Diagnostic microbiology and infectious disease最新文献

{"title":"Evaluation of loop mediated isothermal amplification, quantitative real-time PCR, conventional PCR methods for identifying Ascaris lumbricoides in human stool samples","authors":"Mamta Thakur ,&nbsp;Abhishek Mewara ,&nbsp;PVM Lakshmi ,&nbsp;Sucheta Guleria ,&nbsp;Sumeeta Khurana","doi":"10.1016/j.diagmicrobio.2025.116808","DOIUrl":"10.1016/j.diagmicrobio.2025.116808","url":null,"abstract":"<div><div>Ascariasis, caused by <em>Ascaris lumbricoides</em>, is a widespread parasitic infection. Traditional diagnostic methods, such as microscopy, can miss infections with low worm burdens, leading to false negatives. This study compares four diagnostic methods—microscopy, conventional PCR, real-time PCR, and loop-mediated isothermal amplification (LAMP)—for detecting <em>A. lumbricoides</em> in 400 stool samples from children aged 2-16. Microscopy methods (direct wet mount, Kato-Katz, and concentration) detected 17, 23, and 21 positive samples, respectively. Molecular techniques identified 23 positive samples by conventional PCR, 29 by real-time PCR, and 25 by LAMP. Notably, real-time PCR detected two samples missed by microscopy, while conventional PCR failed to detect three samples positive by real-time PCR and LAMP. In limit-of-detection assays, conventional PCR detected <em>A. lumbricoides</em> DNA down to 150 pg, while qPCR and LAMP could detect as low as 15 fg. For egg number analysis, conventional PCR detected DNA from 100 eggs, while qPCR and LAMP identified DNA from just 10 eggs. The methods specifically targeted <em>A. lumbricoides</em>, without cross-reacting with other co-occurring parasites. Sensitivity and specificity analysis revealed that microscopy had sensitivities of 81.3 %, while conventional PCR, qPCR, and LAMP had sensitivities of 81.1 %, 99.2 %, and 88.1 %, respectively. Microscopy and conventional PCR had 100 % specificity, while qPCR and LAMP had 99.2 % and 99.9 % specificity. While Kato-Katz is advantageous for detecting active infections, molecular techniques, particularly LAMP's field applicability in resource-limited settings makes it a promising tool for surveillance and control of low-intensity infections.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 3","pages":"Article 116808"},"PeriodicalIF":2.1,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anterior nasal swabs compared to nasopharyngeal swabs for detection of respiratory viruses in children","authors":"Abigail P. Kietzman ,&nbsp;Nicole Neeley ,&nbsp;Rangaraj Selvarangan ,&nbsp;Dithi Banerjee ,&nbsp;Jennifer L. Goldman ,&nbsp;Jennifer E. Schuster","doi":"10.1016/j.diagmicrobio.2025.116821","DOIUrl":"10.1016/j.diagmicrobio.2025.116821","url":null,"abstract":"<div><div>Respiratory viral testing often uses invasive nasopharyngeal (NP) swabs, which can be painful and require trained personnel. Anterior nasal swabs (NS) are less invasive and can be self-collected. The sensitivity of NS compared to NP specimens for detecting multiple respiratory viruses in children are not well described. Hospitalized children in Kansas City, MO, from January 2023 to February 2024, who had NP specimens obtained for standard of care multiplex respiratory viral testing in the previous 72 h, were enrolled. NS specimens were collected and tested alongside salvaged NP specimens for adenovirus, seasonal coronaviruses, human metapneumovirus, respiratory syncytial virus, influenza, rhinovirus/enterovirus, SARS-CoV-2, and parainfluenza viruses using multiplex molecular testing. Concordance, sensitivity, and specificity of NS compared to NP specimens were assessed. A total of 147 paired NP/NS specimens were analyzed. Overall, 114 (77.6 %) NP/NS pairs were concordant, including 86 (58.5 %) virus-positive and 28 (19.1 %) virus-negative pairs. NS sensitivity was 84.3 % compared to NP, increasing to 95.7 % when collected within 24 h of NP specimens. Sensitivity for seasonal coronavirus was poor (36.4 %), but was over 75 % for other viruses, and 100 % for adenovirus, influenza, parainfluenza, RSV, and SARS-CoV-2 within 24 h of NP specimens. Virus cycle threshold counts were similar among paired specimens. NS specimens showed good concordance with NP specimens and high sensitivity for most viruses, except seasonal coronavirus. NS testing may enable respiratory virus monitoring outside medical settings.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 3","pages":"Article 116821"},"PeriodicalIF":2.1,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143705871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnosis of Cladophialophora bantiana cerebral infection by clinical metagenomics","authors":"Wanessa Rolando Roselli ,&nbsp;Fernando Franco de Oliveira Miraglia ,&nbsp;Fernanda de Mello Malta ,&nbsp;Gustavo Bruniera Peres Fernandes ,&nbsp;Guilherme Carvalhal Ribas ,&nbsp;Clécio de Oliveira Godeiro Júnior ,&nbsp;Deyvid Emanuel Amgarten ,&nbsp;Fabiane Camargo G Nunes ,&nbsp;Mario Trindade ,&nbsp;João Nóbrega de Almeida Junior ,&nbsp;Marines Dalla Valle Martino ,&nbsp;Rubia Anita Ferraz Santana ,&nbsp;João Renato Rebello Pinho ,&nbsp;André Mario Doi","doi":"10.1016/j.diagmicrobio.2025.116822","DOIUrl":"10.1016/j.diagmicrobio.2025.116822","url":null,"abstract":"<div><h3>Purpose</h3><div><em>Cladophialophora bantiana</em> is a rare cause of severe central nervous system phaeohyphomycosis.</div></div><div><h3>Results</h3><div>A 44-year-old woman from Northeastern Brazil with a history of scotomas, mental confusion, and impaired coordination presented Magnetic Resonance Imaging with vasogenic edema and nodular enhancement in the left occipital lobe. Brain biopsy showed neutrophilic exudate with eosinophils, macrophages, giant multinucleated cells, and septate hyphae with a brown-colored birefringent wall and acute angle branching. Metagenomic analysis of the biopsy's total RNA revealed the presence of RNA sequences highly similari to <em>C. bantiana. C</em>ulture confirmed the presence of olivaceous-gray suede-like to floccose colonies, with septate dematiaceous hyphae and long conidia chains from undifferentiated conidiophores, consistent with <em>C. bantiana</em>.</div></div><div><h3>Conclusions</h3><div>This case highlights the potential of metagenomic testing as a tool for early diagnosis of infections caused by uncommon fungal pathogens. To our knowledge, this is the first report of <em>C. bantiana</em> central nervous system infection in Brazil.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 3","pages":"Article 116822"},"PeriodicalIF":2.1,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143686299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An expanding threat; a case of encephalitis caused by West Nile Virus in the Madrid Region (Spain)","authors":"Víctor Antón Berenguer ,&nbsp;Sara Gómez de Frutos ,&nbsp;Clara Peiró Villalba ,&nbsp;Cristina Trevino-Peinado ,&nbsp;Carlota Ballester Martínez ,&nbsp;Antonio Nicolás Ruiz Benito ,&nbsp;María Dolores Martín Rodrigo ,&nbsp;Sara María Quevedo Soriano ,&nbsp;Gema Cenzual Álvarez ,&nbsp;Sara Hernández Egido ,&nbsp;Roberto López-Blanco","doi":"10.1016/j.diagmicrobio.2025.116810","DOIUrl":"10.1016/j.diagmicrobio.2025.116810","url":null,"abstract":"<div><div>West Nile Virus (WNV) has increasingly impacted public health in Europe. We present a WNV-encephalitis case in a young healthy patient, an atypical population for neuroinvasive forms. Although likely imported, WNV circulation in new areas highlights the need for awareness of WNV beyond classical endemical areas.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 3","pages":"Article 116810"},"PeriodicalIF":2.1,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143705872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization of autochthonous Hepatitis E virus detected from a human acute infection in the Calabria Region, Southern Italy","authors":"Nadia Marascio ,&nbsp;Marta Pantanella ,&nbsp;Grazia Pavia ,&nbsp;Chiara Mazzei ,&nbsp;Sebastiano Di Salvo ,&nbsp;Francesca Trimboli ,&nbsp;Giorgio S. Barreca ,&nbsp;Angelo G. Lamberti ,&nbsp;Massimo De Siena ,&nbsp;Tiziana Gravina ,&nbsp;Giovanni Matera ,&nbsp;Angela Quirino","doi":"10.1016/j.diagmicrobio.2025.116807","DOIUrl":"10.1016/j.diagmicrobio.2025.116807","url":null,"abstract":"<div><div>Herein, we reported the molecular characterization of HEV autochthonous strain from an immunocompetent patient. The HEV was classified as subtype 3c and displayed the V1479I ribavirin resistance mutation. The phylogenetic tree analysis showed two statistically supported clusters, including viral strains from symptomatic patients, without severe disease, and meat products.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 3","pages":"Article 116807"},"PeriodicalIF":2.1,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143686298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global impact of hMPV virus: Transmission, pathogenesis, diagnostic and treatment","authors":"Sejal Porwal ,&nbsp;Rishabha Malviya ,&nbsp;Sathvik Belagodu Sridhar ,&nbsp;Javedh Shareef ,&nbsp;Tarun Wadhwa","doi":"10.1016/j.diagmicrobio.2025.116809","DOIUrl":"10.1016/j.diagmicrobio.2025.116809","url":null,"abstract":"<div><div>The Human Metapneumovirus (hMPV), a member of the <em>Pneumovirinae</em> subfamily, is a substantial cause of acute lower respiratory infections, notably in young children, the elderly, and immunocompromised patients. It was first identified in 2001, hMPV has displayed a seasonal pattern of infection, with symptoms ranging from moderate to severe respiratory disease. This study investigates the worldwide effect of hMPV, concentrating on its transmission, etiology, diagnostics, and treatment techniques, underlining the need for better public health measures. hMPV is spread by respiratory droplets, with a normal incubation period of 3–5 days. The virus induces an immune response characterized by pro-inflammatory cytokines, leading to respiratory symptoms and probable tissue damage. Diagnostic breakthroughs, including RT-qPCR and mNGS, have enhanced detection sensitivity. However, therapy is generally supportive, with potential breakthroughs in mRNA vaccines targeting hMPV fusion proteins. Current clinical studies evaluate the effectiveness and safety of these new vaccinations, which might pave the road for effective prevention.</div><div>Despite tremendous gains in understanding hMPV, there remains a crucial need for targeted antiviral treatments and vaccines to minimize its worldwide health impact.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 3","pages":"Article 116809"},"PeriodicalIF":2.1,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143686300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the susceptibility profiles and in vitro combinations of caspofungin, itraconazole, fluconazole, voriconazole, clotrimazole, and amphotericin B against clinical isolates causing fungal keratitis","authors":"Shaghayegh Moghadam ,&nbsp;Hossein Zarrinfar ,&nbsp;Ali Naseri ,&nbsp;Javad Sadeghi ,&nbsp;Mohammad Javad Najafzadeh ,&nbsp;Rozhan Heydarian","doi":"10.1016/j.diagmicrobio.2025.116806","DOIUrl":"10.1016/j.diagmicrobio.2025.116806","url":null,"abstract":"<div><div>Fungal keratitis is a global cause of blindness, highlighting the need to assess antifungal treatment efficacy before clinical use. This study evaluates the synergistic and antagonistic effects of antifungal agents on fungal keratitis isolates. Susceptibility testing of 35 corneal isolates including <em>Aspergillus</em> spp., <em>Neocosmospora</em> spp., <em>Candida albicans, Penicillium chrysogenum</em> was performed with broth microdilution and checkerboard methods to test six antifungal agents (caspofungin, itraconazole, fluconazole, voriconazole, amphotericin B, clotrimazole). Among <em>Aspergillus</em> spp., the MIC ranges were reported as 0.01–1, 0.5–16, 32–64, 0.06–16, 0.125–8, and 0.25–4 µg/mL for caspofungin, amphotericin B, fluconazole, itraconazole, voriconazole, and clotrimazole, respectively. For <em>Fusarium/Neocosmospora</em> spp., these ranges varied to 0.01–8, 0.125–4, 64, 0.03–16, 0.25–8, and 0.5–8 µg/mL for the same drugs. In <em>Candida</em> spp., the MIC ranges were 0.01–0.03, 0.25, 64, ≥16, ≥16, and 0.5–1 µg/mL for the same antifungal agents. Notably, the combination of caspofungin and amphotericin B exhibited 100% synergistic effects against <em>Aspergillus</em> spp., while combinations of clotrimazole, fluconazole, or voriconazole with amphotericin B showed a 50% synergistic effect against <em>Fusarium/Neocosmospora</em> spp. Furthermore, fluconazole combined with voriconazole or itraconazole, as well as voriconazole and itraconazole, demonstrated 100% synergism against <em>Candida</em> spp. The results indicate that caspofungin is the most effective antifungal agent, while fluconazole exhibited the highest rates of resistance. The combination of caspofungin and amphotericin B proved most effective against <em>Aspergillus</em> spp., azole compounds combined with amphotericin B were most effective against <em>Fusarium/ Neocosmospora</em> spp., and combinations of fluconazole with voriconazole or itraconazole and voriconazole and itraconazole demonstrated synergistic effects against <em>Candida</em> spp.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 3","pages":"Article 116806"},"PeriodicalIF":2.1,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143686302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of PERFORMANCE of the IMMY and Dynamiker Aspergillus Galactomannan lateral flow assays for the diagnosis of invasive Aspergillosis","authors":"Chao-Ju Chen ,&nbsp;Jia-Hua Wu ,&nbsp;Ho-Yin Huang ,&nbsp;Po-Liang Lu ,&nbsp;Hung-Pin Tu ,&nbsp;Shang-Yi Lin","doi":"10.1016/j.diagmicrobio.2025.116805","DOIUrl":"10.1016/j.diagmicrobio.2025.116805","url":null,"abstract":"<div><div>Current laboratory diagnostic techniques for invasive aspergillosis (IA), such as fungal cultures and enzyme immunoassay (EIA) for the galactomannan antigen (GM), are limited by their low sensitivity, labor-intensive nature, and prolonged processing times. Some lateral flow assays (LFAs) have been developed for the diagnosis of IA; however, clinical validation and comparative studies are lacking. This study assessed the diagnostic performance of two CE-marked GM-LFAs, IMMY-GM-LFA and QuicGM-LFA. This retrospective study comprised 44 bronchoalveolar lavage fluid (BALF) and 127 serum samples obtained from 137 patients, 17 (9.9 %) of whom had proven or probable IA categorized using the revised EORTC/MSG criteria, between September 2020 and March 2021. The correlation coefficient between the IMMY-GM-LFA and GM-EIA was 0.95, while that between the QuicGM-LFA and GM-EIA was 0.90. Both LFAs GM indexes were significantly higher among those with proven/probable IA versus those with no IA and possible IA cases (BALF: 7.92 vs 0.18 and 14.05 vs 0.33; serum: 1.86 vs 0.26 and 5.75 vs 0.38 by IMMY and QuicGM-LFA, respectively. All <em>p</em>&lt;0.05). In BALF samples, the area under the curve (AUC) for IMMY-GM-LFA and QuicGM-LFA were 0.93 and 0.96, respectively, with optimized GM cut-offs established at 1.2 for IMMY-GM-LFA and 0.78 for QuicGM-LFA. In serum samples, the AUC for IMMY-GM-LFA was 1.0, and for QuicGM-LFA was 0.9, with optimized cut-offs of 0.7 and 0.76, respectively. In conclusion, this study demonstrated that IMMY-GM and QuicGM-LFAs offer comparable alternatives to the Bio-Rad GM-EIA when testing BALF and serum samples with optimized thresholds.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 3","pages":"Article 116805"},"PeriodicalIF":2.1,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143686303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The evolving epidemiology of antimicrobial resistance of ESKAPE pathogens isolated in the intensive care unit of a Greek university hospital","authors":"Sofia Maraki ,&nbsp;Viktoria Eirini Mavromanolaki ,&nbsp;Anna Kasimati ,&nbsp;Evangelia Iliaki-Giannakoudaki ,&nbsp;Dimitra Stafylaki","doi":"10.1016/j.diagmicrobio.2025.116804","DOIUrl":"10.1016/j.diagmicrobio.2025.116804","url":null,"abstract":"<div><h3>Background</h3><div>The ESKAPE group (<em>Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter</em>) is a major cause of life-threatening infections in Intensive Care Unit (ICU) critically ill patients accounting for significant morbidity and mortality. Management of infections by ESKAPE pathogens is complicated due to the remarkable rise in the rates of multidrug resistance (MDR) to most antimicrobial agents. This study investigated the trends of prevalence and antimicrobial resistance profiles of the ESKAPE pathogens isolated from clinical specimens of adult ICU patients.</div></div><div><h3>Methods</h3><div>All ESKAPE isolates collected from clinical specimens of ICU patients during the years 2013-2022 were processed according to routine methods. Identification of the bacterial isolates was performed by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry and antimicrobial susceptibility testing by Vitek 2 system.</div></div><div><h3>Results</h3><div>During the ten-year period, a total of 6,132 ESKAPE strains were isolated from 5,338 samples of 1,792 ICU patients. <em>A. baumannii</em> was the most prevalent microorganism, followed by <em>P. aeruginosa</em>, and <em>K. pneumoniae</em>. High resistance to carbapenems was detected for <em>A. baumannii</em> (96.7 %) and <em>K. pneumoniae</em> (57.4 %). Methicillin-resistant were 39.1 % of <em>S. aureus</em> and vancomycin-resistant 38.7 % of the <em>E. faecium</em> isolates. MDR were characterized 39 % of <em>K. pneumoniae</em> and 13.1 % of <em>P. aeruginosa</em> strains, while 19.7 % of <em>A. baumannii</em> were pandrug resistant.</div></div><div><h3>Conclusions</h3><div>An increased antimicrobial resistance among ESKAPE bacteria was found in our ICU setting. Enhanced antimicrobial surveillance, strict implementation of stewardship programmes and infection control practices are crucial to reduce AMR and develop management strategies to optimize outcomes.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 3","pages":"Article 116804"},"PeriodicalIF":2.1,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bloodstream infection caused by sarcina ventriculi:Case report","authors":"Dan Gou ,&nbsp;Pu Li ,&nbsp;Guangli Luo ,&nbsp;Bianqin Guo","doi":"10.1016/j.diagmicrobio.2025.116803","DOIUrl":"10.1016/j.diagmicrobio.2025.116803","url":null,"abstract":"<div><div><em>Sarcina ventriculi</em> is a gram-positive anaerobic coccus that commonly associated with various gastrointestinal diseases. Here, we report a complete case of bloodstream infection caused by <em>Sarcina ventriculi</em> for the first time. A 60-year-old male patient diagnosed with sigmoid colon adenocarcinoma who had undergone multiple chemotherapy and radiotherapy. The patient underwent blood culture sampling due to fever and the pathogen identified as <em>Sarcina ventriculi</em> through 16S rRNA sequencing analysis. <em>Sarcina ventriculi</em> has a unique arrangement and cell wall structure, which can easily be mistaken for a fungus. This case reveals the difficulties in the diagnosis and treatment of <em>Sarcina ventriculi</em> infection. It can be served as a reference for clinicians and microbiologists faced with similar diagnostic and treatment challenges in the future. Meanwhile, we call for increased attention to <em>Sarcina ventriculi</em> which may also be the late complication of gastrointestinal malignancies.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 3","pages":"Article 116803"},"PeriodicalIF":2.1,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143669383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}