Developmental biology最新文献_第2页

The primary cilium gene CPLANE1 is required for peripheral nervous system development.

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-12-16 DOI: 10.1016/j.ydbio.2024.12.008

Elkhan Yusifov, Martina Schaettin, Alexandre Dumoulin, Ruxandra Bachmann-Gagescu, Esther T Stoeckli

{"title":"The primary cilium gene CPLANE1 is required for peripheral nervous system development.","authors":"Elkhan Yusifov, Martina Schaettin, Alexandre Dumoulin, Ruxandra Bachmann-Gagescu, Esther T Stoeckli","doi":"10.1016/j.ydbio.2024.12.008","DOIUrl":"10.1016/j.ydbio.2024.12.008","url":null,"abstract":"<p><p>Ciliopathies are a group of neurodevelopmental disorders characterized by the dysfunction of the primary cilium. This small protrusion from most cells of our body serves as a signaling hub for cell-to-cell communication during development. Cell proliferation, differentiation, migration, and neural circuit formation have been demonstrated to depend on functional primary cilia. In the context of ciliopathies, the focus has been on the development of the central nervous system, while the peripheral nervous system has not been studied in depth. In line with phenotypes seen in patients, the absence of a functional primary cilium was shown to affect the migration of cranial and vagal neural crest cells, which contribute to the development of craniofacial features and the heart, respectively. We show here that the ciliopathy gene Cplane1 is required for the development of the peripheral nervous system. Loss of Cplane1 function in chicken embryos induces defects in dorsal root ganglia, which vary in size and fail to localize symmetrically along the spinal cord. These defects may help to understand the alteration in somatosensory perceptions described in some ciliopathy patients.</p>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":" ","pages":"106-121"},"PeriodicalIF":2.5,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142853128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The search to understand the development of the chicken immune system: Differences in expression of MHC class I loci and waves of thymocytes as evolutionary relics?

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-12-16 DOI: 10.1016/j.ydbio.2024.12.006

Samer Halabi, Nicolas Rocos, Jim Kaufman

{"title":"The search to understand the development of the chicken immune system: Differences in expression of MHC class I loci and waves of thymocytes as evolutionary relics?","authors":"Samer Halabi, Nicolas Rocos, Jim Kaufman","doi":"10.1016/j.ydbio.2024.12.006","DOIUrl":"10.1016/j.ydbio.2024.12.006","url":null,"abstract":"<p><p>Chickens are renowned as a model for embryogenesis but have also been responsible for crucial advances in virology, cancer research and immunology. However, chickens are best known as a major source of animal protein for human nutrition, with roughly 80 billion chickens alive each year supplying meat and eggs, the vast majority part of a global poultry industry. As a result, avian immunology been studied intensively for over 60 years, and it has become clear that a major genetic locus in chickens determining resistance to infectious disease and response to vaccines is the major histocompatibility complex (MHC). Compared to typical mammals, the chicken MHC is compact and simple, with only two classical class I genes. A dominantly-expressed class I gene, BF2, is the major ligand for cytotoxic T lymphocytes (CTLs), while the other locus, BF1, is much less well-expressed, lacking in some MHC haplotypes, and is a ligand for natural killer (NK) cells. Cell surface class I expression in neonatal chicks is far less than in adults, and one possibility is that BF2 is not well-expressed early in ontogeny. A precedent is found for amphibians: the single classical class I molecule is not expressed in tadpoles of Xenopus frogs, although non-polymorphic (and thus non-classical) class I molecules from the XNC locus are expressed, which are recognised for immune defence by non-canonical NKT lymphocytes. Indeed, three waves of different T cells are produced by the Xenopus thymus: in tadpoles, during metamorphosis and finally as adults. Three waves of thymic emigrants are also found for chickens, and reasoning by analogy, it may be that the waves of thymocytes and the expression of class I molecules during ontogeny of chickens are evolutionary relics. As well as scientific interest in the ontogeny of MHC class I expression and appearance of peripheral T cells, there are potential practical implications, given the importance of vaccination in ovo and in day-old chicks for the poultry industry.</p>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":" ","pages":"38-45"},"PeriodicalIF":2.5,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142853271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomic analysis and epigenetic regulators in human oocytes at different stages of oocyte meiotic maturation.

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-12-15 DOI: 10.1016/j.ydbio.2024.12.004

Carla Caniçais, Daniel Sobral, Sara Vasconcelos, Mariana Cunha, Alice Pinto, Joana Mesquita Guimarães, Fátima Santos, Alberto Barros, Sofia Dória, C Joana Marques

{"title":"Transcriptomic analysis and epigenetic regulators in human oocytes at different stages of oocyte meiotic maturation.","authors":"Carla Caniçais, Daniel Sobral, Sara Vasconcelos, Mariana Cunha, Alice Pinto, Joana Mesquita Guimarães, Fátima Santos, Alberto Barros, Sofia Dória, C Joana Marques","doi":"10.1016/j.ydbio.2024.12.004","DOIUrl":"10.1016/j.ydbio.2024.12.004","url":null,"abstract":"<p><p>Human oocytes are highly specialized cells with the capacity to store and regulate mRNAs during oocyte maturation, in preparation for post-fertilization steps. Here we performed single-oocyte transcriptomic analysis of human oocytes in three meitoic maturation stages - Germinal Vesicle (GV; n = 6), Metaphase I (MI; n = 6) and Metaphase II (MII; n = 7). Single-oocyte transcriptomic analysis revealed that the total number of expressed genes progressively decreased from GV to MII stages, with 9660 genes being transcribed in GV, 8734 in MI and 5889 in MII. The same tendency was observed for the number of uniquely expressed genes, with 1328 uniquely expressed genes in GV, 401 in MI and 72 in MII. GO analysis of the uniquely expressed genes showed distinct terms in GV oocytes such as transferase activity, organonitrogen compound metabolic process and ncRNA processing. Analysis of Differentially Expressed Genes (DEGs) between the three maturation stages revealed 1165 DEGs between GV and MII oocytes, with 635 being upregulated and 528 downregulated, 42 DEGs between GV and MI, with 38 being upregulated and 4 downregulated, and no significant changes in gene expression between MI and MII oocytes. Comprehensive analysis of epigenetic regulators showed high expression of several histone-modifying enzymes, namely deacetylases, acetylases, lysine demethylases and methyltransferases, and DNA methylation regulators, namely the maintenance methyltransferase DNMT1 and its co-regulators DPPA3 and UHRF1. Some of these epigenetic regulators were differentially expressed between maturation stages, namely SIRT3, SIRT6, KDM3AP1, KMT2E, DNMT1, DPPA3 and the MEST and RASGRF1 imprinted genes. Our study contributes with important information on the transcriptional landscape of human oocytes in different stages of meiotic maturation, providing important insights into candidate biomarkers of human oocyte quality.</p>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":" ","pages":"55-64"},"PeriodicalIF":2.5,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142834407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Automated non-invasive laser speckle imaging of the chick heart rate and extraembryonic blood vessels and their response to Nifedipine and Amlodipine drugs.

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-12-13 DOI: 10.1016/j.ydbio.2024.12.005

Carol Readhead, Simon Mahler, Zhenyu Dong, Yuki Sato, Changhuei Yang, Marianne E Bronner

{"title":"Automated non-invasive laser speckle imaging of the chick heart rate and extraembryonic blood vessels and their response to Nifedipine and Amlodipine drugs.","authors":"Carol Readhead, Simon Mahler, Zhenyu Dong, Yuki Sato, Changhuei Yang, Marianne E Bronner","doi":"10.1016/j.ydbio.2024.12.005","DOIUrl":"10.1016/j.ydbio.2024.12.005","url":null,"abstract":"<p><p>Using our recently developed laser speckle contrast imaging (LSCI) to visualize blood vessels and monitor blood flow noninvasively, we test the utility of the developing chick heart as a functional model for drug screening. To this end, we examined the effects of antihypertensive agents Nifedipine and Amlodipine, belonging to the L-type calcium channel antagonist family, on blood flow visualized noninvasively through the intact shell. Guided by the live view mode, the drugs were injected through the shell and ventral to HH16-19 chick embryos. Our results show a significant reduction in the chick's heart rate, blood flow, and vascular size within 5-20 min after Nifedipine or Amlodipine injection. For moderate Nifedipine concentrations, these parameters returned to initial values within 2-3 h. Nifedipine showed a rapid reduction in heart rate and blood flow dynamics at a concentration ten times lower than Amlodipine. These findings show that our LSCI system can monitor and distinguish the chick heart's response to injected drugs from the same family. This serves as proof-of-concept, paving the way for a rapid, cost-effective, and quantitative test system for screening drugs that affect the cardiovascular system of live chick embryos. Live noninvasive imaging may also provide insights into the development and functioning of the vertebrate heart.</p>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":" ","pages":"46-54"},"PeriodicalIF":2.5,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142827911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Congenital heart defects differ following left versus right avian cardiac neural crest ablation. 左侧和右侧禽类心脏神经嵴消融术后的先天性心脏缺陷不同。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-12-10 DOI: 10.1016/j.ydbio.2024.12.003

Tatiana Solovieva, Marianne E Bronner

引用次数: 0

Development and functions of the area opaca of the chick embryo.

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-12-09 DOI: 10.1016/j.ydbio.2024.12.002

Hyung Chul Lee, Yara Fadaili, Claudio D Stern

引用次数: 0

Decoding a Cell's Fate: How Notch and receptor tyrosine kinase signals specify the Drosophila R7 photoreceptor.

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-12-07 DOI: 10.1016/j.ydbio.2024.12.001

Ronald A Arias, Andrew Tomlinson

{"title":"Decoding a Cell's Fate: How Notch and receptor tyrosine kinase signals specify the Drosophila R7 photoreceptor.","authors":"Ronald A Arias, Andrew Tomlinson","doi":"10.1016/j.ydbio.2024.12.001","DOIUrl":"10.1016/j.ydbio.2024.12.001","url":null,"abstract":"<p><p>The process by which the Drosophila R7 photoreceptor is specified has become a classic model for understanding how cell-cell signals direct cell fates. In the R7 precursor cell, both the Notch and receptor tyrosine kinase (RTK) signaling pathways are active, and the information they encode directs the specification of the R7 photoreceptor identity. In this process, Notch performs three distinct functions: it both opposes and promotes the actions of the RTK pathway to specify the photoreceptor fate, and it determines the type of photoreceptor that is specified. The RTK pathway drives transcription of phyl - a gene expression necessary for photoreceptor specification. We show that Notch activity induces transcription of the yan gene which encodes a transcriptional repressor of phyl. This defines an antagonism between the two pathways, with RTK promoting and Notch opposing phyl transcription. We previously showed that Notch activity supplies Sevenless to the R7 precursor to allow the RTK pathway hyperactivation required to overcome the Notch repression, and we now identify the regulation of Yan activity as a site of integration of RTK and Notch signaling pathways. Once the cell is specified as a photoreceptor, the third Notch function then prevents seven-up (svp) transcription. The Svp transcription factor directs the R1/6 photoreceptor fate, and the prevention of its expression ensures the default R7 specification.</p>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":" ","pages":"21-29"},"PeriodicalIF":2.5,"publicationDate":"2024-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142799657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR-Cas13d as a molecular tool to achieve targeted gene expression knockdown in chick embryos.

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-11-30 DOI: 10.1016/j.ydbio.2024.11.013

Minyoung Kim, Erica J Hutchins

引用次数: 0

A mathematical model of development shows that cell division, short-range signaling and self-activating gene networks increase developmental noise while long-range signaling and epithelial stiffness reduce it.

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-11-30 DOI: 10.1016/j.ydbio.2024.11.014

Hugo Cano-Fernández, Tazzio Tissot, Miguel Brun-Usan, Isaac Salazar-Ciudad

{"title":"A mathematical model of development shows that cell division, short-range signaling and self-activating gene networks increase developmental noise while long-range signaling and epithelial stiffness reduce it.","authors":"Hugo Cano-Fernández, Tazzio Tissot, Miguel Brun-Usan, Isaac Salazar-Ciudad","doi":"10.1016/j.ydbio.2024.11.014","DOIUrl":"10.1016/j.ydbio.2024.11.014","url":null,"abstract":"<p><p>The position of cells during development is constantly subject to noise, i.e. cell-level noise. We do not yet fully understand how cell-level noise coming from processes such as cell division or movement leads to morphological noise, i.e. morphological differences between genetically identical individuals developing in the same environment. To address this question we constructed a large ensemble of random genetic networks regulating cell behaviors (contraction, adhesion, etc.) and cell signaling. We simulated them with a general computational model of development, EmbryoMaker. We identified and studied the dynamics, under cell-level noise, of those networks that lead to the development of animal-like morphologies from simple blastula-like initial conditions. We found that growth by cell division is a major contributor to morphological noise. Self-activating gene network loops also amplified cell-level noise into morphological noise while long-range signaling and epithelial stiffness tended to reduce morphological noise.</p>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":" ","pages":"85-97"},"PeriodicalIF":2.5,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Semaphorin 3A repulsion directs the caudal projection of pioneer longitudinal axons in the developing chicken brain

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-11-28 DOI: 10.1016/j.ydbio.2024.11.012

Kerry-lyn Riley , Susanne Dietrich , Frank R. Schubert

{"title":"Semaphorin 3A repulsion directs the caudal projection of pioneer longitudinal axons in the developing chicken brain","authors":"Kerry-lyn Riley , Susanne Dietrich , Frank R. Schubert","doi":"10.1016/j.ydbio.2024.11.012","DOIUrl":"10.1016/j.ydbio.2024.11.012","url":null,"abstract":"<div><div>The medial longitudinal fasciculus (MLF) is the first axon tract to develop in the ventral vertebrate brain. It originates in the diencephalon and projects caudally into the spinal cord, pioneering the path for later developing axons. Previous anatomical and expression analyses in the chicken suggested Semaphorin 3 A (Sema3A) as the candidate to repel the amniote MLF from the forebrain. However, studies in the zebrafish implicated a distantly related semaphorin with a role in axon fasciculation, not guidance. Thus, the mechanism accounting for the caudal projection of the MLF remains unclear.</div><div>Here we show that misexpression of Sema3A or grafting of Sema3A-expressing cells into the path of the MLF diverts the axons or blocks their outgrowth in chicken embryos. In vitro, Sema3A exposure resulted in the collapse of MLF growth cones. A dominant-negative approach or siRNA to interfere with the function of the Sema3A receptor Neuropilin1 allowed MLF axons to project rostrally. Together, this suggests that Sema3a repulsion directs the caudal extension of the MLF to pioneer the ventral longitudinal tract.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"518 ","pages":"Pages 77-84"},"PeriodicalIF":2.5,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142757418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0