T I Vlasova, E P Brodovskaya, K S Madonov, V P Ageev, A P Abelova, S I Pinyaev, A P Vlasov
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引用次数: 0
Abstract
The regenerative potential of platelet-rich plasma (PRP) samples from patients with type 1 diabetes mellitus (DM) was studied in vitro. In a series of experiments, PRP was added to the culture medium of human dermal fibroblasts. The cells were tested for metabolic (including reactive oxygen species production), migration, and proliferative activities at several time points; growth factors and exosome levels were assessed in PRP and the medium. A lower proliferative effect, pro-oxidant properties, and toxicity were observed for PRPs from diabetic patients. Namely, treated fibroblasts showed lower metabolic activity, a lower cell viability, and a greater percentage of necrosis in culture, while their migration properties were not impaired. PRPs from diabetic patients exerted a lower stimulatory effect on growth factor secretion by dermal fibroblasts. PRPs from elderly diabetics had the greatest effects.
期刊介绍：
Doklady Biological Sciences is a journal that publishes new research in biological sciences of great significance. Initially the journal was a forum of the Russian Academy of Science and published only best contributions from Russia in the form of short articles. Now the journal welcomes submissions from any country in the English or Russian language. Every manuscript must be recommended by Russian or foreign members of the Russian Academy of Sciences.
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