{"title":"Biotic Flow of Carbon and Phosphorus in the Lake-Catchment System.","authors":"V V Boulion","doi":"10.1134/S0012496625600861","DOIUrl":"10.1134/S0012496625600861","url":null,"abstract":"<p><p>To assess the flow of organic carbon in a lake ecosystem surrounded by terrestrial vegetation, a mass-balance model was developed based on a concept that interprets vegetation production as a function of heat and moisture. The concept was complemented by a description of the rates of mineralization of vegetation products and removal of released nutrients into the aquatic environment. Biogenic elements and solar radiation initiate the synthesis and biotic flow of organic matter in the aquatic community. The model is designed to predict the biomass and annual production of terrestrial vegetation and key groups of aquatic organisms, including fish, in lake-type systems in the Northern Hemisphere.</p>","PeriodicalId":11351,"journal":{"name":"Doklady Biological Sciences","volume":" ","pages":"102-109"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147812470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A V Lavrov, A V Lopatin, D R Khantemirov, D O Gimranov
{"title":"Cranial Findings of Pachycrocuta brevirostris (Hyaenidae, Carnivora) from the Taurida Cave (Crimea, Early Pleistocene).","authors":"A V Lavrov, A V Lopatin, D R Khantemirov, D O Gimranov","doi":"10.1134/S001249662560085X","DOIUrl":"10.1134/S001249662560085X","url":null,"abstract":"<p><p>Cranial materials of the large hyena Pachycrocuta brevirostris (Gervais, 1850) from the Early Pleistocene (~1.8-1.6 Ma) locality in the Taurida cave (Crimea) belong to individuals of different individual ages, from juvenile to senile. The relative facial length of the \"short-faced hyena\" P. brevirostris has been shown to be greater than that of the recent spotted hyena Crocuta сrocuta (Erxleben, 1777). As P. brevirostris matured, the facial and frontal regions increased significantly in height, and the dorsal profile line of the skull elevated noticeably. New material on P. brevirostris is of great importance for studying the geographical variability and evolution of this widespread species of carnivorous mammals of the Early Pleistocene.</p>","PeriodicalId":11351,"journal":{"name":"Doklady Biological Sciences","volume":" ","pages":"95-101"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147728753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Mellivora indosinica sp. nov. (Mustelidae, Carnivora): a Honey Badger from the Pleistocene of Vietnam.","authors":"A V Lopatin, D O Gimranov","doi":"10.1134/S0012496626600016","DOIUrl":"10.1134/S0012496626600016","url":null,"abstract":"<p><p>A new honey badger, Mellivora indosinica sp. nov., is described on the basis of an isolated lower carnassial tooth from the Middle Pleistocene deposits of the Tham Hai cave in Northeast Vietnam (Lang Son Province). Judging by tooth measurements, the new species is larger than all known Mellivorini, including the medium-sized Recent Mellivora capensis, but much smaller than the Neogene giant Eomellivorini. This is the only representative of Mellivorinae in Southeast Asia and evidence for the presence of honey badgers in the Pleistocene Stegodon-Ailuropoda fauna.</p>","PeriodicalId":11351,"journal":{"name":"Doklady Biological Sciences","volume":" ","pages":"81-87"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147728687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A V Fialko, Yu A Yugay, E A Vasyutkina, V P Grigorchuk, Yu N Shkryl, V P Bulgakov
{"title":"Formation of Adaptive Defense to Low Temperatures in Arabidopsis thaliana.","authors":"A V Fialko, Yu A Yugay, E A Vasyutkina, V P Grigorchuk, Yu N Shkryl, V P Bulgakov","doi":"10.1134/S0012496625600848","DOIUrl":"10.1134/S0012496625600848","url":null,"abstract":"<p><p>The effects of repeated cold stress on physiological, transcriptional, and metabolic processes was studied in Arabidopsis thaliana. An experiment included stages of initial chilling, acclimation, and repeated cold treatment. Repeated cold stress increased electrolyte leakage and decreased the chlorophyll a content, indicating a disruption of the membrane integrity and a restructuring of the photosynthetic apparatus. Expression analysis showed upregulation of the DREB1A, MYC2, COR15A, and COR15B genes, which are associated with the cold response and stress memory formation. Transcription of the flowering regulators FLC, HDA6, and HD2C was altered at the same time to balance adaptation and development. HPLC-MS revealed a significant accumulation of flavonoids, primarily kaempferol and quercetin derivatives, suggesting activation of antioxidant defenses. Taken together, the findings confirmed that repeated cold treatment activates a complex plant adaptation system based on the integration of physiological, transcriptional, and metabolic mechanisms. The formation of stress memory allows A. thaliana to maintain resistance and functional stability during repeated low-temperature exposures.</p>","PeriodicalId":11351,"journal":{"name":"Doklady Biological Sciences","volume":" ","pages":"88-94"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147728709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Convergence and Parallelism of Phenotypic and Molecular Genetic Variability in Some Species.","authors":"G Yu Kosovsky, T T Glazko","doi":"10.1134/S0012496625600666","DOIUrl":"10.1134/S0012496625600666","url":null,"abstract":"<p><p>In-depth knowledge of genome organization is necessary for developing genome editing methods. In mammals, retrotransposons, in which short and long interspersed repeats (SINEs and LINEs) predominate, account for about half of the genome and are involved in regulating gene expression. Retrotransposons are classified into ancient and younger, which gradually displace ancient variants. Species- and gene-related features of the displacement were observed by analyzing retrotransposons in the loci of the common gene editing targets myostatin (MSTN) and leptin receptor (LEPR) in humans, rabbits, cattle, and house mice. A higher preservation of ancient variants was detected in MSTN-flanking genes in rabbits and LEPR-flanking genes in humans. Functional convergence of genomic elements affecting intragenomic architectonics is discussed. Functional convergence of transposons is assumed to perform the same supporting function in genome architecture as stromal elements do in tissues.</p>","PeriodicalId":11351,"journal":{"name":"Doklady Biological Sciences","volume":" ","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147580909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N A Berezina, L E Sigareva, V S Vishnyakov, N A Timofeeva, A N Sharov, A V Krylov
{"title":"Analysis of Diatom Algae and Phytopigments in Bottom Sediments to Understand Function of Subarctic Glacial Lake.","authors":"N A Berezina, L E Sigareva, V S Vishnyakov, N A Timofeeva, A N Sharov, A V Krylov","doi":"10.1134/S0012496625600551","DOIUrl":"10.1134/S0012496625600551","url":null,"abstract":"<p><p>Lakes of the White Sea coast have altered owing to postglacial land uplift, changing from marine lagoon to freshwater conditions. The Lake Krivoe (deep, transparent, and stratified) at the Cape Kartesh (northern Karelia) formed via isolation from the sea and is made up of two basins filled with organic-rich sediments. As for the trophic status, the pelagic zone of the lake has remained oligotrophic since the 1960s. Diatoms and phytopigments were studied in a sediment core from a deep part of the lake. A high concentration of valves of benthic and periphytonic diatom species was observed in the core, indicating a significant contribution of these producers to the lake productivity. The lake bottom sediments were classified as highly productive (hypertrophic) based on their phytopigment concentration. Thus, benthic producers can play a key role in the ecosystems of arctic lakes, and the idea is important to consider when monitoring.</p>","PeriodicalId":11351,"journal":{"name":"Doklady Biological Sciences","volume":" ","pages":"8-12"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147580860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Phenolic Compounds and Elemental Composition of Rhodiola rosea (Crassulaceae) from Altai Mountains.","authors":"L N Zibareva, A S Prokopyev, E S Rabtsevich","doi":"10.1134/S0012496625700152","DOIUrl":"10.1134/S0012496625700152","url":null,"abstract":"<p><p>Phenolic compounds in the underground parts (rhizomes with adventitious roots) were studied comparatively in Rhodiola rosea L. (Crassulaceae) plants collected from natural cenopopulations of the Altai Mountains and cultivated in the Siberian Botanical Garden (Tomsk). Gallic acid, salidroside, and rosavin were identified in the samples. The salidroside content in wild R. rosea samples varied from 0.02 to 0.48% of the absolute dry weight; the rosavin content was 0.77-1.57%. The gallic acid content was approximately the same, 0.1%, in all samples. Cultivated R. rosea plants had a higher salidroside content in the underground organs as compared with plants from natural populations with a similar rosavin content. A rich and diverse elemental composition of the underground parts was observed in R. rosea plants growing in the Altai Mountains. In addition to common macro and trace elements, such as Mg, K, Fe, Mn, Rb, Cr, Sr, Zn, Ba, Mo, and Co, rare elements were detected, including Ti, Ga, Nb, La, Ho, Gd, etc. Based on the contents of chemical elements in the underground organs and soil, the R. rosea samples of different origins were divided into three groups. One group was characterized by a low element content in plants and a high content in soil. A second group was distinguished by a high content of elements in the underground plant parts and a low content in soil. A third group showed a high element content in both the underground plant parts and soil.</p>","PeriodicalId":11351,"journal":{"name":"Doklady Biological Sciences","volume":" ","pages":"58-68"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147580877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N V Selivanova, M A Pustynnaya, M A Gusakova, K G Bogolitsyn
{"title":"Biologically Active Compounds from Bark of Juniperus communis (Cupressaceae).","authors":"N V Selivanova, M A Pustynnaya, M A Gusakova, K G Bogolitsyn","doi":"10.1134/S0012496625700164","DOIUrl":"10.1134/S0012496625700164","url":null,"abstract":"<p><p>The component composition of the bark was studied in Juniperus communis L. (Cupressaceae). The juniper bark was shown to contain 3.5 times more extractives than wood. Individual component compositions of ethanol and petroleum extracts of J. communis bark was determined by gas chromatography-mass spectrometry. The ethanol and petroleum bark extracts were found to differ in component composition. Monosaccharides, disaccharides, and resin acids were major constituents of the J. communis ethanol extract, while resin acids, fatty acids, and sterols were major constituents of the petroleum extract. High antiradical activity was observed in the ethanol extract of J. communis bark. Inhibition of the ABTS radical activity by the ethanol bark extract reached 86-87%. Based on the findings, the bark of J. communis from the subarctic region was concluded to provide a source of valuable bioactive substances.</p>","PeriodicalId":11351,"journal":{"name":"Doklady Biological Sciences","volume":" ","pages":"69-74"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147580871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M R Sorokina, Yu A Yugay, A I Degtyarenko, A A Sadykov, Yu N Shkryl, V P Bulgakov
{"title":"Comprehensive Optimization of Agroinfiltration in Transient Expression System in Nicotiana tabacum with RUBY Visible Reporter.","authors":"M R Sorokina, Yu A Yugay, A I Degtyarenko, A A Sadykov, Yu N Shkryl, V P Bulgakov","doi":"10.1134/S0012496625600721","DOIUrl":"10.1134/S0012496625600721","url":null,"abstract":"<p><p>Agrobacterium-mediated transient expression is one of the most popular tools in functional genomics and plant biotechnology. However, its efficiency and reproducibility depend on multiple factors. Agroinfiltration conditions were optimized comprehensively to enhance the expression level of betalain biosynthetic genes in the model plant Nicotiana tabacum with the use of the RUBY visual reporter system. The effects of bacterial suspension concentration, infiltration buffer composition, and delivery method were evaluated. The highest expression efficiency was achieved with a suspension of OD<sub>600</sub> = 1.0; a buffer containing acetosyringone (50 μM), 2-(N-morpholino)ethanesulfonic acid (MES) (10 mM), sucrose (0.5%), and magnesium chloride (10 mM); and delivery via preliminary leaf micropuncture with a needle roller and subsequent vacuum infiltration. The resulting protocol significantly improves the efficiency of transient expression and can be employed in gene functional analysis, metabolic engineering, and production of biopharmaceutical compounds in plants.</p>","PeriodicalId":11351,"journal":{"name":"Doklady Biological Sciences","volume":" ","pages":"13-19"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147580902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L A Ilchuk, M V Kubekina, Yu D Okulova, E N Korshunov, M A Filatov, A V Bruter, Yu Yu Silaeva
{"title":"Generation of Stra8-Cre/ERT2 Mouse Activator Strain for Inducible Cre-lox Recombination in Spermatocytes.","authors":"L A Ilchuk, M V Kubekina, Yu D Okulova, E N Korshunov, M A Filatov, A V Bruter, Yu Yu Silaeva","doi":"10.1134/S0012496625600770","DOIUrl":"10.1134/S0012496625600770","url":null,"abstract":"<p><p>Reverse genetics often requires systems that allow intended induction of toxic genes or inactivation of vital genes at a chosen moment and/or in a chosen tissue. Cre-lox recombination is a common tool of genetic engineering and is used to conditionally knock out a gene, in which an exon is preliminarily flanked with loxP sites, or to induce expression of a gene, in which a loxP-flanked STOP cassette is preliminarily inserted between the promoter and the open reading frame. A mouse strain, Stra8-Cre/ER<sup>T2</sup>, was constructed to allow tamoxifen induction of Cre-lox recombination in spermatocytes. Construction was performed using a 400-bp promoter of the Stra8 gene, whose protein product STRA8 initiates the transition from mitosis to meiosis in response to a growing concentration of retinoic acid in the cell. The strain will be further used to study the regulatory mechanisms of meiosis.</p>","PeriodicalId":11351,"journal":{"name":"Doklady Biological Sciences","volume":" ","pages":"20-23"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147580885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}