Developmental biology最新文献

Shrew is an essential, recently evolved, Drosophila specific gene required for formation of the embryonic BMP activity gradient 鼩鼱是一个重要的，最近进化的，果蝇特定的基因需要形成胚胎BMP活性梯度。

IF 2.1 3区生物学

Developmental biology Pub Date : 2026-06-01 Epub Date: 2026-03-04 DOI: 10.1016/j.ydbio.2026.03.001

MaryJane Shimell , Robert Connacher , Sangbin Park , Aidan J. Peterson , Kavita Arora , Michael B. O'Connor

{"title":"Shrew is an essential, recently evolved, Drosophila specific gene required for formation of the embryonic BMP activity gradient","authors":"MaryJane Shimell , Robert Connacher , Sangbin Park , Aidan J. Peterson , Kavita Arora , Michael B. O'Connor","doi":"10.1016/j.ydbio.2026.03.001","DOIUrl":"10.1016/j.ydbio.2026.03.001","url":null,"abstract":"<div><div>Patterning the dorsal surface of the Drosophila blastoderm embryo requires the rapid redistribution of a BMP heterodimer (Decapentaplegic/Screw) from lateral regions of the embryo to the dorsal midline as cellularization is completed. BMP redistribution creates a steeply graded signal with peak activity in the mid-dorsal region and requires four additional secreted gene products: Short gastrulation (Sog), Twisted gastrulation (Tsg), Tolloid (Tld), and Shrew (Srw). While the functions of Sog, Tsg, Tld, and their vertebrate homologs, in generating BMP activity gradients have been well-described, the role of Drosophila Srw remains an enigma. Here we show that Srw encodes a secreted, N-terminally truncated paralog of Tsg that, like Tsg, stimulates the cleavage of Sog by Tld. However, unlike Tsg, it does not form a stable interaction with any identified component of the patterning machinery. Structural modeling and mutant analysis suggest Srw transiently binds to the Sog/Tsg/BMP complex to facilitate proteolysis of Sog. We further demonstrate that the requirement for Srw can be bypassed by providing additional Tsg in <em>srw</em> mutant embryos prior to cellularization. Phylogenetic analysis suggests that Tsg and Srw emerged from separate duplications of the Crossveinless (Cv) paralog in higher Diptera, and then Drosophilid lineages respectively, thus providing components for progressive specialization of insect embryo patterning. Taking into consideration our genetic, biochemical and phylogenetic data, we present three possible mechanistic models for how Srw might accelerate Sog cleavage by Tld, to hasten BMP gradient formation in rapidly developing Drosophila embryos.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"534 ","pages":"Pages 9-22"},"PeriodicalIF":2.1,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147369480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cortical hem-like progenitors contribute pyramidal neurons to the neocortex and hippocampus 皮层边缘样祖细胞为新皮层和海马提供锥体神经元。

IF 2.1 3区生物学

Developmental biology Pub Date : 2026-06-01 Epub Date: 2026-02-28 DOI: 10.1016/j.ydbio.2026.02.012

Arpan Parichha , Patrick Azzam , Frederic Causeret , Suranjana Pal , Elizabeth Ann Grove , Angeliki Louvi , Alessandra Pierani , Shubha Tole

引用次数: 0

Pre-fertilization Egg Coat Dynamics in Phallusia philippinensis (Tunicata, Ascidiacea). 菲律宾阳虱（被毛纲，海鞘纲）受精前卵皮动态。

IF 2.1 3区生物学

Developmental biology Pub Date : 2026-05-05 DOI: 10.1016/j.ydbio.2026.05.002

Kana Hanazaki, Noburu Sensui, Keita Kinjo, Madoka Sano, Takumi T Shito, Euichi Hirose, Takako Saito

{"title":"Pre-fertilization Egg Coat Dynamics in Phallusia philippinensis (Tunicata, Ascidiacea).","authors":"Kana Hanazaki, Noburu Sensui, Keita Kinjo, Madoka Sano, Takumi T Shito, Euichi Hirose, Takako Saito","doi":"10.1016/j.ydbio.2026.05.002","DOIUrl":"https://doi.org/10.1016/j.ydbio.2026.05.002","url":null,"abstract":"<p><p>In externally fertilizing species such as ascidians, gametes are released into the surrounding seawater, which makes the fertilization process highly sensitive to environmental conditions. In the ascidian Phallusia philippinensis, the oviductal fluid has an acidic pH of 5.65 (Sensui et al., 2023), markedly lower than that of seawater (approximately 8.2). Consequently, eggs experience a rapid and substantial pH shift when released into the external environment. The vitelline coat (VC), an extracellular matrix surrounding the oocyte, serves both as a structural barrier and as a mediator of essential sperm-egg interactions during fertilization. In this study, we report for the first time a pH-dependent, pre-fertilization elevation of the VC in the ascidian P. philippinensis. The eggs of P. philippinensis exhibit rapid VC elevation that occurs independently of fertilization after release into seawater. This process appears to be driven by osmotic pressure and enzymatic structural loosening of the VC, rather than cortical granule exocytosis, which is well known to initiate the modification and hardening of the fertilization envelope in organisms such as sea urchins and mammals. Consistent with this, VC elevation occurs independently of fertilization and does not function as a polyspermy block in this species. These findings reveal a previously unrecognized pH-responsive property of the ascidian egg coat and provide new insights into a seawater pH-triggered mechanism regulating egg coat structure.</p>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147835028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-neural crest lineage contributions to the enteric nervous system: Moving a 50 year conclusion. 非神经嵴谱系对肠神经系统的贡献：移动50年的结论。

IF 2.1 3区生物学

Developmental biology Pub Date : 2026-05-04 DOI: 10.1016/j.ydbio.2026.05.001

Henry M Sucov, Takako Makita

{"title":"Non-neural crest lineage contributions to the enteric nervous system: Moving a 50 year conclusion.","authors":"Henry M Sucov, Takako Makita","doi":"10.1016/j.ydbio.2026.05.001","DOIUrl":"10.1016/j.ydbio.2026.05.001","url":null,"abstract":"<p><p>It is considered an established fact that the entirety of the autonomic nervous system, including the enteric nervous system (ENS), is derived from neural crest. This conclusion is based on classical quail-chick grafting studies conducted by Le Douarin and colleagues in the 1970s, and results consistent with this conclusion have been obtained in mouse using recombinase-based neural crest-specific lineage tracing and gene knockout. Recently, additional sources that also contribute to the ENS have been implicated. Because these observations run counter to the neural crest-exclusive view, here we revisit the original avian studies by Le Douarin and subsequent mouse studies that in fact did not exclude additional lineage contributions to the ENS, particularly in the distal colon. We also review the evidence from mice supporting an ENS contribution from several non-neural crest lineages, including the placodes for which the evidence for contribution to the colonic ENS is particularly strong. Hirschsprung disease is a severe condition in which the colonic ENS is deficient at birth, and cell transplantation strategies for Hirschsprung disease are in many cases predicated on the assumption that all of the missing ENS components are neural crest-derived. Thus, beyond the general interest in this fundamental feature of developmental biology, it is particularly important that investigators who pursue cell transplantation approaches for Hirschsprung disease or therapeutic approaches for any ENS-associated condition take these observations into consideration.</p>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":" ","pages":"85-94"},"PeriodicalIF":2.1,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147835044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fibroblast Growth Factor 8 Regulates Early Meckel's Cartilage Development via ERK signaling in a Stage- and Dose-Dependent Manner. 成纤维细胞生长因子8通过ERK信号以阶段和剂量依赖的方式调节早期梅克尔软骨发育。

IF 2.1 3区生物学

Developmental biology Pub Date : 2026-05-02 DOI: 10.1016/j.ydbio.2026.04.007

He Qi, Fumie Terao, Diyaa Al Akel, Keigo Yoshizaki, Ichiro Takahashi

{"title":"Fibroblast Growth Factor 8 Regulates Early Meckel's Cartilage Development via ERK signaling in a Stage- and Dose-Dependent Manner.","authors":"He Qi, Fumie Terao, Diyaa Al Akel, Keigo Yoshizaki, Ichiro Takahashi","doi":"10.1016/j.ydbio.2026.04.007","DOIUrl":"https://doi.org/10.1016/j.ydbio.2026.04.007","url":null,"abstract":"<p><p>Precise spatiotemporal regulation of epithelial-mesenchymal interactions is fundamental to craniofacial morphogenesis. Fibroblast Growth Factor 8 (FGF8) is a pivotal signaling molecule in early mandibular development; however, its stage-specific regulatory mechanisms remain poorly understood. In this study, we demonstrate that Fgf8 and its receptors exhibit a dynamic, declining expression profile in the mouse mandibular arch between embryonic days 9 (E9) and E11. Using organ and micromass culture systems, we identify a critical developmental window at E10 during which FGF8 exerts a dual effect: it promotes mesenchymal cell expansion while simultaneously suppressing chondrogenic differentiation in a dose-dependent manner. Interestingly, by E11, mandibular cells undergo an autonomous transcriptional shift, reduced proliferative capacity, and show downregulation of proliferation-related gene sets, leading to resistance to FGF8-induced proliferation and chondrogenic inhibition. Mechanistically, we show that FGF8 specifically activates the ERK signaling pathway within the responsive E10 window. Pharmacological inhibition of MEK/ERK signaling successfully rescues the suppressed chondrogenic differentiation, identifying ERK as the key mediator of FGF8-induced chondrogenic inhibition at this stage. Together, our findings reveal that FGF8 acts as a stage-specific regulator that coordinates the transition from cell proliferation to chondrogenic commitment during early Meckel's cartilage development via the ERK pathway.</p>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147835012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Crosstalk between corticosterone and triiodothyronine signaling in Xenopus tropicalis hindlimb buds 热带爪蟾后肢芽中皮质酮和三碘甲状腺原氨酸信号的串扰。

IF 2.1 3区生物学

Developmental biology Pub Date : 2026-05-01 Epub Date: 2026-02-02 DOI: 10.1016/j.ydbio.2026.02.001

Alexis Grimaldi , Muriel Rigolet , Corinne Blugeon , Nicolas Buisine , Laurent M. Sachs

{"title":"Crosstalk between corticosterone and triiodothyronine signaling in Xenopus tropicalis hindlimb buds","authors":"Alexis Grimaldi , Muriel Rigolet , Corinne Blugeon , Nicolas Buisine , Laurent M. Sachs","doi":"10.1016/j.ydbio.2026.02.001","DOIUrl":"10.1016/j.ydbio.2026.02.001","url":null,"abstract":"<div><div>Thyroid hormone signaling has a wide range of biological effects involved in the control of homeostasis and development. In amphibian, thyroid hormones control the onset of metamorphosis, which resemble the mammalian perinatal period. Glucocorticoids too are involved in vertebrate development but they also mediate stress response and thus act as a relay that channel environmental inputs and modulate biological processes. Despite being deeply adaptive, glucocorticoids responses may nonetheless promote more detrimental long-term effects because of sub-optimal benefit-cost balances. To date, the extent and the diversity of functional interactions between thyroid hormone and glucocorticoid signaling are supported by very little molecular data. Here, we used transcriptomic analysis to collect the molecular details of the crosstalk between triiodothyronine and corticosterone signaling during limb development, <em>in vivo</em>. We show that the transcriptional responses to the two hormones are highly archetypal and that crosstalk affect expression of a few hundred of genes, with some of them potentially involved in bone development.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"533 ","pages":"Pages 1-15"},"PeriodicalIF":2.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146118070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of zygotic arrest genes in female meiosis and early embryonic development. 合子抑制基因在雌性减数分裂和早期胚胎发育中的作用。

IF 2.1 3区生物学

Developmental biology Pub Date : 2026-05-01 DOI: 10.1016/j.ydbio.2026.04.019

Srija Sadhukhan, Kousik Pramanick

{"title":"Role of zygotic arrest genes in female meiosis and early embryonic development.","authors":"Srija Sadhukhan, Kousik Pramanick","doi":"10.1016/j.ydbio.2026.04.019","DOIUrl":"10.1016/j.ydbio.2026.04.019","url":null,"abstract":"<p><p>Zygotic arrest genes play an indispensable role in regulating the critical transition from maternal to zygotic control during early embryonic development, commonly referred to as zygotic genome activation (ZGA). These genes orchestrate fundamental processes such as maternal mRNA clearance, chromatin remodelling, epigenetic reprogramming, and transcriptional activation, ensuring the developmental competence of embryos. Key genes, including ZAR1, ZAR2, BTG4, NPM2, PADI6, and KHDC3, have been identified as essential regulators of oocyte maturation, meiotic progression, and cleavage-stage development. Dysregulation or mutations in these genes often result in embryonic arrest at the 1-cell, 2-cell, or 4-cell stage, with profound implications for fertility and reproductive success in both humans and animal models. Recent advances in experimental approaches, including gene knockout/knockdown studies, RNA sequencing, transcriptomic profiling, and CRISPR-Cas9 genome editing, have significantly enhanced our understanding of zygotic arrest gene function. Clinical studies have further linked specific mutations to recurrent infertility and poor outcomes in assisted reproductive technologies (ART), such as in vitro fertilisation (IVF) and intracytoplasmic sperm injection (ICSI). These findings underscore the translational potential of zygotic arrest gene research, with prospects for genetic screening, personalized treatment strategies, and novel therapeutic targets. Future directions emphasize integrative omics approaches, cross-species comparative studies, and ethical considerations in embryo manipulation, aiming to bridge fundamental developmental biology with clinical reproductive medicine. Collectively, zygotic arrest genes represent a vital nexus between basic science and translational applications, holding promise for improving fertility outcomes and advancing reproductive health.</p>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":" ","pages":"32-40"},"PeriodicalIF":2.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147811903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FGF signalling orchestrates multiple roles during salivary gland branching morphogenesis. FGF信号在唾液腺分支形态发生过程中发挥着多种作用。

IF 2.1 3区生物学

Developmental biology Pub Date : 2026-04-29 DOI: 10.1016/j.ydbio.2026.04.008

Marta Perera, Decheng Kong, Joshua M Brickman, Abigail S Tucker

{"title":"FGF signalling orchestrates multiple roles during salivary gland branching morphogenesis.","authors":"Marta Perera, Decheng Kong, Joshua M Brickman, Abigail S Tucker","doi":"10.1016/j.ydbio.2026.04.008","DOIUrl":"10.1016/j.ydbio.2026.04.008","url":null,"abstract":"<p><p>The function of the organs in our body is intrinsically related to their form, acquired during embryonic development. Many epithelial organs such as lung, kidney, pancreas or salivary glands branch during morphogenesis to optimise the available surface of the epithelium. In the embryo, Fibroblast Growth Factor (FGF) ligands secreted by the surrounding mesenchyme control both elongation and branching of the epithelium. However, the intracellular pathways activated downstream of the receptor and the specific transcriptional changes that occur in the epithelial cells upon signalling are not completely understood. Using the murine salivary gland as a model of branching morphogenesis, we have taken advantage of its ease of ex vivo culture to identify the molecular players downstream of FGF during branching. We compared the morphological and transcriptional changes that occurred after pharmaceutical inhibition vs genetic deletion of FGF receptors and identified a dependence of FGF for the correct coordination of nerve and blood vessel growth with the budding gland epithelium. Furthermore, we identified a role for FGF in establishing cell-cell junctions, crucial for epithelium architecture and integrity. Importantly, FGF signalling via ERK was shown to be key for upregulation of the mature bud cell marker cKIT, suggesting FGF could influence cell fate decisions during gland development. Altogether, our approach uncovers the many roles of FGF signalling in salivary gland branching morphogenesis.</p>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":" ","pages":"70-84"},"PeriodicalIF":2.1,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147811868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RNA-binding proteins in the mouse lens: Functional classifications, expression profiling, and interaction studies of Carhsp1 with crystallin mRNAs. 小鼠晶状体中的rna结合蛋白：Carhsp1与晶体蛋白mrna的功能分类、表达谱和相互作用研究。

IF 2.1 3区生物学

Developmental biology Pub Date : 2026-04-28 DOI: 10.1016/j.ydbio.2026.04.017

Danielle Rayêe, Dong-Woo Hwang, William K Chang, Ilana N Karp, Sarah Y Coomson, Yilin Zhao, Teresa Bowman, Salil A Lachke, Robert H Singer, Carolina Eliscovich, Ales Cvekl

{"title":"RNA-binding proteins in the mouse lens: Functional classifications, expression profiling, and interaction studies of Carhsp1 with crystallin mRNAs.","authors":"Danielle Rayêe, Dong-Woo Hwang, William K Chang, Ilana N Karp, Sarah Y Coomson, Yilin Zhao, Teresa Bowman, Salil A Lachke, Robert H Singer, Carolina Eliscovich, Ales Cvekl","doi":"10.1016/j.ydbio.2026.04.017","DOIUrl":"10.1016/j.ydbio.2026.04.017","url":null,"abstract":"<p><p>RNA-binding proteins (RBPs) are critical regulators of mRNAs controlling all processes such as RNA transcription, transport, localization, translation, mRNA:ncRNA interactions, and decay. Cellular differentiation is driven by temporally and spatially regulated expression of proteins needed for the optimal function of individual cells, tissues and organs. Lens fiber cell differentiation is marked by high levels of expression of crystallin genes encoding critical proteins for lens transparency and light refraction. Herein we performed proteomic and transcriptomic analyses of RBPs in differentiating mouse lenses to identify the most abundant RBPs and establish dynamic changes of their expression in differentiating lenses. Expression analyses showed highly abundant RBPs, including Carhsp1, Igf2bp1/ZBP1, Ybx1, Pabpc1, Ddx39, and Rbm38. Binding sites of Carhsp1, the most abundant lens RBP, were predicted in various crystallin and β-actin mRNAs. Immunoprecipitations using Carhsp1-specific antibodies confirmed interactions of Carhsp1 with crystallin mRNAs in newborn lens. A combination of single molecule RNA FISH (smFISH) and immunofluorescence was used to probe in vivo interactions of Carhsp1 with αA-, αB-crystallin, and β-actin mRNAs in cytoplasm and nucleoplasm of cultured mouse lens epithelial cells. These experiments favor a working model of direct association of Carhsp1 mediated by multiple candidate binding sites within both αA-, αB-crystallin mRNAs. Together, these results open new avenues to perform comprehensive genetic, cell, and molecular biology studies of individual RBPs in the lens.</p>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":" ","pages":"14-31"},"PeriodicalIF":2.1,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147811839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The chicken embryo as a model for developmental genomics. 鸡胚作为发育基因组学的模型。

IF 2.1 3区生物学

Developmental biology Pub Date : 2026-04-24 DOI: 10.1016/j.ydbio.2026.04.016

Nagif Alata-Jimenez, Marcos Simoes-Costa

{"title":"The chicken embryo as a model for developmental genomics.","authors":"Nagif Alata-Jimenez, Marcos Simoes-Costa","doi":"10.1016/j.ydbio.2026.04.016","DOIUrl":"10.1016/j.ydbio.2026.04.016","url":null,"abstract":"<p><p>The chicken embryo has been a central model in developmental biology for centuries, prized for its experimental tractability and the ease with which embryonic tissues can be directly observed and manipulated. Its external development allows precise perturbations of cell fate, signaling, and morphogenesis that have defined core principles of vertebrate development. These strengths have taken on new significance with the advent of genomic technologies, which have largely offset the absence of robust forward genetics in the chick, repositioning it as a powerful platform for functional and regulatory genomics. The sequencing of the chicken genome, combined with high-throughput approaches to profile gene expression, chromatin accessibility, transcription factor binding, and genome organization, has opened new avenues for interrogating gene regulatory mechanisms directly in vivo. The chick is especially well-positioned for such studies: its compact genome supports high-quality genomic assays, its experimental throughput enables systematic perturbation, and its evolutionary placement among vertebrates makes it valuable for comparative analyses. In this review, we discuss how genomic and epigenomic approaches have reshaped the use of the chick embryo, highlighting advances in genome organization, regulatory element function, and comparative genomics, as well as the experimental toolkit that enables mechanistic studies of developmental gene regulation.</p>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":" ","pages":"41-55"},"PeriodicalIF":2.1,"publicationDate":"2026-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147765298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0