Developmental biology最新文献

{"title":"The yolk sac vasculature in early avian embryo provides a novel model for the analysis of cancer extravasation","authors":"Mizuki Morita ,&nbsp;Ryo Fujii ,&nbsp;Asuka Ryuno ,&nbsp;Manami Morimoto ,&nbsp;Akihito Inoko ,&nbsp;Takahiro Inoue ,&nbsp;Junichi Ikenouchi ,&nbsp;Yuji Atsuta ,&nbsp;Yoshiki Hayashi ,&nbsp;Takayuki Teramoto ,&nbsp;Daisuke Saito","doi":"10.1016/j.ydbio.2025.05.010","DOIUrl":"10.1016/j.ydbio.2025.05.010","url":null,"abstract":"<div><div>Hematogenous metastasis, a hallmark of cancer cells, involves a complex series of migration steps, including intravasation, circulation, arrest in blood vessels, and trans-endothelial migration (TEM)-the lattar two collectively referred to as extravasation. Among these steps, extravasation poses significant challenges for imaging in amniotes such as humans and mice due to its unpredictable timing and location, which limits our understanding of the underlying cellular and molecular mechanisms. Thus, the development of a novel cancer carrier model with high-resolution imaging capabilities in amniotes is essential. In this study, we investigated the yolk sac vasculature (YSV) of early avian embryos (chickens and quail) as an innovative model for studying extravasation, capitalizing on its superior imaging capabilities. We assessed the YSV structure and applied fluorescent labeling to improve visibility. Following this, cancer cells were introduced into the YSV, and their behavior was monitored, revealing distinct morphologies and dynamics associated with extravasation. Furthermore, the YSV model exhibited a high degree of quantitative precision for extravasation studies and demonstrated potential for drug screening applications. Our findings indicate that the YSV model holds promise as a novel platform for elucidating the cellular and molecular mechanisms involved in cancer metastasis through advanced imaging techniques.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"524 ","pages":"Pages 162-175"},"PeriodicalIF":2.5,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Centriolar defects underlie a primary ciliary dyskinesia phenotype in an adenylate kinase 7 deficient ciliated epithelium","authors":"Jennifer Sheridan ,&nbsp;Aline Grata ,&nbsp;Julia Dorr ,&nbsp;Eve E. Suva ,&nbsp;Enzo Bresteau ,&nbsp;Linus R. Mitchell ,&nbsp;Osama Hassan ,&nbsp;Brian Mitchell","doi":"10.1016/j.ydbio.2025.05.011","DOIUrl":"10.1016/j.ydbio.2025.05.011","url":null,"abstract":"<div><div>The skin of <em>Xenopus</em> embryos contains numerous multiciliated cells (MCCs), which collectively generate a directed fluid flow across the epithelial surface essential for distributing the overlaying mucous. MCCs develop into highly specialized cells to generate this flow, containing approximately 150 evenly spaced centrioles that give rise to motile cilia. MCC-driven fluid flow can be impaired when ciliary dysfunction occurs, resulting in primary ciliary dyskinesia (PCD) in humans. Mutations in a large number of genes (∼50) have been found to be causative to PCD. Recently, studies have linked low levels of Adenylate Kinase 7 (AK7) gene expression to patients with PCD; however, the mechanism for this link remains unclear. Additionally, AK7 mutations have been linked to multiple PCD patients. Adenylate kinases modulate ATP production and consumption, with AK7 explicitly associated with motile cilia. Here we reproduce an AK7 PCD-like phenotype in <em>Xenopus</em> and describe the cellular consequences that occur with manipulation of AK7 levels. We show that AK7 localizes throughout the cilia in a DPY30 domain-dependent manner, suggesting a ciliary function. Additionally, we find that AK7 overexpression increases centriole number, suggesting a role in regulating centriole biogenesis. We find that in AK7-depleted embryos, cilia number, length, and beat frequency are all reduced, which in turn significantly decreases the tissue-wide mucociliary flow. Additionally, we find a decrease in centriole number and an increase in sub-apical centrioles, implying that AK7 influences both centriole biogenesis and docking, which we propose underlie its defect in ciliogenesis. We find that both the AK domain and the DPY30 domain are required for proper centriole regulation. We propose that AK7 plays a role in PCD by impacting centriole biogenesis and apical docking, ultimately leading to ciliogenesis defects that impair mucociliary clearance.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"524 ","pages":"Pages 152-161"},"PeriodicalIF":2.5,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Centralspindlin is partially required for C. elegans anchor cell specification, vulval induction and morphogenesis","authors":"Tatsuya Kato ,&nbsp;Olga Skorobogata ,&nbsp;Christian E. Rocheleau","doi":"10.1016/j.ydbio.2025.05.015","DOIUrl":"10.1016/j.ydbio.2025.05.015","url":null,"abstract":"<div><div><em>Caenorhabditis elegans</em> vulval development is a relatively simple model of organ development whereby a signal from the overlying gonad induces three epithelial cells to undergo three rounds of cell division to generate 22 cells that make up the vulva. Specification of the vulval cell fates requires coordination between cell division and cell signaling via LIN-12/Notch and LET-23/EGFR pathways in the somatic gonad and the underlying epithelium. Here we characterize the role of the centralspindlin complex in vulval development. Centralspindlin, a heterotetramer of ZEN-4/KIF23 and CYK-4/RacGAP1, is essential for completion of cytokinesis during early embryonic cell divisions. We found that centralspindlin is required for completion of cytokinesis in the developing somatic gonad and hence specification of the LIN-3/EGF-secreting anchor cell (AC) critical for LET-23/EGFR-mediated vulval induction. However, the requirements for centralspindlin for cytokinesis during postembryonic development are incomplete as the AC is frequently specified, though often binucleate. The presence of the binucleate AC correlates with vulval induction demonstrating that LET-23/EGFR signaling is largely functional. Centralspindlin is also partially required for cytokinesis of the vulval cells where it is required for vulval morphogenesis rather than induction. We also found that the GAP domain of CYK-4/RacGAP1 required for contractile ring assembly during embryonic division is not essential for development of the somatic gonad and the vulva. Thus, there appears to be different requirements for centralspindlin during postembryonic development of the somatic gonad and vulva as compared to early embryogenesis.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"524 ","pages":"Pages 199-209"},"PeriodicalIF":2.5,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coilin-containing nuclear biomolecular condensates in zebra finch Taeniopygia guttata growing oocytes","authors":"Svetlana Galkina,&nbsp;Kseniia Matveeva ,&nbsp;Olga Takki,&nbsp;Valeriia Volodkina,&nbsp;Maria Kulak,&nbsp;Julia Shalutina,&nbsp;Elena Gaginskaya","doi":"10.1016/j.ydbio.2025.05.012","DOIUrl":"10.1016/j.ydbio.2025.05.012","url":null,"abstract":"<div><div>In most animals, oocyte growth is accompanied by genome activation, an increase in nuclear volume, and the formation of various biomolecular condensates (BioMCs) through multivalent interactions involving intrinsically disordered protein regions (IDRs) and phase separation. In this study, we characterize specific nuclear biomolecular condensates (NBioMCs) detectable by light microscopy in the oocytes of the zebra finch (<em>Taeniopygia guttata</em>, Passeriformes, Aves), a model species in genomics and neurobiology. We identified a nucleolus in oocytes at the early diplotene stage and observed numerous NBioMCs that tested positive for coilin in oocytes at the lampbrush stage, a period of active transcription. The coilin-positive NBioMCs may be freely distributed within the nucleus or associated with chromosome centromeres. They share characteristics with several known nuclear structures, including nucleoli (due to the presence of fibrillarin and nucleolin), Cajal bodies (marked by coilin and scaRNA2), interchromatin granule clusters (containing SRSF2), and centromeric protein bodies (CPBs) described in other avian species (exhibiting centromeric localization when chromosome-associated and containing STAG2 and SMC5). However, their specific function in zebra finch oocytes remains unclear and requires further investigation.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"524 ","pages":"Pages 144-151"},"PeriodicalIF":2.5,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leveraging chicken embryos for studying human enhancers","authors":"Ruth M. Williams","doi":"10.1016/j.ydbio.2025.05.009","DOIUrl":"10.1016/j.ydbio.2025.05.009","url":null,"abstract":"<div><div>The dynamic activity of complex gene regulatory networks stands at the core of all cellular functions that define cell identity and behaviour. Gene regulatory networks comprise transcriptional enhancers, acted upon by cell-specific transcription factors to control gene expression in a spatial and temporal specific manner. Enhancers are found in the non-coding genome; pathogenic variants can disrupt enhancer activity and lead to disease. Correlating non-coding variants with aberrant enhancer activity remains a significant challenge. Due to their clinical significance, there is a longstanding interest in understanding enhancer function during early embryogenesis. With the onset of the omics era, it is now feasible to identify putative tissue-specific enhancers from epigenome data. However, such predictions <em>in vivo</em> require validation. The early stages of chick embryogenesis closely parallel those of human, offering an accessible <em>in vivo</em> model in which to assess the activity of putative human enhancer sequences. This review explores the unique advantages and recent advancements in employing chicken embryos to elucidate the activity of human transcriptional enhancers and the potential implications of these findings in human disease.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"524 ","pages":"Pages 123-131"},"PeriodicalIF":2.5,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144068932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outside Back Cover - Graphical abstract TOC/TOC in double column/Cover image legend if applicable, Bar code, Abstracting and Indexing information","authors":"","doi":"10.1016/S0012-1606(25)00126-5","DOIUrl":"10.1016/S0012-1606(25)00126-5","url":null,"abstract":"","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"523 ","pages":"Page OBC"},"PeriodicalIF":2.5,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143928632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifaceted roles of sonic hedgehog signaling in mammalian inner ear development","authors":"Tianli Qin,&nbsp;Marianne E. Bronner","doi":"10.1016/j.ydbio.2025.05.007","DOIUrl":"10.1016/j.ydbio.2025.05.007","url":null,"abstract":"<div><div>The inner ear is an intricate structure that houses six sensory organs responsible for both hearing and balance. The development of the inner ear begins with the formation of the otic placode, a transient ectodermal thickening that emerges early during embryonic development. The otic placode undergoes a series of morphological changes, from thickening to invagination and then pinching off from the ectoderm to form the otic vesicle, which further differentiates into the specialized structures of the inner ear. These developmental processes require a coordinated interplay between intrinsic transcription factors and extrinsic signaling molecules, which regulate the patterning, proliferation, and differentiation of the inner ear components. In this review, we focus on the role of Sonic hedgehog (Shh) signaling in the development of the mammalian inner ear. We explore how Shh signaling is involved at multiple stages of inner ear development, including the patterning of the otic vesicle and the differentiation of specific cell types within the cochlea. Additionally, we discuss the mechanisms by which Shh signaling integrates with other signaling pathways and transcription factors to ensure the proper development and function of the inner ear. Understanding the molecular basis of these processes provides valuable insights into inner ear development and its disorders.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"524 ","pages":"Pages 97-104"},"PeriodicalIF":2.5,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bones, brains, and bias—neural crest cell contribution to craniofacial structure","authors":"Jeffrey S. Carmichael ,&nbsp;Diane C. Darland ,&nbsp;Daphne E. Pedersen ,&nbsp;Rebecca B. Simmons","doi":"10.1016/j.ydbio.2025.05.008","DOIUrl":"10.1016/j.ydbio.2025.05.008","url":null,"abstract":"<div><div>Implicit bias is a natural part of the human psyche and facial appearance is one of the first aspects that people notice when encountering new individuals. The cranial neural crest is key to formation of the facial structure as it is a developmentally transient and plastic cell population that contributes to the neural, muscular, cartilage and bone structures of the face and head. This remarkable cell population, within the context of genetic potential, can play a substantial role in determining how individuals’ faces and heads form as well as contributing to anterior brain development. How humans interpret face and head features can lead to biased perceptions, including perceived cognitive ability associated with differences across craniofacial phenotypes. In this Opinion Article, we offer strategies to introduce students to an overview of cranial neural crest development linked to primary research in model organisms and in humans. We then bridge this knowledge with a follow-up activity to foster awareness of cognitive processes and implicit bias in human perception. We provide explicit Learning Goals and guided learning strategies to achieve clear Learning Outcomes. We have developed critical thinking assignments and self-reflection opportunities to engage students and shed light on misconceptions regarding craniofacial features. By introducing the generalized processes whereby neural crest cells contribute to head and face formation, we provide an opportunity to focus on bones, brains, and bias in development in order to encourage students to consider how implicit bias shapes human interaction and scientific work.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"524 ","pages":"Pages 116-122"},"PeriodicalIF":2.5,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Geometric, cell cycle and maternal-to-zygotic transition-associated YAP dynamics during preimplantation embryo development","authors":"Madeleine Chalifoux ,&nbsp;Maria Avdeeva ,&nbsp;Eszter Posfai","doi":"10.1016/j.ydbio.2025.05.006","DOIUrl":"10.1016/j.ydbio.2025.05.006","url":null,"abstract":"<div><div>During the first cell fate decision in mammalian embryos, the inner cell mass cells, which will give rise to the embryo proper and other extraembryonic tissues, segregate from the trophectoderm cells, the precursors of the placenta. Cell fate segregation proceeds in a gradual manner encompassing two rounds of cell division, as well as cell positional and morphological changes. While it is known that the activity of the Hippo signaling pathway and the subcellular localization of its downstream effector YAP dictate lineage specific gene expression, the response of YAP to these dynamic cellular changes remains incompletely understood. Here we address these questions by quantitative live imaging of endogenously tagged YAP while simultaneously monitoring geometric cellular features and cell cycle progression throughout cell fate segregation. We apply a probabilistic model to our dynamic data, providing a quantitative characterization of the mutual effects of YAP and cellular relative exposed area, which has previously been shown to correlate with subcellular YAP localization in fixed samples. Additionally, we study how nuclear YAP levels are influenced by other factors, such as the decreasing pool of maternally provided YAP that is partitioned to daughter cells through cleavage divisions, cell cycle-associated nuclear volume changes, and a delay after divisions in adjusting YAP levels to new cell positions. Interestingly, we find that establishing low nuclear YAP levels required for the inner cell mass fate is largely achieved by passive cell cycle-associated mechanisms. Moreover, contrary to expectations, we find that mechanical perturbations that result in cell and nuclear shape changes do not influence YAP localization in the embryo. Together our work identifies how various inputs are integrated over a dynamic developmental time course to shape the levels of a key molecular determinant of the first cell fate choice.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"524 ","pages":"Pages 105-115"},"PeriodicalIF":2.5,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143987083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estrogen-related receptor is required in adult Drosophila females for germline stem cell maintenance","authors":"Anna B. Zike,&nbsp;Madison G. Abel,&nbsp;Sophie A. Fleck,&nbsp;Emily D. DeWitt,&nbsp;Lesley N. Weaver","doi":"10.1016/j.ydbio.2025.05.005","DOIUrl":"10.1016/j.ydbio.2025.05.005","url":null,"abstract":"<div><div>Adult tissue function is dependent on intrinsic factors that mediate stem cell self-renewal and proliferation in response to changes in physiology and the environment. The estrogen-related receptor (ERR) subfamily of orphan nuclear receptors are major transcriptional regulators of metabolism and animal physiology. In mammals, ERRs (NR3B1, NR3B2, NR3B3) have roles in regulating mitochondrial biosynthesis, lipid metabolism, as well as stem cell maintenance. The sole <em>Drosophila ERR</em> ortholog promotes larval growth by establishing a metabolic state during the latter half of embryogenesis. In addition, <em>ERR</em> is required in adult <em>Drosophila</em> males to coordinate glycolytic metabolism with lipid synthesis and within the testis to regulate spermatogenesis gene expression and fertility. Despite extensive work characterizing the role of <em>ERR</em> in <em>Drosophila</em> metabolism, whether <em>ERR</em> has a conserved requirement in regulating stem cell behavior has been understudied. To determine whether ERR regulates stem cell activity in <em>Drosophila</em>, we used the established adult female germline stem cell (GSC) lineage as a model. We found that whole-body <em>ERR</em> knockout in adult females using conditional heat shock-driven FLP-FRT recombination significantly decreases GSC number and glycolytic enzyme expression in GSCs. In addition, we found that ERR activity is required cell-autonomously in the adult female germline for maintenance of GSCs; whereas ERR regulation of GSCs is independent of its activity in adult female adipocytes. Our results highlight an ancient and conserved role for <em>ERRs</em> in the regulation of stem cell self-renewal.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"524 ","pages":"Pages 132-143"},"PeriodicalIF":2.5,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}