Diagnostic microbiology and infectious disease最新文献

Antifungal susceptibility of a collection of Aspergillus fumigatus strains isolated from patients with invasive pulmonary aspergillosis and COVID-19 associated aspergillosis 侵袭性肺曲霉病和COVID-19相关曲霉病患者分离的烟曲霉菌株的抗真菌敏感性

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-05-01 DOI: 10.1016/j.diagmicrobio.2025.116884

Andrea Liberatore , Giulia Lombardi , Donatella Lombardo , Tiziana Lazzarotto , Claudio Foschi , Simone Ambretti

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Refractory tenosynovitis caused by Mycobacterium virginiense required repeated debridement: A case report and literature review 由维吉尼亚分枝杆菌引起的难治性腱鞘炎需要反复清创：1例报告并文献复习

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-04-30 DOI: 10.1016/j.diagmicrobio.2025.116874

Takafumi Sekiguchi , Hitoshi Kawasuji , Mineyuki Zukawa , Nana Sugie , Kazushige Sugie , Mika Morita , Kentaro Nagaoka , Yoshitomo Morinaga , Hideki Niimi , Yoshihiro Yamamoto

{"title":"Refractory tenosynovitis caused by Mycobacterium virginiense required repeated debridement: A case report and literature review","authors":"Takafumi Sekiguchi , Hitoshi Kawasuji , Mineyuki Zukawa , Nana Sugie , Kazushige Sugie , Mika Morita , Kentaro Nagaoka , Yoshitomo Morinaga , Hideki Niimi , Yoshihiro Yamamoto","doi":"10.1016/j.diagmicrobio.2025.116874","DOIUrl":"10.1016/j.diagmicrobio.2025.116874","url":null,"abstract":"<div><div><em>Mycobacterium virginiense</em> is a slow-growing mycobacterium first described in 2016 as a novel species within the <em>Mycobacterium terrae</em> complex. To date, 8 cases of <em>M. virginiense</em> tenosynovitis or osteomyelitis have been reported, though none have been relapsed cases. A previously healthy 70-year-old Japanese man presented to a local orthopedic clinic with pain, swelling, and stiffness in his right middle finger. Initially diagnosed with trigger finger, he received multiple steroid injections. He was ultimately diagnosed with <em>M. virginiense</em> tenosynovitis. Despite synovectomy and antimicrobial therapy with ethambutol, rifampicin, and clarithromycin, a relapse occurred, necessitating a second tenosynovectomy, which led to remission. Antimicrobial therapy continued for approximately 20 months without recurrence. This case illustrates that <em>M. virginiense</em> can cause refractory tenosynovitis even in immunocompetent hosts. Delayed diagnosis and repeated local steroid injections may have contributed to disease progression and treatment failure in <em>M. virginiense</em> tenosynovitis.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"113 1","pages":"Article 116874"},"PeriodicalIF":2.1,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143903248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cover 2 - Aims/Scopes, Ed Board 封面2 -目标/范围，教育局

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-04-30 DOI: 10.1016/S0732-8893(25)00177-4

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S. suis serotype 2 meningitis in a patient with horse contact in a Japanese stable: A case report 日本马厩中与马接触的1例猪链球菌血清2型脑膜炎病例报告

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-04-25 DOI: 10.1016/j.diagmicrobio.2025.116868

Jura Oshida , Hiroshi Ito , Kimiko Ubukata , Misako Takata , Kiyofumi Ohkusu , Shunsuke Ohba , Akihiko Saida , Shigeki Nakamura , Daiki Kobayashi

引用次数: 0

A case of cutaneous leishmaniasis by a needlestick injury: an occupational infection or “experimental infection”? 针刺致皮肤利什曼病1例：职业性感染还是“实验性感染”？

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-04-24 DOI: 10.1016/j.diagmicrobio.2025.116863

Fabrício Marcus Silva Oliveira , Jacqueline Araujo Fiuza , Soraya T Gaze

{"title":"A case of cutaneous leishmaniasis by a needlestick injury: an occupational infection or “experimental infection”?","authors":"Fabrício Marcus Silva Oliveira , Jacqueline Araujo Fiuza , Soraya T Gaze","doi":"10.1016/j.diagmicrobio.2025.116863","DOIUrl":"10.1016/j.diagmicrobio.2025.116863","url":null,"abstract":"<div><div>Leishmaniasis is a Neglected Tropical Disease caused by unicellular parasitic kinetoplastids of the genus <em>Leishmania</em> spp., with 20 species associated with human infections. This case report describes an accidental inoculation of <em>Leishmania major</em> suffered by a graduate student in a research and education institution laboratory. Eight months after the occupational accident, the student developed an erythematous, painless papule at the needlestick injury site. Within a week, the lesion worsened, showing ulceration and an increase in size. The diagnosis of cutaneous leishmaniasis was based on clinical observations and confirmed by histopathological analysis, immunohistochemistry, and parasite culture. Following diagnosis, the patient was referred for treatment with a dosage of 81 mg SbV/kg of Glucantime, administered slowly via intravenous infusion diluted in 100 ml of 5 % glucose saline solution, every 24 h for 20 consecutive days. Furthermore, this case report emphasises the critical need for periodic training for research professionals to ensure their knowledge of protocols guiding prevention and response to occupational infections.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"113 1","pages":"Article 116863"},"PeriodicalIF":2.1,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143898628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oral fluid versus blood in HIV self-testing: A step towards “95-95-95” targets of the UNAIDS? 艾滋病毒自检中的口腔液与血液：向联合国艾滋病规划署“95-95-95”目标迈进了一步？

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-04-23 DOI: 10.1016/j.diagmicrobio.2025.116867

Pachamuthu Balakrishnan , Ramachandran Vignesh , Pitchaipillai Sankar Ganesh , Saravanan Shanmugam , Iyanar Kannan , Sathasivam Sivamalar , Sivadoss Raju , Vijayakumar Velu , Esaki M Shankar , Sree T. Sucharitha

{"title":"Oral fluid versus blood in HIV self-testing: A step towards “95-95-95” targets of the UNAIDS?","authors":"Pachamuthu Balakrishnan , Ramachandran Vignesh , Pitchaipillai Sankar Ganesh , Saravanan Shanmugam , Iyanar Kannan , Sathasivam Sivamalar , Sivadoss Raju , Vijayakumar Velu , Esaki M Shankar , Sree T. Sucharitha","doi":"10.1016/j.diagmicrobio.2025.116867","DOIUrl":"10.1016/j.diagmicrobio.2025.116867","url":null,"abstract":"<div><div>The population of HIV-infected individuals who are not aware of their HIV status or who do not usually have access to HIV services should be reached by the newer strategies. The WHO's guideline on HIV self-testing (HIV-ST) was based on a boost in the number of tests administered in randomized clinical trials, including those carried out among the general population in regions with a high HIV burden. Therefore, HIV-ST offers tremendous potential as a strategy to enhance testing frequency and improve access to HIV testing among key high-risk populations and their partners. Recent studies have documented that HIV-ST with oral fluid is perceived as a more convenient and also preferred option than using the finger prick blood-based testing due to “fear of prick”, and have also reported of user’s error with finger prick blood-based tests increasing than oral fluid-based test. The major benefit of oral fluid-based HIV-ST is that it makes it easier for first-time users to access testing and is more likely to increase testing frequency among key high-risk populations. The current review summarizes the pros and cons of the HIV-ST devices.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"113 1","pages":"Article 116867"},"PeriodicalIF":2.1,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143912110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The value of targeted next-generation sequencing in the diagnosis and differential diagnosis of paucibacillary pulmonary tuberculosis 靶向新一代测序在少杆菌性肺结核诊断和鉴别诊断中的价值

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-04-22 DOI: 10.1016/j.diagmicrobio.2025.116866

Hu Qianfang, Liu Yan, Chen Yuhan, Zhong Houyu, Wan Tao

{"title":"The value of targeted next-generation sequencing in the diagnosis and differential diagnosis of paucibacillary pulmonary tuberculosis","authors":"Hu Qianfang, Liu Yan, Chen Yuhan, Zhong Houyu, Wan Tao","doi":"10.1016/j.diagmicrobio.2025.116866","DOIUrl":"10.1016/j.diagmicrobio.2025.116866","url":null,"abstract":"<div><h3>Background</h3><div>The diagnostic utility of broad-spectrum pathogen-specific targeted next-generation sequencing (tNGS) for paucibacillary pulmonary tuberculosis (PTB) in general hospital settings remains insufficiently characterized compared to validated Mycobacterium-specific tNGS.</div></div><div><h3>Objective</h3><div>To investigate the clinical value of broad-spectrum pathogen-specific tNGS in diagnosing and differentially diagnosing paucibacillary PTB in real-world clinical practice.</div></div><div><h3>Methods</h3><div>This retrospective study analyzed data from 423 patients admitted to the First Affiliated Hospital of Chongqing Medical University from January 1, 2024, to October 31, 2024. Patients underwent bronchoalveolar lavage fluid (BALF) testing, including acid-fast staining, culture, Xpert MTB/RIF, and tNGS. 47 participants were excluded based on predefined criteria.</div></div><div><h3>Results</h3><div>Among 83 microbiologically confirmed PTB cases, tNGS exhibited significantly higher sensitivity compared to Xpert and culture (91.6 % vs. 79.5 % for Xpert and 73.5 % for culture; <em>p</em><0.001), albeit with marginally lower specificity than Xpert (95.6 % vs. 98.6 %; <em>p</em>=0.01). For 293 non-TB patients, tNGS detected pathogens in 86.7 % (254/293) of BAL samples, identifying 300 pathogens with 47.3 % (142/300) concordance to clinical diagnoses.</div></div><div><h3>Conclusion</h3><div>Broad-spectrum pathogen-specific tNGS demonstrated enhanced sensitivity for diagnosing paucibacillary PTB but slightly reduced specificity relative to Xpert. While it achieved high pathogen detection rates in non-TB pulmonary diseases, its concordance with clinical diagnoses was suboptimal.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"113 1","pages":"Article 116866"},"PeriodicalIF":2.1,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143887227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Performance of a molecular beacon-based qPCR method for detection of fluoroquinolone resistance in Mycobacterium tuberculosis: A study confirmed by sequencing 基于分子信标的qPCR检测结核分枝杆菌氟喹诺酮类药物耐药性的性能：经测序证实的研究

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-04-22 DOI: 10.1016/j.diagmicrobio.2025.116865

Oguz Arı , Rıza Durmaz , Ahmet Arslanturk

{"title":"Performance of a molecular beacon-based qPCR method for detection of fluoroquinolone resistance in Mycobacterium tuberculosis: A study confirmed by sequencing","authors":"Oguz Arı , Rıza Durmaz , Ahmet Arslanturk","doi":"10.1016/j.diagmicrobio.2025.116865","DOIUrl":"10.1016/j.diagmicrobio.2025.116865","url":null,"abstract":"<div><div>Multidrug-resistant tuberculosis (MDR-TB) poses a serious threat to the control and treatment of tuberculosis (TB). Rapid diagnosis of resistant strains via utilization of molecular techniques is of critical importance on the proper management of the patients. The objective of this study was to develop a quantitative polymerase chain reaction (qPCR) method for the detection of mutation-based fluoroquinolone (FQ) resistance in Mycobacterium tuberculosis isolates. Primers and a molecular-beacon probe were designed to target quinolone-resistance-determining-region (QRDR) of the <em>gyr</em>A gene of <em>M. tuberculosis</em>. Amplification conditions and concentrations of primers/probe were optimized, and the effectiveness of the optimized qPCR method was tested on 50 MDR <em>M. tuberculosis</em> strains. To confirm the qPCR results, all strains were also screened for mutations in the <em>gyr</em>A gene using Sanger sequencing. The optimized qPCR method had analytical sensitivity of 96.5 cfu/ml. The method detected FQ resistance in 7 (14 %) of the 50 MDR-strains via either no or significantly decreased fluorescence signal due to mutations associated with FQ resistance. The sequencing of these seven strains detected three resistance-associated mutations (A90V, D94A and D94G). Of the 43 FQ-susceptible strains, 10 strains with wild-type gene sequences yielded strong fluorescent signals above 450 RFU, while the remaining 33 strains harboring a non-resistance-associated mutation (S95T) showed decreased fluorescence signals <350 RFU. The sensitivity and specificity of the qPCR method for detection of the mutations related to resistance was ≥99.9 % and 98 %, respectively. Consequently, the utility of the optimized qPCR method for the identification of mutation-based FQ resistance is promising.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 4","pages":"Article 116865"},"PeriodicalIF":2.1,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143876946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A bibliometric and visual analysis of the research status and hotspots of Pulmonary Aspergillosis based on web of science 基于web of science的肺曲霉病研究现状与热点的文献计量与可视化分析

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-04-20 DOI: 10.1016/j.diagmicrobio.2025.116864

Jianye Li , Qingjun Zhu , Huixin Yan , Ting Ma , Yun An

{"title":"A bibliometric and visual analysis of the research status and hotspots of Pulmonary Aspergillosis based on web of science","authors":"Jianye Li , Qingjun Zhu , Huixin Yan , Ting Ma , Yun An","doi":"10.1016/j.diagmicrobio.2025.116864","DOIUrl":"10.1016/j.diagmicrobio.2025.116864","url":null,"abstract":"<div><h3>Background</h3><div>Pulmonary Aspergillosis (PA) is a common fungal lung infection. Despite recent advancements, bibliometric studies on PA are scarce. This study uses bibliometric methods to analyze current research trends and key topics, offering insights into future directions in the field.</div></div><div><h3>Methods</h3><div>PA-related literature was retrieved from the Web of Science Core Collection (WOSCC) database, and detailed analysis was conducted using CiteSpace, VOSviewer, and Excel 2019 software. This analysis aimed to identify trends and hot topics in the field of PA.</div></div><div><h3>Results</h3><div>A total of 1,715 articles were analyzed from 1900 to 2024. The number of publications has shown steady growth, with a gradual increase from 1990 to 2019, followed by a sharp rise after 2019. The United States leads in this field. The main research hotspots and frontiers in PA include: the close association between PA and immunocompromised conditions, COVID-19 as a new risk factor for PA, and current clinical research focusing on antifungal treatments and enhancing host immunity.</div></div><div><h3>Conclusion</h3><div>This study reveals trends in PA research, notably the sharp increase in publications post-2019. Novel findings include the identification of COVID-19 as a new risk factor for PA and the growing emphasis on antifungal treatments and host immunity enhancement. These insights provide a clearer direction for future clinical and research priorities in PA.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 4","pages":"Article 116864"},"PeriodicalIF":2.1,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143860345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in early respiratory tract microecology associated with neonatal respiratory distress syndrome 新生儿呼吸窘迫综合征与早期呼吸道微生态变化相关

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-04-17 DOI: 10.1016/j.diagmicrobio.2025.116848

Kun Du, Qian Wei, Jin Gao, Xing-Yu Xiong, Jia-Xin Li, Yuan Yuan

{"title":"Changes in early respiratory tract microecology associated with neonatal respiratory distress syndrome","authors":"Kun Du, Qian Wei, Jin Gao, Xing-Yu Xiong, Jia-Xin Li, Yuan Yuan","doi":"10.1016/j.diagmicrobio.2025.116848","DOIUrl":"10.1016/j.diagmicrobio.2025.116848","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to explore the distribution patterns of the early respiratory tract microbiome in infants with neonatal respiratory distress syndrome.</div></div><div><h3>Methods</h3><div>The study involved infants with neonatal respiratory distress syndrome who were admitted to the neonatal department of Kunming Children’s Hospital between July and October 2022. Samples were obtained at two time points: 1 to 3 days post-admission (initial group) and 7 to 10 days post-admission (later group). Pharyngeal swabs and lower respiratory tract sputum samples were analyzed using 16S ribosomal RNA gene sequencing to assess the composition and variations of the early airway microbiome.</div></div><div><h3>Results</h3><div>The predominant microbiota identified in both pharyngeal and lower respiratory tract sputum samples at 1 to 3 days and 7 to 10 days after birth included <em>Streptococcus, Enterobacter, Halomonas, Acinetobacter</em>, and <em>Serratia</em>, with the first three being the most dominant. The microbial distribution patterns were largely consistent between the initial and later groups in both sample types, exhibiting minimal age-related variation. Although the later group demonstrated higher microbial community richness and lower diversity compared to the initial group, these differences were not statistically significant. Notably, significant differences were observed in the richness index, ACE index, and Chao1 index of the pharyngeal microbiota between the initial and later groups.</div></div><div><h3>Conclusion</h3><div>The respiratory tract microbial composition in infants with neonatal respiratory distress syndrome remained relatively stable between 1 and 3 days and 7 to 10 days post-birth, although changes in the abundance and diversity of the respiratory tract microbiota were observed with advancing age.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 4","pages":"Article 116848"},"PeriodicalIF":2.1,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143882199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0