Diagnostic microbiology and infectious disease最新文献

{"title":"Rapid diagnosis of tuberculosis using a three-dimensional nanofiber paper-based electrochemical sensor","authors":"Manman Du ,&nbsp;Junting Zhou ,&nbsp;Zhiwei Zhang ,&nbsp;Tianteng Hao ,&nbsp;Junfeng Han ,&nbsp;Jin Wei ,&nbsp;Xinwu Xie ,&nbsp;Can Wang","doi":"10.1016/j.diagmicrobio.2025.117210","DOIUrl":"10.1016/j.diagmicrobio.2025.117210","url":null,"abstract":"<div><div>Tuberculosis (TB) is the leading cause of death caused by a single infectious pathogen. Currently, a variety of conventional methods are widely used for detecting <em>Mycobacterium tuberculosis</em> (<em>Mtb</em>) or diagnosing TB, which have made significant contributions to controlling the TB epidemic. However, these existing TB diagnostic methods still face some difficulties and cannot provide rapid, low-cost, high-sensitivity, and high-accuracy diagnostic results simultaneously. Therefore, it is imperative to develop new TB diagnostic methods that are rapid, cost-effective, and sensitive through rigorous evaluation of potential biomarkers and new detection methods. In this study, a three-dimensional nanofiber (3D NFs) paper-based electrochemical sensor was prepared using electrospinning technology, and <em>Mtb</em> secretory protein Ag85B was utilized as a biomarker to achieve point-of-care detection of TB clinical samples. The 3D NFs paper-based electrochemical sensor can achieve a wide range of <em>Bacillus Calmette–Guérin</em> detection of 10¹-10⁸ CFU/mL with good specificity. When the clinical bronchoalveolar lavage fluid samples were diluted 10³ or 10⁴ times, the 3D NFs paper-based electrochemical sensor could detect TB clinical samples with a sensitivity of up to 92.9 % and a specificity of 60 % within 5 min. 3D NFs paper-based electrochemical sensor has the advantages of low cost, high sensitivity, and fast response speed, and can realize rapid preliminary screening of TB patients.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117210"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145676876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical characteristics and outcomes of nocardiosis: A retrospective analysis of 44 cases","authors":"Xiao-Xi Liang,&nbsp;Chang-Wei Wei","doi":"10.1016/j.diagmicrobio.2025.117205","DOIUrl":"10.1016/j.diagmicrobio.2025.117205","url":null,"abstract":"<div><h3>Objective</h3><div>The aim of this study was to characterize the clinical features of nocardiosis and to enhance understanding of its diagnostic and therapeutic challenges.</div></div><div><h3>Methods</h3><div>A retrospective review was conducted on the clinical records of 44 patients diagnosed with nocardiosis between January 2018 and December 2020. Demographic data, underlying conditions, clinical manifestations, laboratory and imaging findings, microbiological results, and treatment outcomes were analyzed.</div></div><div><h3>Results</h3><div>Of the 44 cases, pulmonary infection was identified in 28 cases, disseminated infection in 13 cases, and extrapulmonary single-organ infection in 3 cases. Comorbid conditions were present in 40 patients, most commonly bronchiectasis, diabetes mellitus, nephropathies, and connective tissue disorders. Thirteen patients had a history of long-term glucocorticoid or immunosuppressive therapy. The predominant symptoms included cough, sputum production, and fever, accompanied by elevated white blood cell counts, neutrophil percentages, C-reactive protein, and procalcitonin levels. Pulmonary imaging commonly revealed patchy, nodular, or mass-like opacities, bronchiectasis, and cavitary lesions. Weakly acid-fast staining yielded positive results in 32 cases. The isolated pathogens primarily included <em>Nocardia asteroides, Nocardia farcinica</em>, and <em>Nocardia cyriacigeorgica</em>. Clinical improvement was observed in 37 patients following treatment, while 7 died.</div></div><div><h3>Conclusion</h3><div>Nocardiosis predominantly affects the lungs, with approximately one-third of cases progressing to disseminated infection. This condition frequently affects patients with comorbidities, particularly those receiving glucocorticoids or immunosuppressants. Although clinical manifestations are nonspecific, weakly acid-fast staining demonstrates high diagnostic utility and plays a valuable role in early identification.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117205"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145647757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of Streptococcus agalactiae Colonisation in pregnant women using culture and a novel qPCR Kit","authors":"Bilge Mazlumoğlu ,&nbsp;Leyla Genç ,&nbsp;Şule Birol İnce ,&nbsp;Süleyman Pelit ,&nbsp;Elif Seren Tanrıverdi ,&nbsp;Elif Aktaş","doi":"10.1016/j.diagmicrobio.2025.117189","DOIUrl":"10.1016/j.diagmicrobio.2025.117189","url":null,"abstract":"<div><h3>Background</h3><div>Maternal colonisation with <em>Streptococcus agalactiae</em> (GBS) is the most important risk factor for early-onset sepsis in newborns. We aimed to determine the rate of GBS colonisation in pregnant women evaluate the concordance between culture and a novel commercial polymerase chain reaction (PCR) test for screening. We also aimed to investigate the presence of the hypervirulent ST-17 clone and the potential risk factors that may affect colonisation.</div></div><div><h3>Material and Methods</h3><div>A total of 365 rectovaginal samples from pregnant women ≥36 weeks were investigated. Lim Broth was used for culture. PCR was done by the Bio-Speedy GBS (Group B Streptococcus) qPCR kit. The presence of the hypervirulent ST-17 clone was evaluated by PCR. Potential risk factors were evaluated via a survey.</div></div><div><h3>Results</h3><div>GBS was detected in 12.3 % (95 % CI: 9.3–16.1) of pregnant women by culture, in 15.3 % (95 % CI: 11.9–19.5) by direct qPCR and in 19.5 % (95 % CI: 15.6–23.9) by enrichment qPCR. Cohen's Kappa analysis revealed an ‘almost perfect’ level of agreement between culture and direct qPCR (κ = 0.85; 95 % CI: 0.74–0.97). Discordant results were observed in 13 cases (12 qPCR positive / culture negative, and one qPCR-negative / culture-positive). All isolates were susceptible to penicillin, while resistance to erythromycin and clindamycin were 37.8 % (95 % CI 25.1–52.4) and 33.3 % (95 % CI 21.4–47.9), respectively. ST-17 clone constituted 7 % (95 % CI:1.9-17.0) of the positive samples. GBS colonisation showed an association with sexual activity that approached the statistical significance threshold, and a significant association with education level.</div></div><div><h3>Conclusion</h3><div>Using a novel PCR test, almost perfect agreement was found between qPCR and culture, while the detection of colonisation was higher by qPCR. This study highlights the diagnostic contribution of molecular methods in GBS screening, even providing results after the onset of labor. The high prevalence of GBS, along with the circulation of hypervirulent clones underscores the critical importance of routine screening in pregnant women.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117189"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145555354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of hepatitis b virus infection among patients in the laboratory of jazeera specialist hospital, Mogadishu, Somalia, between 2020 - 2024","authors":"Mohamed Hussein Osman ,&nbsp;Qinghua Wang","doi":"10.1016/j.diagmicrobio.2025.117211","DOIUrl":"10.1016/j.diagmicrobio.2025.117211","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aimed to evaluate the prevalence of Hepatitis B Virus (HBV) infection among patients attending Jazeera Specialist Hospital in Mogadishu, Somalia, between January 2022 and December 2024.</div></div><div><h3>Patients and Methods</h3><div>A retrospective cross-sectional study was conducted using laboratory records of 18,745 patients tested for HBsAg. Descriptive statistics and Pearson Chi-Square tests were employed to evaluate prevalence patterns and infer associations between HBV status and variables such as gender, age, marital status, district of residence, and year of testing.</div></div><div><h3>Results</h3><div>The overall HBV prevalence was 3.1 % (<em>n</em> = 576), classifying the region as being under intermediate endemicity. Males exhibited a higher positivity rate (4.4 %) compared to females (2.5 %) (χ² = 51.665, <em>p</em> &lt; .001). Age-stratified analysis showed the highest infection rate among older adults aged 51–70 (6.5 %), followed by middle-aged adults aged 31–50 (5.3 %) (<em>χ²</em> = 250.314, <em>p</em> &lt; .001). Marital status was significantly associated with HBV positivity, with singles demonstrating a 3.6 % positivity versus 2.6 % among married individuals (<em>χ²</em> = 19.798, <em>p</em> &lt; .001). Notable geographic disparities were observed; districts such as Dharkeynley and Dayniile recorded higher positivity rates (5.4 % and 4.0 %, respectively) (<em>χ²</em> = 95.462, <em>p</em> = .001). Annual positivity varied, peaking in 2020 (3.9 %) and remaining statistically significant across years (<em>χ²</em> = 22.923, <em>p</em> = .028).</div></div><div><h3>Conclusions</h3><div>HBV remains a substantial health concern in Mogadishu, particularly among males, older adults, and residents of specific districts. The findings emphasise the urgent need for demographically targeted HBV prevention strategies, including vaccination, public health education, and enhanced district-level screening initiatives.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117211"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization of non-polio enterovirus in children with acute flaccid paralysis in Iraq","authors":"Mays Jassim Abdalkareem ,&nbsp;Hula younis fadhil ,&nbsp;Noor A. Hanoon","doi":"10.1016/j.diagmicrobio.2025.117169","DOIUrl":"10.1016/j.diagmicrobio.2025.117169","url":null,"abstract":"<div><h3>Background</h3><div>the association of Non-polio Enteroviruses (NPEVs) with acute flaccid paralysis (AFP) is growing anxiety worldwide, particularly, In Iraq.</div></div><div><h3>Objectives</h3><div>This study aimed to highlight the importance of the molecular techniques in the detection of the NPEVs and to understand if there are any effects of the capsid genes polymorphisms on the virus' behavior in the tissue culture.</div></div><div><h3>Study design</h3><div>During 2014–2016, from 799 stool samples of AFP' patients, 90 NPEVs cell culture positive and negative isolates have been collected on the basis on their cytopathic effect levels in the cell culture for detection by the reverse transcriptase polymerase chain reaction (RT-PCR). For further molecular study, the Viral Protien-4 capsid gene (VP4) of 17 NPEVs RT-PCR positive isolates and the evolutionary relationship of these isolates were analyzed by the nucleotide sequence analysis technique and the phylogenetic tree, respectively.</div></div><div><h3>Results</h3><div>the prevalence of the NPEVs positive results have been increased by the RT- PCR and the nucleotide sequence analyzed isolates were characterized as members of Enterovirus B with a predominance to the echoviruses, including echovirus (E)-4, E-7, E-9, E-14 and E-20. As well as, Coxsackie B virus, Enterovirus- 74, Enterovirus B-106 and untyped Enterovirus B strain. Noticeably, the E-4 and EV- 74 strains have been belonged for the recorded two death cases in this study. While an obvious coordination have been demonstrated between the evolutionary relationships of these isolates and their behavior in the tissue culture, the death case associated E-4 strain showed a different grouping pattern.</div></div><div><h3>Conclusion</h3><div>the VP4 gene sequencing, well typed the local strains into Enteroviruses B group. For best results, it's recommended to use other viral protein- capsids in the characterization of the NPEVs isolates.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117169"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145660526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unmasking the unexpected: Neuroinvasive listeriosis complicating influenza pneumonia","authors":"Riff Jean-Clément ,&nbsp;Desroches Marine ,&nbsp;Schmitt Mathilde ,&nbsp;Bernard Christine ,&nbsp;Bouvet Geoffroy ,&nbsp;Laurent Matthieu ,&nbsp;Baudic Thibaut ,&nbsp;Schaal Jean-Vivien","doi":"10.1016/j.diagmicrobio.2025.117214","DOIUrl":"10.1016/j.diagmicrobio.2025.117214","url":null,"abstract":"<div><div>We report the case of an 85-year-old patient hospitalized for influenza-associated pneumonia who developed a sudden febrile coma. This unexpected neurological deterioration in a presumed viral illness created a complex diagnostic challenge. Given the patient’s age and comorbidities, a bacterial co-infection was considered. Cerebrospinal fluid analysis and multiplex PCR enabled the rapid identification of <em>Listeria monocytogenes</em>, confirming listeria meningoencephalitis and allowing timely antimicrobial therapy. The patient had fully recovered at hospital discharge.</div><div>To our knowledge, the association between influenza and neurolisteriosis has not been previously reported. This case highlights the difficulty of evaluating febrile coma in older adults, particularly when symptoms are attributed to influenza. It underscores the importance of a broad diagnostic approach, even with confirmed influenza. Rapid molecular testing was essential in identifying a treatable central nervous system infection and improving management and prognosis in this high-risk population.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117214"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145767594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular diagnostics in clinical microbiology: Advances, applications, and future directions","authors":"Sijo Asokan ,&nbsp;Avinash Choudekar ,&nbsp;Arunkumar Jagadeesan ,&nbsp;Rudresh SM ,&nbsp;Aamir Ali ,&nbsp;Santosh Uttamrao Napte ,&nbsp;Saranya Ashokapuram Selvam ,&nbsp;Gaurav Verma ,&nbsp;Priti Das ,&nbsp;Nisha Beniwal ,&nbsp;Guhanraj Radhamanalan ,&nbsp;Smitha Vijayan ,&nbsp;Divya Rajeswary ,&nbsp;Teena Jacob","doi":"10.1016/j.diagmicrobio.2025.117223","DOIUrl":"10.1016/j.diagmicrobio.2025.117223","url":null,"abstract":"<div><div>Molecular diagnostics have transformed clinical microbiology by enabling rapid, accurate, and highly sensitive detection of infectious agents, significantly improving patient outcomes and public health response. Over the past decade, advances in polymerase chain reaction (PCR), next-generation sequencing (NGS), digital PCR, isothermal amplification, and CRISPR-based assays have enhanced pathogen identification, antimicrobial resistance profiling, and outbreak investigation. These technologies have been instrumental in detecting and monitoring emerging and re-emerging viral pathogens such as SARS-CoV-2, Nipah, Zika, H5N1 avian influenza, Mpox, Ebola, Marburg, and MERS-CoV. Molecular diagnostics also play a critical role in antimicrobial resistance surveillance and hospital infection control by enabling high-resolution tracking of resistance genes and pathogen transmission dynamics. Despite these achievements, challenges remain regarding implementation costs, technical expertise, infrastructure, and the need for global standardization. Future directions focus on developing cost-effective, point-of-care molecular platforms, integrating artificial intelligence and bioinformatics for enhanced interpretation, and applying these technologies within One Health and environmental surveillance frameworks. These innovations will be pivotal for early outbreak detection, real-time data-driven decision-making, and equitable access to advanced diagnostics worldwide. Ultimately, molecular diagnostics are poised to remain the cornerstone of precision medicine and infectious disease control in the era of global health challenges.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117223"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145773293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of human papillomavirus detection in tissue and urinary/plasmatic exosomes in prostatic adenocarcinoma: Links with clinicopathological parameters","authors":"Khaoula Elghazali ,&nbsp;Zineb Sakhi ,&nbsp;Youssef Ennaji ,&nbsp;Abdelilah Laraqui ,&nbsp;Anouar Elghazzaly ,&nbsp;Abdessamad Amine ,&nbsp;Moulay Mustapha Ennaji","doi":"10.1016/j.diagmicrobio.2025.117249","DOIUrl":"10.1016/j.diagmicrobio.2025.117249","url":null,"abstract":"<div><div>The etiological role of human papillomavirus (HPV) in prostate carcinogenesis remains controversial, despite its well-established causal association with cervical and oropharyngeal cancers. Exosomes, recognized as key mediators of intercellular communication, are increasingly implicated in viral dissemination and tumor progression, and have been proposed as minimally invasive diagnostic tools.</div><div>In this study, we investigated the prevalence of HPV DNA in prostate tissue, plasma-derived exosomes, and urine-derived exosomes, and examined its correlation with clinicopathological parameters in patients with histologically confirmed prostate cancer. Fifty patients were prospectively included. HPV DNA was detected in 32% of prostate tissue samples, 24% of plasma-derived exosomes, and 28% of urine-derived exosomes. Statistically significant associations were observed between HPV positivity and higher Gleason scores (<em>p</em> = 0.017), advanced T stage (<em>p</em> = 0.003), and alcohol consumption (<em>p</em> = 0.035). In contrast, only 2% of control samples were HPV DNA positive.</div><div>Notably, detection of HPV DNA in exosomal fractions mirrored tissue-based positivity, highlighting the potential of exosome-based liquid biopsies as noninvasive diagnostic approaches. While our findings support a possible association between HPV and prostate cancer, causality remains unresolved. Nevertheless, the study underscores the promise of exosome-based detection methods for future applications in noninvasive diagnosis, disease monitoring, and development of targeted therapeutic strategies—approaches that are already clinically relevant in HPV-driven cancers such as cervical cancer.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117249"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145880406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond soil and pets: Nannizzia gypsea causing kerion in a child","authors":"Sonali Padhy ,&nbsp;Malay Prithwiraj Sahoo ,&nbsp;Arpita Nibedita Rout ,&nbsp;Diptanu Paul ,&nbsp;Madhucchanda Das ,&nbsp;Vinaykumar Hallur","doi":"10.1016/j.diagmicrobio.2025.117243","DOIUrl":"10.1016/j.diagmicrobio.2025.117243","url":null,"abstract":"<div><div><em>Nannizzia gypsea</em> (formerly <em>Microsporum gypseum</em>) is a geophilic dermatophyte that rarely causes human infection. We report an 8-year-old boy presenting with a tender, edematous scalp lesion with pustules and alopecia for six weeks. Initially misdiagnosed as a bacterial furuncle, mycological examination of plucked hairs revealed hyaline septate hyphae, and culture on Sabouraud’s agar showed powdery buff colonies with brown reverse pigmentation. Lactophenol cotton blue mount demonstrated fusiform, thin-walled macroconidia with 4–6 septa. Molecular identification (ITS sequencing) confirmed <em>Nannizzia gypsea</em> with 100% homology. The patient was treated with oral terbinafine for four weeks, resulting in complete resolution without recurrence. This case is notable for the absence of soil or pet exposure, highlighting the diagnostic challenges of kerion due to geophilic species. Early mycological confirmation enables targeted therapy, prevents mismanagement, and supports antifungal stewardship.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117243"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145880492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular insights into viral agents of acute gastroenteritis detected among under five children in Assam, India","authors":"Gitika Rajbongshi ,&nbsp;Ajanta Sharma","doi":"10.1016/j.diagmicrobio.2025.117235","DOIUrl":"10.1016/j.diagmicrobio.2025.117235","url":null,"abstract":"<div><div>Acute gastroenteritis (AGE) is a significant cause of morbidity and mortality among children under five years where viral agents are major contributors to AGE. Limited data exist on molecular epidemiology of viral gastroenteritis in northeastern India. The present study aimed to determine the prevalence and molecular diversity of major viral agents causing AGE in children under five years in Assam, India. A hospital-based prospective study was conducted from January 2022 to December 2023. Stool samples (<em>n</em> = 110) from children with AGE were screened for rotavirus A (RVA), adenovirus (HAdV), norovirus (NoV), astrovirus (HAstV) and sapovirus (SaV) using multiplex real-time Polymerase Chain Reaction, followed by genotyping and sequencing of selected isolates. Phylogenetic analysis was conducted to identify specific circulating strains. Enteric viruses were detected in 44.5 % (49/110) of samples. HAdV (16.36 %) was the most prevalent, followed by RVA (11.81 %), NoV GII (7.27 %), SaV (0.9 %) and HAstV (0.9 %). Mixed infections occurred in 14.55 %, mainly HAdV–NoV GII. Phylogenetic analysis identified HAdV species F (serotypes 40 and 41), NoV genotypes GII.4 and GII.2, RVA genotype G3P[8] and HAstV-1. HAdV-41 was the predominant adenovirus type, closely related to global strains. No correlation was found between mixed infections and disease severity. The predominance of HAdV-41 and exclusive detection of RVA G3P[8] reflect evolving viral epidemiology in the post-rotavirus vaccine era. Continuous molecular surveillance and strengthened WASH (water, sanitation and hygiene) interventions are essential to guide vaccine strategies and reduce the burden of viral gastroenteritis in young children in northeast India.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117235"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145880494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}