Diagnostic microbiology and infectious disease最新文献

{"title":"Strengthening molecular testing capacity in Colombia: Challenges and opportunities","authors":"Milena Camargo ,&nbsp;Marina Muñoz ,&nbsp;Luz Helena Patiño ,&nbsp;Juan David Ramírez","doi":"10.1016/j.diagmicrobio.2025.116716","DOIUrl":"10.1016/j.diagmicrobio.2025.116716","url":null,"abstract":"<div><div>The COVID-19 pandemic has accelerated efforts to enhance pathogen detection using molecular biology techniques. This study examines the expansion of molecular testing capacity in Colombia, identifying strengths and areas for improvement in the existing infrastructure. The study began with the creation of a database inventorying laboratories based on publicly available data from government entities and active web searches. Ten laboratories were selected for detailed characterization. Structured surveys assessed their testing capacity and progress in implementing molecular-based diagnostic tests for various infectious diseases. The strategy for identifying laboratories showed a total of 311 laboratories. Of these, 65 % (<em>n</em> = 202) are private and 21 % (<em>n</em> = 65) are state-owned, mainly public health laboratories, and the remaining 14 % (<em>n</em> = 44) are affiliated with academic institutions. The highest concentration of these labs is in Bogotá, Antioquia, and Valle del Cauca, primarily in urban areas. Key limitations affecting testing laboratories in Colombia include: i) infrastructure (26.2 %), highlighting the need for standardized facility guidelines; ii) quality and documentation (16.7 %), requiring stronger quality management systems; iii) biosafety (14.3 %), emphasizing the need for continuous waste management, especially in public labs; and iv) human talent (10.7 %), needing better policies for staff retention, particularly in government institutions. Strengthening laboratories can establish a comprehensive national molecular testing system. Integrating molecular tests into health system diagnostic algorithms and implementing sustainable laboratory strategies will address human health challenges and support the “One Health” approach for animal and environmental health.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"111 3","pages":"Article 116716"},"PeriodicalIF":2.1,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surveillance of omadacycline against 35,000 bacterial clinical isolates from the United States (2019-2023)","authors":"Michael D. Huband ,&nbsp;Kelley A. Fedler ,&nbsp;Rodrigo E. Mendes ,&nbsp;Helio S. Sader ,&nbsp;Diane Anastasiou ,&nbsp;Alisa W. Serio ,&nbsp;Mariana Castanheira","doi":"10.1016/j.diagmicrobio.2025.116711","DOIUrl":"10.1016/j.diagmicrobio.2025.116711","url":null,"abstract":"<div><div>Omadacycline is an aminomethylcycline (tetracycline class) antibacterial approved by the US Food and Drug Administration in 2018 for the treatment of indicated organism groups and is available as intravenous and oral formulations. Omadacycline activity was assessed against 35,000 bacterial clinical isolates from medical centers in the United States (2019–2023) as part of the 5-year post-marketing surveillance commitment. Applying United States Food and Drug Administration breakpoint interpretive criteria, 98.2% of <em>Staphylococcus aureus</em> (including 96.1% of methicillin-resistant strains), 99.3% of <em>Staphylococcus lugdunensis</em>, 99.9% of <em>Streptococcus pneumoniae</em>, 99.4% of <em>Streptococcus pyogenes</em>, 99.9% of <em>Enterococcus faecalis</em>, 99.9% of <em>Haemophilus influenzae</em>, 92.4% of <em>Enterobacter cloacae</em>, and 92.5% of <em>Klebsiella pneumoniae</em> isolates were susceptible to omadacycline.</div><div>Omadacycline demonstrated consistent potent activity across the 5-year post-marketing surveillance program against key indicated organism groups and resistant organism subsets. These results demonstrate that omadacycline may merit further consideration for the treatment of Acute Bacterial Skin and Skin Structure Infections (ABSSSI) and Community Acquired Bacterial Pneumonia (CABP) as well as further study in the treatment of serious infections where resistant pathogens may be encountered, infections which lack currently available treatments, or where an oral treatment option is desired.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"111 3","pages":"Article 116711"},"PeriodicalIF":2.1,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cerebrospinal fluid multiplex PCR cycle thresholds may predict ICU admission in community-acquired meningoencephalitis","authors":"Pilar Vizcarra,&nbsp;David Grandioso Vas,&nbsp;María Inmaculada Quiles Melero,&nbsp;Juana Cacho Calvo,&nbsp;Emilio Cendejas Bueno","doi":"10.1016/j.diagmicrobio.2025.116704","DOIUrl":"10.1016/j.diagmicrobio.2025.116704","url":null,"abstract":"<div><h3>Objective</h3><div>We investigated the relationship between cerebrospinal fluid (CSF) PCR cycle threshold (Ct) values, clinical characteristics, and outcomes in suspected CAM, while assessing QIAstat-Dx® ME Panel concordance with routine diagnostic methods.</div></div><div><h3>Methods</h3><div>Frozen CSF from 30 individuals with suspected CAM were analyzed using the QIAstat-Dx® ME Panel. Ct values were categorized as low (≤ 30) or high (&gt;30). Concordance with CSF FilmArray®/culture results was assessed using the Kappa coefficient.</div></div><div><h3>Results</h3><div>Low Ct values were associated to CSF markers of meningitis and predicted ICU admissions (log-rank <em>P</em> = 0.025), particularly within the first two weeks of hospitalization (85 % for low Ct values versus 50 % for high Ct values, <em>P</em> = 0.041). Ct values were not associated with mortality in the survival analysis (log-rank <em>P</em> = 0.109). Substantial agreement was observed between QIAstat-Dx® and comparators (96.7 %, <em>Kappa</em> 0.839).</div></div><div><h3>Conclusions</h3><div>CSF Ct values can assist in risk stratification for timely ICU admission in individuals with CAM.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"111 3","pages":"Article 116704"},"PeriodicalIF":2.1,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors associated with the presence of Candida spp. in the oral and tracheobronchial secretions of patients admitted to an adult intensive care unit","authors":"Paulo Henrique da Cruz Ferreira ,&nbsp;Cristiane Rocha Fagundes Moura ,&nbsp;Vanessa Kelly da Silva Lage ,&nbsp;Leonardo Augusto da Costa Teixeira ,&nbsp;Henrique Silveira Costa ,&nbsp;Pedro Henrique Scheidt Figueiredo ,&nbsp;Joyce Noelly Vitor Santos ,&nbsp;Paulo Henrique da Cruz de Jesus ,&nbsp;Daniel Almeida Freitas ,&nbsp;Ana Cristina Rodrigues Lacerda ,&nbsp;Cíntia Lacerda Ramos ,&nbsp;Vanessa Amaral Mendonça","doi":"10.1016/j.diagmicrobio.2025.116687","DOIUrl":"10.1016/j.diagmicrobio.2025.116687","url":null,"abstract":"<div><div>objective: to verify factors associated with the presence of Candida spp. in oral and tracheobronchial secretions of 135 patients admitted to an adult ICU, general of the Santa Casa de Caridade, of Diamantina, Minas Gerais, Brazil. Oral samples showed significant differences between colonized and non-colonized patients in the variables age (<em>p</em> &lt; 0.01), people with two or more comorbidities (<em>p</em> &lt; 0.04), hypertensive patients (<em>p</em> &lt; 0.01) and Congestive Heart Failure (CHF) (<em>p</em> &lt; 0.01). In multivariate logistic regression, we verified that age (<em>p</em> = 0.02, OR = 1.03), Systemic Arterial Hypertension (SAH) (<em>p</em> = 0.01, OR = 2.89) and CHF (<em>p</em> = 0.05, OR = 2.49) are associated with the presence of Candida spp. in oral samples. Removing the age variable, comorbid hypertension (<em>p</em> = 0.04, OR = 2.18) and CHF (<em>p</em> = 0.04, OR = 2.64) increase the chance of having Candida spp. twice in the oral sample. The results showed that CHF, SAH and increasing age are factors associated with Candida spp. in the oral cavity of ICU patients.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"111 3","pages":"Article 116687"},"PeriodicalIF":2.1,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143058194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Testicular toxoplasmosis: An exceptionally rare manifestation of human toxoplasmosis","authors":"Edmond Puca ,&nbsp;Entela Puca ,&nbsp;Faruk Dogan ,&nbsp;Dea Puca","doi":"10.1016/j.diagmicrobio.2025.116709","DOIUrl":"10.1016/j.diagmicrobio.2025.116709","url":null,"abstract":"<div><div>Toxoplasmosis, caused by the intracellular parasite <em>Toxoplasma gondii</em>, is a globally prevalent infection. The clinical manifestations are often asymptomatic, particularly in immunocompetent patients. Human testicular toxoplasmosis is an extremely rare presentation occasionally observed in immunocompromised patients. This case report presents a 32-year-old immunocompetent male with a history of right radical orchiectomy for a mixed germ cell tumor who presented with acute left testicular pain and was diagnosed with testicular toxoplasmosis.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"111 3","pages":"Article 116709"},"PeriodicalIF":2.1,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genotyping and antimicrobial resistance profile of ESKAPE pathogens from a tertiary care hospital in Jammu & Kashmir, India","authors":"Sabia Qureshi ,&nbsp;Faheem u Din ,&nbsp;Muddasir S Banday ,&nbsp;Anjum Farhana ,&nbsp;Nahid Nehvi ,&nbsp;Zahid Kashoo ,&nbsp;Leenah Bashir ,&nbsp;Mir Nadeem Hassan ,&nbsp;Gulzar Badroo ,&nbsp;M. Altaf Bhat ,&nbsp;Mansoor Nabi ,&nbsp;Shaheen Farooq ,&nbsp;Md. Isfaqul Hussain ,&nbsp;Pervaiz Dar ,&nbsp;Maliha Gulzar ,&nbsp;Junaid Mehraj","doi":"10.1016/j.diagmicrobio.2025.116714","DOIUrl":"10.1016/j.diagmicrobio.2025.116714","url":null,"abstract":"<div><div>The rise of MDR (multi drug resistant) ESKAPE pathogens in hospitals is a pressing global healthcare crisis, demanding urgent attention and action. The current study investigates the prevalence and antimicrobial resistance patterns of ESKAPE pathogens in a tertiary care hospital in the union territory of Jammu and Kashmir, India. Out of 244 ESKAPE pathogens isolated in present study from patients of a tertiary care hospital, 84 (34.4 %) isolates were identified as <em>Staphylococcus aureus</em>, of which 59.52 % were identified as methicillin resistant <em>S. aureus</em> (MRSA), 4.76 % were methicillin sensitive <em>S. aureus</em> (MSSA) and 35.71 % were methicillin resistant coagulase negative staphylococci (MR-CoNS). The <em>mec</em>A gene (methicillin resistant staphylococcal cassette chromosome) was detected in 61.9 % of the <em>S. aureus</em> isolates of the present study. <em>Klebsiella pneumoniae</em> was the second most prevalent (28.75 %) pathogen, followed by <em>Pseudomonas aeruginosa</em> and <em>Escherichia coli</em> (27.5 % each), <em>Acinetobacter baumannii</em> (11.87 %), and others. A higher number of isolates were recovered from male patients (61 %) than from female patients (39 %). Screening for β-lactamase genes (<em>bla</em>_TEM, <em>bla</em>_SHV, <em>bla</em>_CTX-M-15) revealed that 32.5 % of isolates carried the TEM gene (Temoniera), 36.87 % had the CTX-M-15 gene (CefoTaXimase-Munich), and 20.62 % harboured SHV variants (Sulfhydryl Variable). Notably, 23 isolates, predominantly <em>K. pneumoniae</em>, were positive for all three β-lactamase genes, highlighting the significant prevalence of multidrug resistance among ESKAPE pathogens in the region. This study provides epidemiological data on the prevalence of ESKAPE isolates in tertiary care hospital in the union territory of Jammu and Kashmir, India. To our knowledge, this is the first comprehensive report on the prevalence of ESBL genes in ESKAPE pathogens of this region. Further, this study underscores the critical need for enhanced surveillance and targeted antimicrobial stewardship programs to address the growing challenge of antimicrobial resistance in hospital settings.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"111 3","pages":"Article 116714"},"PeriodicalIF":2.1,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143122404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retrospective analysis and clinical performance of BD MAX enteric pathogen testing with a focus on reliable identification of vibrio species in stool samples","authors":"Claire N. Freeman ,&nbsp;Ninad Mehta ,&nbsp;Jay Rabari ,&nbsp;Justin Kosar ,&nbsp;Joseph M. Blondeau ,&nbsp;Camille L. Hamula","doi":"10.1016/j.diagmicrobio.2025.116715","DOIUrl":"10.1016/j.diagmicrobio.2025.116715","url":null,"abstract":"<div><div>Infectious diarrhea is a significant health concern, particularly for immunocompromised and pediatric patients. Traditional stool culture methods are labor-intensive and often lack sensitivity, prompting clinical laboratories to adopt PCR-based gastrointestinal pathogen panels (GIPPs). This study evaluated the BD MAX™ GIPP compared to traditional methods, focusing on the accuracy of the platform's automatic interpretations. Positivity rates increased from 4.0 % to 6.4 % after implementing BD MAX. The system detected significantly more <em>Vibrio</em> spp. and <em>Yersinia enterocolitica</em> than traditional culture, with most of these positives unrecoverable by culture. Irregular PCR amplification curves were frequently observed in cases where viable organisms were not recovered in culture, yet these still produced positive automatic interpretations. These findings emphasize the need for cautious interpretation of results and highlight the importance of incorporating amplification curve reviews into laboratory workflows to improve accuracy and ensure reliable diagnoses.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"111 4","pages":"Article 116715"},"PeriodicalIF":2.1,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143350406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of cavity absorption on treatment outcomes of Mycobacterium kansasii pulmonary disease","authors":"Xin Zou ,&nbsp;Shulin Yi ,&nbsp;Mengxing Luo ,&nbsp;Qibing Zeng ,&nbsp;Shixiu Yao ,&nbsp;Lulu Chen ,&nbsp;Xin Yao ,&nbsp;Yang Hu ,&nbsp;Zhonghua Liu","doi":"10.1016/j.diagmicrobio.2025.116712","DOIUrl":"10.1016/j.diagmicrobio.2025.116712","url":null,"abstract":"<div><h3>Background</h3><div>The incidence of <em>Mycobacterium kansasii</em> pulmonary disease (MK-PD) was increasing worldwide. However, limited research was conducted on how to improve the cure rate and evaluate treatment efficacy for patients. We aimed to identify key factors that could improve the cure rate in patients with MK-PD.</div></div><div><h3>Methods</h3><div>In this retrospective cohort study, the medical records of patients with MK-PD, registered with Shanghai Pulmonary Hospital between January 2016 and December 2020, were collected. These included data on demographics, as well as clinical, radiological, microbiological, drug resistance, and drug regimen information, and treatment outcomes. Multivariate logistic regression analysis was conducted to identify risk factors for treatment failure in patients.</div></div><div><h3>Results</h3><div>This study included 129 patients, of whom 100 (77.5 %) were treated successfully. Logistic regression analysis identified non-absorption of pulmonary cavities as an independent risk factor for treatment successful (odds ratio: 7.9, 95 %confidence interval: 1.7–36.2, <em>p</em> = 0.008). The overall cure rate for patients with absorption of pulmonary cavities (APC) was 93 %; 55 % of patients began resorption within 6 months of the start of treatment (the cure rate was 95 %). The APC rate of patients with MK-PD negatively correlated with the number of comorbidities (<em>p</em> = 0.009); that was, the more comorbidities, the more difficult the cavity absorption and the lower the cure rate.</div></div><div><h3>Conclusions</h3><div>The absorption of pulmonary cavities was the key to the cure rate for patients with MK-PD. Early changes in cavities helped identify the effect of treatment and guide doctors in optimizing treatment plans to improve patient cure rates.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"111 4","pages":"Article 116712"},"PeriodicalIF":2.1,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143284137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decoding omicron: Genetic insight into its transmission dynamics, severity spectrum and ever-evolving strategies of immune escape in comparison with other SARS-CoV-2 variants","authors":"Kundan Tandel ,&nbsp;Divya Niveditha ,&nbsp;Sanjay Pratap Singh ,&nbsp;Kavita Bala Anand ,&nbsp;Vaishnavi Shinde ,&nbsp;Mayank Ghedia ,&nbsp;Ashwini Sondakar ,&nbsp;Mahesh Reddy","doi":"10.1016/j.diagmicrobio.2025.116705","DOIUrl":"10.1016/j.diagmicrobio.2025.116705","url":null,"abstract":"<div><h3>Background</h3><div>The coronavirus disease 2019 (COVID-19) pandemic, driven by the rapid evolution of the SARS-CoV-2 virus, has led to the emergence of multiple variants with significant impacts on global health. This study aims to analyze the evolutionary trends and mutational landscape of SARS-CoV-2 variants circulating in Pune, Maharashtra, India, from August 2022 to April 2024. Using comprehensive genomic surveillance data, we identified the predominance of variants such as BA.2.75, XBB.x, and the newly emerged subvariants JN.1, KP.1, and KP.2. These subvariants, belonging to the BA.2.86 lineage, have raised concerns owing to their potential for increased transmissibility and immune evasion.</div></div><div><h3>Results</h3><div>Phylogenetic analysis of 84 sequenced samples from Pune revealed 18 distinct lineages, with JN.1 and KP.2 forming a novel branch compared with their ancestral lineage, BA.2. Detailed mutational analysis highlighted key mutations in the N-terminal domain (NTD) and receptor-binding domain (RBD) of the spike protein, affecting viral stability, ACE2 binding affinity, and neutralizing antibody escape. Our findings, along with the predictions of SpikePro, suggest that the combination of these mutations enhances the viral fitness of JN.1 and KP.2, contributing to their rapid emergence and spread.</div></div><div><h3>Conclusion</h3><div>This study underscores the importance of continuous genomic surveillance and advanced computational modeling to track and predict the evolutionary trajectories of SARS-CoV-2 variants. The insights gained from this research are crucial for informing public health strategies, vaccine updates, and therapeutic interventions to mitigate the impact of current and future SARS-CoV-2 variants.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"111 3","pages":"Article 116705"},"PeriodicalIF":2.1,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic utility of next-generation sequencing in neurological infections: Two cases in point","authors":"Ravi Teja Kandikuppa ,&nbsp;Vidya Krishna ,&nbsp;Nandini Sethuraman ,&nbsp;Anuradha Sridhar ,&nbsp;Srinivasan Paramasivam","doi":"10.1016/j.diagmicrobio.2025.116713","DOIUrl":"10.1016/j.diagmicrobio.2025.116713","url":null,"abstract":"<div><div>We report the successful management of two cases of brain abscess and subdural empyema caused by <em>Fusobacterium nucleatum</em> and <em>Streptococcus intermedius</em> diagnosed using 16S ribosomal RNA (rRNA) sequencing where microbiological culture showed no growth. This report elucidates the utility of next-generation sequencing (NGS) in determining the microbial etiology and guiding appropriate antimicrobial treatment in life-threatening neurological infections.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"111 3","pages":"Article 116713"},"PeriodicalIF":2.1,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}