Shakiba S Chegini, Jaincy N Jayan, Yong Ho Kim, Sathiyaraj Srinivasan, Sang-Seob Lee
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Phylogenetic analysis based on genome and 16S rRNA gene sequences indicated that strain SM-13<sup> T</sup> was affiliated with the family Oxalobacteriaceae and most closely related to Pseudoduganella humi THG S6-8<sup> T</sup> (97.8%), Pseudoduganella violacea CAVIO<sup>T</sup> (97.7%), Pseudoduganella buxea A9<sup>T</sup> (97.6%), Pseudoduganella dura 16<sup> T</sup> (97.5%), Pseudoduganella umbonata LP01<sup>T</sup> (97.5%), and Pseudoduganella lurida D5<sup>T</sup> (97.4%). The major ubiquinone was Q-8. The major identified polar lipids were phosphatidylethanolamine (PE), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), amino lipids (AL), and unknown lipids (L1, L2). The major fatty acids of SM-13<sup> T</sup> were summed feature 3 (C<sub>16:1</sub> ω7c/C<sub>16:1</sub> ω6c) (51.9%) and C<sub>16: 0</sub> (18.7%). The novel strain SM-13<sup> T</sup> has a draft genome size of 5,401,088 bp (27 contigs), annotated with 4,880 protein-coding genes, 59 tRNA and 2 rRNA genes. The genomic DNA G + C (%) content was 64. Hence, the strain SM-13<sup> T</sup> represent a novel species within the genus Pseudoduganella, for which the name Pseudoduganella rhizocola sp. nov. is proposed. The Type strain is SM-13<sup> T</sup> (= KCTC 8734<sup> T</sup> = TBRC 19389<sup> T</sup> = KEMB 24220<sup> T</sup>). The GenBank/EMBL/DDBJ accession number for the complete 16S rRNA gene and genome sequence of the type strain SM-13<sup> T</sup> is PQ198503 and JBJQIK000000000.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 10","pages":"498"},"PeriodicalIF":2.6000,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Pseudoduganella rhizocola sp. nov., Isolated from Rhizospheric Soil.\",\"authors\":\"Shakiba S Chegini, Jaincy N Jayan, Yong Ho Kim, Sathiyaraj Srinivasan, Sang-Seob Lee\",\"doi\":\"10.1007/s00284-025-04493-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A novel bacterial strain, SM-13<sup> T</sup> was isolated from the rhizospheric soil of Epipremnum aureum (Jade Pothos) sampled in Suwon, Republic of Korea. The isolate was Gram-stain-negative, aerobic, motile, rod-shaped, cream-coloured, oxidase- and catalase-positive. Strain SM-13<sup> T</sup> grew at the range of 15-37 °C (optimum, 25 °C), at pH 6.0-9.0 (optimum, pH 7.0), and in the presence of 0-0.5% (w/v) NaCl (optimum, 0%). Phylogenetic analysis based on genome and 16S rRNA gene sequences indicated that strain SM-13<sup> T</sup> was affiliated with the family Oxalobacteriaceae and most closely related to Pseudoduganella humi THG S6-8<sup> T</sup> (97.8%), Pseudoduganella violacea CAVIO<sup>T</sup> (97.7%), Pseudoduganella buxea A9<sup>T</sup> (97.6%), Pseudoduganella dura 16<sup> T</sup> (97.5%), Pseudoduganella umbonata LP01<sup>T</sup> (97.5%), and Pseudoduganella lurida D5<sup>T</sup> (97.4%). The major ubiquinone was Q-8. The major identified polar lipids were phosphatidylethanolamine (PE), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), amino lipids (AL), and unknown lipids (L1, L2). The major fatty acids of SM-13<sup> T</sup> were summed feature 3 (C<sub>16:1</sub> ω7c/C<sub>16:1</sub> ω6c) (51.9%) and C<sub>16: 0</sub> (18.7%). The novel strain SM-13<sup> T</sup> has a draft genome size of 5,401,088 bp (27 contigs), annotated with 4,880 protein-coding genes, 59 tRNA and 2 rRNA genes. The genomic DNA G + C (%) content was 64. Hence, the strain SM-13<sup> T</sup> represent a novel species within the genus Pseudoduganella, for which the name Pseudoduganella rhizocola sp. nov. is proposed. The Type strain is SM-13<sup> T</sup> (= KCTC 8734<sup> T</sup> = TBRC 19389<sup> T</sup> = KEMB 24220<sup> T</sup>). 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Pseudoduganella rhizocola sp. nov., Isolated from Rhizospheric Soil.
A novel bacterial strain, SM-13 T was isolated from the rhizospheric soil of Epipremnum aureum (Jade Pothos) sampled in Suwon, Republic of Korea. The isolate was Gram-stain-negative, aerobic, motile, rod-shaped, cream-coloured, oxidase- and catalase-positive. Strain SM-13 T grew at the range of 15-37 °C (optimum, 25 °C), at pH 6.0-9.0 (optimum, pH 7.0), and in the presence of 0-0.5% (w/v) NaCl (optimum, 0%). Phylogenetic analysis based on genome and 16S rRNA gene sequences indicated that strain SM-13 T was affiliated with the family Oxalobacteriaceae and most closely related to Pseudoduganella humi THG S6-8 T (97.8%), Pseudoduganella violacea CAVIOT (97.7%), Pseudoduganella buxea A9T (97.6%), Pseudoduganella dura 16 T (97.5%), Pseudoduganella umbonata LP01T (97.5%), and Pseudoduganella lurida D5T (97.4%). The major ubiquinone was Q-8. The major identified polar lipids were phosphatidylethanolamine (PE), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), amino lipids (AL), and unknown lipids (L1, L2). The major fatty acids of SM-13 T were summed feature 3 (C16:1 ω7c/C16:1 ω6c) (51.9%) and C16: 0 (18.7%). The novel strain SM-13 T has a draft genome size of 5,401,088 bp (27 contigs), annotated with 4,880 protein-coding genes, 59 tRNA and 2 rRNA genes. The genomic DNA G + C (%) content was 64. Hence, the strain SM-13 T represent a novel species within the genus Pseudoduganella, for which the name Pseudoduganella rhizocola sp. nov. is proposed. The Type strain is SM-13 T (= KCTC 8734 T = TBRC 19389 T = KEMB 24220 T). The GenBank/EMBL/DDBJ accession number for the complete 16S rRNA gene and genome sequence of the type strain SM-13 T is PQ198503 and JBJQIK000000000.
期刊介绍：
Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment.
Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas:
physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.
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