Developmental biology最新文献

{"title":"Dr. Igor B. Dawid (1935–2024)","authors":"Brant M. Weinstein ,&nbsp;Reiko Toyama ,&nbsp;Michael Tsang ,&nbsp;Neil A. Hukriede","doi":"10.1016/j.ydbio.2024.05.008","DOIUrl":"10.1016/j.ydbio.2024.05.008","url":null,"abstract":"<div><p>Renowned developmental biologist Dr. Igor Dawid passed away on February 13, 2024.</p></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141047643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The development of hair follicles and nail","authors":"Soung-Hoon Lee ,&nbsp;Sarah Platt ,&nbsp;Chae Ho Lim ,&nbsp;Mayumi Ito ,&nbsp;Peggy Myung","doi":"10.1016/j.ydbio.2024.05.010","DOIUrl":"10.1016/j.ydbio.2024.05.010","url":null,"abstract":"<div><p>The hair follicle and nail unit develop and regenerate through epithelial-mesenchymal interactions. Here, we review some of the key signals and molecular interactions that regulate mammalian hair follicle and nail formation during embryonic development and how these interactions are reutilized to promote their regeneration during adult homeostasis and in response to skin wounding. Finally, we highlight the role of some of these signals in mediating human hair follicle and nail conditions.</p></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140956777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"David L. Stocum (1939–2023): Authority in regenerative biology, passionate educator, visionary administrative leader, and cherished colleague and friend","authors":"Günther K.H. Zupanc","doi":"10.1016/j.ydbio.2024.05.009","DOIUrl":"10.1016/j.ydbio.2024.05.009","url":null,"abstract":"","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140956773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hedgehog signaling is required for larval muscle development and larval metamorphosis of the mussel Mytilus coruscus","authors":"Yi Tang ,&nbsp;Yu-Qing Wang ,&nbsp;Ji-Yue Ni ,&nbsp;Yue-Tong Lin ,&nbsp;Yi-Feng Li","doi":"10.1016/j.ydbio.2024.05.007","DOIUrl":"10.1016/j.ydbio.2024.05.007","url":null,"abstract":"<div><p>Understanding the developmental processes and signaling pathways involved in larval myogenesis and metamorphosis is crucial for comprehending the life history and adaptive strategies of marine organisms. In this study, we investigated the temporal and spatial patterns of myogenesis in the mussel <em>Mytilus coruscus</em> (Mc), focusing on the emergence and transformation of major muscle groups during different larval stages. We also explored the role of the Hedgehog (Hh) signaling pathway in regulating myogenesis and larval metamorphosis. The results revealed distinct developmental stages characterized by the emergence of specific muscular components, such as velum retractor muscles and anterior adductor muscles, in D-veliger and umbo larvae, which are responsible for the planktonic stage. In the pediveliger stage, posterior ventral, posterior adductor, and foot muscles appeared. After larval metamorphosis, the velum structure and its corresponding retractor muscles degenerate, indicating the transition from planktonic to benthic life. We observed a conserved pattern of larval musculature development and revealed a high degree of conservation across bivalve species, with comparable emergence times during myogenesis. Furthermore, exposure to the Hh signaling inhibitor cyclopamine impaired larval muscle development, reduced larval swimming activity, and inhibited larval metamorphosis in <em>M. coruscus</em>. Cyclopamine-mediated inhibition of Hh signaling led to reduced expression of four key genes within the Hh signaling pathway (<em>McHh</em>, <em>McPtc</em>, <em>McSmo</em>, and <em>McGli</em>) and the striated myosin heavy chain gene (<em>McMHC</em>). It is hypothesised that the abnormal larval muscle development in cyclopamine-treated groups may be an indirect effect due to disrupted <em>McMHC</em> expression. We provide evidence for the first time that cyclopamine treatment inhibited larval metamorphosis in bivalves, highlighting the potential involvement of Hh signaling in mediating larval muscle development and metamorphosis in <em>M. coruscus</em>. The present study provides insights into the dynamic nature of myogenesis and the regulatory role of the Hh signaling pathway during larval development and metamorphosis in <em>M. coruscus</em>. The results obtained in this study contribute to a better understanding of the evolutionary significance of Hh signaling in bivalves and shed light on the mechanisms underlying larval muscle development and metamorphosis in marine invertebrates.</p></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140944531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacological reprogramming of zebrafish lateral line supporting cells to a migratory progenitor state","authors":"Paige M. Brooks,&nbsp;Parker Lewis,&nbsp;Sara Million-Perez,&nbsp;Anastasia S. Yandulskaya,&nbsp;Mahmoud Khalil,&nbsp;Meredith Janes,&nbsp;Joseph Porco,&nbsp;Eleanor Walker,&nbsp;Jason R. Meyers","doi":"10.1016/j.ydbio.2024.05.003","DOIUrl":"10.1016/j.ydbio.2024.05.003","url":null,"abstract":"<div><p>In the zebrafish lateral line, non-sensory supporting cells readily re-enter the cell cycle to generate new hair cells and supporting cells during homeostatic maintenance and following damage to hair cells. This contrasts with supporting cells from mammalian vestibular and auditory sensory epithelia which rarely re-enter the cell cycle, and hence loss of hair cells results in permanent sensory deficit. Lateral line supporting cells are derived from multipotent progenitor cells that migrate down the trunk midline as a primordium and are deposited to differentiate into a neuromast. We have found that we can revert zebrafish support cells back to a migratory progenitor state by pharmacologically altering the signaling environment to mimic that of the migratory primordium, with active Wnt signaling and repressed FGF signaling. The reverted supporting cells migrate anteriorly and posteriorly along the horizontal myoseptum and will re-epithelialize to form an increased number of neuromasts along the midline when the pharmacological agents are removed. These data demonstrate that supporting cells can be readily reprogrammed to a migratory multipotent progenitor state that can form new sensory neuromasts, which has important implications for our understanding of how the lateral line system matures and expands in fish and also suggest avenues for returning mammalian supporting cells back to a proliferative state.</p></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140903623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Forward genetics combined with unsupervised classifications identified zebrafish mutants affecting biliary system formation","authors":"Divya Jyoti Singh ,&nbsp;Kathryn M. Tuscano ,&nbsp;Amrhen L. Ortega ,&nbsp;Manali Dimri ,&nbsp;Kevin Tae ,&nbsp;William Lee ,&nbsp;Muslim A. Muslim ,&nbsp;Isabela M. Rivera Paz ,&nbsp;Jay L. Liu ,&nbsp;Lain X. Pierce ,&nbsp;Allyson McClendon ,&nbsp;Isabel Gibson ,&nbsp;Jodi Livesay ,&nbsp;Takuya F. Sakaguchi","doi":"10.1016/j.ydbio.2024.05.005","DOIUrl":"10.1016/j.ydbio.2024.05.005","url":null,"abstract":"<div><p>Impaired formation of the biliary network can lead to congenital cholestatic liver diseases; however, the genes responsible for proper biliary system formation and maintenance have not been fully identified. Combining computational network structure analysis algorithms with a zebrafish forward genetic screen, we identified 24 new zebrafish mutants that display impaired intrahepatic biliary network formation. Complementation tests suggested these 24 mutations affect 24 different genes. We applied unsupervised clustering algorithms to unbiasedly classify the recovered mutants into three classes. Further computational analysis revealed that each of the recovered mutations in these three classes has a unique phenotype on node-subtype composition and distribution within the intrahepatic biliary network. In addition, we found most of the recovered mutations are viable. In those mutant fish, which are already good animal models to study chronic cholestatic liver diseases, the biliary network phenotypes persist into adulthood. Altogether, this study provides unique genetic and computational toolsets that advance our understanding of the molecular pathways leading to biliary system malformation and cholestatic liver diseases.</p></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0012160624001167/pdfft?md5=4964f68238ec7ec60817decb3b85c537&pid=1-s2.0-S0012160624001167-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140903707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outside Back Cover - Graphical abstract TOC/TOC in double column/Cover image legend if applicable, Bar code, Abstracting and Indexing information","authors":"","doi":"10.1016/S0012-1606(24)00108-8","DOIUrl":"https://doi.org/10.1016/S0012-1606(24)00108-8","url":null,"abstract":"","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140893279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synchronizing Drosophila larvae with the salivary gland reporter Sgs3-GFP for discovery of phenotypes in the late third instar stage.","authors":"W. Kyle McPherson ,&nbsp;Elizabeth E. Van Gorder,&nbsp;Dalton L. Hilovsky ,&nbsp;Leila A. Jamali,&nbsp;Cami N. Keliinui,&nbsp;Miyuki Suzawa,&nbsp;Michelle L. Bland","doi":"10.1016/j.ydbio.2024.05.002","DOIUrl":"10.1016/j.ydbio.2024.05.002","url":null,"abstract":"<div><p>The larval stage of the <em>Drosophila melanogaster</em> life cycle is characterized by rapid growth and nutrient storage that occur over three instar stages separated by molts. In the third instar, the steroid hormone ecdysone drives key developmental processes and behaviors that occur in a temporally-controlled sequence and prepare the animal to undergo metamorphosis. Accurately staging <em>Drosophila</em> larvae within the final third instar is critical due to the rapid developmental progress at this stage, but it is challenging because the rate of development varies widely across a population of animals even if eggs are laid within a short period of time. Moreover, many methods to stage third instar larvae are cumbersome, and inherent variability in the rate of development confounds some of these approaches. Here we demonstrate the usefulness of the Sgs3-GFP transgene, a fusion of the Salivary gland secretion 3 (Sgs3) and GFP proteins, for staging third instar larvae. Sgs3-GFP is expressed in the salivary glands in an ecdysone-dependent manner from the midpoint of the third instar, and its expression pattern changes reproducibly as larvae progress through the third instar. We show that Sgs3-GFP can easily be incorporated into experiments, that it allows collection of developmentally-equivalent individuals from a mixed population of larvae, and that its use enables precise assessment of changing levels of hormones, metabolites, and gene expression during the second half of the third instar.</p></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140849647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leptin signaling promotes blood vessel formation in the Xenopus tail during the embryo-larval transition","authors":"Grace H. Curtis,&nbsp;Robyn E. Reeve ,&nbsp;Erica J. Crespi","doi":"10.1016/j.ydbio.2024.05.001","DOIUrl":"10.1016/j.ydbio.2024.05.001","url":null,"abstract":"<div><p>The signals that regulate peripheral blood vessel formation during development are still under investigation. The hormone leptin promotes blood vessel formation, adipose tissue establishment and expansion, tumor growth, and wound healing, but the underlying mechanisms for these actions are currently unknown. We investigated whether leptin promotes angiogenesis in the developing tail fin using embryonic transgenic xflk-1:GFP <em>Xenopus laevis,</em> which express a green fluorescent protein on vascular endothelial cells to mark blood vessels. We found that leptin protein is expressed in endothelial cells of developing blood vessels and that leptin treatment via injection increased phosphorylated STAT3 signaling, which is indicative of leptin activation of its receptor, in blood vessels of the larval tail fin. Leptin administration via media increased vessel length, branching, and reconnection with the cardinal vein, while decreased leptin signaling via immunoneutralization had an opposing effect on vessel development. We also observed disorganization of major vessels and microvessels of the tail fin and muscle when leptin signaling was decreased. Reduced leptin signaling lowered mRNA expression of <em>cenpk</em>, <em>gpx1</em>, and <em>mmp9</em>, markers for cell proliferation, antioxidation, and extracellular matrix remodeling/cell migration, respectively, in the developing tail, providing insight into three possible mechanisms underlying leptin's promotion of angiogenesis. Together these results illustrate that leptin levels are correlated with embryonic angiogenesis and that leptin coordinates multiple aspects of blood vessel growth and development, showing that leptin is an important morphogen during embryonic development.</p></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140849646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CG9920 is necessary for mitochondrial morphogenesis and individualization during spermatogenesis in Drosophila melanogaster","authors":"Chao Li,&nbsp;Yue Ren,&nbsp;Meng-Yan Chen,&nbsp;Qian Wang,&nbsp;Zhen He,&nbsp;Yu-Feng Wang","doi":"10.1016/j.ydbio.2024.04.008","DOIUrl":"10.1016/j.ydbio.2024.04.008","url":null,"abstract":"<div><p><em>Drosophila melanogaster</em> is an ideal model organism for investigating spermatogenesis due to its powerful genetics, conserved genes and visible morphology of germ cells during sperm production. Our previous work revealed that <em>ocnus</em> (<em>ocn</em>) knockdown resulted in male sterility, and CG9920 was identified as a significantly downregulated protein in fly abdomen after <em>ocn</em> knockdown, suggesting a role of CG9920 in male reproduction. In this study, we found that <em>CG9920</em> was highly expressed in fly testes. <em>CG9920</em> knockdown in fly testes caused male infertility with no mature sperms in seminal vesicles. Immunofluorescence staining showed that depletion of <em>CG9920</em> resulted in scattered spermatid nuclear bundles, fewer elongation cones that did not migrate to the anterior region of the testis, and almost no individualization complexes. Transmission electron microscopy revealed that <em>CG9920</em> knockdown severely disrupted mitochondrial morphogenesis during spermatogenesis. Notably, we found that CG9920 might not directly interact with Ocn, but rather was inhibited by STAT92E, which itself was indirectly affected by Ocn. We propose a possible novel pathway essential for spermatogenesis in <em>D. melanogaster</em>, whereby Ocn indirectly induces <em>CG9920</em> expression, potentially counteracting its inhibition by the JAK-STAT signaling pathway.</p></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140849063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}