Quantitative Data-Independent Acquisition Mass Spectrometry Proteomics and Weighted Correlation Network Analysis of Plasma Samples for the Discovery of Chronic Kidney Disease-Specific Atherosclerosis Risk Factors.

IF 2.6 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
DNA and cell biology Pub Date : 2022-11-01 Epub Date: 2022-10-17 DOI:10.1089/dna.2022.0200
Daopeng Dai, Zhiwei Cheng, Shuo Feng, Zhengbin Zhu, Jiwei Yu, Wenli Zhang, Hui Lu, Ruiyan Zhang, Jinzhou Zhu
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引用次数: 0

Abstract

Chronic kidney disease (CKD) accelerates atherosclerosis. The mechanism of CKD-related atherosclerosis is complex, and CKD-specific risk factors may contribute to this process in addition to traditional risk factors such as hypertension, diabetes, and hypercholesterolemia. In the present study, to discover CKD-specific atherosclerosis risk factors, a total of 62 patients with different stages of kidney function were enrolled. All patients underwent coronary angiographies and the severity of coronary atherosclerosis was defined by the SYNTAX score. Patients were divided into different groups according to their kidney function levels and coronary atherosclerosis severity. Data-independent acquisition mass spectrometry was used to identify differentially expressed proteins (DEPs) in the plasma samples, and weighted correlation network analysis (WGCNA) was employed to identify significant protein modules and hub proteins related to CKD-specific atherosclerosis. The results showed that 10 DEPs associated with atherosclerosis were found in the comparative groups with modest and severe CKD. Through WGCNA, 1768 proteins were identified and 8 protein modules were established. Enrichment analyses of protein modules revealed functional clusters mainly associated with inflammation and the complement and coagulation cascade as atherosclerosis developed under CKD conditions. The results may help to better understand the mechanisms of CKD-related atherosclerosis and guide future research on developing treatments for CKD-related atherosclerosis.

血浆样本的定量数据独立获取质谱蛋白质组学和加权相关网络分析用于发现慢性肾脏疾病特异性动脉粥样硬化危险因素。
慢性肾脏疾病(CKD)加速动脉粥样硬化。ckd相关动脉粥样硬化的机制是复杂的,除了传统的危险因素如高血压、糖尿病、高胆固醇血症外,ckd特异性的危险因素也可能参与这一过程。在本研究中,为了发现ckd特异性动脉粥样硬化的危险因素,共入组62例不同阶段肾功能的患者。所有患者均接受冠状动脉造影,冠状动脉粥样硬化的严重程度由SYNTAX评分确定。根据患者的肾功能水平和冠状动脉粥样硬化严重程度将患者分为不同的组。采用数据独立采集质谱法鉴定血浆样品中的差异表达蛋白(DEPs),并采用加权相关网络分析(WGCNA)鉴定与ckd特异性动脉粥样硬化相关的重要蛋白模块和枢纽蛋白。结果显示,在中度和重度CKD对照组中发现了10个与动脉粥样硬化相关的dep。通过WGCNA鉴定了1768个蛋白,建立了8个蛋白模块。蛋白质模块的富集分析显示,CKD条件下动脉粥样硬化发生时,功能簇主要与炎症、补体和凝血级联相关。这些结果可能有助于更好地了解ckd相关动脉粥样硬化的机制,并指导未来ckd相关动脉粥样硬化的治疗研究。
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来源期刊
DNA and cell biology
DNA and cell biology 生物-生化与分子生物学
CiteScore
6.60
自引率
0.00%
发文量
93
审稿时长
1.5 months
期刊介绍: DNA and Cell Biology delivers authoritative, peer-reviewed research on all aspects of molecular and cellular biology, with a unique focus on combining mechanistic and clinical studies to drive the field forward. DNA and Cell Biology coverage includes: Gene Structure, Function, and Regulation Gene regulation Molecular mechanisms of cell activation Mechanisms of transcriptional, translational, or epigenetic control of gene expression Molecular Medicine Molecular pathogenesis Genetic approaches to cancer and autoimmune diseases Translational studies in cell and molecular biology Cellular Organelles Autophagy Apoptosis P bodies Peroxisosomes Protein Biosynthesis and Degradation Regulation of protein synthesis Post-translational modifications Control of degradation Cell-Autonomous Inflammation and Host Cell Response to Infection Responses to cytokines and other physiological mediators Evasive pathways of pathogens.
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