Higher detection rates of Mycoplasma pneumoniae in children with acute respiratory tract infections using oropharyngeal swabs compared to nasopharyngeal swabs

IF 1.8 4区医学 Q3 INFECTIOUS DISEASES

Diagnostic microbiology and infectious disease Pub Date : 2025-08-21 DOI:10.1016/j.diagmicrobio.2025.117078

Yanfang Tan , Lidong Hua , Zengqing Li , Yuanping Tang , Zijun Guo , Liang Zhang , Hua Deng

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引用次数: 0

Abstract

Background

Head-to-head comparisons of nasopharyngeal swabs (NPS) and oropharyngeal swabs (OPS) for microbiological analysis in pediatric patients with acute respiratory tract infections (ARTI) remain limited. This study aimed to evaluate the concordance of NPS and OPS in identifying various etiologies among children with ARTI.

Methods

Paired NPS and OPS samples were collected from 326 children with ARTI and analyzed for 15 respiratory pathogens using suspension microarray.

Results

NPS demonstrated a higher overall microbiological yield than OPS (79 % vs. 73 %, P <0.05). The five most frequently detected pathogens were respiratory syncytial virus, Haemophilus influenzae, Mycoplasma pneumoniae (MP), Moraxella catarrhalis (MC), and rhinovirus (RV). NPS exhibited significantly higher sensitivity for MC, RV, and parainfluenza viruses compared to OPS (P <0.01). Strikingly, MP detection rates were substantially higher in OPS than in NPS (84 % vs. 29 %, P <0.001).

Conclusion

While NPS generally yields better results for most pathogens, OPS is superior for MP detection. Combined sampling of NPS and OPS may reduce missed ARTI diagnoses in clinical practice.

查看原文本刊更多论文

口咽拭子对急性呼吸道感染患儿肺炎支原体的检出率高于鼻咽拭子

背景：在儿科急性呼吸道感染（ARTI）患者中，鼻咽拭子（NPS）和口咽拭子（OPS）用于微生物学分析的直接比较仍然有限。本研究旨在评估NPS和OPS在识别ARTI患儿各种病因方面的一致性。方法采集326例ARTI患儿的西班牙NPS和OPS样本，采用悬浮芯片检测15种呼吸道致病菌。结果snps的微生物总产率高于OPS （79% vs. 73%, P <0.05）。最常见的五种病原体是呼吸道合胞病毒、流感嗜血杆菌、肺炎支原体（MP）、卡他莫拉菌（MC）和鼻病毒（RV）。与OPS相比，NPS对MC、RV和副流感病毒的敏感性显著提高（P <0.01）。引人注目的是，OPS的MP检出率明显高于NPS（84%对29%，P <0.001）。结论NPS法对大多数病原菌的检测效果较好，而OPS法对MP的检测效果较好。NPS和OPS联合抽样可减少临床ARTI漏诊。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Diagnostic microbiology and infectious disease 医学-传染病学

CiteScore

5.30

自引率

3.40%

发文量

149

审稿时长

56 days

期刊介绍： Diagnostic Microbiology and Infectious Disease keeps you informed of the latest developments in clinical microbiology and the diagnosis and treatment of infectious diseases. Packed with rigorously peer-reviewed articles and studies in bacteriology, immunology, immunoserology, infectious diseases, mycology, parasitology, and virology, the journal examines new procedures, unusual cases, controversial issues, and important new literature. Diagnostic Microbiology and Infectious Disease distinguished independent editorial board, consisting of experts from many medical specialties, ensures you extensive and authoritative coverage.