Diagnostic microbiology and infectious disease最新文献

{"title":"Pathogen-specific biomarker profiles in periprosthetic joint infection","authors":"Yessica Zamudio-Cuevas ,&nbsp;Karina Martínez-Flores ,&nbsp;Rafael Franco-Cendejas ,&nbsp;Javier Fernández-Torres","doi":"10.1016/j.diagmicrobio.2025.117191","DOIUrl":"10.1016/j.diagmicrobio.2025.117191","url":null,"abstract":"<div><div>Serum and synovial biomarker levels are affected during periprosthetic joint infection (PJI), but it is unknown how different microorganisms influence these responses. The objective was to analyze serum levels of C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), and white blood cell (WBC) count, and synovial human β-defensin-1 (hBD-1) levels according to the type of microorganism isolated in patients with suspected PJI. In this retrospective observational study, 105 synovial samples were collected during revision arthroplasty, of which 64 were classified as PJI and 41 as aseptic failure (AF) according to MSIS criteria Serum CRP, ESR, WBC and synovial hBD-1 were quantified using standardized methods. Descriptive and comparative statistical analyses were performed using SPSS software, and <em>P</em>-values &lt;0.05 were considered significant. Seven bacterial species were identified (<em>Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus sp, Peptoniphilus assaccharolyticus, Finegoldia magna</em>, and <em>Morganella morganii</em>.) and no polymicrobial infections were detected. Synovial hBD-1 levels were significantly elevated in infections caused by <em>P. aeruginosa, S. aureus, S. epidermidis</em>, and <em>Enterococcus sp</em> (<em>P</em>&lt;0.00001). Similarly, serum CRP levels were higher in infections due <em>P. aeruginosa</em> and <em>S. epidermidis</em> (<em>P</em>&lt;0.00001), <em>S. aureus</em> (<em>P</em>=0.001), <em>Enterococcus sp</em> and <em>P. asaccharolyticus</em> (<em>P</em>&lt;0.0001), and <em>F. magna</em> (<em>P</em>=0.002). ESR and WBC counts also differed significantly from the AF group. These findings demonstrate that biomarker responses vary by pathogen, suggesting that pathogen-specific biomarker profiles could improve diagnostic accuracy, support early triage, and guide empirical antimicrobial therapy in PJI management. Incorporating these profiles into clinical decision-making may facilitate timely treatment initiation and personalized strategies for patient care. Further multicenter studies are warranted to validate these observations.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117191"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145603225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic insights into clinical Teichococcus aerofrigidensis: acquisition of aminoglycoside phosphotransferase resistance genes via Tn6082 transposon","authors":"Andrey Guimarães Sacramento ,&nbsp;João Pedro Rueda Furlan ,&nbsp;Roberto Vivas ,&nbsp;Cleverton Canuto Aragão ,&nbsp;Thais Martins-Gonçalves ,&nbsp;Gregory Batista Melocco ,&nbsp;Fernanda Esposito ,&nbsp;Elizabeth Harummyy Takagi ,&nbsp;Ana Paula Silva de Lemos ,&nbsp;Fabio Parra Sellera ,&nbsp;Nilton Lincopan","doi":"10.1016/j.diagmicrobio.2025.117239","DOIUrl":"10.1016/j.diagmicrobio.2025.117239","url":null,"abstract":"<div><div>A <em>Teichococcus aerofrigidensis</em> (basonym: <em>Roseomonas aerofrigidensis</em>) strain (ICBTA01) was isolated from a case of infective endocarditis in a patient with a complex cardiovascular medical record. Whole-genome sequencing confirmed that ICBTA01 is closely related to <em>T. aerofrigidensis</em>, although current ANI and dDDH thresholds could not be definitively applied due to the absence of type strain genomes; therefore, species-level designation is primarily supported by 16S rRNA identity. Genomic analysis predicted aminoglycoside phosphotransferase resistance genes <em>aph(3″)-Ib</em> and <em>aph(6)-Id</em> within a Tn<em>6082</em> transposon context, supporting horizontal transfer. This case highlights the relevance of genomic approaches for characterization of rare and emerging pathogens.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117239"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145836378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration of molecular diagnosis for HHV-6 encephalitis in immunocompetent individuals: A study combining mNGS and PCR","authors":"Jin Tang ,&nbsp;Zhilei Kang ,&nbsp;Shaoyong Cui ,&nbsp;Haoming Xu ,&nbsp;Hao Ren ,&nbsp;Ping Li ,&nbsp;Jingzhe Han ,&nbsp;Yueli Zou","doi":"10.1016/j.diagmicrobio.2025.117196","DOIUrl":"10.1016/j.diagmicrobio.2025.117196","url":null,"abstract":"<div><h3>Purpose</h3><div>To evaluate clinical features, diagnosis, treatment, and prognosis of HHV-6 encephalitis in immunocompetent adults, a rare and challenging condition.</div></div><div><h3>Methods</h3><div>In this retrospective, single-centre study, we enrolled six immunocompetent adults (mean age 60.67 ± 15.57 years) diagnosed with HHV-6 encephalitis at Hengshui People’s Hospital between January 2022 and December 2024. Clinical data, laboratory variables, cerebrospinal fluid (CSF) parameters, brain magnetic resonance imaging (MRI) findings, and electroencephalography (EEG) recordings were systematically collected. CSF samples underwent metagenomic next-generation sequencing (mNGS) for unbiased pathogen detection. All HHV-6-positive findings were subsequently confirmed by quantitative real-time polymerase chain reaction (PCR).</div></div><div><h3>Results</h3><div>HHV-6 encephalitis presented with fever, unconsciousness, convulsions, headache, and cognitive dysfunction. CSF analysis showed mild intracranial pressure elevation(median 186.67 mmH₂O, IQR 157.50-217.50mmH₂O), lymphocytic pleocytosis(58.6 ± 31.70 %), and normal or mildly elevated protein(0.234±0.99 g/L) and glucose(median 4.03 mmol/L, IQR 3.42-4.50 mmol/L) levels. MRI revealed high-FLAIR signals in the right temporal lobe in three patients and chronic foci in the frontal lobe and basal ganglia in three others. EEG abnormalities were noted in two patients. mNGS revealed an average of 578±512.09 unique HHV-6 reads, with HHV-6A identified in five patients and HHV-6B in one. All findings were confirmed by quantitative PCR. Prompt ganciclovir therapy led to complete recovery without sequelae in all six cases.</div></div><div><h3>Conclusions</h3><div>mNGS enables rapid detection of the rare but treatable HHV-6 meningitis in immunocompetent adults that conventional tests miss, prompting early antiviral therapy and better outcomes.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117196"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145570970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"False-positive results of the turbidimetric β-D-glucan assay in patients with bacteremia","authors":"Mario Fernández-Ruiz ,&nbsp;Ana Pérez-Ayala ,&nbsp;María Victoria Castaño Amores ,&nbsp;Fernando del Nogal Labrador ,&nbsp;María del Carmen Guevara Rodado ,&nbsp;María Asunción Pérez-Jacoiste Asín ,&nbsp;Isabel Rodríguez-Goncer ,&nbsp;Jose Tiago Silva ,&nbsp;Natalia Redondo ,&nbsp;Francisco López-Medrano ,&nbsp;Rafael San Juan ,&nbsp;José María Aguado","doi":"10.1016/j.diagmicrobio.2025.117186","DOIUrl":"10.1016/j.diagmicrobio.2025.117186","url":null,"abstract":"<div><h3>Background</h3><div>The presence of bacteremia is described as a cause of false-positive (FP) elevation of serum (1→3)-β-D-glucan (BDG) levels measured with the Fungitell colorimetric assay. The experience with the increasingly used turbidimetric Wako BDG assay in this scenario is limited.</div></div><div><h3>Methods</h3><div>We recruited 58 patients with proven bacteremia and no clinical suspicion of invasive fungal disease between October 2022 and July 2023. Serum BDG was assessed by the Wako test in blood samples collected immediately upon the confirmation of blood culture positivity (median interval of 3 days (interquartile range [IQR]: 2 – 5)). The positivity threshold was set at 7 pg/mL as per manufacturer’s instructions.</div></div><div><h3>Results</h3><div>The most common sources of bacteremia were urinary tract infection (27.6%) and intraabdominal or hepatobiliary infection (24.1%). Predominant pathogens included <em>Staphylococcus aureus</em> (44.1%), <em>Escherichia coli</em> (22.0%) and <em>Klebsiella pneumoniae</em> (15.5%). Most of the patients (91.4%) received a β-lactam-containing regimen. A positive BDG assay was obtained in 5 patients, yielding a FP rate of 8.6% (95% confidence interval: 2.9 – 18.9). The median BDG was 8.6 pg/mL (IQR: 7-9 – 12.4). All but one patient had at least one alternative cause of FP results in the BDG assay, including hemodialysis, colitis, albumin therapy and gauze exposure. One patient with persistently elevated BDG levels was eventually diagnosed with <em>Candida</em> esophagitis.</div></div><div><h3>Conclusions</h3><div>The occurrence of FP results with the turbidimetric BDG assay is uncommon, involves moderate reactivity and is typically accompanied by other potential causes of serum BDG elevation.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117186"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145555355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a universal real-time PCR TaqMan assay for fowl aviadenovirus diagnosis","authors":"Amina Kardoudi ,&nbsp;Siham Fellahi ,&nbsp;Faouzi Kichou ,&nbsp;Abdelmounaaim Allaoui ,&nbsp;Abdelouaheb Benani","doi":"10.1016/j.diagmicrobio.2025.117215","DOIUrl":"10.1016/j.diagmicrobio.2025.117215","url":null,"abstract":"<div><div>Fowl aviadenoviruses (FAdVs) are major pathogens of poultry, associated with economically important diseases such as Inclusion Body Hepatitis (IBH), Adenovirus Gizzard Erosion (AGE), and Hepatitis-Hydropericardium Syndrome (HHS). Accurate diagnosis is critical for disease control and surveillance, yet current methods are limited by serotype diversity. This study describes the development and validation of a universal TaqMan real-time PCR assay for the detection and quantification of multiple FAdV serotypes. Universal primers and a probe were designed to target a conserved region of the 52K gene and optimized for assay performance. Analytical evaluation demonstrated high efficiency (95 %), strong linearity (R² = 0.955), and a detection limit of 5 copies/mL. The assay showed no cross-reactivity with other avian pathogens, including avian influenza virus, Newcastle disease virus, infectious bronchitis virus, and infectious laryngotracheitis virus. Precision testing revealed minimal intra- and inter-assay variability, with coefficients of variation below 0.02 %, confirming robustness and reproducibility. Clinical evaluation was conducted on 56 field samples from confirmed IBH and AGE cases involving serotypes FAdV-1, -8a, -8b, and -11 as well as 14 samples from healthy chicken. The assay successfully amplified all positive samples and showed a strong correlation (R² = 0.97) with a previously established SYBR Green-based 52K real-time PCR assay. In conclusion, this novel TaqMan real-time PCR assay represents a rapid, sensitive, and highly specific diagnostic tool for FAdV detection. Its broad applicability across serotypes and strong clinical performance make it suitable for both routine diagnostics and epidemiological surveillance of FAdV infections in poultry.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117215"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modeling the economic impact of different testing strategies for meningitis/encephalitis in pediatric patients from a US Hospital Perspective","authors":"Rodrigo Hasbun ,&nbsp;Kyle D. Hueth ,&nbsp;Glaucia Paranhos-Baccala ,&nbsp;Lohit Korrapati ,&nbsp;Catherine Regan ,&nbsp;Adrienne Kwok ,&nbsp;Noam Kirson","doi":"10.1016/j.diagmicrobio.2025.117218","DOIUrl":"10.1016/j.diagmicrobio.2025.117218","url":null,"abstract":"<div><div>A pediatric-focused simulation model estimated that syndromic testing of suspected meningitis/encephalitis cases would result in cost savings to US hospitals of $542-$4,818 compared with rapid diagnostic testing and off-site-only testing, through more timely pathogen identification and reductions in hospital length of stay.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117218"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145862286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Borrelia crocidurae meningoencephalitis in a young Senegalense man returning to Tenerife, Canary Islands","authors":"Cristian Mateo León ,&nbsp;Sandra Rodríguez Martín ,&nbsp;Diego García Martínez de Artola ,&nbsp;Aránzazu Portillo ,&nbsp;Magdalena Lara Pérez ,&nbsp;Raquel Escudero ,&nbsp;Carlos Solé Violán ,&nbsp;Paula Santibáñez ,&nbsp;Jorge Martín Trueba ,&nbsp;José Antonio Oteo","doi":"10.1016/j.diagmicrobio.2025.117227","DOIUrl":"10.1016/j.diagmicrobio.2025.117227","url":null,"abstract":"<div><div><em>Borrelia crocidurae</em> infection is considered endemic in West Africa. We describe a <em>B. crocidurae</em> meningoencephalitis in a young Senegalese man returning to the Canary Islands from his home country. Clinical suspicion in patients from endemic areas and molecular diagnostic techniques are essential for accurate identification of this pathogen.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117227"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145780572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic potential of non-invasive tests for Helicobacter pylori-associated gastroduodenal disease in a resource-limited laboratory setting","authors":"Maryam Gasimova ,&nbsp;Akif Gurbanov ,&nbsp;Agigat Kadyrova ,&nbsp;Ravil Huseynov ,&nbsp;Vidadi Narimanov","doi":"10.1016/j.diagmicrobio.2025.117167","DOIUrl":"10.1016/j.diagmicrobio.2025.117167","url":null,"abstract":"<div><h3>Background</h3><div>In resource-limited laboratory settings non-invasive diagnostics are essential for <em>Helicobacter pylori</em> detection, yet their accuracy varies with disease severity. This study aimed to evaluate diagnostic potential of two widely available non-invasive diagnostic methods - serological antibody (Ab) detection and lateral flow stool-antigen testing (HpSAT) - for their ability both to detect and to differentiate <em>H. pylori</em>-associated gastroduodenal disorders.</div></div><div><h3>Methods</h3><div>A total of 128 symptomatic patients (98 with gastritis and 30 with stomach/duodenal ulcer) were enrolled. All underwent ELISA-IgG, rapid Ab test and HpSAT; 17 ulcer patients additionally had a biopsy rapid-urease test (RUT). Per-index composite reference standard (CRS) was established to define infection status. As a no-gold-standard sensitivity analysis, we implemented a Bayesian latent class model (LCM) with local dependence between ELISA and Rapid Ab test.</div></div><div><h3>Results</h3><div>Under the CRS, ELISA had sensitivity 98.2 % and specificity 62.5 %; Rapid Ab test had 91.2 % and 53.6 %; HpSAT had 63.5 % and 75.0 %. For ulcer vs. gastritis discrimination in the full cohort, HpSAT had the highest overall accuracy (65.6 %) via balanced sensitivity (76.7 %) and higher specificity (62.2 %). Bayesian LCM estimated Se/Sp as: ELISA Se/Sp 64.6 %/80.9 %; Rapid Ab 91.7 %/63.8 %; HpSAT 94.8 % /74.2 %).</div></div><div><h3>Conclusions</h3><div>HpSAT showed the most balanced non-invasive performance for detecting active <em>H. pylori</em> and the strongest discrimination of ulcer versus gastritis. Where urease breath testing (UBT) is unavailable, a tiered approach—HpSAT first-line for suspected active infection/ulceration, with serology as adjunct appears pragmatic. Prospective studies using a uniform reference standard are needed.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 2","pages":"Article 117167"},"PeriodicalIF":1.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145437405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pseudomonas guariconensis Necrotizing soft tissue infection, Denmark","authors":"Sofus Rønne Soltau ,&nbsp;Nina Elisabeth Pontoppidan Monrad ,&nbsp;Allan Evald Væver Nielsen ,&nbsp;Jacob Ehlers-Hansen ,&nbsp;Henrik Pierre Calum","doi":"10.1016/j.diagmicrobio.2025.117121","DOIUrl":"10.1016/j.diagmicrobio.2025.117121","url":null,"abstract":"<div><div>We describe the first published case report of a successful treatment of <em>Pseudomonas guariconensis</em>-induced Necrotizing Soft Tissue Infection (NSTI). Since <em>Pseudomonas guariconensis</em> has been resistant to meropenem in all reported cases, clinicians and microbiologists should treat these patients with extreme care.</div></div><div><h3>Patient and methods</h3><div>A 72-year-old man presented to the emergency department with a 3-day history of fever, pain in the right leg/foot and general malaise. He was diagnosed with NSTI, empirical meropenem and clindamycin was started, and surgery was quickly performed with removal of all dead skin and subcutaneous tissue. All fasciae were intact.</div></div><div><h3>Results</h3><div>Blood cultures and tissue samples showed growth of <em>Pseudomonas guariconensis</em>, which a literature review revealed to be highly suspicious of meropenem resistance. This prompted quick change of antibiotics, first with ciprofloxacin and metronidazole added and then change of meropenem to cefiderocol. Susceptibility tests on both blood and tissue samples revealed resistance to meropenem (along with resistance to many other antibiotics). The patient improved, was changed to ceftazidim and ciprofloxacin and recovered fully from the infection.</div></div><div><h3>Conclusions</h3><div>Since clindamycin has no effect on any <em>Pseudomonas species</em> and since <em>Pseudomonas guariconensis</em> has been resistant to meropenem in all reported cases, clinicians and microbiologists should treat these patients with extreme care.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 2","pages":"Article 117121"},"PeriodicalIF":1.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145227328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the QIAstat-Dx® Gastrointestinal Panel 2 for detection of viral, bacterial and parasitic pathogens in fecal swab specimens","authors":"Mélissa Caza ,&nbsp;Cherie Tantichalermsin ,&nbsp;Sherry Gonzales ,&nbsp;Catherine A. Hogan ,&nbsp;David Su ,&nbsp;Jennifer Grant ,&nbsp;Amanda Wilmer","doi":"10.1016/j.diagmicrobio.2025.117129","DOIUrl":"10.1016/j.diagmicrobio.2025.117129","url":null,"abstract":"<div><div>This study evaluates the performance of the QIAstat-Dx® gastrointestinal panel 2 (GIP2) (Qiagen, Spain) for the gastrointestinal pathogens from Fecalswab® specimens (Copan, Italy). For most targets, specimens were tested initially on the Allplex^TM GI assays (Seegene, South Korea). For <em>P. shigelloides</em>, specimens were initially tested on BIOFIRE® GI Panel (BGIP) (bioMérieux, France). Two hundred fifty-nine specimens were tested on the GIP2 (69.5 % archived, 30.5 % prospective). Specimens with discordant results on Allplex^TM GI assays were tested on BGIP or Xpert® Norovirus (Cepheid, USA), while discordant results for P<em>. shigelloides</em> were tested by culture. Consensus results were obtained based on agreement of at least 2 of 3 tests. The percent positive and negative agreement (PPA, PNA) were calculated on a per target basis. Compared to consensus, GIP2 PPA was 95.02 % (95 % CI 91.78 – 97.25 %) and PNA was 99.98 % (95 % CI 99.90- 100.00 %). GIP2 performed well for bacterial and parasitic targets with PPA and PNA &gt;97 %, however had a PPA of 88.3 % (95 % CI 80-81 – 93.61 %) and PNA of 100.0 % (95 % CI 99.75 – 100.00 %) for viral targets. When CT values were examined, QIAstat-dx GIP2 produce similar values to the Allplex^TM GI-viral assay. However, bacterial and parasitic targets on QIAstat-dx GIP2 trends towards lower CT values when compared to the Allplex^TM GI assays.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 2","pages":"Article 117129"},"PeriodicalIF":1.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145227330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}