Diagnostic microbiology and infectious disease最新文献

{"title":"Incidence and antimicrobial resistance profile of multi-drug resistant Salmonella typhi isolated from blood samples in Khyber Pakhtunkhwa, Pakistan","authors":"Jawad Ahmad ,&nbsp;Muhammad Ahmar Khan ,&nbsp;Muhammad Atif ,&nbsp;Zia Ur Rahman ,&nbsp;Ayesha Nasir ,&nbsp;Hafiza Misbah Ahmad ,&nbsp;Ayesha Haroon ,&nbsp;Azeem Sarwar ,&nbsp;Muhammad Afrasiab Khan ,&nbsp;Numan Fazal","doi":"10.1016/j.diagmicrobio.2025.117122","DOIUrl":"10.1016/j.diagmicrobio.2025.117122","url":null,"abstract":"<div><div><em>Salmonella typhi</em> has shown increasing resistance to antibiotics in recent years, creating serious challenges in treatment. Inappropriate use of antibiotics without testing bacterial sensitivity often results in therapeutic failure, highlighting the importance of bacterial isolation and susceptibility testing. A study was carried out from July 2022 to July 2023 to assess the incidence of multidrug-resistant (MDR) <em>S. typhi</em> in clinical samples and evaluate its susceptibility profile across different districts of Khyber Pakhtunkhwa, Pakistan.</div><div>A total of 1,625 blood samples were collected from patients with suspected enteric fever in Peshawar, Dir, Mardan, and Swat. Of these, 341 isolates (20.98 %) were confirmed as <em>S. typhi</em> using biochemical tests and PCR amplification of the fliC gene. Infection was more common in males (57.7 %), while children ≤10 years were most affected (54.25 %). Seasonal distribution revealed highest prevalence in summer (38.41 %). Among districts, Peshawar reported the highest occurrence (78.29 %), followed by Dir (14.36 %) and Mardan (7.03 %). A significant correlation was found between districts and infection rate, though age and gender showed no significant association.</div><div>Antimicrobial testing revealed high susceptibility to meropenem (90.71 %) and tetracycline (71.83 %), but marked resistance to ampicillin (77.40 %), co-trimoxazole (74.61 %), ceftriaxone (73.68 %), and erythromycin (71.52 %). Notably, 71.94 % isolates were MDR and 22.38 % were extensively drug-resistant (XDR). PCR detection confirmed high prevalence of resistance genes, particularly blaTEM (61 %), sul2 (76 %), and sul1 (57 %).</div><div>This study underscores the alarming rise of MDR <em>S. typhi</em> in Khyber Pakhtunkhwa and stresses the urgent need for effective surveillance, prevention, and antimicrobial stewardship.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 2","pages":"Article 117122"},"PeriodicalIF":1.8,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145227680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Significance of the avidity test in IgM+/IgG+ patients infected with Toxoplasma gondii and cytomegalovirus and its relationship with demographic data","authors":"Pınar Etiz ,&nbsp;Toygar Emre ,&nbsp;Salih Çetiner ,&nbsp;Adil M. Allahverdiyev","doi":"10.1016/j.diagmicrobio.2025.117126","DOIUrl":"10.1016/j.diagmicrobio.2025.117126","url":null,"abstract":"<div><h3>Background</h3><div>IgG avidity testing is a valuable tool in distinguishing recent from past infections. This study presents a large-scale evaluation of IgG avidity test results for <em>Toxoplasma gondii</em> (toxoplasmosis) and Cytomegalovirus infections among IgM+/IgG+ patients, exploring its relationship with demographic factors with in a tertiary hospital.</div></div><div><h3>Methods</h3><div>Toxoplasma and CMV IgG/IgM levels were measured using the chemiluminescent microparticle immunoassay method. IgG avidity was assessed in IgM+/IgG+ serum samples using Micro ELISA System for <em>Toxoplasma gondii</em> and electrochemiluminescence immunoassay method for Cytomegalovirus. Avidity indices were analyzed in relation to demographic data using SPSS software.</div></div><div><h3>Results</h3><div>Between 2017 and 2024, 15,034 CMV-IgG, 20,090 CMV-IgM, and 5,693 CMV-IgG avidity tests were performed. Among 212 CMV cases analyzed for avidity (mean age 25.89 ± 26.13 years, 54.2 % male), 69.8 % had high avidity and 17.9 % had low avidity. Similarly, 14,937 Toxo-IgG, 19,895 Toxo-IgM and 4,623 Toxo-IgG avidity tests were performed. Of the 180 cases analyzed for avidity (mean age 30.01 ± 14.66 years, 85.0 % female); 70.0 % had high avidity and 15.6 % had low avidity.</div></div><div><h3>Conclusion</h3><div>In suspected Toxoplasma and CMV infections, combined IgG/IgM testing followed by IgG avidity testing is crucial for determining infection timing. Collaboration between clinicians and laboratory specialists is vital for accurate diagnosis and management, considering demographic factors.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 2","pages":"Article 117126"},"PeriodicalIF":1.8,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145227329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The invisible burden of pediatric tuberculosis in sub-Saharan Africa: An urgent call for child-centered diagnostic strategies","authors":"Hermann Yokolo","doi":"10.1016/j.diagmicrobio.2025.117127","DOIUrl":"10.1016/j.diagmicrobio.2025.117127","url":null,"abstract":"","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 2","pages":"Article 117127"},"PeriodicalIF":1.8,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cover 2 - Aims/Scopes, Ed Board","authors":"","doi":"10.1016/S0732-8893(25)00436-5","DOIUrl":"10.1016/S0732-8893(25)00436-5","url":null,"abstract":"","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 1","pages":"Article 117114"},"PeriodicalIF":1.8,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pseudomonas guariconensis Necrotizing soft tissue infection, Denmark","authors":"Sofus Rønne Soltau ,&nbsp;Nina Elisabeth Pontoppidan Monrad ,&nbsp;Allan Evald Væver Nielsen ,&nbsp;Jacob Ehlers-Hansen ,&nbsp;Henrik Pierre Calum","doi":"10.1016/j.diagmicrobio.2025.117121","DOIUrl":"10.1016/j.diagmicrobio.2025.117121","url":null,"abstract":"<div><div>We describe the first published case report of a successful treatment of <em>Pseudomonas guariconensis</em>-induced Necrotizing Soft Tissue Infection (NSTI). Since <em>Pseudomonas guariconensis</em> has been resistant to meropenem in all reported cases, clinicians and microbiologists should treat these patients with extreme care.</div></div><div><h3>Patient and methods</h3><div>A 72-year-old man presented to the emergency department with a 3-day history of fever, pain in the right leg/foot and general malaise. He was diagnosed with NSTI, empirical meropenem and clindamycin was started, and surgery was quickly performed with removal of all dead skin and subcutaneous tissue. All fasciae were intact.</div></div><div><h3>Results</h3><div>Blood cultures and tissue samples showed growth of <em>Pseudomonas guariconensis</em>, which a literature review revealed to be highly suspicious of meropenem resistance. This prompted quick change of antibiotics, first with ciprofloxacin and metronidazole added and then change of meropenem to cefiderocol. Susceptibility tests on both blood and tissue samples revealed resistance to meropenem (along with resistance to many other antibiotics). The patient improved, was changed to ceftazidim and ciprofloxacin and recovered fully from the infection.</div></div><div><h3>Conclusions</h3><div>Since clindamycin has no effect on any <em>Pseudomonas species</em> and since <em>Pseudomonas guariconensis</em> has been resistant to meropenem in all reported cases, clinicians and microbiologists should treat these patients with extreme care.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 2","pages":"Article 117121"},"PeriodicalIF":1.8,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145227328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pet-human transmission of CTX-M-8 extended-spectrum β-lactamase-producing Escherichia coli ST961 and phylogenetic group B2","authors":"Luciana Sartori ,&nbsp;Fábio P. Sellera ,&nbsp;João Pedro Rueda Furlan ,&nbsp;Quézia Moura ,&nbsp;Terezinha Knöbl ,&nbsp;Nilton Lincopan","doi":"10.1016/j.diagmicrobio.2025.117120","DOIUrl":"10.1016/j.diagmicrobio.2025.117120","url":null,"abstract":"<div><div>Extended-spectrum β-lactamase (ESBL)-producing <em>Escherichia coli</em> pose a One Health concern. We report potential transmission of a CTX-M-8-producing <em>E. coli</em> ST961 strain between a French Bulldog and its owner. Both isolates showed similar antimicrobial resistance and virulence profiles, with only 30 SNP differences, highlighting zoonotic transmission risk and integrated health strategies.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 2","pages":"Article 117120"},"PeriodicalIF":1.8,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145185046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of colistin susceptibility among Klebsiella pneumoniae isolates by broth microdilution and colistin agar test","authors":"Hitha Haridas ,&nbsp;Shweta Raina ,&nbsp;Priyanka Das,&nbsp;Shweta Kunwar,&nbsp;Rajni Gaind","doi":"10.1016/j.diagmicrobio.2025.117119","DOIUrl":"10.1016/j.diagmicrobio.2025.117119","url":null,"abstract":"<div><h3>Background</h3><div>Colistin is a last-resort antibiotic against multidrug-resistant (MDR) <em>Klebsiella pneumoniae</em>. Reliable susceptibility testing is critical but challenging due to colistin’s complex pharmacodynamics and testing limitations. This study evaluates the performance of the Colistin Agar Test (CAT) against the gold standard Broth Microdilution (BMD) method.</div></div><div><h3>Methods</h3><div>Study was conducted after confirming stability of colistin in agar plate. A total of 150 non-duplicate clinical isolates of <em>K. pneumoniae</em> (103 colistin intermediate, 47 resistant) were tested using both BMD and CAT. Antimicrobial susceptibility testing (AST) of these isolates was performed using VITEK-2. Quality control was ensured using CLSI-recommended strains. Statistical parameters including Categorical Agreement (CA), Essential Agreement (EA), Major Error (ME), Very Major Error (VME), sensitivity, specificity, predictive values, and Cohen’s Kappa were calculated using BMD as reference.</div></div><div><h3>Results</h3><div>MICs ranged from 0.125-16 µg/mL (BMD) and 0.125–8 µg/mL (CAT). CA and EA for CAT were 96 % and 87.3 % respectively. ME and VME were 4.8 % and 2.1 %. Sensitivity, specificity, PPV, and NPV of CAT were 97.9 %, 95.1 %, 90.1 %, and 98.9 % respectively. Cohen’s Kappa showed almost perfect agreement (0.91).</div></div><div><h3>Conclusion</h3><div>CAT demonstrated high agreement with BMD and showed promise as a screening tool for colistin susceptibility in <em>K. pneumoniae</em>. While BMD remains the gold standard, CAT offers a feasible alternative for routine use, particularly in resource-limited settings. However, resistant results by CAT should be confirmed with BMD to avoid false resistance reporting and potential therapeutic exclusion of colistin.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 2","pages":"Article 117119"},"PeriodicalIF":1.8,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145156581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anaerobic breast abscess caused by Prevotella bivia in non-lactating woman: A case report with diagnostic and clinical implications","authors":"Chiara Massaro ,&nbsp;Cinzia Cala ,&nbsp;Antonio Anastasia ,&nbsp;Simona Lupo ,&nbsp;Rosa Alduina ,&nbsp;Teresa Fasciana ,&nbsp;Roberta Virruso ,&nbsp;Giovanni M. Giammanco","doi":"10.1016/j.diagmicrobio.2025.117112","DOIUrl":"10.1016/j.diagmicrobio.2025.117112","url":null,"abstract":"<div><h3>Background and Clinical Significance</h3><div><em>Prevotella bivia</em> is an anaerobic Gram-negative bacillus commonly associated with pelvic and genital tract infections. Its isolation from breast abscesses, particularly in nonpuerperal women, is extremely uncommon.</div></div><div><h3>Case presentation</h3><div>We report a unique case of a 40-year-old non- lactating woman presenting with a recurrent right breast abscess. The pa- tient experienced three episodes over three years (2022–2025); only the fourth episode was microbiologically confirmed as being caused by <em>P. bivia</em>, identi- fied using MALDI-TOF MS. Antimicrobial susceptibility testing revealed resistance to ampicillin and susceptibility to amoxicillin/clavulanic acid and other agents.</div></div><div><h3>Conclusion</h3><div>The case underlines the critical role of anaerobic cultures and advanced identification techniques in recurrent breast infections. Although microbiological confirmation was achieved only in the latest episode, the recurrent clinical picture suggests a difficult-to-culture microbial aetiology.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 2","pages":"Article 117112"},"PeriodicalIF":1.8,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145102610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiological and clinical impact of aminoglycosides on KPC-producing Klebsiella pneumoniae bacteraemia with aminoglycoside-modifying enzymes","authors":"Jae Eun Seong ,&nbsp;Sang Min Ahn ,&nbsp;Min Han ,&nbsp;Yong Seop Lee ,&nbsp;Jung Ah Lee ,&nbsp;Jae Hoon Kim ,&nbsp;Jung Ho Kim ,&nbsp;Jin Young Ahn ,&nbsp;Su Jin Jeong ,&nbsp;Nam Su Ku ,&nbsp;Joon Sup Yeom ,&nbsp;Hyukmin Lee ,&nbsp;Jun Yong Choi","doi":"10.1016/j.diagmicrobio.2025.117111","DOIUrl":"10.1016/j.diagmicrobio.2025.117111","url":null,"abstract":"<div><h3>Background</h3><div>Klebsiella pneumoniae (KPN) is a major pathogen associated with life-threatening infections. The global rise in carbapenem-resistant KPN, particularly <em>Klebsiella pneumoniae</em> carbapenemase (KPC)-producing strains, presents a treatment challenge. The efficacy of aminoglycosides against KPC-producing KPN strains carrying aminoglycoside-modifying enzyme (AME) genes remains unclear. This study evaluated the clinical and microbiological effects of aminoglycoside therapy on AME gene-bearing KPC-producing KPN bacteraemia.</div></div><div><h3>Methods</h3><div>We conducted a retrospective cohort study on adults with amikacin-susceptible KPC-producing KPN bacteraemia at a South Korean tertiary hospital (2022–2024). Antimicrobial susceptibility was assessed using VITEK 2 and disc diffusion per Clinical and Laboratory Standards Institute guidelines. The <em>bla</em>_KPC gene was detected via immunochromatography, whereas whole-genome sequencing identified <em>bla</em>_KPC subtypes and AME genes. Clinical and microbiological outcomes were analysed based on aminoglycoside use.</div></div><div><h3>Results</h3><div>Among 136 patients (mean age: 63.6 years), 98 received aminoglycoside therapy, and 38 did not. Intra-abdominal infections were the most common source. Microbiological clearance within one week was significantly lower in the aminoglycoside group (63.3 % vs. 84.2 %, (<em>p</em> = 0.005). Clinical improvement within three days was also lower (48 % vs. 73.7 %). Mortality rates (28-d: 26.5 % vs. 21.1 %, <em>p</em> = 0.660; in-hospital: 40.8 % vs. 34.2 %, <em>p</em> = 0.559) did not differ significantly. Multivariable analysis found no significant association between aminoglycoside use and 28-d mortality (odds ratio 0.99; 95 % confidence interval: 0.33–3.13, <em>p</em> = 0.988).</div></div><div><h3>Conclusion</h3><div>Aminoglycoside therapy did not improve clinical or microbiological outcomes in AME gene-bearing KPC-producing KPN bacteraemia, despite phenotypic susceptibility.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 1","pages":"Article 117111"},"PeriodicalIF":1.8,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145079922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High in vitro activity of Fosfomycin in carbapenemase-producing Klebsiella pneumoniae and carbapenem-resistant Pseudomonas aeruginosa in clinical isolates in Colombia","authors":"Elsa De La Cadena ,&nbsp;German Esparza ,&nbsp;Juan Carlos García-Betancur ,&nbsp;Natalia Restrepo-Arbeláez ,&nbsp;Betsy E. Castro ,&nbsp;Christian José Pallares ,&nbsp;María Virginia Villegas","doi":"10.1016/j.diagmicrobio.2025.117110","DOIUrl":"10.1016/j.diagmicrobio.2025.117110","url":null,"abstract":"<div><h3>Objectives</h3><div>We report the fosfomycin resistance rates in clinical isolates of carbapenemase-producing <em>K. pneumoniae</em> (CPKP) and carbapenem-resistant <em>P. aeruginosa</em> (CRPA) in Colombia, as well as the characterization of mechanisms of resistance to fosfomycin.</div></div><div><h3>Methods</h3><div>CPKP (<em>n</em> = 130) and CRPA (<em>n</em> = 170) isolates were collected from 12 Colombian hospitals between 2018 and 2022. Fosfomycin susceptibility testing was performed by agar dilution. Analysis of the MurA, GlpT, UhpT, UhpA, PtsI and CyaA were performed in all fosfomycin-resistant isolates using WGS.</div></div><div><h3>Results</h3><div>Minimal inhibitory concentrations below the ECOFFs were detected in 97,8 % isolates. Among the 5 fosfomycin-resistant CPKP, all isolates harbored a mutation in the MurA protein (Tyr287Asn) and a substitution (Gly171Arg) in the UhpT. 60 % fosfomycin-resistant CPKP had the Ile91Val mutation associated with the FosA^KPN, one isolate showed a substitution in the GlpT protein (Tyr141Cys), and one isolate displayed a premature stop codon of the GlpT (ES86). No mutations were found in GlpR, PtsI, or CyaA in CPKP resistant to fosfomycin. One fosfomycin-resistant CRPA isolate was identified, showing a deletion of 6 bp in <em>glpT</em>, as well as other mutation in the same gene, leading to the Gly422Ser substitution.</div></div><div><h3>Conclusions</h3><div>for CRPA and particularly, for CPKP resistant to fosfomycin, there is a diversity in their resistance mechanisms with mutations in the MurA as the prevalent mechanism in CPKP. Also, FosA^KPN and GlpT mutations were identified as the other mechanisms of fosfomycin resistance in these clinical isolates. Fosfomycin can be considered a therapeutic option in combination therapy to treat CPKP and CRPA in Colombia.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 1","pages":"Article 117110"},"PeriodicalIF":1.8,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145091364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}