Developmental biology最新文献_第3页

Generation of a transgenic chicken line with reporters for limb bud mesenchyme and apical ectodermal ridge cells 具有肢芽间充质和顶端外胚层脊细胞报告基因的转基因鸡系的产生。

IF 2.5 3区生物学

Developmental biology Pub Date : 2025-01-08 DOI: 10.1016/j.ydbio.2025.01.003

Yuji Atsuta , Yi-Chen Chen , Yuna Hattori , Tatsuya Takemoto , Daisuke Saito

引用次数: 0

Foxm1 promotes differentiation of neural progenitors in the zebrafish inner ear Foxm1促进斑马鱼内耳神经祖细胞的分化。

IF 2.5 3区生物学

Developmental biology Pub Date : 2025-01-04 DOI: 10.1016/j.ydbio.2025.01.001

Maria Ali, James W. Kutlowski, Jorden N. Holland, Bruce B. Riley

{"title":"Foxm1 promotes differentiation of neural progenitors in the zebrafish inner ear","authors":"Maria Ali, James W. Kutlowski, Jorden N. Holland, Bruce B. Riley","doi":"10.1016/j.ydbio.2025.01.001","DOIUrl":"10.1016/j.ydbio.2025.01.001","url":null,"abstract":"<div><div>During development of the vertebrate inner ear, sensory epithelia and neurons of the statoacoustic ganglion (SAG) arise from lineage-restricted progenitors that proliferate extensively before differentiating into mature post-mitotic cell types. Development of progenitors is regulated by Fgf, Wnt and Notch signaling, but how these pathways are coordinated to achieve an optimal balance of proliferation and differentiation is not well understood. Here we investigate the role in zebrafish of Foxm1, a transcription factor commonly associated with proliferation in developing tissues and tumors. Targeted knockout of <em>foxm1</em> causes no overt defects in development. Homozygous mutants are viable and exhibit no obvious defects except male sterility. However, the mutant allele acts dominantly to reduce accumulation of SAG neurons, and maternal loss-of-function slightly enhances this deficiency. Neural progenitors are specified normally but, unexpectedly, persist in an early state of rapid proliferation and are delayed in differentiation. Progenitors eventually shift to a slower rate of proliferation similar to wild-type and differentiate to produce a normal number of SAG neurons, although the arrangement of neurons remains variably disordered. Mutant progenitors remain responsive to Fgf and Notch, as blocking these pathways partially alleviates the delay in differentiation. However, the ability of elevated Wnt/beta-catenin to block neural specification is impaired in <em>foxm1</em> mutants. Modulating Wnt at later stages has no effect on progenitors in mutant or wild-type embryos. Our findings document an unusual role for <em>foxm1</em> in promoting differentiation of SAG progenitors from an early, rapidly dividing phase to a more mature slower phase prior to differentiation.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"520 ","pages":"Pages 21-30"},"PeriodicalIF":2.5,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142946001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tunicate-specific protein Epi-1 is essential for conferring hydrophilicity to the larval tunic in the ascidian Ciona 被膜特异性蛋白Epi-1是赋予海鞘幼虫被膜亲水性所必需的。

IF 2.5 3区生物学

Developmental biology Pub Date : 2025-01-04 DOI: 10.1016/j.ydbio.2025.01.002

Kazu Kuroiwa , Kaoru Mita-Yoshida , Mayuko Hamada , Akiko Hozumi , Atsuo S. Nishino , Yasunori Sasakura

{"title":"Tunicate-specific protein Epi-1 is essential for conferring hydrophilicity to the larval tunic in the ascidian Ciona","authors":"Kazu Kuroiwa , Kaoru Mita-Yoshida , Mayuko Hamada , Akiko Hozumi , Atsuo S. Nishino , Yasunori Sasakura","doi":"10.1016/j.ydbio.2025.01.002","DOIUrl":"10.1016/j.ydbio.2025.01.002","url":null,"abstract":"<div><div>Animals must avoid adhesion to objects in the environment to maintain their mobility and independence. The marine invertebrate chordate ascidians are characterized by an acellular matrix tunic enveloping their entire body for protection and swimming. The tunic of ascidian larvae consists of a surface cuticle layer and inner matrix layer. Hydrophilic substances coat the cuticle; this modification is thought to be for preventing adhesion. However, the molecule responsible for regulating this modification has not been clarified. We here found that the tunicate-specific protein Epi-1 is responsible for preventing adhesiveness of the tunic in the ascidian <em>Ciona intestinalis</em> Type A. <em>Ciona</em> mutants with homozygous knockouts of <em>Epi-1</em> exhibited adhesion to plastic plates and to other individuals. The cuticle of the <em>Epi-1</em> mutants was fragile, and it lost the glycosaminoglycans supplied by test cells, the accessory cells that normally attach to the tunic surface. Although it has an apparent signal peptide for membrane trafficking, we showed that the Epi-1 protein is localized to the cytosol of the epidermal cells. Our study suggests that the emergence of the tunicate-specific protein Epi-1 made the tunic less adhesive, providing a selective advantage for the last common tunicate ancestor.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"520 ","pages":"Pages 41-52"},"PeriodicalIF":2.5,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142962135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Moderate levels of folic acid benefit outcomes for cilia based neural tube defects 中等水平的叶酸对基于纤毛的神经管缺陷有好处。

IF 2.5 3区生物学

Developmental biology Pub Date : 2025-01-03 DOI: 10.1016/j.ydbio.2024.12.019

David Engelhardt , Juliette R. Petersen , Cara Martyr , Hannah Kuhn-Gale , Lee A. Niswander

{"title":"Moderate levels of folic acid benefit outcomes for cilia based neural tube defects","authors":"David Engelhardt , Juliette R. Petersen , Cara Martyr , Hannah Kuhn-Gale , Lee A. Niswander","doi":"10.1016/j.ydbio.2024.12.019","DOIUrl":"10.1016/j.ydbio.2024.12.019","url":null,"abstract":"<div><div>Folic acid (FA) supplementation is a potent tool to reduce devastating birth defects known as neural tube defects (NTDs). Though effective, questions remain how FA achieves its protective effect and which gene mutations are sensitive to folic acid levels. We explore the relationship between FA dosage and NTD rates using NTD mouse models. We demonstrate that NTD rates in mouse models harboring mutations in cilia genes depend on FA dosage. Cilia mutant mouse models demonstrate reductions in NTD rates when exposed to moderate levels of FA that are not observed at higher fortified levels of FA. This trend continues with a moderate level of FA being beneficial for primary and motile cilia formation. We present a mechanism through which fortified FA levels reduce basal levels of reactive oxygen species (ROS) which in turn reduces ROS-sensitive GTPase activity required for ciliogenesis. Our data indicates that genes involved in cilia formation and function represent a FA sensitive category of mutations and a possible avenue for further reducing NTD and ciliopathy incidences.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"520 ","pages":"Pages 62-74"},"PeriodicalIF":2.5,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142926904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ancient emergence of neuronal heterogeneity in the enteric nervous system of jawless vertebrates 古代无颌脊椎动物肠神经系统中神经元异质性的出现。

IF 2.5 3区生物学

Developmental biology Pub Date : 2025-01-03 DOI: 10.1016/j.ydbio.2024.12.020

Brittany M. Edens, Jason Lin, Marianne E. Bronner

引用次数: 0

Outside Back Cover - Graphical abstract TOC/TOC in double column/Cover image legend if applicable, Bar code, Abstracting and Indexing information

IF 2.5 3区生物学

Developmental biology Pub Date : 2025-01-01 DOI: 10.1016/S0012-1606(24)00272-0

引用次数: 0

Spatiotemporal control of cell ablation using Ronidazole with Nitroreductase in Drosophila 硝基还原酶Ronidazole对果蝇细胞消融的时空控制。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-12-28 DOI: 10.1016/j.ydbio.2024.12.017

Gary Teeters , Christina E. Cucolo , Sagar N. Kasar , Melanie I. Worley , Sarah E. Siegrist

{"title":"Spatiotemporal control of cell ablation using Ronidazole with Nitroreductase in Drosophila","authors":"Gary Teeters , Christina E. Cucolo , Sagar N. Kasar , Melanie I. Worley , Sarah E. Siegrist","doi":"10.1016/j.ydbio.2024.12.017","DOIUrl":"10.1016/j.ydbio.2024.12.017","url":null,"abstract":"<div><div>The ability to induce cell death in a controlled stereotypic manner has led to the discovery of evolutionary conserved molecules and signaling pathways necessary for tissue growth, repair, and regeneration. Here we report the development of a new method to genetically induce cell death in a controlled stereotypic manner in <em>Drosophila</em>. This method has advantages over other current methods and relies on expression of the <em>E. coli</em> enzyme Nitroreductase (NTR) with exogenous application of the nitroimidazole prodrug, Ronidazole. NTR expression is controlled spatially using the GAL4/UAS system while temporal control of cell death is achieved through timed feeding of Ronidazole supplied in the diet. In cells expressing NTR, Ronidazole is converted to a toxic substance inducing DNA damage and cell death. Caspase cell death is achieved in a range of NTR-expressing cell types with Ronidazole feeding, including epithelial, neurons, and glia. Removing Ronidazole from the diet restores cell death to normal unperturbed levels. Unlike other genetic ablation methods, temporal control is achieved through feeding not temperature, circumventing developmental complications associated with temperature changes. Ronidazole-NTR also requires only two transgenes, a GAL4 driver and <em>UAS-NTR</em>, which is generated as a GFP-NTR fusion allowing for easy setup of large-scale screening of <em>UAS-RNAi</em> lines. Altogether, Ronidazole-NTR provides a new streamlined method for inducing cell death in <em>Drosophila</em> with temperature-independent ON/OFF control.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"520 ","pages":"Pages 31-40"},"PeriodicalIF":2.5,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142906699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Introduction to “Research that Transformed Developmental Biology” 改变发育生物学的研究 "简介。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-12-27 DOI: 10.1016/j.ydbio.2024.12.011

Steven L. Klein Ph.D.

引用次数: 0

Teaching students to effectively evaluate scientific evidence and advocate for research in the context of autism spectrum disorder and the neurodiversity movement 在自闭症谱系障碍和神经多样性运动的背景下，教学生有效地评估科学证据并倡导研究。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-12-27 DOI: 10.1016/j.ydbio.2024.12.015

Bridget T. Jacques-Fricke

{"title":"Teaching students to effectively evaluate scientific evidence and advocate for research in the context of autism spectrum disorder and the neurodiversity movement","authors":"Bridget T. Jacques-Fricke","doi":"10.1016/j.ydbio.2024.12.015","DOIUrl":"10.1016/j.ydbio.2024.12.015","url":null,"abstract":"<div><div>Connecting socially relevant topics with biological content can boost student engagement and comprehension. Autism Spectrum Disorder (ASD) is an increasingly prevalent diagnosis with a number of intersecting topic areas between developmental biology and social justice. Here I describe two exercises that I developed to engage students in learning opportunities that link scientific process learning goals with real-world applications. First, students examine scientific research practices and work on connecting scientific evidence with conclusions by evaluating the retracted 1998 article by Andrew Wakefield that falsely linked the measles, mumps and rubella vaccination with the development of ASD. Second, students participate in a role-playing exercise to learn about the multiple viewpoints and perspectives that are involved in determining funding levels for scientific research in the United States, including learning about the neurodiversity movement and its impact on establishing ASD research priorities. By explicitly discussing appropriate scientific practices, analyzing the consequences of scientific misconduct and the spread of misinformation, and demonstrating how students can use their voices and their votes to support science funding, we can prepare students to become knowledgeable, empowered, scientifically literate citizens.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"519 ","pages":"Pages 151-158"},"PeriodicalIF":2.5,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142902696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oogenesis involves a novel nuclear envelop remodeling mechanism in Schmidtea mediterranea 地中海Schmidtea mediterranea的卵发生涉及一种新的核膜重塑机制。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-12-26 DOI: 10.1016/j.ydbio.2024.12.018

Longhua Guo , Fengli Guo , Shasha Zhang , An Zeng , Kexi Yi , Melainia McClain , Claus-D. Kuhn , Tari Parmely , Alejandro Sánchez Alvarado

{"title":"Oogenesis involves a novel nuclear envelop remodeling mechanism in Schmidtea mediterranea","authors":"Longhua Guo , Fengli Guo , Shasha Zhang , An Zeng , Kexi Yi , Melainia McClain , Claus-D. Kuhn , Tari Parmely , Alejandro Sánchez Alvarado","doi":"10.1016/j.ydbio.2024.12.018","DOIUrl":"10.1016/j.ydbio.2024.12.018","url":null,"abstract":"<div><div>The cell nuclei of Ophisthokonts, the eukaryotic supergroup defined by fungi and metazoans, is remarkable in the constancy of their double-membraned structure in both somatic and germ cells. Such remarkable structural conservation underscores common and ancient evolutionary origins. Yet, the dynamics of disassembly and reassembly displayed by Ophisthokont nuclei vary extensively. Besides closed mitosis in fungi and open mitosis in some animals, little is known about the evolution of nuclear envelope remodeling dynamics during oogenesis. Here, we uncovered a novel form of nuclear envelope remodeling as oocytes are formed in the flatworm <em>Schmidtea mediterranea</em>. From zygotene to metaphase II, both nuclear envelope (NE) and peripheral endoplasmic reticulum (ER) expand notably in size, likely involving <em>de novo</em> membrane synthesis. 3-D electron microscopy reconstructions demonstrated that the NE transforms itself into numerous double-membraned vesicles similar in membrane architecture to NE doublets in mammalian oocytes after germinal vesicle breakdown. The vesicles are devoid of nuclear pore complexes and DNA, yet are loaded with nuclear proteins, including a planarian homologue of PIWI, a protein essential for the maintenance of stem cells in this and other organisms. Our data contribute a new model to the canonical view of NE dynamics and suggest important roles of NE remodeling in planarian oogenesis.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"520 ","pages":"Pages 13-20"},"PeriodicalIF":2.5,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142892898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0