Diagnostic microbiology and infectious disease最新文献

{"title":"Post-surgical mediastinitis caused by Aspergillus calidoustus in a heart transplant recipient: A case report and review of the literature","authors":"Patricia Martínez-Martín ,&nbsp;Sandra Rosillo Rodríguez ,&nbsp;Irene Marco Clement ,&nbsp;Alejandro Díez-Vidal ,&nbsp;Inmaculada Quiles-Melero ,&nbsp;Bunty Ramchandani ,&nbsp;Alicia Rico ,&nbsp;Belén Loeches","doi":"10.1016/j.diagmicrobio.2025.116796","DOIUrl":"10.1016/j.diagmicrobio.2025.116796","url":null,"abstract":"<div><div>Acute mediastinitis is a common infectious complication following heart transplant surgery. The etiology is typically bacterial, and the condition tends to have a favourable outcome with appropriate source control and antimicrobial treatment. Clinically, it often presents acutely, with early dehiscence of the surgical wound and systemic involvement, necessitating prompt surgical intervention. Fungal etiology is rare and usually associated with severely immunocompromised patients, conferring a dismal prognosis. Although most fungal infections are caused by <em>Aspergillus fumigatus</em>, the widespread use of azoles as prophylactic treatment has led to an increase in infections by unknown species and other azole-resistant fungi, posing a significant therapeutic challenge.</div><div>We present the case of a 31-year-old heart transplant recipient who developed post-surgical mediastinitis due to <em>Aspergillus calidoustus</em> following a complicated postoperative course and humoral rejection. Despite appropriate antimicrobial therapy and source control, the infection progressed unfavourably.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 2","pages":"Article 116796"},"PeriodicalIF":2.1,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143609959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-infection of intestinal protozoa and intestinal parasites with Helicobacter pylori among HIV AIDS patients at Jimma Medical Center, Ethiopia","authors":"Tolera Ambisa Lamesa","doi":"10.1016/j.diagmicrobio.2025.116789","DOIUrl":"10.1016/j.diagmicrobio.2025.116789","url":null,"abstract":"<div><h3>Background</h3><div>Human Immunodeficiency Viruses (HIV) infected people are at increased chance of immunocompromission. In combination with this immunocompromission, the incidence of co-infection with intestinal parasites, intestinal protozoal and <em>Helicobacter pylori</em> can be escalated by different factors including similar mode of transmission, poor economic status, synergistic pathogenesis, and geographic topography of more than two organisms. A large number of world population is either affected or at risk of infection from diversified intestinal parasites, intestinal protozoal and <em>H. pylori</em>.</div></div><div><h3>Objective</h3><div>The study determined the prevalence of H. pylori with intestinal parasites and intestinal protozoal co-infection among HIV positive individuals and factors associated to the co-infection at Jimma University Medical Center (JUMC); Jimma, Ethiopia.</div></div><div><h3>Materials and methods</h3><div>This cross-sectional study included 384 HIV positive patients using consecutive sampling technique. Stool sample was examined using normal saline to diagnosis intestinal parasites and with lugol's Iodine for active motile intestinal protozoa and the stool sample was concentrated by formalin-ethyl acetate and stained with Kinyoun-modified acid-fast stain to stain coccidian protozoa <em>H. pylori</em> was diagnosed by antigen test using Wondfo one Step <em>H. pylori</em> feces test.</div></div><div><h3>Result</h3><div>In this study, gender distribution was 177(46.1 %) males and 207(53.9 %) females. About 179 were between 10 and 20 years. The prevalence of <em>H. pylori</em>, intestinal parasites and intestinal protozoal infection among HIV positive individuals were 27.6 % 24.4 % and 6.5 % respectively. The overall prevalence of both intestinal parasites and intestinal protozoal co-infection with <em>H. pylori</em> was 20.7 %. Positive associations of co-infection with source of drinking water, animal contact, finger nail trimming, economic status and shoe-wearing habits as significant variables.</div></div><div><h3>Conclusion and recommendation</h3><div>A notable co-infection involving <em>H. Pylori</em> alongside intestinal parasites and intestinal protozoa was identified. It was acknowledged that the sanitary and socio-economic conditions could serve as potential risk factors associated with co-infection. Enhancing hygiene and sanitation practices is essential to interrupt the transmission pathways of intestinal parasites, <em>H. Pylori</em>, and intestinal protozoa, particularly for individuals with weakened immune systems, including those living with AIDS.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 2","pages":"Article 116789"},"PeriodicalIF":2.1,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143629518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First human case of infected lymphocele with bloodstream infection caused by Staphylococcus coagulans transmitted from a pet dog: a case report","authors":"Hiroyuki Kitano ,&nbsp;Hiroki Kitagawa ,&nbsp;Junzo Hisatsune ,&nbsp;Manami Tsunoi ,&nbsp;Yuki Kohada ,&nbsp;Kenshiro Takemoto ,&nbsp;Shunsuke Miyamoto ,&nbsp;Kohei Kobatake ,&nbsp;Kayoko Tadera ,&nbsp;Keitaro Omori ,&nbsp;Motoyuki Sugai ,&nbsp;Hiroki Ohge ,&nbsp;Nobuyuki Hinata","doi":"10.1016/j.diagmicrobio.2025.116792","DOIUrl":"10.1016/j.diagmicrobio.2025.116792","url":null,"abstract":"<div><div>We report the first case of a lymphocele and bloodstream infection caused by <em>S. coagulans</em> post-surgery. Single nucleotide polymorphism-based analyses revealed closely related <em>S. coagulans</em> isolates from the patient and his dog. Using whole genome sequencing, we demonstrated that <em>S. coagulans</em> was transmitted from a dog.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 3","pages":"Article 116792"},"PeriodicalIF":2.1,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiotics beyond bacteria: Novel immunomodulation strategies for periodontal diseases","authors":"Mohammad Amin Amiri ,&nbsp;Shahram Hamedani ,&nbsp;Farnaz Haji Abbas Oghli","doi":"10.1016/j.diagmicrobio.2025.116793","DOIUrl":"10.1016/j.diagmicrobio.2025.116793","url":null,"abstract":"","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 2","pages":"Article 116793"},"PeriodicalIF":2.1,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143636957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism of Helicobacter pylori vacuolar cytotoxin a-induced gastric mucosal atrophy: A histopathological and immunohistochemical analysis","authors":"Yang-Kun Wang ,&nbsp;Shen-Lin Li ,&nbsp;Kai Zhang ,&nbsp;Yong-Jue Liu ,&nbsp;Ying-Ying Li ,&nbsp;Fa-Shun Zhang ,&nbsp;Yao-Dong Niu ,&nbsp;Chao-Ya Zhu ,&nbsp;Su-Nan Wang ,&nbsp;Si-Liang Xu","doi":"10.1016/j.diagmicrobio.2025.116785","DOIUrl":"10.1016/j.diagmicrobio.2025.116785","url":null,"abstract":"<div><h3>Objective</h3><div>This study aims to examine the underlying mechanism of gastric mucosal atrophy induced by vacuolar cytotoxin A (VacA) produced by <em>Helicobacter pylori</em>.</div></div><div><h3>Methods</h3><div>A total of 942 endoscopic biopsy and endoscopic submucosal dissection samples of gastric mucosa infected with <em>H. pylori</em> were subjected to detailed histomorphological and immunohistochemical analysis.</div></div><div><h3>Results</h3><div><em>H. pylori</em> exhibited specific adherence to surface mucus cells, proliferating extensively while producing and secreting VacA. The atrophic process was initiated by the upward migration and compensatory proliferation of cells in the deeper regions of the gastric pit, isthmus, and mucous neck cells. VacA disrupted the normal physiological organization and polarity of the proliferative zone, altering the proliferation patterns and directional growth of stem cells. This disruption resulted in a disordered state of cell proliferation.</div><div>Insufficient downward migration of cells within the proliferative zone led to atrophy of the lamina propria glands in the gastric mucosa. This process was accompanied by epithelial cell proliferation and transformation, along with interstitial infiltration of lymphocytes, a small number of plasma cells, and neutrophils. These histopathological changes ultimately contributed to the characteristic atrophic gastritis associated with <em>H. pylori</em> infection.</div></div><div><h3>Conclusion</h3><div>A comprehensive understanding of the histopathological features of VacA-induced gastric mucosal atrophy is essential for the prevention and management of <em>H. pylori</em>-related gastric carcinogenesis.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 2","pages":"Article 116785"},"PeriodicalIF":2.1,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143629519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection, relatedness and environmental sources of emerging Legionella longbeachae infections in Finland, 1989-2024","authors":"Silja Mentula ,&nbsp;Taru Miller ,&nbsp;Jenni Ikonen ,&nbsp;Piia Airaksinen ,&nbsp;Ellinoora Savonen ,&nbsp;Marjo Niittynen","doi":"10.1016/j.diagmicrobio.2025.116788","DOIUrl":"10.1016/j.diagmicrobio.2025.116788","url":null,"abstract":"<div><div><em>Legionella longbeachae</em> is an emerging cause of Legionnaires' disease in Europe.</div><div>We analysed data from the National Infectious Disease Register (NIDR) and the Legionella Reference Laboratory database to characterize L. longbeachae infections and diagnostics in Finland.</div><div>A total of 41 L<em>. longbeachae</em> patient cases, mainly gardeners’ pneumonias and hand wounds, were reported during 1989-2024, with a significant increase after 2019. Patient interviews and environmental sampling linked infections to compost, potting soil or outdoor activities. Whole genome sequencing (WGS) confirmed identical patient and soil isolates in three cases and revealed several clusters among non-related isolates.</div><div>Compared to infections caused by other Legionella species, L<em>. longbeachae</em> cases had distinct characteristics, including higher median age (64 vs. 56 yrs.), higher fatality (15 % vs. 8 %), more extrapulmonary infections (12 % vs. 1 %) and higher proportion of females (44 % vs. 35 %).</div><div>The genetic relatedness of patient and environmental isolates and high clonality suggest common environmental reservoirs.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 2","pages":"Article 116788"},"PeriodicalIF":2.1,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143591403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-sensitivity BK virus detection system using viewRNA in situ hybridization","authors":"Chunlan Hu ,&nbsp;Xiaonan Zhang ,&nbsp;Tongyu Zhu ,&nbsp;Yumin Hou ,&nbsp;Yejing Shi ,&nbsp;Jiajia Sun ,&nbsp;Nannan Wu","doi":"10.1016/j.diagmicrobio.2025.116790","DOIUrl":"10.1016/j.diagmicrobio.2025.116790","url":null,"abstract":"<div><h3>Objective</h3><div>To establish a reliable molecular diagnostic system for precise identification of BK virus (BKV)-associated pathologies.</div></div><div><h3>Methods</h3><div>We designed a set of <em>in-situ</em> hybridization (ISH) probes targeting two conserved sequences within the large T antigen region of BKV, exhibiting highly specificity from JC virus and SV40 homologs. By integrating the branched DNA signal amplification technology with these probes, we developed a novel ViewRNA-ISH detection system.</div></div><div><h3>Results</h3><div>The optimized BKV-LT ViewRNA-ISH system demonstrated enhanced diagnostic specificity and sensitivity compared to conventional immunohistochemical methods in both cellular models and clinical specimens. Validation using 29 clinical samples and 3 subcutaneous planted tumor samples revealed 93.75 % (30/32) concordance with IHC findings, with 2 previously IHC-negative renal biopsies showing positive signals through ViewRNA-ISH detection.</div></div><div><h3>Conclusions</h3><div>Our BKV-LT ViewRNA-ISH system enables dual-mode (fluorescent/ chemical) detection of BKV nucleic acids, providing superior diagnostic performance for BKV-associated pathologies with potential clinical utility in renal allograft monitoring.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 2","pages":"Article 116790"},"PeriodicalIF":2.1,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143577959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of Mycoplasma pneumoniae and Chlamydia pneumoniae using a laboratory-developed high-throughput test for the Panther Fusion system","authors":"Stephen Strunge Nilsson,&nbsp;Linda Fährmann,&nbsp;Thomas Sundelin,&nbsp;Lene Nielsen","doi":"10.1016/j.diagmicrobio.2025.116786","DOIUrl":"10.1016/j.diagmicrobio.2025.116786","url":null,"abstract":"<div><div><em>Mycoplasma pneumoniae</em> and <em>Chlamydia pneumoniae</em> are key agents of atypical pneumonia. During a 2023 <em>M. pneumoniae</em> outbreak in Denmark, a high-throughput laboratory-developed test (LDT) was created for the Hologic Panther Fusion system using Open Access software. This multiplex real-time PCR assay employs TaqMan® probes targeting the CARDS tx-gene (<em>M. pneumoniae</em>) and argR gene (<em>C. pneumoniae</em>). Validation included external quality controls, defined bacterial concentrations, and frozen patient samples. Sensitivity, specificity, precision, and robustness were assessed, with 95 % limits of detection at 111 and 878 copies/mL for M. pneumoniae and C. pneumoniae, respectively. Specificity was 100 % against 30 related pathogens. Over the course of 2024 we used the assay routinely analysing 56,773 samples finding a positive prevalence of 18.9 % for <em>M. pneumoniae</em> and 1.5 % for <em>C. pneumoniae.</em> The assay demonstrated high sensitivity and specificity, offering rapid sample processing.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 2","pages":"Article 116786"},"PeriodicalIF":2.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143548222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cover 2 - Aims/Scopes, Ed Board","authors":"","doi":"10.1016/S0732-8893(25)00098-7","DOIUrl":"10.1016/S0732-8893(25)00098-7","url":null,"abstract":"","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 1","pages":"Article 116775"},"PeriodicalIF":2.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143549708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive surgical and medical management of massive fungal endocarditis in a preterm neonate: A rare case of survival and full recovery","authors":"Reyhaneh Alipoor Rafie ,&nbsp;Maedeh Soflaee ,&nbsp;Hojjat Mortezaeian ,&nbsp;Amirhossein Jalali ,&nbsp;Aydin Feyzi ,&nbsp;Mohsen Anafje ,&nbsp;Amirali Soheili","doi":"10.1016/j.diagmicrobio.2025.116787","DOIUrl":"10.1016/j.diagmicrobio.2025.116787","url":null,"abstract":"<div><h3>Background</h3><div>Fungal endocarditis in neonates, often caused by Candida albicans, is a rare but severe condition linked to invasive medical interventions. Preterm infants are particularly vulnerable due to their immature immune systems. Diagnosis is challenging due to nonspecific symptoms, and management typically requires both antifungal therapy and surgery.</div></div><div><h3>Case report</h3><div>A 43-day-old preterm female infant, born at 34 weeks with intrauterine growth restriction, presented with respiratory distress. She had been treated for suspected sepsis and found to have large tricuspid vegetations. Blood cultures confirmed Candida albicans. Despite initial antifungal treatment, surgery was performed due to the size of the vegetations. After surgery, the patient was treated with caspofungin, leading to clinical improvement. At 9-month follow-up, she showed full recovery with normal cardiac function.</div></div><div><h3>Conclusion</h3><div>This case demonstrates the successful management of neonatal fungal endocarditis through early diagnosis, surgical intervention, and appropriate antifungal therapy, leading to full recovery in a preterm neonate.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 2","pages":"Article 116787"},"PeriodicalIF":2.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143628617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}