Doklady Biological Sciences最新文献_第4页

Calcium Signaling Modulator 4-MPTC Reduces Proliferative and Migration Activities of MDA-MB-468 Breast Cancer Cells. 钙信号调节剂4-MPTC降低MDA-MB-468乳腺癌细胞的增殖和迁移活性

Doklady Biological Sciences Pub Date : 2026-02-01 Epub Date: 2026-03-30 DOI: 10.1134/S0012496625600836

A Yu Skopin, K O Gusev, D A Grekhnev, L N Glushankova, V G Kartsev, E V Kaznacheyeva

引用次数: 0

MYCN Gene Is Activated by High-Level Expression of Stress-Inducible Isoform of Oct-1 Transcription Factor in Human Neuroblastoma Cells. 应激诱导的Oct-1转录因子异构体在人神经母细胞瘤细胞中高水平表达激活MYCN基因

Doklady Biological Sciences Pub Date : 2026-02-01 Epub Date: 2026-03-30 DOI: 10.1134/S0012496625600691

E V Pankratova, T N Portseva, B M Lyanova, S G Georgieva, A G Stepchenko

{"title":"MYCN Gene Is Activated by High-Level Expression of Stress-Inducible Isoform of Oct-1 Transcription Factor in Human Neuroblastoma Cells.","authors":"E V Pankratova, T N Portseva, B M Lyanova, S G Georgieva, A G Stepchenko","doi":"10.1134/S0012496625600691","DOIUrl":"10.1134/S0012496625600691","url":null,"abstract":"MYCN is expressed in the developing nervous system and is vital for proliferation, migration, and homeostasis of differentiating neuronal cells. Its expression decreases during neuronal differentiation. A high level of MYCN expression in neuroblastoma cells is a negative prognostic marker, and MYCN inhibition provides an efficient means to prevent tumor growth. Understanding MYCN regulation at the transcriptional level can help identify the regulatory pathways that may be targeted to reduce MYCN expression. The regulatory region of the MYCN gene was shown to contain binding sites for the transcription factor Oct-1 (POU2F1). Several Oct-1 isoforms present in IMR32 neuroblastoma cells were tested for effect on MICN expression, and only the primate-specific, stress-inducible isoform Oct-1Z was found to regulate MYCN in IMR32 cells during neuronal differentiation. High-level expression of Oct-1Z increased expression of MYCN and its target gene NEUROD1. Oct-1Z was thus identified as an activator of MYCN expression and a potential target for neuroblastoma therapy in patients with high levels of MYCN.","PeriodicalId":11351,"journal":{"name":"Doklady Biological Sciences","volume":" ","pages":"75-79"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147580864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Component Composition of Aerial Parts in Centaurea Species (Asteraceae) from Siberia and Central Asia. 标题西伯利亚和中亚半人马属植物地上部成分的研究。

Doklady Biological Sciences Pub Date : 2026-02-01 Epub Date: 2026-03-30 DOI: 10.1134/S0012496625700140

E A Kasterova, A S Revushkin, A L Ebel

{"title":"Component Composition of Aerial Parts in Centaurea Species (Asteraceae) from Siberia and Central Asia.","authors":"E A Kasterova, A S Revushkin, A L Ebel","doi":"10.1134/S0012496625700140","DOIUrl":"10.1134/S0012496625700140","url":null,"abstract":"The component composition of aerial parts was studied in six species of the genus Centaurea L. s.l. (C. modesti Fed., C. phyllopoda Iljin, C. gontscharovii Iljin, C. ruthenica Lam., C. scabiosa L., and C. sibirica L.) from Siberia and Central Asia. The species C. modesti, C. phyllopoda, and C. gontscharovii were for the first time studied in this respect. Using high-performance liquid chromatography (HPLC), 24 phenolic compounds were identified, of which 15 were flavonoids and 9 were phenolic acids. Ethyl gallate was identified in all of the species. Avicularin, baicalin, and apigenin were characteristic of all but one Central Asian species, C. gontscharovii. Gallic acid was identified in all species except C. sibirica. Chlorogenic acid was characteristic of all species except C. modestii. Syringin, salipurposide, avicularin, baicalin, and apigenin were found in aerial part extracts of C. ruthenica from Kazakhstan and C. phyllopoda. Dihydromyricitin was identified only in C. ruthenica from Kazachstan. Eriodictyol and ellagic acid were identified only in C. scabiosa; fumaric and salicylic acids, only in C. phyllopoda. The content of phenolic compounds was 0.6-6.7% of dry weight in the species under study.","PeriodicalId":11351,"journal":{"name":"Doklady Biological Sciences","volume":" ","pages":"41-46"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147580842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pyrrolizidine Alkaloids and Toxic Microelements in Above-Ground Parts of Nonea rossica (Boraginaceae). 蔷薇地上部吡咯里西啶类生物碱及有毒微量元素研究。

Doklady Biological Sciences Pub Date : 2026-02-01 Epub Date: 2026-03-30 DOI: 10.1134/S0012496625700139

V V Velichko, D S Kruglov, D N Olennikov, K I Ershov, M E Kartashova

引用次数: 0

Comparison of Fatty Acid Composition of Lipids in Seeds of Several Salvia Species (Lamiaceae). 几种鼠尾草科鼠尾草种子脂类脂肪酸组成的比较。

Doklady Biological Sciences Pub Date : 2026-02-01 Epub Date: 2026-03-30 DOI: 10.1134/S0012496625700115

E V Feskova, V N Leontiev, A A Kisliakova, I N Tychina, T V Gil, B Yu Anoshenko, V V Titok

引用次数: 0

Role of Biologically Active Compounds of Plants in Improving Their Self-Resistance to Adverse Abiotic Factors. 植物生物活性化合物在提高植物对不利非生物因子的自我抗性中的作用。

Doklady Biological Sciences Pub Date : 2026-02-01 Epub Date: 2026-03-30 DOI: 10.1134/S0012496625700127

E S Vasfilova

{"title":"Role of Biologically Active Compounds of Plants in Improving Their Self-Resistance to Adverse Abiotic Factors.","authors":"E S Vasfilova","doi":"10.1134/S0012496625700127","DOIUrl":"10.1134/S0012496625700127","url":null,"abstract":"Various abiotic stressors universally affect the metabolic processes in plants, significantly limiting their growth and reducing their productivity. The stress factors include extreme temperatures, drought, salinity, UV radiation, and heavy metals. Plants, as organisms deprived of mobility, have developed complex and well-organized regulatory mechanisms to adapt to and resist abiotic stress conditions. In particular, the mechanisms utilize various biologically active compounds as tools to increase plant resistance to adverse environmental influences. The set includes primary metabolites (oligosaccharides, polysaccharides, their derivatives, polyols, amino acids, etc.) and secondary metabolites (terpenoids and phenolic compounds, such as flavonoids and phenolic acids). The compounds possess high antioxidant properties, suppress the accumulation and remove reactive oxygen species (ROS), and protect biological macromolecules (proteins, lipids, and nucleic acids) and cell structures from oxidative damage. The compounds play an active role in osmotic adaptation; chelate heavy metal ions to reduce their toxicity; and can function as primary signaling molecules to regulate the signals that control expression of many genes and enzymes involved in metabolic processes and associated with stress resistance. The biologically active compounds are important for adaptation and ensure the survival, stability, and competitiveness of plants in response to adverse environmental impacts.","PeriodicalId":11351,"journal":{"name":"Doklady Biological Sciences","volume":" ","pages":"24-35"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147580932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of Hox Genes in Early Development of Irregular Sea Urchin Scaphechinus mirabilis (Echinodermata, Echinoidea). Hox基因在不规则海胆scapechinus mirabilis早期发育中的表达

Doklady Biological Sciences Pub Date : 2025-12-01 Epub Date: 2025-12-02 DOI: 10.1134/S0012496625600526

O V Ezhova, N V Ageenko, K V Kiselev, V V Malakhov

{"title":"Expression of Hox Genes in Early Development of Irregular Sea Urchin Scaphechinus mirabilis (Echinodermata, Echinoidea).","authors":"O V Ezhova, N V Ageenko, K V Kiselev, V V Malakhov","doi":"10.1134/S0012496625600526","DOIUrl":"10.1134/S0012496625600526","url":null,"abstract":"Current data indicate that the Hox gene expression pattern differs between regular and irregular sea urchins. Expression of most genes of the Hox cluster is not activated until the pluteus forms, and only Hox7, 11/13b, and 11/13c are expressed at the blastula stage in two Regularia species examined previously, Strongylocentrotus purpuratus and St. intermedius. In contrast, in Peronella japonica, the only species studied in Irregularia, Hox7, 9/10, 11/13b, and 11/13c are expressed at the blastula and gastrula stages; Hox1, 7, 8, 9/10, 11/13a, and 11/13b are expressed at the prism stage; and most genes of the Hox cluster are activated at the pluteus stage. The irregular sea urchin Scaphechinus mirabilis was examined. Almost all of its Hox genes were silent at early stages. SmHox11/13b was an exception and was expressed at the gastrula stage. Most Hox genes were activated only at the pluteus stage. Thus, the expression pattern of the Hox cluster in Sc. mirabilis is similar to that of the regular sea urchins St. purpuratus and St. intermedius and differs from that of the other irregular sea urchin P. japonica.","PeriodicalId":11351,"journal":{"name":"Doklady Biological Sciences","volume":" ","pages":"341-345"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12795854/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145653990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microplastics in the Gastrointestinal Tracts of Striped Field Mice (Apodemus agraruis Pallas, 1771) from Moscow Parks. 莫斯科公园条纹田鼠胃肠道中的微塑料。

Doklady Biological Sciences Pub Date : 2025-12-01 Epub Date: 2026-01-12 DOI: 10.1134/S0012496625600599

T N Karmanova, V V Belenkova, N Yu Feoktistova, S I Meschersky, A V Surov

引用次数: 0

Doklady Biological Sciences Pub Date : 2025-12-01 Epub Date: 2026-01-12 DOI: 10.1134/S0012496625600472

M A Rodin, M V Kuznetsova, M Yu Krupnova, A E Kuritsyn, S A Murzina, N N Nemova

引用次数: 0

Generation of Long-Lived CHO Cells Suitable for Production of Afucosylated Antibodies and Fc-Fusion Proteins. 适合生产A聚焦抗体和fc融合蛋白的长寿命CHO细胞的产生。

Doklady Biological Sciences Pub Date : 2025-12-01 Epub Date: 2025-12-02 DOI: 10.1134/S0012496625600587

D E Kolesov, N A Orlova, I I Vorobiev

{"title":"Generation of Long-Lived CHO Cells Suitable for Production of Afucosylated Antibodies and Fc-Fusion Proteins.","authors":"D E Kolesov, N A Orlova, I I Vorobiev","doi":"10.1134/S0012496625600587","DOIUrl":"10.1134/S0012496625600587","url":null,"abstract":"Using genome editing, we created a homozygous α-(1,6)-fucosyltransferase (FUT8-/-) knockout in apoptosis-resistant CHO 4BGD cells, yielding the new 4BGD-F cell line. Combining CRISPR/Cas9 with paired gRNAs and non-specific puromycin selection yielded a cell population with an exceptionally high FUT8 knockout frequency, obviating the need for metabolic enrichment with lentil lectin (Lens culinaris agglutinin, LCA). Despite impaired clonogenicity of the knockout cells, we successfully isolated multiple clonal cell lines harboring extensive biallelic FUT8 deletions. Isolated clones with biallelic deletions retained key parental line characteristics: viability >90% in 17-day fed-batch cultures at high densities (>15 × 106 cells/mL), and rapid selectability using both dihydrofolate reductase and glutamine synthetase systems. Mass spectrometric analysis of the test protein GLP1-Fc secreted by 4BGD-F cells confirmed the absence of N-glycan fucosylation. The CHO 4BGD-F cell line provides a valuable platform for producing afucosylated antibodies with enhanced antibody-dependent cellular cytotoxicity.","PeriodicalId":11351,"journal":{"name":"Doklady Biological Sciences","volume":" ","pages":"359-367"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145654001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0