DNA and cell biology最新文献_第10页

The Tumor Suppressor Roles and Mechanisms of MiR-491 in Human Cancers. MiR-491在人类癌症中的抑瘤作用和机制

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-09-01 Epub Date: 2022-08-01 DOI: 10.1089/dna.2022.0274

Farzad Sadri, Seyede Fatemeh Hosseini, Atena Aghayei, Mohammad Fereidouni, Zohreh Rezaei

引用次数: 2

Epigenetic Control of Vascular Smooth Muscle Cell Function in Atherosclerosis: A Role for DNA Methylation. 动脉粥样硬化中血管平滑肌细胞功能的表观遗传控制:DNA甲基化的作用。

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-09-01 Epub Date: 2022-07-27 DOI: 10.1089/dna.2022.0278

Yanjun Chen, Lingli Liang, Chunyan Wu, Zitong Cao, Linzhen Xia, Jun Meng, Zuo Wang

{"title":"Epigenetic Control of Vascular Smooth Muscle Cell Function in Atherosclerosis: A Role for DNA Methylation.","authors":"Yanjun Chen, Lingli Liang, Chunyan Wu, Zitong Cao, Linzhen Xia, Jun Meng, Zuo Wang","doi":"10.1089/dna.2022.0278","DOIUrl":"https://doi.org/10.1089/dna.2022.0278","url":null,"abstract":"Atherosclerosis is a complex vascular inflammatory disease in which multiple cell types are involved, including vascular smooth muscle cells (VSMCs). In response to vascular injury and inflammatory stimuli, VSMCs undergo a \"phenotypic switching\" characterized by extracellular matrix secretion, loss of contractility, and abnormal proliferation and migration, which play a key role in the progression of atherosclerosis. DNA methylation modification is an important epigenetic mechanism that plays an important role in atherosclerosis. Studies investigating abnormal DNA methylation in patients with atherosclerosis have determined a specific DNA methylation profile, and proposed multiple pathways and genes involved in the etiopathogenesis of atherosclerosis. Recent studies have also revealed that DNA methylation modification controls VSMC function by regulating gene expression involved in atherosclerosis. In this review, we summarize the recent advances regarding the epigenetic control of VSMC function by DNA methylation in atherosclerosis and provide insights into the development of VSMC-centered therapeutic strategies.","PeriodicalId":11248,"journal":{"name":"DNA and cell biology","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40639015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Dihydroartemisinin Attenuates Hypoxic Pulmonary Hypertension via the Downregulation of miR-335 Targeting Vangl2. 双氢青蒿素通过下调靶向Vangl2的miR-335减轻低氧性肺动脉高压。

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-08-01 Epub Date: 2022-07-19 DOI: 10.1089/dna.2021.1113

Yaozhe Li, Haijian Cai, Jinqiu Wei, Lin Zhu, Yizhu Yao, Mengyao Xie, Lanlan Song, Chi Zhang, Xiaoying Huang, Liangxing Wang

{"title":"Dihydroartemisinin Attenuates Hypoxic Pulmonary Hypertension via the Downregulation of miR-335 Targeting Vangl2.","authors":"Yaozhe Li, Haijian Cai, Jinqiu Wei, Lin Zhu, Yizhu Yao, Mengyao Xie, Lanlan Song, Chi Zhang, Xiaoying Huang, Liangxing Wang","doi":"10.1089/dna.2021.1113","DOIUrl":"https://doi.org/10.1089/dna.2021.1113","url":null,"abstract":"Dihydroartemisinin (DHA) is a traditional antimalarial drug. DHA plays a crucial role in preventing pulmonary hypertension (PH); however, its regulatory function on microRNAs (miRNAs) in PH remains unclear. This study aimed to investigate whether DHA exerts its protective functions by regulating miR-335 in PH. Hypoxia-induced PH models were induced both in vitro and in vivo. Mice were treated with various concentrations of DHA, and pulmonary arterial smooth muscle cells (PASMCs) were treated with DHA, miR-335 inhibitor, miR-335 mimic, or Van Gogh-like 2 (Vangl2) plasmid. The expression of miR-335 and Vangl2, pulmonary arterial remodeling index; right ventricular hypertrophy index; and proliferation and migration indexes were measured. DHA improved pulmonary vascular remodeling and alleviated PH in vivo. miRNA sequencing and real-time PCR results further show that the increase in hypoxia-induced miR-335 was avoided by DHA administration, and miR-335 increased the hypoxia-induced PASMC proliferation and migration. MiRNA databases and dual-luciferase reporter assay show that miR-335 directly targets Vangl2, and Vangl2 decreased the hypoxia-induced PASMC proliferation and migration. The miR-335 inhibitor failed to inhibit hypoxia-induced proliferation and migration upregulation in Vangl2 knockdown PASMCs, and the effect of DHA can be blocked by miR-335 upregulation. In hypoxic PH, MiR-335 is increased, whereas Vangl2 is decreased. MiR-335 can significantly promote the hypoxia-induced proliferation and migration of PASMCs by targeting the Vangl2 gene. DHA effectively reverses the hypoxia-induced upregulation of miR-335 expression, avoiding the miR-335-mediated downregulation of Vangl2 and thereby promoting the expression of Vangl2 to prevent PH.","PeriodicalId":11248,"journal":{"name":"DNA and cell biology","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40525651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Evaluation of Hematologic Parameters in Patients with COVID-19 Following Mesenchymal Stem Cell Therapy. 间充质干细胞治疗后COVID-19患者血液学参数的评价

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-08-01 DOI: 10.1089/dna.2021.1198

Haniye Bakhshi, Masoud Soleimani, Mina Soufizomorrod, Omid Kooshkaki

引用次数: 0

High Levels of Organochlorines Are Associated with Induction of ABL1 Promoter Methylation in Children with Acute Lymphoblastic Leukemia. 高水平的有机氯与急性淋巴细胞白血病儿童ABL1启动子甲基化的诱导相关

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-08-01 Epub Date: 2022-07-05 DOI: 10.1089/dna.2022.0232

Arash Rafeeinia, Gholamreza Asadikaram, Mehrnaz Karimi-Darabi, Vahid Moazed

{"title":"High Levels of Organochlorines Are Associated with Induction of ABL1 Promoter Methylation in Children with Acute Lymphoblastic Leukemia.","authors":"Arash Rafeeinia, Gholamreza Asadikaram, Mehrnaz Karimi-Darabi, Vahid Moazed","doi":"10.1089/dna.2022.0232","DOIUrl":"https://doi.org/10.1089/dna.2022.0232","url":null,"abstract":"Exposure to organochlorines is associated with epigenetic changes, including methylation change in the promoter of tumor suppressor genes, thereby leading to cancer induction. The aim of this study was to investigate the relationship between organochlorine pesticides (OCPs) and ABL1 promoter methylation in child patients with acute lymphoblastic leukemia (ALL) and the control group. The methylation rate of the ABL1 promoter was evaluated using the methylation-specific polymerase chain reaction method, and the level of OCPs in patients with ALL and healthy children was measured using gas chromatography. ABL1 promoter hypermethylation was observed in 64% of ALL patients and 28.5% of children in the control group. The level of OCPs in children with methylated ABL1 promoters was significantly higher than that in children with nonmethylated ABL1 promoters (p < 0.05). Our findings suggest that OCPs, especially alpha-hexachlorocyclohexane, beta-hexachlorocyclohexane, gamma-hexachlorocyclohexane, 2,4 dichlorodiphenyldichloroethylene, and 4,4 dichlorodiphenyltrichloroethane may induce methylation at the ABL1 promoter level, thereby preventing the normal expression of the ABL1 gene. As a result, the reduced expression of ABL1 (a tumor suppressor) gene due to the hypermethylation of its promoter leads to the disruption of normal biological processes, thus making cells vulnerable to oncogenic factors.","PeriodicalId":11248,"journal":{"name":"DNA and cell biology","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40472239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

4'-Fluorouridine Is a Broad-Spectrum Orally Available First-Line Antiviral That May Improve Pandemic Preparedness. 4′-氟吡啶是一种广谱口服一线抗病毒药物，可改善大流行防备。

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-08-01 DOI: 10.1089/dna.2022.0312

Carolin M Lieber, Richard K Plemper

{"title":"4'-Fluorouridine Is a Broad-Spectrum Orally Available First-Line Antiviral That May Improve Pandemic Preparedness.","authors":"Carolin M Lieber, Richard K Plemper","doi":"10.1089/dna.2022.0312","DOIUrl":"https://doi.org/10.1089/dna.2022.0312","url":null,"abstract":"The COVID-19 pandemic has highlighted the urgent need for the development of broad-spectrum antivirals to enhance preparedness against future spillover of zoonotic viruses with pandemic potential into the human population. Currently, the direct-acting orally available SARS-CoV-2 inhibitors molnupiravir and paxlovid are approved for human use under emergency use authorization. A promising next-generation therapeutic candidate is the orally available ribonucleoside analog 4'-fluorouridine (4'-FlU) that had potent antiviral efficacy against different viral targets, including SARS-CoV-2 in human organoids and animal models. Although a nucleoside analog inhibitor such as molnupiravir that targets the viral RNA-dependent RNA polymerase (RdRP) complex, 4'-FlU showed a distinct mechanism of activity, delayed chain termination, compared with molnupiravir's induction of viral error catastrophe. This review will focus on some currently approved and emerging medicines developed against SARS-CoV-2, examining their potential to form a pharmacological first-line defense against zoonotic viruses with pandemic potential.","PeriodicalId":11248,"journal":{"name":"DNA and cell biology","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9416544/pdf/dna.2022.0312.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9914729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Proliferating Stem Cells are Acutely Affected by DNA Damage Induced by Sulfur Mustard. 硫芥对增殖干细胞DNA损伤的影响

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-08-01 Epub Date: 2022-07-13 DOI: 10.1089/dna.2022.0073

Zengming Zhao, Xiabei Yan, Lizhong Li, Yulei Shu, Jun He, Lili Wang, Qingzhen Huang, Jianwei Xie, Jun Zhao, Shuangqing Peng

引用次数: 2

MicroRNA-26b Reduces Cell Viability by Inhibition of Nicotinamide Phosphoribosyltransferase in Breast Cancer Cells. MicroRNA-26b通过抑制乳腺癌细胞烟酰胺磷酸核糖基转移酶降低细胞活力

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-08-01 Epub Date: 2022-07-06 DOI: 10.1089/dna.2022.0214

Melika Ameli Mojarad, Mandana Ameli Mojarad, Alireza Pourmahdian

{"title":"MicroRNA-26b Reduces Cell Viability by Inhibition of Nicotinamide Phosphoribosyltransferase in Breast Cancer Cells.","authors":"Melika Ameli Mojarad, Mandana Ameli Mojarad, Alireza Pourmahdian","doi":"10.1089/dna.2022.0214","DOIUrl":"https://doi.org/10.1089/dna.2022.0214","url":null,"abstract":"Breast cancer (BC) is one of the most common causes of cancer in women worldwide and it is found to be associated with an increased level of Nicotinamide phosphoribosyltransferase (NAMPT), which plays an important role in nicotinamide adenine dinucleotide (NAD) pathway, both in blood and tumor tissues. This enzyme is also essential for the growth and survival of cancer cells. The short noncoding RNA microRNAs miR-26b is an important gene regulator and a tumor suppressor in different human cancers, including BC. In this study, bioinformatics analysis was evaluated to find the miRNAs targeting NAMPT 3' untranslated regions (3' UTRs), which was confirmed by luciferase assay. Next, we evaluate NAMPT and microRNA-26b (miR-26b) expression by using polymerase chain reaction (PCR) in BC. miR-26b effect on cell viability was also evaluated by Cell Counting Kit-8 (CCK-8). Following transfection with miR-613 mimic, the expression of miR-613 was elevated in the BC cells leading to inhibition of NAMPT expression at both mRNA and protein level as measured by real-time PCR and western blotting. Our result identified a significant tumor suppressor role of miR-26b on NAMPT, NAD concentration, and cell viability in BC. Overall, based on our finding, miR-26b mimic transfection could elevate miR-26b levels in BC cells via downregulating the NAMPT expression, NAD expression levels, and cell growth, whereas miR-26b inhibitor had the opposite function. In conclusion, miR-26b can become a promising target for BC treatment through targeting NAMPT and inhibiting the NAD production.","PeriodicalId":11248,"journal":{"name":"DNA and cell biology","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40485360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

JMJD3 Promotes Porphyromonas gingivalis Lipopolysaccharide-Induced Th17-Cell Differentiation by Modulating the STAT3-RORc Signaling Pathway. JMJD3通过调节STAT3-RORc信号通路促进牙龈卟啉单胞菌脂多糖诱导的th17细胞分化

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-08-01 DOI: 10.1089/dna.2022.0149

Doudou Huang, Chi Zhang, Panpan Wang, Xiting Li, Li Gao, Chuanjiang Zhao

{"title":"JMJD3 Promotes Porphyromonas gingivalis Lipopolysaccharide-Induced Th17-Cell Differentiation by Modulating the STAT3-RORc Signaling Pathway.","authors":"Doudou Huang, Chi Zhang, Panpan Wang, Xiting Li, Li Gao, Chuanjiang Zhao","doi":"10.1089/dna.2022.0149","DOIUrl":"https://doi.org/10.1089/dna.2022.0149","url":null,"abstract":"The immune response mediated by Th17 cells is essential in the pathogenesis of periodontitis. Emerging evidence has demonstrated that lipopolysaccharide from Porphyromonas gingivalis (Pg-LPS) could promote Th17-cell differentiation directly, while the downstream signaling remains elusive. This study was aimed to explore the role of JMJD3 (a JmjC family histone demethylase) and signal transducers and activators of transcription 3 (STAT3) in Th17-cell differentiation triggered by Pg-LPS and clarify the interaction between them. We found that the expression of JMJD3 and STAT3 was significantly increased under Th17-polarizing conditions. Pg-LPS could promote Th17-cell differentiation from CD4+ T cells, with an increased expression of JMJD3 and STAT3 compared to the culture without Pg-LPS. The coimmunoprecipitation results showed that the interactions of JMJD3 and STAT3, STAT3 and retinoid-related orphan nuclear receptor γt (RORγt) were enhanced following Pg-LPS stimulation during Th17-cell differentiation. Further blocking assays were performed and the results showed that inhibition of STAT3 or JMJD3 both suppressed the Th17-cell differentiation, JMJD3 inhibitor could reduce the expression of STAT3 and p-STAT3, while JMJD3 expression was not affected when STAT3 was inhibited. Taken together, this study found that JMJD3 could promote Pg-LPS induced Th17-cell differentiation by modulating the STAT3-RORc signaling pathway.","PeriodicalId":11248,"journal":{"name":"DNA and cell biology","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9416562/pdf/dna.2022.0149.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10272110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Association Between Circulating Cell-Free DNA Level at Admission and the Risk of Heart Failure Incidence in Acute Myocardial Infarction Patients. 入院时循环游离细胞DNA水平与急性心肌梗死患者心力衰竭发生率的关系

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-08-01 Epub Date: 2022-06-28 DOI: 10.1089/dna.2022.0238

Qinghai Zhang, Xin He, Jing Ling, Qizhong Xiang, Minqi Li, Huiqi Zhao, Qinghua Fu, Yi Tang, Jin He, Wenjuan Fan, Yan Zhang, Hongwei Pan, Jianqiang Peng, Zhaofen Zheng

{"title":"Association Between Circulating Cell-Free DNA Level at Admission and the Risk of Heart Failure Incidence in Acute Myocardial Infarction Patients.","authors":"Qinghai Zhang, Xin He, Jing Ling, Qizhong Xiang, Minqi Li, Huiqi Zhao, Qinghua Fu, Yi Tang, Jin He, Wenjuan Fan, Yan Zhang, Hongwei Pan, Jianqiang Peng, Zhaofen Zheng","doi":"10.1089/dna.2022.0238","DOIUrl":"https://doi.org/10.1089/dna.2022.0238","url":null,"abstract":"Plasma cell-free DNA (cfDNA) was elevated in patients with acute myocardial infarction (AMI) or heart failure (HF). However, whether cfDNA could serve as a predictor for risk of HF after AMI remains unknown. In this study, we conducted a pilot prospective cohort study in which 98 AMI patients were enrolled from a single center to assess the association between cfDNA levels at admission and risk of HF in an AMI population. Patients with cfDNA above the median level (14.39 ng/mL) showed higher low-density lipoprotein cholesterol, cardiac troponin I (cTnI), and soluble suppression of tumorigenicity 2 (sST2) levels compared with patients below the median. cfDNA was positively correlated with cTnI (r = 0.377, p < 0.001) and sST2 (r = 0.443, p < 0.001). Within a median follow-up of about 345 days, 46 patients (52.6%) developed HF. Multivariate Cox analysis showed that a higher cfDNA (above the cutoff value: 9.227 ng/mL) was an effective risk predictor (C-index = 0.74, 95% confidence interval [CI]: 0.733-0.748) for HF incidence after AMI (adjusted hazard ratio [HR]: 2.805; 95% CI: 1.087-7.242; p = 0.033). Moreover, a linear association was observed between cfDNA and risk of HF incidence adjusted for by age, gender, and history of chronic kidney disease (p for linear trend = 0.044). Taken together, the cfDNA levels at admission are associated with the incidence of HF in AMI patients. A positive correlation between cfDNA and the fibrotic factor sST2 was proved, but the underlying mechanisms require further study.","PeriodicalId":11248,"journal":{"name":"DNA and cell biology","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40407134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2