DosR Regulates the Transcription of the Arginine Biosynthesis Gene Cluster by Binding to the Regulatory Sequences in Mycobacterium bovis Bacille Calmette-Guerin.

IF 2.6 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Yingying Cui, Guanghui Dang, Hui Wang, Yiyi Tang, Mingyue Lv, Xinxin Zang, Zhuming Cai, Ziyin Cui, Jun Cao, Siguo Liu, Ningning Song
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引用次数: 1

Abstract

l-Arginine serves as a carbon and nitrogen source and is critical for Mycobacterium tuberculosis (Mtb) survival in the host. Generally, ArgR acts as a repressor regulating arginine biosynthesis by binding to the promoter of the argCJBDFGH gene cluster. In this study, we report that the dormancy regulator DosR is a novel arginine regulator binding to the promoter region of argC (rv1652), which regulates arginine synthesis. Phosphorylation modification promoted DosR binding to a region upstream of the promoter. Cofactors, including arginine and metal ions, had an inhibitory effect on this association. Furthermore, DosR regulatory function relies on the interaction of the 167, 181, 182, and 197 amino acid residues with an inverse complementary sequence. Arginine also binds to DosR and directly affects its DNA-binding ability. Together, the results demonstrate that DosR acts as a novel transcriptional regulator of arginine synthesis in Mycobacterium bovis bacille Calmette-Guerin.

DosR通过结合牛分枝杆菌Calmette-Guerin调控序列调控精氨酸生物合成基因簇的转录。
l-精氨酸作为碳源和氮源,对结核分枝杆菌(Mtb)在宿主体内的存活至关重要。一般来说,ArgR通过结合argCJBDFGH基因簇的启动子来调节精氨酸的生物合成。在这项研究中,我们报道了休眠调节剂DosR是一种新的精氨酸调节剂,结合到argC (rv1652)的启动子区域,调节精氨酸的合成。磷酸化修饰促进DosR结合到启动子上游的一个区域。辅助因子,包括精氨酸和金属离子,对这种关联有抑制作用。此外,DosR的调控功能依赖于167、181、182和197个氨基酸残基与一个反向互补序列的相互作用。精氨酸也与DosR结合并直接影响其dna结合能力。综上所述,DosR是牛分枝杆菌Calmette-Guerin中精氨酸合成的一种新的转录调节因子。
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来源期刊
DNA and cell biology
DNA and cell biology 生物-生化与分子生物学
CiteScore
6.60
自引率
0.00%
发文量
93
审稿时长
1.5 months
期刊介绍: DNA and Cell Biology delivers authoritative, peer-reviewed research on all aspects of molecular and cellular biology, with a unique focus on combining mechanistic and clinical studies to drive the field forward. DNA and Cell Biology coverage includes: Gene Structure, Function, and Regulation Gene regulation Molecular mechanisms of cell activation Mechanisms of transcriptional, translational, or epigenetic control of gene expression Molecular Medicine Molecular pathogenesis Genetic approaches to cancer and autoimmune diseases Translational studies in cell and molecular biology Cellular Organelles Autophagy Apoptosis P bodies Peroxisosomes Protein Biosynthesis and Degradation Regulation of protein synthesis Post-translational modifications Control of degradation Cell-Autonomous Inflammation and Host Cell Response to Infection Responses to cytokines and other physiological mediators Evasive pathways of pathogens.
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