DNA and cell biology最新文献_第8页

miRNA-382-5p Carried by Extracellular Vesicles in Osteoarthritis Reduces Cell Viability and Proliferation, and Promotes Cell Apoptosis by Targeting PTEN. 骨关节炎细胞外小泡携带的miRNA-382-5p通过靶向PTEN降低细胞活力和增殖，促进细胞凋亡

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-12-01 DOI: 10.1089/dna.2021.0726

Hanyu Lu, Yixin Yang, Shuanji Ou, Yong Qi, Guitao Li, Hebei He, Fanglian Lu, Wenjun Li, Hongtao Sun

{"title":"miRNA-382-5p Carried by Extracellular Vesicles in Osteoarthritis Reduces Cell Viability and Proliferation, and Promotes Cell Apoptosis by Targeting PTEN.","authors":"Hanyu Lu, Yixin Yang, Shuanji Ou, Yong Qi, Guitao Li, Hebei He, Fanglian Lu, Wenjun Li, Hongtao Sun","doi":"10.1089/dna.2021.0726","DOIUrl":"https://doi.org/10.1089/dna.2021.0726","url":null,"abstract":"The objective of the study was to identify extracellular vesicle (EV) microRNAs (miRNAs) that play important roles in knee osteoarthritis (OA). Models of knee OA were surgically induced in nine male Sprague-Dawley rats. Tissue samples were collected at 0 weeks (Control), 6 weeks (6 weeks), and 12 weeks (12 weeks). The EVs were isolated and analyzed for size. Various biomarkers, including recombinant tetraspanin 30 cluster of differentiation (CD)63 and CD9 were detected. An Agilent array was used to screen for differentially expressed (DE) miRNAs. The levels of DE miRNAs and their target mRNAs were evaluated by quantitative reverse transcription-polymerase chain reaction and western blotting. The viability, proliferation, and apoptosis of lipopolysaccharide (LPS)-induced human synovial cells (HSCs) were examined by using Cell Counting Kit-8, EdU (5-ethynyl-2'-deoxyuridine), and TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling) assays, respectively. The OA model rats had significantly increased levels of inflammatory activity, damaged cells, and rough articular cartilage when compared with rats in the control group. The EVs from the model rats appeared as round vesicle-like structures with a mean diameter of ∼145 nm. Five miRNAs that showed gradual increases in the model rats were selected for further analysis; those miRNAs included miR-127-3p, miR-132-3p, miR-141-3p, miR-345-5p, and miR-382-5p. miR-382-5p was found to reduce the viability and proliferation and promote the apoptosis of LPS-induced HSCs. Moreover, phosphatase and tensin homolog deleted on chromosome 10 (PTEN) was negatively regulated by miR-382-5p. Our findings revealed that EVs produced by the OA rats contained miR-382-5p, which might reduce cell viability and proliferation, and promote cell apoptosis by targeting PTEN.","PeriodicalId":11248,"journal":{"name":"DNA and cell biology","volume":"41 12","pages":"1012-1025"},"PeriodicalIF":3.1,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10402791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DosR Regulates the Transcription of the Arginine Biosynthesis Gene Cluster by Binding to the Regulatory Sequences in Mycobacterium bovis Bacille Calmette-Guerin. DosR通过结合牛分枝杆菌Calmette-Guerin调控序列调控精氨酸生物合成基因簇的转录。

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-12-01 DOI: 10.1089/dna.2022.0282

Yingying Cui, Guanghui Dang, Hui Wang, Yiyi Tang, Mingyue Lv, Xinxin Zang, Zhuming Cai, Ziyin Cui, Jun Cao, Siguo Liu, Ningning Song

{"title":"DosR Regulates the Transcription of the Arginine Biosynthesis Gene Cluster by Binding to the Regulatory Sequences in Mycobacterium bovis Bacille Calmette-Guerin.","authors":"Yingying Cui, Guanghui Dang, Hui Wang, Yiyi Tang, Mingyue Lv, Xinxin Zang, Zhuming Cai, Ziyin Cui, Jun Cao, Siguo Liu, Ningning Song","doi":"10.1089/dna.2022.0282","DOIUrl":"https://doi.org/10.1089/dna.2022.0282","url":null,"abstract":"l-Arginine serves as a carbon and nitrogen source and is critical for Mycobacterium tuberculosis (Mtb) survival in the host. Generally, ArgR acts as a repressor regulating arginine biosynthesis by binding to the promoter of the argCJBDFGH gene cluster. In this study, we report that the dormancy regulator DosR is a novel arginine regulator binding to the promoter region of argC (rv1652), which regulates arginine synthesis. Phosphorylation modification promoted DosR binding to a region upstream of the promoter. Cofactors, including arginine and metal ions, had an inhibitory effect on this association. Furthermore, DosR regulatory function relies on the interaction of the 167, 181, 182, and 197 amino acid residues with an inverse complementary sequence. Arginine also binds to DosR and directly affects its DNA-binding ability. Together, the results demonstrate that DosR acts as a novel transcriptional regulator of arginine synthesis in Mycobacterium bovis bacille Calmette-Guerin.","PeriodicalId":11248,"journal":{"name":"DNA and cell biology","volume":"41 12","pages":"1063-1074"},"PeriodicalIF":3.1,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10747672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Sp4 Regulates PTTG1IP Gene Transcription and Expression. Sp4调控PTTG1IP基因的转录和表达。

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-12-01 DOI: 10.1089/dna.2022.0243

Xi Dai, Shuyue Luo, Shipeng Guo, Weihui Zhou, Weihong Song

{"title":"Sp4 Regulates PTTG1IP Gene Transcription and Expression.","authors":"Xi Dai, Shuyue Luo, Shipeng Guo, Weihui Zhou, Weihong Song","doi":"10.1089/dna.2022.0243","DOIUrl":"https://doi.org/10.1089/dna.2022.0243","url":null,"abstract":"Pituitary tumor-transforming gene 1 protein (PTTG)-interacting protein, also known as PTTG-binding factor (PBF), is encoded by a proto-oncogene PTTG1IP. PBF has been identified through its interaction with PTTG. Similar to PTTG, PBF has been implicated in the etiology of several tumors, including pituitary, thyroid, and breast cancer. PBF can induce the translocation of PTTG into the nucleus, and then lead to tumorigenesis. Studies have shown that PBF plays a vital and complex role in increasing tumor development. However, the transcriptional regulation of PTTG1IP gene remains undefined. In this study, we have cloned a 467-bp fragment of the 5' flanking region of the human PTTG1IP gene and identified the region (-212 to +7 bp) necessary for PTTG1IP gene promoter activity by luciferase assay. Electrophoretic mobility shift assay revealed PTTG1IP gene promoter containing Sp4 response elements. Overexpression of Sp4 increased PTTG1IP gene transcription and expression in HeLa cells. Our study demonstrates that Sp4 regulates PTTG1IP gene transcription and expression.","PeriodicalId":11248,"journal":{"name":"DNA and cell biology","volume":"41 12","pages":"1053-1062"},"PeriodicalIF":3.1,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10389933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Psychotropic Drugs in the Discussion of Antimicrobial-Resistant Microorganisms. 精神药物与耐药微生物的讨论。

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-11-01 Epub Date: 2022-10-14 DOI: 10.1089/dna.2022.0471

Lori Ellezian, Archana Jhawar, Yasuhiro Kyono, Stephanie A Flowers

引用次数: 0

Genetic Variants of VDR and PGC-1α Are Not Associated with the Risk of Endometriosis in Indian Women. VDR和PGC-1α基因变异与印度女性子宫内膜异位症风险无关

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-11-01 Epub Date: 2022-10-14 DOI: 10.1089/dna.2022.0350

Himabindu Beeram, Swapna Siddamalla, Venkat Reddy Tumu, Veena Kv, Akanksha Vidala, Mamata Deenadayal, Shivaji Sisinthy, Manjula Bhanoori

{"title":"Genetic Variants of VDR and PGC-1α Are Not Associated with the Risk of Endometriosis in Indian Women.","authors":"Himabindu Beeram, Swapna Siddamalla, Venkat Reddy Tumu, Veena Kv, Akanksha Vidala, Mamata Deenadayal, Shivaji Sisinthy, Manjula Bhanoori","doi":"10.1089/dna.2022.0350","DOIUrl":"https://doi.org/10.1089/dna.2022.0350","url":null,"abstract":"An aberrant immunologic mechanism and mitochondrial biogenesis have been suggested to be involved in the pathogenesis of endometriosis. Genetic alterations in the vitamin D receptor (VDR) gene and peroxisome proliferator-activated receptor-gamma coactivator 1α (PGC-1α) may lead to important defects in gene activation, which principally affect immune function and normal mitochondrial function. Therefore, we hypothesized a possible role of VDR and PGC-1α genes in the pathogenesis of endometriosis and analyzed the association of genetic variants ApaI A/C (rs7975232) and TaqI T/C (rs731236) of VDR and rs8192678 (G/A), rs13131226 (T/C), and rs2970856 (T/C) of PGC-1α gene. This study included a total of 425 reproductive-age women (cases = 200 and controls = 225). Detection of VDR and PGC-1α gene polymorphism was performed using polymerase chain reaction-restriction fragment length polymorphism and sequencing analysis. The chi-square test was used to compare allele and genotype frequencies between groups, and a p-value of <0.05 was considered statistically significant. The genotype and allele distribution of both the gene polymorphisms did not show statistically significant association with endometriosis. Our result indicated ApaI A and TaqI T of VDR and GTT of PGC-1α gene as the most common haplotype in Indian women. The data suggest that VDR and PGC-1α gene polymorphisms did not play an important role in the pathogenesis of endometriosis in Indian women studied.","PeriodicalId":11248,"journal":{"name":"DNA and cell biology","volume":"41 11","pages":"987-995"},"PeriodicalIF":3.1,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33519337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitophagy: Critical Role in Atherosclerosis Progression. 线粒体自噬:动脉粥样硬化进展中的关键作用。

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-10-01 Epub Date: 2022-08-26 DOI: 10.1089/dna.2022.0249

Yanmei Chen, Wenhua Qin, Lu Li, Peng Wu, Dangheng Wei

引用次数: 3

A Brief Update for Our Readers. 给读者的简短更新。

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-09-01 Epub Date: 2022-07-12 DOI: 10.1089/dna.2022.29021.editorial

Carol Shoshkes Reiss

引用次数: 0

The Role of Alternative Splicing in Cancer: Regulatory Mechanism, Therapeutic Strategy, and Bioinformatics Application. 选择性剪接在癌症中的作用:调控机制、治疗策略和生物信息学应用。

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-09-01 Epub Date: 2022-08-10 DOI: 10.1089/dna.2022.0322

Yao-Jie Pan, Bo-Wen Liu, Dong-Sheng Pei

引用次数: 0

The Regulation of Autophagy by p38 MAPK-PPARγ Signaling During the Brain Ischemic Tolerance Induced by Cerebral Ischemic Preconditioning. 脑缺血预处理诱导脑缺血耐受过程中p38 MAPK-PPARγ信号对自噬的调控

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-09-01 Epub Date: 2022-08-09 DOI: 10.1089/dna.2022.0087

A-Chou Su, Ling-Yan Zhang, Jing-Ge Zhang, Yu-Yan Hu, Xi-Yun Liu, Shi-Chao Li, Xiao-Hui Xian, Wen-Bin Li, Min Zhang

{"title":"The Regulation of Autophagy by p38 MAPK-PPARγ Signaling During the Brain Ischemic Tolerance Induced by Cerebral Ischemic Preconditioning.","authors":"A-Chou Su, Ling-Yan Zhang, Jing-Ge Zhang, Yu-Yan Hu, Xi-Yun Liu, Shi-Chao Li, Xiao-Hui Xian, Wen-Bin Li, Min Zhang","doi":"10.1089/dna.2022.0087","DOIUrl":"https://doi.org/10.1089/dna.2022.0087","url":null,"abstract":"Several studies indicated that autophagy activation participates in brain ischemic tolerance (BIT) induced by cerebral ischemic preconditioning (CIP). However, the mechanism of autophagy activation during the process still remains unclear. The present study aimed to evaluate the role of p38 MAPK-peroxisome proliferator-activated receptor γ (PPARγ) signaling cascade in autophagy during the CIP-induced BIT. The results shown that, initially, autophagy activation was observed after CIP in the model of global cerebral ischemia in rats, as was indicated by the upregulation of Beclin 1 expression, an increase in LC3-II/LC3-I ratio, the enhanced LC3 immunofluorescence, and a rise in the number of autophagosomes in the neurons of the hippocampal CA1 area. Besides, the inhibitor of autophagy 3-methyladenine obliterated the neuroprotection induced by CIP. Furthermore, the upregulation of p-p38 MAPK and PPARγ expressions was earlier than autophagy activation after CIP. In addition, pretreatment with SB203580 (the inhibitor of p38 MAPK) reversed CIP-induced PPARγ upregulation, autophagy activation, and neuroprotection. Pretreatment with GW9662 (the inhibitor of PPARγ) reversed autophagy activation and neuroprotection, while it had no effect on p-p38 MAPK upregulation induced by CIP. These data suggested that the p38 MAPK-PPARγ signaling pathway participates in autophagy activation during the induction of BIT by CIP.","PeriodicalId":11248,"journal":{"name":"DNA and cell biology","volume":"41 9","pages":"838-849"},"PeriodicalIF":3.1,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40613553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Epigenetic Control of Vascular Smooth Muscle Cell Function in Atherosclerosis: A Role for DNA Methylation. 动脉粥样硬化中血管平滑肌细胞功能的表观遗传控制:DNA甲基化的作用。

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-09-01 Epub Date: 2022-07-27 DOI: 10.1089/dna.2022.0278

Yanjun Chen, Lingli Liang, Chunyan Wu, Zitong Cao, Linzhen Xia, Jun Meng, Zuo Wang

{"title":"Epigenetic Control of Vascular Smooth Muscle Cell Function in Atherosclerosis: A Role for DNA Methylation.","authors":"Yanjun Chen, Lingli Liang, Chunyan Wu, Zitong Cao, Linzhen Xia, Jun Meng, Zuo Wang","doi":"10.1089/dna.2022.0278","DOIUrl":"https://doi.org/10.1089/dna.2022.0278","url":null,"abstract":"Atherosclerosis is a complex vascular inflammatory disease in which multiple cell types are involved, including vascular smooth muscle cells (VSMCs). In response to vascular injury and inflammatory stimuli, VSMCs undergo a \"phenotypic switching\" characterized by extracellular matrix secretion, loss of contractility, and abnormal proliferation and migration, which play a key role in the progression of atherosclerosis. DNA methylation modification is an important epigenetic mechanism that plays an important role in atherosclerosis. Studies investigating abnormal DNA methylation in patients with atherosclerosis have determined a specific DNA methylation profile, and proposed multiple pathways and genes involved in the etiopathogenesis of atherosclerosis. Recent studies have also revealed that DNA methylation modification controls VSMC function by regulating gene expression involved in atherosclerosis. In this review, we summarize the recent advances regarding the epigenetic control of VSMC function by DNA methylation in atherosclerosis and provide insights into the development of VSMC-centered therapeutic strategies.","PeriodicalId":11248,"journal":{"name":"DNA and cell biology","volume":"41 9","pages":"824-837"},"PeriodicalIF":3.1,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40639015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1