Diagnostic microbiology and infectious disease最新文献

{"title":"Development of a rezafungin isolate verification panel and comparison of reference method to ThermoFisher Sensititre YeastOne panel MIC results","authors":"Jeanna DiFranco-Fisher,&nbsp;Laura Koeth","doi":"10.1016/j.diagmicrobio.2025.117100","DOIUrl":"10.1016/j.diagmicrobio.2025.117100","url":null,"abstract":"<div><div>Rezafungin is indicated for the treatment of adults with limited or no alternative treatment options for candidemia and invasive candidiasis. The development of a rezafungin isolate verification panel is described. A total of 35 <em>Candida</em> species isolates were chosen for initial testing and 30 isolates were selected for additional testing including <em>C. albicans</em> (<em>n</em> = 11), <em>C. glabrata</em> (<em>n</em> = 8), <em>C. tropicalis</em> (<em>n</em> = 7) and <em>C. parapsilosis</em> (<em>n</em> = 4). Modal MIC, range of results and categorical agreement results (based on an acceptable ±2 dilution from the modal MIC) were calculated. Both FDA STIC and CLSI breakpoints were applied to calculated categorical agreement. The replicate reference BMD (rBMD) MIC results spanned an average of 2.4-dilution range and were within ±1 dilution of the modal rBMD for 26/30 (86.7 %) isolates and within ±2 dilutions for all isolates (100 %). The final panel of isolates was used to compare rBMD MIC values with those generated using the Sensititre™ YeastOne™ panel. The correlation of YeastOne results to modal rBMD results was particularly good; 26 out of 30 isolates (86.7 %) were within ±1 dilution of the modal rBMD and 100 % were within ±2 dilutions. When categorical agreement was calculated using CLSI and FDA breakpoints, CLSI criteria performed better than FDA, albeit this was associated with a limited number of isolates with MIC results near the breakpoints. The availability of a curated verification panel with rezafungin modal MIC values will be a valuable resource for laboratories as they incorporate testing devices containing rezafungin into their yeast susceptibility testing paradigm.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 1","pages":"Article 117100"},"PeriodicalIF":1.8,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Performance of the ELITe InGenius system for hepatitis B virus DNA quantification","authors":"Delphine Rosse ,&nbsp;Olivia Garrigou ,&nbsp;Valérie Ortonne ,&nbsp;Patrick Ingiliz ,&nbsp;Vincent Leroy ,&nbsp;Jean-Michel Pawlotsky ,&nbsp;Stéphane Chevaliez","doi":"10.1016/j.diagmicrobio.2025.117099","DOIUrl":"10.1016/j.diagmicrobio.2025.117099","url":null,"abstract":"<div><div>Measurement of hepatitis B virus (HBV) DNA levels is the standard of care for diagnosis active HBV infection, assessing disease severity and prognosis, and guiding treatment decisions and monitoring response to therapy. In the study, the analytical and clinical performance of the ELITe InGenius System for quantifying HBV DNA was evaluated. A total of 377 of archived EDTA plasma or serum specimens were tested. The HBV ELITe MGB Kit demonstrated linear quantification of HBV DNA with high precision. The specificity was 100 %. There was a satisfactory correlation with the cobas HBV Test, including patients on antiviral treatment with nucleos(t)ide analogues. The HBV ELITe MGB Kit using the ELITe InGenius System is suitable to detect and quantify HBV DNA in HBsAg-positive patients across the entire quantification dynamic range.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 1","pages":"Article 117099"},"PeriodicalIF":1.8,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145007772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of a commercial ELISA IgG antibody Kit for Orthoflavivirus nilense (West Nile Virus): Screening utility and comparison with virus neutralization test","authors":"Hasan Sercan Palancı ,&nbsp;Abdurrahman Gülmez ,&nbsp;Irmak Dik ,&nbsp;Oya Bulut ,&nbsp;Belgin Ünal ,&nbsp;İbrahim Mehmet Ali Öktem ,&nbsp;Özgen Alpay Özbek","doi":"10.1016/j.diagmicrobio.2025.117098","DOIUrl":"10.1016/j.diagmicrobio.2025.117098","url":null,"abstract":"<div><div>The diagnostic performance of a commercially available ELISA IgG antibody test for Orthoflavivirus nilense (ONV, formerly West Nile Virus) was evaluated using the virus neutralization (VN) test as the reference method. A total of 494 previously collected human serum samples were analyzed. Of the 40 ELISA IgG antibody positive samples, 38 were confirmed by VN, while VN detected 41 positive samples in total. The ELISA IgG antibody test demonstrated a sensitivity of 92.68 %, specificity of 99.56 %, positive predictive value of 95.00 %, and negative predictive value of 99.34 %, with an overall accuracy of 98.99 %. Receiver Operating Characteristic (ROC) analysis revealed a high discriminative performance (Area Under the Curve (AUC): 0.961). These results suggested that the ELISA IgG antibody test can serve as a rapid, economical, and automation-compatible screening assay for ONV antibody detection in outbreak settings and large-scale surveillance. However, due to potential cross-reactivity with antigenically related flaviviruses, all ELISA-reactive results must be confirmed by VN or plaque reduction neutralization test (PRNT) in compliance with World Organisation for Animal Health (WOAH) guidance to ensure virus-type–specific antibody identification.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 1","pages":"Article 117098"},"PeriodicalIF":1.8,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145039391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolated lumbar vertebral infection caused by Coxiella burnetii: A case report and literature review","authors":"Mingming Xin,&nbsp;Xiaohui Wang,&nbsp;Minggui Lin,&nbsp;Xuzhu Ma","doi":"10.1016/j.diagmicrobio.2025.117097","DOIUrl":"10.1016/j.diagmicrobio.2025.117097","url":null,"abstract":"<div><div>This study describes the clinical characteristics and treatment of vertebral infection caused by <em>Coxiella burnetii</em> through a case report and literature review. We present a 60-year-old male with isolated lumbar vertebral infection. A comprehensive literature review identified 17 cases, with 82.4 % of patients over 50 years old. Common symptoms included lumbar pain and fever, while 52.9 % developed adjacent vertebral infections through abdominal aortic aneurysms. Only 17.6 % presented as isolated vertebral infections. Radiological evidence of vertebral destruction was observed in all cases, with no cardiac valve involvement. Treatment involved doxycycline, hydroxychloroquine, fluoroquinolones, rifampicin, and surgical intervention, resulting in favorable outcomes. Isolated vertebral infections caused by <em>Coxiella burnetii</em> are extremely rare and pose significant diagnostic challenges. Early surgical intervention, effective diagnostic methods, and standardized anti-infective therapy are crucial for management. This case highlights the importance of timely diagnosis and multidisciplinary treatment for this rare condition.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 1","pages":"Article 117097"},"PeriodicalIF":1.8,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145010011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic value of Nanopore sequencing-based metagenomics next-generation sequencing in clinical infectious cases: A retrospective observational study","authors":"Yunqi Ji ,&nbsp;Jing Gu ,&nbsp;Shuang Xiao ,&nbsp;Manna Zhao ,&nbsp;Jie Xu ,&nbsp;Jing Lin ,&nbsp;Juanjuan Cui ,&nbsp;Weifeng Zhao","doi":"10.1016/j.diagmicrobio.2025.117092","DOIUrl":"10.1016/j.diagmicrobio.2025.117092","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aimed to evaluate the prognostic value of metagenomic next-generation sequencing(mNGS) using Nanopore sequencing technology (NST) versus traditional culture methods in infectious disease cases.</div></div><div><h3>Methods</h3><div>We conducted a retrospective, single-center observational study comparing clinical outcomes between patients and specimen types in NST group and those in culture-based control group. Cox Proportional Hazards regression and Kaplan-Meier survival analysis were conducted to evaluate the association between diagnostic strategy and 28-day mortality. To minimize bias, Propensity Score Matching (PSM) and robust multivariate Cox regression analysis were employed to control for confounding factors. Diagnostic accuracy was quantified by calculating sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV).</div></div><div><h3>Results</h3><div>The NST-based diagnosis was independently associated with a significantly reduced 28-day mortality rate, as indicated by the robust multivariate Cox regression analysis. (HR = 0.29, 95 %CI:0.09-0.91, p = 0.033). The sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of NST in detecting bacterium were 0.93,0.54, 0.5and 0.94, respectively. Additionally, the NST group was associated with higher Antibiotic De-escalation Rate (ADE) and reduced Duration of Therapy (DOT), with p values of 0.0238 and 0.008, respectively.</div></div><div><h3>Conclusion</h3><div>NST-based mNGS demonstrated superior performance in diagnosing bacterial infections compared to traditional culture methods. The NST group and positive pathogen detection were associated with a reduced 28-day mortality rate. Additionally, higher APACHE II scores upon admission were identified as independent predictors of worse prognosis, highlighting the importance of early severity assessment in patient outcomes.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 1","pages":"Article 117092"},"PeriodicalIF":1.8,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiological and genetic analysis of SEN virus infections in hemodialysis patients and healthy individuals in Tehran, Iran","authors":"Amin Naseri ,&nbsp;Enayat Anvari ,&nbsp;Seyyedeh Masumeh Mirnurollahi ,&nbsp;Abolfazl Fateh","doi":"10.1016/j.diagmicrobio.2025.117095","DOIUrl":"10.1016/j.diagmicrobio.2025.117095","url":null,"abstract":"<div><div>This case-control study investigated the epidemiological and genetic distribution of SEN virus (SENV) infections among 1,576 hemodialysis (HD) patients and 1,000 age- and gender-matched healthy individuals in Iran between 2017 and 2024. Nested PCR was utilized to amplify the ORF1 gene, facilitating the identification and sequencing of SENV genotypes D and H. The results demonstrated a significantly higher prevalence of SENV among HD patients (45.6%, 717/1,576) compared to healthy individuals (20.5%, 205/1,000) (<em>P</em> &lt; 0.001). Genotypic analysis showed that SENV-D (29.4% vs. 10.5%) and SENV-H (16.1% vs. 10.0%) were more frequently detected in HD patients. Additionally, co-infections with HBV (5.8% for SENV-D, 7.1% for SENV-H), HCV (28.4% for SENV-D, 45.1% for SENV-H, depending on genotype), and HIV (5.0% for SENV-D, 5.5% for SENV-H) were significantly associated with SENV infection in the HD group (<em>P</em> &lt; 0.001). In conclusion, this study provides valuable insights into the epidemiology of SENV in Iran, emphasizing the need for further research on its virological characteristics, transmission pathways, and potential interactions with co-infecting viruses to inform public health policies.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 1","pages":"Article 117095"},"PeriodicalIF":1.8,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145004845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A promising microscopic technique for detecting Schistosoma mansoni eggs","authors":"M.A Model,&nbsp;W.K. Anyan","doi":"10.1016/j.diagmicrobio.2025.117093","DOIUrl":"10.1016/j.diagmicrobio.2025.117093","url":null,"abstract":"<div><div>Schistosomiasis is one of the most devastating neglected tropical diseases, affecting over 250 million people worldwide and contributing to approximately 280,000 deaths annually. Microscopic detection of <em>Schistosoma mansoni</em> eggs in fecal samples using the Kato-Katz method remains the diagnostic standard. However, its speed and detection efficiency are limited. In this report, we present a novel microscopic technique - transmission-through-dye (TTD) imaging. TTD highlights viable eggs in bright red against a dark background, enhancing their visibility and detectability. TTD imaging appears to be a promising tool for the diagnosis of schistosomiasis.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 1","pages":"Article 117093"},"PeriodicalIF":1.8,"publicationDate":"2025-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144926302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Implementation of improved MALDI-TOF MS libraries for SBSEC subspecies identification in clinical practice","authors":"Schawanya Kaewpitoon Rattanapitoon,&nbsp;Nathkapach Kaewpitoon Rattanapitoon","doi":"10.1016/j.diagmicrobio.2025.117094","DOIUrl":"10.1016/j.diagmicrobio.2025.117094","url":null,"abstract":"<div><div>The recent article by Öberg et al. reports the creation and validation of novel MALDI-TOF MS libraries for <em>Streptococcus bovis</em>/<em>Streptococcus equinus</em>-complex (SBSEC) subspecies identification adapted to diagnostic culturing and extraction conditions. Their SBSEC<img>CMRS-BAE library demonstrated near-perfect identification performance, surpassing the Bruker MBT Compass and prior broth-based libraries. This letter highlights the strengths of their work, compares it with other MALDI-TOF MS database refinement studies, and discusses its immediate clinical applicability and limitations. We commend their development of a practical tool facilitating accurate SBSEC subspecies-level diagnostics, potentially improving endocarditis and colorectal cancer risk stratification in bacteremic patients.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 1","pages":"Article 117094"},"PeriodicalIF":1.8,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144989245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiological and molecular investigation of a norovirus GII.17 outbreak in a kindergarten in Shanghai, China","authors":"Jixing Yang ,&nbsp;Zhenzhen Qi ,&nbsp;Shuyang Chen ,&nbsp;Chenglong Xiong","doi":"10.1016/j.diagmicrobio.2025.117089","DOIUrl":"10.1016/j.diagmicrobio.2025.117089","url":null,"abstract":"<div><h3>Introduction</h3><div>Norovirus (NoV) is among the most prevalent pathogens responsible for non-bacterial acute gastroenteritis (AGE) in humans on a global scale.</div></div><div><h3>Methodology</h3><div>In September 2024, an epidemiological investigation and etiological analysis of a norovirus outbreak at a kindergarten in Shanghai Municipality of China was conducted to provide insights for preventing and controlling similar incidents in the future.</div></div><div><h3>Results</h3><div>A total of nine cases of vomiting and diarrhea were reported in the outbreak, involving eight students and one teacher in the same class. The onset of symptoms was concentrated from 26 to 28 September. Nucleic acid tests detected the presence of the norovirus GⅡ positive in the anal swab samples of three cases, and Genotyping confirmed the presence of the GII.17 norovirus. Furthermore, the virus strains were found to be highly homologous in the RdRp region and VP1 region of the gene. Evolutionary analysis showed that the isolates from this outbreak had high homology with the strains circulating in Europe and the United States in 2022-2024.</div></div><div><h3>Conclusions</h3><div>It is necessary to strengthen the research on the molecular evolution of VP1 region of norovirus GII.17 genotype to prevent the emergence of new variants that could cause serious damage.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 1","pages":"Article 117089"},"PeriodicalIF":1.8,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144933056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kerion in children caused by Trichophyton erinacei","authors":"Jingni Liang ,&nbsp;Rui Li ,&nbsp;Jing Zhao ,&nbsp;Fan Yang ,&nbsp;Tianhao Li ,&nbsp;Hongbin Cheng","doi":"10.1016/j.diagmicrobio.2025.117091","DOIUrl":"10.1016/j.diagmicrobio.2025.117091","url":null,"abstract":"<div><div>A 5-years-and 10-months old female presented with painful edematous plaques on the scalp for about 20 days, which worsened with pus for a week. The fungal fluorescent examination was positive, and <em>Trichophyton erinacei</em> was suggested by fungal culture and internal transcribed spacer (ITS). Finally, The patient was diagnosed with ‘Kerion celsi caused by <em>Trichophyton erinacei</em>’. After 8-weeks anti-infective and anti-fungal treatment, the patient’s skin lesions were healed with scars.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 1","pages":"Article 117091"},"PeriodicalIF":1.8,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144921675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}