Developmental biology最新文献_第4页

Introduction to “Research that Transformed Developmental Biology” 改变发育生物学的研究 "简介。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-12-27 DOI: 10.1016/j.ydbio.2024.12.011

Steven L. Klein Ph.D.

引用次数: 0

Teaching students to effectively evaluate scientific evidence and advocate for research in the context of autism spectrum disorder and the neurodiversity movement 在自闭症谱系障碍和神经多样性运动的背景下，教学生有效地评估科学证据并倡导研究。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-12-27 DOI: 10.1016/j.ydbio.2024.12.015

Bridget T. Jacques-Fricke

{"title":"Teaching students to effectively evaluate scientific evidence and advocate for research in the context of autism spectrum disorder and the neurodiversity movement","authors":"Bridget T. Jacques-Fricke","doi":"10.1016/j.ydbio.2024.12.015","DOIUrl":"10.1016/j.ydbio.2024.12.015","url":null,"abstract":"<div><div>Connecting socially relevant topics with biological content can boost student engagement and comprehension. Autism Spectrum Disorder (ASD) is an increasingly prevalent diagnosis with a number of intersecting topic areas between developmental biology and social justice. Here I describe two exercises that I developed to engage students in learning opportunities that link scientific process learning goals with real-world applications. First, students examine scientific research practices and work on connecting scientific evidence with conclusions by evaluating the retracted 1998 article by Andrew Wakefield that falsely linked the measles, mumps and rubella vaccination with the development of ASD. Second, students participate in a role-playing exercise to learn about the multiple viewpoints and perspectives that are involved in determining funding levels for scientific research in the United States, including learning about the neurodiversity movement and its impact on establishing ASD research priorities. By explicitly discussing appropriate scientific practices, analyzing the consequences of scientific misconduct and the spread of misinformation, and demonstrating how students can use their voices and their votes to support science funding, we can prepare students to become knowledgeable, empowered, scientifically literate citizens.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"519 ","pages":"Pages 151-158"},"PeriodicalIF":2.5,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142902696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oogenesis involves a novel nuclear envelop remodeling mechanism in Schmidtea mediterranea 地中海Schmidtea mediterranea的卵发生涉及一种新的核膜重塑机制。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-12-26 DOI: 10.1016/j.ydbio.2024.12.018

Longhua Guo , Fengli Guo , Shasha Zhang , An Zeng , Kexi Yi , Melainia McClain , Claus-D. Kuhn , Tari Parmely , Alejandro Sánchez Alvarado

{"title":"Oogenesis involves a novel nuclear envelop remodeling mechanism in Schmidtea mediterranea","authors":"Longhua Guo , Fengli Guo , Shasha Zhang , An Zeng , Kexi Yi , Melainia McClain , Claus-D. Kuhn , Tari Parmely , Alejandro Sánchez Alvarado","doi":"10.1016/j.ydbio.2024.12.018","DOIUrl":"10.1016/j.ydbio.2024.12.018","url":null,"abstract":"<div><div>The cell nuclei of Ophisthokonts, the eukaryotic supergroup defined by fungi and metazoans, is remarkable in the constancy of their double-membraned structure in both somatic and germ cells. Such remarkable structural conservation underscores common and ancient evolutionary origins. Yet, the dynamics of disassembly and reassembly displayed by Ophisthokont nuclei vary extensively. Besides closed mitosis in fungi and open mitosis in some animals, little is known about the evolution of nuclear envelope remodeling dynamics during oogenesis. Here, we uncovered a novel form of nuclear envelope remodeling as oocytes are formed in the flatworm <em>Schmidtea mediterranea</em>. From zygotene to metaphase II, both nuclear envelope (NE) and peripheral endoplasmic reticulum (ER) expand notably in size, likely involving <em>de novo</em> membrane synthesis. 3-D electron microscopy reconstructions demonstrated that the NE transforms itself into numerous double-membraned vesicles similar in membrane architecture to NE doublets in mammalian oocytes after germinal vesicle breakdown. The vesicles are devoid of nuclear pore complexes and DNA, yet are loaded with nuclear proteins, including a planarian homologue of PIWI, a protein essential for the maintenance of stem cells in this and other organisms. Our data contribute a new model to the canonical view of NE dynamics and suggest important roles of NE remodeling in planarian oogenesis.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"520 ","pages":"Pages 13-20"},"PeriodicalIF":2.5,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142892898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A chitin-binding domain-containing gene is essential for shell development in the mollusc Tritia 一种含有几丁质结合结构域的基因对软体动物的壳发育至关重要。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-12-24 DOI: 10.1016/j.ydbio.2024.12.016

T. Kim Dao, Kailey Ferger , J. David Lambert

引用次数: 0

Post-eclosion growth in the Drosophila ejaculatory duct is driven by Juvenile hormone signaling and is essential for male fertility 果蝇射精管的羽化后生长是由幼年激素信号驱动的，对雄性生殖能力至关重要。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-12-22 DOI: 10.1016/j.ydbio.2024.12.010

Navyashree A. Ramesh, Allison M. Box, Laura A. Buttitta

{"title":"Post-eclosion growth in the Drosophila ejaculatory duct is driven by Juvenile hormone signaling and is essential for male fertility","authors":"Navyashree A. Ramesh, Allison M. Box, Laura A. Buttitta","doi":"10.1016/j.ydbio.2024.12.010","DOIUrl":"10.1016/j.ydbio.2024.12.010","url":null,"abstract":"<div><div>The <em>Drosophila</em> Ejaculatory duct (ED) is a secretory tissue of the somatic male reproductive system. The ED is involved in the secretion of seminal fluid components and ED-specific antimicrobial peptides that aid in fertility and the female post-mating response. The ED is composed of secretory epithelial cells surrounded by a layer of innervated contractile muscle. The ED grows in young adult males during the first 24 h post-eclosion, but the cell cycle status of the ED secretory cells and the role of post-eclosion ED growth have been unexplored. Here, we show that secretory cells of the adult <em>Drosophila</em> ED undergo variant cell cycles lacking mitosis called the endocycle, that lead to an increase in the cell and organ size of the ED post eclosion. The cells largely exit the endocycle by day 3 of adulthood, when the growth of the ED ceases, resulting in a tissue containing cells of ploidies ranging from 8C to 32C. The size of the ED directly correlates with the ploidy of the secretory cells, with additional ectopic endocycles increasing organ size. When endoreplication is compromised in ED secretory cells, it leads to reduced organ size, reduced protein synthesis and compromised fertility. We provide evidence that the growth and endocycling in the young adult male ED is dependent on Juvenile hormone (JH) signaling and we suggest that hormone-induced early adult endocycling is required for optimal fertility and function of the ED tissue. We propose to use the ED as a post-mitotic tissue model to study the role of polyploidy in regulating secretory tissue growth and function.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"519 ","pages":"Pages 122-141"},"PeriodicalIF":2.5,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The transcriptional landscape of the developing chick trigeminal ganglion 发育中的小鸡三叉神经节的转录景观。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-12-22 DOI: 10.1016/j.ydbio.2024.12.013

Carrie E. Leonard , Alec McIntosh , Johena Sanyal , Lisa A. Taneyhill

{"title":"The transcriptional landscape of the developing chick trigeminal ganglion","authors":"Carrie E. Leonard , Alec McIntosh , Johena Sanyal , Lisa A. Taneyhill","doi":"10.1016/j.ydbio.2024.12.013","DOIUrl":"10.1016/j.ydbio.2024.12.013","url":null,"abstract":"<div><div>The trigeminal ganglion is a critical structure in the peripheral nervous system, responsible for transmitting sensations of touch, pain, and temperature from craniofacial regions to the brain. Trigeminal ganglion development depends upon intrinsic cellular programming as well as extrinsic signals exchanged by diverse cell populations. With its complex anatomy and dual cellular origin from cranial placodes and neural crest cells, the trigeminal ganglion offers a rich context for examining diverse biological processes, including cell migration, fate determination, adhesion, and axon guidance. Avian models have, so far, enabled key insights into craniofacial and peripheral nervous system development. Yet the molecular mechanisms driving trigeminal ganglion formation and subsequent nerve growth remain elusive. In this study, we performed RNA-sequencing at multiple stages of chick trigeminal ganglion development and generated a novel transcriptomic dataset that has been curated to illustrate temporally dynamic gene expression patterns. This publicly available resource identifies major pathways involved in trigeminal gangliogenesis, particularly with respect to the condensation and maturation of placode-derived neurons, thus inviting new lines of research into the essential processes governing trigeminal ganglion development.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"520 ","pages":"Pages 108-116"},"PeriodicalIF":2.5,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analysis of neural crest development in the chick and mouse 鸡与小鼠神经嵴发育的比较分析。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-12-21 DOI: 10.1016/j.ydbio.2024.12.014

J.A. Morrison , I. Pushel , R. McLennan , M.C. McKinney , M.M. Gogol , A. Scott , R. Krumlauf , P.M. Kulesa

{"title":"Comparative analysis of neural crest development in the chick and mouse","authors":"J.A. Morrison , I. Pushel , R. McLennan , M.C. McKinney , M.M. Gogol , A. Scott , R. Krumlauf , P.M. Kulesa","doi":"10.1016/j.ydbio.2024.12.014","DOIUrl":"10.1016/j.ydbio.2024.12.014","url":null,"abstract":"<div><div>A core framework of the gene regulatory network (GRN) governing neural crest (NC) cell development has been generated by integrating separate inputs from diverse model organisms rather than direct comparison. This has limited insights into the diversity of genes in the NC cell GRN and extent of conservation of newly identified transcriptional signatures in cell differentiation and invasion. Here, we address this by leveraging the strengths and accessibility of the avian embryo to precise developmental staging by egg incubation and use an integrated analysis of chick (HH13) and mouse (E9.5) embryo tissue samples collected during NC cell migration into pharyngeal arches 1–2 (PA1 and PA2). We successfully identify a cluster of NC cells containing both mouse and chick cells that share expression of <em>Lmo4</em>, <em>Tfap2B</em>, <em>Sox10</em>, and <em>Twist1</em>, and distinct genes that lack known conserved roles in NC. Importantly, we discovered a cluster of cells exhibiting a conserved transcriptional signature associated with the NC cell migratory wavefront in both mouse and chick, including KAZALD1, BAMBI, DES, and GPC3. We confirm their expression is restricted to leader mouse NCs by multiplexed FISH. Together, these data offer novel insights into the transcriptional programs that underlie NC cell migration and establish the foundation for future comparative functional analyses.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"519 ","pages":"Pages 142-149"},"PeriodicalIF":2.5,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142880679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mesenchymal cell contractility regulates villus morphogenesis and intestinal architecture 间充质细胞收缩调节绒毛形态发生和肠道结构。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-12-19 DOI: 10.1016/j.ydbio.2024.12.012

Taylor D. Hinnant , Caroline Joo , Terry Lechler

{"title":"Mesenchymal cell contractility regulates villus morphogenesis and intestinal architecture","authors":"Taylor D. Hinnant , Caroline Joo , Terry Lechler","doi":"10.1016/j.ydbio.2024.12.012","DOIUrl":"10.1016/j.ydbio.2024.12.012","url":null,"abstract":"<div><div>The large absorptive surface area of the small intestine is imparted by finger-like projections called villi. Villi formation is instructed by stromal-derived clusters of cells which have been proposed to induce epithelial bending through actomyosin contraction. Their functions in the elongation of villi have not been studied. Here, we explored the function of mesenchymal contractility at later stages of villus morphogenesis. We induced contractility specifically in the mesenchyme of the developing intestine through inducible overexpression of the RhoA GTPase activator Arhgef11. This resulted in overgrowth of the clusters through a YAP-mediated increase in cell proliferation. While epithelial bending occurred in the presence of contractile clusters, the resulting villi had architectural defects, being shorter and wider than controls. These villi also had defects in epithelial organization and the establishment of nutrient-absorbing enterocytes. While ectopic activation of YAP resulted in similar cluster overgrowth and wider villi, it did not affect villus elongation or enterocyte differentiation, demonstrating roles for contractility in addition to proliferation. We find that the specific contractility-induced effects were dependent upon cluster interaction with the extracellular matrix. Together, these data demonstrate effects of contractility on villus morphogenesis and distinguish separable roles for proliferation and contractility in controlling intestinal architecture.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"519 ","pages":"Pages 96-105"},"PeriodicalIF":2.5,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142871678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The chorioallantoic membrane (CAM) model: From its origins in developmental biology to its role in cancer research 绒毛膜尿囊膜（CAM）模型：从发育生物学起源到在癌症研究中的作用。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-12-16 DOI: 10.1016/j.ydbio.2024.12.007

María Jimena Mosna , Federico J. Garde , Marcelo G. Stinson , Candela D. Pastore , Abel L. Carcagno

{"title":"The chorioallantoic membrane (CAM) model: From its origins in developmental biology to its role in cancer research","authors":"María Jimena Mosna , Federico J. Garde , Marcelo G. Stinson , Candela D. Pastore , Abel L. Carcagno","doi":"10.1016/j.ydbio.2024.12.007","DOIUrl":"10.1016/j.ydbio.2024.12.007","url":null,"abstract":"<div><div>Over the past century, the chick embryo model, historically employed for research in developmental biology, has become a valuable tool for cancer research. The characteristics of the chick chorioallantoic membrane (CAM) make it a convenient model for the study of cancer, leading to the establishment of the CAM assay as an alternative to traditional <em>in vivo</em> cancer models. In this review we will explore the characteristics of the CAM that make it suitable for cancer research, as well as its consolidation as a versatile platform in this field. We will put particular emphasis on describing the key features that make this model an important asset for studying the hallmarks of cancer and for testing a wide variety of therapeutic strategies for its treatment, and which make it a suitable host for patient-derived xenografts (PDX). Additionally, we will examine the wide spectrum of methodological approaches available to study these subjects, highlighting some innovative cases. Finally, we will discuss the advantages and disadvantages of the chick CAM as a model for cancer research and how we can improve this model to its full potential.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"519 ","pages":"Pages 79-95"},"PeriodicalIF":2.5,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142853047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative proteomics of regenerating and non-regenerating spinal cords in Xenopus 非洲爪蟾再生和非再生脊髓的定量蛋白质组学研究。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-12-16 DOI: 10.1016/j.ydbio.2024.12.009

Aniket Kshirsagar , Rachel Ronan , Ana Lúcia Rebelo , Siobhan McMahon , Abhay Pandit , Gerhard Schlosser

{"title":"Quantitative proteomics of regenerating and non-regenerating spinal cords in Xenopus","authors":"Aniket Kshirsagar , Rachel Ronan , Ana Lúcia Rebelo , Siobhan McMahon , Abhay Pandit , Gerhard Schlosser","doi":"10.1016/j.ydbio.2024.12.009","DOIUrl":"10.1016/j.ydbio.2024.12.009","url":null,"abstract":"<div><div>Spinal cord injury in humans is a life-changing condition with no effective treatment. However, many non-mammalian vertebrates can fully regenerate their spinal cord after injury. Frogs such as <em>Xenopus</em> can regenerate the spinal cord at larval stages, but lose this capacity at metamorphosis. This makes them ideal models to elucidate molecular pathways underlying regenerative capacity by comparing responses to spinal cord injury in regenerative (R) and non-regenerative (NR) stages of the same species. Here we use quantitative proteomics with Isobaric Tags for Relative and Absolute Quantification (iTRAQ) followed by Ingenuity Pathway Analysis (IPA) to identify functions and pathways that were differentially regulated after spinal cord injury between R and NR stages in <em>Xenopus laevis</em>. We find that many embryonic pathways of neuronal development are re-activated following SCI at the R but not at the NR stage. This is accompanied by the upregulation of regulatory proteins controlling transcription and translation at the R stage, but their downregulation at the NR stage. Conversely, lipid hydrolysis and uptake as well as mitochondrial oxidative phosphorylation is downregulated at the R, but upregulated at the NR stage. Taken together this suggests that dysregulation of lipid homeostasis and augmentation of oxidative stress play a key role in the loss of regenerative capacity of the spinal cord after metamorphosis. In identifying new factors regulating regenerative capacity in the vertebrate spinal cord, our findings suggest new potential therapeutic targets for promoting neural repair in the injured adult mammalian spinal cord.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"519 ","pages":"Pages 65-78"},"PeriodicalIF":2.5,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142852974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0