Doklady Biological Sciences最新文献

Comparative Analysis of the Amino Acid Spectrum of Blood Plasma in Adults and Juveniles of the Pond Bat (Myotis dasycneme Boie, 1825) from Natural Populations of the Urals. 乌拉尔自然种群池蝠（Myotis dasycneme Boie, 1825）成虫与幼虫血浆氨基酸谱的比较分析。

Doklady Biological Sciences Pub Date : 2026-08-03 DOI: 10.1134/S0012496626600272

L A Kovalchuk, L V Chernaya, N V Mikshevich, V N Bolshakov

引用次数: 0

Optimization of Exosome Isolation from Vitis vinifera Cell Cultures. 葡萄细胞外泌体分离的优化。

Doklady Biological Sciences Pub Date : 2026-08-03 DOI: 10.1134/S0012496626600338

M R Sorokina, A V Fialko, Yu A Yugay, O V Grishchenko, Yu N Shkryl, V P Bulgakov

{"title":"Optimization of Exosome Isolation from Vitis vinifera Cell Cultures.","authors":"M R Sorokina, A V Fialko, Yu A Yugay, O V Grishchenko, Yu N Shkryl, V P Bulgakov","doi":"10.1134/S0012496626600338","DOIUrl":"https://doi.org/10.1134/S0012496626600338","url":null,"abstract":"Plant extracellular vesicles (exosomes) are considered promising nanostructures for biomedical and biotechnological applications; however, methods for their isolation from plant cell cultures remain insufficiently optimized. The aim of this study was to evaluate the efficiency of exosome isolation from callus and suspension cultures of Vitis vinifera and to investigate the effects of culture age and extraction method on the yield of extracellular vesicles. It was demonstrated that suspension culture provides a higher exosome yield compared to callus tissue. Isolated particles were characterized by a size of 90-120 nm and a negative zeta potential, which corresponded to typical properties of plant extracellular vesicles. The maximum exosome yield was observed with a two-week-old suspension culture, which corresponded to the phase of intense cell growth. Comparison of extraction methods revealed that mechanical homogenization using a blender was the most efficient approach for exosome isolation, providing a high particle yield without significant deterioration in sample purity. The results demonstrate the potential of V. vinifera suspension cultures as a scalable platform for the production of plant-derived exosomes and may be useful for the development of biotechnological systems for the isolation of plant extracellular vesicles.","PeriodicalId":11351,"journal":{"name":"Doklady Biological Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148668588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Taxonomic Delineation of Fusarium weifangense within the F. incarnatum-equiseti Species Complex. 魏凡镰刀菌在猪镰孢菌群中的分类划分。

Doklady Biological Sciences Pub Date : 2026-08-03 DOI: 10.1134/S0012496626600193

T Yu Gagkaeva, A S Orina, O P Gavrilova

{"title":"Taxonomic Delineation of Fusarium weifangense within the F. incarnatum-equiseti Species Complex.","authors":"T Yu Gagkaeva, A S Orina, O P Gavrilova","doi":"10.1134/S0012496626600193","DOIUrl":"https://doi.org/10.1134/S0012496626600193","url":null,"abstract":"Modern delineation of the Fusarium species using genetic data faces challenges no less complex than those previously encountered, when species descriptions were based exclusively on morphological characteristics. In present study, the taxonomic status of five fungal strains belonging to the F. incarnatum-equiseti species complex, which is a highly heterogeneous group of Fusarium, was clarified. The strains were isolated from wild and cultivated grasses and maintained in the fungal collections for 13-18 years under the epithet F. semitectum (syn. F. incarnatum). More precise identification of these strains as F. weifangense was achieved by combining phylogenetic analysis of fragments of the translation elongation factor 1α gene, the gene encoding RNA polymerase subunit II analyses, and calmodulin gene with morphological description and pathogenicity evaluation. The results of a comparative analysis of the phenotypic characteristics of the strains with those previously reported for this species, which are largely inconsistent. That differences in morphological description may be due to internal polymorphism of taxon, as well as differences in methodological approaches used by different research groups. The analyzed strains caused weak necrosis of wheat leaves inoculated under laboratory conditions, which indicates that F. weifangense is a non-pathogenic fungus. However, the previously demonstrated ability of the F. weifangense strains to produce zearalenone and diacetoxyscirpenol makes it necessary to clarify area and adaptive properties of this fungus.","PeriodicalId":11351,"journal":{"name":"Doklady Biological Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148668596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Larger Owls (Aves: Strigidae) from the Lower Pleistocene of Crimea. 克里米亚下更新世较大的猫头鹰（鸟：鸮科）。

Doklady Biological Sciences Pub Date : 2026-08-03 DOI: 10.1134/S0012496626600284

N V Zelenkov

引用次数: 0

Continuous Light Exposure as Factor in Pro-Senescent Liver Remodeling: Key Role of Melatonin Deficiency. 持续光照在促衰老肝脏重塑中的作用：褪黑素缺乏的关键作用。

Doklady Biological Sciences Pub Date : 2026-08-03 DOI: 10.1134/S0012496626600314

D A Areshidze, M A Kozlova, A I Anurkina, A A Moskalev

引用次数: 0

Lipid Class Composition in the Freshwater Pearl Mussel Margaritifera margaritifera from Rivers of the White and Barents Sea Basins. 白海和巴伦支盆地河流淡水珍珠贻贝的脂类组成。

Doklady Biological Sciences Pub Date : 2026-08-03 DOI: 10.1134/S001249662660034X

S N Khurtina, V P Voronin, E P Ieshko, N N Fokina, D A Efremov, S A Murzina

{"title":"Lipid Class Composition in the Freshwater Pearl Mussel Margaritifera margaritifera from Rivers of the White and Barents Sea Basins.","authors":"S N Khurtina, V P Voronin, E P Ieshko, N N Fokina, D A Efremov, S A Murzina","doi":"10.1134/S001249662660034X","DOIUrl":"https://doi.org/10.1134/S001249662660034X","url":null,"abstract":"The contents of total lipids (TL) and individual lipid classes were studied in the mantle, foot, and hepatopancreas of the freshwater pearl mussel Margaritifera margaritifera from eight northwestern Russian rivers belonging to the White and Barents Sea basins. Tissue specificity of the lipid composition was observed in the studied tissues; i.e., the hepatopancreas had several times higher contents of TL and dominant lipid classes. A predominance of triacylglycerols among energetic lipids in the hepatopancreas was a distinctive feature of pearl mussels from the Lotta, Sredniy Verman, Mashjoki, and Nautsiyoki rivers, whereas cholesterol esters predominated in mussels from the rivers of the Pyaozero water system, which is further south. Furthermore, the cholesterol/phospholipid ratio (an indicator of the structural and functional state of membranes and a marker of temperature adaptation) was the highest in pearl mussels from the Lotta, Sredniy Verman, Mashjoki, and Nautsiyoki rivers. This may be related to corresponding adaptive changes associated with preparation for the winter period, given a more northern location of the rivers. The results of lipid status analysis in pearl mussels from different habitats may be useful for monitoring studies aimed at preserving and restoring populations of the species in the wild.","PeriodicalId":11351,"journal":{"name":"Doklady Biological Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148668606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plasma Extracellular Vesicles from Glioblastoma Patients Affect Phenotype and Activate Receptor Tyrosine Kinases in Glioblastoma Cells. 胶质母细胞瘤患者的血浆细胞外囊泡影响胶质母细胞瘤细胞的表型并激活受体酪氨酸激酶。

Doklady Biological Sciences Pub Date : 2026-08-03 DOI: 10.1134/S0012496625600800

M L Bychkov, A V Kirichenko, K S Yashin, I A Medyanik, M P Kirpichnikov, E N Lyukmanova

{"title":"Plasma Extracellular Vesicles from Glioblastoma Patients Affect Phenotype and Activate Receptor Tyrosine Kinases in Glioblastoma Cells.","authors":"M L Bychkov, A V Kirichenko, K S Yashin, I A Medyanik, M P Kirpichnikov, E N Lyukmanova","doi":"10.1134/S0012496625600800","DOIUrl":"https://doi.org/10.1134/S0012496625600800","url":null,"abstract":"Extracellular vesicles are important transmitters of oncogenic signals between cancer cells. Despite numerous studies of vesicles from model glioblastoma (GBM) cells, information on the effects of vesicles from GBM patients on the development of model GB cells is lacking. In this study, plasma vesicles from healthy donors (HVs) and GBM patients (GVs) were studied to affect expression of differentiation (GFAP and GLT-1) and stemness (CD9 and CD133) markers and expression and activation of the oncogenic receptor tyrosine kinases EGFR and PDGFRβ in GBM cells (primary GBM011 and model U251 MG cell lines) and rat and human normal astrocytes. GVs reduced GFAP expression in GBM011 cells and stimulated GFAP and CD9 expression in U251 MG cells. GVs increased EGFR and PDGFRβ expression in both GBM cell lines and enhanced EGFR (Y1086) and PDGFRβ (Y751) phosphorylation in U251 MG cells. HVs stimulated CD9 expression in U251 MG cells and PDGFRβ expression in GBM011 cells, but decreased EGFR and PDGFRβ expression in U251 MG cells. Thus, both GVs and HVs influence the GBM phenotype and receptor tyrosine kinase activation.","PeriodicalId":11351,"journal":{"name":"Doklady Biological Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148668548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First Record of Fossil Remains of Pipistrelle (Pipistrellus, Vespertilionidae, Chiroptera) in the Urals. 乌拉尔地区pipistrelre化石遗存的首次记录（pipistrelae, vipertiliiae， chiro翅目）。

Doklady Biological Sciences Pub Date : 2026-08-03 DOI: 10.1134/S0012496626600399

E P Izvarin, A I Ulitko, N G Smirnov

引用次数: 0

Expression of Small Secreted Peptide Genes in Abiotic Stress in Plants Interacting with Plant Growth-Promoting Bacteria. 植物促生长菌与非生物胁迫作用下小肽基因的表达

Doklady Biological Sciences Pub Date : 2026-07-13 DOI: 10.1134/S0012496626600259

A A Makeeva, A S Mamaeva, S K Zavriev

{"title":"Expression of Small Secreted Peptide Genes in Abiotic Stress in Plants Interacting with Plant Growth-Promoting Bacteria.","authors":"A A Makeeva, A S Mamaeva, S K Zavriev","doi":"10.1134/S0012496626600259","DOIUrl":"https://doi.org/10.1134/S0012496626600259","url":null,"abstract":"Small secreted peptides (SSPs) are a promising class of signaling molecules that regulate plant growth, development, and stress responses. Under abiotic stress conditions, SSPs can modulate the physiological and molecular responses, thereby influencing plant adaptation. Based on publicly available transcriptomic data on Arabidopsis thaliana, SSP gene expression was analyzed in plants exposed to dehydration or a higher salinity in combination with inoculation by plant growth-promoting bacteria (PGPBs). Candidate genes involved in plant adaptation to stress conditions upon interaction with PGPBs were thus identified. In salt stress, inoculation with Enterobacter sp. SA187 mitigated stress‑induced suppression of growth‑related genes. In dehydration stress, Aeromonas sp. H1 altered expression of genes regulating stomatal development and the root system, but did not affect SSP expression in mock conditions. A combined effect of stress and PGPBs was found to generate a specific transcriptional response. The response is not a simple overlay of two independent reactions, but depends on both the stress type and the bacterial strain.","PeriodicalId":11351,"journal":{"name":"Doklady Biological Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148435451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Ground Fires on Suppression of Orchids under Severe Technogenic Pollution. 严重技术污染下地面火灾对兰科植物抑制的影响

Doklady Biological Sciences Pub Date : 2026-07-13 DOI: 10.1134/S0012496626600144

D V Veselkin, N B Kuyantseva, D A Zharkova, A G Mumber, S A Lesina

引用次数: 0