Developmental biology最新文献

{"title":"Resilience of the replacing dentition in adult reptiles","authors":"Joaquin I. Henriquez,&nbsp;Joy M. Richman","doi":"10.1016/j.ydbio.2024.07.013","DOIUrl":"10.1016/j.ydbio.2024.07.013","url":null,"abstract":"<div><p>The dentition is critical to animal survival and teeth are present in modern vertebrates including teleost fish, sharks, amphibians, mammals and reptiles. The developmental processes that give rise to teeth are not just preserved through evolution but also share high level of similarity with the embryogenesis of other ectodermal organs. In this review we go beyond the embryonic phase of tooth development to life-long tooth replacement. We will address the origins of successional teeth, the location of putative tissue-resident stem cells, how de novo tooth formation continues throughout life and how teeth are shed in a spatially and temporally controlled manner. We review the evidence that the dental epithelium, which is the earliest recognizable dental structure in the reptilian dentition, serves as a putative niche for tissue-resident epithelial stem cells and recent molecular findings from transcriptomics carried out in reptilian dentitions. We discuss how odontoclasts resorb the primary tooth allowing eruption of the successional tooth. The reptiles, particularly lizards, are emerging as some of the most accessible animals to study tooth replacement which has relevance to evolution of the dentition and human dental disorders.</p></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"516 ","pages":"Pages 71-81"},"PeriodicalIF":2.5,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0012160624001933/pdfft?md5=47b7930801fde318b9cdd6ff4efb01b8&pid=1-s2.0-S0012160624001933-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141765756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emergence and properties of adult mammalian epidermal stem cells","authors":"Purna Gadre,&nbsp;Pavlina Markova,&nbsp;Mirsana Ebrahimkutty,&nbsp;Yidan Jiang,&nbsp;Francisco M. Bouzada,&nbsp;Fiona M. Watt","doi":"10.1016/j.ydbio.2024.07.014","DOIUrl":"10.1016/j.ydbio.2024.07.014","url":null,"abstract":"<div><p>In this review we discuss how the mammalian interfollicular epidermis forms during development, maintains homeostasis, and is repaired following wounding. Recent studies have provided new insights into the relationship between the stem cell compartment and the differentiating cell layers; the ability of differentiated cells to dedifferentiate into stem cells; and the epigenetic memory of epidermal cells following wounding.</p></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"515 ","pages":"Pages 129-138"},"PeriodicalIF":2.5,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0012160624001945/pdfft?md5=1bab65773b8d893f57f0eaa92a5bf4a5&pid=1-s2.0-S0012160624001945-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141765755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of gestational hypothyroidism on mouse brain development: Gabaergic systems and oxidative stress","authors":"Edênia da Cunha Menezes ,&nbsp;Fabiula Francisca de Abreu ,&nbsp;Jada B. Davis ,&nbsp;Sara V. Maurer ,&nbsp;Venezia C. Roshko ,&nbsp;Angela Richardson ,&nbsp;Jonathan Dowell ,&nbsp;Sarah N. Cassella ,&nbsp;Hanna E. Stevens","doi":"10.1016/j.ydbio.2024.07.010","DOIUrl":"10.1016/j.ydbio.2024.07.010","url":null,"abstract":"<div><p>Hormonal imbalance during pregnancy is a risk factor for neuropsychiatric impairment in the offspring. It has been suggested that hypothyroidism leads to dysfunction of cortical GABAergic interneurons and inhibitory system development that in turn underlies impairment of the central nervous system. Here we investigated how gestational hypothyroidism affected offspring GABAergic system development as well as redox regulation parameters, because of previous links identified between the two. Experimental Gestational Hypothyroidism (EGH) was induced in CD-1 mice with 0.02% methimazole (MMI) in drinking water from embryonic day 9 (E9) until tissue collection at embryonic day 14 (E14) or E18. We examined GABAergic cell distribution and inhibitory system development gene expression as well as redox relevant gene expression and direct measures across all embryos regardless of sex. Intrauterine restriction of maternal thyroid hormones significantly impacted both of these outcomes in brain, as well as altering redox regulation in the placenta. GAD67+ neuronal migration was reduced, accompanied by a disruption in gene expression influencing GABAergic cell migration and cortical inhibitory neural system development. EGH also altered embryonic brain gene expression of <em>Gpx1, Nfe2l2, Cat</em> levels in the dorsal E14 brains. Additionally, EGH resulted in elevated TBARS, <em>Gpx1</em> and <em>Nfe2l2</em> in the ventral E18 brains. Furthermore, EGH downregulated placental <em>Gpx1</em> gene expression at E14 and increased protein oxidation at E18. These findings support the hypothesis that sufficient maternal thyroid hormone supply to the fetus influences central nervous system development, including processes of GABAergic system development and redox equilibrium.</p></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"515 ","pages":"Pages 112-120"},"PeriodicalIF":2.5,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141757705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maintenance of germline stem cell homeostasis despite severe nuclear distortion","authors":"Isabella E. Perales,&nbsp;Samuel D. Jones,&nbsp;Tingting Duan ,&nbsp;Pamela K. Geyer","doi":"10.1016/j.ydbio.2024.07.009","DOIUrl":"10.1016/j.ydbio.2024.07.009","url":null,"abstract":"<div><p>Stem cell loss in aging and disease is associated with nuclear deformation. Yet, how nuclear shape influences stem cell homeostasis is poorly understood. We investigated this connection using Drosophila germline stem cells, as survival of these stem cells is compromised by dysfunction of the nuclear lamina, the extensive protein network that lines the inner nuclear membrane and gives shape to the nucleus. To induce nuclear distortion in germline stem cells, we used the GAL4-UAS system to increase expression of the permanently farnesylated nuclear lamina protein, Kugelkern, a rate limiting factor for nuclear growth. We show that elevated Kugelkern levels cause severe nuclear distortion in germline stem cells, including extensive thickening and lobulation of the nuclear envelope and nuclear lamina, as well as alteration of internal nuclear compartments. Despite these changes, germline stem cell number, proliferation, and female fertility are preserved, even as females age. Collectively, these data demonstrate that disruption of nuclear architecture does not cause a failure of germline stem cell survival or homeostasis, revealing that nuclear deformation does not invariably promote stem cell loss.</p></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"515 ","pages":"Pages 139-150"},"PeriodicalIF":2.5,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141747688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progressive modifications during evolution involving epigenetic changes have determined loss of regeneration mainly in terrestrial animals: A hypothesis","authors":"Lorenzo Alibardi","doi":"10.1016/j.ydbio.2024.07.007","DOIUrl":"10.1016/j.ydbio.2024.07.007","url":null,"abstract":"<div><p>In order to address a biological explanation for the different regenerative abilities present among animals, a new evolutionary speculation is presented. It is hypothesized that epigenetic mechanisms have lowered or erased regeneration during the evolution of terrestrial invertebrates and vertebrates. The hypothesis indicates that a broad regeneration can only occur in marine or freshwater conditions, and that life on land does not allow for high regeneration. This is due to the physical, chemical and microbial conditions present in the terrestrial environment with respect to those of the aquatic environment. The present speculation provides examples of hypothetic evolutionary animal lineages that colonized the land, such as parasitic annelids, terrestrial mollusks, arthropods and amniotes. These are the animals where regeneration is limited or absent and their injuries are only repaired through limited healing or scarring. It is submitted that this loss derived from changes in the developmental gene pathways sustaining regeneration in the aquatic environment but that cannot be expressed on land. Once regeneration was erased in terrestrial species, re-adaptation to freshwater niches could not reactivate the previously altered gene pathways that determined regeneration. Therefore a broad regeneration was no longer possible or became limited and heteromorphic in the derived, extant animals. Only in few cases extensive healing abilities or regengrow<em>,</em> a healing process where regeneration overlaps with somatic growth, have evolved among arthropods and amniotes. The present paper is an extension of previous speculations trying to explain in biological terms the different regenerative abilities present among metazoans.</p></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"515 ","pages":"Pages 169-177"},"PeriodicalIF":2.5,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141726957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth patterns of caudal fin rays are informed by both external signals from the regenerating organ and remembered identity autonomous to the local tissue","authors":"Melody Autumn,&nbsp;Yinan Hu,&nbsp;Jenny Zeng,&nbsp;Sarah K. McMenamin","doi":"10.1016/j.ydbio.2024.07.008","DOIUrl":"10.1016/j.ydbio.2024.07.008","url":null,"abstract":"<div><p>Regenerating tissues must remember or interpret their spatial position, using this information to restore original size and patterning. The external skeleton of the zebrafish caudal fin is composed of 18 rays; after any portion of the fin is amputated, position-dependent regenerative growth restores each ray to its original length. We tested for transcriptional differences during regeneration of proximal versus distal tissues and identified 489 genes that differed in proximodistal expression. Thyroid hormone directs multiple aspects of ray patterning along the proximodistal axis, and we identified 364 transcripts showing a proximodistal expression pattern that was dependent on thyroid hormone context. To test what aspects of ray positional identity are directed by extrinsic environental cues versus remembered identity autonomous to the tissue, we transplanted distal portions of rays to proximal environments and evaluated regeneration within the new location. Native regenerating proximal tissue showed robust expression of <em>scpp7</em>, a transcript with thyroid-regulated proximal enrichment; in contrast, regenerating rays originating from transplanted distal tissue showed reduced (distal-like) expression during outgrowth. These distal-to-proximal transplants regenerated far beyond the length of the graft itself, indicating that cues from the proximal environment promoted additional growth. Nonetheless, these transplants initiated regeneration at a much slower rate compared to controls, suggesting memory of distal identity was retained by the transplanted tissue. This early growth retardation caused rays that originated from transplants to grow noticeably shorter than neighboring native rays. While several aspects of fin ray morphology (bifurcation, segment length) were found to be determined by the environment, we found that both regeneration speed and ray length are remembered autonomously by tissues, and that persist through multiple rounds of amputation and regeneration.</p></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"515 ","pages":"Pages 121-128"},"PeriodicalIF":2.5,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141726931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CPLANE protein INTU regulates growth and patterning of the mouse lungs through cilia-dependent Hh signaling","authors":"Huiqing Zeng ,&nbsp;Shimaa Ali ,&nbsp;Aswathy Sebastian ,&nbsp;Adriana Sophia Ramos-Medero ,&nbsp;Istvan Albert ,&nbsp;Charlotte Dean ,&nbsp;Aimin Liu","doi":"10.1016/j.ydbio.2024.07.006","DOIUrl":"10.1016/j.ydbio.2024.07.006","url":null,"abstract":"<div><p>Congenital lung malformations are fatal at birth in their severe forms. Prevention and early intervention of these birth defects require a comprehensive understanding of the molecular mechanisms of lung development. We find that the loss of <em>inturned</em> (<em>Intu</em>), a cilia and planar polarity effector gene, severely disrupts growth and branching morphogenesis of the mouse embryonic lungs. Consistent with our previous results indicating an important role for <em>Intu</em> in ciliogenesis and hedgehog (Hh) signaling, we find greatly reduced number of primary cilia in both the epithelial and mesenchymal tissues of the lungs. We also find significantly reduced expression of <em>Gli1</em> and <em>Ptch1</em>, direct targets of Hh signaling, suggesting disruption of cilia-dependent Hh signaling in <em>Intu</em> mutant lungs. An agonist of the Hh pathway activator, smoothened, increases Hh target gene expression and tubulogenesis in explanted wild type, but not <em>Intu</em> mutant, lungs, suggesting impaired Hh signaling response underlying lung morphogenetic defects in <em>Intu</em> mutants. Furthermore, removing both <em>Gli2</em> and <em>Intu</em> completely abolishes branching morphogenesis of the lung, strongly supporting a mechanism by which <em>Intu</em> regulates lung growth and patterning through cilia-dependent Hh signaling. Moreover, a transcriptomics analysis identifies around 200 differentially expressed genes (DEGs) in <em>Intu</em> mutant lungs, including known Hh target genes <em>Gli1, Ptch1/2</em> and <em>Hhip</em>. Genes involved in muscle differentiation and function are highly enriched among the DEGs, consistent with an important role of Hh signaling in airway smooth muscle differentiation. In addition, we find that the difference in gene expression between the left and right lungs diminishes in <em>Intu</em> mutants, suggesting an important role of <em>Intu</em> in asymmetrical growth and patterning of the mouse lungs.</p></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"515 ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141726930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tlx3 mediates neuronal differentiation and proper condensation of the developing trigeminal ganglion","authors":"Hugo A. Urrutia,&nbsp;Jan Stundl,&nbsp;Marianne E. Bronner","doi":"10.1016/j.ydbio.2024.07.005","DOIUrl":"10.1016/j.ydbio.2024.07.005","url":null,"abstract":"<div><p>The trigeminal ganglion, the largest of the vertebrate cranial ganglia, is comprised of sensory neurons that relay sensations of pain, touch, and temperature to the brain. These neurons are derived from two embryonic cell types, the neural crest and ectodermal placodes, whose interactions are critical for proper ganglion formation. While the T-cell leukemia homeobox 3 (<em>Tlx3</em>) gene is known to be expressed in placodally-derived sensory neurons and necessary for their differentiation, little was known about <em>Tlx3</em> expression and/or function in the neural crest-derived component of the developing trigeminal ganglion. By combining lineage labeling with <em>in situ</em> hybridization in the chick embryo, we show that neural crest-derived cells that contribute to the cranial trigeminal ganglion express <em>Tlx3</em> at a time point that coincides with the onset of ganglion condensation. Importantly, loss of <em>Tlx3</em> function <em>in vivo</em> diminishes the overall size and abundance of neurons within the trigeminal ganglion. Conversely, ectopic expression of <em>Tlx3</em> in migrating cranial neural crest results in their premature neuronal differentiation. Taken together, our results demonstrate a critical role for <em>Tlx3</em> in neural crest-derived cells during chick trigeminal gangliogenesis.</p></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"515 ","pages":"Pages 79-91"},"PeriodicalIF":2.5,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141632955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"“When does human life begin?” teaching human embryology in the context of the American abortion debate","authors":"Scott F. Gilbert","doi":"10.1016/j.ydbio.2024.07.003","DOIUrl":"10.1016/j.ydbio.2024.07.003","url":null,"abstract":"<div><p>The Dobbs decision of the United States Supreme Court and the actions of several state legislatures have made it risky, if not outright dangerous, to teach factual material concerning human embryology. At some state universities, for instance, if a professor's lecture is felt to teach or discuss abortion (as it might when teaching about tubal pregnancies, hydatidiform moles, or eneuploidy), that instructor risks imprisonment for up to 14 years (Gyori, 2023). Some states' new censorship rules have thus caused professors to drop modules on abortion from numerous science and humanities courses. In most states, instructors can still teach about human embryonic development and not risk putting their careers or livelihoods in jeopardy. However, even in many of these institutions, students can bring a professor to a disciplinary hearing by claiming that the instructor failed to provide ample trigger warnings on such issues. This essay attempts to provide some strategies wherein human embryology and the ethical issues surrounding it might be taught and students may be given resources to counter unscientific falsehoods about fertilization and human development. This essay provides evidence for teaching the following propositions.</p><p>Mis-information about human biology and medicine is rampant on the internet, and there are skills that can be taught to students that will help them determine which sites should trusted. This is a skill that needs to be taught as part of science courses.</p></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"515 ","pages":"Pages 102-111"},"PeriodicalIF":2.5,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141616073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contribute to special issues in developmental biology","authors":"Sally A. Moody","doi":"10.1016/j.ydbio.2024.07.004","DOIUrl":"10.1016/j.ydbio.2024.07.004","url":null,"abstract":"","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"515 ","pages":"Page 59"},"PeriodicalIF":2.5,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141619542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}