Diagnostic microbiology and infectious disease最新文献_第3页

Oral fluid versus blood in HIV self-testing: A step towards “95-95-95” targets of the UNAIDS? 艾滋病毒自检中的口腔液与血液：向联合国艾滋病规划署“95-95-95”目标迈进了一步？

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-04-23 DOI: 10.1016/j.diagmicrobio.2025.116867

Pachamuthu Balakrishnan , Ramachandran Vignesh , Pitchaipillai Sankar Ganesh , Saravanan Shanmugam , Iyanar Kannan , Sathasivam Sivamalar , Sivadoss Raju , Vijayakumar Velu , Esaki M Shankar , Sree T. Sucharitha

{"title":"Oral fluid versus blood in HIV self-testing: A step towards “95-95-95” targets of the UNAIDS?","authors":"Pachamuthu Balakrishnan , Ramachandran Vignesh , Pitchaipillai Sankar Ganesh , Saravanan Shanmugam , Iyanar Kannan , Sathasivam Sivamalar , Sivadoss Raju , Vijayakumar Velu , Esaki M Shankar , Sree T. Sucharitha","doi":"10.1016/j.diagmicrobio.2025.116867","DOIUrl":"10.1016/j.diagmicrobio.2025.116867","url":null,"abstract":"<div><div>The population of HIV-infected individuals who are not aware of their HIV status or who do not usually have access to HIV services should be reached by the newer strategies. The WHO's guideline on HIV self-testing (HIV-ST) was based on a boost in the number of tests administered in randomized clinical trials, including those carried out among the general population in regions with a high HIV burden. Therefore, HIV-ST offers tremendous potential as a strategy to enhance testing frequency and improve access to HIV testing among key high-risk populations and their partners. Recent studies have documented that HIV-ST with oral fluid is perceived as a more convenient and also preferred option than using the finger prick blood-based testing due to “fear of prick”, and have also reported of user’s error with finger prick blood-based tests increasing than oral fluid-based test. The major benefit of oral fluid-based HIV-ST is that it makes it easier for first-time users to access testing and is more likely to increase testing frequency among key high-risk populations. The current review summarizes the pros and cons of the HIV-ST devices.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"113 1","pages":"Article 116867"},"PeriodicalIF":2.1,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143912110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The value of targeted next-generation sequencing in the diagnosis and differential diagnosis of paucibacillary pulmonary tuberculosis 靶向新一代测序在少杆菌性肺结核诊断和鉴别诊断中的价值

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-04-22 DOI: 10.1016/j.diagmicrobio.2025.116866

Hu Qianfang, Liu Yan, Chen Yuhan, Zhong Houyu, Wan Tao

{"title":"The value of targeted next-generation sequencing in the diagnosis and differential diagnosis of paucibacillary pulmonary tuberculosis","authors":"Hu Qianfang, Liu Yan, Chen Yuhan, Zhong Houyu, Wan Tao","doi":"10.1016/j.diagmicrobio.2025.116866","DOIUrl":"10.1016/j.diagmicrobio.2025.116866","url":null,"abstract":"<div><h3>Background</h3><div>The diagnostic utility of broad-spectrum pathogen-specific targeted next-generation sequencing (tNGS) for paucibacillary pulmonary tuberculosis (PTB) in general hospital settings remains insufficiently characterized compared to validated Mycobacterium-specific tNGS.</div></div><div><h3>Objective</h3><div>To investigate the clinical value of broad-spectrum pathogen-specific tNGS in diagnosing and differentially diagnosing paucibacillary PTB in real-world clinical practice.</div></div><div><h3>Methods</h3><div>This retrospective study analyzed data from 423 patients admitted to the First Affiliated Hospital of Chongqing Medical University from January 1, 2024, to October 31, 2024. Patients underwent bronchoalveolar lavage fluid (BALF) testing, including acid-fast staining, culture, Xpert MTB/RIF, and tNGS. 47 participants were excluded based on predefined criteria.</div></div><div><h3>Results</h3><div>Among 83 microbiologically confirmed PTB cases, tNGS exhibited significantly higher sensitivity compared to Xpert and culture (91.6 % vs. 79.5 % for Xpert and 73.5 % for culture; <em>p</em><0.001), albeit with marginally lower specificity than Xpert (95.6 % vs. 98.6 %; <em>p</em>=0.01). For 293 non-TB patients, tNGS detected pathogens in 86.7 % (254/293) of BAL samples, identifying 300 pathogens with 47.3 % (142/300) concordance to clinical diagnoses.</div></div><div><h3>Conclusion</h3><div>Broad-spectrum pathogen-specific tNGS demonstrated enhanced sensitivity for diagnosing paucibacillary PTB but slightly reduced specificity relative to Xpert. While it achieved high pathogen detection rates in non-TB pulmonary diseases, its concordance with clinical diagnoses was suboptimal.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"113 1","pages":"Article 116866"},"PeriodicalIF":2.1,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143887227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Performance of a molecular beacon-based qPCR method for detection of fluoroquinolone resistance in Mycobacterium tuberculosis: A study confirmed by sequencing 基于分子信标的qPCR检测结核分枝杆菌氟喹诺酮类药物耐药性的性能：经测序证实的研究

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-04-22 DOI: 10.1016/j.diagmicrobio.2025.116865

Oguz Arı , Rıza Durmaz , Ahmet Arslanturk

{"title":"Performance of a molecular beacon-based qPCR method for detection of fluoroquinolone resistance in Mycobacterium tuberculosis: A study confirmed by sequencing","authors":"Oguz Arı , Rıza Durmaz , Ahmet Arslanturk","doi":"10.1016/j.diagmicrobio.2025.116865","DOIUrl":"10.1016/j.diagmicrobio.2025.116865","url":null,"abstract":"<div><div>Multidrug-resistant tuberculosis (MDR-TB) poses a serious threat to the control and treatment of tuberculosis (TB). Rapid diagnosis of resistant strains via utilization of molecular techniques is of critical importance on the proper management of the patients. The objective of this study was to develop a quantitative polymerase chain reaction (qPCR) method for the detection of mutation-based fluoroquinolone (FQ) resistance in Mycobacterium tuberculosis isolates. Primers and a molecular-beacon probe were designed to target quinolone-resistance-determining-region (QRDR) of the <em>gyr</em>A gene of <em>M. tuberculosis</em>. Amplification conditions and concentrations of primers/probe were optimized, and the effectiveness of the optimized qPCR method was tested on 50 MDR <em>M. tuberculosis</em> strains. To confirm the qPCR results, all strains were also screened for mutations in the <em>gyr</em>A gene using Sanger sequencing. The optimized qPCR method had analytical sensitivity of 96.5 cfu/ml. The method detected FQ resistance in 7 (14 %) of the 50 MDR-strains via either no or significantly decreased fluorescence signal due to mutations associated with FQ resistance. The sequencing of these seven strains detected three resistance-associated mutations (A90V, D94A and D94G). Of the 43 FQ-susceptible strains, 10 strains with wild-type gene sequences yielded strong fluorescent signals above 450 RFU, while the remaining 33 strains harboring a non-resistance-associated mutation (S95T) showed decreased fluorescence signals <350 RFU. The sensitivity and specificity of the qPCR method for detection of the mutations related to resistance was ≥99.9 % and 98 %, respectively. Consequently, the utility of the optimized qPCR method for the identification of mutation-based FQ resistance is promising.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 4","pages":"Article 116865"},"PeriodicalIF":2.1,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143876946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A bibliometric and visual analysis of the research status and hotspots of Pulmonary Aspergillosis based on web of science 基于web of science的肺曲霉病研究现状与热点的文献计量与可视化分析

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-04-20 DOI: 10.1016/j.diagmicrobio.2025.116864

Jianye Li , Qingjun Zhu , Huixin Yan , Ting Ma , Yun An

{"title":"A bibliometric and visual analysis of the research status and hotspots of Pulmonary Aspergillosis based on web of science","authors":"Jianye Li , Qingjun Zhu , Huixin Yan , Ting Ma , Yun An","doi":"10.1016/j.diagmicrobio.2025.116864","DOIUrl":"10.1016/j.diagmicrobio.2025.116864","url":null,"abstract":"<div><h3>Background</h3><div>Pulmonary Aspergillosis (PA) is a common fungal lung infection. Despite recent advancements, bibliometric studies on PA are scarce. This study uses bibliometric methods to analyze current research trends and key topics, offering insights into future directions in the field.</div></div><div><h3>Methods</h3><div>PA-related literature was retrieved from the Web of Science Core Collection (WOSCC) database, and detailed analysis was conducted using CiteSpace, VOSviewer, and Excel 2019 software. This analysis aimed to identify trends and hot topics in the field of PA.</div></div><div><h3>Results</h3><div>A total of 1,715 articles were analyzed from 1900 to 2024. The number of publications has shown steady growth, with a gradual increase from 1990 to 2019, followed by a sharp rise after 2019. The United States leads in this field. The main research hotspots and frontiers in PA include: the close association between PA and immunocompromised conditions, COVID-19 as a new risk factor for PA, and current clinical research focusing on antifungal treatments and enhancing host immunity.</div></div><div><h3>Conclusion</h3><div>This study reveals trends in PA research, notably the sharp increase in publications post-2019. Novel findings include the identification of COVID-19 as a new risk factor for PA and the growing emphasis on antifungal treatments and host immunity enhancement. These insights provide a clearer direction for future clinical and research priorities in PA.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 4","pages":"Article 116864"},"PeriodicalIF":2.1,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143860345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in early respiratory tract microecology associated with neonatal respiratory distress syndrome 新生儿呼吸窘迫综合征与早期呼吸道微生态变化相关

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-04-17 DOI: 10.1016/j.diagmicrobio.2025.116848

Kun Du, Qian Wei, Jin Gao, Xing-Yu Xiong, Jia-Xin Li, Yuan Yuan

{"title":"Changes in early respiratory tract microecology associated with neonatal respiratory distress syndrome","authors":"Kun Du, Qian Wei, Jin Gao, Xing-Yu Xiong, Jia-Xin Li, Yuan Yuan","doi":"10.1016/j.diagmicrobio.2025.116848","DOIUrl":"10.1016/j.diagmicrobio.2025.116848","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to explore the distribution patterns of the early respiratory tract microbiome in infants with neonatal respiratory distress syndrome.</div></div><div><h3>Methods</h3><div>The study involved infants with neonatal respiratory distress syndrome who were admitted to the neonatal department of Kunming Children’s Hospital between July and October 2022. Samples were obtained at two time points: 1 to 3 days post-admission (initial group) and 7 to 10 days post-admission (later group). Pharyngeal swabs and lower respiratory tract sputum samples were analyzed using 16S ribosomal RNA gene sequencing to assess the composition and variations of the early airway microbiome.</div></div><div><h3>Results</h3><div>The predominant microbiota identified in both pharyngeal and lower respiratory tract sputum samples at 1 to 3 days and 7 to 10 days after birth included <em>Streptococcus, Enterobacter, Halomonas, Acinetobacter</em>, and <em>Serratia</em>, with the first three being the most dominant. The microbial distribution patterns were largely consistent between the initial and later groups in both sample types, exhibiting minimal age-related variation. Although the later group demonstrated higher microbial community richness and lower diversity compared to the initial group, these differences were not statistically significant. Notably, significant differences were observed in the richness index, ACE index, and Chao1 index of the pharyngeal microbiota between the initial and later groups.</div></div><div><h3>Conclusion</h3><div>The respiratory tract microbial composition in infants with neonatal respiratory distress syndrome remained relatively stable between 1 and 3 days and 7 to 10 days post-birth, although changes in the abundance and diversity of the respiratory tract microbiota were observed with advancing age.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 4","pages":"Article 116848"},"PeriodicalIF":2.1,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143882199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Performance evaluation of the access anti-HBc IgM Assay on the DxI 9000 access immunoassay analyzer DxI 9000接入免疫分析分析仪上抗- hbc IgM检测的性能评价

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-04-17 DOI: 10.1016/j.diagmicrobio.2025.116862

Catherine Coignard , Claire Vincent , Veronique Lemée , Jean-Christophe Plantier , Jérémie Gautier , Françoise Leboulaire , Marc Turini , Gaiane Demirdjian , Magali Karagueuzian , Vanessa Roulet , Juliane Hey , Dan W. Rhodes

{"title":"Performance evaluation of the access anti-HBc IgM Assay on the DxI 9000 access immunoassay analyzer","authors":"Catherine Coignard , Claire Vincent , Veronique Lemée , Jean-Christophe Plantier , Jérémie Gautier , Françoise Leboulaire , Marc Turini , Gaiane Demirdjian , Magali Karagueuzian , Vanessa Roulet , Juliane Hey , Dan W. Rhodes","doi":"10.1016/j.diagmicrobio.2025.116862","DOIUrl":"10.1016/j.diagmicrobio.2025.116862","url":null,"abstract":"<div><div>This study evaluated the diagnostic and analytical performances of the Access anti-HBc IgM assay (Access assay) for use on the DxI 9000 Access Immunoassay Analyzer. Prospectively and retrospectively collected samples were tested with Access and a comparator assay with use of a second comparator for discrepant resolution to determine final anti-HBc IgM sample status. Specificity of Access was 100.00 % (99.65 – 100.00 %) on 1,098 anti-HBc IgM negative blood donor samples, 100.00 % (98.74 – 100.00 %) on 300 anti-HBc IgM negative hospitalized patient samples and 94.08 % (89.45 – 96.75 %) on 169 anti-HBc IgM negative acute/recent and chronic HBV infected patient samples. Sensitivity was 100.00 % (98.42 – 100.00 %) on 239 anti-HBc IgM positive acute/recent and chronic HBV infected patient samples. Seroconversion panels showed mean first day of detection 3.2 days earlier with Access than with the comparator assay. Maximum reproducibility on positive samples was 8.6 % coefficient of variance (CV) and 0.048 S/CO standard deviation (SD) on negative samples.</div><div>The Access anti-HBc IgM assay demonstrated excellent diagnostic and analytical performances comparable to other current CE-marked anti-HBc IgM assays.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 4","pages":"Article 116862"},"PeriodicalIF":2.1,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143882200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel highly sensitive and specific multiplex real-time pcr assay for detection of Leishmania donovani and human immunodeficiency virus-1 co-infection 一种检测多诺瓦利什曼原虫和人类免疫缺陷病毒-1合并感染的高灵敏度和特异性多重实时pcr新方法

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-04-16 DOI: 10.1016/j.diagmicrobio.2025.116860

Moses Sanya , Victor Atunga Mobegi , Bernard Ongóndo Osero , Damaris Matoke-Muhia , Clement Shiluli , Juliette Rose Ongus

{"title":"Novel highly sensitive and specific multiplex real-time pcr assay for detection of Leishmania donovani and human immunodeficiency virus-1 co-infection","authors":"Moses Sanya , Victor Atunga Mobegi , Bernard Ongóndo Osero , Damaris Matoke-Muhia , Clement Shiluli , Juliette Rose Ongus","doi":"10.1016/j.diagmicrobio.2025.116860","DOIUrl":"10.1016/j.diagmicrobio.2025.116860","url":null,"abstract":"<div><div>The co-infection of <em>Leishmania donovani</em> the etiological agent of visceral leishmaniasis (VL) and Human Immunodeficiency Virus-1 (HIV-1) poses a significant public health challenge, especially in regions where these pathogens have geographical overlaps. Individuals with this coinfection often have severe morbidity and mortality. Diagnosis of VL-HIV coinfections can be challenging due to atypical clinical presentations and overlapping manifestations with other infections. Serological diagnosis of VL is often poor, due to low humoral response in HIV-infected individuals characterized by reduced antibody production making antibody tests difficult hence the need to develop a more sensitive and specific diagnosis assay for both pathogens. In this study, oligonucleotides were designed to target the long terminal repeat (LTR) and internal transcribed spacer 1 (ITS1) in the HIV-1and <em>L. donovani</em> genomes respectively. The human beta-actin gene was used as the internal control. The analytical sensitivity and specificity were tested using ATCC DNA standards and <em>L. major</em> culture DNA. Diagnostic performance was tested using conventional PCR characterized clinical samples. The limit of detection of the multiplex assay was 1 fg/μL and 100 fg/μL for HIV-1 and <em>L. donovani</em> targets respectively. No cross-reactivity was observed, and the assay diagnostic performance was comparable to the conventional PCR (kappa = 1). With the advantages of high efficiency and rapid and accurate diagnosis of multiplex real-time RT-PCR, the data obtained shows that this assay is very feasible for the diagnosis of VL-HIV-1 co-infection and would be crucial in proper treatment, and monitoring and would lead to reduced treatment failure and recurrent VL episodes.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 4","pages":"Article 116860"},"PeriodicalIF":2.1,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143873900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of a cost-effective gating strategy for CD4+ T lymphocyte enumeration in HIV-infected individuals via flow cytometry 通过流式细胞术评估hiv感染者CD4+ T淋巴细胞计数的成本效益控制策略

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-04-16 DOI: 10.1016/j.diagmicrobio.2025.116851

Selim Merdan , Yağmur Ekenoğlu Merdan , Okan Aydoğan

{"title":"Evaluation of a cost-effective gating strategy for CD4+ T lymphocyte enumeration in HIV-infected individuals via flow cytometry","authors":"Selim Merdan , Yağmur Ekenoğlu Merdan , Okan Aydoğan","doi":"10.1016/j.diagmicrobio.2025.116851","DOIUrl":"10.1016/j.diagmicrobio.2025.116851","url":null,"abstract":"<div><h3>Background</h3><div>Monitoring CD4+ <em>T</em> lymphocyte levels is a cornerstone in managing HIV infection, essential for assessing immune status and determining treatment strategies. However, the high cost of standard flow cytometry methods limits access to these diagnostic tools in low-income settings. This study evaluates a cost-effective alternative method (AM) with a simplified gating strategy and reduced antibody use compared to the standard method (SM), which is based on PanLeucogating (PLG).</div></div><div><h3>Methods</h3><div>This multicenter study analyzed 1,001 HIV-positive patient samples in a central laboratory serving six hospitals in Istanbul. SM used four antibodies (CD45/CD3/CD4/CD8), while AM utilized only CD4/CD8. Flow cytometric analysis was performed on a BD FACS Canto II system, and CD4⁺ T cell percentages were compared between methods using Pearson’s correlation coefficient and the Mann-Whitney U test.</div></div><div><h3>Results</h3><div>A strong correlation (<em>r</em> = 0.993, <em>p</em> < 0.001) was observed between AM and SM, with no statistically significant difference (<em>p</em> = 0.339). However, AM systematically reported lower CD4⁺ percentages, likely due to the absence of CD3-based gating and the use of FS/SS plots instead of CD45 for gating lymphocytes.</div></div><div><h3>Conclusion</h3><div>With one of the largest datasets among similar studies, our findings suggest that AM, which uses 50 % fewer antibodies, is a cost-effective and viable alternative for CD4⁺ enumeration in HIV monitoring. However, further validation in diverse epidemiological settings and clinical impact assessments are required before widespread clinical implementation.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 4","pages":"Article 116851"},"PeriodicalIF":2.1,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143850376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effective treatment of listeria liver abscesses with high-dose oral antibiotics: Case insights and review of clinical challenges 大剂量口服抗生素有效治疗李斯特菌肝脓肿：病例分析和临床挑战回顾

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-04-16 DOI: 10.1016/j.diagmicrobio.2025.116861

Rabindra Dhakal , Gabriel Chavez Reyna , Bidur Mainali , Ujwal Basnet , Allison Glaser

{"title":"Effective treatment of listeria liver abscesses with high-dose oral antibiotics: Case insights and review of clinical challenges","authors":"Rabindra Dhakal , Gabriel Chavez Reyna , Bidur Mainali , Ujwal Basnet , Allison Glaser","doi":"10.1016/j.diagmicrobio.2025.116861","DOIUrl":"10.1016/j.diagmicrobio.2025.116861","url":null,"abstract":"<div><div><em>Listeria monocytogenes</em> (<em>L. monocytogenes)</em> is a rare but serious pathogen, particularly in immunocompromised individuals. Hepatic listeriosis is exceptionally uncommon, making this case of multiple liver abscesses in a 55-year-old woman notable. The patient, with type 2 diabetes mellitus (T2DM), non-alcoholic fatty liver disease (NAFLD), and euglycemic diabetic ketoacidosis (eDKA) likely triggered by an SGLT2 inhibitor, presented with hypodense hepatic lesions initially suspected as metastatic disease. Mild liver enzyme elevations complicated the diagnosis, which was confirmed via blood cultures and histopathology. She responded well to a six-week course of oral amoxicillin and metronidazole, avoiding surgical intervention. This case emphasizes the need to consider infectious etiologies in immunocompromised patients with atypical imaging findings. It also highlights the role of eDKA in increasing infection susceptibility and demonstrates that conservative, non-invasive management can be effective in select cases, challenging conventional treatment approaches.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 4","pages":"Article 116861"},"PeriodicalIF":2.1,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143876947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibiotic resistance and bacterial spectrum in cerebrospial fluid cultures: Initial insights from Pakistan 脑脊液培养中的抗生素耐药性和细菌谱：来自巴基斯坦的初步见解

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-04-15 DOI: 10.1016/j.diagmicrobio.2025.116847

Zafar Mehmood , Noman Asghar , Ihtisham Ullah , Ali Talha Khalil , Arshad Islam , Majid Khan , Sami Ullah , Siyab Ali , Sadiq Ali Shah

{"title":"Antibiotic resistance and bacterial spectrum in cerebrospial fluid cultures: Initial insights from Pakistan","authors":"Zafar Mehmood , Noman Asghar , Ihtisham Ullah , Ali Talha Khalil , Arshad Islam , Majid Khan , Sami Ullah , Siyab Ali , Sadiq Ali Shah","doi":"10.1016/j.diagmicrobio.2025.116847","DOIUrl":"10.1016/j.diagmicrobio.2025.116847","url":null,"abstract":"<div><div>Antimicrobial resistance (AMR) is a rising global health concern, especially in resource-limited countries like Pakistan, where indiscriminate and unregulated use of antibiotics has significantly worsened this problem. The study aimed to analyze the bacterial spectrum and antimicrobial resistance patterns in cerebrospinal fluid (CSF) cultures from patients with suspected bacterial meningitis, providing crucial insights for better infection control and treatment strategies.This retrospective study analyzed 1107CSF cultures were between 2019 and 2024 at the Lady Reading Hospital, MTI, Khyber Pakhtunkhwa, Pakistan, to assess bacterial profiles in patients with suspected bacterial meningitis and investigate the AMR patterns. Among the 106 positive growth cultures, gram-negative bacteria accounted for 84.9 %. Alarmingly, <em>Acinetobacter baumannii</em> was found to be completely resistant to all the nine antibiotics tested, while <em>Salmonella typhi</em> showed resistance to six out of seven tested antibiotics, highlighting their severe multi-drug resistance profile. Moreover, notable resistance was observed in <em>Citrobacter freundii, Enterobacter</em> species, and <em>Staphylococcus aureus</em>. The distribution results indicated that the <em>Klebsiella pneumoniae</em> is more prevalent in males and the 1–13 years age group. These findings highlight the necessity for urgent action on antibiotic stewardship, improved surveillance, and public awareness to mitigate the spread of AMR pathogens, particularly in life-threatening infections such as bacterial meningitis. Strengthening infection control measures and regulating antibiotic usage policies is imperative to curb the growing AMR crisis.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 4","pages":"Article 116847"},"PeriodicalIF":2.1,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143833361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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