Diagnostic microbiology and infectious disease最新文献

{"title":"Analytical and clinical performance evaluation of the STANDARD™ M10 MTB/NTM assay for the simultaneous detection of Mycobacterium tuberculosis and nontuberculous mycobacteria.","authors":"Eun-Soon Son, Se Yeon Lee, Moon-Joo Lee, Sia Kang, Sunyoung Lee, Jong Seok Lee","doi":"10.1016/j.diagmicrobio.2026.117692","DOIUrl":"https://doi.org/10.1016/j.diagmicrobio.2026.117692","url":null,"abstract":"<p><p>The global surge in nontuberculous mycobacteria (NTM) infections, which clinically mimic Mycobacterium tuberculosis (MTB) infections, underscores the need for rapid differential diagnosis. This study evaluated the analytical and clinical performance of the STANDARD™ M10 MTB/NTM assay, a fully automated, cartridge-based, sample-to-result real-time PCR system. Analytical performance was assessed using contrived artificial sputum samples to evaluate inclusivity (3 MTB-complex, 12 NTM species), cross-reactivity (65 respiratory pathogens), and interference (33 substances). The limit of detection (LoD) was compared with two commercial assays, the AdvanSure™ TB/NTM Real-time PCR and the Anyplex™ MTB/NTMe Real-time Detection, using three MTB-complex strains (M. tuberculosis H37Rv, M. bovis, and M. bovis BCG Pasteur) and 15 NTM species. Clinical validation was performed using 70 clinical sputum specimens against both comparators. The STANDARD™ M10 MTB/NTM demonstrated excellent inclusivity and high specificity, with no cross-reactivity or analytical interference (△Ct ≤ 2.0). In the LoD comparison, the STANDARD™ M10 MTB/NTM achieved a lower LoD for MTB H37Rv (10¹ CFU/mL) and major NTM species (M. avium, M. intracellulare, M. abscessus subsp. abscessus) than the comparators, demonstrating superior analytical sensitivity. Moreover, the STANDARD™ M10 MTB/NTM successfully detected M. xenopi, M. scrofulaceum, and M. malmoense, which were missed by alternative assays. In clinical evaluation, it demonstrated 100% overall agreement (95% confidence interval (CI), 94.8-100.0) with the AdvanSure™ TB/NTM Real-time PCR and Anyplex™ MTB/NTMe Real-time Detection assays across a Ct range of 27.41-33.17. The STANDARD™ M10 MTB/NTM assay offers exceptional analytical sensitivity, broad coverage, and, in the absence of an independent reference standard, complete agreement with two established laboratory-based platforms. By incorporating a fully automated workflow and minimizing turnaround time, this system serves as a reliable tool for rapid MTB/NTM differentiation in point-of-care settings.</p>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"117 1","pages":"117692"},"PeriodicalIF":1.8,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149227567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disseminated MDR tuberculosis manifesting as Pott's disease with tuberculous septic ankle arthritis: an uncommon sequential dual-site involvement.","authors":"Jamil Muqtadir, Irshad Batool, Ayesha Akhtar, Sameeullah Bhatti","doi":"10.1016/j.diagmicrobio.2026.117685","DOIUrl":"https://doi.org/10.1016/j.diagmicrobio.2026.117685","url":null,"abstract":"<p><p>Disseminated osteoarticular TB is uncommon, and the occurrence of multidrug-resistant (MDR) strains impacting many skeletal locations in an immuno-competent individual is very rare. A 25-year-old male from a TB-endemic rural region is presented, having a three-month progression of worsening low back pain, subsequently accompanied by lower limb weakness. GeneXpert from both sites detected Mycobacterium tuberculosis that was resistant to rifampicin. The line probe assay detected resistance to rifampicin, high-level resistance to isoniazid, and resistance to ethambutol. After surgical procedures in the form of spinal decompression and arthrotomy, the patient had a 24-month all-oral bedaquiline-based treatment. Neurological recovery was noted during a period of 6 to 8 months, with the normalization of ankle function by 4-months. This case exemplifies a rare presentation of disseminated osteoarticular multidrug-resistant tuberculosis in an immunocompetent adult.</p>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"117 1","pages":"117685"},"PeriodicalIF":1.8,"publicationDate":"2026-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149223499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Congenital syphilis as a failure of the maternal-fetal prevention cascade: Clinical implications for pregnant migrant and structurally vulnerable women in high-income settings - an ESCMID study group for infections in Travellers and migrants (ESGITM) perspective.","authors":"A R Meduri, I A Ritacco, D Capruzzi, A Cuxart-Graell, R Buonamassa, F B Farkas, C Foti, G Ciccarese, B Lakatos, A Saracino, E Puca, K Aykac, A Vimercati, L Frallonardo, R Massmann, G Guido, E Milano, P Locantore, R Iatta, C Rönnberg, C Wejse, F Di Gennaro","doi":"10.1016/j.diagmicrobio.2026.117679","DOIUrl":"https://doi.org/10.1016/j.diagmicrobio.2026.117679","url":null,"abstract":"<p><strong>Background: </strong>Congenital syphilis remains a preventable but persistent cause of fetal loss, neonatal morbidity, and early-life complications. In high-income settings, its occurrence rarely reflects the absence of diagnostic tools or effective therapy; but more often exposes missed opportunities across antenatal care, sexually transmitted infection prevention, maternal treatment, partner management, and neonatal follow-up. This review investigates congenital syphilis from the viewpoint of the possible failures of the maternal-fetal prevention pathway, with a specific focus on pregnant migrant and structurally vulnerable women.</p><p><strong>Methods: </strong>This narrative clinical review synthesizes epidemiological data, international guidelines, surveillance reports, and selects peer-reviewed literature on maternal and congenital syphilis, antenatal screening, vertical transmission, penicillin treatment, neonatal assessment, migrant health, and structural barriers to care. Relevant sources available up to june 2026 have been identified through targeted searches of PubMed/MEDLINE, institutional websites, and guideline repositories, including those of WHO, CDC, ECDC, USPSTF, and BASHH.</p><p><strong>Results: </strong>Congenital syphilis may result from inadequacies at multiple points: delayed antenatal access, missed or late screening, incomplete or non-stage-appropriate treatment, untreated partners, reinfection, poor documentation, and inadequate neonatal follow-up. Pregnant migrant women and structurally vulnerable women may face additional barriers, including language difficulties, administrative insecurity, unstable housing, stigma, unsafe partner disclosure, mobility, and fragmented care across the various emergency centres, sexual health, obstetric, and neonatal services. Prevention should therefore prioritize reducing missed opportunities through early and repeated screening when indicated, same-visit testing when follow-up is uncertain, rapid linkage between antenatal and STI services, quantitative non-treponemal testing, penicillin-based stage-appropriate treatment, safe partner management, and clear maternal-neonatal documentation.</p><p><strong>Conclusions: </strong>Congenital syphilis should be considered a sentinel event revealing failings in antenatal care, STI prevention, migrant health access, and continuity of care. For pregnant migrant and structurally vulnerable women, prevention requires integrated, accessible, and safely coordinated maternal-neonatal pathways.</p>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"117 1","pages":"117679"},"PeriodicalIF":1.8,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149223517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Kernel density-encoded MALDI-TOF spectra with LightGBM machine learning for multiplex resistance prediction in Klebsiella pneumoniae\" [Diagnostic Microbiology & Infectious Disease 116 (2026) 117581].","authors":"Keqing Zhang, Heng Shen, Yanyan Hu, Zelin Yan, Linping Zhang, Rong Zhang","doi":"10.1016/j.diagmicrobio.2026.117666","DOIUrl":"https://doi.org/10.1016/j.diagmicrobio.2026.117666","url":null,"abstract":"","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":" ","pages":"117666"},"PeriodicalIF":1.8,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149059324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction notice to \"Rabies presenting as acute psychiatric behavioral abnormality with delayed onset after dog bite: A fatal case report\" [Diagnostic Microbiology & Infectious Disease 113 (2025) 116881].","authors":"Lan Mou, Hong Wang, Jianhua Li, Shikai Wang","doi":"10.1016/j.diagmicrobio.2026.117627","DOIUrl":"10.1016/j.diagmicrobio.2026.117627","url":null,"abstract":"","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":" ","pages":"117627"},"PeriodicalIF":1.8,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148911233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Development of a multiplex real-time RT-PCR assay for simultaneous detection and differentiation of influenza A, B, C, and D viruses\" [Diagnostic Microbiology and Infectious Disease 95 (2019) 59-66].","authors":"Hewei Zhang, Yin Wang, Elizabeth Porter, Nanyan Lu, Yanhua Li, Fangfeng Yuan, Molly Lohman, Lance Noll, Wanglong Zheng, Colin Stoy, Yuekun Lang, Victor C Huber, Wenjun Ma, Lalitha Peddireddi, Ying Fang, Jishu Shi, Gary Anderson, Xuming Liu, Jianfa Bai","doi":"10.1016/j.diagmicrobio.2026.117622","DOIUrl":"https://doi.org/10.1016/j.diagmicrobio.2026.117622","url":null,"abstract":"","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":" ","pages":"117622"},"PeriodicalIF":1.8,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148886721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic diversity of plasmodium vivax merozoite surface protein 3α and β field isolates","authors":"Nargis Shaheen ,&nbsp;Asma Ashraf ,&nbsp;Nida Malik ,&nbsp;Muneeba Naeem ,&nbsp;Ismail Zeb ,&nbsp;Ghulam Nabi ,&nbsp;Maher S. Alwethaynani ,&nbsp;Rania Ali El Hadi Mohamed ,&nbsp;Manal F. Elkhadragy ,&nbsp;Nawal Al-Hoshani","doi":"10.1016/j.diagmicrobio.2026.117352","DOIUrl":"10.1016/j.diagmicrobio.2026.117352","url":null,"abstract":"<div><div><em>Plasmodium</em> parasites are the causative agents of a life-threatening malaria disease, causing a substantial economic loss to the health sectors on a global scale, especially in Pakistan, due to the presence of <em>Plasmodium vivax</em> and <em>Plasmodium falciparum.</em> However, <em>P. vivax</em> is highly prevalent and responsible for approximately 75% malaria infection with limited reports on its genetic diversity. The current study analyzed the genetic diversity of the <em>P. vivax</em> merozoite surface protein 3α and 3β (Pvmsp-3α and β) gene in field isolates of Azad Jammu and Kashmir (AJK) from Pakistan. Blood samples (<em>n</em> = 207) were collected from malaria-suspected patients in health care sectors between March and August 2024 for microscopy and DNA extraction. A nested PCR was performed targeting the Pvmsp-3α and Pvmsp-3β genes, followed by restriction fragment length polymorphism (RFLP) using AluI and PstI restriction enzymes. All microscopically examined samples were positive (207). Among them, PCR assay detected <em>P. vivax</em> in 188/207 (90.82%) samples and <em>P. falciparum</em> in 19/207 (9.18%) samples. Of the PCR-confirmed <em>P. vivax</em> samples, 98/188 (52.13%) and 90/188 (47.87%) were Pvmsp-3α and Pvmsp-3β amplified fragments, showing three and two major allelic variants, respectively. In Pvmsp-3α, the allelic distribution was type A (65/98, 66.33%), type B (11/98, 11.22%), and type C (13/98, 13.27%). Similarly, the allelic frequencies of Pvmsp-3β for type A and B were 69/90 (76.67%) and 21/90 (23.33%), respectively. This exploration of the <em>P. vivax</em> genotypic diversity from AJK is a major contribution to the demonstration of its complexity and polymorphism. The outcomes of the present findings are helpful in understanding the molecular epidemiology of <em>P. vivax</em> and exploring mixed genotype infection for control strategies.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"115 3","pages":"Article 117352"},"PeriodicalIF":1.8,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147387752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the cefazolin inoculum effect in blood culture-isolated methicillin-susceptible Staphylococcus aureus strains: A Japanese multicenter study","authors":"Shinnosuke Fukushima ,&nbsp;Shuma Tsuji ,&nbsp;Kazuyoshi Gotoh ,&nbsp;Koji Iio ,&nbsp;Sakura Ogawa ,&nbsp;Norihito Koyanagi ,&nbsp;Yuji Ito ,&nbsp;Hiroshi Koganemaru ,&nbsp;Atsushi Yoshida ,&nbsp;Hideharu Hagiya","doi":"10.1016/j.diagmicrobio.2026.117345","DOIUrl":"10.1016/j.diagmicrobio.2026.117345","url":null,"abstract":"<div><h3>Background</h3><div>Cefazolin inoculum effect (CInE) is a microbiological phenomenon where the MIC of cefazolin against methicillin-susceptible <em>Staphylococcus aureus</em> (MSSA) strains increases with higher bacterial volumes.</div></div><div><h3>Method</h3><div>We retrospectively investigated the prevalence and characteristics of the CInE among MSSA strains isolated from blood cultures at three Japanese hospitals. The collected isolates were screened for <em>blaZ</em> using PCR, and the cefazolin minimum inhibitory concentration (MIC) for the <em>blaZ</em>-positive MSSA isolates was measured at standard and high inoculum volumes. CInE-positive MSSA strains were defined as those with a cefazolin MIC ≥16 μg/mL at 10⁷ CFU/mL and ≤8 μg/mL at 10⁵ CFU/mL. In these <em>blaZ</em>-positive strains, we performed <em>blaZ</em> typing and tested a modified nitrocefin-based rapid examination to detect the CInE.</div></div><div><h3>Results</h3><div>We collected 329 MSSA strains isolated from blood cultures. Of these, 96 (29.2%) were positive for the <em>bla</em>Z gene, with the following genotypes: type A (15, 15.6%), type B (3, 3.1%), type C (77, 80.2%), type D (0, 0.0%), and non-type (1, 1.0%). Among 96 <em>bla</em>Z-positive MSSA isolates, 11 exhibited the CInE, all of which harbored <em>blaZ</em> type A. The rapid nitrocefin test detected CInE positivity with high sensitivity (100%), specificity (94.1%), and diagnostic accuracy (94.8%).</div></div><div><h3>Conclusion</h3><div>This study highlighted the low prevalence of CInE-presenting MSSA isolates in Japan. When the cefazolin MIC is ≥1 μg/mL or the penicillin G MIC is ≥0.25 μg/mL, the rapid nitrocefin test may be useful for considering the CInE in patients with high bacterial volume MSSA infections.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"115 3","pages":"Article 117345"},"PeriodicalIF":1.8,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147364029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A case of Legionnaires’ disease linked to the household hot water system, confirmed by whole genome sequencing","authors":"Ani-Ioana Cotar ,&nbsp;Mihaela Oprea ,&nbsp;Sorin Dinu ,&nbsp;Laura-Ioana Popa ,&nbsp;Cătălin Herghelegiu ,&nbsp;Manuela-Luminița Minea ,&nbsp;Roxana-Elena Buzilă ,&nbsp;Alexandru Duhaniuc ,&nbsp;Odette Popovici","doi":"10.1016/j.diagmicrobio.2026.117357","DOIUrl":"10.1016/j.diagmicrobio.2026.117357","url":null,"abstract":"<div><div><em>Legionella pneumophila</em> – an opportunistic bacterial pathogen frequently identified in the environmental water samples – can produce human infections after inhalation of contaminated aerosols. We describe a case of Legionnaires’ disease linked to a household drinking water system. Serogroup 1 <em>L. pneumophila</em> isolates were recovered from both human and environmental samples and were analysed by whole genome sequencing (WGS). Phylogenetic relatedness inferred by core genome multi-locus sequence typing (cgMLST) revealed the household drinking water system as the source of isolates causing human infection. This epidemiological investigation performed in Romania and identifying the source of infection by WGS is the first of this kind for our country. The human and environmental isolates were undoubtedly linked through the use of high-discriminatory WGS analysis, which confirmed the relatedness inferred from the classical seven-loci sequence-based typing (SBT) scheme.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"115 3","pages":"Article 117357"},"PeriodicalIF":1.8,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147375867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does antibiotic therapy affect multiplex PCR results in community-acquired bacterial meningitis?","authors":"Caroline Reckart ,&nbsp;Elizabeth Aguilera ,&nbsp;Audrey Wanger ,&nbsp;Rafael Bravo-Santos ,&nbsp;Rodrigo Hasbun","doi":"10.1016/j.diagmicrobio.2026.117354","DOIUrl":"10.1016/j.diagmicrobio.2026.117354","url":null,"abstract":"","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"115 3","pages":"Article 117354"},"PeriodicalIF":1.8,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147387748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}