DNA and cell biology最新文献_第9页

UFMylation Is Activated in Atherosclerosis of ApoE Knockout Mice. ApoE基因敲除小鼠动脉粥样硬化激活ufmyation。

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-10-01 Epub Date: 2022-09-07 DOI: 10.1089/dna.2022.0253

Yi Sun, Wendi Li, Zhenju Cao, Jiajia Hu, Mei Jia, Ming Su

{"title":"UFMylation Is Activated in Atherosclerosis of ApoE Knockout Mice.","authors":"Yi Sun, Wendi Li, Zhenju Cao, Jiajia Hu, Mei Jia, Ming Su","doi":"10.1089/dna.2022.0253","DOIUrl":"https://doi.org/10.1089/dna.2022.0253","url":null,"abstract":"UFMylation is a novel ubiquitin-like system that deals with complex and fine-tuned cellular activities and is closely related to endoplasmic reticulum stress. Our previous study indicated that UFMylation is activated in vascular remodeling models. However, the role of UFMylation in atherosclerosis (AS) is unclear. In this study, we investigated changes in UFMylation in ApoE knockout (ApoE-KO) mice. We found that UFMylation was significantly activated in ApoE-KO mice fed a high-fat diet for 46 weeks. Consistently we observed that vascular smooth muscle cells (VSMCs) treated with oxidized low-density lipoprotein (oxLDL) showed UFMylation activation in a time-dependent manner. UFM1-overexpressing mice were generated using transgenic (Tg) technique and bred with ApoE-KO mice to generate ApoE-KO/UFM1-Tg mice. We found that the degree of AS did not vary compared with that of the control. Similarly, overexpression of active UFM1 failed to alter oxLDL-induced proliferation of VSMCs. These findings indicate that UFMylation is activated in AS, but overexpression of UFM1 does not alter the development of AS in ApoE-KO mice.","PeriodicalId":11248,"journal":{"name":"DNA and cell biology","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40355727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Generating Human Induced Pluripotent Stem Cell Via Low-Dose Polyethylenimine-Mediated Transfection: An Optimized Protocol. 通过低剂量聚乙烯亚胺介导的转染产生人诱导多能干细胞:一个优化的方案。

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-10-01 Epub Date: 2022-08-18 DOI: 10.1089/dna.2022.0331

Monir Shayestehfar, Sara Farahi, Behjat Kheiri Yeganeh Azar, Amirhossein Memari, Tourandokht Baluchnejadmojarad, Faezeh Faghihi

{"title":"Generating Human Induced Pluripotent Stem Cell Via Low-Dose Polyethylenimine-Mediated Transfection: An Optimized Protocol.","authors":"Monir Shayestehfar, Sara Farahi, Behjat Kheiri Yeganeh Azar, Amirhossein Memari, Tourandokht Baluchnejadmojarad, Faezeh Faghihi","doi":"10.1089/dna.2022.0331","DOIUrl":"https://doi.org/10.1089/dna.2022.0331","url":null,"abstract":"Human dermal fibroblasts (HDFs) can be reprogrammed through different strategies to generate human induced pluripotent stem cells (hiPSCs). However, most of these strategies require high-cost materials and specific equipment not readily accessible in most laboratories. Hence, liposomal and virus-based techniques can replace with polyethylenimine (PEI)-mediated transfection to overcome these challenges. However, few researchers have addressed the PEI's ability to transfect HDFs. This study used PEI reagent to transfer oriP/EBNA1-based vector into HDFs to produce hiPSC lines. We first described conditions allowing the efficient transfection of HDFs with low cytotoxicity and without specific types of equipment and optimized several parameters relevant to the transfection procedure. We then monitored the effect of different N/P ratios on transfection efficiency and cytotoxicity using flow cytometry and fluorescent microscopy. By the results, we found that transfection efficiency was greatly affected by plasmid DNA concentration, PEI concentration, order of combining reagents, serum presence in polyplexes, and the duration of serum starvations. Moreover, using the optimized condition, we found that the N/P ratio of 3 achieved the highest percentage of HDFs positive for green fluorescent protein plasmid (∼40%) with minimal cell toxicity. We finally generated hiPSCs using the optimized protocol and oriP/EBNA1-based vectors. We confirmed hiPSC formation by characterizing tests: alkaline phosphatase staining, immunocytochemistry assay, real-time PCR analysis, in vitro differentiation into three germ layers, and karyotyping test. In conclusion, our results indicated that 25 kDa branched PEI could efficiently transfect HDFs toward generating hiPSCs via a simple, cost-effective, and optimized condition.","PeriodicalId":11248,"journal":{"name":"DNA and cell biology","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40623319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Mitophagy: Critical Role in Atherosclerosis Progression. 线粒体自噬:动脉粥样硬化进展中的关键作用。

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-10-01 Epub Date: 2022-08-26 DOI: 10.1089/dna.2022.0249

Yanmei Chen, Wenhua Qin, Lu Li, Peng Wu, Dangheng Wei

引用次数: 3

Is Parthanatos Involved in Varicocele? Parthanatos与精索静脉曲张有关吗?

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-10-01 Epub Date: 2022-09-02 DOI: 10.1089/dna.2022.0289

Kanxian Wang, Yuanyuan Gao, Chen Wang, Zirui Liu, Meng Liang, Yaping Liao, Ke Hu

{"title":"Is Parthanatos Involved in Varicocele?","authors":"Kanxian Wang, Yuanyuan Gao, Chen Wang, Zirui Liu, Meng Liang, Yaping Liao, Ke Hu","doi":"10.1089/dna.2022.0289","DOIUrl":"https://doi.org/10.1089/dna.2022.0289","url":null,"abstract":"Varicoceles (VCs) have received widespread attention as a primary factor affecting male fertility and a pathological condition that may lead to decreased sperm count and motility in patients. Many studies have shown that an imbalance of local antioxidant balance exists in patients with VC, leading to an obvious increase in the content of reactive oxygen species (ROS) and may cause reductive stress. Excessive ROS may aggravate spermatogenesis dysfunction and affect male fertility. Poly(ADP-ribose) polymerase (PARP) is an enzyme associated with DNA repair in eukaryotic cells, can be activated by DNA fragments with structural damage, and has been considered a DNA damage receptor in DNA damage repair and apoptosis. We built a rat model of VC and an oxidative damage model of a spermatocyte-derived cell line (GC-2 cells) induced by hydrogen peroxide to study the role of PARP1 in VC. Differentially expressed genes (DEGs) were obtained by RNA sequencing in the testes of VC rats. Analysis of DEGs revealed some genes with significantly altered expression, which were validated in rat and cell models. Immunofluorescence, real-time quantitative PCR analysis, Western blot, and flow cytometry were used to analyze the changes between the control group and the VC or hydrogen peroxide group. Overall, we found that PARP1 protein expression increased in VC rats and in the hydrogen peroxide-induced oxidative stress model of GC-2 cells. Parthanatos may be one of the factors leading to reduced reproductive capacity in VC patients. Our study provides novel insights into the mechanisms of male infertility induced by oxidative stress and provides a new therapeutic target for VC.","PeriodicalId":11248,"journal":{"name":"DNA and cell biology","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40353288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

SPAG5 Activates PI3K/AKT Pathway and Promotes the Tumor Progression and Chemo-Resistance in Gastric Cancer. SPAG5激活PI3K/AKT通路，促进癌症的肿瘤进展和化疗耐药性。

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-10-01 DOI: 10.1089/dna.2021.0531

Juan An, Lang Yang, Yuanming Pan, Yuqi He, Hui Xie, Yurong Tao, Wei Li, Yupeng Yan, Siai Chen, Ya Liu, Xiaoming Ma, Ling An, Dongde Ji, Zhanhai Su, Jianqiu Sheng

{"title":"SPAG5 Activates PI3K/AKT Pathway and Promotes the Tumor Progression and Chemo-Resistance in Gastric Cancer.","authors":"Juan An, Lang Yang, Yuanming Pan, Yuqi He, Hui Xie, Yurong Tao, Wei Li, Yupeng Yan, Siai Chen, Ya Liu, Xiaoming Ma, Ling An, Dongde Ji, Zhanhai Su, Jianqiu Sheng","doi":"10.1089/dna.2021.0531","DOIUrl":"10.1089/dna.2021.0531","url":null,"abstract":"The sperm-associated antigen 5 (SPAG5) is an important protein in mitosis and cell cycle checkpoint regulation, with more attention as a novel oncogene in various cancers. High level of SPAG5 expression has been detected in our clinical gastric cancer (GC) samples and The Cancer Genome Atlas GC data. However, the bio-function and potential mechanism of SPAG5 in GC remain unclear. In this study, we investigated the role of SPAG5 in GC development and the correlation between SPAG5 and 5-fluorouracil (5-FU) treatment. SPAG5 expression was increased in GC samples compared with that in normal tissues (80.8% vs. 22.0%), which was apparently associated with a worse outcome. Biological experiments showed that knockdown of SPAG5 induced apoptosis and suppressed proliferation in cells and animal models. Downregulation of SPAG5 enhanced the sensitivity of 5-FU in GC cells. Gene microarray chip identified 856 upregulated and 787 downregulated genes in SPAG5 silencing cells. Furthermore, 12 significant genes, including CDKN1A, CDKN1B, EIF4E, MAPK1, and HSP90B1, belonged to the PI3K/AKT signaling pathway using ingenuity pathway analysis. Meanwhile, real-time PCR and Western blotting results showed that knockdown of SPAG5 inhibited PI3K/AKT signaling pathway. Collectively, SPAG5 promotes the growth of GC cells by regulating PI3K/AKT signaling pathway, which could be the promising target gene in GC therapy.","PeriodicalId":11248,"journal":{"name":"DNA and cell biology","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40669672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Genome-Wide Scan Reveals Toll-Like Receptor Contraction Events in Oplegnathidae. 全基因组扫描揭示了蚁科toll样受体收缩事件。

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-10-01 Epub Date: 2022-09-14 DOI: 10.1089/dna.2022.0234

Yanduo Wu, Yongshuang Xiao, Zhizhong Xiao, Yuting Ma, Haixia Zhao, Guang Gao, Jun Li

{"title":"Genome-Wide Scan Reveals Toll-Like Receptor Contraction Events in Oplegnathidae.","authors":"Yanduo Wu, Yongshuang Xiao, Zhizhong Xiao, Yuting Ma, Haixia Zhao, Guang Gao, Jun Li","doi":"10.1089/dna.2022.0234","DOIUrl":"https://doi.org/10.1089/dna.2022.0234","url":null,"abstract":"The striped knifejaw (Oplegnathus fasciatus) and spotted knifejaw (Oplegnathus punctatus) are prominent members of the Oplegnathidae family and are rocky reef-loving fishes with high ecological and economic value. However, the frequent occurrence of diseases in these fishes has severely restricted the development of their breeding industry. Toll-like receptors (TLRs) play an important role in resistance to pathogens as part of innate immunity. Genome-wide scans and cross-species comparative analysis revealed 10 TLRs in O. fasciatus (OfTLRs) and only 5 in O. punctatus (OpTLRs). In contrast to those of mammals and other fishes, the TLR family of Oplegnathidae underwent significant contraction events, especially in O. punctatus (only TLR1, TLR2, TLR14, TLR5, and TLR21 were retained). A phylogenetic tree divided the 10 OfTLRs into 5 subfamilies: TLR1, TLR3, TLR5, TLR7, and TLR11. The five OpTLR genes were divided into three different subfamilies: TLR1, TLR5, and TLR11. Quantitative real-time PCR revealed that all OpTLRs were expressed in the examined tissues, especially the immune system-related tissues, such as the spleen, gill, head kidney, and middle kidney. The expression of OpTLRs was high at the early stage of development (5 days posthatching [dph]) and decreased gradually until 30 dph. We speculated that maternal immunity or the developmental function of TLRs played an important protective role in the early stage. However, from 30 to 60 dph, TLR expression was low. At this time, juvenile fish are susceptible to viruses and begin to show TLR self-expression with weak immunity. Artificial immunity enhancement is needed to improve the environmental resistance of juvenile fish. In summary, our results not only provide valuable basic data for future studies of the TLR gene family in Oplegnathidae fish but also lay a solid foundation for Oplegnathidae fish research.","PeriodicalId":11248,"journal":{"name":"DNA and cell biology","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40359151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Correction to: MicroRNA-26b Reduces Cell Viability by Inhibition of Nicotinamide Phosphoribosyltransferase in Breast Cancer Cells, by Melika Ameli Mojarad et al. DNA Cell Biol. 2022;41(8):735-741; doi: 10.1089/dna.2022.0214. 更正:MicroRNA-26b通过抑制乳腺癌细胞中烟酰胺磷酸核糖基转移酶降低细胞活力，由Melika Ameli Mojarad等人。中国生物医学工程学报，2011;41(8):735-741;doi: 10.1089 / dna.2022.0214。

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-10-01 Epub Date: 2022-09-29 DOI: 10.1089/dna.2022.0214.correx

引用次数: 0

A Brief Update for Our Readers. 给读者的简短更新。

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-09-01 Epub Date: 2022-07-12 DOI: 10.1089/dna.2022.29021.editorial

Carol Shoshkes Reiss

引用次数: 0

The Role of Alternative Splicing in Cancer: Regulatory Mechanism, Therapeutic Strategy, and Bioinformatics Application. 选择性剪接在癌症中的作用:调控机制、治疗策略和生物信息学应用。

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-09-01 Epub Date: 2022-08-10 DOI: 10.1089/dna.2022.0322

Yao-Jie Pan, Bo-Wen Liu, Dong-Sheng Pei

引用次数: 0

The Regulation of Autophagy by p38 MAPK-PPARγ Signaling During the Brain Ischemic Tolerance Induced by Cerebral Ischemic Preconditioning. 脑缺血预处理诱导脑缺血耐受过程中p38 MAPK-PPARγ信号对自噬的调控

IF 3.1 4区生物学

DNA and cell biology Pub Date : 2022-09-01 Epub Date: 2022-08-09 DOI: 10.1089/dna.2022.0087

A-Chou Su, Ling-Yan Zhang, Jing-Ge Zhang, Yu-Yan Hu, Xi-Yun Liu, Shi-Chao Li, Xiao-Hui Xian, Wen-Bin Li, Min Zhang

{"title":"The Regulation of Autophagy by p38 MAPK-PPARγ Signaling During the Brain Ischemic Tolerance Induced by Cerebral Ischemic Preconditioning.","authors":"A-Chou Su, Ling-Yan Zhang, Jing-Ge Zhang, Yu-Yan Hu, Xi-Yun Liu, Shi-Chao Li, Xiao-Hui Xian, Wen-Bin Li, Min Zhang","doi":"10.1089/dna.2022.0087","DOIUrl":"https://doi.org/10.1089/dna.2022.0087","url":null,"abstract":"Several studies indicated that autophagy activation participates in brain ischemic tolerance (BIT) induced by cerebral ischemic preconditioning (CIP). However, the mechanism of autophagy activation during the process still remains unclear. The present study aimed to evaluate the role of p38 MAPK-peroxisome proliferator-activated receptor γ (PPARγ) signaling cascade in autophagy during the CIP-induced BIT. The results shown that, initially, autophagy activation was observed after CIP in the model of global cerebral ischemia in rats, as was indicated by the upregulation of Beclin 1 expression, an increase in LC3-II/LC3-I ratio, the enhanced LC3 immunofluorescence, and a rise in the number of autophagosomes in the neurons of the hippocampal CA1 area. Besides, the inhibitor of autophagy 3-methyladenine obliterated the neuroprotection induced by CIP. Furthermore, the upregulation of p-p38 MAPK and PPARγ expressions was earlier than autophagy activation after CIP. In addition, pretreatment with SB203580 (the inhibitor of p38 MAPK) reversed CIP-induced PPARγ upregulation, autophagy activation, and neuroprotection. Pretreatment with GW9662 (the inhibitor of PPARγ) reversed autophagy activation and neuroprotection, while it had no effect on p-p38 MAPK upregulation induced by CIP. These data suggested that the p38 MAPK-PPARγ signaling pathway participates in autophagy activation during the induction of BIT by CIP.","PeriodicalId":11248,"journal":{"name":"DNA and cell biology","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40613553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3