S O Medvedeva, O E Cherepanova, I V Petrova, A Yu Teptina
{"title":"Detection and Verification of Hybrid Betula nana L. Plants in the Urals.","authors":"S O Medvedeva, O E Cherepanova, I V Petrova, A Yu Teptina","doi":"10.1134/S0012496624600696","DOIUrl":null,"url":null,"abstract":"<p><p>Hybrid plants were verified in Betula nana populations of the Urals by flow cytometry and were especially numerous in the northern part of the region. ITS1 nucleotide sequences were obtained by Sanger sequencing. Based on their analysis, low differentiation was observed for B. nana and B. pubescens and associated with current and ancestral hybridization processes. Intragenomic polymorphism of ITS1 sequences was assessed using the Illumina technology and detected both B. nana and B. pubescens rDNAs in the genomes of first-generation interspecific hybrids.</p>","PeriodicalId":11351,"journal":{"name":"Doklady Biological Sciences","volume":"521 1","pages":"107-110"},"PeriodicalIF":0.0000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Doklady Biological Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1134/S0012496624600696","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/4/11 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 0
Abstract
Hybrid plants were verified in Betula nana populations of the Urals by flow cytometry and were especially numerous in the northern part of the region. ITS1 nucleotide sequences were obtained by Sanger sequencing. Based on their analysis, low differentiation was observed for B. nana and B. pubescens and associated with current and ancestral hybridization processes. Intragenomic polymorphism of ITS1 sequences was assessed using the Illumina technology and detected both B. nana and B. pubescens rDNAs in the genomes of first-generation interspecific hybrids.
期刊介绍:
Doklady Biological Sciences is a journal that publishes new research in biological sciences of great significance. Initially the journal was a forum of the Russian Academy of Science and published only best contributions from Russia in the form of short articles. Now the journal welcomes submissions from any country in the English or Russian language. Every manuscript must be recommended by Russian or foreign members of the Russian Academy of Sciences.