Diagnostic microbiology and infectious disease最新文献_第2页

Establishment of a microfluidic and RPA-based platform for rapid multi-sample detection of Mycoplasma pneumoniae

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-04-10 DOI: 10.1016/j.diagmicrobio.2025.116840

Ling Zhang , Cheng-Di Sun , Jiao Luo , Li-Guo Liang , Si-Ming Lu

{"title":"Establishment of a microfluidic and RPA-based platform for rapid multi-sample detection of Mycoplasma pneumoniae","authors":"Ling Zhang , Cheng-Di Sun , Jiao Luo , Li-Guo Liang , Si-Ming Lu","doi":"10.1016/j.diagmicrobio.2025.116840","DOIUrl":"10.1016/j.diagmicrobio.2025.116840","url":null,"abstract":"<div><h3>Background</h3><div><em>Mycoplasma pneumoniae</em> (<em>M. pneumoniae</em>) is a major respiratory pathogen causing atypical community-acquired pneumonia. Early and rapid diagnosis and timely detection of pathogens are crucial. RPA is an emerging PCR technology that allows amplification at room temperature compared to conventional PCR technology, while combining the advantages of rapid detection and high specificity of PCR technology, enabling rapid and accurate detection of pathogens.</div></div><div><h3>Methods and results</h3><div>In this study, we established a platform for detection <em>of M. pneumoniae</em> based on microfluidic and RPA technologies. The RPA technology is performed in 15 to 20 minutes, and only <em>M. pneumoniae</em> is significantly amplified at a sensitivity of 10 copies/μL; microfluidic technology provides lean instrumentation and a convenient interactive system. Three groups negative and positive QCs and 18 clinical samples can be tested in a single run.</div></div><div><h3>Significance</h3><div>In conclusion, we have established a microfluidic molecular assay operating system based on RPA technology, with the advantages of being simple, rapid, accurate and economical, and providing a new tool for laboratory detection of <em>M. pneumoniae</em>.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 4","pages":"Article 116840"},"PeriodicalIF":2.1,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Successfully treated myopericarditis and acute heart failure due to severe Neisseria meningitidis infection: a case report

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-04-08 DOI: 10.1016/j.diagmicrobio.2025.116837

Nikolaos Nektarios Karamanolis , Christos G Nikolaidis , Ioannis Gavgiotakis , Aikaterini Gaki , Iraklis Tatsis , Alexandra Mika , George D Liatsos , Dimitrios Vassilopoulos

引用次数: 0

Development and evaluation of ELISA serological immunoassays for influenza and respiratory syncytial viruses

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-04-07 DOI: 10.1016/j.diagmicrobio.2025.116835

Alexandra Claudet , Noémi Alcover , Elise Lebigot , Hana-Sofia Bouhelal , Fairly Warnakulasuriya , Marie-Pierre Soutiere , Marie Galloux , Jean-François Eleouet , Marie-Anne Rameix-Welti , Etienne Bizot , Christelle Vauloup-Fellous , Vincent Portet-Sulla

{"title":"Development and evaluation of ELISA serological immunoassays for influenza and respiratory syncytial viruses","authors":"Alexandra Claudet , Noémi Alcover , Elise Lebigot , Hana-Sofia Bouhelal , Fairly Warnakulasuriya , Marie-Pierre Soutiere , Marie Galloux , Jean-François Eleouet , Marie-Anne Rameix-Welti , Etienne Bizot , Christelle Vauloup-Fellous , Vincent Portet-Sulla","doi":"10.1016/j.diagmicrobio.2025.116835","DOIUrl":"10.1016/j.diagmicrobio.2025.116835","url":null,"abstract":"<div><h3>Introduction</h3><div>Respiratory syncytial virus (RSV) and influenza are major causes of respiratory infections globally. Although vaccines are available, serological tools to assess population-level immunity and maternal antibody transfer remain limited. This study aimed to develop and evaluate ELISA assays for detecting anti-RSV and anti-influenza antibodies as a basis for future maternal-fetal immunity research.</div></div><div><h3>Materials and Methods</h3><div>In this study, we designed ELISA immunoassays using various influenza and RSV antigens. Sensitivity and specificity were calculated using sera from presumed seronegative infants and seropositive adults.</div></div><div><h3>Results</h3><div>The influenza vaccine-based ELISA achieved 98.3 % sensitivity and 100 % specificity. The pre-fusion F protein of RSV (Arexvy®) showed 97.5 % sensitivity and 97.4 % specificity. Other antigen combinations performed less optimally.</div></div><div><h3>Discussion</h3><div>These ELISA assays are scalable tools for seroepidemiological and maternal transfer studies. Future work will include correlation with neutralizing antibodies and paired maternal-infant analyses.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 3","pages":"Article 116835"},"PeriodicalIF":2.1,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143816261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Description of blaKPC-carrying Escherichia coli in patients from a Brazilian hospital over a 4-year period

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-04-05 DOI: 10.1016/j.diagmicrobio.2025.116833

Letícia Kalir Pradela , Tiago Casella , Fernanda Zani Manieri , Letícia Kellen de Andrade , Marlon do Valle Barroso , Nguyen Thi Khanh Nhu , Mark Andrew Schembri , Cristiano Gallina Moreira , Mara Corrêa Lelles Nogueira

{"title":"Description of blaKPC-carrying Escherichia coli in patients from a Brazilian hospital over a 4-year period","authors":"Letícia Kalir Pradela , Tiago Casella , Fernanda Zani Manieri , Letícia Kellen de Andrade , Marlon do Valle Barroso , Nguyen Thi Khanh Nhu , Mark Andrew Schembri , Cristiano Gallina Moreira , Mara Corrêa Lelles Nogueira","doi":"10.1016/j.diagmicrobio.2025.116833","DOIUrl":"10.1016/j.diagmicrobio.2025.116833","url":null,"abstract":"<div><div><em>Klebsiella pneumoniae</em> carbapenemase (KPC)-producing <em>Escherichia coli</em> are recognized by the World Health Organization as a critical group of bacterial priority pathogens of public health importance. Thus, increased understanding of the genetic characteristics of KPC-producing <em>E. coli</em> is required. Here, we performed a retrospective study in a Brazilian teaching-hospital to describe the genomic features linked to antimicrobial resistance, virulence, and phylogeny of 40 meropenem-resistant <em>E. coli</em>. All isolates carried the <em>bla</em><sub>KPC-2</sub> gene, but amikacin, tigecycline, colistin, polymyxin B, and fosfomycin showed good activity. Molecular typing by MLST revealed 20 sequence types (STs), with a predominance of ST131. Whole-genome sequencing identified Tn<em>4401</em> as a mechanism responsible for <em>bla</em><sub>KPC-2</sub> mobilization, a variety of antimicrobial resistance and virulence genes, the predominance of pathogenic phylogroup lineages, and the grouping of genomes belonging to the same ST. KPC-producing <em>E. coli</em> is not a common pathogen, but few treatment alternatives are available against potentially virulent strains.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 3","pages":"Article 116833"},"PeriodicalIF":2.1,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143807613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of humoral and cellular responses after vaccination against SARS-CoV-2 in patients with immune-mediated diseases

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-04-04 DOI: 10.1016/j.diagmicrobio.2025.116825

Thomas Escoda , Sylvie Jordana , Laurent Chiche , Guillaume Penaranda , Stanislas Rebaudet , Philippe Halfon

{"title":"Analysis of humoral and cellular responses after vaccination against SARS-CoV-2 in patients with immune-mediated diseases","authors":"Thomas Escoda , Sylvie Jordana , Laurent Chiche , Guillaume Penaranda , Stanislas Rebaudet , Philippe Halfon","doi":"10.1016/j.diagmicrobio.2025.116825","DOIUrl":"10.1016/j.diagmicrobio.2025.116825","url":null,"abstract":"<div><h3>Background</h3><div>Patients with autoimmune disease (AID) or immunodepression (ID), particularly those treated with anti-CD20, have an increased risk of COVID-19 infection.</div></div><div><h3>Objective</h3><div>To characterise the humoral and cellular immune responses against specific antigens of SARS-CoV-2 in immunocompromised patients, as well as their correlation and determinants.</div></div><div><h3>Methods</h3><div>This retrospective study was conducted in outpatients with AID and/or ID for which an assessment of their humoral and cellular response was carried out and analysed in relation to demographic data, comorbidities, treatments, type of vaccine and number of doses.</div></div><div><h3>Results</h3><div>Fifty patients were included. The overall serological response rate was 76%. The cellular response was positive in 54% of patients. The main factors influencing the humoral and cellular responses were age, comorbidities and treatment with anti-CD20.</div></div><div><h3>Conclusion</h3><div>In ID patients, vaccination against COVID-19 can generate an adequate T-cell response, the character of which is an emerging issue in the context of COVID-19 infection. The main limitations of this study and those in the literature are the heterogeneity of the patients included and the absence of a control population. These results highlight the importance of evaluating the antiviral T-cell response and the impact of immunosuppressive treatments.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 3","pages":"Article 116825"},"PeriodicalIF":2.1,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143816260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hand, foot and mouth disease with encephalomyelitis in adult: A case report

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-04-03 DOI: 10.1016/j.diagmicrobio.2025.116832

Kieu Nguyet Oanh Pham , Minh Cuong Duong , Dinh Nam Vo , Dang Trung Nghia Ho

引用次数: 0

CLIA immunoassay as an alternative and accurate method to detect Sars-Cov-2 antigen compared to ELISA

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-04-03 DOI: 10.1016/j.diagmicrobio.2025.116828

Keilla Gomes Machado, Vitória Bertelli, Rafaele Frassini, André Felipe Streck, Mariana Roesch Ely

{"title":"CLIA immunoassay as an alternative and accurate method to detect Sars-Cov-2 antigen compared to ELISA","authors":"Keilla Gomes Machado, Vitória Bertelli, Rafaele Frassini, André Felipe Streck, Mariana Roesch Ely","doi":"10.1016/j.diagmicrobio.2025.116828","DOIUrl":"10.1016/j.diagmicrobio.2025.116828","url":null,"abstract":"<div><h3>Background</h3><div>COVID-19 has caused moderately severe infections in humans over the past few years, leading to >759 million confirmed cases. This situation highlights an urgent need to develop accurate diagnostic tests to monitor infectious disease and to adopt alternative methods such as CLIA to achieve low detection levels of proteins on diagnostic platforms.</div></div><div><h3>Objectives</h3><div>Develop in-house immunoassay for ELISA and CLIA to diagnose COVID-19.</div></div><div><h3>Methods</h3><div>200 nasopharyngeal samples were collected using swabs, placed in tubes with 3 mL of PBS. 1 mL from each sample was used to perform qRT-PCR and was considered positive in samples with CT < 38. The remaining volume was used for in-house sandwich immunoassay ELISA and CLIA.</div></div><div><h3>Results</h3><div>The results showed that CLIA was able to detect active disease in samples containing N protein concentrations greater than 16 ng/mL, with a sensitivity of 90 % and specificity of 94.5 %, and an area under the ROC curve (AUROC) of 0.943 (95 % CI: 0.909–0.977). ELISA showed an AUROC = 0.709 (95 % CI: 0.639–0.778), with a sensitivity of 54.4 % and specificity of 87.2 %.</div></div><div><h3>Conclusions</h3><div>The CLIA results in this study outperformed the traditional ELISA and proved to be a suitable platform for monitoring the progression of disease stages, including the diagnosis of active COVID-19 infection.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 4","pages":"Article 116828"},"PeriodicalIF":2.1,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143817064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of an anti-HCV chemiluminescence assay: Enhancing diagnostic accuracy and reducing false positives in hepatitis C screening

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-04-03 DOI: 10.1016/j.diagmicrobio.2025.116829

Qingyuan Zheng , Yong He , Quanjing Zhu , Chi Wang , Xin Nie

{"title":"Evaluation of an anti-HCV chemiluminescence assay: Enhancing diagnostic accuracy and reducing false positives in hepatitis C screening","authors":"Qingyuan Zheng , Yong He , Quanjing Zhu , Chi Wang , Xin Nie","doi":"10.1016/j.diagmicrobio.2025.116829","DOIUrl":"10.1016/j.diagmicrobio.2025.116829","url":null,"abstract":"<div><h3>Objectives</h3><div>Detection of antibodies is recommended for the diagnosis and effective treatment of hepatitis C. This study evaluated the performance of a new chemiluminescence assay for detecting hepatitis C virus (HCV) antibodies and compared it with previous assays using a comprehensive set of routine and borderline samples.</div></div><div><h3>Methods</h3><div>A total of 2,216 serum samples were included in this study, comprising of 2,121 routine clinical samples and 95 borderline cases (COI range: 0.9-5.0). We compared the Anti-HCV-2 assay's (Mindray Diagnostics, Shenzhen, China) performance with the Anti-HCV-1 assay, assessing key parameters including sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV). Precision was tested using replicate samples, and accuracy was validated through confirmatory RIBA (Mikrogen GmbH, Neuried, Germany) and Elecsys Anti-HCV assays (Roche Diagnostics, Mannheim, Germany). Interference testing was conducted to assess the assay's robustness against common substances found in clinical samples.</div></div><div><h3>Results</h3><div>The Anti-HCV-2 assay demonstrated high sensitivity (98.2 %), specificity (99.1 %), and accuracy (98.7 %) in routine clinical samples. For borderline cases, the accuracy of Anti-HCV-2 was significantly higher (96.84 %) compared to Anti-HCV-1 (3.16 %). Precision testing showed a coefficient of variation (CV) of <2 %, indicating excellent reproducibility. Anti-interference testing confirmed that the Anti-HCV-2 assay performed consistently across samples with common interferences, such as hemolysis and lipemia.</div></div><div><h3>Conclusion</h3><div>The Anti-HCV-2 assay outperforms the Anti-HCV-1 assay in terms of accuracy, reproducibility, and reliability, especially in the range of borderline, making it a valuable tool for routine HCV screening.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 3","pages":"Article 116829"},"PeriodicalIF":2.1,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143760944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Utility of sexually transmitted infection screening in diagnosing clinical gynaecological conditions using endometrial specimens

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-04-02 DOI: 10.1016/j.diagmicrobio.2025.116830

Alfredo Maldonado-Barrueco , Esther Almazán-Garate , Onica Armijo-Suárez , Silvia Iniesta-Pérez , Claudia Sanz-González , Iker Falces-Romero , Covadonga Álvarez-López , Juana Cacho-Calvo , Inmaculada Quiles-Melero

引用次数: 0

Understanding the factors impacting dengue rapid diagnosis in Bangladesh

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-03-29 DOI: 10.1016/j.diagmicrobio.2025.116827

Selim Reza Tony , Mst Noorjahan Begum , Nusrat Sultana , Yeasir Karim , Shaheen Alam , Afsana Rashed , Kamrun Nahar , Ashish Kumar Ghosh , Amirul Huda Bhuiyan , Sultana Shahana Banu , Mustafizur Rahman

{"title":"Understanding the factors impacting dengue rapid diagnosis in Bangladesh","authors":"Selim Reza Tony , Mst Noorjahan Begum , Nusrat Sultana , Yeasir Karim , Shaheen Alam , Afsana Rashed , Kamrun Nahar , Ashish Kumar Ghosh , Amirul Huda Bhuiyan , Sultana Shahana Banu , Mustafizur Rahman","doi":"10.1016/j.diagmicrobio.2025.116827","DOIUrl":"10.1016/j.diagmicrobio.2025.116827","url":null,"abstract":"<div><h3>Background</h3><div>Dengue affects over 70 % of the populations in tropical and subtropical regions, including Bangladesh, posing a significant health burden. The sensitivity of widely used rapid diagnostic tests (RDTs) varies due to factors like viral load, serotypes, secondary infections, and disease severity.</div></div><div><h3>Aims</h3><div>This study evaluated the diagnostic performance of RDTs compared to RT-PCR, identifying key factors affecting sensitivity.</div></div><div><h3>Methods</h3><div>The study at Dhaka Medical College (DMC) involved 683 suspected dengue cases. Blood samples and metadata were collected, and RDTs (NS1, IgG, IgM) were performed on-site. Samples were analyzed at icddr,b using RT-PCR for viral RNA detection and serotyping. Diagnostic performance was assessed using MedCalc, while metadata and sensitivity differences were analyzed with SPSS.</div></div><div><h3>Results</h3><div>We found that vomiting was the most common symptom, followed by, diarrhea, restlessness, and abdominal tenderness. RDT sensitivity was highest in cases with high viral loads, reaching 94 % within 1–3 days of symptom onset and 82 % beyond three days. Moderate viral loads showed sensitivities of 70 % for NS1+IgM and 84 % for NS1+IgM+IgG within three days, decreasing thereafter. Sensitivity was low in low viral load cases but improved after three days with IgG incorporation. DENV-3 and secondary infections showed higher false-negative rates.</div></div><div><h3>Conclusion</h3><div>The study emphasizes the importance of early testing in suspected dengue cases with symptoms like fever, vomiting, diarrhea, or abdominal tenderness, offering critical insights to enhance diagnostics, patient care, and disease control.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 3","pages":"Article 116827"},"PeriodicalIF":2.1,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143760943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0