由于磷脂酶活性弱，单核细胞增生李斯特菌诊断假阴性的风险

IF 1.8 4区医学 Q3 INFECTIOUS DISEASES

Diagnostic microbiology and infectious disease Pub Date : 2025-07-24 DOI:10.1016/j.diagmicrobio.2025.117024

Lauren V. Alteio , Ariane Pietzka , Laura van den Höövel , Patrick Mikuni-Mester

{"title":"由于磷脂酶活性弱，单核细胞增生李斯特菌诊断假阴性的风险","authors":"Lauren V. Alteio , Ariane Pietzka , Laura van den Höövel , Patrick Mikuni-Mester","doi":"10.1016/j.diagmicrobio.2025.117024","DOIUrl":null,"url":null,"abstract":"<div><div>We investigated an atypical <em>Listeria monocytogenes</em> strain isolated from the food processing environment, which may evade widely used diagnostic approaches. After species confirmation using PCR and MALDI-TOF, enzyme analysis revealed reduced phospholipase activity, indicating a link between its activity and the white appearance of atypical strains on chromogenic agar. While no mutation was detected in the phospholipase C gene, comparative genomics revealed a distinct seven base pair insertion in the <em>prfA</em> gene, resulting in gene truncation. These results highlight the risk of false negatives in current diagnostics and a need for improved detection methods to ensure food safety.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"113 4","pages":"Article 117024"},"PeriodicalIF":1.8000,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Risk of false-negative diagnostics of Listeria monocytogenes due to weak phospholipase activity\",\"authors\":\"Lauren V. Alteio , Ariane Pietzka , Laura van den Höövel , Patrick Mikuni-Mester\",\"doi\":\"10.1016/j.diagmicrobio.2025.117024\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>We investigated an atypical <em>Listeria monocytogenes</em> strain isolated from the food processing environment, which may evade widely used diagnostic approaches. After species confirmation using PCR and MALDI-TOF, enzyme analysis revealed reduced phospholipase activity, indicating a link between its activity and the white appearance of atypical strains on chromogenic agar. While no mutation was detected in the phospholipase C gene, comparative genomics revealed a distinct seven base pair insertion in the <em>prfA</em> gene, resulting in gene truncation. These results highlight the risk of false negatives in current diagnostics and a need for improved detection methods to ensure food safety.</div></div>\",\"PeriodicalId\":11329,\"journal\":{\"name\":\"Diagnostic microbiology and infectious disease\",\"volume\":\"113 4\",\"pages\":\"Article 117024\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2025-07-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Diagnostic microbiology and infectious disease\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0732889325003475\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Diagnostic microbiology and infectious disease","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0732889325003475","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}

引用次数: 0

摘要

我们研究了一株从食品加工环境中分离出来的非典型单核细胞增生李斯特菌，该菌株可能逃避了广泛使用的诊断方法。在用PCR和MALDI-TOF进行物种确认后，酶分析显示磷脂酶活性降低，表明其活性与非典型菌株在显色琼脂上的白色外观有关。虽然在磷脂酶C基因中未检测到突变，但比较基因组学显示prfA基因中有明显的7个碱基对插入，导致基因截断。这些结果突出了当前诊断中存在假阴性的风险，以及改进检测方法以确保食品安全的必要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Risk of false-negative diagnostics of Listeria monocytogenes due to weak phospholipase activity

We investigated an atypical Listeria monocytogenes strain isolated from the food processing environment, which may evade widely used diagnostic approaches. After species confirmation using PCR and MALDI-TOF, enzyme analysis revealed reduced phospholipase activity, indicating a link between its activity and the white appearance of atypical strains on chromogenic agar. While no mutation was detected in the phospholipase C gene, comparative genomics revealed a distinct seven base pair insertion in the prfA gene, resulting in gene truncation. These results highlight the risk of false negatives in current diagnostics and a need for improved detection methods to ensure food safety.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Diagnostic microbiology and infectious disease 医学-传染病学

CiteScore

5.30

自引率

3.40%

发文量

149

审稿时长

56 days

期刊介绍： Diagnostic Microbiology and Infectious Disease keeps you informed of the latest developments in clinical microbiology and the diagnosis and treatment of infectious diseases. Packed with rigorously peer-reviewed articles and studies in bacteriology, immunology, immunoserology, infectious diseases, mycology, parasitology, and virology, the journal examines new procedures, unusual cases, controversial issues, and important new literature. Diagnostic Microbiology and Infectious Disease distinguished independent editorial board, consisting of experts from many medical specialties, ensures you extensive and authoritative coverage.