Diagnostic microbiology and infectious disease最新文献

{"title":"Candida kefyr as an emerging cause of invasive fungal infection in transplant patients: Case report and literature review","authors":"Yasemin Nadir","doi":"10.1016/j.diagmicrobio.2025.117052","DOIUrl":"10.1016/j.diagmicrobio.2025.117052","url":null,"abstract":"<div><h3>Background</h3><div><em>Kluyveromyces marxianus</em>, formerly known as <em>Candida kefyr</em>, is an emerging opportunistic yeast increasingly reported in immunocompromised hosts. This review aims to summarize the clinical presentations, diagnostic approaches, treatment strategies, and outcomes of <em>C. kefyr</em> infections among transplantation recipients through an analysis of published case reports. A comprehensive literature search was performed using PubMed, Scopus, and Web of Science for case reports involving transplantation patients infected with <em>C. kefyr</em>. The review included only individual case reports describing invasive fungal infections in solid organ or hematopoietic stem cell transplant recipients. A total of seven case reports were identified. Clinical presentations included fungemia, keratitis and catheter-related bloodstream infections. Median age was 61.0. Most of the case had neutropenia and consumption of dairy products. Also, the ratio of localized organ involvement is higher. C. kefyr should be considered a potential opportunistic pathogen in transplant recipients, particularly in older or neutropenic patients with regular dairy consumption. Early diagnosis and appropriate antifungal therapy are essential for favorable outcomes.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"113 4","pages":"Article 117052"},"PeriodicalIF":1.8,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144831498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Time to positivity for differentiating blood culture contamination: A 20-hour cutoff for major contaminants","authors":"Yohei Manabe ,&nbsp;Hideharu Hagiya ,&nbsp;Shinnosuke Fukushima ,&nbsp;Kenta Nakamoto ,&nbsp;Kohei Oguni ,&nbsp;Hidemasa Akazawa ,&nbsp;Yasushi Fujita ,&nbsp;Takashi Kiguchi ,&nbsp;Koji Iio","doi":"10.1016/j.diagmicrobio.2025.117030","DOIUrl":"10.1016/j.diagmicrobio.2025.117030","url":null,"abstract":"<div><h3>Background</h3><div>Blood culture remains the gold standard for diagnosing bacteremia; however, contamination inevitably occurs in 2-3% of cases, requiring differentiation between true bacteremia and contamination. Although time to positivity (TTP) aids in this clinical decision, with detection after 24 hours generally indicating contamination, technological advances in blood culture systems may have shortened this threshold interval.</div></div><div><h3>Methods</h3><div>This study retrospectively analyzed blood culture data in our hospital from April 2023 to January 2025 to determine the optimal TTP cutoff. Patients with positive blood cultures for major contaminating bacteria were included. Cases were classified as true bacteremia or contamination based on a comprehensive chart review conducted by the antimicrobial stewardship audit, and TTP was compared between the groups. Sensitivity, specificity, and Youden index at various TTP cutoffs were utilized to determine the optimal threshold using the receiver operating characteristic curve analysis.</div></div><div><h3>Results</h3><div>Seventy-one patients were enrolled, with 34 cases classified as true bacteremia and 37 as contamination. Identified bacteria included coagulase-negative staphylococci (70.4%), viridans group streptococci (18.3%), and others (11.3%). The median TTP was significantly shorter in the true bacteremia group compared with the contamination group (18.6 vs.25.8 hours, <em>p</em> &lt; 0.001). In the contamination group, 43.2% of the cases demonstrated positive growth within 24 hours. Based on sensitivity, specificity, and Youden index, the optimal threshold was estimated to be 20 hours. A subgroup analysis of the CNS-only cohort yielded concordant results.</div></div><div><h3>Conclusion</h3><div>This study suggests that a 20-hour TTP threshold could help effectively differentiate true bacteremia from contamination in current clinical settings.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"113 4","pages":"Article 117030"},"PeriodicalIF":1.8,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144780539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of molecular and serological diagnostics for dengue during the 2023 outbreak in Jember, East Java, indonesia","authors":"Luthfiana Mutiara Sani ,&nbsp;Kartika Senjarini ,&nbsp;Salome Steinke ,&nbsp;Dao Thi Huyen ,&nbsp;Tran Thi Thu Hien ,&nbsp;Rike Oktarianti ,&nbsp;Tran Thi Thanh Huyen ,&nbsp;Le Huu Song ,&nbsp;Thirumalaisamy P. Velavan ,&nbsp;Do Duc Anh ,&nbsp;Truong Nhat My","doi":"10.1016/j.diagmicrobio.2025.117050","DOIUrl":"10.1016/j.diagmicrobio.2025.117050","url":null,"abstract":"<div><h3>Background</h3><div>Dengue virus (DENV) is a major public health concern in Indonesia, with all four serotypes contributing to recurrent outbreaks. This study evaluated molecular diagnostic tests for DENV and identified circulating serotypes despite limited national surveillance.</div></div><div><h3>Methods</h3><div>Clinically diagnosed dengue patients were recruited during the 2023 outbreak in Jember, Indonesia. Dengue was confirmed using Rapid Diagnostic Tests (RDTs) for NS1, IgM, IgG, and RT-PCR assays (Fast Track Diagnostics (FTD) and DENV in-house RT-qPCR), with diagnostic accuracy assessed using a composite reference standard. DENV serotypes were identified using Altona, CDC, and Serotype in-house RT-PCR.</div></div><div><h3>Results</h3><div>The FTD RT-PCR test showed higher sensitivity (100 %) than the DENV in-house test (78 %). All RDTs-positive cases were confirmed as DENV RNA-positive by RT-PCR. Serotyping detected all four DENV serotypes, with DENV-3/4 co-infections in seven patients. While the CDC and Serotype in-house tests effectively identified DENV-2, DENV-4, and DENV-3/4; DENV-1 was better detected by the Altona and Serotype in-house tests. The Altona test failed to detect DENV-4 in our study.</div></div><div><h3>Conclusions</h3><div>All four DENV serotypes were detected during the 2023 outbreak, with possible re-emergence of DENV-4. The FTD dengue test proved effective for confirming infections, though rapid serological tests remain a practical but less precise alternative.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"113 4","pages":"Article 117050"},"PeriodicalIF":1.8,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144780538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of antimicrobial therapy on bacterial burden in endotracheal aspirates from mechanically ventilated critical care patients with severe lower respiratory tract infection as assessed by the BIOFIRE® Filmarray® Pneumonia plus panel","authors":"Sofía Cano ,&nbsp;María Ángeles Clari ,&nbsp;David Bolado ,&nbsp;Nieves Carbonell ,&nbsp;David Navarro","doi":"10.1016/j.diagmicrobio.2025.117029","DOIUrl":"10.1016/j.diagmicrobio.2025.117029","url":null,"abstract":"<div><div>We investigated how antimicrobial therapy impacts bacterial loads in endotracheal aspirates from critical care patients with severe lower respiratory tract (LRT) infection as assessed by the Filmarray® pneumonia plus panel (FA-PP). This single-center, retrospective, observational study included 38 non-consecutive adult Intensive Care Unit patients undergoing invasive mechanical ventilation (IMV) with an FA-PP test returning detectable results for <em>Pseudomonas aeruginosa</em>, Enterobacterales, or Gram-positive microorganisms. Follow-up samples were collected within 12 days of the first one. <em>P. aeruginosa</em> was detected in nine patients. The <em>P. aeruginosa</em> load decreased in seven of these patients. All nine patients had been treated appropriately between sampling times. A total of 22 patients with Enterobacterales were included, representing 25 bacterial targets. Bacterial loads decreased for 15 targets in 12 patients, but either increased or remained unchanged for 10 targets in 10 patients. All but one patient were treated appropriately. A total of 19 patients had Gram-positive bacteria (<em>n</em> = 20) detected by the FA-PP, including <em>S. pneumoniae</em> (<em>n</em> = 10), <em>S. aureus</em> (<em>n</em> = 9), and <em>S. pyogenes</em> (<em>n</em> = 1). The administration of appropriate therapy (all patients) resulted in a decrease in bacterial burden for 14 targets in 14 patients. Bacterial loads remained unchanged or increased in for 6 targets in 6 patients. In conclusion the administration of appropriate antimicrobial therapy frequently results in a sizeable decrease in bacterial loads, as quantified by the FA-PP. This assay may prove useful for the assessment of the response to antibiotics in ICU patients with LRT infections undergoing invasive mechanical ventilation.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"113 4","pages":"Article 117029"},"PeriodicalIF":1.8,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144757798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Lemierre’s syndrome—A diagnostic challenge” [Diagnostic Microbiology and Infectious Disease 107 (2023) 116023]","authors":"Martin Bedan,&nbsp;Witold Gloksin,&nbsp;Trine Langfeldt Hagen","doi":"10.1016/j.diagmicrobio.2025.117022","DOIUrl":"10.1016/j.diagmicrobio.2025.117022","url":null,"abstract":"","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"113 4","pages":"Article 117022"},"PeriodicalIF":1.8,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144759455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silencing of ubiquitin specific protease-15 activates components of innate immune response and inhibits dengue virus infection","authors":"Athira AP ,&nbsp;Harikrishnan Jayakumar ,&nbsp;Aswathyraj S ,&nbsp;Shilpa Ravindran ,&nbsp;Ravi Prakash Arya ,&nbsp;Anismrita Lahon","doi":"10.1016/j.diagmicrobio.2025.117036","DOIUrl":"10.1016/j.diagmicrobio.2025.117036","url":null,"abstract":"<div><div>Innate immune responses induced by interferons are one of the key defense mechanisms against virus infections. It is activated by specific sensors in host cells that detect particular features of viruses. Secretion of interferons stimulate interferon-stimulated genes (ISGs) that act as antiviral effectors and inhibit virus infections. Many factors, including post-translational modifications (PTMs) of host cell proteins modulate virus infections. Ubiquitination/deubiquitination is one such PTM that utilizes ligases and deubiquitinases, which viruses exploit to enhance their replication and evade immune responses. Ubiquitin-specific proteases (USPs), a group of deubiquitinases, play a major role in virus transmission by modulating the replication and propagation of viruses. In this study, we examined the role of USP-15 during Dengue virus (DENV) infection. Our results showed that USP-15 plays a crucial role in facilitating DENV replication. Knockdown of USP-15 using specific siRNAs abrogated replication of DENV in A549 cells. This was evidenced by decreased expression of DENV-NS1 and envelope protein in A549 cells silenced with USP-15. Furthermore, higher expression of MDA-5, IFN-β, and ISG-15, sensors of innate immune receptors, was noted in USP-15-silenced DENV infected cells, indicating the association of antiviral genes with USP-15. Therefore, USP-15 could be utilized as an effective target for combating DENV infection.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"113 4","pages":"Article 117036"},"PeriodicalIF":1.8,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144763753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A fatal incidence of Yersinia enterocolitica meningitis. Case report","authors":"Alexander Dutschke ,&nbsp;Bo Langhoff Hønge ,&nbsp;Tine Birkelund Nielsen ,&nbsp;Susanne Schjørring ,&nbsp;Kristoffer Skaalum Hansen","doi":"10.1016/j.diagmicrobio.2025.116940","DOIUrl":"10.1016/j.diagmicrobio.2025.116940","url":null,"abstract":"<div><div><em>Yersinia enterocolitica</em> is commonly seen as a gastrointestinal infection and is an extremely rare cause of bacterial meningitis. We report a case of <em>Y. enterocolitica</em> meningitis in a man in his 90 s. The patient presented with seizures, a diffuse pupillary response and a fever. An emergency CT scan of the brain showed no abnormalities, and he was initially treated empirically for bacterial meningitis. The microbiological diagnosis was made by culture of cerebrospinal fluid. Although relevant antibiotics were quickly provided and infectious parameters improved, he never regained consciousness and passed away after 12 days of hospitalization. <em>Y. enterocolitica</em> was later cultured from a package of pork brawn from his refrigerator, making it the likely source of infection.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"113 3","pages":"Article 116940"},"PeriodicalIF":1.8,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144860694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Marked temporal decline in human herpesvirus-8 seroprevalence among people living with HIV in Istanbul, Türkiye","authors":"Yağmur Ekenoğlu Merdan ,&nbsp;Selim Merdan ,&nbsp;Pınar Etiz","doi":"10.1016/j.diagmicrobio.2025.117037","DOIUrl":"10.1016/j.diagmicrobio.2025.117037","url":null,"abstract":"<div><div>Human herpes virus 8 (HHV-8) drives Kaposi’s sarcoma and other lymphoproliferative disorders, especially in PLWH. There are differences in the prevalence of HHV-8 according to countries and geographical regions. Current knowledge on HHV-8 infection in Türkiye relies on sparse and outdated data, leaving a significant gap. To close this knowledge gap, we aimed to generate an up-to-date estimate of HHV-8 seroprevalence in PLWH in Istanbul, Türkiye.</div><div>In this study, a cross-sectional sero-survey of 368 PLWH whose blood samples were referred from six tertiary hospitals in Istanbul. HHV-8 IgG antibodies were detected with a commercial ELISA. Demographic data and HIV-RNA results accompanied each specimen. Categorical variables were compared with χ² or Fisher’s exact tests; continuous variables with independent-samples t-tests (<em>p</em> &lt; 0.05).</div><div>HHV-8 IgG antibodies were detected in 3 % of PLWH. No seropositive cases occurred in the 0–30-year age group, whereas rates peaked in the 51–60 (5.3 %) age group. Seropositivity was 4.2 % in females and 2.8 % in males (<em>p</em> &gt; 0.05). Among HIV-RNA–positive patients, HHV-8 positivity was 3.4 % versus 2.7 % in HIV-RNA–negative patients (<em>p</em> &gt; 0.05).</div><div>This multicenter representative study reveals that HHV-8 circulation among PLWH in Istanbul has declined to a low level. The absence of antibodies in younger adults suggests a recent reduction in saliva-mediated transmission, potentially linked to pandemic-driven measures. By providing the first robust sero-epidemiological estimate in over a decade, the study supplies an essential baseline for future public-health planning in Türkiye.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"113 4","pages":"Article 117037"},"PeriodicalIF":1.8,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144763754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validated cost-effective casein-based blocking buffers for enhanced neurocysticercosis ELISA accuracy","authors":"Daniela Darci Andriola ,&nbsp;Gabriela Nascimento Ferreira ,&nbsp;João Mânica Candelário ,&nbsp;Igor Negreiros Piazenski ,&nbsp;Marilene Biavatti ,&nbsp;Lucca Bonato Sakamoto ,&nbsp;João Carlos Minozzo ,&nbsp;Vanete Thomaz Soccol","doi":"10.1016/j.diagmicrobio.2025.117033","DOIUrl":"10.1016/j.diagmicrobio.2025.117033","url":null,"abstract":"<div><div>Accurate and affordable diagnostic tools are essential for managing neurocysticercosis<strong>,</strong> a parasitic infection of the central nervous system. In indirect ELISA, blocking buffers are crucial to prevent background noise and ensure reliable results, yet few studies have compared their performance in this specific context. This study assessed nine blocking solutions, four commercial and five prepared in-Lab, using an indirect ELISA with crude <em>Cysticercus cellulosae</em> antigen and a panel of 30 human serum samples (14 positive, 16 negative). By keeping all other variables constant, isolated the impact of each blocking buffer on test performance. Six formulations achieved perfect diagnostic accuracy (100 % sensitivity and specificity), while the remaining three ranged from 84.6 to 93.7 % sensitivity. ROC curve analysis confirmed high overall performance (AUC 0.957–1.000). Notably, a 3 % casein-based in-Lab blocker (B9) delivered the best results, offering strong analytical consistency, flawless diagnostic metrics, and a cost reduction of over 90 % compared to commercial alternatives. Despite the limited sample size, these findings demonstrate that well-prepared in-Lab blocking buffers, particularly those based on casein, can match or exceed commercial reagents in iELISA performance while drastically lowering costs. This approach has strong potential for expanding access to accurate NCC diagnostics in resource-limited settings.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"113 4","pages":"Article 117033"},"PeriodicalIF":1.8,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144757795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the NG-Test® CTX-M MULTI lateral flow immunoassay and genomic discovery of a blaCTX-M-27 variant with a premature stop codon","authors":"Geneviève Amaral ,&nbsp;Calvin Ka-Fung Lo ,&nbsp;Gordon Ritchie ,&nbsp;Jennifer Bilawka ,&nbsp;Christopher F. Lowe ,&nbsp;Marc G. Romney ,&nbsp;Willson Jang ,&nbsp;Leah Gowland ,&nbsp;Nancy Matic ,&nbsp;Michael Payne ,&nbsp;Linda Hoang ,&nbsp;Samuel D. Chorlton ,&nbsp;Aleksandra Stefanovic","doi":"10.1016/j.diagmicrobio.2025.117034","DOIUrl":"10.1016/j.diagmicrobio.2025.117034","url":null,"abstract":"<div><h3>Background</h3><div>Timely identification of extended-spectrum beta-lactamase (ESBL)-producing bacteria supports early antimicrobial optimization, especially for patients with invasive infections. NG-Test® CTX-M MULTI (CTX-M LFA) is a rapid immunochromatographic assay for detection of Cefotaximase-Munich (CTX-M)-type ESBL. We evaluated its performance against existing molecular platforms.</div></div><div><h3>Methods</h3><div>A sampling of archived Gram-negative bacteria (including <em>Enterobacterales</em> and non-fermenters) was included in the study. All isolates underwent testing for CTX-M ESBL by CTX-M LFA. CTX-M LFA results were compared to results from molecular methods, laboratory-developed (LD)-PCR (2013-2019) or BCID2 (2022-2023). Discordances were resolved with a combination of ESBL phenotypic testing and an alternative PCR platform, or whole-genome sequencing.</div></div><div><h3>Results</h3><div>Seventy-eight isolates were included in the study, all of which underwent CTX-M LFA and molecular testing (<em>n</em> = 47 by LD-PCR, <em>n</em> = 31 by BCID2). Post-discordance analysis showed positive percent agreement of 89.7 % (95 %CI 71.5-97.2 %), negative percent agreement 100 % (95 %CI 90.9-100 %) and overall categorical agreement 96.2 %, κ=0.92 (95 %CI 88.4-99.0 %). One <em>E. coli</em> was negative by CTX-M LFA but positive for <em>bla</em>_CTX-M by BCID2. Whole-genome sequencing identified a non-functional <em>bla</em>_CTX-M-27 gene with adenine insertion at position 70 in codon 24, causing a frameshift mutation resulting in a premature stop codon (codon 58).</div></div><div><h3>Conclusion</h3><div>CTX-M LFA improved performance in comparison with molecular methods by accurately identifying an isolate with a prematurely truncated protein as a true-negative for CTX-M. This highlights potential limitations of molecular-based resistance detection. CTX-M LFA was simple to perform and yielded results within 15 minutes.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"113 4","pages":"Article 117034"},"PeriodicalIF":1.8,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144757797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}