Diagnostic microbiology and infectious disease最新文献

{"title":"Global trends in the application of droplet digital PCR technology in the field of infectious disease pathogen diagnosis: A bibliometric analysis from 2012 to 2023","authors":"Xiaojie Yang ,&nbsp;Fengting Yu ,&nbsp;Chang Yan ,&nbsp;Mengying Li ,&nbsp;Xuelei Liang ,&nbsp;Xiaojie Lao ,&nbsp;Rui Sun ,&nbsp;Wenhao Lv ,&nbsp;Hanxi Zhang ,&nbsp;Fujie Zhang","doi":"10.1016/j.diagmicrobio.2024.116623","DOIUrl":"10.1016/j.diagmicrobio.2024.116623","url":null,"abstract":"<div><h3>Background</h3><div>This study discusses the development trends and current applications of digital droplet PCR technology in pathogen diagnostics.</div></div><div><h3>Methods</h3><div>A bibliometric analysis was conducted by retrieving droplet digital pcr related articles published between January 1,2012 to December31, 2023, from the Web of Science Core Collection.</div></div><div><h3>Results</h3><div>A total of 3,513 authors from 66 countries published 508 research papers across 203 academic institutions. The keyword clustering network shows that the main advantages of ddPCR are absolute quantification, high sensitivity, and high tolerance to PCR inhibitors. The keyword timeline shows that current research hotspots include continuous pathogen nucleic acid monitoring, trace sample detection, and resistance mutation identification.</div></div><div><h3>Conclusion</h3><div>The application of ddPCR in pathogen diagnosis is expanding, offering key advantages in absolute quantification, high sensitivity, and inhibitor tolerance, meeting emerging needs in nucleic acid monitoring and resistance detection.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"111 3","pages":"Article 116623"},"PeriodicalIF":2.1,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142756993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A 29-mRNA host response test to identify bacterial and viral infections and to predict 30-day mortality in emergency department patients with suspected infections: A prospective observational cohort study","authors":"Kirby Tong-Minh ,&nbsp;Leanne van Leeuwen ,&nbsp;Christian Ramakers ,&nbsp;Uan-I Chen ,&nbsp;Oliver Liesenfeld ,&nbsp;Diederik Gommers ,&nbsp;Eric van Gorp ,&nbsp;Henrik Endeman ,&nbsp;Yuri van der Does","doi":"10.1016/j.diagmicrobio.2024.116599","DOIUrl":"10.1016/j.diagmicrobio.2024.116599","url":null,"abstract":"<div><h3>Introduction</h3><div>The goal of this study is to validate the accuracy of the 29-mRNA host response classifiers Inflammatix Bacterial-Viral-Non infected-3b (IMX-BVN-3b) and Severity-3b (IMX-SEV-3b) to identify bacterial and viral infections and to predict 30-day mortality in patients with suspected infections in the ED.</div></div><div><h3>Methods</h3><div>This prospective observational cohort study enrolled patients with suspected infections in a tertiary ED. IMX-BVN-3b was compared to clinically forced and consensus adjudicated bacterial/viral infection status and IMX-SEV-3b was compared to 30-day mortality.</div></div><div><h3>Results</h3><div>A total of 688 patients were enrolled. Using forced adjudication, the AUC for the diagnosis of bacterial infection by IMX-BVN-3b was 0.76 (95 % CI: 0.72 – 0.80). The AUC for the diagnosis of viral infections was 0.89 (95 %CI 0.84-0.95). IMX-SEV-3b had an AUC of 0.77 (95 % CI: 0.68 – 0.85) on 30-day mortality.</div></div><div><h3>Conclusion</h3><div>The 29-gene host response classifiers IMX-BVN-3b and IMX-SEV-3b identify viral and bacterial infections and predict 30-day mortality in patients with suspected infections in the ED.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"111 3","pages":"Article 116599"},"PeriodicalIF":2.1,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142806204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tuberculous meningitis as an underlying cause of rapid neurological deterioration in a patient with a history of psychiatric disorder: Clinical case report","authors":"Fátima Paola Ávila Hernández ,&nbsp;Samuel Sevilla Fuentes ,&nbsp;Carmen Judith Serrano","doi":"10.1016/j.diagmicrobio.2024.116625","DOIUrl":"10.1016/j.diagmicrobio.2024.116625","url":null,"abstract":"<div><div>Tuberculous meningitis (TBM), the least common of extrapulmonary tuberculosis presentations, one of the most severe. Highly prevalent in immunosuppressed individuals it is associated with high mortality and significant neurological sequelae. TBM main complications are hydrocephalus, increased intracranial pressure, and compromised blood flow with poor prognosis. The diagnostic approach is complex, as the manifestations of TBM are nonspecific. In the case reported here, the first symptoms of TBM were masked by clinical manifestations of depressive syndrome (previously diagnosed) and its management with antidepressants. The confirmatory diagnosis was based on GenXpert on cerebrospinal fluid and neuroimaging, as in our case, tools frequently delayed. The patient presented sudden neurological deterioration, probably due to dysregulation of the immune system associated with depression and smoking habits. In areas with a high incidence of TB, neurological manifestations, even in patients with psychiatric disorders, should be considered as a differential diagnosis or infectious comorbidity such as TBM.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"111 3","pages":"Article 116625"},"PeriodicalIF":2.1,"publicationDate":"2024-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142747025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Catheter-related bloodstream infection by a newly proposed “Mycobacterium toneyamachuris” in a boy with B-cell precursor acute lymphoblastic leukemia","authors":"Haruki Naruse ,&nbsp;Noriyuki Watanabe ,&nbsp;Atsuhiko Ohta ,&nbsp;Yuuki Shimizu ,&nbsp;Takashi Fukushima ,&nbsp;Atsuko Watanabe ,&nbsp;Tomohei Nakao ,&nbsp;Yoshitada Taji ,&nbsp;Yuki Matsumoto ,&nbsp;Shota Nakamura ,&nbsp;Yuriko Igarashi ,&nbsp;Akiko Takaki ,&nbsp;Satoshi Mitarai ,&nbsp;Kotaro Mitsutake ,&nbsp;Ryuhei Tanaka ,&nbsp;Yasuhiro Ebihara","doi":"10.1016/j.diagmicrobio.2024.116626","DOIUrl":"10.1016/j.diagmicrobio.2024.116626","url":null,"abstract":"<div><div>This report describes a 6-year-old boy who developed non-tuberculosis mycobacteria (NTM) catheter-related bloodstream infection (CRBSI) during treatment for B-cell precursor acute lymphoblastic leukemia. A Hickman catheter was inserted before starting treatment. He developed a fever during chemotherapy, and blood culture was drawn from the catheter. Blurred Gram-positive bacilli were found in Gram stain, looked positive in Ziehl–Neelsen stain, and were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry as <em>Mycobacterium mucogenicum phocaicum</em> group. The administration of clarithromycin and amikacin was started and the Hickman catheter was removed. We performed genomic sequencing to obtain accurate identification, demonstrating that this microorganism was identified as “<em>M. toneyamachuris</em>” calculated by the average nucleotide identity index. Finally, a clinical diagnosis of CRBSI caused by “<em>M. toneyamachuris</em>” was made. Our findings suggest that genomic sequencing is available for the accurate identification of NTM species.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"111 3","pages":"Article 116626"},"PeriodicalIF":2.1,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142747026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cover 2 - Aims/Scopes, Ed Board","authors":"","doi":"10.1016/S0732-8893(24)00437-1","DOIUrl":"10.1016/S0732-8893(24)00437-1","url":null,"abstract":"","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"111 1","pages":"Article 116613"},"PeriodicalIF":2.1,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142700540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The first case of Aspergillus fumigatus with high-level resistance to voriconazole due to the TR46/Y121F/T289A mutation in the Russian Federation","authors":"Galina Klyasova,&nbsp;Anna Malchikova,&nbsp;Svetlana Khrulnova","doi":"10.1016/j.diagmicrobio.2024.116624","DOIUrl":"10.1016/j.diagmicrobio.2024.116624","url":null,"abstract":"<div><div>We report the first case of voriconazole-resistant <em>A. fumigatus</em> with the TR₄₆/Y121F/T289A mutation in the Russian Federation, identified in a 43-year-old woman with acute lymphoblastic leukemia after allogeneic hematopoietic stem cell transplantation.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"111 3","pages":"Article 116624"},"PeriodicalIF":2.1,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A non-autochthonous human rabies case in the historically rabies-free Andaman And Nicobar Islands, India","authors":"Arya Ramachandran ,&nbsp;Ashwini M Ananda ,&nbsp;Chakrakodi N Varun ,&nbsp;Dhanya K ,&nbsp;Avijit Roy ,&nbsp;Reeta S Mani","doi":"10.1016/j.diagmicrobio.2024.116627","DOIUrl":"10.1016/j.diagmicrobio.2024.116627","url":null,"abstract":"<div><div>Rabies, a fatal zoonotic viral disease claims numerous lives annually worldwide, particularly in Asia and Africa. Despite the high prevalence of rabies in India, the Andaman and Nicobar Islands have historically maintained a rabies-free status. This report documents the first non-indigenous case of human rabies in these islands, emphasizing the imperative for heightened surveillance measures.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"111 3","pages":"Article 116627"},"PeriodicalIF":2.1,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142747023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Letter to the Editor on article Scheurich G, Bhatnagar S, Westgard S. Sigma metrics analysis of serology screening assays to enhance quality and efficiency in New Zealand blood services. Diagnostic Microbiology & Infectious Disease 110 (2024)","authors":"Mohamed Mokhtar Khelil","doi":"10.1016/j.diagmicrobio.2024.116608","DOIUrl":"10.1016/j.diagmicrobio.2024.116608","url":null,"abstract":"","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"111 2","pages":"Article 116608"},"PeriodicalIF":2.1,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142746272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Successful treatment of infections caused by mycobacterium abscessus complex following aesthetic procedures: A case series in China","authors":"Jianping Zhu,&nbsp;Miaofa Ying,&nbsp;Rui Zhao,&nbsp;Zhenwei Yu","doi":"10.1016/j.diagmicrobio.2024.116621","DOIUrl":"10.1016/j.diagmicrobio.2024.116621","url":null,"abstract":"<div><h3>Background</h3><div>Aesthetic procedure-associated infections caused by <em>Mycobacterium abscessus</em> complex (MABC) have been an emerging concern. However, limited evidence is available on this topic, and there are no standard treatments. The selection of antimicrobial regimens for the effective treatment of MABC infections poses a significant challenge.</div></div><div><h3>Case presentation</h3><div>In this case series, we present three patients in China who developed MABC infections following various aesthetic procedures. Two patients presented with localized skin and soft tissue infections, whereas one patient developed a disseminated infection. Treatment involved a combination of intravenous amikacin and cefoxitin for more than two weeks, followed by an oral regimen comprising clarithromycin, linezolid, and moxifloxacin based on drug susceptibility testing results. Despite the treatment's efficacy, the patients experienced adverse reactions to the antibiotics, including gastrointestinal symptoms, anaemia, and hearing loss. All patients achieved successful outcomes with shorter treatment courses and no relapse during the 3-year follow-up period.</div></div><div><h3>Conclusion</h3><div>This case series emphasizes the importance of employing appropriate combination antibiotic therapies based on drug susceptibility testing results for aesthetic procedure-associated infection caused by MABC when specific subspecies are unidentified. The combination of intravenous aminoglycosides and cefoxitin, followed by oral sequential therapy, with the course of treatment specifically tailored to the severity of the infection, provides a valuable treatment reference for patients with MABC infections in China.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"111 2","pages":"Article 116621"},"PeriodicalIF":2.1,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142704324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic value of metagenomic next-generation sequencing for bronchoalveolar lavage diagnostics in patients with lower respiratory tract infections","authors":"Xiaojian Jiang ,&nbsp;Hua Guo ,&nbsp;Jia Sun ,&nbsp;Yuanlin Guan ,&nbsp;Ziyang Xie","doi":"10.1016/j.diagmicrobio.2024.116620","DOIUrl":"10.1016/j.diagmicrobio.2024.116620","url":null,"abstract":"<div><h3>Background</h3><div>Current diagnostic methods of lower respiratory tract infections (LRTIs) often lack specificity, underscoring the necessity for advanced technologies such as metagenomic next-generation sequencing (mNGS).</div></div><div><h3>Methods</h3><div>This retrospective study compared bronchoalveolar lavage fluid (BALF) analysis using mNGS and conventional microbiological tests (CMT) to evaluate their effectiveness in pathogen identification and alignment with clinical diagnoses.</div></div><div><h3>Results</h3><div>In this study involving 369 patients suspected of LTRIs, mNGS identified pathogens in 342 cases (92.7%), showing superior diagnostic performance compared to CMT (58.8%). The positive agreement and negative agreement rates of mNGS were 92.7% and 96.3%, respectively, both significantly higher than those of CMT (both p&lt;0.001). The sensitivity, specificity, positive predictive value, and negative predictive value of mNGS were significantly higher than those of CMT, with values of 99.7% vs. 57.1%, 68.4% vs. 26.3%, 96.5% vs. 87.1%, and 96.3% vs. 6.3%, respectively (all p&lt;0.001). Pathogen detection rates among the patients showed that 89.7% had evidence of LRTIs, with bacterial infections (20.1%), mycoplasma (13.6%), mycobacterium (4.3%), fungal (4.1%), viral (3.3%), and mixed infections (44.4%) being the most common. Furthermore, the study also differentiated the distribution of pathogens between adults and pediatric patients, and assessed the impact of pathogen types on severe outcomes using multivariate logistic regression, revealing that viral and fungal infections were more likely associated with severe symptoms, whereas mycoplasma infections typically presented with milder symptoms.</div></div><div><h3>Conclusions</h3><div>BALF mNGS proves effective for rapid, comprehensive pathogen detection in LRTIs, warranting its early use for enhanced diagnosis and management, especially across different age groups.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"111 2","pages":"Article 116620"},"PeriodicalIF":2.1,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142704323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}