Diagnostic microbiology and infectious disease最新文献_第5页

Cross-reactivity mediated by SARS-CoV-2 antibodies: Mechanism and impact of elevated HIV ELISA false-positive rates in the late-phase COVID-19 pandemic SARS-CoV-2抗体介导的交叉反应性：COVID-19大流行后期HIV ELISA假阳性率升高的机制及影响

IF 1.8 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-08-28 DOI: 10.1016/j.diagmicrobio.2025.117088

Wenhong Wang, Danxiao Wu, Xiaotao Li, Ziyun Du, Jinhui Liu, Yongjun Wang, Jie Dong

{"title":"Cross-reactivity mediated by SARS-CoV-2 antibodies: Mechanism and impact of elevated HIV ELISA false-positive rates in the late-phase COVID-19 pandemic","authors":"Wenhong Wang, Danxiao Wu, Xiaotao Li, Ziyun Du, Jinhui Liu, Yongjun Wang, Jie Dong","doi":"10.1016/j.diagmicrobio.2025.117088","DOIUrl":"10.1016/j.diagmicrobio.2025.117088","url":null,"abstract":"<div><div>This study analyzed the correlation between false-positive HIV ELISA results (using Bio-Rad reagents) and SARS-COV-2 antibody levels in 301 unpaid apheresis platelet donors with prior infection or vaccination, enrolled from Zhejiang Blood Center between February 1 and May 31, 2023. Trends in both the HIV ELISA false-positive rate and SARS-COV-2 antibody levels were assessed. The false-positive rate rose in early 2023, peaking at 0.68 % in mid-February, before gradually declining to the historical baseline of approximately 0.1 % by May's end. Similarly, donor SARS-COV-2 antibody levels peaked in February and subsequently decreased. Correlation analysis revealed a strong positive correlation (P<0.01) between HIV ELISA false-positive rates and SARS-CoV-2 antibody levels. The findings indicate a positive correlation, suggesting antibody cross-reactivity, likely mediated by structural similarities and shared epitopes between SARS-CoV-2 spike proteins and HIV-1 antigens (especially the p24 antigen in fourth-generation assays). The study recommends that significant fluctuations in false-positive rates warrant investigation into specific causes, including reagent issues. These findings underscore the need for continuous monitoring of false-positive results in HIV screening during pandemics. Improving reagent specificity and implementing rapid donor re-entry procedures are essential to safeguard blood supply efficiency and maintain donor trust.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 1","pages":"Article 117088"},"PeriodicalIF":1.8,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144989244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Disseminated mycobacterium colombiense infection associated with anti-interferon-γ autoantibody: a case report and literature review 与抗干扰素γ自身抗体相关的弥散性哥伦比亚分枝杆菌感染1例报告并文献复习

IF 1.8 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-08-28 DOI: 10.1016/j.diagmicrobio.2025.117087

Dan Zhou, Yi Xie, Yuling Xiao

引用次数: 0

Cover 2 - Aims/Scopes, Ed Board 封面2 -目标/范围，教育局

IF 1.8 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-08-28 DOI: 10.1016/S0732-8893(25)00404-3

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The first reported Crimean–Congo hemorrhagic fever reinfection in the literature: 11 years apart 文献中首次报道的克里米亚-刚果出血热再感染：相隔11年

IF 1.8 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-08-22 DOI: 10.1016/j.diagmicrobio.2025.117075

Seyit Ali Büyüktuna , Yasemin Çakır Kıymaz , Caner Öksüz , Murtaza Öz , Cihad Baysal , Nazif Elaldı

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Analysis of Predictive Factors for Mycoplasma-associated Plastic Bronchitis in Children 儿童支原体相关塑料支气管炎的预测因素分析

IF 1.8 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-08-22 DOI: 10.1016/j.diagmicrobio.2025.117073

Fengzhi Jiang, Xinlong Zhou, Huifang Zhang, Huihua Yuan, Xiaoxiu Li

{"title":"Analysis of Predictive Factors for Mycoplasma-associated Plastic Bronchitis in Children","authors":"Fengzhi Jiang, Xinlong Zhou, Huifang Zhang, Huihua Yuan, Xiaoxiu Li","doi":"10.1016/j.diagmicrobio.2025.117073","DOIUrl":"10.1016/j.diagmicrobio.2025.117073","url":null,"abstract":"<div><h3>Objective</h3><div>In recent years, the prevalence of Mycoplasma pneumoniae infection has led to complications such as mycoplasma-associated plastic bronchitis (M-PB) in some children. Therefore, it is particularly important to identify the predictive factors of M-PB occurrence and make early diagnosis and treatment.</div></div><div><h3>Method</h3><div>Analyze the pathogen of PB and collect 32 cases of M-PB and 60 cases of non-morphological mycoplasma pneumonia (N-PB) for comparative analysis.</div></div><div><h3>Result</h3><div>(1) A total of 126 cases of plastic bronchitis (PB) were collected, and the main pathogens were as follows: among them, 112 cases were combined with mycoplasma infection, 16 cases were combined with rhinovirus, 15 cases were combined with Streptococcus pneumoniae and Haemophilus influenzae respectively, 10 cases were combined with adenovirus, 5 cases were combined with bocavirus, 5 cases were combined with Moraxella catarrhalis, 3 cases were combined with Staphylococcus aureus, and 3 cases were combined with influenza A and B viruses respectively. (2) Binary Logistic regression analysis showed that the duration of fever and the ratio of neutrophils to lymphocytes (NLR) in blood were independent predictors of M-PB occurrence. The duration of fever (OR 1.525, 95% confidence interval: 1.112-2.089, P = 0.009), and NLR (OR 1.738, 95% confidence interval: 1.121-2.694, P = 0.013) (as shown in Table 3).</div></div><div><h3>Conclusion</h3><div>The main pathogen of plastic bronchitis is mycoplasma, and most of them are resistant to macrolide antibiotics. Other pathogens include rhinovirus, Streptococcus pneumoniae, Haemophilus influenzae and adenovirus. The duration of fever and NLR are independent predictive factors for the occurrence of mycoplasma-related plastic bronchitis.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 2","pages":"Article 117073"},"PeriodicalIF":1.8,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145120071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact on fetal medicine services of widespread parvovirus B19 outbreaks during 2024 2024年B19细小病毒大范围暴发对胎儿医学服务的影响

IF 1.8 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-08-22 DOI: 10.1016/j.diagmicrobio.2025.117079

Manjiri Khare , Farah Siddiqui , Suzanna Dunkerton , Eamonn Breslin , Hatem A Mousa , Ian Scudamore , Joshua Nazareth , Wael Goravey , Oliver TR Toovey , Julian W Tang

引用次数: 0

Higher detection rates of Mycoplasma pneumoniae in children with acute respiratory tract infections using oropharyngeal swabs compared to nasopharyngeal swabs 口咽拭子对急性呼吸道感染患儿肺炎支原体的检出率高于鼻咽拭子

IF 1.8 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-08-21 DOI: 10.1016/j.diagmicrobio.2025.117078

Yanfang Tan , Lidong Hua , Zengqing Li , Yuanping Tang , Zijun Guo , Liang Zhang , Hua Deng

{"title":"Higher detection rates of Mycoplasma pneumoniae in children with acute respiratory tract infections using oropharyngeal swabs compared to nasopharyngeal swabs","authors":"Yanfang Tan , Lidong Hua , Zengqing Li , Yuanping Tang , Zijun Guo , Liang Zhang , Hua Deng","doi":"10.1016/j.diagmicrobio.2025.117078","DOIUrl":"10.1016/j.diagmicrobio.2025.117078","url":null,"abstract":"<div><h3>Background</h3><div>Head-to-head comparisons of nasopharyngeal swabs (NPS) and oropharyngeal swabs (OPS) for microbiological analysis in pediatric patients with acute respiratory tract infections (ARTI) remain limited. This study aimed to evaluate the concordance of NPS and OPS in identifying various etiologies among children with ARTI.</div></div><div><h3>Methods</h3><div>Paired NPS and OPS samples were collected from 326 children with ARTI and analyzed for 15 respiratory pathogens using suspension microarray.</div></div><div><h3>Results</h3><div>NPS demonstrated a higher overall microbiological yield than OPS (79 % vs. 73 %, P <0.05). The five most frequently detected pathogens were respiratory syncytial virus, <em>Haemophilus influenzae, Mycoplasma pneumoniae</em> (MP), <em>Moraxella catarrhalis</em> (MC), and rhinovirus (RV). NPS exhibited significantly higher sensitivity for MC, RV, and parainfluenza viruses compared to OPS (P <0.01). Strikingly, MP detection rates were substantially higher in OPS than in NPS (84 % vs. 29 %, P <0.001).</div></div><div><h3>Conclusion</h3><div>While NPS generally yields better results for most pathogens, OPS is superior for MP detection. Combined sampling of NPS and OPS may reduce missed ARTI diagnoses in clinical practice.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 1","pages":"Article 117078"},"PeriodicalIF":1.8,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144933055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mixed respiratory infections in symptomatic outpatients: A study in community health settings 有症状门诊患者的混合呼吸道感染：社区卫生机构的一项研究

IF 1.8 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-08-20 DOI: 10.1016/j.diagmicrobio.2025.117074

Jiaman Liao , Xueliang Huang , Hao Huang , Cuina Shen , Jing Li , Haiting Zhai , Yiqiang Zhan , Lingwei Wang

{"title":"Mixed respiratory infections in symptomatic outpatients: A study in community health settings","authors":"Jiaman Liao , Xueliang Huang , Hao Huang , Cuina Shen , Jing Li , Haiting Zhai , Yiqiang Zhan , Lingwei Wang","doi":"10.1016/j.diagmicrobio.2025.117074","DOIUrl":"10.1016/j.diagmicrobio.2025.117074","url":null,"abstract":"<div><h3>Background</h3><div>Mixed respiratory infections in individuals with respiratory symptoms in primary care settings are understudied. This study aims to investigate their epidemiology and determinants.</div></div><div><h3>Methods</h3><div>A cross-sectional study was conducted with 244 consecutive adult patients with respiratory symptoms at Nanlian Community Health Center (January–March 2024). Throat swabs were analyzed for 18 respiratory pathogens and SARS-CoV-2 using multiplex PCR. Hematological parameters, including neutrophil-to-lymphocyte ratio (NLR), were measured from blood counts. The primary outcome was infection status (uninfected, single, or mixed). Multinomial logistic regression models identified factors associated with infection patterns.</div></div><div><h3>Results</h3><div>The initial cohort included 294 patients and 5,292 pathogen test results, with 319 (6.0 %) positive for at least one of 18 targeted pathogens. The most common pathogens in mixed infections were Influenza A Virus (25.1 %), H3N2 (23.5 %), and Haemophilus influenzae (22.3 %). After excluding 50 cases with incomplete questionnaires, the analytical cohort consisted of 244 participants (83.0 % of total), 56.1 % of whom were female. The largest age group was 19–40 years (71.3 %), and 65 participants (26.6 %) had mixed infections. Higher symptom counts (adjusted OR=1.700, 95 % CI 1.346–2.147) and elevated NLR (adjusted OR=1.232, 95 % CI 1.068–1.422) were linked to mixed infections. SARS-CoV-2 co-infection was negatively associated with mixed infections (adjusted OR=0.141, 95 % CI 0.035–0.575).</div></div><div><h3>Conclusions</h3><div>Mixed infections correlate with greater symptom burden and higher inflammation markers. The inverse relationship with SARS-CoV-2 warrants further investigation. Findings support targeted pathogen testing in symptomatic patients with elevated NLR.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 1","pages":"Article 117074"},"PeriodicalIF":1.8,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144890594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lectin based immunochromatographic assay for detection of kidney infections 基于凝集素的免疫层析检测肾脏感染

IF 1.8 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-08-20 DOI: 10.1016/j.diagmicrobio.2025.117076

Varduhi. A. Hovhannisyan , Inga L. Bazukyan , Vardan. K. Gasparyan

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Molecular analysis and ceftazidime–avibactam susceptibilities of carbapenem-resistant Klebsiella pneumoniae: Seven year experience from blood cultures of liver transplant recipients in a university hospital 碳青霉烯耐药肺炎克雷伯菌的分子分析和头孢他啶-阿维巴坦敏感性：一所大学医院肝移植受者血培养的7年经验

IF 1.8 4区医学

Diagnostic microbiology and infectious disease Pub Date : 2025-08-20 DOI: 10.1016/j.diagmicrobio.2025.117077

Elif Seren Tanriverdi , Yasar Bayindir , Yusuf Yakupogullari , Sibel Altunisik Toplu , Ertugrul Karabulut , Yucel Duman , Baris Otlu , Sezai Yilmaz

{"title":"Molecular analysis and ceftazidime–avibactam susceptibilities of carbapenem-resistant Klebsiella pneumoniae: Seven year experience from blood cultures of liver transplant recipients in a university hospital","authors":"Elif Seren Tanriverdi , Yasar Bayindir , Yusuf Yakupogullari , Sibel Altunisik Toplu , Ertugrul Karabulut , Yucel Duman , Baris Otlu , Sezai Yilmaz","doi":"10.1016/j.diagmicrobio.2025.117077","DOIUrl":"10.1016/j.diagmicrobio.2025.117077","url":null,"abstract":"<div><h3>Background</h3><div>Carbapenem-resistant <em>Klebsiella pneumoniae</em> (CRKP) infection is a major cause of mortality in liver transplantation (LT) recipients.</div></div><div><h3>Methods</h3><div>We aimed to elucidate the molecular mechanisms of carbapenem resistance among CRKP strains and determine ceftazidime-avibactam (CZA) <em>in vitro</em> susceptibility in high-risk patient populations, such as LT recipients.</div></div><div><h3>Results</h3><div>Of the 127 LT recipients between January 2016 and 2022, no statistically significant difference was found in the in vitro sensitivity of amikacin, gentamicin, ciprofloxacin, levofloxacin, imipenem, meropenem, piperacillin–tazobactam, trimethoprim–sulfamethoxazole, tigecycline, and colistin in patients with CRKP sepsis who survived and died at 30 days, except for amikacin and meropenem, which was statistically significant at 90 days (<em>p</em> ≤ 0.05). Of the isolates, 120 (94.4 %) were positive for the modified carbapenem inactivation test. When the carbapenemase genes of 127 CRKP isolates were analyzed, the most common carbapenemase gene was OXA-48 (59.1 %). The presence of OXA-48, OXA-48 + NDM, NDM, IMP, and VIM genes in CRKP isolates showed a statistically significant difference with the CZA susceptibility result (<em>p</em> ˂ 0.05).</div></div><div><h3>Conclusions</h3><div>The presence of the OXA-48 resistance gene in CRKP isolates was closely associated with <em>in vitro</em> susceptibility to CZA, regardless of the diversity of the patient population.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 1","pages":"Article 117077"},"PeriodicalIF":1.8,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144891943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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