Isolation and Characterization of A Novel Pbunavirus with Promising Antibiofilm Activity Against Pseudomonas Aeruginosa.

Abstract

Endolysin, encoded by bacteriophages, has emerged as a promising candidate against antibiotic-resistant bacteria due to its ability to target bacterial cell walls. In this study, we isolated a new bacteriophage vB_PaeC_sem1 from sewage. The genome of vB_PaeC_sem1 is 66,420 bp, a double-stranded linear DNA that comprises 92 open reading frames (ORFs). Sem1 showed broad stability across various temperatures, pH, and chemical solutions. Heterologous expression of Lys N in Escherichia coli was done (LysN1). The antibacterial effects of Lys N1 were shown against Pseudomonas aeruginosa and its biofilm disruption. Recombinant LysN1 and LysN effectively lysed P. aeruginosa as well as, destroying up to 80% and 67% of bacterial biofilm (respectively). No toxic effects on A549 cells were detected. These results suggest Sem1 as a potential alternative for treating Gram-negative bacterial infections.