Developmental biology最新文献

PRMT3 Gene Expression and Methylation Levels in Arrested Embryos: Implications for Developmental Arrest Defects.

IF 2.5 3区生物学

Developmental biology Pub Date : 2025-01-30 DOI: 10.1016/j.ydbio.2025.01.018

Wuwen Zhang, Shifeng Li, Kai Li, Ningyu Sun, Rong Lv, Jie Ma, Ping Yin, Guoqing Tong, Yuanyuan Chen, Lu Lu, Yun Li, Yuanyuan Wu, Hua Yan

{"title":"PRMT3 Gene Expression and Methylation Levels in Arrested Embryos: Implications for Developmental Arrest Defects.","authors":"Wuwen Zhang, Shifeng Li, Kai Li, Ningyu Sun, Rong Lv, Jie Ma, Ping Yin, Guoqing Tong, Yuanyuan Chen, Lu Lu, Yun Li, Yuanyuan Wu, Hua Yan","doi":"10.1016/j.ydbio.2025.01.018","DOIUrl":"https://doi.org/10.1016/j.ydbio.2025.01.018","url":null,"abstract":"Embryos generated through in vitro fertilization (IVF) frequently experience developmental arrests or blocks, which significantly reduces the success rate of IVF therapy. Recent studies have shown that the protein arginine methylase 3 (PRMT3) plays a crucial role in the regulating of gene expression during early embryonic development. However, the exact regulatory mechanisms of PRMT3 involved in early embryonic development are still unclear. In this study, we used discarded arrested and polyspermic embryos from IVF for experiments, employing confocal techniques and qRT-PCR to examine PRMT3 expression and changes in H4R3me2a methylation during various stages of early development. Furthermore, PRMT3 was re-expressed in the arrested embryos to observe their subsequent development. Our findings revealed that PRMT3 nucleic acid and protein were significantly lower in arrested embryos than in control embryos (P<0.05). Additionally, methylation levels of H4R3me2a were significantly lower in arrested embryos (P<0.05). Re-expression of PRMT3 could partially rescue embryos that are developmentally arrested, and even a few arrested embryos have the potential to develop into morula or blastocysts. In summary, the reduction or deletion of PRMT3 gene in early embryo may lead to developmental arrested defects. Therefore, it is crucial to regulate the expression and functioning of PRMT3 for the proper development of early embryos, and further research is required to investigate potential therapeutic interventions for embryonic development arrest in vitro.","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluid secretion and luminal pressure control lateral branching morphogenesis in the embryonic avian lung.

IF 2.5 3区生物学

Developmental biology Pub Date : 2025-01-25 DOI: 10.1016/j.ydbio.2025.01.016

Shelby R Mohr-Allen, Jason P Gleghorn, Victor D Varner

{"title":"Fluid secretion and luminal pressure control lateral branching morphogenesis in the embryonic avian lung.","authors":"Shelby R Mohr-Allen, Jason P Gleghorn, Victor D Varner","doi":"10.1016/j.ydbio.2025.01.016","DOIUrl":"10.1016/j.ydbio.2025.01.016","url":null,"abstract":"During lung development, the embryonic airway originates as a wishbone-shaped epithelial tube, which undergoes a series of branching events to build the bronchial tree. This process depends crucially on cell proliferation and is thought to involve distinct branching modes: lateral branching, wherein daughter branches emerge along the length of a parent branch, and bifurcations, wherein the tip of a parent branch splits to form two new daughter branches. The developing airway is fluid-filled, and previous studies have shown that altered luminal pressure can influence rates of branching morphogenesis. However, it is not clear if altered tissue mechanics influence patterns of proliferation along the embryonic airway epithelium nor if individual branching modes are affected differently by changes in luminal pressure. Here, we focused on mechanisms of lateral branching and used as a model system the embryonic avian lung, which forms exclusively via this branching mode during early development. We used microinjected fluid droplets or pharmacological modulators of fluid secretion to alter luminal fluid pressure either locally or globally within cultured embryonic lungs. Somewhat surprisingly, we found both local and global increases in luminal pressure to suppress the formation of new lateral branches while also promoting increased epithelial proliferation. In a consistent manner, decreased luminal pressure led to an increase in lateral branching morphogenesis. Morphometric analysis of airway branching patterns revealed that altered luminal pressure shifts the overall branching program, rather than simply changing rates of morphogenesis. Taken together, these results highlight the importance of mechanical forces during airway branching and suggest that different branching modes may be affected differently by luminal fluid pressure.","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":" ","pages":"251-263"},"PeriodicalIF":2.5,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143051968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual role of Xenopus Odf2 in multiciliated cell patterning and differentiation.

IF 2.5 3区生物学

Developmental biology Pub Date : 2025-01-24 DOI: 10.1016/j.ydbio.2025.01.014

Aude Nommick, Alexandre Chuyen, Raphael Clément, Virginie Thomé, Fabrice Daian, Olivier Rosnet, Fabrice Richard, Nicolas Brouilly, Etienne Loiseau, Camille Boutin, Laurent Kodjabachian

{"title":"Dual role of Xenopus Odf2 in multiciliated cell patterning and differentiation.","authors":"Aude Nommick, Alexandre Chuyen, Raphael Clément, Virginie Thomé, Fabrice Daian, Olivier Rosnet, Fabrice Richard, Nicolas Brouilly, Etienne Loiseau, Camille Boutin, Laurent Kodjabachian","doi":"10.1016/j.ydbio.2025.01.014","DOIUrl":"10.1016/j.ydbio.2025.01.014","url":null,"abstract":"In developing tissues, the number, position, and differentiation of cells must be coordinately controlled to ensure the emergence of physiological function. The epidermis of the Xenopus embryo contains thousands of uniformly distributed multiciliated cells (MCCs), which grow hundreds of coordinately polarized cilia that beat vigorously to generate superficial water flow. Using this model, we uncovered a dual role for the conserved centriolar component Odf2, in MCC apical organization at the cell level, and in MCC spatial distribution at the tissue level. Like in other species, Xenopus Odf2 localized to the basal foot of basal bodies. Consistently, Odf2 morpholino-mediated knockdown impaired basal foot morphogenesis. Consequently, the rate of microtubule nucleation by Odf2-deficient basal bodies was reduced, leading to cilia disorientation, reduced beating, and ultimately altered flow production across the embryo. Furthermore, we show that Odf2 is required to maintain MCC motility and homotypic repulsion prior to their emergence into the surface layer. Our data suggest that Odf2 promotes MCC spacing via its role in the modulation of cytoplasmic microtubule dynamics. Mathematical simulations confirmed that reduced migration speed alters the spacing order of MCCs. This study provides a striking example of coupling between organizational scales by a unique effector.","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":" ","pages":"224-238"},"PeriodicalIF":2.5,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143045929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shh signaling directs dorsal ventral patterning in the regenerating X. tropicalis spinal cord.

IF 2.5 3区生物学

Developmental biology Pub Date : 2025-01-22 DOI: 10.1016/j.ydbio.2025.01.015

Avery Angell Swearer, Samuel Perkowski, Andrea Wills

{"title":"Shh signaling directs dorsal ventral patterning in the regenerating X. tropicalis spinal cord.","authors":"Avery Angell Swearer, Samuel Perkowski, Andrea Wills","doi":"10.1016/j.ydbio.2025.01.015","DOIUrl":"10.1016/j.ydbio.2025.01.015","url":null,"abstract":"Tissue development and regeneration rely on the deployment of embryonic signals to drive progenitor activity and thus generate complex cell diversity and organization. One such signal is Sonic Hedgehog (Shh), which establishes the dorsal-ventral (D/V) axis of the spinal cord during embryogenesis. However, the existence of this D/V axis and its dependence on Shh signaling during regeneration varies by species. Here we investigate the function of Shh signaling in patterning the D/V axis during spinal cord regeneration in Xenopus tropicalis tadpoles. We find that neural progenitor markers Msx1/2, Nkx6.1, and Nkx2.2 are confined to dorsal, intermediate and ventral spatial domains, respectively, in both the uninjured and regenerating spinal cord. These domains are altered by perturbation of Shh signaling. Additionally, we find that these D/V domains are more sensitive to Shh perturbation during regeneration than uninjured tissue. The renewed sensitivity of these neural progenitor cells to Shh signals represents a regeneration specific response and raises questions about how responsiveness to developmental patterning cues is regulated in mature and regenerating tissues.","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":" ","pages":"191-199"},"PeriodicalIF":2.5,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143037356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

At early stages of heart development, the first and second heart fields are a continuum of lateral head mesoderm-derived, cardiogenic cells.

IF 2.5 3区生物学

Developmental biology Pub Date : 2025-01-21 DOI: 10.1016/j.ydbio.2025.01.009

Matthew Wolton, Megan G Davey, Susanne Dietrich

{"title":"At early stages of heart development, the first and second heart fields are a continuum of lateral head mesoderm-derived, cardiogenic cells.","authors":"Matthew Wolton, Megan G Davey, Susanne Dietrich","doi":"10.1016/j.ydbio.2025.01.009","DOIUrl":"https://doi.org/10.1016/j.ydbio.2025.01.009","url":null,"abstract":"Pioneering work in the chicken established that the initial development of the heart consists of two stages: the quick assembly of a beating heart, followed by the recruitment of cells from adjacent tissues to deliver the mature in-and outflow tract. Cells to build the primitive heart were dubbed the first heart field (FHF) cells, cells to be recruited later the second heart field (SHF) cells. The current view is that these cells represent distinct, maybe even pre-determined lineages. However, it is still unclear where exactly FHF and SHF are located at different stages of development, and whether there is a sharp boundary or rather an overlap between the two. It is also unclear whether both FHF cells and SHF cells originate from the lateral head mesoderm (LHM), whether the paraxial head mesoderm (PHM) contributes to the SHF, and where the LHM-PHM boundary might be. To investigate this problem, we exploited the size, ease of access and exquisite anatomy of the chicken embryo and used traditional strategies as well as newly developed transgenic lines to trace the location of cardiogenic fields and boundaries from the time the first heart-markers are expressed to the time SHF cell recruitment ceases. Our work shows that both FHF and SHF stem from the LHM. We also found that FHF and SHF lack a distinct anatomical boundary. Rather, FHF and SHF are a continuum, and the recruitment of cells into the heart is a chance event depending on morphogenetic movements, the position of cells within the moving tissues, the separation of the somatic and splanchnic LHM, and the separation of the heart from the splanchnic subpharyngeal mesoderm during heart-looping. Reconciling our and previous studies we propose that first and second heart field precursors are specified but not determined, thus relying on morphogenetic processes and local environments to realise their cardiogenic potential.","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Naturally occurring, rostrally conjoining chicken twins attempt to make a forebrain.

IF 2.5 3区生物学

Developmental biology Pub Date : 2025-01-21 DOI: 10.1016/j.ydbio.2025.01.013

Frank R Schubert, Susanne Dietrich

{"title":"Naturally occurring, rostrally conjoining chicken twins attempt to make a forebrain.","authors":"Frank R Schubert, Susanne Dietrich","doi":"10.1016/j.ydbio.2025.01.013","DOIUrl":"10.1016/j.ydbio.2025.01.013","url":null,"abstract":"Conjoined twinning is a special case of monozygotic, monoamniotic twinning. Human conjoined twinning, and vertebrate conjoined twinning in general, is a very rare phenomenon. It has been suggested that the risk of conjoined twinning increases with some medication and upon assisted reproduction. Survival rates are low. When conjoined twins occur in the chicken, they most often present with fused heads, anatomically unrecognisable brains and two normal bodies. Recent studies suggested that forebrain, midbrain and rostral hindbrain identities are established in the early epiblast before neural induction and independent from caudal hindbrain and spinal cord identities. Therefore, it is unclear whether in conjoined twins, the aberrant brain anatomy is a result of the rostral fusion, or whether the brains failed to develop in the first place. Here, we collected conjoined twins as they spontaneously appeared in eggs incubated for stages HH4 (late primitive steak stage) to HH13 (early pharyngula). The twinned embryos and stage-matched normal embryos were analysed for the expression of the rostral epiblast and forebrain-midbrain marker Otx2 and the ventral forebrain marker Six3. We found normal anatomy and marker gene expression that lasted up to stage HH9. By HH12-13, the brain anatomy had deteriorated, but marker genes remained expressed. This suggests that the fusing embryos attempted to generate a brain including the forebrain. Besides addressing forebrain development, our work for the first time provides a time frame to study the mechanisms underlying the interaction and fusion of conjoined twins, which will pave the way to a better understanding and management of risk factors in humans.","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":" ","pages":"171-179"},"PeriodicalIF":2.5,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Loss of KAT6B causes premature ossification and promotes osteoblast differentiation during development. KAT6B缺失会导致过早骨化，并在发育过程中促进成骨细胞分化。

IF 2.5 3区生物学

Developmental biology Pub Date : 2025-01-19 DOI: 10.1016/j.ydbio.2025.01.012

Maria I Bergamasco, Jacqueline M Ogier, Alexandra L Garnham, Lachlan Whitehead, Kelly Rogers, Gordon K Smyth, Rachel A Burt, Anne K Voss, Tim Thomas

{"title":"Loss of KAT6B causes premature ossification and promotes osteoblast differentiation during development.","authors":"Maria I Bergamasco, Jacqueline M Ogier, Alexandra L Garnham, Lachlan Whitehead, Kelly Rogers, Gordon K Smyth, Rachel A Burt, Anne K Voss, Tim Thomas","doi":"10.1016/j.ydbio.2025.01.012","DOIUrl":"10.1016/j.ydbio.2025.01.012","url":null,"abstract":"The MYST family histone acetyltransferase gene, KAT6B (MYST4, MORF, QKF) is mutated in two distinct human congenital disorders characterised by intellectual disability, facial dysmorphogenesis and skeletal abnormalities; the Say-Barber-Biesecker-Young-Simpson variant of Ohdo syndrome and Genitopatellar syndrome. Despite its requirement in normal skeletal development, the cellular and transcriptional effects of KAT6B in skeletogenesis have not been thoroughly studied. Here, we show that germline deletion of the Kat6b gene in mice causes premature ossification in vivo, resulting in shortened craniofacial elements and increased bone density, as well as shortened tibias with an expanded pre-hypertrophic layer, as compared to wild type controls. Mechanistically, we show that the loss of KAT6B in mesenchymal progenitor cells promotes transition towards an osteoblast-progenitor state with upregulation of gene targets of RUNX2, a master regulator of osteoblast development and concomitant downregulation of SOX9, a critical gene in chondrocyte development. Moreover, we find that compound heterozygosity at Kat6b and Runx2 loci partially rescues the reduction in ossification of Runx2 heterozygous, but not homozygous mice, suggesting that KAT6B may limit the action of RUNX2, possibly through a role in maintaining progenitors in an undifferentiated state. Moreover, our results show that KAT6B has essential roles in regulating the expression of a large number of genes involved in skeletogenesis and bone development.","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":" ","pages":"141-154"},"PeriodicalIF":2.5,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Outside Back Cover - Graphical abstract TOC/TOC in double column/Cover image legend if applicable, Bar code, Abstracting and Indexing information

IF 2.5 3区生物学

Developmental biology Pub Date : 2025-01-18 DOI: 10.1016/S0012-1606(25)00019-3

引用次数: 0

The role of serendipity in our investigation of embryo implantation. 偶然性在胚胎着床研究中的作用。

IF 2.5 3区生物学

Developmental biology Pub Date : 2025-01-16 DOI: 10.1016/j.ydbio.2025.01.010

Ann E Sutherland

引用次数: 0

Mammalian lactation as a framework for teaching development, physiology, and cell biology for social change. 哺乳动物哺乳作为教学发展的框架，生理学和细胞生物学的社会变革。

IF 2.5 3区生物学

Developmental biology Pub Date : 2025-01-16 DOI: 10.1016/j.ydbio.2025.01.011

Melissa E Marks, Emma J Coddington Brown

{"title":"Mammalian lactation as a framework for teaching development, physiology, and cell biology for social change.","authors":"Melissa E Marks, Emma J Coddington Brown","doi":"10.1016/j.ydbio.2025.01.011","DOIUrl":"10.1016/j.ydbio.2025.01.011","url":null,"abstract":"Mammalian lactation is a dynamic process that develops throughout the lifespan of an organism. Here we present a framework for a third semester core course in biology that centers course content on lactation allowing examination of the developmental process as a dynamic whole-body experience involving changes occurring at the molecular, cellular, and organ levels of organization. Inequitable economic, socio- and geopolitical systems structure social determinants of health, affecting rates of breastfeeding in human populations. By integrating content exploring the ways social and biological systems impact breastfeeding rates in human populations, students develop abilities to understand the relationship between science and society throughout the course, a critical core competency for engaging in social change. Importantly, they interrogate social systems while simultaneously learning about many canonical biological processes including how natural selection and constraint have shaped the anatomy, physiology, cell biology, and biochemistry of lactation, how proteins, lipids, and carbohydrates are synthesized, processed, and exported through the endomembrane system in eukaryotes, and how neuronal and hormonal feedback mechanisms regulate milk synthesis and secretion. The course is structured using a flipped-classroom design emphasizing revision and student-self assessment that supports development of biological knowledge, social responsibility, and metacognitive skills. Because mammalian lactation includes fascinating, nuanced, and complex components that cross interdisciplinary boundaries, it provides a wealth of opportunities for faculty to teach developmental biology for social change.","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":" ","pages":"180-190"},"PeriodicalIF":2.5,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0