Developmental biology最新文献_第8页

TMBIM4 affects left-right patterning via pluripotency exit during gastrulation TMBIM4在原肠胚形成过程中通过多能性退出影响左右模式。

IF 2.1 3区生物学

Developmental biology Pub Date : 2025-07-29 DOI: 10.1016/j.ydbio.2025.07.018

Nicholas S. Diab , Valentyna Kostiuk , Leonid Tyan , Emily Mis , David Zenisek , Mustafa K. Khokha

{"title":"TMBIM4 affects left-right patterning via pluripotency exit during gastrulation","authors":"Nicholas S. Diab , Valentyna Kostiuk , Leonid Tyan , Emily Mis , David Zenisek , Mustafa K. Khokha","doi":"10.1016/j.ydbio.2025.07.018","DOIUrl":"10.1016/j.ydbio.2025.07.018","url":null,"abstract":"<div><div>Congenital heart disease (CHD) is the most prevalent congenital defect, but its underlying genetic and developmental mechanisms remain incompletely understood. Transmembrane BAX inhibitor motif-containing protein 4 (<em>TMBIM4</em>) has emerged as a candidate gene from genomic studies in CHD patients. Patients with deleterious genetic variation in <em>TMBIM4</em> can exhibit cardiac heterotaxy, a type of left-right (LR) patterning defect characterized by abnormal cardiac asymmetry. Using <em>Xenopus tropicalis</em>, we investigated <em>tmbim4's</em> developmental roles and identified its critical function in LR patterning. <em>tmbim4</em> depletion in <em>Xenopus</em> produced cardiac asymmetry defects which could be rescued by human and viral orthologs of the protein, reflecting remarkable evolutionary conservation. We identified gastrulation as a critical window for <em>tmbim4</em> function. <em>tmbim4</em> depletion impairs gastrulation, leading to abnormal pluripotency marker expression and delayed pluripotency exit. TMBIM4's underlying function is as a putative ion channel, and ion channels are emerging as key regulators of LR patterning and cell fate determination. Using sharp electrodes to measure membrane potential (V<sub>m</sub>), <em>tmbim4</em> depletion depolarized affected embryos. The application of choline, which we have previously shown recues depolarization of <em>Xenopus</em> embryos, rescued the gastrulation defects and pluripotency in <em>tmbim4</em> depleted embryos. Interestingly, TMBIM4 has previously been localized to the Golgi, and therefore how it might affect V<sub>m</sub> was unclear. We find evidence that TMBIM4 localizes to the plasma membrane as well as the Golgi suggesting that it may directly act to establish cellular V<sub>m</sub>. Our results establish <em>tmbim4</em> as a plausible CHD gene and offer the first study of <em>tmbim4</em> in a developmental context.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"527 ","pages":"Pages 136-146"},"PeriodicalIF":2.1,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144759391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Germline factors, TDRD and Piwi, colocalize with Vasa on the mitotic apparatus during the embryogenesis of the sea urchin 在海胆胚胎发生过程中，种系因子TDRD和PIWI与Vasa共定位于有丝分裂器上。

IF 2.1 3区生物学

Developmental biology Pub Date : 2025-07-29 DOI: 10.1016/j.ydbio.2025.07.016

Mariana Witmer , Nirali Mehta , Natsuko Emura , Mamiko Yajima

{"title":"Germline factors, TDRD and Piwi, colocalize with Vasa on the mitotic apparatus during the embryogenesis of the sea urchin","authors":"Mariana Witmer , Nirali Mehta , Natsuko Emura , Mamiko Yajima","doi":"10.1016/j.ydbio.2025.07.016","DOIUrl":"10.1016/j.ydbio.2025.07.016","url":null,"abstract":"<div><div>Germline factors are thought to function exclusively in the germline, providing the unique characteristics of germ cells. However, recent studies suggest that some of these factors may also be expressed and function outside the germline. One such example includes Vasa, a DEAD-box RNA helicase that appears to control localized translation on the spindle, facilitating efficient protein synthesis during embryogenesis of the sea urchin. However, it remains unclear if other germline factors are also involved in this process. In this study, we investigated the localization dynamics of Vasa's partners in the germline, such as Tudor-domain-containing proteins (TDRDs) and P-element-induced wimpy testis proteins (Piwis). Among TDRDs tested in this study, we found that TDRD7 is enriched on the spindle and forms granules with Vasa during early embryogenesis. Vasa and TDRD7 recruited each other when the expression of either was forced at the membrane, suggesting their interaction with each other. TDRD7 mutants lacking the N-terminal eLOTUS domain or the central intrinsically disordered region exhibited reduced granule formation, which also compromised their recruitment to Vasa. In contrast, PiwiL1/2 and PiwiL3 showed enrichment at the perinuclear region and the spindle, yet were never recruited to Vasa or TDRD7 when either was expressed at the membrane. These results suggest that a group of germline factors is present and may dynamically interact with each other on the spindle, contributing to somatic cell regulation in the sea urchin embryo.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"527 ","pages":"Pages 98-108"},"PeriodicalIF":2.1,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144759390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ShH10 viral vector for safe, efficient, and selective transduction of inner ear supporting cells 安全、高效、选择性转导内耳支持细胞的ShH10病毒载体。

IF 2.1 3区生物学

Developmental biology Pub Date : 2025-07-28 DOI: 10.1016/j.ydbio.2025.07.013

Yun Ji Bertken , Yeeun Kim , Juan Llamas , Assaf Beck , Aaron Nagiel , Ksenia Gnedeva

引用次数: 0

A low-cost, high-throughput pipeline for 3D imaging of embryonic mouse hearts using lightsheet microscopy 一种低成本、高通量的胚胎小鼠心脏三维成像通道。

IF 2.1 3区生物学

Developmental biology Pub Date : 2025-07-26 DOI: 10.1016/j.ydbio.2025.07.009

Xiangyang Liu , Jianfeng Wang , Youshi Chen , Hongjun Shi

{"title":"A low-cost, high-throughput pipeline for 3D imaging of embryonic mouse hearts using lightsheet microscopy","authors":"Xiangyang Liu , Jianfeng Wang , Youshi Chen , Hongjun Shi","doi":"10.1016/j.ydbio.2025.07.009","DOIUrl":"10.1016/j.ydbio.2025.07.009","url":null,"abstract":"<div><div>Lightsheet microscopy is a powerful tool for three-dimensional imaging of both live and fixed specimens, spanning small to large scales. However, the time-intensive sample preparation and mounting process often limit its use in high-throughput studies. Given our focus on cardiac development and identifying cardiovascular abnormalities after teratogenic exposure, we have developed an efficient, low-cost, and user-friendly system for specimen fixation, clearing, and mounting. This system enables rapid 3D imaging of a mouse embryonic heart within 1 min using the Zeiss Lightsheet Z.1 microscopy and supports imaging of at least 30 hearts per hour with high resolution. With this system, we obtained high-quality images of embryonic hearts at various stages, visualizing internal structures like the aortic valve and coronary arteries with this system. We further demonstrated its capability for quantitative analysis, including endocardial cushion cell density at E10.5 and volumetric measurements of valve morphology. As an extended application, the system was also applied to postnatal P10 hearts and extra-cardiac organs like kidney and ovary, showing clear structural detail. Additionally, integration of the water-soluble clearing agent, EZ Clear, alongside Cre-loxP-mediated genetic lineage tracing, enabled 3D visualization of cellular contributions to heart development with high resolution. The sample preparation system described here promises broader applications in embryology, anatomy, and pathology research, especially in studies requiring both high throughput and high resolution of 3D imaging.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"527 ","pages":"Pages 26-38"},"PeriodicalIF":2.1,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144728526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PRDM13 is required for specification of PAX2 lineage inhibitory neurons in the developing cerebellum PRDM13是发育中的小脑中PAX2谱系抑制神经元的指定所必需的。

IF 2.1 3区生物学

Developmental biology Pub Date : 2025-07-26 DOI: 10.1016/j.ydbio.2025.07.012

Z. Zack Ma , Michael A. Hale , Bishakha Mona , Ana Uruena , Jane E. Johnson

{"title":"PRDM13 is required for specification of PAX2 lineage inhibitory neurons in the developing cerebellum","authors":"Z. Zack Ma , Michael A. Hale , Bishakha Mona , Ana Uruena , Jane E. Johnson","doi":"10.1016/j.ydbio.2025.07.012","DOIUrl":"10.1016/j.ydbio.2025.07.012","url":null,"abstract":"<div><div>Shared genetic developmental programs in which specific transcription factors affect similar cell fate decisions in distinct tissues are common. In the developing dorsal neural tube and cerebellum, PTF1A is essential for specification of GABAergic inhibitory neurons and suppression of alternative glutamatergic excitatory neuronal fates. Previous studies in the mouse dorsal neural tube identified the transcriptional repressor PRDM13 as a transcriptional target of PTF1A that functions to suppress the alternate cell fates to ensure precision in neuronal cell identity. The presence of PRDM13 in PTF1A + cerebellar progenitors suggests a similar role for PRDM13 in cerebellar neuronal specification. Cerebellar agenesis in humans with missense mutations in <em>PRDM13</em>, and perturbations in cerebellar development in <em>Prdm13</em> mutant mice and zebrafish, confirm PRDM13 requirement in this tissue. Here we add to these findings showing additional mutant alleles in mouse <em>Prdm13</em> phenocopy the perturbation in cerebellar cell fates seen with the absence of PTF1A, including loss of PAX2+ interneuron and Purkinje cell inhibitory neuronal lineages, increases in TLX3+ excitatory neuronal lineages, increased apoptosis, and reduced cerebellar size. Additional defects are seen in the placement of TBR1+ cerebellar cells. Thus, using <em>Prdm13</em> mutant mice, we support conclusions that PRDM13 functions to specify balanced numbers of inhibitory and excitatory neuronal progenitors in the developing cerebellum.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"527 ","pages":"Pages 17-25"},"PeriodicalIF":2.1,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144728527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potentials of RNA biosensors in developmental biology RNA生物传感器在发育生物学中的应用前景

IF 2.1 3区生物学

Developmental biology Pub Date : 2025-07-26 DOI: 10.1016/j.ydbio.2025.07.011

Ehsan Pashay Ahi , Mehran Khorshid

{"title":"Potentials of RNA biosensors in developmental biology","authors":"Ehsan Pashay Ahi , Mehran Khorshid","doi":"10.1016/j.ydbio.2025.07.011","DOIUrl":"10.1016/j.ydbio.2025.07.011","url":null,"abstract":"<div><div>RNA-based/associated biosensors represent a rapidly expanding area of research, providing highly sensitive tools for detecting and monitoring RNA in diverse biological contexts. These sensors offer the ability to track RNA localization, modifications, and interactions in real-time, making them particularly well-suited for developmental biology research. Despite their demonstrated utility in fields such as diagnostics, synthetic biology and environmental science, the application of RNA biosensors in developmental biology has only begun to emerge within the past decade. This gap is notable given the potential of these tools to address key questions about spatiotemporal RNA regulation and cellular signaling during development. This perspective review presents a selection of RNA biosensors, including fluorescent RNA aptamers, CRISPR-Cas-based systems, riboswitches, and catalytic RNA sensors, which have gained attraction in other scientific disciplines. These tools can be used not only to study intrinsic RNA biology, such as RNA expression, splicing, and localization, but also to detect the effects of extrinsic physical and chemical factors, including pH, temperature, redox state, and mechanical stress, on RNA behavior during developmental processes. These examples illustrate how RNA biosensors could be adapted to study developmental mechanisms in model organisms, enabling investigations into RNA dynamics and their role in shaping developmental processes. By revisiting these underutilized tools, this review highlights their relevance for advancing the understanding of molecular mechanisms in developmental biology studies.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"526 ","pages":"Pages 173-188"},"PeriodicalIF":2.1,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144722135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteins involved in cell division have broad developmental functions 参与细胞分裂的蛋白质具有广泛的发育功能。

IF 2.1 3区生物学

Developmental biology Pub Date : 2025-07-25 DOI: 10.1016/j.ydbio.2025.07.008

Jessica Benito , Elizabeth McCulla , Raisa Sumaiya, Jia L. Song

{"title":"Proteins involved in cell division have broad developmental functions","authors":"Jessica Benito , Elizabeth McCulla , Raisa Sumaiya, Jia L. Song","doi":"10.1016/j.ydbio.2025.07.008","DOIUrl":"10.1016/j.ydbio.2025.07.008","url":null,"abstract":"<div><div>Several proteins that are known to play a crucial role in mitosis may have alternative functions in embryogenesis. To test this hypothesis, we examined the spatial and temporal expression of the transcripts that encode proteins involved in mitosis throughout development, including those that encode for motor proteins, cytoskeletal elements and their modulators, vesicular transport, and cell cycle regulators. Results indicate that these transcripts have different expression patterns in various cell types. Interestingly, <em>Cyclin Dependent Kinase 1</em> (<em>CDK1</em>), <em>Polo Like Kinase 1</em> (<em>PLK1</em>), and <em>Aurora kinase</em> (<em>Aurk</em>) transcripts are expressed by endomesodermal cells of the blastula, the multipotent stem cells in coelomic pouches and/or the skeletogenic mesoderm of the gastrula that are not actively dividing. To further test that proteins important for mitosis may perform additional functions during embryogenesis, we treated embryos with CDK1, PLK1, and Aurk inhibitors, which resulted in a dose-dependent developmental arrest or delay and defects in gastrulation, skeletogenesis, and epithelial to mesenchymal transition. Further analysis indicates that the number of mesodermally-derived pigment cells is significantly less in CDK1 and PLK1 inhibited embryos and significantly increased in Aurk inhibited embryos. Importantly, the percentage of pigment cells undergoing cell proliferation in drug-treated embryos was not different than the control, indicating additional functions of CDK1, PLK1, and Aurk. Furthermore, PLK1 and Aurk may regulate ERK signaling to impact various developmental processes.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"527 ","pages":"Pages 1-16"},"PeriodicalIF":2.1,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144728528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IPPK-1 and IP6 contribute to ventral nerve cord assembly in C. elegans IPPK-1和IP6参与秀丽隐杆线虫腹侧神经索组装。

IF 2.5 3区生物学

Developmental biology Pub Date : 2025-07-18 DOI: 10.1016/j.ydbio.2025.07.007

Nathaniel Noblett , Tony Roenspies , Chloe B. Kirezi , Clover Stubbert , Stephane Flibotte , Pavak K. Shah , Antonio Colavita

{"title":"IPPK-1 and IP6 contribute to ventral nerve cord assembly in C. elegans","authors":"Nathaniel Noblett , Tony Roenspies , Chloe B. Kirezi , Clover Stubbert , Stephane Flibotte , Pavak K. Shah , Antonio Colavita","doi":"10.1016/j.ydbio.2025.07.007","DOIUrl":"10.1016/j.ydbio.2025.07.007","url":null,"abstract":"<div><div>Inositol phosphates (IPs) are essential for the development and function of the nervous system. Loss-of-function studies, which demonstrate the importance of specific IP isomers, show their critical role in proper neural tube formation. In this study, we show that inositol pentakisphosphate 2-kinase (IPPK-1), the kinase that phosphorylates IP5 to generate IP6, is involved in assembling the ventral nerve cord (VNC) in <em>C. elegans</em>. We show that mutations in <em>ippk-1</em> lead to the mispositioning of motor neurons along the VNC of newly hatched larvae. These positioning defects reflect disruption of VNC assembly during embryogenesis, as VNC neuronal progenitors in <em>ippk-1</em> embryos display a more compact organization after arising on the left and right sides of the embryo, delays in rosette-mediated convergent extension, and defects in cell intercalation. We further show that injection of exogenous IP6 into the gonads of <em>ippk-1</em> mutants can rescue both embryonic and neuron positioning defects. Our findings indicate that IP isomers, particularly IP6, are important for ventral nerve cord formation in <em>C. elegans</em>. Along with their role in neural tube formation in vertebrates, these results suggests that IP isomers play an ancient role in central nerve cord development.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"526 ","pages":"Pages 159-172"},"PeriodicalIF":2.5,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144674069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PROX1 is necessary for cellular patterning, innervation and differentiation in the mouse organ of Corti PROX1在小鼠Corti器官的细胞模式、神经支配和分化中是必需的

IF 2.5 3区生物学

Developmental biology Pub Date : 2025-07-17 DOI: 10.1016/j.ydbio.2025.07.006

Shubham Kale , Dimitri Traenkner , Ellison J. Goodrich , Satish R. Ghimire , Thomas M. Coate , Michael R. Deans

{"title":"PROX1 is necessary for cellular patterning, innervation and differentiation in the mouse organ of Corti","authors":"Shubham Kale , Dimitri Traenkner , Ellison J. Goodrich , Satish R. Ghimire , Thomas M. Coate , Michael R. Deans","doi":"10.1016/j.ydbio.2025.07.006","DOIUrl":"10.1016/j.ydbio.2025.07.006","url":null,"abstract":"<div><div>The organ of Corti is divided into functional compartments responsible for hearing or cochlear amplification. A medial compartment containing inner hair cells innervated by Type I spiral ganglion neurons and a lateral compartment containing outer hair cells innervated by Type II spiral ganglion neurons. Supporting cells also differ, with lateral compartment pillar cells and Deiters' cells developing specialized cellular structures to support outer hair cell electromotility. We bred organ of Corti-restricted <em>Prox1</em> conditional knockout mice to study lateral compartment development because PROX1 is the first transcription factor expressed strictly in lateral compartment supporting cells. In the absence of <em>Prox1</em>, supporting cell numbers increased without corresponding changes in outer hair cells, and they appear incompletely differentiated based on morphological criteria. Outer hair cell number was not impacted but innervation was disrupted with many afferent neurons turning incorrectly towards the cochlear apex. RNAseq revealed no changes in gene expression that could account for the innervation phenotype. Therefore, we propose that PROX1 promotes pillar and Deiters’ cell differentiation and organization that has secondary effects on innervation but is not required for compartment specification.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"526 ","pages":"Pages 133-146"},"PeriodicalIF":2.5,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantifying convergent extension: Shih and Keller's quintessential work in developmental cell biology 量化趋同扩展：Shih和Keller在发育细胞生物学中的典型工作。

IF 2.5 3区生物学

Developmental biology Pub Date : 2025-07-15 DOI: 10.1016/j.ydbio.2025.07.005

Robert J. Huebner, John B. Wallingford

引用次数: 0