Diagnostic microbiology and infectious disease最新文献

{"title":"Multi-centre clinical validation of the NeuMoDx HPV assay for ASC-US / LSIL triage","authors":"Taeyang Chin ,&nbsp;Anja Oštrbenk ,&nbsp;Nick Verhavert ,&nbsp;Mario Poljak ,&nbsp;Elizaveta Padalko","doi":"10.1016/j.diagmicrobio.2025.117208","DOIUrl":"10.1016/j.diagmicrobio.2025.117208","url":null,"abstract":"<div><div>The NeuMoDx™ HPV assay (QIAGEN, MI, USA) is a PCR assay that detects 15 high-risk human papillomavirus (HPV) types in cervical samples, with separate identification for HPV16 and HPV18. Although it has been validated for primary HPV-based cervical cancer screening, its performance in the triage of atypical squamous cells of undetermined significance (ASC-US) or low-grade squamous intraepithelial lesion (LSIL) in cytology-based screening algorithms has not yet been evaluated. This multicentre study assessed the clinical performance of the NeuMoDx assay for ASC-US/LSIL triage. A total of 447 residual clinician-collected cervical samples were analysed. At site 1 (Ljubljana, Slovenia), NeuMoDx was compared with the Alinity m HR HPV assay (Abbott Molecular, IL, USA), and at site 2 (Ghent, Belgium), it was compared with the APTIMA HPV assay (HOLOGIC, MA, USA). NeuMoDx demonstrated 91.3% (95% CI: 85.8-94.7%) concordance with Alinity and 96.2% (95% CI: 93.3-97.8%) concordance with APTIMA, with excellent kappa values of 0.824 (95% CI:0.737-0.912) for site 1 and 0.779 (95% CI: 0.653-0.905) for site 2. The relative sensitivity of NeuMoDx for detecting cervical intraepithelial neoplasia grade 2 or worse (CIN2+) was 0.91 and 1.08 compared to Alinity and Aptima, respectively. Relative clinical specificity of NeuMoDx was 1.05 compared to Alinity and 1.00 compared to Aptima. In conclusion, the NeuMoDx HPV assay showed reliable clinical performance in triaging ASC-US/LSIL samples.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117208"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145676884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three new single nucleotide polymorphisms identified in Plasmodium falciparum isolates from Kogi State, Nigeria","authors":"Onyekachi Nnaemeka Akoma ,&nbsp;Anthony Chibuogwu Ike ,&nbsp;Ebere Emilia Ayogu ,&nbsp;Martina Chinonye Agbo ,&nbsp;Chukwuemeka Cornelius Ezeh ,&nbsp;Felicia Ngozi Okoh ,&nbsp;Solomon Odiba Arome ,&nbsp;Ifeoma Maureen Ezeonu","doi":"10.1016/j.diagmicrobio.2025.117233","DOIUrl":"10.1016/j.diagmicrobio.2025.117233","url":null,"abstract":"<div><h3>Background</h3><div>High malaria burden remains a remarkable challenge in Nigeria. The high prevalence has been attributed to a number of factors, including the continued evolution of resistance to antimalarial drugs by <em>Plasmodium</em>. However, the actual occurrence of antimalarial drug resistance in the country has not been well-documented. This study was aimed at screening for molecular markers of multidrug resistant <em>Plasmodium</em> species in individuals with parasitaemia in Kogi State, Nigeria.</div></div><div><h3>Method</h3><div>Blood samples drawn from 600 symptomatic patients who consented to be recruited for the study were tested by Dipstick antigen test (RDT) for malaria. Genomic DNA from 40 samples, confirmed positive for <em>P. falciparum</em> by the rapid diagnostic test and PCR, were screened for the <em>Pfmdr1</em> and <em>Kelch-13</em> genes. Some of the <em>Kelch-13</em> PCR amplicons were randomly selected and sequenced using Sanger method. Thereafter, the sequences were analysed and compared with wild type for single nucleotide polymorphisms (SNPs).</div></div><div><h3>Results</h3><div><em>Pfmdr1</em> and <em>K-13</em> amplicons were successfully obtained from 45 % (18/40) of the malaria-positive blood samples. Bioinformatics analysis of the <em>K-13</em> sequences showed near 100 % sequence homology and percentage similarity of 99.88 % with the wild type <em>Plasmodium falciparum</em> 3D7 (Accession Number XM_001350122) gene. There were however three non-synonymous mutations (K430E, W706C and Q707K) observed in three samples.</div></div><div><h3>Conclusions</h3><div>SNP analysis showed none of these mutations has been previously associated with artemisinin resistance. The significance of the study results was discussed in relation to current national malaria elimination and antimalarial resistance strategic plan or policy.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117233"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145833423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of P30 adhesin detected by ultrasensitive ELISA in the clinical diagnosis and management of Mycoplasma pneumoniae","authors":"Po-Kai Chen ,&nbsp;Kan-Hsuan Lin ,&nbsp;Guan-Ming Ke ,&nbsp;Shun-Cheng Yang ,&nbsp;Chao-Jen Lin ,&nbsp;Cheng-Han Lee ,&nbsp;Rei-Cheng Yang ,&nbsp;Chien-Sheng Hsu ,&nbsp;Tsung-Ying Yang ,&nbsp;Mitsumasa Hasunuma ,&nbsp;Keita Sudo ,&nbsp;Etsuro Ito ,&nbsp;Jun-Kai Kao","doi":"10.1016/j.diagmicrobio.2025.117192","DOIUrl":"10.1016/j.diagmicrobio.2025.117192","url":null,"abstract":"<div><h3>Background</h3><div><em>Mycoplasma pneumoniae</em> (MP) is a common cause of pediatric respiratory infections, but its diagnosis remains challenging due to nonspecific clinical features and the limitations of conventional laboratory tests.</div></div><div><h3>Methods</h3><div>We evaluated the diagnostic and clinical utility of P30 detection using the ultrasensitive TN-cyclon™ assay in 67 hospitalized children with respiratory tract infections. Diagnostic accuracy was compared with bacterial culture, respiratory PCR, and serum IgM testing. Associations between P30 levels and clinical course, laboratory findings, co-infection status, radiographic severity, and macrolide resistance were also analyzed.</div></div><div><h3>Results</h3><div>P30 TN-cyclon™ showed strong diagnostic performance (sensitivity 83.33 %, specificity 92.31 %), outperforming serum IgM (sensitivity 48.08 %) while remaining slightly less sensitive than PCR (sensitivity 93.94 %) but comparable in overall diagnostic performance. ROC analysis yielded an AUC of 0.892, with an optimal cutoff of 0.4 pg/mL for MP infection. Patients with co-infections had lower mean P30 levels than those with mono-infection, though not statistically significant. P30 concentrations were not associated with radiographic severity. In macrolide-resistant cases, higher P30 levels were observed, with ROC analysis identifying 2.45 pg/mL as a potential threshold (AUC = 0.66).</div></div><div><h3>Conclusions</h3><div>P30 is a reliable biomarker for the rapid diagnosis of MP, with superior accuracy to serology and practical advantages over PCR in clinical settings. While its predictive value for macrolide resistance is modest, integration with other biomarkers may enhance its clinical utility. TN-cyclon™ offers a promising tool for timely management of MP infections in children.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117192"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145555353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathogen-specific biomarker profiles in periprosthetic joint infection","authors":"Yessica Zamudio-Cuevas ,&nbsp;Karina Martínez-Flores ,&nbsp;Rafael Franco-Cendejas ,&nbsp;Javier Fernández-Torres","doi":"10.1016/j.diagmicrobio.2025.117191","DOIUrl":"10.1016/j.diagmicrobio.2025.117191","url":null,"abstract":"<div><div>Serum and synovial biomarker levels are affected during periprosthetic joint infection (PJI), but it is unknown how different microorganisms influence these responses. The objective was to analyze serum levels of C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), and white blood cell (WBC) count, and synovial human β-defensin-1 (hBD-1) levels according to the type of microorganism isolated in patients with suspected PJI. In this retrospective observational study, 105 synovial samples were collected during revision arthroplasty, of which 64 were classified as PJI and 41 as aseptic failure (AF) according to MSIS criteria Serum CRP, ESR, WBC and synovial hBD-1 were quantified using standardized methods. Descriptive and comparative statistical analyses were performed using SPSS software, and <em>P</em>-values &lt;0.05 were considered significant. Seven bacterial species were identified (<em>Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus sp, Peptoniphilus assaccharolyticus, Finegoldia magna</em>, and <em>Morganella morganii</em>.) and no polymicrobial infections were detected. Synovial hBD-1 levels were significantly elevated in infections caused by <em>P. aeruginosa, S. aureus, S. epidermidis</em>, and <em>Enterococcus sp</em> (<em>P</em>&lt;0.00001). Similarly, serum CRP levels were higher in infections due <em>P. aeruginosa</em> and <em>S. epidermidis</em> (<em>P</em>&lt;0.00001), <em>S. aureus</em> (<em>P</em>=0.001), <em>Enterococcus sp</em> and <em>P. asaccharolyticus</em> (<em>P</em>&lt;0.0001), and <em>F. magna</em> (<em>P</em>=0.002). ESR and WBC counts also differed significantly from the AF group. These findings demonstrate that biomarker responses vary by pathogen, suggesting that pathogen-specific biomarker profiles could improve diagnostic accuracy, support early triage, and guide empirical antimicrobial therapy in PJI management. Incorporating these profiles into clinical decision-making may facilitate timely treatment initiation and personalized strategies for patient care. Further multicenter studies are warranted to validate these observations.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117191"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145603225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic insights into clinical Teichococcus aerofrigidensis: acquisition of aminoglycoside phosphotransferase resistance genes via Tn6082 transposon","authors":"Andrey Guimarães Sacramento ,&nbsp;João Pedro Rueda Furlan ,&nbsp;Roberto Vivas ,&nbsp;Cleverton Canuto Aragão ,&nbsp;Thais Martins-Gonçalves ,&nbsp;Gregory Batista Melocco ,&nbsp;Fernanda Esposito ,&nbsp;Elizabeth Harummyy Takagi ,&nbsp;Ana Paula Silva de Lemos ,&nbsp;Fabio Parra Sellera ,&nbsp;Nilton Lincopan","doi":"10.1016/j.diagmicrobio.2025.117239","DOIUrl":"10.1016/j.diagmicrobio.2025.117239","url":null,"abstract":"<div><div>A <em>Teichococcus aerofrigidensis</em> (basonym: <em>Roseomonas aerofrigidensis</em>) strain (ICBTA01) was isolated from a case of infective endocarditis in a patient with a complex cardiovascular medical record. Whole-genome sequencing confirmed that ICBTA01 is closely related to <em>T. aerofrigidensis</em>, although current ANI and dDDH thresholds could not be definitively applied due to the absence of type strain genomes; therefore, species-level designation is primarily supported by 16S rRNA identity. Genomic analysis predicted aminoglycoside phosphotransferase resistance genes <em>aph(3″)-Ib</em> and <em>aph(6)-Id</em> within a Tn<em>6082</em> transposon context, supporting horizontal transfer. This case highlights the relevance of genomic approaches for characterization of rare and emerging pathogens.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117239"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145836378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration of molecular diagnosis for HHV-6 encephalitis in immunocompetent individuals: A study combining mNGS and PCR","authors":"Jin Tang ,&nbsp;Zhilei Kang ,&nbsp;Shaoyong Cui ,&nbsp;Haoming Xu ,&nbsp;Hao Ren ,&nbsp;Ping Li ,&nbsp;Jingzhe Han ,&nbsp;Yueli Zou","doi":"10.1016/j.diagmicrobio.2025.117196","DOIUrl":"10.1016/j.diagmicrobio.2025.117196","url":null,"abstract":"<div><h3>Purpose</h3><div>To evaluate clinical features, diagnosis, treatment, and prognosis of HHV-6 encephalitis in immunocompetent adults, a rare and challenging condition.</div></div><div><h3>Methods</h3><div>In this retrospective, single-centre study, we enrolled six immunocompetent adults (mean age 60.67 ± 15.57 years) diagnosed with HHV-6 encephalitis at Hengshui People’s Hospital between January 2022 and December 2024. Clinical data, laboratory variables, cerebrospinal fluid (CSF) parameters, brain magnetic resonance imaging (MRI) findings, and electroencephalography (EEG) recordings were systematically collected. CSF samples underwent metagenomic next-generation sequencing (mNGS) for unbiased pathogen detection. All HHV-6-positive findings were subsequently confirmed by quantitative real-time polymerase chain reaction (PCR).</div></div><div><h3>Results</h3><div>HHV-6 encephalitis presented with fever, unconsciousness, convulsions, headache, and cognitive dysfunction. CSF analysis showed mild intracranial pressure elevation(median 186.67 mmH₂O, IQR 157.50-217.50mmH₂O), lymphocytic pleocytosis(58.6 ± 31.70 %), and normal or mildly elevated protein(0.234±0.99 g/L) and glucose(median 4.03 mmol/L, IQR 3.42-4.50 mmol/L) levels. MRI revealed high-FLAIR signals in the right temporal lobe in three patients and chronic foci in the frontal lobe and basal ganglia in three others. EEG abnormalities were noted in two patients. mNGS revealed an average of 578±512.09 unique HHV-6 reads, with HHV-6A identified in five patients and HHV-6B in one. All findings were confirmed by quantitative PCR. Prompt ganciclovir therapy led to complete recovery without sequelae in all six cases.</div></div><div><h3>Conclusions</h3><div>mNGS enables rapid detection of the rare but treatable HHV-6 meningitis in immunocompetent adults that conventional tests miss, prompting early antiviral therapy and better outcomes.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117196"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145570970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"False-positive results of the turbidimetric β-D-glucan assay in patients with bacteremia","authors":"Mario Fernández-Ruiz ,&nbsp;Ana Pérez-Ayala ,&nbsp;María Victoria Castaño Amores ,&nbsp;Fernando del Nogal Labrador ,&nbsp;María del Carmen Guevara Rodado ,&nbsp;María Asunción Pérez-Jacoiste Asín ,&nbsp;Isabel Rodríguez-Goncer ,&nbsp;Jose Tiago Silva ,&nbsp;Natalia Redondo ,&nbsp;Francisco López-Medrano ,&nbsp;Rafael San Juan ,&nbsp;José María Aguado","doi":"10.1016/j.diagmicrobio.2025.117186","DOIUrl":"10.1016/j.diagmicrobio.2025.117186","url":null,"abstract":"<div><h3>Background</h3><div>The presence of bacteremia is described as a cause of false-positive (FP) elevation of serum (1→3)-β-D-glucan (BDG) levels measured with the Fungitell colorimetric assay. The experience with the increasingly used turbidimetric Wako BDG assay in this scenario is limited.</div></div><div><h3>Methods</h3><div>We recruited 58 patients with proven bacteremia and no clinical suspicion of invasive fungal disease between October 2022 and July 2023. Serum BDG was assessed by the Wako test in blood samples collected immediately upon the confirmation of blood culture positivity (median interval of 3 days (interquartile range [IQR]: 2 – 5)). The positivity threshold was set at 7 pg/mL as per manufacturer’s instructions.</div></div><div><h3>Results</h3><div>The most common sources of bacteremia were urinary tract infection (27.6%) and intraabdominal or hepatobiliary infection (24.1%). Predominant pathogens included <em>Staphylococcus aureus</em> (44.1%), <em>Escherichia coli</em> (22.0%) and <em>Klebsiella pneumoniae</em> (15.5%). Most of the patients (91.4%) received a β-lactam-containing regimen. A positive BDG assay was obtained in 5 patients, yielding a FP rate of 8.6% (95% confidence interval: 2.9 – 18.9). The median BDG was 8.6 pg/mL (IQR: 7-9 – 12.4). All but one patient had at least one alternative cause of FP results in the BDG assay, including hemodialysis, colitis, albumin therapy and gauze exposure. One patient with persistently elevated BDG levels was eventually diagnosed with <em>Candida</em> esophagitis.</div></div><div><h3>Conclusions</h3><div>The occurrence of FP results with the turbidimetric BDG assay is uncommon, involves moderate reactivity and is typically accompanied by other potential causes of serum BDG elevation.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117186"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145555355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a universal real-time PCR TaqMan assay for fowl aviadenovirus diagnosis","authors":"Amina Kardoudi ,&nbsp;Siham Fellahi ,&nbsp;Faouzi Kichou ,&nbsp;Abdelmounaaim Allaoui ,&nbsp;Abdelouaheb Benani","doi":"10.1016/j.diagmicrobio.2025.117215","DOIUrl":"10.1016/j.diagmicrobio.2025.117215","url":null,"abstract":"<div><div>Fowl aviadenoviruses (FAdVs) are major pathogens of poultry, associated with economically important diseases such as Inclusion Body Hepatitis (IBH), Adenovirus Gizzard Erosion (AGE), and Hepatitis-Hydropericardium Syndrome (HHS). Accurate diagnosis is critical for disease control and surveillance, yet current methods are limited by serotype diversity. This study describes the development and validation of a universal TaqMan real-time PCR assay for the detection and quantification of multiple FAdV serotypes. Universal primers and a probe were designed to target a conserved region of the 52K gene and optimized for assay performance. Analytical evaluation demonstrated high efficiency (95 %), strong linearity (R² = 0.955), and a detection limit of 5 copies/mL. The assay showed no cross-reactivity with other avian pathogens, including avian influenza virus, Newcastle disease virus, infectious bronchitis virus, and infectious laryngotracheitis virus. Precision testing revealed minimal intra- and inter-assay variability, with coefficients of variation below 0.02 %, confirming robustness and reproducibility. Clinical evaluation was conducted on 56 field samples from confirmed IBH and AGE cases involving serotypes FAdV-1, -8a, -8b, and -11 as well as 14 samples from healthy chicken. The assay successfully amplified all positive samples and showed a strong correlation (R² = 0.97) with a previously established SYBR Green-based 52K real-time PCR assay. In conclusion, this novel TaqMan real-time PCR assay represents a rapid, sensitive, and highly specific diagnostic tool for FAdV detection. Its broad applicability across serotypes and strong clinical performance make it suitable for both routine diagnostics and epidemiological surveillance of FAdV infections in poultry.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117215"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modeling the economic impact of different testing strategies for meningitis/encephalitis in pediatric patients from a US Hospital Perspective","authors":"Rodrigo Hasbun ,&nbsp;Kyle D. Hueth ,&nbsp;Glaucia Paranhos-Baccala ,&nbsp;Lohit Korrapati ,&nbsp;Catherine Regan ,&nbsp;Adrienne Kwok ,&nbsp;Noam Kirson","doi":"10.1016/j.diagmicrobio.2025.117218","DOIUrl":"10.1016/j.diagmicrobio.2025.117218","url":null,"abstract":"<div><div>A pediatric-focused simulation model estimated that syndromic testing of suspected meningitis/encephalitis cases would result in cost savings to US hospitals of $542-$4,818 compared with rapid diagnostic testing and off-site-only testing, through more timely pathogen identification and reductions in hospital length of stay.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117218"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145862286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Borrelia crocidurae meningoencephalitis in a young Senegalense man returning to Tenerife, Canary Islands","authors":"Cristian Mateo León ,&nbsp;Sandra Rodríguez Martín ,&nbsp;Diego García Martínez de Artola ,&nbsp;Aránzazu Portillo ,&nbsp;Magdalena Lara Pérez ,&nbsp;Raquel Escudero ,&nbsp;Carlos Solé Violán ,&nbsp;Paula Santibáñez ,&nbsp;Jorge Martín Trueba ,&nbsp;José Antonio Oteo","doi":"10.1016/j.diagmicrobio.2025.117227","DOIUrl":"10.1016/j.diagmicrobio.2025.117227","url":null,"abstract":"<div><div><em>Borrelia crocidurae</em> infection is considered endemic in West Africa. We describe a <em>B. crocidurae</em> meningoencephalitis in a young Senegalese man returning to the Canary Islands from his home country. Clinical suspicion in patients from endemic areas and molecular diagnostic techniques are essential for accurate identification of this pathogen.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117227"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145780572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}