DNA and cell biology最新文献_第8页

miRNA-382-5p Carried by Extracellular Vesicles in Osteoarthritis Reduces Cell Viability and Proliferation, and Promotes Cell Apoptosis by Targeting PTEN. 骨关节炎细胞外小泡携带的miRNA-382-5p通过靶向PTEN降低细胞活力和增殖，促进细胞凋亡

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-12-01 DOI: 10.1089/dna.2021.0726

Hanyu Lu, Yixin Yang, Shuanji Ou, Yong Qi, Guitao Li, Hebei He, Fanglian Lu, Wenjun Li, Hongtao Sun

{"title":"miRNA-382-5p Carried by Extracellular Vesicles in Osteoarthritis Reduces Cell Viability and Proliferation, and Promotes Cell Apoptosis by Targeting PTEN.","authors":"Hanyu Lu, Yixin Yang, Shuanji Ou, Yong Qi, Guitao Li, Hebei He, Fanglian Lu, Wenjun Li, Hongtao Sun","doi":"10.1089/dna.2021.0726","DOIUrl":"https://doi.org/10.1089/dna.2021.0726","url":null,"abstract":"The objective of the study was to identify extracellular vesicle (EV) microRNAs (miRNAs) that play important roles in knee osteoarthritis (OA). Models of knee OA were surgically induced in nine male Sprague-Dawley rats. Tissue samples were collected at 0 weeks (Control), 6 weeks (6 weeks), and 12 weeks (12 weeks). The EVs were isolated and analyzed for size. Various biomarkers, including recombinant tetraspanin 30 cluster of differentiation (CD)63 and CD9 were detected. An Agilent array was used to screen for differentially expressed (DE) miRNAs. The levels of DE miRNAs and their target mRNAs were evaluated by quantitative reverse transcription-polymerase chain reaction and western blotting. The viability, proliferation, and apoptosis of lipopolysaccharide (LPS)-induced human synovial cells (HSCs) were examined by using Cell Counting Kit-8, EdU (5-ethynyl-2'-deoxyuridine), and TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling) assays, respectively. The OA model rats had significantly increased levels of inflammatory activity, damaged cells, and rough articular cartilage when compared with rats in the control group. The EVs from the model rats appeared as round vesicle-like structures with a mean diameter of ∼145 nm. Five miRNAs that showed gradual increases in the model rats were selected for further analysis; those miRNAs included miR-127-3p, miR-132-3p, miR-141-3p, miR-345-5p, and miR-382-5p. miR-382-5p was found to reduce the viability and proliferation and promote the apoptosis of LPS-induced HSCs. Moreover, phosphatase and tensin homolog deleted on chromosome 10 (PTEN) was negatively regulated by miR-382-5p. Our findings revealed that EVs produced by the OA rats contained miR-382-5p, which might reduce cell viability and proliferation, and promote cell apoptosis by targeting PTEN.","PeriodicalId":11248,"journal":{"name":"DNA and cell biology","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10402791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sp4 Regulates PTTG1IP Gene Transcription and Expression. Sp4调控PTTG1IP基因的转录和表达。

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-12-01 DOI: 10.1089/dna.2022.0243

Xi Dai, Shuyue Luo, Shipeng Guo, Weihui Zhou, Weihong Song

引用次数: 0

Identification of Diagnostic Variants in FGFR2 and NPR2 Genes in a Chinese Family Affected by Crouzon Syndrome and Acromesomelic Dysplasia, Type Maroteaux. 中国Crouzon综合征和Maroteaux型端端粒发育不良家族FGFR2和NPR2基因诊断变异的鉴定

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-11-01 Epub Date: 2022-11-02 DOI: 10.1089/dna.2022.0453

JianJiang Zhu, Ran Meng, HuaWei Zhao, LiRong Cai, XiaoHui Wen, Wen Zeng, Yao Luo, Hong Qi

{"title":"Identification of Diagnostic Variants in FGFR2 and NPR2 Genes in a Chinese Family Affected by Crouzon Syndrome and Acromesomelic Dysplasia, Type Maroteaux.","authors":"JianJiang Zhu, Ran Meng, HuaWei Zhao, LiRong Cai, XiaoHui Wen, Wen Zeng, Yao Luo, Hong Qi","doi":"10.1089/dna.2022.0453","DOIUrl":"https://doi.org/10.1089/dna.2022.0453","url":null,"abstract":"This study aims to conduct a comprehensive clinical and genetic investigation on a large family with members having various phenotypes, including acromesomelic dysplasia, type Maroteaux (AMDM), idiopathic short stature (ISS), Crouzon syndrome (CS). Prenatal diagnosis was performed on the high-risk fetus. We performed the whole-exome sequencing on three members with AMDM, ISS, or CS. Detailed genotypes and phenotypes were investigated on members of this 4-generation family. Genetic analysis identified three variants, which were designated as p.Val548del, p.Arg989Gln in natriuretic peptide receptor B/guanylate cyclase B (NPR2), and p.Cys342Tyr in fibroblast growth factor receptor-2 (FGFR2). Compound heterozygous variation consisting of p.Val548del and p.Arg989Gln caused AMDM. NPR2 heterozygous variant carriers exhibited normal height or ISS. The p.Cys342Tyr mutation of FGFR2 causes the typical clinical phenotype of CS. The fetus carried the heterozygous p.Val548del and p.Cys342Tyr mutations, with ultrasound results showing exophthalmos, parrot-beaked nose, low and flat frontal skull, and intrauterine growth retardation at the second and third trimesters of gestation. We are reporting those two novel mutations (p.Val548del and p.Arg989Gln) in NPR2 and a p.Cys342Tyr mutation in FGFR2 in an extended Chinese family. This finding extended the genotype-phenotype spectra of ISS, AMDM, and CS related to pathogenic variants.","PeriodicalId":11248,"journal":{"name":"DNA and cell biology","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40661113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances of Mesenchymal Stem Cells Released Extracellular Vesicles in Periodontal Bone Remodeling. 间充质干细胞释放细胞外囊泡在牙周骨重塑中的研究进展。

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-11-01 Epub Date: 2022-10-28 DOI: 10.1089/dna.2022.0359

Chaoran Liu, Yanan Li, Guanghong Han

{"title":"Advances of Mesenchymal Stem Cells Released Extracellular Vesicles in Periodontal Bone Remodeling.","authors":"Chaoran Liu, Yanan Li, Guanghong Han","doi":"10.1089/dna.2022.0359","DOIUrl":"https://doi.org/10.1089/dna.2022.0359","url":null,"abstract":"Extracellular vesicles (EVs) are nanoparticles that include exosomes, microvesicles, and apoptotic bodies; they interact with target cell surface receptors and transport contents, including mRNA, proteins, and enzymes into the cytoplasm of target cells to function. The biological fingerprints of EVs practically mirror those of the parental cells they originated from. In the bone remodeling microenvironment, EVs could act on osteoblasts to regulate the bone formation, promote osteoclast differentiation, and regulate bone resorption. Therefore, there have been many attempts wherein EVs were used to achieve targeted therapy in bone-related diseases. Periodontitis, a common bacterial infectious disease, could cause severe alveolar bone resorption, resulting in tooth loss, whereas research on periodontal bone regeneration is also an urgent question. Therefore, EVs-related studies are important for periodontal bone remodeling. In this review, we summarize the current knowledge of mesenchymal stem cell-EVs involved in periodontal bone remodeling and explore the functional gene expression through a comparative analysis of transcriptomic content.","PeriodicalId":11248,"journal":{"name":"DNA and cell biology","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40673451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Psychotropic Drugs in the Discussion of Antimicrobial-Resistant Microorganisms. 精神药物与耐药微生物的讨论。

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-11-01 Epub Date: 2022-10-14 DOI: 10.1089/dna.2022.0471

Lori Ellezian, Archana Jhawar, Yasuhiro Kyono, Stephanie A Flowers

引用次数: 0

Quantitative Data-Independent Acquisition Mass Spectrometry Proteomics and Weighted Correlation Network Analysis of Plasma Samples for the Discovery of Chronic Kidney Disease-Specific Atherosclerosis Risk Factors. 血浆样本的定量数据独立获取质谱蛋白质组学和加权相关网络分析用于发现慢性肾脏疾病特异性动脉粥样硬化危险因素。

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-11-01 Epub Date: 2022-10-17 DOI: 10.1089/dna.2022.0200

Daopeng Dai, Zhiwei Cheng, Shuo Feng, Zhengbin Zhu, Jiwei Yu, Wenli Zhang, Hui Lu, Ruiyan Zhang, Jinzhou Zhu

{"title":"Quantitative Data-Independent Acquisition Mass Spectrometry Proteomics and Weighted Correlation Network Analysis of Plasma Samples for the Discovery of Chronic Kidney Disease-Specific Atherosclerosis Risk Factors.","authors":"Daopeng Dai, Zhiwei Cheng, Shuo Feng, Zhengbin Zhu, Jiwei Yu, Wenli Zhang, Hui Lu, Ruiyan Zhang, Jinzhou Zhu","doi":"10.1089/dna.2022.0200","DOIUrl":"https://doi.org/10.1089/dna.2022.0200","url":null,"abstract":"Chronic kidney disease (CKD) accelerates atherosclerosis. The mechanism of CKD-related atherosclerosis is complex, and CKD-specific risk factors may contribute to this process in addition to traditional risk factors such as hypertension, diabetes, and hypercholesterolemia. In the present study, to discover CKD-specific atherosclerosis risk factors, a total of 62 patients with different stages of kidney function were enrolled. All patients underwent coronary angiographies and the severity of coronary atherosclerosis was defined by the SYNTAX score. Patients were divided into different groups according to their kidney function levels and coronary atherosclerosis severity. Data-independent acquisition mass spectrometry was used to identify differentially expressed proteins (DEPs) in the plasma samples, and weighted correlation network analysis (WGCNA) was employed to identify significant protein modules and hub proteins related to CKD-specific atherosclerosis. The results showed that 10 DEPs associated with atherosclerosis were found in the comparative groups with modest and severe CKD. Through WGCNA, 1768 proteins were identified and 8 protein modules were established. Enrichment analyses of protein modules revealed functional clusters mainly associated with inflammation and the complement and coagulation cascade as atherosclerosis developed under CKD conditions. The results may help to better understand the mechanisms of CKD-related atherosclerosis and guide future research on developing treatments for CKD-related atherosclerosis.","PeriodicalId":11248,"journal":{"name":"DNA and cell biology","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40338812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation Between the MTHFR C677T Genotype and Coronary Heart Disease in Populations from Gansu, China. 甘肃人群中MTHFR C677T基因型与冠心病的相关性

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-11-01 Epub Date: 2022-10-26 DOI: 10.1089/dna.2022.0329

Xue Wu, Kai Liu, Xinke Zhao, Xiaowei Zhang, Huan Guo, Hugang Jiang, Juan Chang, Xinfang Lv, Xiang Gao, Xiaodong Zhi, Chunzhen Ren, Qilin Chen, Yufang Liang, Yingdong Li

{"title":"Correlation Between the MTHFR C677T Genotype and Coronary Heart Disease in Populations from Gansu, China.","authors":"Xue Wu, Kai Liu, Xinke Zhao, Xiaowei Zhang, Huan Guo, Hugang Jiang, Juan Chang, Xinfang Lv, Xiang Gao, Xiaodong Zhi, Chunzhen Ren, Qilin Chen, Yufang Liang, Yingdong Li","doi":"10.1089/dna.2022.0329","DOIUrl":"https://doi.org/10.1089/dna.2022.0329","url":null,"abstract":"This study was designed to evaluate the relationship between polymorphisms in the methylenetetrahydrofolate reductase (MTHFR) gene and coronary heart disease (CHD) in populations from the Gansu region of China. The MTHFR C677T polymorphism genotypes from 209 patients with CHD, as confirmed by coronary angiography, and 212 non-CHD control patients were identified using PCR gold magnetic particle chromatography. We simultaneously evaluated homocysteine (Hcy) and folate levels in these samples using biochemical methods. The TT genotype of the MTHFR C677T locus was significantly more frequent in the CHD group than in the control, while the CC genotype was significantly less frequent in CHD patients than in non-CHD patients (p < 0.05). In addition, biochemical analysis revealed that the serum Hcy levels increased, and folate levels decreased in the TT genotype. Logistic regression analysis showed that this correlation was independent of nationality, sex, age, body mass index, medical history, and blood lipid level (p < 0.05). The occurrence of the TT genotype at the MTHFR C677T locus was closely associated with CHD in the Gansu population and may serve as a biomarker of increased risk for this disease.","PeriodicalId":11248,"journal":{"name":"DNA and cell biology","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40666337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Endoplasmic Reticulum Stress and Oxidative Stress in Inflammatory Diseases. 炎症性疾病中的内质网应激和氧化应激。

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-11-01 Epub Date: 2022-09-14 DOI: 10.1089/dna.2022.0353

Yun Tang, Xiangping Zhou, Ting Cao, En Chen, Yumeng Li, Wenbo Lei, Yibao Hu, Bisha He, Shuangquan Liu

引用次数: 9

Genetic Variants of VDR and PGC-1α Are Not Associated with the Risk of Endometriosis in Indian Women. VDR和PGC-1α基因变异与印度女性子宫内膜异位症风险无关

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-11-01 Epub Date: 2022-10-14 DOI: 10.1089/dna.2022.0350

Himabindu Beeram, Swapna Siddamalla, Venkat Reddy Tumu, Veena Kv, Akanksha Vidala, Mamata Deenadayal, Shivaji Sisinthy, Manjula Bhanoori

{"title":"Genetic Variants of VDR and PGC-1α Are Not Associated with the Risk of Endometriosis in Indian Women.","authors":"Himabindu Beeram, Swapna Siddamalla, Venkat Reddy Tumu, Veena Kv, Akanksha Vidala, Mamata Deenadayal, Shivaji Sisinthy, Manjula Bhanoori","doi":"10.1089/dna.2022.0350","DOIUrl":"https://doi.org/10.1089/dna.2022.0350","url":null,"abstract":"An aberrant immunologic mechanism and mitochondrial biogenesis have been suggested to be involved in the pathogenesis of endometriosis. Genetic alterations in the vitamin D receptor (VDR) gene and peroxisome proliferator-activated receptor-gamma coactivator 1α (PGC-1α) may lead to important defects in gene activation, which principally affect immune function and normal mitochondrial function. Therefore, we hypothesized a possible role of VDR and PGC-1α genes in the pathogenesis of endometriosis and analyzed the association of genetic variants ApaI A/C (rs7975232) and TaqI T/C (rs731236) of VDR and rs8192678 (G/A), rs13131226 (T/C), and rs2970856 (T/C) of PGC-1α gene. This study included a total of 425 reproductive-age women (cases = 200 and controls = 225). Detection of VDR and PGC-1α gene polymorphism was performed using polymerase chain reaction-restriction fragment length polymorphism and sequencing analysis. The chi-square test was used to compare allele and genotype frequencies between groups, and a p-value of <0.05 was considered statistically significant. The genotype and allele distribution of both the gene polymorphisms did not show statistically significant association with endometriosis. Our result indicated ApaI A and TaqI T of VDR and GTT of PGC-1α gene as the most common haplotype in Indian women. The data suggest that VDR and PGC-1α gene polymorphisms did not play an important role in the pathogenesis of endometriosis in Indian women studied.","PeriodicalId":11248,"journal":{"name":"DNA and cell biology","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33519337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential of Mesenchymal Stem Cell-Based Therapies for Pulmonary Fibrosis. 间充质干细胞治疗肺纤维化的潜力。

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-11-01 Epub Date: 2022-10-26 DOI: 10.1089/dna.2022.0327

Zhihou Guo, Yaping Zhang, Furong Yan

{"title":"Potential of Mesenchymal Stem Cell-Based Therapies for Pulmonary Fibrosis.","authors":"Zhihou Guo, Yaping Zhang, Furong Yan","doi":"10.1089/dna.2022.0327","DOIUrl":"https://doi.org/10.1089/dna.2022.0327","url":null,"abstract":"Pulmonary fibrosis (PF) is a common pathological feature of acute and chronic inflammatory lung diseases that currently has no effective clinical treatment. Mesenchymal stem cells (MSCs) are considered to be an ideal cell source for regenerating injured tissues, as they are easily extracted and expanded, have a limited risk of tumorigenicity, and lack immunogenicity. Recently, MSC-based therapies have been suggested as potential therapeutic strategies for attenuating PF. Although the administration of MSCs has been reported to have anti-inflammatory and anti-fibrotic effects in PF, further studies are required to optimize the MSC source and dose, delivery time, and route of administration to improve their protective effects. Moreover, the mechanisms underlying MSC-based therapies for PF remain elusive. Here, we review recently published data on MSC administration for the treatment of PF to provide insights into the therapeutic impact of MSC delivery procedures and sources. In addition, we discuss the potential mechanisms underlying the effects of MSC administration on PF and highlight promising strategies for improving the beneficial effects of MSCs.","PeriodicalId":11248,"journal":{"name":"DNA and cell biology","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40666338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3