Diagnostic microbiology and infectious disease最新文献

{"title":"Sensitively detecting antigen of SARS-CoV-2 by a homogeneous immunoassay based on ortho-touch reaction and graphene oxide (GO) quenched acridine esterification chemiluminescence","authors":"Zhifeng Li ,&nbsp;Juntao Ma ,&nbsp;Jie Hong ,&nbsp;Huiyan Yu ,&nbsp;Yuxin Chen ,&nbsp;Liguo Zhu ,&nbsp;Changjun Bao","doi":"10.1016/j.diagmicrobio.2025.116849","DOIUrl":"10.1016/j.diagmicrobio.2025.116849","url":null,"abstract":"<div><div>In this study, a homogeneous immunoassay based on ortho-touch reaction and graphene oxide (GO) quenched acridine esterification chemiluminescence is developed for sensitive detection of SARS-CoV-2 antigen. The sensitivity and specificity of this method was further evaluated with viral protein, virus strains and clinical samples. Benefiting from strong chemiluminescence signal and good quenching performance of GO, this method allows enhanced signal-to-background ratio, and the limit of detection is down to 0.02 ng/ml of viral protein or equivalently 200 copies/ml of virus strains. In clinical evaluations, our approach demonstrated a high level of agreement with digital RT-PCR, achieving a positive coincidence rate of 90.87 %, a negative coincidence rate of 99.77 %, and an overall concordance of 96.53 %, while showing no cross-reactivity with other prevalent viruses. This method offers a promising, sensitive, rapid, and cost-effective platform for early SARS-CoV-2 diagnosis. Moreover, it can potentially be adapted for detecting other pathogens.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 4","pages":"Article 116849"},"PeriodicalIF":2.1,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143860347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Minimum inhibitory concentration of penicillin as a surrogate for in vitro piperacillin susceptibility of ampicillin-susceptible Enterococcus faecalis","authors":"Kohei Hasegawa ,&nbsp;Kumiko Suzuki ,&nbsp;Kenya Murata ,&nbsp;Yoshihiko Ogawa","doi":"10.1016/j.diagmicrobio.2025.116850","DOIUrl":"10.1016/j.diagmicrobio.2025.116850","url":null,"abstract":"<div><div>This study evaluated whether penicillin MICs can predict piperacillin susceptibility in <em>Enterococcus faecalis</em>. Among 427 strains, 10 were resistant to piperacillin. Even strains with penicillin MICs of 4-8 µg/mL may exhibit resistance. These findings suggest that even penicillin MICs within the susceptible range may indicate piperacillin resistance, challenging CLSI guidelines.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 4","pages":"Article 116850"},"PeriodicalIF":2.1,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143848386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ophthalmic manifestations of Monkeypox: An emerging concern","authors":"Rabab Fatima","doi":"10.1016/j.diagmicrobio.2025.116852","DOIUrl":"10.1016/j.diagmicrobio.2025.116852","url":null,"abstract":"","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 4","pages":"Article 116852"},"PeriodicalIF":2.1,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vitro activity of ceftobiprole against 20,255 recent clinical bacterial isolates in Canada (CANWARD 2015-2023)","authors":"Élodie Gemme ,&nbsp;Andrew Walkty ,&nbsp;Melanie Baxter ,&nbsp;Heather J. Adam ,&nbsp;Philippe Lagacé-Wiens ,&nbsp;Karl Weiss ,&nbsp;James A. Karlowsky ,&nbsp;George G. Zhanel ,&nbsp;the Canadian Antimicrobial Resistance Alliance (CARA)","doi":"10.1016/j.diagmicrobio.2025.116838","DOIUrl":"10.1016/j.diagmicrobio.2025.116838","url":null,"abstract":"<div><h3>Background</h3><div>Ceftobiprole is a fifth-generation cephalosporin active against methicillin-resistant <em>Staphylococcus aureus</em> (MRSA). In this study, we determined the <em>in vitro</em> activities of ceftobiprole and comparator agents against 20,225 common Gram-positive and Gram-negative bacteria isolated from patients who presented for care at 16 tertiary-care hospitals across Canada between 2015 and 2023.</div></div><div><h3>Methods</h3><div>Minimum inhibitory concentrations (MICs) were determined using the Clinical and Laboratory Standards Institute (CLSI) reference M07 broth microdilution method. MICs were interpreted using CLSI M100 breakpoints where available. As CLSI does not publish MIC breakpoints for ceftobiprole, ceftobiprole MICs were interpreted using United States Food and Drug Administration (FDA), European Committee on Antimicrobial Susceptibility Testing (EUCAST), and Health Canada breakpoints.</div></div><div><h3>Results</h3><div>Ceftobiprole inhibited 100 % of MRSA at ≤ 4 μg/ml; 100 % of methicillin-susceptible <em>S. aureus</em> (MSSA) and 99.8% of MRSA at ≤ 2 μg/ml; 100 % of <em>Streptococcus pneumoniae</em> and <em>Streptococcus pyogenes</em> at ≤ 0.5 μg/ml; and 97.9 % of ESBL-negative <em>Escherichia coli</em>, 97.4 % of ESBL-negative <em>Klebsiella pneumoniae</em>, 97.8 % of <em>Proteus mirabilis</em>, 89.3 % of <em>Serratia marcescens</em>, 78.2 % of <em>Enterobacter cloacae</em>, 55.7 % of <em>Klebsiella oxytoca</em> at ≤ 0.25 μg/ml. Ceftobiprole was inactive against ESBL-positive <em>E. coli</em> and ESBL-positive <em>K. pneumoniae</em>.</div></div><div><h3>Conclusions</h3><div>Ceftobiprole demonstrated potent <em>in vitro</em> activity against MRSA, MSSA, <em>S. pneumoniae, S. pyogenes</em>, ESBL-negative <em>E. coli</em> and <em>K. pneumoniae</em>, and <em>P. mirabilis</em> isolated from clinical specimens of patients seeking care at Canadian tertiary-care hospitals.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 4","pages":"Article 116838"},"PeriodicalIF":2.1,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143828917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gaps between the days of antibiotic spectrum coverage (DASC) and clinical sense of antimicrobial spectrum","authors":"Tsukasa Higashionna ,&nbsp;Yohei Manabe ,&nbsp;Hideharu Hagiya","doi":"10.1016/j.diagmicrobio.2025.116839","DOIUrl":"10.1016/j.diagmicrobio.2025.116839","url":null,"abstract":"<div><div>Days of Antibiotic Spectrum Coverage (DASC) is a novel metric assessing antibiotic use in stewardship. This study evaluated its validity by comparing ASC scores with Japanese pharmacists’ evaluations. Discrepancies in ASC scores for some antimicrobial agents indicate that local adjustments are needed to enhance clinical relevance and acceptability.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 4","pages":"Article 116839"},"PeriodicalIF":2.1,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143873965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"“What am I?” microbiology of culture-positive, biofire® blood culture identification 2 panel-negative bloodstream infections","authors":"Joseph Berger ,&nbsp;Divya Pandya ,&nbsp;Jordan Colson ,&nbsp;Octavio V. Martinez ,&nbsp;Anthony D. Anderson ,&nbsp;Jose F. Camargo","doi":"10.1016/j.diagmicrobio.2025.116846","DOIUrl":"10.1016/j.diagmicrobio.2025.116846","url":null,"abstract":"<div><h3>Background</h3><div>The BioFire® blood culture identification 2 panel (BCID2) detects 33 of the most common bloodstream infection (BSI) pathogens, yet it can fail to detect the causal agent of sepsis in up to 13 % of cases, mostly due to infections caused by off-panel target microorganisms. A better understanding of the microbiology of culture-positive, BCID2-negative BSI is needed.</div></div><div><h3>Methods</h3><div>Single-center, retrospective study of microbiology and outcomes in 275 cases of BCID2-negative BSI between 2022 and 2024.</div></div><div><h3>Results</h3><div>A total of 257 adult patients with 275 cases of culture-positive, BCID2-negative BSI were analyzed. Viable organisms were identified in 95.2 % of the cases. False negative results (i.e., in-BCID2 panel target microorganism) occurred at 3.3 % and corresponded mostly to <em>Candida</em> spp. (67 %). Although microorganisms considered of low clinical significance accounted for more than a third of the cases, off-BCID2 panel, clinically relevant microorganisms identified by culture included <em>Bacteroides</em> spp. (other than <em>B. fragilis</em>; 12 %), <em>Clostridium</em> spp. (5.8 %), <em>Candida</em> spp. (4.4 %), <em>Achromobacter xylosoxidans, Pasteurella multocida, Trichosporon asahii, Fusarium</em> spp.<em>, Rhodococcus equi, Capnocytophaga</em> spp.<em>,</em> and <em>Burkholderia cepacia,</em> among others<em>.</em> Only 116 (42 %) were on appropriate antibiotic therapy at the time of BCID2 result. 30-day mortality was 24 % in the entire cohort, but as high as 33 %, 44 %, and 42 % for <em>Bacteroides</em> spp., <em>Clostridium</em> spp. and <em>Candida</em> BSI, respectively.</div></div><div><h3>Conclusions</h3><div>These results highlight the limitations of the BCID2 panel (e.g., need for inclusion of pan-<em>Bacteroides</em> and <em>Clostridium</em> spp.<em>,</em> and optimization of sensitivity for <em>Candida</em> spp.); and provide useful insights on the most common causes of BCID2-negative BSI.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 4","pages":"Article 116846"},"PeriodicalIF":2.1,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143848385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation of Lancefieldella parvula in Secondary Infection from Human Bite: A Rare Occurrence","authors":"Samuel Jiménez Rayas ,&nbsp;Mercedes Isabel Cervantes Hernández ,&nbsp;Édgar Samuel Vanegas Rodríguez ,&nbsp;Claudia Adriana Colin Castro ,&nbsp;María Guadalupe Martínez Zavaleta ,&nbsp;Rafael Franco Cendejas ,&nbsp;Luis Esaú López Jácome","doi":"10.1016/j.diagmicrobio.2025.116842","DOIUrl":"10.1016/j.diagmicrobio.2025.116842","url":null,"abstract":"<div><h3>Background</h3><div>Human bite infections most commonly manifest as skin and soft tissue infections with lower infection rates than animal bites. <em>Lancefieldella parvula</em> is an anaerobe Gram-positive coccus found in human oral mucosa.</div></div><div><h3>Case Presentation</h3><div>We present the case of a 28-year-old healthy woman who suffered a deep skin and soft tissue infection in the left hand after a human bite. She presented with signs and symptoms of infection unresponsive to antibiotics, requiring admission for surgical debridement. Microbiological cultures were taken from the wound and empirical treatment was initiated with ciprofloxacin and clindamycin. Cultures were positive for <em>Lancefieldella parvula</em> by Matrix Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) which was genetically confirmed by 16S sequencing. The patient required two surgical debridements and digit amputation; and was treated with levofloxacin for five days recovered, achieving complete resolution of symptoms.</div></div><div><h3>Conclusions</h3><div>Timely surgical management and targeted antimicrobial therapy in severe human bite infections is crucial. The identification of <em>Lancefieldella parvula</em> using advanced tools like MALDI-TOF emphasizes the need to consider uncommon microorganisms in the differential diagnosis.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 4","pages":"Article 116842"},"PeriodicalIF":2.1,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143833362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An unusual subcutaneous mycosis due to Parathyridaria percutanea in an immunocompromised individual","authors":"Pooja Naharia ,&nbsp;Santosh Karade ,&nbsp;Rajiv Kumar ,&nbsp;Anita Sharma","doi":"10.1016/j.diagmicrobio.2025.116836","DOIUrl":"10.1016/j.diagmicrobio.2025.116836","url":null,"abstract":"<div><div>Solid Organ transplant recipients are at risk of opportunistic infections due to immunosuppressed state, prolonged hospital stay and exposure to multiple antibiotics. Subcutaneous mycoses are heterogenous group of fungal infections affecting dermis and subcutaneous tissue due to penetrating injury. Subcutaneous mycosis in immunocompromised individual causes significant morbidity in the form of chronic discharging sinuses and systemic spread. <em>Parathyridaria percutanea</em> is an emerging cause of subcutaneous mycosis in transplant patients and is difficult to identify phenotypically. It is relatively novel pathogen and only few cases are reported in Indian population. Here we present a case of subcutaneous mycosis who presented with multiple discharging sinuses over left foot. Timely identification of implicating pathogen and initiation of targeted anti-fungal therapy plays important role in decreasing hospital stay and overall financial burden to patient. Present case also highlights importance of genotypic methods in diagnosing emerging fungal infection in immunocompromised individuals, when phenotypic identification fails.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 4","pages":"Article 116836"},"PeriodicalIF":2.1,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143848382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a chimeric 56-21 kDa antigen-based ELISA for serodiagnosis of Orientia tsutsugamushi infection","authors":"Xianxian Zhang ,&nbsp;Zhongqiu Teng ,&nbsp;Ting Mo ,&nbsp;Na Zhao ,&nbsp;Hongyu Ren ,&nbsp;Xinting Li ,&nbsp;Lupeng Dai ,&nbsp;Xue Zhang ,&nbsp;Tian Qin","doi":"10.1016/j.diagmicrobio.2025.116841","DOIUrl":"10.1016/j.diagmicrobio.2025.116841","url":null,"abstract":"<div><div>At present, indirect fluorescent antibody testing (IFA) for the traditional diagnosis of scrub typhus (ST) requires high biosafety facilities to prepare whole bacterial antigens that can cross-react with similar diseases. IFA is also not suitable for large-scale epidemiological investigation. Therefore, a simple, fast, and safe serological diagnostic method with high sensitivity and specificity is needed. In this study, a chimeric protein was expressed that binds to 56-kDa proteins from Karp and Kawasaki serotypes and 21-kDa from Gilliam. The chimeric protein was used to establish an Enzyme-Linked Immunosorbent Assay (ELISA) to detect antibodies against <em>Orientia tsutsugamushi</em> in serum. ELISA had no cross-reaction with spot fever, murine typhus, and Q fever. Compared with IFA, the sensitivity and specificity of ELISA were 94.9 % and 97.2 %, respectively, and it detected the serotypes prevalent in China, including Karp, Gilliam, Kato, and Boryong in the initial application. The results showed that ELISA could be used for serological diagnosis of ST and large-scale serological investigation. Large-scale surveys in endemic areas can be conducted for further evaluation.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 4","pages":"Article 116841"},"PeriodicalIF":2.1,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143860346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A case of lyme lymphocytic meningoradiculitis","authors":"Ken Koon Wong ,&nbsp;Ahmed Itrat ,&nbsp;Kamran Kadkhoda","doi":"10.1016/j.diagmicrobio.2025.116844","DOIUrl":"10.1016/j.diagmicrobio.2025.116844","url":null,"abstract":"<div><div>Lyme disease is the most common tick-borne disease in the United States. Although 15 % of untreated cases develop neurological signs and symptoms but where occurs, it can be life-threatening or at least incapacitating. Here we report a rare case of Lyme lymphocytic meningoradiculitis in a patient with no travel history to Europe. It is important for practitioners in neurology and infectious diseases to be familiar with the protean clinical manifestations of Lyme disease. Our case also harbors important laboratory test utilization note.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"112 4","pages":"Article 116844"},"PeriodicalIF":2.1,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143845016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}