Diagnostic microbiology and infectious disease最新文献

{"title":"Perinephritic and psoas abscess by an unusual coinfection with Trichomonas vaginalis and Lactobacillus johnsonii","authors":"Min Quan ,&nbsp;Xiaoxia Zhang ,&nbsp;Chao Chen ,&nbsp;Yu Feng ,&nbsp;Xiaoju Lv ,&nbsp;Xiaohui Wang ,&nbsp;Hui Ye","doi":"10.1016/j.diagmicrobio.2026.117276","DOIUrl":"10.1016/j.diagmicrobio.2026.117276","url":null,"abstract":"<div><div>Trichomoniasis, caused by <em>Trichomonas vaginalis,</em> is a common nonviral sexually transmitted infection that presents with vaginitis, urethritis, cystitis, prostatitis, and rarely perinephric abscess. Here we presented a 49-year-old female with fever and lumbago diagnosed with a coinfection of perinephric and psoas abscess caused by <em>Lactobacillus johnsonii</em> and <em>T. vaginalis</em>, with the etiological diagnosis established using pus culture and metagenomic next-generation sequencing (mNGS)<em>.</em> The patient achieved complete recovery following abscess drainage, incision, and combined therapy with metronidazole and piperacillin/tazobactam. Rare manifestations of trichomoniasis are easily misdiagnosed, and mNGS can help identify <em>T. vaginalis</em> quickly and accurately without prediction.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 4","pages":"Article 117276"},"PeriodicalIF":1.8,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146035253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neural network-enabled MALDI-TOF MS for rapid clinical identification of methicillin-resistant Staphylococcus aureus","authors":"Hengfang Shi ,&nbsp;Haiyong Wang ,&nbsp;Huiling Cao ,&nbsp;Junyao Sha ,&nbsp;Xiaofei Zhu","doi":"10.1016/j.diagmicrobio.2025.117250","DOIUrl":"10.1016/j.diagmicrobio.2025.117250","url":null,"abstract":"<div><h3>Objective</h3><div>To investigate the feasibility of utilizing neural network algorithm to analyze parameters from matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for rapid identification of methicillin-resistant <em>Staphylococcus aureus</em> (MRSA).</div></div><div><h3>Methods</h3><div>Strain identification was performed using MALDI-TOF MS, and antimicrobial susceptibility testing was conducted with the VITEK 2 Compact AST-GP639 card. Mass spectrometry parameters were collected from 41 <em>Staphylococcus aureus</em> strains isolated from different patients and anatomical sites, including 20 MRSA and 21 methicillin-susceptible <em>Staphylococcus aureus</em> (MSSA) isolates. Twenty characteristic mass spectral features were screened using LASSO regression analysis. A neural network model was established using these 20 mass spectrometry parameters to rapidly discriminate MRSA.</div></div><div><h3>Results</h3><div>LASSO identified 20 discriminatory m/z features, with individual AUCs ranging from 0.825 to 0.982. The ANN model, evaluated by leave-one-out cross-validation, achieved an overall accuracy of 97.56%, with 95% sensitivity and 100% specificity. Compared to single characteristic mass spectral features, the discrimination efficiency of the ANN model was significantly improved, while demonstrating high consistency with classical methods. In addition, SHapley Additive explanations analysis revealed that characteristic features e.g. m/z 147.7004 and 39,767.2735 played major roles in model predictions.</div></div><div><h3>Conclusion</h3><div>The neural network model based on MALDI-TOF MS parameters enables accurate discrimination between MRSA and MSSA. This approach offers rapid identification and cost-effectiveness, providing a novel strategy for clinical differentiation of MRSA and MSSA.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 4","pages":"Article 117250"},"PeriodicalIF":1.8,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145898006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The first reported case of combined occlusion of branch retinal artery and vein secondary to disseminated mycobacterium kansasii infection","authors":"Yanhua Luo ,&nbsp;Binwu Lin ,&nbsp;Baohua Luo ,&nbsp;Lin Qin ,&nbsp;Long Pang","doi":"10.1016/j.diagmicrobio.2025.117225","DOIUrl":"10.1016/j.diagmicrobio.2025.117225","url":null,"abstract":"<div><h3>Background</h3><div>Combined occlusion of branch retinal artery and vein in young patient is rare and indicates a high risk of underlying systemic disease. Retinal arteriovenous occlusion secondary to Mycobacterium kansasii (M. kansasii) infection has never been reported.</div></div><div><h3>Case presentation</h3><div>A 37-year-old woman, previously healthy and with nothing remarkable in her family history, presented to the ophthalmology clinic due to a 1-week history of blurred vision and inferior shadow of her right eye. She had been experiencing coughing with phlegm for one month prior to the eye symptoms. Upon examination, it was found that she had a simultaneous occlusion of branch retinal artery and branch retinal vein. Laboratory investigations revealed significant inflammation and hypercoagulability. Computed tomography revealed abnormally intense metabolic activity affecting the lungs, mediastinum, spleen, nasopharynx, neck, and bones. After three months, M. kansasii was identified through DNA sequencing of pathological tissue sections, leading to a diagnosis of disseminated M. kansasii infection. Although the medications (Rifampin, Isoniazid, Ethambutol, Clarithromycin) were administered according to standardized diagnostic and therapeutic guidelines as soon as the diagnosis was made, the patient was unable to complete the full course of medications due to her inability to tolerate the side effects of the medications. The patient's condition eventually worsened and resulted in death.</div></div><div><h3>Conclusions</h3><div>This is the first case of M. kansasii-associated hypercoagulability resulting in retinal arteriovenous occlusion. Disseminated Mycobacterium kansasii (DMK) infection is a rare but serious disease that can affect multiple organ systems. Rare retinal arteriovenous occlusion in young patients is often indicative of systemic disease and requires thorough evaluation to avoid misdiagnosis.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 4","pages":"Article 117225"},"PeriodicalIF":1.8,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145940956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"All spinal infections are not always tuberculosis: A rare case of brucella spondylodiscitis in South India","authors":"Venkat Narayana Goutham Valapala ,&nbsp;Prasanth Bylapudi ,&nbsp;Lakshmi Vasantha Poluri ,&nbsp;Mrudula Vandana ,&nbsp;Supriya Panda ,&nbsp;Pavani Dedeepya Singampalli ,&nbsp;Uday Kumar Yerramsetty ,&nbsp;Vijayalakshmi Payala","doi":"10.1016/j.diagmicrobio.2026.117262","DOIUrl":"10.1016/j.diagmicrobio.2026.117262","url":null,"abstract":"<div><div>A 49-year-old male presented with persistent fever for 20 days, accompanied by chills, headache, and vomiting. Medical history included congenital hearing impairment and speech difficulty, limiting communication; hence, the history was obtained from his wife. On examination, he was irritable, alert, and had neck stiffness without motor or sensory deficits. Cervical spine tenderness prompted a magnetic resonance imaging of the cervical spine, which demonstrated infective spondylodiscitis involving C6–C7. Given the endemic setting, multiple contiguous vertebral involvement, and occupational exposure as a butcher handling unvaccinated goats, differential diagnoses included tuberculous spondylitis and brucellosis. Blood cultures were performed from two sites; the second sample grew <em>Brucella melitensis</em>, identified using the VITEK 2 Compact system after incubation in the BACT/ALERT 3D system and subculture on blood agar. Treatment consisted of gentamicin, doxycycline, and rifampin. The patient became afebrile within 48 h and remained stable. He was discharged with instructions to complete the oral regimen, consisting of doxycycline and rifampin, and to wear a hard cervical collar.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 4","pages":"Article 117262"},"PeriodicalIF":1.8,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145959134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eighteen-year laboratory-based surveillance of human coronavirus OC43 in a single tertiary hospital in the Republic of Korea: Temporal inflection, seasonal stability, and age-dependent risk","authors":"Mi-Ru Oh ,&nbsp;Ga-Yeon Kim ,&nbsp;Jeong Su Han ,&nbsp;Jae Kyung Kim","doi":"10.1016/j.diagmicrobio.2026.117274","DOIUrl":"10.1016/j.diagmicrobio.2026.117274","url":null,"abstract":"<div><div>Human coronavirus OC43 (HCoV-OC43), a member of the betacoronavirus genus, is a seasonally circulating respiratory virus that persists globally; however, long-term laboratory-based surveillance data remain limited. We analyzed multiplex real-time PCR test results collected at Dankook University Hospital between 2007 and 2024 to comprehensively evaluate long-term annual trends, seasonal structure, and age-specific detection patterns of HCoV-OC43.</div><div>Based on nasopharyngeal swab specimens, the annual positivity rate of HCoV-OC43 ranged from 0.16% to 3.05%. A marked suppression in detection was observed during the COVID-19 pandemic (2020–2021) compared with the pre-pandemic years. This was followed by a partial recovery in 2022–2023; however, detection declined again in 2024 without fully returning to pre-pandemic levels. Seasonal analysis demonstrated a consistent winter predominance throughout the study, with the highest positivity observed in winter (4.44%) and the lowest in summer (1.16%), despite interannual variability. Age-specific analysis revealed highest positivity rates in early childhood 1–4 years (3.12%) and among infants aged 0 years (2.71%), followed by a progressive decline in detection across school-age children, adolescents, adults, and older adults, indicating a clear age-dependent distribution. These findings provide laboratory-based evidence characterizing the temporal, seasonal, and age-related detection patterns of HCoV-OC43 using long-term diagnostic data from a single institution. This information may serve as a reference for interpreting routine respiratory virus testing results and understanding seasonal diagnostic demand and surveillance strategies in clinical practice.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 4","pages":"Article 117274"},"PeriodicalIF":1.8,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145974571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular detection and phylogenetic analysis of KI polyomavirus and WU polyomavirus in respiratory tract samples from children under five","authors":"Hanife Tutan ,&nbsp;Yeşim Tok ,&nbsp;Mert Ahmet Kuşkucu","doi":"10.1016/j.diagmicrobio.2026.117272","DOIUrl":"10.1016/j.diagmicrobio.2026.117272","url":null,"abstract":"<div><h3>Background and Aim</h3><div>We aimed to investigate the frequency of KI and WU polyomavirus in respiratory tract samples, the affected groups by these viruses and their clinical characteristics. Although the viruses had been discovered in 2007, their pathogenicity and virulence have not been fully elucidated yet.</div></div><div><h3>Methods</h3><div>We examined 182 nasopharyngeal aspirate/swab samples obtained pediatric patients younger than five years and sent to our laboratory between 2016 and 2019. The viruses were investigated by nested-polymerase chain reaction using primers targeting partial VP1 gene regions. The positive samples were sequenced using Sanger method for verification and compare the genetic distances between partially amplified gene regions.</div></div><div><h3>Results</h3><div>KIPyV was detected in seven (3.8%) of 182 samples and WUPyV in two (1.1%). Except one KIPyV positive patient, all positive samples belonged to patients &lt;2 years of age. All positive patients were coinfected mainly by rhinovirus and parainfluenza virus 3. Four of the patients positive for KIPyV had haematologic/oncologic diseases or other immunodeficiency conditions. While all of the sequences of KIPyV isolates were 100% similar, sequences of WUPyV isolates differed at 4 nucleotide positions.</div></div><div><h3>Conclusion</h3><div>According to the results of our study, the frequency of KIPyV and WUPyV infections is low, simultaneous respiratory tract infections caused by other viral or bacterial pathogens, immunosuppression appears to be a predisposing factors for the KIPyV and WUPyV infections. The partially amplified gene regions were highly conserved in both viruses.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 4","pages":"Article 117272"},"PeriodicalIF":1.8,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145974572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative evaluation of BD MAX™ Enteric Panels and Luminex NxTAG® GPP for the detection of gastrointestinal pathogens in clinical stool samples","authors":"Javier Guillem ,&nbsp;Eliseo Albert ,&nbsp;Estela Giménez ,&nbsp;Isabel Corrales ,&nbsp;David Navarro ,&nbsp;Ignacio Torres","doi":"10.1016/j.diagmicrobio.2026.117267","DOIUrl":"10.1016/j.diagmicrobio.2026.117267","url":null,"abstract":"<div><h3>Purpose</h3><div>To compare the diagnostic performance of BD MAX™ Enteric Panels and the Luminex NxTAG® Gastrointestinal Pathogen Panel (GPP) for the detection of enteric pathogens under routine clinical conditions.</div></div><div><h3>Methods</h3><div>A prospective single-center study analyzed 165 stool samples (one per patient) from individuals with suspected gastrointestinal infection attending the Clínico-Malvarrosa Health Department (Valencia, Spain) between October and November 2023. Samples tested using all BD MAX™ enteric panels (bacterial, viral, and <em>Clostridioides difficile</em> panels) were also processed by Luminex NxTAG® GPP assay. Positive Percentage Agreement (PPA), Negative Percentage Agreement (NPA), Cohen’s κ index were calculated. Discrepant results were investigated using the VIASURE real-time PCR and targeted Next-Generation Sequencing, as appropriate.</div></div><div><h3>Results</h3><div>Among the 165 samples analyzed, 107 (64.8%) were positive for at least one pathogen by either method, with norovirus (<em>n</em> = 40) and <em>Clostridioides difficile</em> (<em>n</em> = 18) being the most frequently detected targets. Overall, both assays exhibited moderate initial agreement (PPA 69.3%, NPA 90.6%, κ = 0.56), with an average PPA of 78.6%. PPA exceeded 85% for most targets, except for sapovirus. <em>Salmonella</em> spp., and Shiga toxin-producing <em>E. coli</em> (stx1, stx2) (STEC) accounted for most discordant results, often associated with low pathogen loads or analytical variability. After discrepancy resolution, both assays demonstrated comparable diagnostic performance, with a substantial concordance of (κ = 0.79 and 0.77, respectively).</div></div><div><h3>Conclusion</h3><div>BD MAX™ and Luminex NxTAG® GPP demonstrated comparable accuracy in detecting enteric pathogens, supporting their use in cost-effective, culture-independent molecular diagnosis and their integration within microbiological diagnostic stewardship programs.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 4","pages":"Article 117267"},"PeriodicalIF":1.8,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145974575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive review of Candida spp./bacteria interactions within polymicrobial biofilms: resistance and therapeutic strategies","authors":"Amel Halimi ,&nbsp;Zahira Zakia Baba Ahmed-Kazi Tani ,&nbsp;Mohammed Aissaoui","doi":"10.1016/j.diagmicrobio.2026.117251","DOIUrl":"10.1016/j.diagmicrobio.2026.117251","url":null,"abstract":"<div><div>Fungal infections are mainly caused by opportunistic species of the <em>Candida</em> genus. These yeasts can form polymicrobial biofilms with bacteria on living or inert surfaces, including medical devices. The synergistic or antagonistic interactions within these biofilms promote virulence and resistance to antimicrobials as well as to the host's immune defenses. In response to this resistance, considerable efforts are being made to limit treatment failures. The use of antifungal-antibiotic-natural compound combinations, as well as the modification of medical device surfaces, offer promising solutions.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 4","pages":"Article 117251"},"PeriodicalIF":1.8,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145940957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and evaluation of a duplex real-time multienzyme isothermal rapid amplification assay for simultaneous detection of hypervirulent Klebsiella pneumoniae K1 and K2 serotypes in spiked blood samples","authors":"Zhixiong Duan ,&nbsp;Shaohong Yu ,&nbsp;Honglin Wang ,&nbsp;Chunyan Yang ,&nbsp;Xuelian Peng ,&nbsp;Shan Shi ,&nbsp;Jin Li","doi":"10.1016/j.diagmicrobio.2026.117280","DOIUrl":"10.1016/j.diagmicrobio.2026.117280","url":null,"abstract":"<div><div>The emergence of hypervirulent <em>Klebsiella pneumoniae</em> (hvKP), particularly the K1 and K2 serotypes, presents a significant public health threat due to their increased virulence and resistance. Rapid and accurate detection of hvKP is crucial for effective clinical management. This study developed and evaluated a duplex real-time multienzyme isothermal rapid amplification (MIRA) assay for the simultaneous detection of hvKP K1 and K2 serotypes in spiked blood samples. The assay employed optimized primers and probes targeting specific capsular genes. Sensitivity and specificity were assessed using spiked blood specimens and compared to real-time PCR. The detection limit was 1 × 10³ CFU per reaction for both serotypes, with no cross-reactivity with non-hvKP K1/K2 strains. The assay demonstrated superior reproducibility and stability, offering faster detection and simplified infrastructure requirements compared to real-time PCR. These features make the duplex real-time MIRA assay a promising tool for clinical diagnostics and outbreak surveillance.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 4","pages":"Article 117280"},"PeriodicalIF":1.8,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146017828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro antimicrobial susceptibility trends in commonly isolated anaerobic bacteria within the Australian context","authors":"Victoria Jordan ,&nbsp;Hemalatha Varadhan","doi":"10.1016/j.diagmicrobio.2026.117264","DOIUrl":"10.1016/j.diagmicrobio.2026.117264","url":null,"abstract":"<div><h3>Background</h3><div>Antibiotic susceptibility testing is often not performed for anaerobic bacteria; empiric antibiotics with anaerobic cover are used if indicated. However, resistance has been described to various commonly used anti-anaerobic agents, so it is important to assess whether this approach remains optimal.</div></div><div><h3>Aim</h3><div>To determine phenotypic susceptibility profiles of commonly isolated anaerobic bacteria.</div></div><div><h3>Methods</h3><div>Stored clinical isolates from within <em>Prevotella</em> spp. (n=30), <em>Clostridium perfringens</em> (n=30)<em>, Cutibacterium acnes</em> (n=30)<em>,</em> underwent susceptibility testing using EUCAST methodology; additionally gram positive anaerobic cocci (GPAC) such as <em>Finegoldia magna</em> (n=11)<em>, Parvimonas micra</em> (n=8)<em>,</em> and <em>Peptoniphilus</em> spp. (n=9) underwent susceptibility testing using an experimental method, the Thermo Fisher Anaerobe Sensititre broth microdilution plate.</div></div><div><h3>Results</h3><div>High resistance rates were found in <em>Prevotella</em> spp. to amoxicillin-clavulanate and clindamycin (53.3% and 50% respectively), as well as 43.3% clindamycin resistance in <em>Clostridium perfringens</em>, and notable clindamycin and tetracycline resistance rates in the gram positive anaerobic cocci<em>.</em> Susceptibility was generally otherwise retained for <em>F. necrophorum</em> and the Gram positive anaerobes tested, and to metronidazole for all organisms.</div></div><div><h3>Conclusion</h3><div>Given relatively high rates of resistance to common agents within <em>Prevotella</em> spp. and with clindamycin to several species tested, anaerobic susceptibility testing should be considered by laboratories in select circumstances, particularly in infections where <em>Prevotella</em> spp. have a significant role or where clindamycin is being considered as the main agent for significant infections.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 4","pages":"Article 117264"},"PeriodicalIF":1.8,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146009243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}