Developmental biology最新文献_第9页

Disruption of spindle orientation and protein localization during asymmetric cleavage by pharmacological inhibition of serotonin signaling 药物抑制血清素信号传导对不对称切割过程中纺锤体取向和蛋白质定位的影响。

IF 2.5 3区生物学

Developmental biology Pub Date : 2025-06-29 DOI: 10.1016/j.ydbio.2025.06.025

Ayaki Nakamoto , Lisa M. Nagy

{"title":"Disruption of spindle orientation and protein localization during asymmetric cleavage by pharmacological inhibition of serotonin signaling","authors":"Ayaki Nakamoto , Lisa M. Nagy","doi":"10.1016/j.ydbio.2025.06.025","DOIUrl":"10.1016/j.ydbio.2025.06.025","url":null,"abstract":"<div><div>Cell polarity directs the orientation and size of asymmetric cell division and the segregation of cell fate determinants, processes fundamental to development in all multicellular organisms. During asymmetric cleavage, the mitotic spindle aligns with a specified polarity of the mother cell, and cell fate determinants are distributed asymmetrically along the division axis. Here, we report that pharmacological inhibition of serotonin signaling during the 4-to-8-cell division in early embryos of the mud snail <em>Ilyanassa obsoleta</em> (currently known as <em>Tritia obsoleta</em>) disrupts the typical unequal division pattern. The oblique axis of division common to spirally cleaving molluscan embryos is altered, and the position of the mitotic spindle is randomized in these treatments. Mother cells generate abnormally large, atypically positioned daughter cells. We also find that, in normal embryos, proteins recognized by phosphorylated PKC and Bazooka/PAR-3 antibodies typically co-localize with the spindle apparatus to the apical cortex of each mother cell. These antigens subsequently segregate to the smaller of the two daughter cells. In embryos treated with the serotonin-receptor antagonist, the localization of these asymmetrically segregating proteins is randomized, and their localization is independent of spindle position. These results suggest that serotonin signaling coordinates spindle orientation, cortical polarity, and cell size in early asymmetric cleavages.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"526 ","pages":"Pages 26-37"},"PeriodicalIF":2.5,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144539408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Somites use membrane potential to synchronize growth and segmentation 有的利用膜电位来同步生长和分割。

IF 2.1 3区生物学

Developmental biology Pub Date : 2025-06-26 DOI: 10.1016/j.ydbio.2025.06.015

Raisa Bailon-Zambrano

引用次数: 0

Born in the heat: why rising temperatures matter for sea turtles 在高温中出生：为什么气温上升对海龟很重要。

IF 2.1 3区生物学

Developmental biology Pub Date : 2025-06-26 DOI: 10.1016/j.ydbio.2025.06.014

Caroline Halmi

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Wintry temps won’t stop the clock 寒冷的气温不会让时钟停止转动。

IF 2.1 3区生物学

Developmental biology Pub Date : 2025-06-26 DOI: 10.1016/j.ydbio.2025.06.016

Cindy Ow

引用次数: 0

To the right, to the right – Chick it out! 往右转，往右转-滚出去！

IF 2.1 3区生物学

Developmental biology Pub Date : 2025-06-26 DOI: 10.1016/j.ydbio.2025.06.017

Samantha Z. Fernandes

引用次数: 0

Undergraduate scientists jump into the deep end of zebrafish sensory support cell plasticity 本科科学家跳入斑马鱼感觉支持细胞可塑性的深端。

IF 2.1 3区生物学

Developmental biology Pub Date : 2025-06-26 DOI: 10.1016/j.ydbio.2025.06.018

Violet Sorrentino

引用次数: 0

Investigating the dynamic signaling pathways involved in the specialization and differentiation of epiblast cells in early human embryos 研究早期人类胚胎外胚层细胞特化和分化的动态信号通路。

IF 2.5 3区生物学

Developmental biology Pub Date : 2025-06-26 DOI: 10.1016/j.ydbio.2025.05.014

Yong Wang , Lijing Wu , Meifeng Zheng , Jianhua Zhu , Lujuan Rong , Kaimin Yang , Xinyue Hu , Lifeng Xiang , Kui Duan

{"title":"Investigating the dynamic signaling pathways involved in the specialization and differentiation of epiblast cells in early human embryos","authors":"Yong Wang , Lijing Wu , Meifeng Zheng , Jianhua Zhu , Lujuan Rong , Kaimin Yang , Xinyue Hu , Lifeng Xiang , Kui Duan","doi":"10.1016/j.ydbio.2025.05.014","DOIUrl":"10.1016/j.ydbio.2025.05.014","url":null,"abstract":"<div><div>The human embryonic epiblast is essential for early development, ultimately giving rise to all somatic and germ cell lineages. Despite advances in understanding embryogenesis, the mechanisms regulating intercellular communication during epiblast specification remain incompletely understood. Here, we analyzed single-cell RNA sequencing data spanning pre-implantation, post-implantation, pre-gastrulation, and early-gastrulation stages of human embryos to investigate how signals from extra-embryonic tissues influence epiblast (EPI) development. Our data-driven analysis indicates that multiple signaling pathways, including BMP, WNT, FGF, LIF, and TGF<span><math><mi>β</mi></math></span>, may be involved in the first and second lineage separations, amniotic epithelial (AME) cell development, and primitive streak (PS) formation. We further propose that the inner cell mass (ICM) and EPI could function as signaling hubs, coordinating critical intercellular communication events. Based on RNA expression patterns, extra-embryonic tissues such as the hypoblast, trophoblast (TrB), and extra-embryonic mesoderm (ExM) appear to secrete key signals (BMP4, SELL, WNTs, and PTN) that potentially regulate EPI cell polarization, EPI-AME transdifferentiation, and PS development. Notably, BMP4 expression may follow a dynamic pattern, transitioning from early implantation visceral/parietal endoderm (VE/YE) cells to pre-gastrulation ExM cells and ultimately to CS7 advanced mesoderm cells. Overall, these findings provide a comprehensive overview of putative signaling events mediated by extra-embryonic tissues and underscore their potential roles in orchestrating epiblast development and early lineage decisions in the human embryo, while highlighting the need for further protein-level and functional validation.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"526 ","pages":"Pages 98-110"},"PeriodicalIF":2.5,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144526788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

There is no replacement for animal models in medical research 在医学研究中，动物模型是不可替代的。

IF 2.1 3区生物学

Developmental biology Pub Date : 2025-06-25 DOI: 10.1016/j.ydbio.2025.06.023

Carole LaBonne

引用次数: 0

Engineering development: From the repressilator and toggle switch to synthetic developmental biology 工程发展：从减压器和开关到合成发育生物学。

IF 2.5 3区生物学

Developmental biology Pub Date : 2025-06-25 DOI: 10.1016/j.ydbio.2025.06.021

Margaret S. Saha , Kate B.R. Carline , Madeline W. Eibner-Gebhardt , Grace E. Hussey , Marcus O. Royster

{"title":"Engineering development: From the repressilator and toggle switch to synthetic developmental biology","authors":"Margaret S. Saha , Kate B.R. Carline , Madeline W. Eibner-Gebhardt , Grace E. Hussey , Marcus O. Royster","doi":"10.1016/j.ydbio.2025.06.021","DOIUrl":"10.1016/j.ydbio.2025.06.021","url":null,"abstract":"<div><div>The publication of two landmark papers in the year 2000 demonstrated that scientists could rationally engineer genetic circuits to perform desired biological processes commonly found in nature. Elowitz and Leibler constructed a repressilator based on a negative feedback loop that showed oscillatory behavior, and the Collins lab engineered a toggle switch that was able to switch between and maintain stable states in response to external stimuli. Although built upon a long history of conceptual and technical advances in the field, both studies were nevertheless instrumental in transforming the way investigators studied complex biological processes. Rather than using a top-down approach to dissect and analyze individual components of biological processes, both research teams employed a bottom-up approach, creating genetic devices that reconstructed fundamental cellular processes—in essence, building to understand. As described in this review, this bottom-up approach was particularly transformative in the field of developmental biology where the “build to understand” approach yielded important insights into long-standing problems in developmental biology: the mechanisms underlying promiscuous, combinatorial signaling; how cells acquire a stable fate; and the emergence of complex patterns. Additionally, these papers laid the foundation for an array of technical advances that spawned the field of synthetic developmental biology and its fruitful applications to tissue engineering and regenerative medicine.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"526 ","pages":"Pages 82-97"},"PeriodicalIF":2.5,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Roles played by Enhancer of split transcription factors in Drosophila R7 photoreceptor specification 分裂转录因子增强子在果蝇R7光感受器规范中的作用

IF 2.5 3区生物学

Developmental biology Pub Date : 2025-06-25 DOI: 10.1016/j.ydbio.2025.06.024

Ronald A. Arias, Yannis Emmanuel Mavromatakis, Andrew Tomlinson

{"title":"Roles played by Enhancer of split transcription factors in Drosophila R7 photoreceptor specification","authors":"Ronald A. Arias, Yannis Emmanuel Mavromatakis, Andrew Tomlinson","doi":"10.1016/j.ydbio.2025.06.024","DOIUrl":"10.1016/j.ydbio.2025.06.024","url":null,"abstract":"<div><div>When a cell receives multiple developmental signals simultaneously, the intracellular transduction pathways triggered by those signals are coincidentally active. How then, do the cells decode the information contained within those multiple active pathways to derive a precise developmental directive? The specification of the Drosophila R7 photoreceptor is a classic model system for investigating such questions. The R7 fate is specified by the combined actions of the Notch (N) and receptor tyrosine kinase (RTK) signaling pathways. The two pathways cross-communicate in an integrative mechanism and also supply information independently of each other. Collectively, this information is summed to provide an unambiguous directive for the R7 fate. Our goal is to understand these mechanisms. Here, we examine how N activity represses transcription of the <em>phyllopod</em> gene in the process of information integration with the RTK pathway, and how it represses expression of the <em>seven-up</em> gene in an independent mechanism needed for R7 fate. We describe how N activity achieves these transcriptional repressions and identify Enhancer of Split transcription factors as the mediators of its functions.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"526 ","pages":"Pages 38-51"},"PeriodicalIF":2.5,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0