Diagnostic microbiology and infectious disease最新文献

{"title":"Impact of delayed incubation on mycobacterial blood culture recovery with the BACTEC Myco/F Lytic system","authors":"Emmanuel Lecorche ,&nbsp;Typhaine Billard-Pomares ,&nbsp;Fatma Magdoud-El Alaoui ,&nbsp;Emmanuelle Gallois ,&nbsp;Vincent Fihman ,&nbsp;Amélie Carrër ,&nbsp;Laura Djamdjian ,&nbsp;François Camelena ,&nbsp;Faiza Mougari ,&nbsp;Etienne Carbonnelle ,&nbsp;Paul-Louis Woerther","doi":"10.1016/j.diagmicrobio.2025.117217","DOIUrl":"10.1016/j.diagmicrobio.2025.117217","url":null,"abstract":"<div><div>This study assessed the impact of delayed incubation on mycobacterial blood culture accuracy. We performed an experimental study and a 5-year retrospective analysis of 1592 clinical vials categorized by preincubation time. Delayed incubation for up to 3 days was not associated with lower detection rates of mycobacteria.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117217"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145755624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spontaneous reverse seroconversion in resolved hepatitis B infection","authors":"Aybegüm Özşahin ,&nbsp;Tuba İlgar","doi":"10.1016/j.diagmicrobio.2025.117194","DOIUrl":"10.1016/j.diagmicrobio.2025.117194","url":null,"abstract":"","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117194"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145603168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression profiles of GPR56 and CD99 in T cells of AIDS patients and assessment of diagnostic value","authors":"Yuanzhong Sun ,&nbsp;Yuan Yuan ,&nbsp;Xueli Chen ,&nbsp;Yuemei Liang ,&nbsp;Yugui Su ,&nbsp;Liling Yang ,&nbsp;Qingyang Zhong ,&nbsp;Suidong Ouyang ,&nbsp;Xuerong Sun ,&nbsp;Minnan Ye ,&nbsp;Pingzhang Wang ,&nbsp;Wenrui Li","doi":"10.1016/j.diagmicrobio.2025.117222","DOIUrl":"10.1016/j.diagmicrobio.2025.117222","url":null,"abstract":"<div><h3>Objectives</h3><div>GPR56, an adhesion G protein-coupled receptor, and CD99, a cell-surface glycoprotein, have emerged as important immunomodulators in HIV infection. GPR56 plays a crucial role in T cell migration, activation, and the interaction between the virus and the host cell. Concurrently, CD99 is implicated in regulating key processes such as T cell adhesion, migration, and apoptotic signaling. This study aimed to investigate their dynamic expression in T-cell subsets as potential biomarkers for AIDS progression and diagnosis.</div></div><div><h3>Methods</h3><div>Differentially expressed genes in T cells were screened using single-cell RNA sequencing. Experimental validation was performed by flow cytometry on peripheral blood samples from 36 PLWH (male:31, female:5, age: 41.58±12.42) and 35 healthy controls (male:25, female:10, age: 32.17±12.09). Systematically evaluated the protein expression profiles of GPR56 and CD99 on the surface of T cells. The Pearson method assessed correlation, and receiver operating characteristic (ROC) analysis evaluated diagnostic performance.</div></div><div><h3>Results</h3><div>GPR56 expression on CD8⁺ T cells increased significantly with disease progression (<em>P</em> &lt; 0.05), while CD99 expression remained stable across stages. The CD8⁺GPR56⁺CD99⁺ T-cell subset demonstrated high diagnostic accuracy for HIV infection (AUC = 0.90), with frequencies that increased progressively with disease severity. Moderate co-expression of GPR56 and CD99 was observed in CD8⁺ T cells in both cohorts. In contrast, CD4⁺GPR56⁺ T cells showed no discriminative capacity between early-stage patients and controls.</div></div><div><h3>Conclusions</h3><div>GPR56 and CD99 exhibit distinct expression patterns during HIV infection. The CD8⁺GPR56⁺ and CD8⁺GPR56⁺CD99⁺ T-cell subsets serve as potent stage-specific biomarkers, with combined GPR56/CD99 analysis enhancing diagnostic precision for advanced AIDS.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117222"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights on vaccine-derived poliovirus type 2 outbreaks and novel oral polio vaccine candidates","authors":"Khaled M. Aboshanab ,&nbsp;Mohammad Y. Alshahrani ,&nbsp;Hesham Ali El Enshasy ,&nbsp;Menna R Shabana ,&nbsp;Abu Bakar Abdul Majeed","doi":"10.1016/j.diagmicrobio.2025.117221","DOIUrl":"10.1016/j.diagmicrobio.2025.117221","url":null,"abstract":"<div><div>Despite the life-saving benefits of the vaccination with the live attenuated oral polio vaccine, or Sabin (OPV), OPV still evolves rapidly to lose attenuating determinants critical to the reacquisition of virulence, resulting in vaccine-derived, virulent poliovirus variants. The circulation of these variants within under-immunized populations leads to the further evolution of circulating, vaccine-derived poliovirus with increased transmission capacity, representing a significant risk of polio re-emergence. Although the wild poliovirus type 2 (WPV2) had been declared as eradicated worldwide in 2015, outbreaks of circulating VDPV-type 2 (cVDPV2) have been increasingly reported, especially after the tOPV-bOPV switch that occurred in 2016, which resulted in building a cohort of susceptible individuals with lower type 2 intestinal immunity. Since the response to outbreaks is done through conducting supplementary immunization activities (SIAs) using type-2 containing OPV, this carries a risk of mutations, especially with suboptimum coverage and potential further circulation. The recent detection of outbreaks of cVDPV2 in some countries necessitated the development of a genetically stable novel oral polio vaccine type 2 (nOPV-2) to decrease the chances of mutations and hence outbreaks. This review highlights the recent outbreaks of VDPV serotypes, emphasizing the role of the World Health Organization and the Global Polio Eradication Initiative in detecting and controlling VDPV outbreaks. The emerging VDPV, strategies implemented, reported clinical trials, and proposals for developing nOPV. The way the review is presented will encourage assertiveness, assist in posing open-ended questions, and provide several ideas for further research.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117221"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid detection of respiratory syncytial virus using RT-LAMP-CRISPR/Cas12b on a gravity-driven microfluidic chip","authors":"Yuanzhu Liu ,&nbsp;Haokun Mei ,&nbsp;Chang Gao ,&nbsp;Yingqi Yang","doi":"10.1016/j.diagmicrobio.2025.117244","DOIUrl":"10.1016/j.diagmicrobio.2025.117244","url":null,"abstract":"<div><h3>Aims</h3><div>Respiratory syncytial virus (RSV) is the most significant pathogen causing acute lower respiratory tract infections in children. Early detection of RSV can help control disease progression and reduce complications. However, RT-qPCR-based detection methods cannot provide accurate results within one hour and are not suitable for use in resource-limited settings. Therefore, there is a pressing need to develop a rapid and precise bedside RSV detection assay to meet clinical demands.</div></div><div><h3>Methods and results</h3><div>Firstly, we developed a RT-LAMP-assisted CRISPR/Cas12b method for detecting RSV M gene, capable of identifying target RNA with a limit of detection as low as 100 copies/μL within 40 min. Secondly, we created and tested a sampling lysis reagent, demonstrating its effectiveness in enabling direct detection without the need for nucleic acid extraction, thus improving bedside detection efficiency. Lastly, to facilitate use in resource-limited areas, we designed and developed a gravity-driven microfluidic chip that simplifies the stepwise process of RT-LAMP amplification and CRISPR/Cas12b detection. This chip allows for visual recognition of results without the need for an external power source when used in point-of-care (POC) settings. This assay showed 99% agreement with RT-qPCR, highlighting its potential for practical application. Additionally, no cross-reactivity was observed with other respiratory pathogens infection, demonstrating good clinical specificity.</div></div><div><h3>Conclusions</h3><div>In summary, the platform we developed is faster and more user-friendly than RT-qPCR, while achieving comparable sensitivity.</div></div><div><h3>Impact statement</h3><div>Our finding fills the gap in the inability to detect RSV in POC setting, safeguarding the health of children, and offers new insights for the innovation of CRISPR diagnostics.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117244"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145880495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of a novel viral nucleic acid extraction kit from a variety of clinical specimens","authors":"Wanxian Hong ,&nbsp;Weihua Luo ,&nbsp;Lijia Mao ,&nbsp;Zihuan Lu ,&nbsp;Wenbo Wang ,&nbsp;Xinqiang Zhang ,&nbsp;Yanhui Wu ,&nbsp;Genquan Yin ,&nbsp;Lihong Sun","doi":"10.1016/j.diagmicrobio.2025.117241","DOIUrl":"10.1016/j.diagmicrobio.2025.117241","url":null,"abstract":"<div><div>Nucleic acid extraction represents a critical preanalytical step in clinical molecular diagnostics. This study evaluated a novel viral nucleic acid extraction kit (HeSheng) against three commercial kits (Qiagen QIAamp, Daan, and Yocon) using 170 clinical samples across five specimen types. Performance was assessed through plaque-based serial dilution combined with RT-qPCR to determine the limit of detection (LOD), alongside measurements of nucleic acid concentration, OD260/280 ratio, and Ct values. While all kits demonstrated equivalent LODs (10 PFU/mL), the HeSheng kit consistently yielded lower Ct values, indicating enhanced sensitivity. It also produced nucleic acids with superior concentration, purity, and amplification efficiency. Clinical sample RT-qPCR results showed high concordance with diagnoses (sensitivity 98.6%; specificity 97.4%). The protocol combines viral lysis with rapid column-based adsorption and completes extraction within 10 minutes using standard equipment. These findings demonstrate that the HeSheng kit provides a simplified, cost-effective, and broadly applicable method suitable for molecular diagnostics in resource-limited or high-throughput settings.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117241"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145854675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A cold-chain free loop-mediated isothermal amplification test for detection of human malaria in point-of-care settings","authors":"Nurul Nabila Rosli ,&nbsp;Yee Ling Lau ,&nbsp;Meng Yee Lai","doi":"10.1016/j.diagmicrobio.2025.117237","DOIUrl":"10.1016/j.diagmicrobio.2025.117237","url":null,"abstract":"<div><div>Malaria diagnosis in low-resource settings demands robust and cold-chain free molecular tests. To overcome infrastructure limitations of conventional loop mediated isothermal amplification (LAMP), we developed a refrigeration-independent assay for <em>Plasmodium knowlesi</em> detection. By using sugar-based enzyme preservation approach, sucrose shows most effectively to maintain <em>Bst</em> DNA polymerase activity during room-temperature storage, outperforming equivalent concentrations of glucose and galactose. The sucrose-preserved LAMP reagent demonstrated consistent performance for 12 days. This optimized assay achieved analytical sensitivity (10 copies/μL) and maintained 100 % specificity against <em>Plasmodium</em> species and human DNA. The chemical preserving method provided a cost-effective alternative to lyophilization, eliminating dependence on specialized equipment while achieving comparable short-term stability. The 12-day room-temperature stability suits typical field deployments, making the assay ideal for malaria surveillance and use in remote clinics.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117237"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145854726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multicenter verification and method comparison of the STANDARD M10 Influenza, RSV and SARS-CoV-2 assay in Amies medium","authors":"Pieter-Jan Briers ,&nbsp;Hanne Valgaeren ,&nbsp;Dagmar Obbels ,&nbsp;Paul-Emile Claus ,&nbsp;Annick Smismans ,&nbsp;Ellen Van Even ,&nbsp;Wim Laffut ,&nbsp;Ann Lemmens ,&nbsp;Truus Goegebuer ,&nbsp;Bea Van den Poel ,&nbsp;Johan Frans","doi":"10.1016/j.diagmicrobio.2025.117230","DOIUrl":"10.1016/j.diagmicrobio.2025.117230","url":null,"abstract":"<div><div>The STANDARD M10 Flu/RSV/SARS-CoV-2 (M10) is an all-in-one RT-PCR assay which can simultaneously differentiate and detect SARS-CoV-2, influenza A (InfA), influenza B (InfB) and respiratory syncytial virus (RSV). This study describes a multicenter verification of the M10 assay and is the first study to report a method comparison performed on nasopharyngeal swabs collected in Amies medium (eSwab, Copan) which isn’t a manufacturer approved medium. The multicenter verification was performed in four Belgian Hospital laboratories. The accuracy was determined on 28 external quality assessment samples. The reproducibility was assessed using a COVFLU control sample. The method comparison was conducted at the Imelda Hospital where 26 positive and 40 negative samples analyzed with the Magpix NxTAG Respiratory pathogen panel + and 14 positive samples analyzed with Xpert Xpress SARS-CoV-2/Flu/RSV assay were retrospectively analyzed with the M10 assay. Regarding the multicenter verification, the accuracy was 100 % and the M10 assay systematically detected the four pathogens from the COVFLU control. The method comparison yielded positive and negative percent agreements of 100 %, except for InfB the positive percent agreement between the M10 and the Xpert assay was 83.3 %. These results are in line with the results of other studies evaluating the performance of the M10 assay on manufacturers approved media. It is concluded that M10 assay can be used for nasopharyngeal samples collected in Amies medium.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117230"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145786877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical and molecular characterization of a fulminant meningococcemia case in an adult caused by Neisseria meningitidis serogroup Y clonal complex 23 strain in China","authors":"Yueshan Liang ,&nbsp;Liangcai Xu ,&nbsp;Song Liu ,&nbsp;Zheng Liang ,&nbsp;Biyu Lin ,&nbsp;Jiaqi Ye ,&nbsp;Shuping Nie","doi":"10.1016/j.diagmicrobio.2025.117242","DOIUrl":"10.1016/j.diagmicrobio.2025.117242","url":null,"abstract":"<div><h3>Background</h3><div><em>Neisseria meningitidis serogroup Y</em> (NmY), first reported in China in 2015, remains rare, with only a few cases reported to date. Previously documented NmY invasive meningococcal disease (IMD) cases in China predominantly occurred among adolescents or children. This study reports the first confirmed adult IMD case caused by NmY in China.</div></div><div><h3>Methods</h3><div>A 22-year-old male in Guangdong Province presented with IMD symptoms. The causative agent (NmY20231109) was isolated from blood culture. Antimicrobial susceptibility testing was performed. Whole-genome sequencing (WGS) was conducted for molecular characterization and phylogenetic analysis against global NmY strains.</div></div><div><h3>Results</h3><div>The isolate (NmY20231109) was susceptible to most tested antibiotics but resistant to sulfamethoxazole/trimethoprim. WGS identified the strain as Y: P1.5-1,10-1: F4-1: ST-1655 (CC23). Phylogenetic analysis revealed that NmY20231109 clustered closely with ST-1655 strains from the UK and Germany. It showed genetic distance from the four ST-1655 strains previously reported in China. Virulence gene profiling identified 52 associated genes, including key factors such as fHbp and porB.</div></div><div><h3>Conclusion</h3><div>This report describes the first adult IMD case caused by NmY in China. The identification of NmY20231109 as ST-1655 (CC23), closely related to European strains, suggests a potential imported origin and highlights the ongoing risk of NmY transmission in China.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117242"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145880493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-centre clinical validation of the NeuMoDx HPV assay for ASC-US / LSIL triage","authors":"Taeyang Chin ,&nbsp;Anja Oštrbenk ,&nbsp;Nick Verhavert ,&nbsp;Mario Poljak ,&nbsp;Elizaveta Padalko","doi":"10.1016/j.diagmicrobio.2025.117208","DOIUrl":"10.1016/j.diagmicrobio.2025.117208","url":null,"abstract":"<div><div>The NeuMoDx™ HPV assay (QIAGEN, MI, USA) is a PCR assay that detects 15 high-risk human papillomavirus (HPV) types in cervical samples, with separate identification for HPV16 and HPV18. Although it has been validated for primary HPV-based cervical cancer screening, its performance in the triage of atypical squamous cells of undetermined significance (ASC-US) or low-grade squamous intraepithelial lesion (LSIL) in cytology-based screening algorithms has not yet been evaluated. This multicentre study assessed the clinical performance of the NeuMoDx assay for ASC-US/LSIL triage. A total of 447 residual clinician-collected cervical samples were analysed. At site 1 (Ljubljana, Slovenia), NeuMoDx was compared with the Alinity m HR HPV assay (Abbott Molecular, IL, USA), and at site 2 (Ghent, Belgium), it was compared with the APTIMA HPV assay (HOLOGIC, MA, USA). NeuMoDx demonstrated 91.3% (95% CI: 85.8-94.7%) concordance with Alinity and 96.2% (95% CI: 93.3-97.8%) concordance with APTIMA, with excellent kappa values of 0.824 (95% CI:0.737-0.912) for site 1 and 0.779 (95% CI: 0.653-0.905) for site 2. The relative sensitivity of NeuMoDx for detecting cervical intraepithelial neoplasia grade 2 or worse (CIN2+) was 0.91 and 1.08 compared to Alinity and Aptima, respectively. Relative clinical specificity of NeuMoDx was 1.05 compared to Alinity and 1.00 compared to Aptima. In conclusion, the NeuMoDx HPV assay showed reliable clinical performance in triaging ASC-US/LSIL samples.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117208"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145676884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}