Developmental biology最新文献

{"title":"Organizational principles of integumentary organs: Maximizing variations for effective adaptation","authors":"Cheng Ming Chuong,&nbsp;Ping Wu,&nbsp;Zhou Yu,&nbsp;Ya-Chen Liang,&nbsp;Randall B. Widelitz","doi":"10.1016/j.ydbio.2025.03.011","DOIUrl":"10.1016/j.ydbio.2025.03.011","url":null,"abstract":"<div><div>The integument serves as the interface between an organism and its environment. It primarily comprises ectoderm-derived epithelium and mesenchyme derived from various embryonic sources. These integumentary organs serve as a barrier defining the physiological boundary between the internal and exterior environments and fulfill diverse functions. How does the integument generate such a large diversity? Here, we attempt to decipher the organizational principles. We focus on amniotes and use appendage follicles as the primary examples. The integument begins as a simple planar sheet of coupled epithelial and mesenchymal cells, then becomes more complex through the following patterning processes. 1) <em>De novo Turing periodic patterning process</em>: This process converts the integument into multiple skin appendage units. 2) <em>Adaptive patterning process:</em> Dermal muscle, blood vessels, adipose tissue, and other components are assembled and organized around appendage follicles when present. 3) <em>Cyclic renewal</em>: Skin appendage follicles contain stem cells and their niches, enabling physiological molting and regeneration in the adult animal. 4) <em>Spatial variations</em>: Multiple appendage units allow modulation of shape, size, keratin types, and color patterns of feathers and hairs across the animal's surface. 5) <em>Temporal phenotypic plasticity</em>: Cyclic renewal permits temporal transition of appendage phenotypes, i.e. <em>regulatory patterning</em> or integumentary metamorphosis, throughout an animal's lifetime. The diversities in (4) and (5) can be generated epigenetically within the same animal. Over the evolutionary timescale, different species can modulate the number, size, and distributions of existing ectodermal organs in the context of micro-evolution, allowing effective adaptation to new climates as seen in the variation of hair length among mammals. Novel ectodermal organs can also emerge in the context of macro-evolution, enabling animals to explore new ecological niches, as seen in the emergence of feathers on dinosaurs. These principles demonstrate how multi-scale organ adaption in the amniotes can maximize diverse and flexible integumentary organ phenotypes, producing a vast repertoire for natural selection and thereby providing effective adaptation and evolutionary advantages.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"522 ","pages":"Pages 171-195"},"PeriodicalIF":2.5,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143669412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bud, branch, breathe! Building a mammalian lung over space and time","authors":"Brigid L.M. Hogan","doi":"10.1016/j.ydbio.2025.03.010","DOIUrl":"10.1016/j.ydbio.2025.03.010","url":null,"abstract":"<div><div>Many mammalian organs, such as the mammary and lachrymal glands, kidney and lungs develop by the process known as branching morphogenesis. An essential feature of this process is the reciprocal interaction between the inner branched tubular epithelium and the surrounding mesenchyme to optimize the final amount of epithelial tissue that is generated for specific functions. To achieve this expansion the initial epithelial population undergoes repeated rounds of bud formation, branch outgrowth and tip bifurcations, with each repertoire requiring dynamic changes in cell behavior. The process of branching morphogenesis was first studied experimentally by Grobstein and others who showed that the embryonic epithelium did not develop without so-called inductive signals from the mesenchyme. However, it was not known whether this activity was uniformly distributed throughout the mesoderm or localized to specific regions. The mouse lung was seen as a powerful system in which to investigate such questions since its early branching is highly stereotypic, both <em>in vivo</em> and in culture. This advantage was exploited by two young scientists, Alescio and Cassini, who used grafting techniques with explanted embryonic mouse lungs. They showed that mesenchyme from around distal buds could induce ectopic buds in the trachea and other non-branching regions of the epithelium. At the same time, distal regions denuded of their mesoderm failed to develop further. They speculated that inductive factors that promote bud formation and continued outgrowth in competent endoderm are specifically localized within the distal mesenchyme, establishing a conceptual framework for future experimentation. Since then, advances in many areas of biology and bioengineering have enabled the identification of gene regulatory networks, signaling pathways and biomechanical properties that mediate lung branching morphogenesis. However, a quantitative model of how these parameters are coordinated over space and time to control the pattern and scale of branching and the overall size of the lung, still remains elusive.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"522 ","pages":"Pages 64-75"},"PeriodicalIF":2.5,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A gene regulatory network for specification and morphogenesis of a Mauthner Cell homolog in non-vertebrate chordates","authors":"Kwantae Kim,&nbsp;Katarzyna M. Piekarz,&nbsp;Alberto Stolfi","doi":"10.1016/j.ydbio.2025.03.007","DOIUrl":"10.1016/j.ydbio.2025.03.007","url":null,"abstract":"<div><div>Transcriptional regulation of gene expression is an indispensable process in multicellular development, yet we still do not fully understand how the complex networks of transcription factors operating in neuronal precursors coordinately control the expression of effector genes that shape morphogenesis and terminal differentiation. Here we break down in greater detail a provisional regulatory circuit downstream of the transcription factor Pax3/7 operating in the descending decussating neurons (ddNs) of the tunicate <em>Ciona robusta.</em> The ddNs are a pair of hindbrain neurons proposed to be homologous to the Mauthner cells of anamniotes, and Pax3/7 is sufficient and necessary for their specification. We show that different transcription factors downstream of Pax3/7, namely Pou4, Lhx1/5, and Dmbx, regulate distinct “branches” of this ddN network that appear to be dedicated to different developmental tasks. Some of these network branches are shared with other neurons throughout the larva, reinforcing the idea that modularity is likely a key feature of such networks. We discuss these ideas and their evolutionary implications here, including the observation that homologs of all four transcription factors (Pax3/7, Lhx5, Pou4f3, and Dmbx1) are key for the specification of cranial neural crest in vertebrates.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"522 ","pages":"Pages 51-63"},"PeriodicalIF":2.5,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143643210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Teaching developmental biology to drive social change: Pedagogy that challenges biologically deterministic views on phenotypic variation","authors":"Julia Paxson","doi":"10.1016/j.ydbio.2025.03.004","DOIUrl":"10.1016/j.ydbio.2025.03.004","url":null,"abstract":"<div><div>Discrimination against groups of people based on socially-normed phenotypic variations is commonplace in many societies. The stigmatized phenotypic variations are dependent on specific societal norms but might include features that align with social constructs of race, phenotypic variations that may result in different ability levels, or those that align with social constructs of sex and/or gender identity. Science has contributed to this discrimination through biological essentialism, either by assigning specific undesirable biological characteristics to socially-normed phenotypic groupings, or more recently by assigning a genetic basis for these phenotypic differences. Biological essentialism can promote deterministic views lead to alienation and the persistence of social hierarchies. To overcome this, scientists have a responsibility to create positive changes to decenter practices that contribute to such discrimination. The study of developmental biology straddles the intersection of many biological concepts that have social and political ramifications. This paper outlines a pedagogical approach to create connections between concepts central to developmental biology and broader social issues to which they relate using a biocultural perspective. Specifically, the focus will be on understanding how phenotypes are generated through a combination of biological, environmental and social factors; exploring how deterministic views of biological essentialism contribute to social hierarchies and discrimination (such as racism, sexism, genderism and ableism); and understanding how this discrimination can become embodied through negative chronic and transgenerational biological consequences for stigmatized groups.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"522 ","pages":"Pages 116-124"},"PeriodicalIF":2.5,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143647610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Avian bioresources for developmental biology: Transgenic chicken and quail resources in the United Kingdom, France, and Japan” [Dev. Biol. 521 (2025) 1–13]","authors":"Lindsay Henderson ,&nbsp;Yuya Okuzaki ,&nbsp;Christophe Marcelle ,&nbsp;Mike J. McGrew ,&nbsp;Ken-ichi Nishijima","doi":"10.1016/j.ydbio.2025.03.003","DOIUrl":"10.1016/j.ydbio.2025.03.003","url":null,"abstract":"","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"522 ","pages":"Page 30"},"PeriodicalIF":2.5,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143601873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outside Back Cover - Graphical abstract TOC/TOC in double column/Cover image legend if applicable, Bar code, Abstracting and Indexing information","authors":"","doi":"10.1016/S0012-1606(25)00065-X","DOIUrl":"10.1016/S0012-1606(25)00065-X","url":null,"abstract":"","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"521 ","pages":"Page OBC"},"PeriodicalIF":2.5,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143594104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Teaching the science of life: A multidisciplinary educational approach to reproductive technology debates through the lens of developmental biology","authors":"Jovany Azzi ,&nbsp;Zeinab Wehbi ,&nbsp;Philippe Hussein Kobeissy ,&nbsp;Racha Kerek","doi":"10.1016/j.ydbio.2025.03.005","DOIUrl":"10.1016/j.ydbio.2025.03.005","url":null,"abstract":"<div><div>In vitro fertilization, cryopreservation and pre-implantation genetic testing are transformative reproductive technologies offering hope for individuals facing fertility challenges. Scientifically, understanding the science of developmental biology is essential for comprehending the mechanisms and implications of these technologies. In embryological sciences, biological perspectives identify life as a series of developmental stages ranging from conception to viability, each potentially representing a different ‘beginning’ of life. However, the concept of ensoulment, rooted in religious and cultural beliefs, introduces a speculative dimension ultimately influencing how legal systems worldwide define and protect human life in the context of reproductive decision-making. Legally, high-profile cases such as ‘Sofia Vergara v. Nick Loeb’ or ‘Quintavalle v. Human Fertilization and Embryology Authority’ raise questions about both parental and fetal reproductive rights and consent. This paper highlights the importance of a multidisciplinary approach in developmental biology education for responsible and equitable practices of reproductive technologies. It underscores the importance of incorporating these considerations into the classroom teaching as case study discussions aligned with the DEI approach, to better equip students for the controversies they may encounter in their roles of developmental biologists.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"522 ","pages":"Pages 31-39"},"PeriodicalIF":2.5,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143613588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Geldanamycin inhibits in vivo and in vitro chick skeletal myogenesis","authors":"Kayo Moreira Bagri ,&nbsp;Bruna Vessados Aprigio ,&nbsp;Matthias Guillo,&nbsp;José Brito,&nbsp;Manoel Luis Costa,&nbsp;Claudia Mermelstein","doi":"10.1016/j.ydbio.2025.02.018","DOIUrl":"10.1016/j.ydbio.2025.02.018","url":null,"abstract":"<div><div>During skeletal muscle development catabolic and anabolic events are finely orchestrated by several heat shock proteins (HSP). HSP90 are molecular chaperones which play an essential role in maintaining cellular homeostasis. Although HSP90 proteins have been widely studied in cancer cells, their role during skeletal myogenesis has not been completely explored. Here, we studied the role of HSP90 during <em>in vivo and in vitro</em> development of embryonic chick muscle fibers. First, we analyzed the effects of the inhibition of HSP90 by geldanamycin in skeletal muscle tissues in chick embryos <em>in vivo</em>. Geldanamycin was applied to E2 chicken embryos <em>in vivo</em>, and six days later, we observed a reduction in desmin-positive cells in the pectoral muscle. To deeper analyze the role of HSP90 during myogenesis, we used primary cultures of chick pectoral muscle cells which contain myoblasts, myotubes and muscle fibroblasts. We observed an intense accumulation of HSP90 in the perinuclear region of myoblasts and myotubes, but not in muscle fibroblasts. In myotubes, besides the perinuclear localization, HSP90 was found colocalized with myofibers, whereas in myoblasts and fibroblasts HSP90 was found as a mesh of small filaments and aggregates in the cytoplasm. Fibroblasts showed lower HSP90 expression, as compared to myoblasts and myotubes. Inhibition of HSP90 by geldanamycin reduced the size of myotubes, the number of nuclei within myotubes and myoblast fusion index, indicating an inhibition of myogenesis. Furthermore, geldanamycin induces both an increase in the number of fibroblasts and the formation of giant binucleated fibroblasts, suggesting that HSP90 inhibition induces cytokinesis block in muscle fibroblasts by interfering in actin and myosin activity. The collection of our results shows that the inhibition of HSP90 hinders <em>in vivo</em> and <em>in vitro</em> chick skeletal myogenesis, and differentially affects muscle and non-muscle cells, underlying different functions of HSP90 in different cell types.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"522 ","pages":"Pages 20-29"},"PeriodicalIF":2.5,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A career as a biologist-a lifelong morphogenetic process","authors":"David R. Sherwood","doi":"10.1016/j.ydbio.2025.03.002","DOIUrl":"10.1016/j.ydbio.2025.03.002","url":null,"abstract":"<div><div>How life emerged, how species diversify and interact, how cells work individually and collectively, and how a single cell gives rise to the trillions that constitute us all, are some of life's most profound questions. For the last 20 years, I've been a professor in the Biology Department at Duke University and spent summers teaching at the Marine Biological Laboratory in Woods Hole. I use the small nematode worm <em>C</em>. <em>elegans</em> in my research to understand cell-extracellular matrix interactions, and I've taught and mentored hundreds of undergraduate students, graduate students, postdocs and many junior faculty. Young scientists often become discouraged during their training—the modest pay, the long hours, and the herculean task and many setbacks that occur conducting research, writing grants and papers, teaching, and managing a lab. It can seem to be an impossible challenge and leads many young scientists to curtail their training and leave fulfilling scientific careers. A core element to success is that scientific skills and expertise evolve slowly over an entire career through engagement—learning by doing—and requires patience, acceptance of failures, continual exploration, and a flexible growth mindset. Everyone also takes a unique path and there is no simple rulebook. This is difficult to convey through courses and workshops. By sharing some tools and approaches I've learned through my own setbacks, observations, and reading, I hope to help junior scientists view challenges in this career more as lifelong opportunities for building new skillsets and growth, much like the emergence of form and function that occurs during developmental morphogenesis. Embracing constant change and evolution can lead to a deeply meaningful and fulfilling career.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"522 ","pages":"Pages 1-3"},"PeriodicalIF":2.5,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143579817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The anlagen of evo-devo in Fritz Müller's Für Darwin (1864)","authors":"Scott F. Gilbert ,&nbsp;Beatrice Steinert","doi":"10.1016/j.ydbio.2025.03.001","DOIUrl":"10.1016/j.ydbio.2025.03.001","url":null,"abstract":"<div><div><em>Für Darwin</em>, written in the early 1860s by the German zoologist and Darwinist Fritz Müller, articulates many of the concepts foundational to the contemporary field of evolutionary developmental biology, or evo-devo. Working on the Brazilian coast offered him refuge from both religious conservatism and the “great market\" of Prussian academic science. Here, Müller studied the developmental stages of crustacea and used these meticulous observations to critique the extant literature on classification. In so doing, he both provided evidence for Darwin's theory, and extended it to larval forms. In this essay, we situate <em>Für Darwin</em>, published in English as <em>Facts and Arguments for Darwin</em> in 1869, within the landscape of nineteenth century biology. We propose that <em>Für Darwin</em> is a remarkably prophetic text in the history of developmental biology given its sharp insight into the relationship between development and evolution (ontogeny and phylogeny), its many contributions to crustacean biology, and Müller's deep appreciation of the danger of scientific dogma.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"522 ","pages":"Pages 4-7"},"PeriodicalIF":2.5,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}