Autobio BC60血培养系统中阳性血培养物EUCAST快速药敏试验的评价

IF 2.1 4区医学 Q3 INFECTIOUS DISEASES

Diagnostic microbiology and infectious disease Pub Date : 2025-06-04 DOI:10.1016/j.diagmicrobio.2025.116937

Serap Süzük Yıldız , Esra Tavukcu , Mert Emre Ölmez , Sevgi Şahin , Habibe Kurtaran Tek , Can Hüseyin Hekimoğlu , Ayşe Semra Güreser , İpek Mumcuoğlu , Tuba Dal

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引用次数: 0

摘要

早期诊断和有效治疗可显著改善血流感染的预后。欧洲药敏试验委员会（EUCAST）开发了基于纸片扩散法的快速药敏试验（RAST）。在本研究中，我们比较了Autobio BC （Autobio diagnostics，中国郑州）血液培养系统中阳性血培养的RAST研究数据与使用标准圆盘扩散法获得的数据。共收集234份加标血培养标本（肺炎克雷伯菌[n= 50]、大肠杆菌[n= 50]、鲍曼不动杆菌[n= 20]、铜绿假单胞菌[n= 13]、金黄色葡萄球菌[n= 51]、肠球菌[n= 50]）。阳性瓶进行RAST试验，分别于4、8、20 h测定结果。进行标准圆盘扩散（Standard disk diffusion, sDD），测定血培养瓶传代样品的区直径。将RAST区直径与sDD区直径进行比较。基于第4、8、20 h可读区直径的分类一致性为肺炎克雷伯菌（99.7%、99.7%、99.7%）、大肠杆菌（99.4%、99.6%、99.7%）、鲍曼假杆菌（98.7%、98.7%、98.7%）、铜绿假单胞菌（100%、99.3%、100%）、金黄色葡萄球菌（100%、100%、100%）和肠球菌（100%、100%、100%）。整个研究得到的小误差（mE）、大误差（mE）和甚大误差（VME）结果分别为0.9、0和1.3，持续4 h；1.4、0、1.3为8小时；EUCAST RAST研究可以在Autobio BC血液培养系统的阳性瓶中进行，结果可靠。为了报告早期的抗生素敏感性试验结果，使用Autobio BC的实验室可以将RAST与质量控制研究结合起来。

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Evaluation of EUCAST rapid antibiotic susceptibility testing for positive blood cultures in the Autobio BC60 blood culture system

Early diagnosis and effective treatment of bloodstream infections significantly improve prognosis. The European Committee on Antimicrobial Susceptibility Testing (EUCAST) has developed rapid antimicrobial susceptibility testing(RAST) based on the disk diffusion method. In the present study, we compared the data from the RAST study from positive blood cultures in the Autobio BC (Autobio-diagnostics, Zhengzhou, Chinese) blood culture system with those obtained using the standard disk diffusion method.

A total of 234 spiked blood culture samples (Klebsiella penumoniae [n= 50], Escherichia coli [n= 50], Acinetobacter baumannii [n= 20], Pseudomonas aeruginosa [n= 13], Staphylococcus aureus [n= 51], and Enterococcus spp. [n= 50]) were collected. The RAST test was performed on positive bottles, and the results were measured at 4, 8, and 20 h. Standard disk diffusion (sDD) was performed, and zone diameters were measured from subculture samples obtained from blood culture bottles. The RAST zone diameters were compared with the sDD zone diameters.

The categorical agreement based on the readable zone diameters for the 4th, 8th, and 20th h was K.pneumoniae (99.7 %,99.7 %,99.7 %), E.coli (99.4 %,99.6 %,99.7 %), A.baumannii (98.7 %,98.7 %,98.7 %), P.aeruginosa (100 %,99.3 %,100 %), S. aureus (100 %,100 %,100 %), and Enterococcus spp. (100 %,100 %,100 %). The minor error(mE), major error(ME), and very major error(VME) results obtained in the whole study were determined as 0.9, 0, and 1.3 for 4 h; 1.4, 0, and 1.3 for 8 h; 0.6, 0, and 1.3 for 20 h.

The EUCAST RAST study can be performed from positive bottles in the Autobio BC blood culture system, resulting in reliable outcomes. To report earlier antibiotic susceptibility test results, laboratories using Autobio BC can combine RAST with quality control studies.

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来源期刊

Diagnostic microbiology and infectious disease 医学-传染病学

CiteScore

5.30

自引率

3.40%

发文量

149

审稿时长

56 days

期刊介绍： Diagnostic Microbiology and Infectious Disease keeps you informed of the latest developments in clinical microbiology and the diagnosis and treatment of infectious diseases. Packed with rigorously peer-reviewed articles and studies in bacteriology, immunology, immunoserology, infectious diseases, mycology, parasitology, and virology, the journal examines new procedures, unusual cases, controversial issues, and important new literature. Diagnostic Microbiology and Infectious Disease distinguished independent editorial board, consisting of experts from many medical specialties, ensures you extensive and authoritative coverage.