Diagnostic microbiology and infectious disease最新文献_第7页

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IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-02-26 DOI: 10.1016/S0732-8893(25)00079-3

引用次数: 0

Diagnostic challenges in detecting blaIMP-22 carbapenemase in Klebsiella pneumoniae

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-02-25 DOI: 10.1016/j.diagmicrobio.2025.116772

Rita Martins , Maria Antónia Read , Isabel Neves , Luísa Peixe , Valquíria Alves , Ângela Novais

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Comparison of three different molecular biology assays (AllPlexTM H. pylori & ClariR assay, Amplidiag® H. pylori + ClariR and RIDA®GENE Helicobacter pylori) to detect Helicobacter pylori and clarithromycin resistance in stool samples

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-02-25 DOI: 10.1016/j.diagmicrobio.2025.116771

Anne Donnars , Julie Cremniter , Chloé Plouzeau , Anthony Michaud , Lauranne Broutin , Christophe Burucoa , Maxime Pichon

{"title":"Comparison of three different molecular biology assays (AllPlexTM H. pylori & ClariR assay, Amplidiag® H. pylori + ClariR and RIDA®GENE Helicobacter pylori) to detect Helicobacter pylori and clarithromycin resistance in stool samples","authors":"Anne Donnars , Julie Cremniter , Chloé Plouzeau , Anthony Michaud , Lauranne Broutin , Christophe Burucoa , Maxime Pichon","doi":"10.1016/j.diagmicrobio.2025.116771","DOIUrl":"10.1016/j.diagmicrobio.2025.116771","url":null,"abstract":"<div><div><em>Helicobacter pylori</em> detection and susceptibility profile using feces could optimize guided therapy, when endoscopy is not necessary. This study evaluated the performances of three tests: AllPlex<em>H.pylori</em>&ClariR, RIDAGENE<em>Helicobacterpylori</em> and Amplidiag<em>H.pylori</em>+ClariR assays on stool samples. Stool samples from a documented cohort (50 positive and 25 negative) were analyzed. The gold standard was a composite based on PCR targeting <em>H. pylori</em> and 23S rDNA mutations (A2142C, A2142G, A2143G) on gastric biopsies; and biopsy culture for <em>H.pylori</em> and susceptibility testing. For AllPlex, RidaGene and Amplidiag assays respectively: 55 (73.3%), 75 (100%), 54 (72%) samples could be analyzed; (for detection of <em>H. pylori)</em>, sensitivity was 36% (95%CI]28;52%[); 32% (95%CI]21;46%[) and 93% (95%CI]87;100%[); specificity was 100% (95%CI]81;100%[), 83% (95%CI]68;91%[) and 57% (95%CI]33;79%[). (for the Clarithromycin resistance), sensitivity was 18% (95%CI]5;48%[), 25% (95%CI]9;53%[) and 67% (95%CI]39;86%[); specificity was 100% (95%CI]92;100%[). 92% (95%CI]83;97%[) and 97% (95%CI]89;99%[). Innovative technologies could become invaluable tools for mass testing after improvement.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 2","pages":"Article 116771"},"PeriodicalIF":2.1,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143548220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Performance of the cobas 5800 System for Hepatitis B virus DNA and Hepatitis C virus RNA quantification

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-02-25 DOI: 10.1016/j.diagmicrobio.2025.116753

Erwan Vo-Quang , Delphine Rosse , Valérie Ortonne , Olivia Garrigou , Patrick Ingiliz , Vincent Leroy , Jean-Michel Pawlotsky , Stéphane Chevaliez

{"title":"Performance of the cobas 5800 System for Hepatitis B virus DNA and Hepatitis C virus RNA quantification","authors":"Erwan Vo-Quang , Delphine Rosse , Valérie Ortonne , Olivia Garrigou , Patrick Ingiliz , Vincent Leroy , Jean-Michel Pawlotsky , Stéphane Chevaliez","doi":"10.1016/j.diagmicrobio.2025.116753","DOIUrl":"10.1016/j.diagmicrobio.2025.116753","url":null,"abstract":"<div><div>Hepatitis B and C infections are an underdiagnosed global health problem. Measurement of HBV DNA or HCV RNA levels using nucleic acid-based molecular diagnostic assays has been established as the standard of care for assessing diagnosis, guiding the treatment decision, and evaluating responses to antiviral therapy.</div><div>In the present study, we examined the performance of the cobas 5800 System for HBV DNA and HCV RNA quantification in a large series of patients chronically infected.</div><div>Specificity of the cobas HBV and HCV Tests on the 5800 System was high (99.1 % and 100 %, respectively). Linearity using the AcroMetrix panels was excellent. Repeatability and intermediate precision coefficients of variation were within 5 %. Of the 334 clinical specimens tested in parallel on the cobas 5800 and cobas 4800 Systems for HBV and the m2000 RealTime or Alinity m Systems for HCV, only 12 (3.6 %) yielded discrepant results that were at or near the limit of quantification of the cobas 5800 assays. The correlation between viral load results was extremely high, and only weak bias were observed across the entire range of concentrations tested without clinical impact in patients who are eligible for antiviral therapy.</div><div>This comparison study demonstrated equivalent performance of the new cobas 5800 System compared with other molecular platforms widely used in clinical practice for HBV DNA and HCV RNA quantification. The cobas 5800 System can be confidently used in clinical practice. A few clinical specimens with low viral loads may be missed. Further studies are warranted to confirm or refute this finding.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 2","pages":"Article 116753"},"PeriodicalIF":2.1,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143527494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Secondary syphilis with oropharyngeal and skin lesions confirmed by PCR mimicking chicken pox or mpox in a patient with HIV

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-02-21 DOI: 10.1016/j.diagmicrobio.2025.116765

Dong-Min Kim , Do-Yeong Kim , Jun-Won Seo , Da Young Kim , Na Ra Yun , You Mi Lee , Choon-Mee Kim

{"title":"Secondary syphilis with oropharyngeal and skin lesions confirmed by PCR mimicking chicken pox or mpox in a patient with HIV","authors":"Dong-Min Kim , Do-Yeong Kim , Jun-Won Seo , Da Young Kim , Na Ra Yun , You Mi Lee , Choon-Mee Kim","doi":"10.1016/j.diagmicrobio.2025.116765","DOIUrl":"10.1016/j.diagmicrobio.2025.116765","url":null,"abstract":"<div><div>Syphilis, caused by <em>Treponema pallidum</em>, can mimic infections like chickenpox and mpox, especially in patients with HIV, making diagnosis challenging. This case involved a 30-year-old HIV-positive man who presented with fever, sore throat, and widespread rash. He was initially suspected to have a viral illness and was therefore treated with acyclovir. However, his symptoms did not improve, so further tests were performed. PCR analysis of skin and lymph node biopsies confirmed <em>T. pallidum</em> as the causative agent. The patient had a known penicillin allergy and was therefore treated with doxycycline, which resolved his symptoms. Secondary syphilis often presents with diverse symptoms, including skin rashes, lymphadenopathy, and oropharyngeal involvement, which can easily be mistaken for other infections. This case emphasizes the importance of considering syphilis in the differential diagnosis of HIV patients with similar symptoms and highlights the critical role of PCR testing in providing an accurate diagnosis and guiding appropriate treatment.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 2","pages":"Article 116765"},"PeriodicalIF":2.1,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143527495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Performance evaluation of NeuMoDx 96, a random access system for CMV DNA quantitation

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-02-21 DOI: 10.1016/j.diagmicrobio.2025.116746

Priyanka Sharma , Ekta Gupta , Guresh Kumar , Bhawna Narula , Gaurav Singh , Reshu Agarwal

{"title":"Performance evaluation of NeuMoDx 96, a random access system for CMV DNA quantitation","authors":"Priyanka Sharma , Ekta Gupta , Guresh Kumar , Bhawna Narula , Gaurav Singh , Reshu Agarwal","doi":"10.1016/j.diagmicrobio.2025.116746","DOIUrl":"10.1016/j.diagmicrobio.2025.116746","url":null,"abstract":"<div><div>Cytomegalovirus (CMV) poses a significant risk to immunocompromised individuals, particularly in post-transplant settings, leading to substantial morbidity and occasional mortality. Early and accurate quantification of CMV DNA is essential for timely intervention. This retrospective study evaluated the performance of the NeuMoDx 96 platform, a random-access CMV assay, compared to the COBAS® AmpliPrep/COBAS® TaqMan® CMV assay, a batch testing platform. Using 104 archived once thawed plasma samples and the WHO International Standard for CMV, the NeuMoDx assay demonstrated 98.2 % sensitivity, 100 % specificity, and 99.04 % diagnostic accuracy compared to the COBAS® assay. Excellent concordance was observed with the WHO standard. These findings suggest that the NeuMoDx 96 platform offers a cost-effective and rapid method for CMV detection, potentially improving clinical decision-making in critically ill patients. Further research on similar assays across diverse clinical settings is essential to enhance the early diagnosis and treatment of CMV infections.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 1","pages":"Article 116746"},"PeriodicalIF":2.1,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143549709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel flow through assay and smartphone application based prototype for point-of-care diagnosis of tuberculosis

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-02-20 DOI: 10.1016/j.diagmicrobio.2025.116766

Joanne Hacking , Vanessa Valerie Gwenin , Mark Stephen Baird , Mohmmad Rizwan , Christopher David Gwenin

{"title":"A novel flow through assay and smartphone application based prototype for point-of-care diagnosis of tuberculosis","authors":"Joanne Hacking , Vanessa Valerie Gwenin , Mark Stephen Baird , Mohmmad Rizwan , Christopher David Gwenin","doi":"10.1016/j.diagmicrobio.2025.116766","DOIUrl":"10.1016/j.diagmicrobio.2025.116766","url":null,"abstract":"<div><div>Affordable point-of-care test sensors with automated result recording are essential for reducing undetected tuberculosis cases in remote, resource-limited areas. Therefore, this study addresses this need with three key aims. First, we aimed to lower the costs of a patented flow-through assay (Kit and method: WO2016/024116A1) by developing an in-house method for producing antibody-coated gold nanoparticles (anti-IgG-AuNPs). These anti-IgG-AuNPs demonstrated specific binding with performance comparable to existing antibody-capped gold nanoparticles. The second aim was to transform the flow-through assay into a multi-disease screening tool by incorporating multiple antigen test spots. A newly designed wax-printed background allows for simultaneous testing of up to five antigens, delivering results within 15 min at the point-of-care, while also reducing assay costs by 70 %. Lastly, we developed a smartphone application (RAP-TBS) to provide quantitative analysis of the flow-through assay results. This user-friendly app requires no additional hardware and addresses the limitations of subjective visual interpretation.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 1","pages":"Article 116766"},"PeriodicalIF":2.1,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143549710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mixed fungal infection of Actinomucor elegans and Aspergillus fumigatus in a person with severe fever with thrombocytopenia syndrome

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-02-15 DOI: 10.1016/j.diagmicrobio.2025.116750

Lu Ren , Zhen Liu , Jian Wang , Yuan Su

{"title":"Mixed fungal infection of Actinomucor elegans and Aspergillus fumigatus in a person with severe fever with thrombocytopenia syndrome","authors":"Lu Ren , Zhen Liu , Jian Wang , Yuan Su","doi":"10.1016/j.diagmicrobio.2025.116750","DOIUrl":"10.1016/j.diagmicrobio.2025.116750","url":null,"abstract":"<div><div>In this report, a 69-year-old man with fever, thrombocytopenia, and hepatic and renal dysfunction presented to our hospital. His disease progression was rapid, and he became unconscious. The patient was diagnosed with severe fever with thrombocytopenia syndrome (SFTS). However, his inflammation markers continued to rise until a mixed fungal infection was detected. Metagenomic next-generation sequencing of blood detected elevated sequence numbers for <em>Actinomucor elegans</em> and <em>Aspergillus fumigatus</em>. The culture of the bronchoalveolar lavage fluid were positive for <em>Aspergillus fumigatus</em> and <em>Escherichia coli</em>. Infection was controlled after adjusting the antifungal regimens against <em>Actinomucor elegans</em>. The patient's condition gradually improved. This patient was diagnosed with possible mucormycosis. Reports of Mucorales infection associated with SFTS are rare. We hope that this case report will draw the attention of physicians in the area where SFTS occurs to figure out if patients with SFTS are susceptible to mucormycosis.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 1","pages":"Article 116750"},"PeriodicalIF":2.1,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143463396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation of nontuberculous mycobacteria and Nocardia via mycobacterial culture from pulmonary specimens

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-02-15 DOI: 10.1016/j.diagmicrobio.2025.116752

Hongli Sun , Wenjing Liu , Lingli Liu , Xuefeng Sun

{"title":"Isolation of nontuberculous mycobacteria and Nocardia via mycobacterial culture from pulmonary specimens","authors":"Hongli Sun , Wenjing Liu , Lingli Liu , Xuefeng Sun","doi":"10.1016/j.diagmicrobio.2025.116752","DOIUrl":"10.1016/j.diagmicrobio.2025.116752","url":null,"abstract":"<div><div><em>Nontuberculous mycobacteria</em> (NTM) and <em>Nocardia</em> are often isolated via mycobacterial culture, yet their prevalence in various pulmonary specimens is uncertain. We retrieved mycobacterial culture results from pulmonary specimens from a tertiary hospital in China between January 2017 and July 2021. The prevalence of non-<em>Mycobacterium tuberculosis</em> (non-Mtb, including NTM and <em>Nocardia</em>) has surpassed Mtb, accounting for 1.7 times the rate of Mtb among all isolates. The frequency of non-Mtb positivity in sputum specimens was significantly higher compared to that in bronchoscopy specimens (3.7% vs 1.9%, <em>P</em> < 0.001). Furthermore, NTM accounted for 59.8% of all Mycobacterium isolates and 42.1% of patients with positive acid-fast staining. Multivariate logistic regression analysis identified several risk factors significantly associated with non-Mtb isolates compared to Mtb, including age over 45 years (adjusted odds ratio [aOR] 2.27), bronchiectasis (aOR 4.52), pulmonary interstitial disorder (aOR 2.11), chronic obstructive pulmonary disease (aOR 1.94), and non-diabetes (aOR 2.10).</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"111 4","pages":"Article 116752"},"PeriodicalIF":2.1,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143421413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of bacterial pathogens directly from synovial fluids using digital PCR: A proof of concept study

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-02-15 DOI: 10.1016/j.diagmicrobio.2025.116749

Corrie R Belanger , Kerstin Locher , Billie Velapatiño , Marthe K Charles

引用次数: 0