Developmental biology最新文献

{"title":"Reproductive adaptation of Astyanax mexicanus under nutrient limitation","authors":"Fanning Xia,&nbsp;Ana Santacruz ,&nbsp;Di Wu,&nbsp;Sylvain Bertho,&nbsp;Elizabeth Fritz,&nbsp;Pedro Morales-Sosa,&nbsp;Sean McKinney,&nbsp;Stephanie H. Nowotarski,&nbsp;Nicolas Rohner","doi":"10.1016/j.ydbio.2025.04.006","DOIUrl":"10.1016/j.ydbio.2025.04.006","url":null,"abstract":"<div><div>Reproduction is a fundamental biological process for the survival and continuity of species. Examining changes in reproductive strategies offers valuable insights into how animals have adapted their life histories to different environments. Since reproduction is one of the most energy-intensive processes in female animals, nutrient scarcity is expected to interfere with the ability to invest in gametes. Lately, a new model to study adaptation to nutrient limitation has emerged; the Mexican tetra <em>Astyanax mexicanus</em>. This fish species exists as two different morphs, a surface river morph and a cave-dwelling morph. The cave-dwelling morph has adapted to the dark, lower biodiversity, and nutrient-limited cave environment and consequently evolved an impressive starvation resistance. However, how reproductive strategies have adapted to nutrient limitations in this species remains poorly understood. Here, we compared breeding activities and maternal contributions between laboratory-raised surface fish and cavefish. We found that cavefish produce different clutch sizes of eggs with larger yolk compared to surface fish, indicating a greater maternal nutrient deposition in cavefish embryos. To systematically characterize yolk compositions, we used untargeted proteomics and lipidomics approaches to analyze protein and lipid profiles in 2-cell stage embryos and found an increased proportion of sphingolipids in cavefish compared to surface fish. Additionally, we generated transcriptomic profiles of surface fish and cavefish ovaries using a combination of single cell and bulk RNA sequencing to examine differences in maternal contribution. We found that genes essential for hormone regulation were upregulated in cavefish follicular somatic cells compared to surface fish. To evaluate whether these differences contribute to their reproductive abilities under natural-occurring stress, we induced breeding in starved female fish. Remarkably, cavefish maintained their ability to breed under starvation, whereas surface fish largely lost this ability. We identified <em>insulin-like growth factor 1a receptor</em> (<em>igf1ra</em>) as a potential candidate gene mediating the downregulation of ovarian development genes, potentially contributing to the starvation-resistant fertility of cavefish. Taken together, we investigated the female reproductive strategies in <em>Astyanax mexicanus</em>, which will provide fundamental insights into the adaptations of animals to environments with extreme nutrient deficit.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"523 ","pages":"Pages 82-98"},"PeriodicalIF":2.5,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PCP-dependent polarized mechanics in the cortex of individual cells during convergent extension","authors":"Shinuo Weng ,&nbsp;Caitlin C. Devitt ,&nbsp;Bill M. Nyaoga ,&nbsp;José Alvarado ,&nbsp;John B. Wallingford","doi":"10.1016/j.ydbio.2025.04.007","DOIUrl":"10.1016/j.ydbio.2025.04.007","url":null,"abstract":"<div><div>Convergent extension (CE) is a key process for tissue elongation during vertebrate development and is driven by polarized cell behaviors. Here, we used a novel image-based technique to investigate the mechanical properties of individual cells undergoing CE. Our results suggest a PCP- and Septin-dependent mechanical gradient, where cortical tension is higher at the anterior face of the cells compared with their posterior face. Disruption of PCP protein Vangl2 or its downstream effector Septin7 eliminates this mechanical polarity. These findings demonstrate a link between actin organization, PCP signaling, and mechanical polarization, providing new avenues into the mechanochemical regulation of cellular behaviors during CE.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"523 ","pages":"Pages 59-67"},"PeriodicalIF":2.5,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143839508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outside Back Cover - Graphical abstract TOC/TOC in double column/Cover image legend if applicable, Bar code, Abstracting and Indexing information","authors":"","doi":"10.1016/S0012-1606(25)00097-1","DOIUrl":"10.1016/S0012-1606(25)00097-1","url":null,"abstract":"","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"522 ","pages":"Page OBC"},"PeriodicalIF":2.5,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143815009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterisation of the avascular mesenchyme during digit outgrowth","authors":"Cameron Batho-Samblas ,&nbsp;Jonathan Smith ,&nbsp;Lois Keavey ,&nbsp;Noah Clancy ,&nbsp;Lynn McTeir ,&nbsp;Megan G. Davey","doi":"10.1016/j.ydbio.2025.04.005","DOIUrl":"10.1016/j.ydbio.2025.04.005","url":null,"abstract":"<div><div>The avascular mesenchyme at the tip of the developing digit contributes to digit outgrowth and patterning, however, it has been poorly characterised. Using newly developed fate mapping approaches, tissue manipulation and single-cell mRNA sequencing data, we explore the transcriptional nature and developmental potential of this tissue. We find that the avascular mesenchyme is essential to normal segmental patterning of the digit and has a distinct transcriptional identity. In addition, we uncover an unexpected relationship between the unspecified tissue of the avascular mesenchyme and the committed phalanx forming region, which controls patterning, but not outgrowth of the digit. This multifaceted approach provides insights into the mechanics and genetic pathways that regulate digit outgrowth and patterning.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"523 ","pages":"Pages 99-110"},"PeriodicalIF":2.5,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143863603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The neural plate border: multipotent progenitors or cells of mixed identity?","authors":"Andrea Streit","doi":"10.1016/j.ydbio.2025.04.002","DOIUrl":"10.1016/j.ydbio.2025.04.002","url":null,"abstract":"<div><div>The neural plate border is transient territory surrounding the anterior neural plate containing precursors for all ectodermal derivatives: the neural plate, neural crest cells, sensory placodes and the epidermis. A long-standing question is whether its resident cells are already biased to their future identity, whether they represent multipotent progenitor cells and if so, how these lineages segregate. Here, I review the studies that originally defined the neural plate border including lineage tracing, gene expression and functional data. I then discuss how recent single cell analysis has shaped the current view that neural plate border cells are multipotent progenitors as well as future directions to unravel the gene regulatory networks how neural plate border cells diversify.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"523 ","pages":"Pages 51-58"},"PeriodicalIF":2.5,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143833180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of the Magnet-Cre optogenetic system in the chicken model","authors":"Michael Pfann ,&nbsp;Enbal Ben-Tal Cohen ,&nbsp;Dalit Sela-Donenfeld ,&nbsp;Yuval Cinnamon","doi":"10.1016/j.ydbio.2025.04.003","DOIUrl":"10.1016/j.ydbio.2025.04.003","url":null,"abstract":"<div><div>Chickens serve as an excellent model organism for developmental biology, offering unique opportunities for precise spatiotemporal access to embryos within eggs. Optogenes are light-activated proteins that regulate gene expression, offering a non-invasive method to activate genes at specific locations and developmental stages, advancing developmental biology research. This study employed the Magnet-Cre optogenetic system to control gene expression in developing chicken embryos. Magnet-Cre consists of two light-sensitive protein domains that dimerize upon light activation, each attached to an inactive half of the Cre recombinase enzyme, which becomes active upon dimerization.</div><div>We developed an all-in-one plasmid containing a green fluorescent protein marker, the Magnet-Cre system, and a light-activated red fluorescent protein gene. This plasmid was electroporated into the neural tube of Hamburger and Hamilton (H&amp;H) stage 14 chicken embryos. Embryo samples were cleared using the CUBIC protocol and imaged with a light sheet microscope to analyze optogenetic activity via red-fluorescent cells. We established a pipeline for Magnet-Cre activation in chicken embryos, demonstrating that a single 3-min exposure to blue light following incubation at 28 °C was sufficient to trigger gene activity within the neural tube, with increased activity upon additional light exposure. Finally, we showed a spatiotemporal control of gene activity using a localized laser light induction.</div><div>This research lays the groundwork for further advancements in avian developmental biology and poultry research, enabling spatiotemporal control of genes in both embryos and transgenic chickens.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"523 ","pages":"Pages 68-81"},"PeriodicalIF":2.5,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143787906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ASCL1 protein domains with distinct functions in neuronal differentiation and subtype specification","authors":"Yuji Nakada ,&nbsp;Madison J. Martinez ,&nbsp;Jane E. Johnson","doi":"10.1016/j.ydbio.2025.04.001","DOIUrl":"10.1016/j.ydbio.2025.04.001","url":null,"abstract":"<div><div>ASCL1 is a neural basic helix-loop-helix (bHLH) transcription factor that plays essential roles during neural development, including neural differentiation and neuronal subtype specification. bHLH factors are defined by their motifs, including a basic region interacting with DNA and an HLH domain involved in protein-protein interactions. We previously defined specific regions within the bHLH domain of ASCL1 as important for its specific functions directing neuronal differentiation in the chick neural tube. Here, we build upon these findings to show how specific mutations within the basic region block DNA binding but not heterodimer formation with E-protein partners TCF3 (E12/E47) and TCF12 (HEB) yet have differential abilities to show dominant negative phenotypes. Additionally, truncating domains outside the bHLH define a nuclear localization signal, a requirement for the C-terminal acidic residues, and the non-essentiality of the N-terminal glutamine/alanine repeats. This structure/function analysis identifies functional domains for ASCL1 activity.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"523 ","pages":"Pages 32-42"},"PeriodicalIF":2.5,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143787907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of the dysregulated let-7c-Sox2 network in the facial prominences of mouse embryos with early retinoid acid exposure","authors":"Chao Song,&nbsp;Junjie Lu,&nbsp;Ya Wang,&nbsp;Yi Zou","doi":"10.1016/j.ydbio.2025.03.018","DOIUrl":"10.1016/j.ydbio.2025.03.018","url":null,"abstract":"<div><div>RA signaling is crucial for the anteroposterior pattern formation during neural crest induction and acts as a key environmental cue for cranial neural crest cell migration as well as the subsequent mesenchymal proliferation and differentiation. Congenital malformations including cleft lip and palate have been shown associated with altered embryonic RA signaling both in human and in animal models. In this study, a dysregulated <em>let-7c-Sox2</em> network was identified in the altered transcriptomic profiles of the facial prominences of E12.5 mouse embryos induced by early RA exposure. Ubiquitously increased expression of <em>let-7c</em> was observed in the epithelium and the mesenchyme of facial prominences of the RA treated mouse and chick embryos. Direct binding and regulation between <em>let-7c</em> and <em>Sox2</em> was verified using luciferase assay and significant negative correlation between <em>let-7c</em> and <em>Sox2</em> expression was observed <em>in vitro</em>. Reduced <em>Sox2</em> expression was predominantly identified in the epithelium of maxillary and palate shelves from E10.5 to E12.5 in RA-induced mouse embryos, resulted in oral adhesion and hypoplasia of palatal shelves that could partly be explained by the reduced mesenchymal proliferation due to upregulation of <em>let-7c</em>, as shown by the results of cell proliferation assay <em>in vitro</em>.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"523 ","pages":"Pages 9-19"},"PeriodicalIF":2.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143779387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cellular dedifferentiation. Revisiting Betty Hay's legacy","authors":"Alejandra Beltrán-Rivera,&nbsp;José E. García-Arrarás","doi":"10.1016/j.ydbio.2025.03.017","DOIUrl":"10.1016/j.ydbio.2025.03.017","url":null,"abstract":"<div><div>The concept of mature specialized cells and the stability of the differentiated state was fundamentally challenged by Elizabeth Hay's groundbreaking observations on amphibian limb regeneration, published in 1959. Building on previous work by C.S. Thornton, she discovered that muscle cells could dedifferentiate and transform into progenitor cells within the regeneration blastema reshaping our understanding of cell differentiation. This pivotal finding reshaped our understanding of cell differentiation, opening new avenues of research. Though controversial, her findings significantly advanced the fields of cell plasticity and regenerative biology.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"523 ","pages":"Pages 1-8"},"PeriodicalIF":2.5,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143751708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cereblon E3 ligase complex genes are expressed in tissues sensitive to thalidomide in chicken and zebrafish embryos but are unchanged following thalidomide exposure","authors":"Lucas Rosa Fraga ,&nbsp;Jessica Reeves,&nbsp;Chris Mahony ,&nbsp;Lynda Erskine,&nbsp;Neil Vargesson","doi":"10.1016/j.ydbio.2025.03.014","DOIUrl":"10.1016/j.ydbio.2025.03.014","url":null,"abstract":"<div><div>Thalidomide is an infamous drug used initially as a sedative until it was tragically discovered it has highly teratogenic properties. Despite this it is now being used to successfully treat a range of clinical conditions including erythema nodosum leprosum (ENL) and multiple myeloma (MM). Cereblon (CRBN), a ubiquitin ligase, is a binding target of thalidomide for both its therapeutic and teratogenic activities and forms part of an CRL4-E3 ubiquitin ligase complex with the proteins Damaged DNA Binding protein 1 (DDB1) and Cullin-4A (CUL4A). This complex mediates degradation of the zinc-finger transcription factors Ikaros (IKZF1) and Aiolos (IKZF3), to mediate thalidomide's anti-myeloma response. To better understand the importance of CRBN and its binding partners for thalidomide teratogenesis here we analysed the expression patterns of <em>CRBN</em> and some of its known E3 complex binding partners in wildtype and thalidomide-treated chicken and zebrafish embryos. <em>CRBN</em> and <em>DDB1</em> are expressed in many tissues throughout development including those that are thalidomide-sensitive while <em>CUL4A</em> and targets of the CRL4-CRBN E3 Ligase Complex <em>IKZF1</em> and <em>IKZF3</em> are expressed at different timepoints and in fewer tissues in the body than <em>CRBN</em>. Furthermore, <em>IKZF3</em> is expressed in tissues of the eye that <em>CRBN</em> is not. However, although we observed rapid changes to the chicken yolk-sac membrane vasculature following thalidomide exposure, we did not detect CRL4-CRBN E3 Ligase Complex expression in the yolk-sac membrane vessels. Furthermore, we did not detect any changes in <em>CRBN</em>, <em>DDB1</em>, <em>CUL4</em>, <em>IKZF1</em> and <em>IKZF3</em> expression following thalidomide exposure in chicken and zebrafish embryos. These findings demonstrate that the anti-angiogenic activities of thalidomide may occur independent of <em>CRBN</em> and that thalidomide does not regulate CRL4-CRBN E3 Ligase Complex gene expression at the mRNA level.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"522 ","pages":"Pages 156-170"},"PeriodicalIF":2.5,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143746595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}