{"title":"A New Species of Hapalodectes (Hapalodectidae, Mesonychia) from the Paleocene of Mongolia.","authors":"A V Lopatin","doi":"10.1134/S0012496623700709","DOIUrl":"10.1134/S0012496623700709","url":null,"abstract":"<p><p>Hapalodectes paradux sp. nov. (Hapalodectidae, Mesonychia) is described on the base of the dentary fragment with M<sub>2</sub>-M<sub>3</sub> from the Tsagan-Khushu locality in Mongolia (Upper Paleocene, Naran Bulak Formation, Zhigden Member). The M<sub>2</sub> and M<sub>3</sub> are approximately the same size, with a high protoconid, anteriorly displaced reduced metaconid, anterolingually directed protocristid, very deep posterior notch, narrow talonid, and distinct hypoconid, entoconid and hypoconulid. Based on dental characters, the new species is presumably related to the base of the lineage of Hapalodectes that dispersed to North America at the beginning of the Eocene. Tsagan-Khushu is the only known locality where two species of Hapalodectes co-occur (larger H. dux Lopatin, 2001 and smaller H. paradux sp. nov.).</p>","PeriodicalId":11351,"journal":{"name":"Doklady Biological Sciences","volume":" ","pages":"361-367"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10811070/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41106188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M Yu Cheprasov, G G Boeskorov, G P Novgorodov, A N Tikhonov, L V Grigorieva, E S Boulygina, N V Slobodova, F S Sharko, A V Protopopov, A V Nedoluzhko
{"title":"Mid-holocene Brown Bear (Ursus arctos) from the Bolshoy Lyakhovsky Island (New Siberian Islands).","authors":"M Yu Cheprasov, G G Boeskorov, G P Novgorodov, A N Tikhonov, L V Grigorieva, E S Boulygina, N V Slobodova, F S Sharko, A V Protopopov, A V Nedoluzhko","doi":"10.1134/S0012496623700692","DOIUrl":"10.1134/S0012496623700692","url":null,"abstract":"<p><p>A morphological description is provided for a unique find of a frozen mummified subfossil brown bear (Ursus arctos L., 1758), found for the first time ever. The find is a well-preserved bear carcass of approximately 3500 years in age. Results of computed tomography and DNA testing are discussed.</p>","PeriodicalId":11351,"journal":{"name":"Doklady Biological Sciences","volume":" ","pages":"368-373"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10223283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D Kh Arkhestova, O K Anisimova, E Z Kochieva, A V Shchennikova
{"title":"Expression Levels of Flowering Time Genes (CONZ1, GIGZ1A, GIGZ1B, FKF1A, and FKF1B) in Seedlings under Long-Day Conditions Differentiates Early and Late Zea mays L. Lines.","authors":"D Kh Arkhestova, O K Anisimova, E Z Kochieva, A V Shchennikova","doi":"10.1134/S0012496623700710","DOIUrl":"10.1134/S0012496623700710","url":null,"abstract":"<p><p>Phenophase durations, including the timing of flowering and ripeness, were characterized in 20 inbred lines of the maize Zea mays L. Expression of key flowering initiation genes (CONZ1, GIGZ1a, GIGZ1b, ZmFKF1a, and ZmFKF1b) under long-photoperiod conditions was studied in seedlings of six maize lines that differed in ripeness time. Significantly lower transcription levels of all of the five genes were found in early-ripening lines compared with late-ripening lines. Similar expression patterns were observed for the GIGZ1a and GIGZ1b paralogous genes, while ZmFKF1a significantly predominated in expression over its paralog ZmFKF1b.</p>","PeriodicalId":11351,"journal":{"name":"Doklady Biological Sciences","volume":" ","pages":"378-381"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41114674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L A Kovalchuk, V A Mishchenko, L V Chernaya, V P Snit'ko, V N Bolshakov
{"title":"Functional Activity of the Blood System in Two Migratory Bat Species of the Urals.","authors":"L A Kovalchuk, V A Mishchenko, L V Chernaya, V P Snit'ko, V N Bolshakov","doi":"10.1134/S0012496623700898","DOIUrl":"10.1134/S0012496623700898","url":null,"abstract":"<p><p>Functional activity of the blood system was studied in two migratory Ural species, Vespertilio murinus Linnaeus, 1758 and Pipistrellus nathusii Keyserling et Blasius, 1839. A multivariate nonparametric ANOVA of red blood parameters showed significant interspecific differences (p < 0.05) between the migrating bats and the resident species pond bat. A certain genetically determined multidirectionality in the mobilization of emergency regulation mechanisms of the lymphoid blood system was observed in bats.</p>","PeriodicalId":11351,"journal":{"name":"Doklady Biological Sciences","volume":" ","pages":"S61-S64"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140012417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Diet of the Small Cave Bear Ursus (Spelaearctos) rossicus Borissak, 1930 (Mammalia, Carnivora, Ursidae) As Revealed by <sup>13</sup>C and <sup>15</sup>N Isotope Analyses in Bone Collagen.","authors":"P A Kosintsev, K Yu Konovalova, G V Simonova","doi":"10.1134/S0012496623700825","DOIUrl":"10.1134/S0012496623700825","url":null,"abstract":"<p><p>The <sup>13</sup>C and <sup>15</sup>N isotope contents in bone collagen were analyzed using bones of the small cave bear Ursus (Spelaearctos) rossicus Borissak, 1930 from localities in the Middle and Southern Urals. The bones date from the last interglacial (MIS 5) and glacial (MIS 3) periods. The bones were from males and females aged 3, 4, and >4 years. Sexual, geographical, and chronological differences in <sup>13</sup>C and <sup>15</sup>N contents were studied. Notable gender, geographic, and chronological differences were observed between samples. In the Middle Urals, females led a more predatory lifestyle than males during the interglacial period, and the trophic niches of males and females converged due to an increase in herbivory during the transition to the glacial period. In the Southern Urals, males led a more predatory lifestyle than in the Middle Urals during the interglacial period. The extent of changes in δ<sup>13</sup>C and δ<sup>15</sup>N values in the Southern Urals during the transition was found to correspond to differences between trophic levels.</p>","PeriodicalId":11351,"journal":{"name":"Doklady Biological Sciences","volume":" ","pages":"S14-S18"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139377346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Are Abnormally Large Eastern Bent-Wing Bats, Miniopterus fuliginosus, the Result of a Feature Shift or the Result of Hybridization?","authors":"S V Kruskop, S S Zhukova","doi":"10.1134/S0012496624700911","DOIUrl":"10.1134/S0012496624700911","url":null,"abstract":"<p><p>During a study of morphological variations in bent-wing bats (Miniopterus), we found that specimens originating from the Cao Bang province of Vietnam, identified as M. fuliginosus, were closer in size to the larger species, M. magnater. Mitochondrial gene analysis clearly places these specimens in the former species. At the same time, morphometric analysis of 21 cranial measurements definitely puts these specimens closer to M. magnater and shows their differences from M. fuliginosus samples from different parts of the species range. We assume that, in the low mountains of northern Vietnam and southern China, M. fuliginosus, due to as yet unknown circumstances, occupies the niche of a larger species. However insufficient data on nuclear markers also assume a hybrid origin of this population, with mtDNA introgression from another species.</p>","PeriodicalId":11351,"journal":{"name":"Doklady Biological Sciences","volume":" ","pages":"S65-S71"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140012416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N A Kruglova, D S Komkov, D V Mazurov, M V Shepelev
{"title":"The RRE-Rev Module Has No Effect on the Packaging Efficiency of Cas9 and Gag Proteins into NanoMEDIC Virus-like Particles.","authors":"N A Kruglova, D S Komkov, D V Mazurov, M V Shepelev","doi":"10.1134/S0012496623700886","DOIUrl":"10.1134/S0012496623700886","url":null,"abstract":"<p><p>Delivery of ribonucleoprotein complexes of Cas9 nuclease and guide RNA into target cells with virus-like particles (VLP) is one of the novel methods of genome editing and is suitable for gene therapy of human diseases in the future. The efficiency of genome editing with VLPs depends on the Cas9 packaging into VLPs, the process mediated by the viral Gag protein. To improve the packaging of Cas9 into NanoMEDIC VLPs, plasmid constructs for Cas9 and Gag expression were modified by adding the HIV Rev response element (RRE), which was expected to increase the nuclear export of RRE-containing transcripts into the cytosol via the Rev accessory protein, as described for a Vpr-Cas9-based VLP system. The Cas9 and Gag protein levels in cell lysates were found to increase upon cotransfection with either the Rev-expressing plasmid or the empty control plasmid. The effect was independent of the presence of RRE in the transcript. Moreover, AP21967-induced dimerization of FRB and FKBP12, but not plasmid modification with RRE and/or cotransfection with the Rev-expressing plasmid, was shown to play the major role in Cas9 packaging into NanoMEDIC VLPs. The data indicated that it is impractical to use the RRE-Rev module to enhance the packaging of Cas9 nuclease into VLPs.</p>","PeriodicalId":11351,"journal":{"name":"Doklady Biological Sciences","volume":" ","pages":"S45-S50"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140109620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Sensory Structures of the Carapace of Male Tantulocarida (Crustacea) Are not Homologous to the Lattice Organs of Thecostraca.","authors":"A S Savchenko, G A Kolbasov","doi":"10.1134/S0012496623600161","DOIUrl":"10.1134/S0012496623600161","url":null,"abstract":"<p><p>Ultrastructural studies on the sensory apparatus of male Tantulocarida (Crustacea) have been conducted for the first time. Comparative morphological analysis with the specialized sensory structures of Thecostraca, known as lattice organs, has allowed for conclusions about possible homologies and further clarification of the phylogenetic position of Tantulocarida.</p>","PeriodicalId":11351,"journal":{"name":"Doklady Biological Sciences","volume":" ","pages":"S37-S40"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140109621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Erratum to: Role of Antimicrobial Peptides in Immunity of Parasitic Leeches.","authors":"I A Kaygorodova","doi":"10.1134/S0012496623050022","DOIUrl":"10.1134/S0012496623050022","url":null,"abstract":"","PeriodicalId":11351,"journal":{"name":"Doklady Biological Sciences","volume":"513 1","pages":"405"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10810920/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139563626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Erratum to: Mustela strandi (Mustelidae, Carnivora) from the Early Pleistocene of Crimea.","authors":"D O Gimranov, Q Jiangzuo, A V Lavrov, A V Lopatin","doi":"10.1134/S0012496623050010","DOIUrl":"10.1134/S0012496623050010","url":null,"abstract":"","PeriodicalId":11351,"journal":{"name":"Doklady Biological Sciences","volume":"513 1","pages":"404"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10811147/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139563620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}