Pseudoduganella rhizocola sp. nov., Isolated from Rhizospheric Soil.

IF 2.6 3区生物学 Q3 MICROBIOLOGY

Current Microbiology Pub Date : 2025-09-10 DOI:10.1007/s00284-025-04493-7

Shakiba S Chegini, Jaincy N Jayan, Yong Ho Kim, Sathiyaraj Srinivasan, Sang-Seob Lee

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引用次数: 0

Abstract

A novel bacterial strain, SM-13^T was isolated from the rhizospheric soil of Epipremnum aureum (Jade Pothos) sampled in Suwon, Republic of Korea. The isolate was Gram-stain-negative, aerobic, motile, rod-shaped, cream-coloured, oxidase- and catalase-positive. Strain SM-13^T grew at the range of 15-37 °C (optimum, 25 °C), at pH 6.0-9.0 (optimum, pH 7.0), and in the presence of 0-0.5% (w/v) NaCl (optimum, 0%). Phylogenetic analysis based on genome and 16S rRNA gene sequences indicated that strain SM-13^T was affiliated with the family Oxalobacteriaceae and most closely related to Pseudoduganella humi THG S6-8^T (97.8%), Pseudoduganella violacea CAVIO^T (97.7%), Pseudoduganella buxea A9^T (97.6%), Pseudoduganella dura 16^T (97.5%), Pseudoduganella umbonata LP01^T (97.5%), and Pseudoduganella lurida D5^T (97.4%). The major ubiquinone was Q-8. The major identified polar lipids were phosphatidylethanolamine (PE), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), amino lipids (AL), and unknown lipids (L1, L2). The major fatty acids of SM-13^T were summed feature 3 (C_16:1 ω7c/C_16:1 ω6c) (51.9%) and C_16: 0 (18.7%). The novel strain SM-13^T has a draft genome size of 5,401,088 bp (27 contigs), annotated with 4,880 protein-coding genes, 59 tRNA and 2 rRNA genes. The genomic DNA G + C (%) content was 64. Hence, the strain SM-13^T represent a novel species within the genus Pseudoduganella, for which the name Pseudoduganella rhizocola sp. nov. is proposed. The Type strain is SM-13^T (= KCTC 8734^T = TBRC 19389^T = KEMB 24220^T). The GenBank/EMBL/DDBJ accession number for the complete 16S rRNA gene and genome sequence of the type strain SM-13^T is PQ198503 and JBJQIK000000000.

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从根际土壤中分离的根瘤菌。

从韩国水原地区采得的金黄色葡萄（Epipremnum aureum, Jade Pothos）根际土壤中分离到一株新的细菌sm - 13t。革兰氏染色阴性，好氧，运动，棒状，米色，氧化酶和过氧化氢酶阳性。菌株sm - 13t在15-37℃（最适25℃）、pH 6.0-9.0（最适pH 7.0）和0-0.5% (w/v) NaCl（最适0%）条件下生长。基于基因组和16S rRNA基因序列的系统发育分析表明，菌株sm - 13t隶属于草藻科，与腐植菌THG S6-8 T（97.8%）、紫假杜氏甘菌CAVIOT（97.7%）、黄假杜氏甘菌A9T（97.6%）、硬假杜氏甘菌16t（97.5%）、黄假杜氏甘菌LP01T（97.5%）、lurida假杜氏甘菌D5T（97.4%）亲缘关系最为密切。主要的泛醌是Q-8。鉴定出的主要极性脂质有磷脂酰乙醇胺（PE）、磷脂酰甘油（PG）、二磷脂酰甘油（DPG）、氨基脂质（AL）和未知脂质（L1、L2）。sm - 13t的主要脂肪酸为特征3 (C16:1 ω7c/C16:1 ω6c)（51.9%）和C16: 0（18.7%）。新菌株sm - 13t的草图基因组大小为5,401,088 bp(27个contigs)，注释了4,880个蛋白质编码基因，59个tRNA和2个rRNA基因。基因组DNA G + C（%）含量为64。因此，菌株sm - 13t代表了Pseudoduganella属中的一个新种，因此建议将其命名为Pseudoduganella rhizocola sp. 11。型应变为sm - 13t （= KCTC 8734 T = TBRC 19389 T = KEMB 24220 T）。sm - 13t型菌株16S rRNA全基因和基因组序列的GenBank/EMBL/DDBJ登录号分别为PQ198503和JBJQIK000000000。

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来源期刊

Current Microbiology 生物-微生物学

CiteScore

4.80

自引率

3.80%

发文量

380

审稿时长

2.5 months

期刊介绍： Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.