 DOI: 10.1007/s00284-025-04073-9

Lu-Yao Feng, Jiang-Yuan Zhao, Zhu-Feng Shi, Ming-Gang Li, Te Pu, Zhu-Li Shi, Xin Liu, Yu Wang, Le-Le Li, Pei-Wen Yang

{"title":"Intrasporangium zincisolvens sp. nov., A Novel Actinobacterium Isolated from Rhizosphere Soil.","authors":"Lu-Yao Feng, Jiang-Yuan Zhao, Zhu-Feng Shi, Ming-Gang Li, Te Pu, Zhu-Li Shi, Xin Liu, Yu Wang, Le-Le Li, Pei-Wen Yang","doi":"10.1007/s00284-025-04073-9","DOIUrl":"10.1007/s00284-025-04073-9","url":null,"abstract":"A novel phosphate-solubilizing and zinc-solubilizing actinobacterium strain YIM S08009T was isolated from rhizosphere soil collected from Pinus yunnanensis in Wuliangshan National Nature Reserve, Pu'er City, Yunnan Province, southwest PR China. Cells of strain YIM S08009T were Gram-stain-positive, non-motile, irregular rods to cocci, and formed yellow and white colonies on nutrient agar. Growth was observed at 10-40 °C (optimum 25-35 °C), pH 6.0-8.5 (optimum 7), and 0-4% (w/v) NaCl (optimum 1%). The cell wall peptidoglycan contained LL-diaminopimelic acid. The whole-cell sugars were mannose, ribose, glucose, and galactose. The predominant menaquinone was MK-8(H4). Major polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, unknown lipid, and 3 unknown phospholipids. The predominant fatty acids were iso-C14:0, iso-C15:0, and iso-C16:0. The DNA G + C content was 72.6%. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM S08009T belonged to genus Intrasporangium, and was most closely related to Intrasporangium flavum MUSC 78T, with 99.0% 16S rRNA gene sequence similarity. Strain YIM S08009T shared 90.1% orthologous average nucleotide identity (OrthoANI) and 39.8% digital DNA-DNA hybridization (dDDH) with I. flavum MUSC 78T. The genome of strain YIM S08009T contained phosphate-solubilizing genes (SenX3, RegX3, pstSCAB, ugpBAEC, phoA) and zinc-solubilizing genes (znuABC, zupT), and the strain had also demonstrated in vitro phosphorus and zinc solubilization. Based on the genotypic and phenotypic analyses, strain YIM S08009T (= CGMCC 1.60168T = NBRC 116604T = KCTC 59021T) represents a novel Intrasporangium species, for which the name Intrasporangium zincisolvens sp. nov. is proposed.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 2","pages":"87"},"PeriodicalIF":2.3,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial and Cytotoxic Potential of Endophytic Aspergillus versicolor Isolate from the Medicinal Plant Plectranthus amboinicus. 药用植物 Plectranthus amboinicus 内生曲霉分离物的抗菌和细胞毒性潜力

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-01-15 DOI: 10.1007/s00284-024-04050-8

S Manaswini, R Akshata, V Bhoomika, P Nandini, K Ganapathy, K P Deeshma

{"title":"Antimicrobial and Cytotoxic Potential of Endophytic Aspergillus versicolor Isolate from the Medicinal Plant Plectranthus amboinicus.","authors":"S Manaswini, R Akshata, V Bhoomika, P Nandini, K Ganapathy, K P Deeshma","doi":"10.1007/s00284-024-04050-8","DOIUrl":"10.1007/s00284-024-04050-8","url":null,"abstract":"Endophytic fungi are non-pathogenic organisms that colonise healthy plant tissues asymptomatically. Endophytes derived from medicinal plants are sources for identifying natural products and bioactive compounds with potential uses for industry, medicine, agriculture, and related sectors. In the present study, ethyl acetate crude extracts of four endophytic fungal isolates (CALF1, CALF4, and CASF1) from the medicinal plant Plectranthus amboinicus showed potent antimicrobial activity against the test pathogenic bacteria Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Bacillus subtilis using disc diffusion assays. A colorimetric microdilution assay to detect the minimum inhibitory concentration (MIC) revealed that the extracellular extract (ECE) of CASF1 isolate had the lowest MIC values against the test pathogenic bacteria (0.19-6.25 mg/ml) compared to other CALF1 and CALF4. Cytotoxic activity of CASF1-ECE against the drug-resistant KB.CHR.8-5 cancer cell line tested by the MTT assay showed complete cell death at a concentration of 220 μg/mL and the half-maximum inhibitory concentration (IC50) was determined to be 77.9 ± 09 μg/mL. GC-MS analysis showed hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl) ethyl ester, as the dominant compound among the bioactive compounds identified in the EXE of CASF1 isolate, with the highest peak in the GC chromatogram, indicating its role in the antimicrobial and cytotoxic activity of CASF1. Molecular identification of CASF1 using 18S rRNA sequencing and BLAST analysis detected CASF1 as an isolate of Aspergillus versicolor with 100% sequence identity.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 2","pages":"84"},"PeriodicalIF":2.3,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142983057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity and Plant Growth-Promoting Activities of Culturable Seed Endophytes in Abies pindrow (Royle ex D. Don) Royle: Their Role in Seed Germination and Seedling Growth. 冷杉（Abies pindrow）（Royle ex D. Don） Royle可培养种子内生菌的多样性及其在种子萌发和幼苗生长中的作用。

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-01-13 DOI: 10.1007/s00284-025-04062-y

Iflah Rafiq, Zafar Ahmad Reshi, Iqra Bashir

{"title":"Diversity and Plant Growth-Promoting Activities of Culturable Seed Endophytes in Abies pindrow (Royle ex D. Don) Royle: Their Role in Seed Germination and Seedling Growth.","authors":"Iflah Rafiq, Zafar Ahmad Reshi, Iqra Bashir","doi":"10.1007/s00284-025-04062-y","DOIUrl":"10.1007/s00284-025-04062-y","url":null,"abstract":"Abies pindrow, a vital conifer in the Kashmir Himalayan forests, faces threats from low regeneration rates, deforestation, grazing, and climate change, highlighting the urgency for restoration efforts. In this context, we investigated the diversity of potential culturable seed endophytes in A. pindrow, assessed their plant growth-promoting (PGP) activities, and their impact on seed germination and seedling growth. We cultured 729 microbial isolates that were resolved into 30 bacterial and 18 fungal species across various phyla. All 48 isolates exhibited various PGP activities. Specifically, all the cultured isolates showed IAA activity with concentrations ranging from 2.07 to 8.453 μg/ml, while ammonia production ranged from 0.936 to 3.436 mM/ml. Only 18 isolates, predominantly fungi, tested positive for phosphate solubilisation. Additionally, 20 isolates exhibited the ability to inhibit the growth of Fusarium oxysporum f.sp. pini. We selected four bacterial and six fungal isolates, which showed positive results for all PGP activities, to evaluate their effects on seed germination and seedling growth. Notably, seed germination rates increased by 750.9% under bacterial and consortium treatments and by 550.45% under fungal treatment. The consortium treatment also led to a 96% increase in needle count, while bacterial treatment enhanced stem length by 55.4%. Furthermore, shoot biomass also showed a significant increase with both bacterial and fungal treatments, underscoring the potential of harnessing seed endophytes to boost A. pindrow seedling health and resilience. This study underscores the crucial role of seed endophytic diversity in enhancing seed germination, seedling growth, and forest restoration efforts.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 2","pages":"82"},"PeriodicalIF":2.3,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142970039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome Analysis of Coxsackievirus A4 Identified from Herpangina Children in Northern China. 中国北方赫氏咽峡病患儿柯萨奇病毒A4的基因组分析

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-01-13 DOI: 10.1007/s00284-025-04075-7

Tiegang Lv, Yingming Li, Pengfei You, Xiaoyan Chen, Huafeng Li, Na Zhang, Chunling Ma, Yanli Zhang, Jie Chen

{"title":"Genome Analysis of Coxsackievirus A4 Identified from Herpangina Children in Northern China.","authors":"Tiegang Lv, Yingming Li, Pengfei You, Xiaoyan Chen, Huafeng Li, Na Zhang, Chunling Ma, Yanli Zhang, Jie Chen","doi":"10.1007/s00284-025-04075-7","DOIUrl":"10.1007/s00284-025-04075-7","url":null,"abstract":"Sporadic epidemics of coxsackievirus A4 (CVA4) have been reported worldwide. However, the lack of the whole genome sequence has restricted the study of the gene characterization and evolution of CVA4. In this study, four whole genome sequences and 17 VP1 sequences of CVA4 identified from Linyi, northern China, in summer 2024 were used for genetic characterization and phylogenetic analysis. Four genotypes (A, B, C, and D) and five subgenotypes (C1-C5) were identified based on VP1 sequences. The Linyi CVA4 strains belong to subgenotype C2, which has also been the main prevalent subgenotype in China in recent years. The Linyi CVA4 strains exhibited high homology with the CVA4 prototype strain in the P1 region while exhibited higher homology with some non-CVA4 EV-A strains identified in China, including five CVA2 strains, three CVA5 strains, three CVA6 strains, one CVA8 strain, one CVA12 strain, and one CVA14 strain in the P2 and P3 regions. Recombination analysis of the whole genome sequences of the Linyi CV4 strains revealed that two Linyi CVA4 strains might be recombinants of one Shanghai CVA4 strain (KJ541163) and one Jiangsu CVA2 strain (OL519580). One Linyi CVA4 strain might be a recombinant of one Shandong CVA2 strain (MK967660) and one Shanghai CVA4 strain (KJ541163).","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 2","pages":"83"},"PeriodicalIF":2.3,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142977910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Different Preservation Methods on the Structure and Diversity of Intestinal Microbiota of Marine Fishes. 不同保存方法对海洋鱼类肠道菌群结构和多样性的影响。

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-01-13 DOI: 10.1007/s00284-025-04060-0

Xueqing Du, Jing Zhao, Lisheng Wu, Shaoxiong Ding

{"title":"Effects of Different Preservation Methods on the Structure and Diversity of Intestinal Microbiota of Marine Fishes.","authors":"Xueqing Du, Jing Zhao, Lisheng Wu, Shaoxiong Ding","doi":"10.1007/s00284-025-04060-0","DOIUrl":"10.1007/s00284-025-04060-0","url":null,"abstract":"The fish intestine is a complex ecosystem where microbial communities are dynamic and influenced by various factors. Preservation conditions during field collection can introduce biases affecting the microbiota amplified during sequencing. Therefore, establishing effective, standardized methods for sampling fish intestinal microbiota is crucial. This study used hybrid groupers (Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂) to examine the effects of six preservation methods: dry ice (1 day), dry ice (1 day) followed by - 80 °C storage (5 days), liquid nitrogen (1 day), liquid nitrogen (1 day) with subsequent - 80 °C storage (5 days), refrigeration at 4 °C (3 days), and freezing at - 20 °C (3 days), with fresh samples as controls. High-throughput 16S rRNA sequencing assessed microbial diversity, community structure, dominant species, and OTU abundance across treatments. Results indicated that dry ice and liquid nitrogen methods, especially with - 80 °C storage, had minimal impact on microbial diversity and structure. Compared to other preservation methods, refrigeration at 4 °C and freezing at - 20 °C may result in suboptimal reproducibility and altered community structure, particularly affecting rare microbial taxa. This study underscores the need for standardized preservation techniques to ensure accurate fish intestinal microbiota analysis and provides a foundation for future research.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 2","pages":"81"},"PeriodicalIF":2.3,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142970047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human Brucellosis in the Rural and Urban Population of Pakistan: Seroprevalence, Risk Factors, and Clinical Manifestations. 巴基斯坦农村和城市人口中的人类布鲁氏菌病：血清阳性率、危险因素和临床表现

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-01-11 DOI: 10.1007/s00284-025-04063-x

Faisal Abbas, Shahzad Ali, Ali Muhammad, Asima Azam, Amira A Moawad, Mohammad Ejaz, Anam Iftikhar, Maryam Dadar

{"title":"Human Brucellosis in the Rural and Urban Population of Pakistan: Seroprevalence, Risk Factors, and Clinical Manifestations.","authors":"Faisal Abbas, Shahzad Ali, Ali Muhammad, Asima Azam, Amira A Moawad, Mohammad Ejaz, Anam Iftikhar, Maryam Dadar","doi":"10.1007/s00284-025-04063-x","DOIUrl":"10.1007/s00284-025-04063-x","url":null,"abstract":"Brucella spp. is the bacterium responsible for brucellosis, a zoonotic infection that affects humans. This disease poses significant health challenges and contributes to poverty, particularly in developing countries. This study aimed to assess the seroprevalence, risk factors, and clinical symptoms of human brucellosis within the general population of Multan and Muzaffargarh, Pakistan. A total of 307 blood samples were collected from patients visiting local hospitals in Multan and Muzaffargarh between August 2015 and January 2016. Demographic information, risk factors, and clinical outcomes were documented. Serum samples were initially screened for anti-Brucella antibodies using the Rose Bengal Plate Test, and positive cases were subsequently confirmed through RT-PCR. The chi-square test assessed the link between Brucella positivity and the identified risk factors. The study recorded an overall seroprevalence of 6.8%, with 8.9% in Multan and 4.3% in Muzaffargarh. Genus-specific Brucella detection through RT-PCR confirmed that 20 out of 21 samples were positive. Significant associations with human brucellosis were found for contact with aborted animals (p = 0.032) and consumption of raw milk (p = 0.031), while factors such as age, gender, occupation, urbanicity, and geographical region did not show a significant impact on seropositivity (p > 0.05). Non-specific clinical symptoms were commonly observed among seropositive patients. The findings highlight the significance of close human interaction with infected animals, especially concerning livestock practices and dairy product consumption. The results also emphasize the importance of focusing efforts on raising awareness in risky occupations and developing control programs by healthcare authorities.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 2","pages":"80"},"PeriodicalIF":2.3,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142964204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Fungal Symbionts of Co-occurring Orchids on the Seed Germination of Serapias orientalis and Spiranthes spiralis. 共生兰花真菌共生体对东方七翼草和螺旋线草种子萌发的影响。

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-01-10 DOI: 10.1007/s00284-024-04055-3

Ines Harzli, Yasemin Özdener Kömpe

{"title":"Impact of Fungal Symbionts of Co-occurring Orchids on the Seed Germination of Serapias orientalis and Spiranthes spiralis.","authors":"Ines Harzli, Yasemin Özdener Kömpe","doi":"10.1007/s00284-024-04055-3","DOIUrl":"10.1007/s00284-024-04055-3","url":null,"abstract":"Interactions with mycorrhizal fungi are increasingly recognized as crucial ecological factors influencing orchids' distribution and local abundance. While some orchid species interact with multiple fungal partners, others show selectivity in their mycorrhizal associations. Additionally, orchids that share the same habitat often form relationships with different fungal partners, possibly to reduce competition and ensure stable coexistence. However, the direct impact of variations in mycorrhizal partners on seed germination remains largely unknown. We examined how fungal associates' specific identity and origin affect seed germination in Spiranthes spiralis and Serapias orientalis through in situ symbiotic germination experiments. A total of four fungal isolates, Tulasnellaceae and Ceratobasidiaceae were successfully isolated and cultured from S. spiralis and S. orientalis and two additional orchid species found in the same habitat: Neotinea tridentata and Orchis provincialis. While all fungal strains facilitated the swelling of seed embryos, only the fungal associate, a member of the Ceratobasidiaceae family isolated from N. tridentata, (NT2) was capable of inducing protocorm formation and subsequent seedling growth of S. spiralis seeds. Another fungal associate belonging to the Tulasnellaceae family and isolated from O. provincialis (OP3) supported seed germination up to the seedling stage of S. orientalis seeds. However, the remaining two fungal strains did not support seed germination. We conclude that fungal associates of co-occurring orchids can promote seed germination and seedling growth in S. spiralis and S. orientalis.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 2","pages":"79"},"PeriodicalIF":2.3,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142946389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ribotyping Staphylococcus epidermidis Using Probabilistic Sequence Analysis and Levenshtein Distance Algorithm. 基于概率序列分析和Levenshtein距离算法的表皮葡萄球菌核分型研究。

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-01-10 DOI: 10.1007/s00284-024-04057-1

Ryan Yuki Huang, Chengye Zhang, Han Liang Lim

{"title":"Ribotyping Staphylococcus epidermidis Using Probabilistic Sequence Analysis and Levenshtein Distance Algorithm.","authors":"Ryan Yuki Huang, Chengye Zhang, Han Liang Lim","doi":"10.1007/s00284-024-04057-1","DOIUrl":"10.1007/s00284-024-04057-1","url":null,"abstract":"Staphylococcus epidermidis (S. epidermidis) live in different human locations and natural environments. For ribotyping S. epidermidis sub-species, 2507 PCR-amplified reads of 16S rRNA genes of S. epidermidis in a public dataset were used for probabilistic sequence analysis. A sequence probability logo (sequence pLogo) as a reference sequence of 16S rRNA genes of S. epidermidis was constructed. Through implementation of Levenshtein Distance algorithm, two 20-base pairs (bp) motifs, commonly present in 2507 PCR-amplified reads, were identified. The top 38 S. epidermidis isolates, which carried 16S rRNA nucleotide domains that were made of different sequences but have high similarity scores to two 20-bp motifs, were found from 11 human, 8 animal, 9 plant and 10 environmental samples, indicating that these two 20-bp motifs were broadly present in diverse S. epidermidis isolates. Thirty-one PCR-amplified reads of 16S rRNA genes, which were currently not in the dataset, were utilized to verify the feasibility of using two 20-bp motifs for ribotyping S. epidermidis sub-species. S. epidermidis S1, S3, but not S2, isolates on the human scalp carried a 20-bp sequence domain with high similarities to a 20-bp motif in the sequence pLogo. The phylogenetic tree showed that S. epidermidis S1, S2 and S3 were not from a single common ancestor. Two newly identified 20-bp motifs here, thus, provided reference nucleotide residues for ribotyping S. epidermidis.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 2","pages":"78"},"PeriodicalIF":2.3,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11723854/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142946364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeted Assay Engineering Enhances Bile Salt Hydrolase Activity in Heyndrickxia coagulans ATCC 7050 and Lactiplantibacillus plantarum ATCC 10012. 靶向检测工程提高了海因德里克夏凝血菌ATCC 7050和植物乳杆菌ATCC 10012的胆汁盐水解酶活性。

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-01-10 DOI: 10.1007/s00284-024-04028-6

Pratisha P Nair, Uday S Annapure

{"title":"Targeted Assay Engineering Enhances Bile Salt Hydrolase Activity in Heyndrickxia coagulans ATCC 7050 and Lactiplantibacillus plantarum ATCC 10012.","authors":"Pratisha P Nair, Uday S Annapure","doi":"10.1007/s00284-024-04028-6","DOIUrl":"10.1007/s00284-024-04028-6","url":null,"abstract":"The recent emergence of bile salt hydrolase (BSH) enzyme as a therapeutic target reflects its unbound potential in mitigating hypercholesterolemia, obesity, and gastrointestinal issues. However, to bolster its industrial application, optimization of BSH assay lays the cornerstone for enhancing sensitivity, specificity, and reproducibility. The current study delved into optimizing the BSH assay parameters utilizing response surface methodology (RSM) and one-factor-at-a-time (OFAT) method for two novel, natural BSH producers, Heyndrickxia coagulans ATCC 7050 and Lactiplantibacillus plantarum ATCC 10012. Factors such as pH, temperature, cell concentration, and substrate concentration were optimized using RSM and numerical optimization. The analysis of responses unveiled significant insights into the biochemical characteristics of BSH from both organisms. The optimal pH for BSH activity from H. coagulans ATCC 7050 and L. plantarum ATCC 10012 was determined to be 6.1 and 6.0, with their corresponding optimal temperatures being 60 °C and 40 °C, respectively. Subsequent to RSM, optimization of the remaining parameters such as buffer type, buffer molarity, cells-to-substrate ratio, etc., performed using the classical OFAT approach further enhanced BSH activity, with H. coagulans ATCC 7050 and L. plantarum ATCC 10012 exhibiting a 1.45 and 0.87-fold increase, respectively. Conventionally, even though BSH has been optimized using the OFAT approach, this is the first instance in which a hybrid model using RSM has been applied to achieve a greater performance. These findings offer valuable insights in augmenting the specificity, efficiency, and stability of BSH and broaching new avenues for industrial and therapeutic applications.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 2","pages":"77"},"PeriodicalIF":2.3,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142946371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation of New Strains of Lactic Acid Bacteria from the Vaginal Microbiome of Postmenopausal Women and their Probiotic Characteristics. 绝经后妇女阴道微生物群中乳酸菌新菌株的分离及其益生菌特性

IF 2.3 3区生物学

Current Microbiology Pub Date : 2025-01-09 DOI: 10.1007/s00284-024-04034-8

Indrajeet Barman, Hoonhee Seo, Sukyung Kim, Md Abdur Rahim, Youjin Yoon, Mohammed Solayman Hossain, Md Sarower Hossen Shuvo, Ho-Yeon Song

{"title":"Isolation of New Strains of Lactic Acid Bacteria from the Vaginal Microbiome of Postmenopausal Women and their Probiotic Characteristics.","authors":"Indrajeet Barman, Hoonhee Seo, Sukyung Kim, Md Abdur Rahim, Youjin Yoon, Mohammed Solayman Hossain, Md Sarower Hossen Shuvo, Ho-Yeon Song","doi":"10.1007/s00284-024-04034-8","DOIUrl":"10.1007/s00284-024-04034-8","url":null,"abstract":"Lactic acid bacteria (LAB), traditionally consumed as fermented foods, are now being applied to the medical field beyond health-functional food as probiotics. Therefore, it is necessary to continuously discover and evaluate new strains with suitable probiotic characteristics, mainly focusing on safety. In this study, we isolated eight new strains from postmenopausal vaginal fluid using culturomics approaches, an emerging area of interest. Data showed that most strains possessed significant cell surface hydrophobicity (≥ 76%), auto-aggregation capacity (17 to 61%), strong adhesion activity (8 to 34%), and excellent resistance to gastric acid, bile salt, and digestive enzyme, enhancing their survival in the gastrointestinal tract. Moreover, the strains exhibited functional characteristics, including substantial antibacterial activity with a minimal inhibitory concentration (MIC) ranging from 12.5 to 50%. They also harbored bacteriocins genes, produced short-chain fatty acids (acetate and propionate), exhibited significant phagocytic activity, possessed high antioxidative properties, rapidly depleted sodium nitrite, and exhibited proteolysis and β-glucosidase activity. In addition, heat-killed LAB strains significantly reduced the gene expressions of proinflammatory cytokines such as IL-β, IL-6, and iNOS in macrophages. Safety assessment revealed no cytotoxicity in macrophage cell lines. All strains tested negative for biogenic amine or H2O2 production, displayed no gelatinase or hemolytic activity, lacked virulence genes or detrimental enzymes, and displayed antibiotic susceptibility. In summary, these newly isolated strains demonstrate excellent probiotic functionality with a strong focus on safety, making them promising candidates for future drug development in the relevant fields.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 2","pages":"76"},"PeriodicalIF":2.3,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11717803/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142946403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0