Yea-Lin Moon, Kyung Hyun Kim, Ji-Yoon Shin, Jin-Sook Park
{"title":"从海绵中分离得到的碟皮鲁氏菌、空肠鲁氏菌和hyattella鲁氏菌。","authors":"Yea-Lin Moon, Kyung Hyun Kim, Ji-Yoon Shin, Jin-Sook Park","doi":"10.1007/s00284-025-04492-8","DOIUrl":null,"url":null,"abstract":"<p><p>Three Gram-stain-negative, strictly aerobic, cream-colored, catalase- and oxidase-positive bacterial strains, designated 2205SS24-7<sup>T</sup>, 2304DJ69-11<sup>T</sup>, and 2012CJ15-1<sup>T</sup>, were isolated from marine sponges collected in the Republic of Korea. The three novel strains grew optimally at 25-28 °C, pH 7.0, and 2-3% (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene and draft genome sequences revealed that the three novel strains clustered together with Ruegeria spongiae 2012CJ41-6<sup>T</sup>, forming a distinct clade within the genus Ruegeria. The 16S rRNA gene sequence similarities with R. spongiae were 97.6, 98.6, and 98.9%, respectively. Draft genome sequencing indicated genome sizes ranging from 5.2 to 6.1 Mb with DNA G+C contents between 59.0 and 60.5%. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values among the three novel strains ranged from 85.3-90.9% and 29.6-43.1%, respectively, indicating that each strain represents a separate species. Furthermore, ANI and dDDH values between the three novel strains and the type strains R. spongiae 2012CJ41-6<sup>T</sup>, R. mediterranea M17<sup>T</sup>, and R. litorea R-37<sup>T</sup> were ≤ 90.9% and ≤ 43.1%, respectively, both below the proposed thresholds for species delineation. The three novel strains possessed ubiquinone-10 as the major isoprenoid quinone and summed feature 8 (C<sub>18:1</sub> ω7c and/or C<sub>18:1</sub> ω6c) as the major fatty acid. The major polar lipids identified were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and phosphatidylcholine. Taken together, these results support the proposal that strain 2205SS24-7<sup>T</sup> (= KACC 23237<sup>T</sup> = LMG 33064<sup>T</sup>), 2304DJ69-11<sup>T</sup> (= KACC 23805<sup>T</sup> = MCCC 1K09534<sup>T</sup>), and 2012CJ15-1<sup>T</sup> (= KACC 23842<sup>T</sup> = MCCC 1K09526<sup>T</sup>) represent three novel species within the genus Ruegeria, for which the names Ruegeria discodermiae sp. nov., Ruegeria jejuensis sp. nov., and Ruegeria hyattellae sp. nov. are proposed, respectively.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 11","pages":"503"},"PeriodicalIF":2.6000,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Ruegeria discodermiae sp. nov., Ruegeria jejuensis sp. nov., and Ruegeria hyattellae sp. nov., Isolated from Marine Sponges.\",\"authors\":\"Yea-Lin Moon, Kyung Hyun Kim, Ji-Yoon Shin, Jin-Sook Park\",\"doi\":\"10.1007/s00284-025-04492-8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Three Gram-stain-negative, strictly aerobic, cream-colored, catalase- and oxidase-positive bacterial strains, designated 2205SS24-7<sup>T</sup>, 2304DJ69-11<sup>T</sup>, and 2012CJ15-1<sup>T</sup>, were isolated from marine sponges collected in the Republic of Korea. The three novel strains grew optimally at 25-28 °C, pH 7.0, and 2-3% (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene and draft genome sequences revealed that the three novel strains clustered together with Ruegeria spongiae 2012CJ41-6<sup>T</sup>, forming a distinct clade within the genus Ruegeria. The 16S rRNA gene sequence similarities with R. spongiae were 97.6, 98.6, and 98.9%, respectively. Draft genome sequencing indicated genome sizes ranging from 5.2 to 6.1 Mb with DNA G+C contents between 59.0 and 60.5%. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values among the three novel strains ranged from 85.3-90.9% and 29.6-43.1%, respectively, indicating that each strain represents a separate species. Furthermore, ANI and dDDH values between the three novel strains and the type strains R. spongiae 2012CJ41-6<sup>T</sup>, R. mediterranea M17<sup>T</sup>, and R. litorea R-37<sup>T</sup> were ≤ 90.9% and ≤ 43.1%, respectively, both below the proposed thresholds for species delineation. The three novel strains possessed ubiquinone-10 as the major isoprenoid quinone and summed feature 8 (C<sub>18:1</sub> ω7c and/or C<sub>18:1</sub> ω6c) as the major fatty acid. The major polar lipids identified were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and phosphatidylcholine. Taken together, these results support the proposal that strain 2205SS24-7<sup>T</sup> (= KACC 23237<sup>T</sup> = LMG 33064<sup>T</sup>), 2304DJ69-11<sup>T</sup> (= KACC 23805<sup>T</sup> = MCCC 1K09534<sup>T</sup>), and 2012CJ15-1<sup>T</sup> (= KACC 23842<sup>T</sup> = MCCC 1K09526<sup>T</sup>) represent three novel species within the genus Ruegeria, for which the names Ruegeria discodermiae sp. nov., Ruegeria jejuensis sp. nov., and Ruegeria hyattellae sp. nov. are proposed, respectively.</p>\",\"PeriodicalId\":11360,\"journal\":{\"name\":\"Current Microbiology\",\"volume\":\"82 11\",\"pages\":\"503\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2025-09-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s00284-025-04492-8\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s00284-025-04492-8","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Ruegeria discodermiae sp. nov., Ruegeria jejuensis sp. nov., and Ruegeria hyattellae sp. nov., Isolated from Marine Sponges.
Three Gram-stain-negative, strictly aerobic, cream-colored, catalase- and oxidase-positive bacterial strains, designated 2205SS24-7T, 2304DJ69-11T, and 2012CJ15-1T, were isolated from marine sponges collected in the Republic of Korea. The three novel strains grew optimally at 25-28 °C, pH 7.0, and 2-3% (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene and draft genome sequences revealed that the three novel strains clustered together with Ruegeria spongiae 2012CJ41-6T, forming a distinct clade within the genus Ruegeria. The 16S rRNA gene sequence similarities with R. spongiae were 97.6, 98.6, and 98.9%, respectively. Draft genome sequencing indicated genome sizes ranging from 5.2 to 6.1 Mb with DNA G+C contents between 59.0 and 60.5%. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values among the three novel strains ranged from 85.3-90.9% and 29.6-43.1%, respectively, indicating that each strain represents a separate species. Furthermore, ANI and dDDH values between the three novel strains and the type strains R. spongiae 2012CJ41-6T, R. mediterranea M17T, and R. litorea R-37T were ≤ 90.9% and ≤ 43.1%, respectively, both below the proposed thresholds for species delineation. The three novel strains possessed ubiquinone-10 as the major isoprenoid quinone and summed feature 8 (C18:1 ω7c and/or C18:1 ω6c) as the major fatty acid. The major polar lipids identified were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and phosphatidylcholine. Taken together, these results support the proposal that strain 2205SS24-7T (= KACC 23237T = LMG 33064T), 2304DJ69-11T (= KACC 23805T = MCCC 1K09534T), and 2012CJ15-1T (= KACC 23842T = MCCC 1K09526T) represent three novel species within the genus Ruegeria, for which the names Ruegeria discodermiae sp. nov., Ruegeria jejuensis sp. nov., and Ruegeria hyattellae sp. nov. are proposed, respectively.
期刊介绍：
Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment.
Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas:
physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.
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