Diagnostic microbiology and infectious disease最新文献

{"title":"Weak phospholipase-positive Listeria monocytogenes: a challenge for the diagnostic laboratory","authors":"Marc J.A. Stevens ,&nbsp;Joseph Wambui ,&nbsp;Sabrina Corti ,&nbsp;Magdalena Nüesch-Inderbinen ,&nbsp;Roger Stephan","doi":"10.1016/j.diagmicrobio.2025.116802","DOIUrl":"10.1016/j.diagmicrobio.2025.116802","url":null,"abstract":"<div><div><em>Listeria monocytogenes</em> isolates from environmental samples of a food producing company harboured a C424G mutation in the <em>prfA</em> gene that results in a G141A substitution in PrfA. This mutation results in strongly decreased phospholipase activity and is therefore challenging for culture-based detection of <em>L. monocytogenes</em> and may even lead to misidentification.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 3","pages":"Article 116802"},"PeriodicalIF":2.1,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143679263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lymphocutaneous sporotrichosis complicated by pseudoepitheliomatous hyperplasia: A case report","authors":"Thanachote Kamalapirat,&nbsp;Sirin Apichonbancha,&nbsp;Warangkana Tonaree","doi":"10.1016/j.diagmicrobio.2025.116791","DOIUrl":"10.1016/j.diagmicrobio.2025.116791","url":null,"abstract":"<div><div>A 63-year-old Thai woman, owner of 14 cats, presented with chronic skin lesions initially misdiagnosed as cellulitis. Further investigation revealed lymphocutaneous sporotrichosis caused by <em>Sporothrix schenckii</em>. Histopathology of one lesion unexpectedly demonstrated pseudoepitheliomatous hyperplasia (PEH), a reactive epidermal proliferation that can clinically and histopathologically mimic squamous cell carcinoma (SCC). The patient was treated with itraconazole for the fungal infection, and surgical debridement was performed due to suspicion of malignancy. Diagnostic tools included fungal culture and detailed histopathology, emphasizing the importance of thorough evaluation of persistent cutaneous lesions, especially in individuals with animal exposure. This case highlights the rarity of hyperplastic or verrucous variants in chronic fungal infections and underscores the need to distinguish benign PEH from true malignancy. Timely intervention with antifungal therapy and appropriate surgical management resulted in a favorable outcome.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 3","pages":"Article 116791"},"PeriodicalIF":2.1,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Field deployment of the mobile suitcase laboratory for rapid detection of Mpox virus in the Democratic Republic of the Congo","authors":"Rea Maja Kobialka ,&nbsp;Arianna Ceruti ,&nbsp;Padra Malwengo-Kasongo ,&nbsp;Patient Okitale-Talunda ,&nbsp;Yannick Munyeku-Bazitama ,&nbsp;Martin Faye ,&nbsp;Uwe Truyen ,&nbsp;Ahmed Abd El Wahed ,&nbsp;Manfred Weidmann ,&nbsp;Sheila Makiala-Mandanda","doi":"10.1016/j.diagmicrobio.2025.116800","DOIUrl":"10.1016/j.diagmicrobio.2025.116800","url":null,"abstract":"<div><div>The 2022 Mpox outbreak in the DRC highlighted the urgent need for rapid, fielddeployable diagnostic tools in resource-limited LMIC settings. A mobile diagnostic setup was evaluated, demonstrating operational readiness within 20 minutes and safe testing within 50 minutes. This approach proved practical for Mpox detection in remote, rural regions.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 3","pages":"Article 116800"},"PeriodicalIF":2.1,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fever of unknown origin: Clinical significance of the etiology and common inflammatory parameters","authors":"Nana Xie ,&nbsp;Wencong Zhang ,&nbsp;Fangbing Tian ,&nbsp;Wenjin Sun ,&nbsp;Mingyou Xing ,&nbsp;Qiurong Ruan ,&nbsp;Jianxin Song","doi":"10.1016/j.diagmicrobio.2025.116801","DOIUrl":"10.1016/j.diagmicrobio.2025.116801","url":null,"abstract":"<div><h3>Objectives</h3><div>To analyze the fever of unknown origin (FUO) etiology and clinical characteristics and determine the diagnostic value of common inflammatory parameters.</div></div><div><h3>Methods</h3><div>A total of 853 patients with classic FUO were prospectively enrolled from January 2014 to May 2021. According to the etiological diagnosis, the patients were divided according to etiology: infectious diseases (n = 430); non-infectious inflammatory diseases [NIIDs] (n = 153); neoplastic diseases (n = 125); miscellaneous diseases (n = 48); and no identifiable etiology (n = 97). The clinical features and common inflammatory parameters were analyzed.</div></div><div><h3>Results</h3><div>The clinical characteristics and common inflammatory parameters varied among the FUO patients with different etiologies. The tuberculosis T lymphocyte spot test (T-SPOT.TB) and C-reactive protein (CRP) level might be used as indicators to diagnose infections in patients with FUO. Female gender, young age, high white blood cell (WBC) count and percentage of neutrophil percent (NP), high erythrocyte sedimentation rate (ESR), and elevated serum ferritin (SF) level contributed to the diagnosis of NIIDs. Male gender, old age, low hemoglobin (HB) concentration and platelet (PLT) count, and high lactate dehydrogenase (LDH) and SF levels contributed to the diagnosis of neoplastic diseases. Male gender, old age, and low LDH and SF levels contributed to the diagnosis of infectious diseases. The procalcitonin (PCT) and interleukin-6 (IL-6) levels were highest in FUO patients with neoplastic diseases.</div></div><div><h3>Conclusions</h3><div>Infectious diseases are the most frequent cause of FUO, followed by NIIDs. The clinical characteristics and common inflammatory markers have significant value in determining the etiology of FUO.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 3","pages":"Article 116801"},"PeriodicalIF":2.1,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143686301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Performance of microscopy and rapid test for the diagnosis of mixed malaria in an endemic area of Colombia.","authors":"Alexandra Rios-Orrego,&nbsp;Tatiana M. Lopera-Mesa,&nbsp;Veronica Sierra Cifuentes,&nbsp;Lina Zuluaga-Idárraga","doi":"10.1016/j.diagmicrobio.2025.116799","DOIUrl":"10.1016/j.diagmicrobio.2025.116799","url":null,"abstract":"<div><div>Background: Mixed infection by <em>Plasmodium</em> (MIP) occurs when more than one species are infecting one individual at the same time. Methods: Frequency of MIP was determined in a cohort of patients with acute febrile syndrome. Diagnostic performance of microscopy (MI) and a rapid diagnostic test (RDT) was evaluated against real-time PCR (qPCR), as reference method. Results: The frequency of MIP was 3% by qPCR, 1.4% by MI and 0.4% by RDT. The sensitivity of MI and RDT for detecting MIP was 21.43% and 15.25%, respectively, and specificity was over 99% for both techniques. Conclusion: The currently available tests for the diagnosis of <em>Plasmodium</em> in the field, showed poor performance for detecting MIP in this study. It is necessary to improve diagnostic tests sensitivity for the correct detection of these coinfections and to monitor the elimination of parasitemia to detect possible recurrences due to inadequate treatments.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 3","pages":"Article 116799"},"PeriodicalIF":2.1,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bloodstream infections by multidrug-resistant Pluralibacter gergoviae in premature neonates in Central-West Brazil: Report of two cases","authors":"Caroline Tieppo Flores de Oliveira ,&nbsp;Bruna Abdul Ahad Saad ,&nbsp;Mara Luci Gonçalves Galiz Lacerda ,&nbsp;Bianca Stavis Conte ,&nbsp;Sandra Maria do Valle Leone de Oliveira ,&nbsp;James Venturini ,&nbsp;Anamaria Mello Miranda Paniago ,&nbsp;Rebeca Vitória da Silva Lage de Miranda ,&nbsp;Talita Bernardo Valadão ,&nbsp;Luciana Veloso da Costa ,&nbsp;Gabriel Vitor Dias Souza ,&nbsp;Debora Ribeiro de Souza Santos ,&nbsp;Stephen James Forsythe ,&nbsp;Cláudia Elizabeth Volpe-Chaves ,&nbsp;Marcelo Luiz Lima Brandão","doi":"10.1016/j.diagmicrobio.2025.116798","DOIUrl":"10.1016/j.diagmicrobio.2025.116798","url":null,"abstract":"<div><div>Two cases of bloodstream infections caused by multidrug-resistant <em>Pluralibacter gergoviae</em> (KPC+) in premature neonates hospitalized in a neonatal intensive care unit in Brazil are presented. Strains from these cases were confirmed by 16S rRNA sequencing and matrix assisted laser desorption ionization-time of flight/mass spectrometry using MALDI Biotyper®, with further typing using Fourier-transform infrared spectroscopy using IR Biotyper®. The cases were unrelated, as evidenced by clustering analysis. Both neonates displayed risk factors including prolonged hospitalization, invasive device use, and prior antibiotic treatments. Case 1 exhibited clinical recovery following treatment with polymyxin B and amikacin, whereas Case 2 resulted in death despite multiple antibiotic regimens. Isolated strains showed resistance to carbapenems and harbored the bla_KPC, highlighting the critical need for targeted diagnostics and stringent infection control in neonatal intensive care units. These findings underscore the significance of monitoring emerging pathogens like <em>P. gergoviae</em>, which possess high multidrug resistance and limited therapeutic options.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 2","pages":"Article 116798"},"PeriodicalIF":2.1,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143629520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Achromobacter xylosoxidans: An uncommon scalp infection leading to alopecia and biofilm formation","authors":"Leo Wan ,&nbsp;Kelly Kimball ,&nbsp;Austin Cusick ,&nbsp;Frank Morocco","doi":"10.1016/j.diagmicrobio.2025.116797","DOIUrl":"10.1016/j.diagmicrobio.2025.116797","url":null,"abstract":"<div><div><em>Achromobacter xylosoxidans</em> is an emerging opportunistic pathogen causing respiratory and systemic infections, mainly in immunocompromised individuals. Cutaneous infections remain uncommon. We present a unique case of a 60-year-old immunocompetent female with a persistent, pruritic, and malodorous scalp infection for over a year, leading to alopecia and biofilm formation, complicating treatment by increasing antibiotic resistance. Despite lacking typical risk factors, wound culture identified <em>A. xylosoxidans</em> with susceptibility to trimethoprim-sulfamethoxazole, which led to successful treatment alongside surgical debridement. This case highlights the need for clinicians to consider <em>A. xylosoxidans</em> in differential diagnoses of unusual skin infections, especially when biofilm formation is evident, and underscores the importance of targeted antibiotic therapy due to this pathogen's multidrug resistance.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 2","pages":"Article 116797"},"PeriodicalIF":2.1,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143629521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-invasive diagnosis of pulmonary tuberculosis and predictive potential for treatment outcomes via miR-146a and miR-155 levels","authors":"Li-Huan Wang,&nbsp;Ming-Li Xu","doi":"10.1016/j.diagmicrobio.2025.116795","DOIUrl":"10.1016/j.diagmicrobio.2025.116795","url":null,"abstract":"<div><div>Use of microRNAs is a promising area for non-invasive diagnosis of pulmonary tuberculosis (TB). Herein, we aimed to investigate the diagnostic potential of miR-146a and miR-155 in pulmonary TB and their predictive potential for patient treatment outcomes. The study included 60 patients with active TB, 50 patients with latent TB, and 50 individuals without TB infection. The patients with active TB were given standard treatment regimens and followed up for treatment outcomes. The serum and sputum expression levels of miR-146a and miR-155 were quantified by qRT-PCR. The patients presenting active TB had highest relative expressions of miR-146a and miR-155 in the serum and sputum compared to patients presenting latent TB and healthy volunteers (p  &lt;  0.0001). When miR-146a and miR-155 levels in the serum and sputum as tools to diagnose TB, all values of area under the receiver operating characteristics curve (AUROC) were beyond 0.9. The patients with active TB and presenting unfavorable treatment outcomes were demonstrated with higher relative expressions of miR-146a and miR-155 in the serum and sputum than those presenting favorable treatment outcomes (<em>p</em> &lt; 0.0001). When these two miRNAs as tools to predict treatment outcomes, values of AUROC were beyond 0.85. The study unveils detections of miR-146a and miR-155 in the serum and sputum may be a promising non-invasive method to diagnose TB and further to predict treatment outcomes following anti-TB treatment.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 2","pages":"Article 116795"},"PeriodicalIF":2.1,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143609958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peptoniphilus vaginalis bacteremia in a patient with diabetic foot infection: First reported case and literature review","authors":"Liyan Mao ,&nbsp;Chao Xu ,&nbsp;Xueman Wang,&nbsp;Lu Gong,&nbsp;Sui Gao,&nbsp;Ziyong Sun,&nbsp;Zhongju Chen","doi":"10.1016/j.diagmicrobio.2025.116794","DOIUrl":"10.1016/j.diagmicrobio.2025.116794","url":null,"abstract":"<div><h3>Background</h3><div><em>Peptoniphilus vaginalis</em>, a Gram-positive anaerobic coccus, is a recently characterized species within the <em>Peptoniphilus</em> genus. No studies have detailed its features. This report documents the first case of <em>P. vaginalis</em> bloodstream infection in a patient with diabetic foot infection, with a review of existing literature.</div></div><div><h3>Case presentation</h3><div>A 55-year-old male presented with multiple skin ulcers on both lower extremities and hyperpyrexia. Blood cultures were conducted, and analysis via matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) revealed two distinct colony morphologies, namely <em>Peptoniphilus spp.</em> and <em>Staphylococcus epidermidis</em>. Discrepancies between MALDI-TOF MS results and biochemical identification prompted 16S rRNA sequencing, which confirmed <em>P. vaginalis.</em> The patient underwent surgical debridement and received intravenous antibiotics (levofloxacin, linezolid, and ornidazole), resulting in favorable recovery.</div></div><div><h3>Conclusions</h3><div>This is the first report of <em>P. vaginalis</em> bloodstream infection in a patient with diabetic foot infection. It was observed that conventional biochemical identification methods and MALDI-TOF MS technology may not reliably detect <em>P. vaginalis</em>. In the absence of more advanced molecular methods, <em>P. vaginalis</em> could potentially be misidentified as <em>Peptoniphilus harei</em>, even in the previously published literature. Catalase testing may serve as a pragmatic discriminator in resource-limited settings<em>,</em> which required further validation. This underscores the necessity for employing molecular biology techniques to ensure precise diagnosis and optimize treatment strategies.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 2","pages":"Article 116794"},"PeriodicalIF":2.1,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143609957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Burden of acute hepatitis E virus in South Asia: Insights from Global Burden of Disease study 2021","authors":"Prakasini Satapathy ,&nbsp;Shilpa Gaidhane ,&nbsp;Ashok Kumar Bishoyi ,&nbsp;Subbulakshmi Ganesan ,&nbsp;Kavita V ,&nbsp;Swati Mishra ,&nbsp;Manpreet Kaur ,&nbsp;Ganesh Bushi ,&nbsp;Muhammed Shabil ,&nbsp;Rukshar Syed ,&nbsp;Sonam Puri ,&nbsp;Sunil Kumar ,&nbsp;Sabah Ansar ,&nbsp;Sanjit Sah ,&nbsp;Diptismita Jena ,&nbsp;Quazi Syed Zahiruddin ,&nbsp;Khang Wen Goh","doi":"10.1016/j.diagmicrobio.2025.116767","DOIUrl":"10.1016/j.diagmicrobio.2025.116767","url":null,"abstract":"<div><h3>Background</h3><div>Despite global progress, South Asian countries account for a disproportionate burden of Acute hepatitis E (AHE). Understanding the burden of AHE in this region is crucial for targeted interventions.</div></div><div><h3>Methods</h3><div>We used Global Burden of Disease (GBD) 2021 data to assess the burden of AHE across eight South Asian countries from 1990 to 2021. Joinpoint regression was used to analyze temporal trends and Estimated Annual Percentage Change (EAPC) was calculated to quantify trends. The relationship between age-standardized disability-adjusted life years rate (ASDR) and socio-demographic index (SDI) was assessed using smoothing spline model and Spearman rank correlation. Rates are expressed per 100,000 population.</div></div><div><h3>Results</h3><div>Bangladesh had highest age-standardized prevalence rate (ASPR) [33.27 (95 % Uncertainty Interval: 27.64 to 39.95)] and age-standardized incidence rate (ASIR) [433.01 (359.61 to 519.76)], while India had highest ASDR [9.52 (4.33 to 18.42)]. Males had higher ASIR and ASPR than females in most South Asian countries, except Bhutan and India, and higher ASDR except in Nepal and Pakistan. Bhutan had the highest EAPC for both sexes in ASPR and ASIR, while India had the highest EAPC in ASDR, closely followed by Bhutan for both sexes. Age group 5-9 had the highest ASPR and ASIR whereas &lt;1-year age group had the highest ASDR. There was an inverse relationship between ASDR and SDI (<em>R</em> = -0.49, <em>p</em> &lt; 0.01).</div></div><div><h3>Conclusion</h3><div>South Asia bears a high burden of AHE, with variations across countries. Improvements in Water Sanitation and Hygiene (WASH) services are needed to achieve Sustainable Development Goals 3 and 6.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 3","pages":"Article 116767"},"PeriodicalIF":2.1,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143644916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}