Diagnostic microbiology and infectious disease最新文献_第4页

Phylogeography of SARS-CoV-2 Omicron sublineages detected in asymptomatic blood donors during third epidemiological wave in Mato Grosso, Midwestern Brazil.

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-01-18 DOI: 10.1016/j.diagmicrobio.2025.116693

Leonardo Marin , Lucas Santos de Miranda , Victor Hugo Silveira Carvalho , Maria Eduarda Fantacholi Voigt , João Pedro Lopes Martire , Márcio Roberto Teixeira Nunes , Renata Dezengrini Slhessarenko

{"title":"Phylogeography of SARS-CoV-2 Omicron sublineages detected in asymptomatic blood donors during third epidemiological wave in Mato Grosso, Midwestern Brazil.","authors":"Leonardo Marin , Lucas Santos de Miranda , Victor Hugo Silveira Carvalho , Maria Eduarda Fantacholi Voigt , João Pedro Lopes Martire , Márcio Roberto Teixeira Nunes , Renata Dezengrini Slhessarenko","doi":"10.1016/j.diagmicrobio.2025.116693","DOIUrl":"10.1016/j.diagmicrobio.2025.116693","url":null,"abstract":"<div><div>Emerging infectious disease agents represent pathogens that may evade current screening protocols while posing significant transfusion transmission risks regionally. This study investigated the prevalence of SARS-CoV-2 and other respiratory viruses among 633 blood donors at the MT-Hemocentro from November 2021 to February 2023. Nucleic acid obtained from nasopharyngeal swabs were tested by RT-qPCR for SARS-CoV-2, RSV, FLU-A, and FLU-B. Serum from positive samples was also tested for nucleic acid. The prevalence of SARS-CoV-2 was 6.48 % (41/633); 2 of the 41 blood donors had SARSCoV-2 detectable in their serum. All positive samples were collected between January 2022 and March 2023, coinciding with the third epidemic wave in Brazil; 97.6 % of these SARS-CoV-2-positive donors were vaccinated with at least two doses. SARS-CoV-2 genomes recovered from six nasopharyngeal samples were classified into BA.1.1.1, BA.1.14.1, BA.2, BA.5.1, BA.5.2.1 sublineages. Phylogeographic analysis across Brazil's five regions revealed that the Northeast acted as the main exporter of Omicron sublineages, while the South and Southeast regions were more frequently importers.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"111 3","pages":"Article 116693"},"PeriodicalIF":2.1,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143045966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whole genome sequencing and phylogenetic analysis to examine acquisition of Escherichia coli clones during international travel

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-01-18 DOI: 10.1016/j.diagmicrobio.2025.116701

Samantha dos S Tufic-Garutti , Luís G de A Longo , Roobinidevi Ragupathy , Maliha Akram , Mark C Enright , Beatriz M Moreira , Káris M de P Rodrigues

{"title":"Whole genome sequencing and phylogenetic analysis to examine acquisition of Escherichia coli clones during international travel","authors":"Samantha dos S Tufic-Garutti , Luís G de A Longo , Roobinidevi Ragupathy , Maliha Akram , Mark C Enright , Beatriz M Moreira , Káris M de P Rodrigues","doi":"10.1016/j.diagmicrobio.2025.116701","DOIUrl":"10.1016/j.diagmicrobio.2025.116701","url":null,"abstract":"<div><div>International travel facilitates the acquisition and carriage of extended-spectrum beta-lactamase-producing <em>Escherichia coli</em> (ESBL-E). We describe genomes of predominant ESBL-E clones detected before and after travel among subjects departing from Rio de Janeiro, Brazil, during 2015-2021, and genomes publicly available from countries visited by travelers. WGS (Illumina NovaSeq) was performed on 70 ESBL-E isolates from 66 travelers (18 pre- and 52 post-travel). Sequence type (ST), antimicrobial resistance (AMR), virulence genes, and plasmids were determined by Center for Genomic Epidemiology tools. Phylogenetic trees were constructed with each of the most frequent ST of travelers’ genomes (TG) and genomes with the same ST from Enterobase (EG). Other analyses were performed with Prokka, Roary, SNP-sites, and FastTree. Trees were visualized with iTOL. Among 70 ESBL-E, the clonal composition was quite diverse, with 41 different ST, with predominance before of CC10 and CC131, and after travel, CC10, CC131, CC69, and CC38, and three ST described for the first time: ST14408 and ST14412 (CC10), and ST4411 (CC20). Core genome phylogenetic analysis revealed 17 clusters, eight of which formed by post-travel TG and EG detected in the same country visited by traveler. We observed an increased number and diversity of AMR genes, plasmids, and virulence genes in post-travel isolates, although we only found statistical significance for IncFIB plasmid. Genome clustering supported the high-risk clone acquisition and AMR during international trips. More than half of detected clones were related to ExPEC and showed an increased number and diversity of AMR and virulence-related genes, as well as plasmids in post-travel isolates.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"111 3","pages":"Article 116701"},"PeriodicalIF":2.1,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143058384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mycobacterium tuberculosis DNA detection in formalin-fixed paraffin-embedded tissue using Digital PCR: a proof-of-concept study

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-01-17 DOI: 10.1016/j.diagmicrobio.2025.116697

Billie Velapatiño , Kerstin Locher , Corrie R Belanger , Marthe K Charles

{"title":"Mycobacterium tuberculosis DNA detection in formalin-fixed paraffin-embedded tissue using Digital PCR: a proof-of-concept study","authors":"Billie Velapatiño , Kerstin Locher , Corrie R Belanger , Marthe K Charles","doi":"10.1016/j.diagmicrobio.2025.116697","DOIUrl":"10.1016/j.diagmicrobio.2025.116697","url":null,"abstract":"<div><div>This proof-of-concept study aimed to assess whether digital PCR (dPCR) technology could improve the detection of <em>M. tuberculosis</em> complex (MTB) from formalin-fixed paraffin-embedded (FFPE) tissue when compared to real-time PCR. A laboratory developed test using real-time PCR assay targeting the MTB-IS<em>6110</em> was transitioned to dPCR technology. The analytical sensitivity of dPCR using strain H37Rv genomic DNA was 2.5 fg/reaction, which was lower than the 5 fg/reaction detected by real-time PCR. Archived DNA from 72 samples were evaluated retrospectively and compared to real-time PCR. The dPCR demonstrated 100 % (33/33) PPA and 94.7 % (38/39) NPA with real-time PCR. Thirteen samples with results near the limit of detection by real-time PCR were also at the limit of detection by dPCR. This study demonstrated that, while dPCR provided high analytical sensitivity, its performance for the MTB detection in FFPE tissues was comparable to that of real-time PCR. Both dPCR and real-time PCR are valuable tools for MTB detection especially in the absence of mycobacterial culture.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"111 3","pages":"Article 116697"},"PeriodicalIF":2.1,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143167265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence and risk factors evaluation for transfusion-transmissible infections among blood donors from Shiyan, China

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-01-17 DOI: 10.1016/j.diagmicrobio.2025.116691

Yanqing Zhao , Shuguo Yang , Shengnan Wei , Peng Wu , Yuting Li , Daiqian Zhu , Wenhao Li , Yao Zhang , Wei Wang , Danmei Jiao , Jian Li

{"title":"Prevalence and risk factors evaluation for transfusion-transmissible infections among blood donors from Shiyan, China","authors":"Yanqing Zhao , Shuguo Yang , Shengnan Wei , Peng Wu , Yuting Li , Daiqian Zhu , Wenhao Li , Yao Zhang , Wei Wang , Danmei Jiao , Jian Li","doi":"10.1016/j.diagmicrobio.2025.116691","DOIUrl":"10.1016/j.diagmicrobio.2025.116691","url":null,"abstract":"<div><h3>Objective</h3><div>Transfusion-transmissible infections (TTIs) are severe threats to blood safety and public health. A retrospective study of blood donor records from 2015 to 2019 in Shiyan, China, was conducted.</div></div><div><h3>Methods</h3><div>TTI prevalence was analyzed using ELISA, RT-PCR, and demographic data. Chi-square test and logistic regression were used to analyze incidence trends and associations for HIV, hepatitis B virus (HBV), hepatitis C virus (HCV), and <em>Treponema pallidum</em> (<em>T. pallidum</em>).</div></div><div><h3>Results</h3><div>Of 230,225 donors, 2,453 (1.07 %) were TTI-positive. HIV, HBV, HCV, syphilis rates were 0.08 %, 0.40 %, 0.19 %, 0.40 %. Significant decreases in HBV, HCV, <em>T. pallidum</em> from 2015 to 2019; HIV decreased from 2018 to 2019. The prevalence is higher among women aged 35–60, farmers with <em>T. pallidum</em> and young female students with HIV infection.</div></div><div><h3>Conclusions</h3><div>Despite the relatively low prevalence of TTIs at present, awareness of TTIs and ongoing blood screening are still needed to ensure blood safety.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"111 3","pages":"Article 116691"},"PeriodicalIF":2.1,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143037551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging and stirring Amoebic liver abscess in HIV seropositive men who have sex with men in South-Eastern China

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-01-17 DOI: 10.1016/j.diagmicrobio.2025.116696

Guanjing Lang , Weidong Ye , Guochun Chen , Tianye Zhu , Xiaofeng Li , Jinming Chen , Xingguo Miao , Feifei Su , Lijun Xu

{"title":"Emerging and stirring Amoebic liver abscess in HIV seropositive men who have sex with men in South-Eastern China","authors":"Guanjing Lang , Weidong Ye , Guochun Chen , Tianye Zhu , Xiaofeng Li , Jinming Chen , Xingguo Miao , Feifei Su , Lijun Xu","doi":"10.1016/j.diagmicrobio.2025.116696","DOIUrl":"10.1016/j.diagmicrobio.2025.116696","url":null,"abstract":"<div><div>Amebic liver abscess (ALA) is rare in Chinese population due to dietary habit. Nine Chinese HIV-seropositive men who have sex with men (MSM) with occurrence of amebic liver abscess (ALA) was retrospectively studied. Six participants had concurrent HIV/Syphilis and two had HIV/HCV. Symptoms primarily included fever, abdominal discomfort, and diarrhea. All participants showed intrahepatic abscesses, mainly in liver segments 6, 7, and 8, while two had additional extrahepatic amebic abscesses. Those with CD4 count < 100 cells/μL exhibited multiple and larger liver abscesses. Metagenomic Next-Generation Sequencing (mNGS) conclusively revealed <em>Entamoeba histolytica</em> despite bacterial culture and smear tests' negative results. All affected individuals were treated with percutaneous catheter drainage and metronidazole for at least 10 days. In summary, HIV-positive MSM are prone to develop ALA, and CD4 count < 100 cells/μL is a risk for occurrence of multiple and larger ALA. The optimal ALA treatment course for ALA remains dabatable.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"111 3","pages":"Article 116696"},"PeriodicalIF":2.1,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143167291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Balamuthia amoebic encephalitis survivor in China, and literature review

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-01-17 DOI: 10.1016/j.diagmicrobio.2025.116698

Yuemian Liang, Weina Wang

{"title":"A Balamuthia amoebic encephalitis survivor in China, and literature review","authors":"Yuemian Liang, Weina Wang","doi":"10.1016/j.diagmicrobio.2025.116698","DOIUrl":"10.1016/j.diagmicrobio.2025.116698","url":null,"abstract":"<div><div>Balamuthia amoebic encephalitis (BAE) is a rare, fatal parasitic infection of the central nervous system, with a current mortality rate above 95%. The high fatality rate is largely attributed to atypical clinicopathological features, delayed diagnosis, and the absence of effective treatment methods, so quick recognition of this disease is vital. In this paper, we present a survivor of BAE, who was confirmed through histologic examination and metagenomic next-generation sequencing (mNGS) of brain lesions. This case, unlike most previous reports, was a successful survival case. It highlights the critical need for differential diagnosis in patients with central nervous system infectious diseases, particularly those with a history of skin lesions and patients presenting multifocal brain lesions. Moreover, mNGS could serve as a useful tool in rapid identification of causative rare pathogens. The application of decompressive craniectomy may offer treatment opportunities and improve the survival rate of BAE. The case description was followed by a review of the literatures, in order to improve clinicians' understanding of this disease.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"111 3","pages":"Article 116698"},"PeriodicalIF":2.1,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peripheral blood lymphocyte subsets combined with fungal biomarkers in the diagnosis of invasive fungal infections complicated by sepsis 外周血淋巴细胞亚群与真菌生物标志物联合诊断侵袭性真菌感染合并败血症

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-01-16 DOI: 10.1016/j.diagmicrobio.2025.116694

Jinhua Zhang , Ye Jiang , Xiaoming Yao , Jianfeng Shi , Yueru Tian

{"title":"Peripheral blood lymphocyte subsets combined with fungal biomarkers in the diagnosis of invasive fungal infections complicated by sepsis","authors":"Jinhua Zhang , Ye Jiang , Xiaoming Yao , Jianfeng Shi , Yueru Tian","doi":"10.1016/j.diagmicrobio.2025.116694","DOIUrl":"10.1016/j.diagmicrobio.2025.116694","url":null,"abstract":"<div><h3>Objective</h3><div>To evaluate the lymphocyte subpopulations and the levels of (1, 3)-β-<span>d</span>-glucan (BDG) and galactomannan (GAL) in sepsis patients with secondary invasive fungal infections (IFIs), and to investigate the association between lymphocytes and IFIs.</div></div><div><h3>Methods</h3><div>A retrospective case-control study of 47 patients with sepsis complicated by IFIs, 50 sepsis patients, and 50 healthy controls was conducted. The correlation between lymphocyte subsets and BDG, GAL levels was analyzed using the Spearman correlation method, and the diagnostic efficacy of IFIs-related factors was assessed using the receiver operating characteristic curve (ROC) analysis.</div></div><div><h3>Results</h3><div>The IFIs group exhibited significantly lower absolute lymphocyte counts (<em>P</em> < 0.05). The study revealed that both BDG and GAL levels were inversely correlated with the counts of total lymphocytes and CD4+ lymphocytes (BDG: R<sup>2</sup> = 0.361, 0.283; GAL: R<sup>2</sup> =0.257, 0.375, <em>P</em> < 0.05). The area under the curve (AUC) for the combined diagnostic markers was as follows: BDG and GAL alone had an AUC of 0.753; BDG and GAL in conjunction with lymphocyte count had an AUC of 0.921; and the combination of BDG, GAL, lymphocyte count, and CD4 T lymphocyte count achieved an AUC of 0.962. The sensitivities for these combinations were 80.00 %, 80.85 %, and 87.23 %, respectively, while the specificities were 60.00 %, 96.0 %, and 98.00 %, respectively.</div></div><div><h3>Conclusions</h3><div>The reduced absolute counts of lymphocyte subsets in patients with IFIs complicated by sepsis suggest a state of immunosuppression. The concurrent assessment of BDG, GAL, total lymphocyte count, and CD4 T lymphocyte count demonstrates high diagnostic efficacy for identifying IFIs complicated by sepsis.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"111 3","pages":"Article 116694"},"PeriodicalIF":2.1,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterogeneity of OAS family expression in tuberculosis and the impact of different sample selection: a comprehensive analysis

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-01-16 DOI: 10.1016/j.diagmicrobio.2025.116692

Lingshan Dai, Bin Mei, Mingzhi Zhu, Hongjuan Zhou, Yanqin Shao, Lijun Peng

引用次数: 0

Antibiotics prescribing patterns before vs after minimum inhibitory concentration suppression: A quasi-experimental study

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-01-15 DOI: 10.1016/j.diagmicrobio.2025.116689

Huda J. Aljedaani , Manea F. Al Munjem , Mohammed Bazuqamah , Abrar K. Thabit

{"title":"Antibiotics prescribing patterns before vs after minimum inhibitory concentration suppression: A quasi-experimental study","authors":"Huda J. Aljedaani , Manea F. Al Munjem , Mohammed Bazuqamah , Abrar K. Thabit","doi":"10.1016/j.diagmicrobio.2025.116689","DOIUrl":"10.1016/j.diagmicrobio.2025.116689","url":null,"abstract":"<div><h3>Background</h3><div>Culture and susceptibility results are essential to optimize antibiotic treatment. Prescribers rely on minimum inhibitory concentration (MIC) interpretation to prescribe antibiotics. Many hospitals include MIC values with the interpretation in culture and susceptibility reports, where comparing MICs can be misleading (i.e., lower MIC doesn't necessarily suggest a more potent antibiotic). It is assumed that suppressing MIC values while keeping the interpretation may enhance the appropriate antibiotic choice. However, no study has evaluated the impact of such intervention. Therefore, we aimed to assess the impact of MIC suppression on appropriateness of antibiotic prescribing.</div></div><div><h3>Methods</h3><div>This was a quasi-experimental study over 12 months on patients with symptomatic infections confirmed by cultures and who were prescribed definitive antibiotics pre and post MIC suppression in culture reports. Endpoints included appropriateness of antibiotic and several clinical and economic outcomes.</div></div><div><h3>Results</h3><div>A total of 64 and 56 patients were included in the pre- and post-phase with median age of 57.5 vs. 62.3, respectively (<em>P</em> = 0.406). The appropriateness of antibiotic prescribing was higher in the post-phase (60.7 %), compared to the pre-phase (42.2 %) (<em>P</em> = 0.043). Hospital length of stay was lower in the post-phase compared to the pre-phase (7 vs. 10 days; <em>P</em> = 0.009). Consequently, the hospital stay cost was significantly lower in the post-phase than in the pre-phase ($3733 vs. $5333 <em>P</em> = 0.009).</div></div><div><h3>Conclusion</h3><div>Our study showed that suppressing MIC values in culture reports was associated with more appropriate antibiotic prescribing, as well as better clinical and economic outcomes. Microbiology labs should consider suppressing MICs as an antimicrobial stewardship intervention.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"111 3","pages":"Article 116689"},"PeriodicalIF":2.1,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of respiratory infections with the incidence and treatment efficacy in Kawasaki disease 川崎病呼吸道感染与发病率及治疗效果的关系。

IF 2.1 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-01-13 DOI: 10.1016/j.diagmicrobio.2025.116688

Soshi Hachisuka , Taito Kitano , Daisuke Kitagawa , Masayuki Murata , Mai Onishi , Takahiro Mori , Tenshin Okubo , Naohiro Yamamoto , Hiroki Nishikawa , Masayuki Onaka , Rika Suzuki , Madoka Sekine , Soma Suzuki , Fumihiko Nakamura , Sayaka Yoshida

{"title":"Association of respiratory infections with the incidence and treatment efficacy in Kawasaki disease","authors":"Soshi Hachisuka , Taito Kitano , Daisuke Kitagawa , Masayuki Murata , Mai Onishi , Takahiro Mori , Tenshin Okubo , Naohiro Yamamoto , Hiroki Nishikawa , Masayuki Onaka , Rika Suzuki , Madoka Sekine , Soma Suzuki , Fumihiko Nakamura , Sayaka Yoshida","doi":"10.1016/j.diagmicrobio.2025.116688","DOIUrl":"10.1016/j.diagmicrobio.2025.116688","url":null,"abstract":"<div><div>The etiology of Kawasaki disease (KD) has not yet been completely elucidated. We evaluated the temporal association between respiratory pathogens and KD incidence and the association between respiratory pathogens and treatment responsiveness in patients with KD.</div><div>This retrospective study was conducted to compare the number of symptomatic children with pathogens detected using multiplex PCR tests with the number of KD cases. Logistic analysis was performed to assess whether the presence of at least one respiratory virus was associated with immunoglobulin non-responsiveness in patients with KD. A statistically significant temporal association was found between the number of KD patients and the number of patients with a positive respiratory pathogen (Pearson correlation coefficient +0.595; <em>p</em>=0.003). The adjusted odds ratio of respiratory pathogen detection for immunoglobulin responsiveness was 1.24 (95% confidence interval 0.38–4.11) (<em>p</em>=0.721). Our study demonstrated a temporal association between the total number of respiratory infections and KD.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"111 3","pages":"Article 116688"},"PeriodicalIF":2.1,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0