Developmental biology最新文献_第3页

Semaphorin 3A repulsion directs the caudal projection of pioneer longitudinal axons in the developing chicken brain

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-11-28 DOI: 10.1016/j.ydbio.2024.11.012

Kerry-lyn Riley , Susanne Dietrich , Frank R. Schubert

{"title":"Semaphorin 3A repulsion directs the caudal projection of pioneer longitudinal axons in the developing chicken brain","authors":"Kerry-lyn Riley , Susanne Dietrich , Frank R. Schubert","doi":"10.1016/j.ydbio.2024.11.012","DOIUrl":"10.1016/j.ydbio.2024.11.012","url":null,"abstract":"<div><div>The medial longitudinal fasciculus (MLF) is the first axon tract to develop in the ventral vertebrate brain. It originates in the diencephalon and projects caudally into the spinal cord, pioneering the path for later developing axons. Previous anatomical and expression analyses in the chicken suggested Semaphorin 3 A (Sema3A) as the candidate to repel the amniote MLF from the forebrain. However, studies in the zebrafish implicated a distantly related semaphorin with a role in axon fasciculation, not guidance. Thus, the mechanism accounting for the caudal projection of the MLF remains unclear.</div><div>Here we show that misexpression of Sema3A or grafting of Sema3A-expressing cells into the path of the MLF diverts the axons or blocks their outgrowth in chicken embryos. In vitro, Sema3A exposure resulted in the collapse of MLF growth cones. A dominant-negative approach or siRNA to interfere with the function of the Sema3A receptor Neuropilin1 allowed MLF axons to project rostrally. Together, this suggests that Sema3a repulsion directs the caudal extension of the MLF to pioneer the ventral longitudinal tract.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"518 ","pages":"Pages 77-84"},"PeriodicalIF":2.5,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142757418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A conditional smoothened (smo) allele on an inbred C57BL/6J genetic background has a hypomorphic smo mutant phenotype 在近交系 C57BL/6J 遗传背景上，一个条件性 Smoothened（Smo）等位基因具有低形态 Smo 突变体表型。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-11-26 DOI: 10.1016/j.ydbio.2024.11.010

Scott Houghtaling , Sean K. Gombart , Tzu-Hua Ho , Grace Huang , David R. Beier

引用次数: 0

Disease susceptibility implications of preferential inactivation of the paternal X chromosome in extraembryonic endoderm of the mouse.

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-11-26 DOI: 10.1016/j.ydbio.2024.11.011

Virginia E Papaioannou

引用次数: 0

Spatiotemporal characterization of cyclooxygenase pathway enzymes during vertebrate embryonic development 脊椎动物胚胎发育过程中环氧化酶通路酶的时空表征

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-11-22 DOI: 10.1016/j.ydbio.2024.11.009

Tess A. Leathers, Raneesh Ramarapu, Crystal D. Rogers

{"title":"Spatiotemporal characterization of cyclooxygenase pathway enzymes during vertebrate embryonic development","authors":"Tess A. Leathers, Raneesh Ramarapu, Crystal D. Rogers","doi":"10.1016/j.ydbio.2024.11.009","DOIUrl":"10.1016/j.ydbio.2024.11.009","url":null,"abstract":"<div><div>Vertebrate development is regulated by several complex well-characterized morphogen signaling pathways, transcription factors, and structural proteins, but less is known about the enzymatic pathways that regulate early development. We have identified that factors from the inflammation-mediating cyclooxygenase (COX) signaling pathway are expressed at early stages of development in avian embryos. Using <em>Gallus gallus</em> (chicken) as a research model, we characterized the spatiotemporal expression of a subset of genes and proteins in the COX pathway during early neural development stages. Specifically, here we show expression patterns of COX-1, COX-2, and microsomal prostaglandin E synthase-2 (mPGES-2) as well as the genes encoding these enzymes (<em>PTGS1</em>, <em>PTGS2</em>, and <em>PTGES-2</em>). Unique expression patterns of individual players within the COX pathway suggest that they may play non-canonical/non-traditional roles in the embryo compared to their roles in the adult. Future work should examine the function of the COX pathway in tissue specification and morphogenesis and determine if these expression patterns are conserved across species.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"518 ","pages":"Pages 61-70"},"PeriodicalIF":2.5,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142701340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beyond the heat shock pathway: Heat stress responses in Drosophila development 超越热休克途径：果蝇发育过程中的热应激反应

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-11-16 DOI: 10.1016/j.ydbio.2024.11.003

Julia R. Gibbs, Christian Mei, Zeba Wunderlich

{"title":"Beyond the heat shock pathway: Heat stress responses in Drosophila development","authors":"Julia R. Gibbs, Christian Mei, Zeba Wunderlich","doi":"10.1016/j.ydbio.2024.11.003","DOIUrl":"10.1016/j.ydbio.2024.11.003","url":null,"abstract":"<div><div>Heat stress has broad effects on an organism and is an inevitable part of life. Embryos face a particular challenge when faced with heat stress – the intricate molecular processes that pattern the embryo can all be affected by heat, and the embryo lacks some of the strategies that adults can use to manage or avoid heat stress. We use <em>Drosophila melanogaster</em> as a model, as insects are capable of developing normally under a wide range of temperatures and are exposed to daily temperature swings as they develop. Research has focused on the heat shock pathway and the transcription of heat shock proteins as the main response to heat and heat damage. This review explores embryonic heat responses beyond the heat shock pathway. We examine the effects of heat from a biochemical standpoint, as well as highlighting other mechanisms of heat stress regulation, such as miRNA activity or other signaling pathways. We discuss how different elements of the heat stress response must be coordinated across the embryo to enable development under a wide range of temperatures. Studying heat stress in <em>Drosophila melanogaster</em> can be a powerful lens into how developmental systems ensure robustness to environmental factors.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"518 ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142667342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Approaching ethical issues in the developmental biology classroom 在发育生物学课堂上探讨伦理问题。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-11-16 DOI: 10.1016/j.ydbio.2024.11.008

Heather Ray , Michael Klymkowsky

引用次数: 0

A conserved sequence that sparked the field of evo-devo 一个保守序列引发了进化-变形领域的研究。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-11-15 DOI: 10.1016/j.ydbio.2024.11.005

Leslie Pick, Kristen Au

{"title":"A conserved sequence that sparked the field of evo-devo","authors":"Leslie Pick, Kristen Au","doi":"10.1016/j.ydbio.2024.11.005","DOIUrl":"10.1016/j.ydbio.2024.11.005","url":null,"abstract":"<div><div>The discovery that homeotic genes in <em>Drosophila</em> are conserved and utilized for embryonic development throughout the animal kingdom, including humans, revolutionized the fields of developmental biology and evolutionary developmental biology (evo-devo). In a pair of back-to-back papers published in <em>Cell</em> in 1984, researchers at the Biozentrum in Basel, Switzerland, showed that the homeobox – previously identified as a sequence shared by homeotic genes in <em>Drosophila</em> – was also present in the genome of diverse animals. The first paper (McGinnis et al., 1984a) showed that genomes of both invertebrates and vertebrates contain sequences that cross-hybridized with <em>Drosophila</em> homeobox probes. The second paper (Carrasco et al., 1984) identified a cross-hybridizing sequence in the model system <em>Xenopus laevis</em>. They then isolated the first vertebrate homeobox-containing gene by cloning and sequencing of the corresponding genomic region. Finally, they showed that this gene (<em>AC1</em>, later renamed <em>HoxC6</em>) was expressed during embryonic development, the first evidence that developmentally expressed <em>Drosophila</em> genes could be used to isolate regulators of vertebrate embryonic development. These findings led to a flurry of activity in the evo-devo field, initially focused on isolating <em>Hox</em> genes across diverse species, and then expanding to isolation of other gene families based on <em>Drosophila</em> orthologs, an approach that continues today. This led to the notion of a conserved genetic toolkit for embryonic development, currently accepted, but unexpected at the time of its discovery. We attempt to provide some context for the sea-change in thinking that these discoveries brought about by referring to Jean Piaget's theories about the sequential acquisition of scientific knowledge.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"518 ","pages":"Pages 1-7"},"PeriodicalIF":2.5,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142643757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maternal and zygotic contributions to H3K4me1 chromatin marking during germ layer formation 胚层形成过程中母体和子代对H3K4me1染色质标记的贡献

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-11-15 DOI: 10.1016/j.ydbio.2024.11.006

Kitt D. Paraiso , Ira L. Blitz , Ken W.Y. Cho

{"title":"Maternal and zygotic contributions to H3K4me1 chromatin marking during germ layer formation","authors":"Kitt D. Paraiso , Ira L. Blitz , Ken W.Y. Cho","doi":"10.1016/j.ydbio.2024.11.006","DOIUrl":"10.1016/j.ydbio.2024.11.006","url":null,"abstract":"<div><div>An early step in triploblastic embryo differentiation is the formation of the three germ layers. Maternal pioneer transcription factors (TFs) bind to embryonic enhancers before zygotic genome activation, initiating germ layer specification. While maternal TFs' role in establishing epigenetic marks is known, how early pluripotent cells gain spatially restricted epigenetic identities remains unclear. We show that by the early gastrula stage, H3K4me1-marked regions become distinct in each germ layer, with certain chromatin regions forming high density H3K4me1 marked regions (HDRs). Genes associated with these HDRs are more robustly expressed compared to those associated with low density H3K4me1 marked regions (LDRs) in the genome. This process is driven by the sequential actions of maternal and zygotic factors. Knockdown of key maternal endodermal TFs (Otx1, Vegt and Foxh1) leads to a loss of endodermal H3K4me1 marks in endoderm, with a concurrent emergence of ectodermal and mesodermal marks, indicating a shift in chromatin state. This work highlights the importance of coordinated activities of maternal and zygotic TFs in defining the regionally-resolved and dynamic process of chromatin modification conferred by H3K4me1 in the early <em>Xenopus</em> embryo.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"518 ","pages":"Pages 8-19"},"PeriodicalIF":2.5,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142643762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The establishment of the anther somatic niche with single-cell sequencing 利用单细胞测序建立花药体细胞龛。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-11-14 DOI: 10.1016/j.ydbio.2024.11.004

D. Blaine Marchant , Virginia Walbot

{"title":"The establishment of the anther somatic niche with single-cell sequencing","authors":"D. Blaine Marchant , Virginia Walbot","doi":"10.1016/j.ydbio.2024.11.004","DOIUrl":"10.1016/j.ydbio.2024.11.004","url":null,"abstract":"<div><div>The anther is the developmental housing of pollen and therefore the male gametes of flowering plants. The meiotic cells from which pollen are derived must differentiate <em>de novo</em> from somatic anther cells and synchronously develop with the rest of the anther. Anthropogenic control over another development has become crucial for global agriculture so as to maintain inbred lines and generate hybrid seeds of many crops. Understanding the genes that underlie the proper differentiation, developmental landmarks, and functions of each anther cell type is thus fundamental to both basic and applied plant sciences. We investigated the development of the somatic niche of the maize (<em>Zea mays</em>) anther using single-cell RNA-seq (scRNA-seq). Extensive background knowledge on the birth then pace and pattern of cell division of the maize anther cell types and published examples of cell-type gene expression from <em>in situ</em> hybridization allowed us to identify the primary cell types within the anther lobe, as well as the connective cells between the four lobes. We established the developmental trajectories of somatic cell types from pre-meiosis to post-meiosis, identified putative marker genes for the somatic cell types that previously lacked any known specific functions, and addressed the possibility that tapetal cells sequentially differentiate. This comprehensive scRNA-seq dataset of the somatic niche of the maize anther will serve as a baseline for future analyses investigating male-sterile genotypes and the impact of environmental conditions on male fertility in flowering plants.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"518 ","pages":"Pages 37-47"},"PeriodicalIF":2.5,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142638665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The human gestational sac as a choriovitelline placenta during early pregnancy; the secondary yolk sac and organoid models 人类妊娠囊作为绒毛膜胎盘在妊娠早期的作用；次级卵黄囊和类器官模型。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-11-14 DOI: 10.1016/j.ydbio.2024.11.007

Graham J. Burton , Eric Jauniaux , Tereza Cindrova-Davies , Margherita Y. Turco

{"title":"The human gestational sac as a choriovitelline placenta during early pregnancy; the secondary yolk sac and organoid models","authors":"Graham J. Burton , Eric Jauniaux , Tereza Cindrova-Davies , Margherita Y. Turco","doi":"10.1016/j.ydbio.2024.11.007","DOIUrl":"10.1016/j.ydbio.2024.11.007","url":null,"abstract":"<div><div>The yolk sac is phylogenetically the oldest of the extra-embryonic membranes and plays important roles in nutrient transfer during early pregnancy in many species. In the human this function is considered largely vestigial, in part because the secondary yolk sac never makes contact with the inner surface of the chorionic sac. Instead, it is separated from the chorion by the fluid-filled extra-embryonic coelom and attached to the developing embryo by a relatively long vitelline duct. The coelomic fluid is, however, rich in nutrients and key co-factors, including folic acid and anti-oxidants, derived from maternal plasma and the endometrial glands. Bulk sequencing has recently revealed the presence of transcripts encoding numerous transporter proteins for these ligands. Mounting evidence suggests the human secondary yolk sac plays a pivotal role in the transfer of histotrophic nutrition during the critical phase of organogenesis but also of chemicals such as medical drugs and cotinine. We therefore propose that the early placental villi, coelomic cavity and yolk sac combine to function physiologically as a choriovitelline placenta during the first weeks of pregnancy. We have derived organoids from the mouse yolk sac as proof-of-principle of a model system that could be used to answer many questions concerning the functional capacity of the human yolk sac as a maternal-fetal exchange interface during the first trimester of pregnancy.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"518 ","pages":"Pages 28-36"},"PeriodicalIF":2.5,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142643808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0