在唾液中进行无创分子登革病毒1型诊断是可能的

IF 2.1 4区医学 Q3 INFECTIOUS DISEASES

Diagnostic microbiology and infectious disease Pub Date : 2025-05-31 DOI:10.1016/j.diagmicrobio.2025.116935

Carolina Gracia Poitevin , Carla Adriane Royer , Ana Claudia Bonatto , Cristina de Oliveira Rodrigues , Gabrielle Caroline Peiter , Ana Paula Carneiro Brandalize , Alexia Cristine Oliveira Rocha , Carolina Langaro Brockmann , Gabriela Bravim de Araujo , Giovanna Lourenço Cavagnoli , Ingrid Vitória Simardo , Valentina Miotto Gasparim , Regiane Stafim da Cunha , Vanessa Crocetti Pereira , Edneia Amancio de Souza Ramos , Pedro Henrique Cardoso , Monica Barcellos Arruda , Patrícia Alvarez da Silva Baptista , Emanuel Maltempi de Souza , Daniela Fiori Gradia , Jaqueline Carvalho de Oliveira

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引用次数: 0

摘要

目的登革热是一种全身性病毒感染，在热带/亚热带地区流行，在全球日益重要。急性感染诊断的金标准是通过血液中逆转录定量聚合酶链反应（RT-qPCR）检测病毒RNA。遗憾的是，静脉采集需要专业的专业人员，而那些害怕针头的人可能会避免登革热检测。因此，本研究的目的是评估利用唾液检测DENV-1的可行性。方法采用srt - qpcr检测201对唾液和血浆样本中的DENV-1，这些样本来自症状出现5天内的个体。结果血浆DENV-1阳性140例（69.65%），唾液DENV-1阳性115例（57.21%）。这些值的敏感性为82.14%，特异性为100%（一致性kappa指数0.736[0.69 - 0.77]）。尽管敏感性降低，但我们证明了在唾液样本中诊断DENV-1的可行性，提出了一种有价值的替代方法，特别是在疫情暴发的情况下。

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Non-Invasive molecular dengue virus 1 diagnosis in saliva is possible

Objectives

Dengue is a systemic virus infection, endemic in tropical/subtropical regions and of increasing global importance. The diagnostic gold standard for acute infection is the detection of viral RNA through reverse transcription quantitative polymerase chain reaction (RT-qPCR) in blood. Regrettably, venous collection requires specialized professionals, and dengue tests may be avoided by those who afraid of needles. Therefore, the aim of the present study is to evaluate the feasibility of using saliva in DENV-1 detection.

Methods

RT-qPCR was performed to detect DENV-1 in 201 paired saliva and plasma samples, from individuals with up to five days from the onset of symptoms.

Results

140 (69.65 %) patients had DENV-1 positive detection in plasma and 115 (57.21 %) positive in saliva samples. Those values rendered a sensitivity of 82.14 % and 100 % of specificity (agreement kappa index 0.736 [0.69 - 0.77].

Conclusions

Despite reduced sensitivity, we demonstrated the feasibility of DENV-1 diagnosis in saliva samples, presenting a valuable alternative, particularly in outbreak scenario.

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来源期刊

Diagnostic microbiology and infectious disease 医学-传染病学

CiteScore

5.30

自引率

3.40%

发文量

149

审稿时长

56 days

期刊介绍： Diagnostic Microbiology and Infectious Disease keeps you informed of the latest developments in clinical microbiology and the diagnosis and treatment of infectious diseases. Packed with rigorously peer-reviewed articles and studies in bacteriology, immunology, immunoserology, infectious diseases, mycology, parasitology, and virology, the journal examines new procedures, unusual cases, controversial issues, and important new literature. Diagnostic Microbiology and Infectious Disease distinguished independent editorial board, consisting of experts from many medical specialties, ensures you extensive and authoritative coverage.