Sp4调控PTTG1IP基因的转录和表达。

IF 2.6 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

DNA and cell biology Pub Date : 2022-12-01 DOI:10.1089/dna.2022.0243

Xi Dai, Shuyue Luo, Shipeng Guo, Weihui Zhou, Weihong Song

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引用次数: 0

摘要

垂体肿瘤转化基因1蛋白(PTTG)相互作用蛋白，也称为PTTG结合因子(PBF)，由原癌基因PTTG1IP编码。PBF是通过与PTTG的相互作用确定的。与PTTG类似，PBF与几种肿瘤的病因有关，包括垂体癌、甲状腺癌和乳腺癌。PBF可诱导PTTG易位进入细胞核，进而导致肿瘤发生。研究表明，PBF在促进肿瘤发展中起着重要而复杂的作用。然而，PTTG1IP基因的转录调控尚不明确。在这项研究中，我们克隆了人类PTTG1IP基因5'侧区467 bp的片段，并通过荧光素酶测定鉴定了PTTG1IP基因启动子活性所需的区域(-212至+7 bp)。电泳迁移率转移分析显示PTTG1IP基因启动子含有Sp4响应元件。Sp4过表达增加了HeLa细胞中PTTG1IP基因的转录和表达。我们的研究表明Sp4调控PTTG1IP基因的转录和表达。

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Sp4 Regulates PTTG1IP Gene Transcription and Expression.

Pituitary tumor-transforming gene 1 protein (PTTG)-interacting protein, also known as PTTG-binding factor (PBF), is encoded by a proto-oncogene PTTG1IP. PBF has been identified through its interaction with PTTG. Similar to PTTG, PBF has been implicated in the etiology of several tumors, including pituitary, thyroid, and breast cancer. PBF can induce the translocation of PTTG into the nucleus, and then lead to tumorigenesis. Studies have shown that PBF plays a vital and complex role in increasing tumor development. However, the transcriptional regulation of PTTG1IP gene remains undefined. In this study, we have cloned a 467-bp fragment of the 5' flanking region of the human PTTG1IP gene and identified the region (-212 to +7 bp) necessary for PTTG1IP gene promoter activity by luciferase assay. Electrophoretic mobility shift assay revealed PTTG1IP gene promoter containing Sp4 response elements. Overexpression of Sp4 increased PTTG1IP gene transcription and expression in HeLa cells. Our study demonstrates that Sp4 regulates PTTG1IP gene transcription and expression.

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来源期刊

DNA and cell biology 生物-生化与分子生物学

CiteScore

6.60

自引率

0.00%

发文量

审稿时长

1.5 months

期刊介绍： DNA and Cell Biology delivers authoritative, peer-reviewed research on all aspects of molecular and cellular biology, with a unique focus on combining mechanistic and clinical studies to drive the field forward. DNA and Cell Biology coverage includes: Gene Structure, Function, and Regulation Gene regulation Molecular mechanisms of cell activation Mechanisms of transcriptional, translational, or epigenetic control of gene expression Molecular Medicine Molecular pathogenesis Genetic approaches to cancer and autoimmune diseases Translational studies in cell and molecular biology Cellular Organelles Autophagy Apoptosis P bodies Peroxisosomes Protein Biosynthesis and Degradation Regulation of protein synthesis Post-translational modifications Control of degradation Cell-Autonomous Inflammation and Host Cell Response to Infection Responses to cytokines and other physiological mediators Evasive pathways of pathogens.