Developmental biology最新文献_第10页

Dual role of Xenopus Odf2 in multiciliated cell patterning and differentiation 非洲爪蟾Odf2在多毛细胞模式和分化中的双重作用。

IF 2.5 3区生物学

Developmental biology Pub Date : 2025-01-24 DOI: 10.1016/j.ydbio.2025.01.014

Aude Nommick , Alexandre Chuyen , Raphael Clément , Virginie Thomé , Fabrice Daian , Olivier Rosnet , Fabrice Richard , Nicolas Brouilly , Etienne Loiseau , Camille Boutin , Laurent Kodjabachian

{"title":"Dual role of Xenopus Odf2 in multiciliated cell patterning and differentiation","authors":"Aude Nommick , Alexandre Chuyen , Raphael Clément , Virginie Thomé , Fabrice Daian , Olivier Rosnet , Fabrice Richard , Nicolas Brouilly , Etienne Loiseau , Camille Boutin , Laurent Kodjabachian","doi":"10.1016/j.ydbio.2025.01.014","DOIUrl":"10.1016/j.ydbio.2025.01.014","url":null,"abstract":"<div><div>In developing tissues, the number, position, and differentiation of cells must be coordinately controlled to ensure the emergence of physiological function. The epidermis of the <em>Xenopus</em> embryo contains thousands of uniformly distributed multiciliated cells (MCCs), which grow hundreds of coordinately polarized cilia that beat vigorously to generate superficial water flow. Using this model, we uncovered a dual role for the conserved centriolar component Odf2, in MCC apical organization at the cell level, and in MCC spatial distribution at the tissue level. Like in other species, <em>Xenopus</em> Odf2 localized to the basal foot of basal bodies. Consistently, Odf2 morpholino-mediated knockdown impaired basal foot morphogenesis. Consequently, the rate of microtubule nucleation by Odf2-deficient basal bodies was reduced, leading to cilia disorientation, reduced beating, and ultimately altered flow production across the embryo. Furthermore, we show that Odf2 is required to maintain MCC motility and homotypic repulsion prior to their emergence into the surface layer. Our data suggest that Odf2 promotes MCC spacing via its role in the modulation of cytoplasmic microtubule dynamics. Mathematical simulations confirmed that reduced migration speed alters the spacing order of MCCs. This study provides a striking example of coupling between organizational scales by a unique effector.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"520 ","pages":"Pages 224-238"},"PeriodicalIF":2.5,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143045929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shh signaling directs dorsal ventral patterning in the regenerating X. tropicalis spinal cord Shh信号在再生的热带棘猴脊髓中指导背腹侧模式。

IF 2.5 3区生物学

Developmental biology Pub Date : 2025-01-22 DOI: 10.1016/j.ydbio.2025.01.015

Avery Angell Swearer , Samuel Perkowski , Andrea Wills

{"title":"Shh signaling directs dorsal ventral patterning in the regenerating X. tropicalis spinal cord","authors":"Avery Angell Swearer , Samuel Perkowski , Andrea Wills","doi":"10.1016/j.ydbio.2025.01.015","DOIUrl":"10.1016/j.ydbio.2025.01.015","url":null,"abstract":"<div><div>Tissue development and regeneration rely on the deployment of embryonic signals to drive progenitor activity and thus generate complex cell diversity and organization. One such signal is Sonic Hedgehog (Shh), which establishes the dorsal-ventral (D/V) axis of the spinal cord during embryogenesis. However, the existence of this D/V axis and its dependence on Shh signaling during regeneration varies by species. Here we investigate the function of Shh signaling in patterning the D/V axis during spinal cord regeneration in <em>Xenopus tropicalis</em> tadpoles. We find that neural progenitor markers Msx1/2, Nkx6.1, and Nkx2.2 are confined to dorsal, intermediate and ventral spatial domains, respectively, in both the uninjured and regenerating spinal cord. These domains are altered by perturbation of Shh signaling. Additionally, we find that these D/V domains are more sensitive to Shh perturbation during regeneration than uninjured tissue. The renewed sensitivity of these neural progenitor cells to Shh signals represents a regeneration specific response and raises questions about how responsiveness to developmental patterning cues is regulated in mature and regenerating tissues.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"520 ","pages":"Pages 191-199"},"PeriodicalIF":2.5,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143037356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

At early stages of heart development, the first and second heart fields are a continuum of lateral head mesoderm-derived, cardiogenic cells 在心脏发育的早期阶段，第一和第二心野是由侧头中胚层来源的心源性细胞组成的连续体。

IF 2.5 3区生物学

Developmental biology Pub Date : 2025-01-21 DOI: 10.1016/j.ydbio.2025.01.009

Matthew Wolton , Megan G. Davey , Susanne Dietrich

{"title":"At early stages of heart development, the first and second heart fields are a continuum of lateral head mesoderm-derived, cardiogenic cells","authors":"Matthew Wolton , Megan G. Davey , Susanne Dietrich","doi":"10.1016/j.ydbio.2025.01.009","DOIUrl":"10.1016/j.ydbio.2025.01.009","url":null,"abstract":"<div><div>Pioneering work in the chicken established that the initial development of the heart consists of two stages: the quick assembly of a beating heart, followed by the recruitment of cells from adjacent tissues to deliver the mature in-and outflow tract. Cells to build the primitive heart were dubbed the first heart field (FHF) cells, cells to be recruited later the second heart field (SHF) cells. The current view is that these cells represent distinct, maybe even pre-determined lineages. However, it is still unclear where exactly FHF and SHF are located at different stages of development, and whether there is a sharp boundary or rather an overlap between the two. It is also unclear whether both FHF cells and SHF cells originate from the lateral head mesoderm (LHM), whether the paraxial head mesoderm (PHM) contributes to the SHF, and where the LHM-PHM boundary might be.</div><div>To investigate this problem, we exploited the size, ease of access and exquisite anatomy of the chicken embryo and used traditional strategies as well as newly developed transgenic lines to trace the location of cardiogenic fields and boundaries from the time the first heart-markers are expressed to the time SHF cell recruitment ceases. Our work shows that both FHF and SHF stem from the LHM. We also found that FHF and SHF lack a distinct anatomical boundary. Rather, FHF and SHF are a continuum, and the recruitment of cells into the heart is a chance event depending on morphogenetic movements, the position of cells within the moving tissues, the separation of the somatic and splanchnic LHM, and the separation of the heart from the splanchnic subpharyngeal mesoderm during heart-looping.</div><div>Reconciling our and previous studies we propose that first and second heart field precursors are specified but not determined, thus relying on morphogenetic processes and local environments to realise their cardiogenic potential.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"520 ","pages":"Pages 200-223"},"PeriodicalIF":2.5,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Naturally occurring, rostrally conjoining chicken twins attempt to make a forebrain 自然发生的，雌性连体鸡双胞胎试图制造一个前脑。

IF 2.5 3区生物学

Developmental biology Pub Date : 2025-01-21 DOI: 10.1016/j.ydbio.2025.01.013

Frank R. Schubert , Susanne Dietrich

{"title":"Naturally occurring, rostrally conjoining chicken twins attempt to make a forebrain","authors":"Frank R. Schubert , Susanne Dietrich","doi":"10.1016/j.ydbio.2025.01.013","DOIUrl":"10.1016/j.ydbio.2025.01.013","url":null,"abstract":"<div><div>Conjoined twinning is a special case of monozygotic, monoamniotic twinning. Human conjoined twinning, and vertebrate conjoined twinning in general, is a very rare phenomenon. It has been suggested that the risk of conjoined twinning increases with some medication and upon assisted reproduction. Survival rates are low.</div><div>When conjoined twins occur in the chicken, they most often present with fused heads, anatomically unrecognisable brains and two normal bodies. Recent studies suggested that forebrain, midbrain and rostral hindbrain identities are established in the early epiblast before neural induction and independent from caudal hindbrain and spinal cord identities. Therefore, it is unclear whether in conjoined twins, the aberrant brain anatomy is a result of the rostral fusion, or whether the brains failed to develop in the first place.</div><div>Here, we collected conjoined twins as they spontaneously appeared in eggs incubated for stages HH4 (late primitive steak stage) to HH13 (early pharyngula). The twinned embryos and stage-matched normal embryos were analysed for the expression of the rostral epiblast and forebrain-midbrain marker <em>Otx2</em> and the ventral forebrain marker <em>Six3</em>. We found normal anatomy and marker gene expression that lasted up to stage HH9. By HH12-13, the brain anatomy had deteriorated, but marker genes remained expressed. This suggests that the fusing embryos attempted to generate a brain including the forebrain.</div><div>Besides addressing forebrain development, our work for the first time provides a time frame to study the mechanisms underlying the interaction and fusion of conjoined twins, which will pave the way to a better understanding and management of risk factors in humans.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"520 ","pages":"Pages 171-179"},"PeriodicalIF":2.5,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Loss of KAT6B causes premature ossification and promotes osteoblast differentiation during development KAT6B缺失会导致过早骨化，并在发育过程中促进成骨细胞分化。

IF 2.5 3区生物学

Developmental biology Pub Date : 2025-01-19 DOI: 10.1016/j.ydbio.2025.01.012

Maria I. Bergamasco , Jacqueline M. Ogier , Alexandra L. Garnham , Lachlan Whitehead , Kelly Rogers , Gordon K. Smyth , Rachel A. Burt , Anne K. Voss , Tim Thomas

{"title":"Loss of KAT6B causes premature ossification and promotes osteoblast differentiation during development","authors":"Maria I. Bergamasco , Jacqueline M. Ogier , Alexandra L. Garnham , Lachlan Whitehead , Kelly Rogers , Gordon K. Smyth , Rachel A. Burt , Anne K. Voss , Tim Thomas","doi":"10.1016/j.ydbio.2025.01.012","DOIUrl":"10.1016/j.ydbio.2025.01.012","url":null,"abstract":"<div><div>The MYST family histone acetyltransferase gene, KAT6B (MYST4, MORF, QKF) is mutated in two distinct human congenital disorders characterised by intellectual disability, facial dysmorphogenesis and skeletal abnormalities; the Say-Barber-Biesecker-Young-Simpson variant of Ohdo syndrome and Genitopatellar syndrome. Despite its requirement in normal skeletal development, the cellular and transcriptional effects of KAT6B in skeletogenesis have not been thoroughly studied. Here, we show that germline deletion of the <em>Kat6b</em> gene in mice causes premature ossification <em>in vivo</em>, resulting in shortened craniofacial elements and increased bone density, as well as shortened tibias with an expanded pre-hypertrophic layer, as compared to wild type controls. Mechanistically, we show that the loss of KAT6B in mesenchymal progenitor cells promotes transition towards an osteoblast-progenitor state with upregulation of gene targets of RUNX2, a master regulator of osteoblast development and concomitant downregulation of SOX9, a critical gene in chondrocyte development. Moreover, we find that compound heterozygosity at <em>Kat6b</em> and <em>Runx2</em> loci partially rescues the reduction in ossification of <em>Runx2</em> heterozygous, but not homozygous mice, suggesting that KAT6B may limit the action of RUNX2, possibly through a role in maintaining progenitors in an undifferentiated state. Moreover, our results show that KAT6B has essential roles in regulating the expression of a large number of genes involved in skeletogenesis and bone development.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"520 ","pages":"Pages 141-154"},"PeriodicalIF":2.5,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Outside Back Cover - Graphical abstract TOC/TOC in double column/Cover image legend if applicable, Bar code, Abstracting and Indexing information 封底外-图形摘要TOC/双栏TOC/封面图例（如适用），条形码，摘要和索引信息

IF 2.5 3区生物学

Developmental biology Pub Date : 2025-01-18 DOI: 10.1016/S0012-1606(25)00019-3

引用次数: 0

The role of serendipity in our investigation of embryo implantation 偶然性在胚胎着床研究中的作用。

IF 2.5 3区生物学

Developmental biology Pub Date : 2025-01-16 DOI: 10.1016/j.ydbio.2025.01.010

Ann E. Sutherland

{"title":"The role of serendipity in our investigation of embryo implantation","authors":"Ann E. Sutherland","doi":"10.1016/j.ydbio.2025.01.010","DOIUrl":"10.1016/j.ydbio.2025.01.010","url":null,"abstract":"<div><div>Serendipity plays a huge role in science, and having a prepared mind that can seize upon a chance observation or occurrence can drive a project forward. This happened in my lab with a project centered on the regulation of trophoblast cell behavior at implantation. We discovered that amino acids regulate the onset of trophoblast motility through the activation of the kinase complex mTORC1, and that this acts as a checkpoint to trophoblast differentiation. This finding not only broadened our understanding of the mechanisms underlying embryo implantation, but also provided new ways of thinking about the regulation of diapause, a state of suspended embryonic development that occurs in many species. I should say that we <u>re-discovered</u> the fact that amino acids regulate the onset of trophoblast motility, as reading the literature showed us that others had made this same observation some 30 years previously and we were fortuitously able to build upon those findings. This project confirmed to me how valuable it is to read the literature widely, both historical papers and those in fields outside one's area of research, and to go to seminars on topics outside one's area.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"520 ","pages":"Pages 135-140"},"PeriodicalIF":2.5,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mammalian lactation as a framework for teaching development, physiology, and cell biology for social change 哺乳动物哺乳作为教学发展的框架，生理学和细胞生物学的社会变革。

IF 2.5 3区生物学

Developmental biology Pub Date : 2025-01-16 DOI: 10.1016/j.ydbio.2025.01.011

Melissa E. Marks, Emma J. Coddington Brown

{"title":"Mammalian lactation as a framework for teaching development, physiology, and cell biology for social change","authors":"Melissa E. Marks, Emma J. Coddington Brown","doi":"10.1016/j.ydbio.2025.01.011","DOIUrl":"10.1016/j.ydbio.2025.01.011","url":null,"abstract":"<div><div>Mammalian lactation is a dynamic process that develops throughout the lifespan of an organism. Here we present a framework for a third semester core course in biology that centers course content on lactation allowing examination of the developmental process as a dynamic whole-body experience involving changes occurring at the molecular, cellular, and organ levels of organization. Inequitable economic, socio- and geopolitical systems structure social determinants of health, affecting rates of breastfeeding in human populations. By integrating content exploring the ways social and biological systems impact breastfeeding rates in human populations, students develop abilities to understand the relationship between science and society throughout the course, a critical core competency for engaging in social change. Importantly, they interrogate social systems while simultaneously learning about many canonical biological processes including how natural selection and constraint have shaped the anatomy, physiology, cell biology, and biochemistry of lactation, how proteins, lipids, and carbohydrates are synthesized, processed, and exported through the endomembrane system in eukaryotes, and how neuronal and hormonal feedback mechanisms regulate milk synthesis and secretion. The course is structured using a flipped-classroom design emphasizing revision and student-self assessment that supports development of biological knowledge, social responsibility, and metacognitive skills. Because mammalian lactation includes fascinating, nuanced, and complex components that cross interdisciplinary boundaries, it provides a wealth of opportunities for faculty to teach developmental biology for social change.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"520 ","pages":"Pages 180-190"},"PeriodicalIF":2.5,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interplay of canonical and LIMK mediated non-canonical BMP signaling is essential for regulating differential thickness and invagination during chick forebrain roof plate morphogenesis 规范和LIMK介导的非规范BMP信号的相互作用对调节鸡前脑顶板形态发生过程中的差异厚度和内陷至关重要。

IF 2.5 3区生物学

Developmental biology Pub Date : 2025-01-15 DOI: 10.1016/j.ydbio.2025.01.008

Mohd Ali Abbas Zaidi , Sweta Kushwaha , Niveda Udaykumar , Pallavi Dethe , Meenu Sachdeva , Jonaki Sen

{"title":"Interplay of canonical and LIMK mediated non-canonical BMP signaling is essential for regulating differential thickness and invagination during chick forebrain roof plate morphogenesis","authors":"Mohd Ali Abbas Zaidi , Sweta Kushwaha , Niveda Udaykumar , Pallavi Dethe , Meenu Sachdeva , Jonaki Sen","doi":"10.1016/j.ydbio.2025.01.008","DOIUrl":"10.1016/j.ydbio.2025.01.008","url":null,"abstract":"<div><div>Telencephalic hemisphere formation is a complex and precisely timed process, which begins in the chick forebrain with an invagination in the middle of the roof plate. However, the factor(s) that determine the position/site of invagination in the roof plate remain to be elucidated. In this study, we have demonstrated that as development proceeds, a region of lower thickness appears in the middle of the roof plate, which marks the position where the invagination begins. Our investigations have implicated an interplay between the canonical (pSMAD 1/5/9 dependent) and the non-canonical (LIMK dependent) arms of BMP signaling in regulating this process. We have demonstrated that LIMK dependent non-canonical BMP signaling induces high levels of phosphorylated Cofilin (pCofilin) in the middle of the roof plate, which in turn alters Actin cytoskeleton dynamics, resulting in this region being thinner than the lateral regions. This study has provided the first mechanistic insight into how forebrain roof plate invagination begins and has thrown light on the role played by BMP signaling in this process.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"520 ","pages":"Pages 125-134"},"PeriodicalIF":2.5,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of lens-regulated genes driving anterior eye development 晶状体调控基因驱动前眼发育的鉴定。

IF 2.5 3区生物学

Developmental biology Pub Date : 2025-01-13 DOI: 10.1016/j.ydbio.2025.01.004

Verónica Murcia-Belmonte , Yanlin Liu , Sadia Shamsi , Sophie Shaw , Elaina Collie-Duguid , Eloisa Herrera , J. Martin Collinson , Neil Vargesson , Lynda Erskine

{"title":"Identification of lens-regulated genes driving anterior eye development","authors":"Verónica Murcia-Belmonte , Yanlin Liu , Sadia Shamsi , Sophie Shaw , Elaina Collie-Duguid , Eloisa Herrera , J. Martin Collinson , Neil Vargesson , Lynda Erskine","doi":"10.1016/j.ydbio.2025.01.004","DOIUrl":"10.1016/j.ydbio.2025.01.004","url":null,"abstract":"<div><div>Signals from the lens regulate multiple aspects of eye development, including establishment of eye size, patterning of the presumptive iris and ciliary body in the anterior optic cup and migration and differentiation of neural crest cells. To advance understanding of the molecular mechanism by which the lens regulates eye development, we performed transcriptome profiling of embryonic chicken retinas after lens removal. Genes associated with nervous system development were upregulated in lens-removed eyes, but the presumptive ciliary body and iris region did not adopt a neural retina identity following lens removal. Lens-regulated genes implicated in periocular mesenchyme, cornea and anterior optic cup development were identified, including factors not previously implicated in eye development. Unexpectedly, transcriptomic differences were identified in retinas from male versus female chicken embryos, suggesting sexual dimorphism from early stages. In situ hybridisation of embryonic chicken eyes and analyses of datasets from embryonic mouse and adult human eyes confirmed expression of candidate genes, including multiple WNT genes, in tissues important for anterior eye development and function. Remarkably, pharmacological activation of canonical WNT signalling restored eye development and size in the absence of the lens. These analyses have identified candidate genes and biological pathways involved in eye development, providing avenues for new research in this area.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"520 ","pages":"Pages 91-107"},"PeriodicalIF":2.5,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0