Developmental biology最新文献_第4页

Novel features of Drosophila hematopoiesis uncovered by long-term live imaging 通过长期实时成像揭示果蝇造血的新特征。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-11-12 DOI: 10.1016/j.ydbio.2024.10.004

Kevin Y.L. Ho , Annie Y.J. Ou , Nicholas Samuelson , Guy Tanentzapf

{"title":"Novel features of Drosophila hematopoiesis uncovered by long-term live imaging","authors":"Kevin Y.L. Ho , Annie Y.J. Ou , Nicholas Samuelson , Guy Tanentzapf","doi":"10.1016/j.ydbio.2024.10.004","DOIUrl":"10.1016/j.ydbio.2024.10.004","url":null,"abstract":"<div><div>Stem cells are subject to continuous regulation to ensure that the correct balance between stem cell differentiation and self-renewal is maintained. The dynamic and ongoing nature of stem cell regulation, as well as the complex signaling microenvironment in which stem cells are typically found, means that studying them in their endogenous environment in real time has multiple advantages over static fixed-sample approaches. We recently described a method for long-term, <em>ex-vivo</em>, live imaging of the blood progenitors in the <em>Drosophila</em> larval hematopoietic organ, the Lymph Gland (LG). This methodology has allowed us to analyze multiple aspects of fly hematopoiesis, in real time, in a manner that could not be carried out previously. Here, we describe novel insights derived from our quantitative live imaging approach. These insights include: the identification of extensive filopodia in the progenitors and description of their morphology and dynamics; visualization and quantitative analysis of JAK/STAT signaling in progenitors by the simultaneous tracking of thousands of vesicles containing internalized Domeless receptors; quantitative analysis of the location, morphology, and dynamics of mitochondria in blood progenitors; long-term tracking of patterns of cell division and migration of mature blood cell in the LG; long-term tracking of multiple cell behaviors in the distal committed progenitors; analysis of Ca<sup>2+</sup> signaling of blood progenitors in the secondary lobes of the LG. Together, these observations illustrate the power of imaging fly hematopoiesis in real time and identify many previously undescribed processes and behaviors in the LG that are likely to play important roles in the regulation of progenitor differentiation and self-renewal.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"517 ","pages":"Pages 286-300"},"PeriodicalIF":2.5,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142615146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modifying membrane potential synchronously controls the somite's formation periodicity and growth 同步调节膜电位可控制体节的形成周期和生长。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-11-08 DOI: 10.1016/j.ydbio.2024.11.002

Manohara Mahadeva, Sebastian Niestępski, Magdalena Kowacz

{"title":"Modifying membrane potential synchronously controls the somite's formation periodicity and growth","authors":"Manohara Mahadeva, Sebastian Niestępski, Magdalena Kowacz","doi":"10.1016/j.ydbio.2024.11.002","DOIUrl":"10.1016/j.ydbio.2024.11.002","url":null,"abstract":"<div><div>Coordination between periodicity of somite formation and somite growth is crucial for regular body pattern formation during somitogenesis. Yet, the specific mechanism that links the two processes remains unclear. Using chick embryos, we demonstrate that both temporal and spatial features can be simultaneously controlled by membrane potential (V<sub>m</sub>) of somite-forming cells. Our findings show that somites hyperpolarize as they mature, displaying step-like changes in V<sub>m</sub> observed between specific groups of somites, reflecting the reported onset of biochemical and structural changes within them. We modify V<sub>m</sub> by changing chemical compositions of the microenvironment of the embryo. Alteration of V<sub>m</sub> sets a new pace of somite formation (cell migration and self-assembly) and its concurrent growth (cell proliferation) without disturbing the somite's regular aspect ratio. Our results therefore suggest that V<sub>m</sub> has the ability to orchestrate cell proliferation, migration and self-assembly - processes that are hallmarks of embryogenesis, tumorigenesis and tissue regeneration.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"517 ","pages":"Pages 317-326"},"PeriodicalIF":2.5,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142615215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

R391 human dominant mutation does not affect TubB4b localization and sensory hair cells structure in zebrafish inner ear and lateral line R391 人类显性突变不会影响斑马鱼内耳和侧线中 TubB4b 的定位和感觉毛细胞的结构。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-11-07 DOI: 10.1016/j.ydbio.2024.11.001

Wiam Smaili , Camille Pezet , Sandrine Marlin , Sylvain Ernest

{"title":"R391 human dominant mutation does not affect TubB4b localization and sensory hair cells structure in zebrafish inner ear and lateral line","authors":"Wiam Smaili , Camille Pezet , Sandrine Marlin , Sylvain Ernest","doi":"10.1016/j.ydbio.2024.11.001","DOIUrl":"10.1016/j.ydbio.2024.11.001","url":null,"abstract":"<div><div>Heterozygous R391 <em>TUBB4B</em> pathogenic variations are responsible for an association of hearing loss and retinal dystrophy in human. With the goal of understanding the functions of TuBB4b and the pathogenic role of R391 variations, we characterized <em>tubB4B</em> in zebrafish and identified the gene regulatory elements necessary and sufficient for expression of TubB4b as in endogenous tissues. Using knock-out and transgenic approaches, we determined that R391 mutations impair neither localization of TubB4B within sensory hair cells (SHC) nor their structure, but induced to a small decrease in SHC number from anterior crista. Expression of R391 mutations in sensory hair cells has no effect on zebrafish audition, suggesting a different equilibrium between various tubulin isotypes in zebrafish possibly due to compensatory mechanisms. The careful expression analysis and transgenic tools generated in this study could help understand how recently described pathogenic variants lead to more severe clinical forms of TUBB4B-related diseases.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"517 ","pages":"Pages 301-316"},"PeriodicalIF":2.5,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142602701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Off to a good start: The importance of the placental exchange surface – Lessons from the mouse 一个良好的开端胎盘交换面的重要性--小鼠的启示。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-11-02 DOI: 10.1016/j.ydbio.2024.10.009

Noura Ballasy , Ifeoluwa Apantaku , Wendy Dean , Myriam Hemberger

{"title":"Off to a good start: The importance of the placental exchange surface – Lessons from the mouse","authors":"Noura Ballasy , Ifeoluwa Apantaku , Wendy Dean , Myriam Hemberger","doi":"10.1016/j.ydbio.2024.10.009","DOIUrl":"10.1016/j.ydbio.2024.10.009","url":null,"abstract":"<div><div>The role of the chorio-allantoic placenta as the critical nutrient- and oxygen-supplying organ to nourish the demands of the fetus has been well recognized. This function relies on the successful establishment of the placental feto-maternal exchange unit, or interhaemal barrier, across which all nutrients as well as waste products must pass to cross from the maternal to the fetal blood circulation, or vice versa, respectively. As a consequence, defects in the establishment of this elaborate interface lead to fetal growth retardation or even embryonic lethality, depending on the severity of the defect. Beyond this essential role, however, it has also emerged that the functionality of the feto-maternal interface dictates the proper development of specific embryonic organs, with tightest links observed to the formation of the heart. In this article, we build on the foundational strength of the mouse as experimental model in which the placental causality of embryonic defects can be genetically proven. We discuss in detail the formation of the interhaemal barrier that makes up the labyrinth layer of the murine placenta, including insights into drivers of its formation and the interdependence of the cell types that make up this essential interface, from <em>in vivo</em> and <em>in vitro</em> data using mouse trophoblast stem cells. We highlight mouse genetic tools that enable the elucidation of cause-effect relationships between defects driven by either the trophoblast cells of the placenta or by embryonic cell types. We specifically emphasize gene knockouts for which a placental causality of embryonic heart defects has been demonstrated. This in-depth perspective provides much-needed insights while highlighting remaining gaps in knowledge that are essential for gaining a better understanding of the multi-facetted roles of the placenta in setting us up for a healthy start in life well beyond nutritional support alone.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"517 ","pages":"Pages 248-264"},"PeriodicalIF":2.5,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142567747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Pax transcription factor EGL-38 links EGFR signaling to assembly of a cell type-specific apical extracellular matrix in the Caenorhabditis elegans vulva Pax转录因子EGL-38将表皮生长因子受体信号传导与草履虫外阴部细胞类型特异性顶端细胞外基质的组装联系起来。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-11-01 DOI: 10.1016/j.ydbio.2024.10.008

Helen F. Schmidt, Chelsea B. Darwin, Meera V. Sundaram

{"title":"The Pax transcription factor EGL-38 links EGFR signaling to assembly of a cell type-specific apical extracellular matrix in the Caenorhabditis elegans vulva","authors":"Helen F. Schmidt, Chelsea B. Darwin, Meera V. Sundaram","doi":"10.1016/j.ydbio.2024.10.008","DOIUrl":"10.1016/j.ydbio.2024.10.008","url":null,"abstract":"<div><div>The surface of epithelial tissues is covered by an apical extracellular matrix (aECM). The aECMs of different tissues have distinct compositions to serve distinct functions, yet how a particular cell type assembles the proper aECM is not well understood. We used the cell type-specific matrix of the <em>C. elegans</em> vulva to investigate the connection between cell identity and matrix assembly. The vulva is an epithelial tube composed of seven cell types descending from EGFR/Ras-dependent (1°) and Notch-dependent (2°) lineages. Vulva aECM contains multiple Zona Pellucida domain (ZP) proteins, which are a common component of aECMs across life. ZP proteins LET-653 and CUTL-18 assemble on 1° cell surfaces, while NOAH-1 assembles on a subset of 2° surfaces. All three ZP genes are broadly transcribed, indicating that cell type-specific ZP assembly must be determined by features of the destination cell surface. The paired box (Pax) transcription factor EGL-38 promotes assembly of 1° matrix and prevents inappropriate assembly of 2° matrix, suggesting that EGL-38 promotes expression of one or more ZP matrix organizers. Our results connect the known signaling pathways and various downstream effectors to EGL-38/Pax expression and the ZP matrix component of vulva cell fate execution. We propose that dedicated transcriptional networks may contribute to cell-appropriate assembly of aECM in many epithelial organs.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"517 ","pages":"Pages 265-277"},"PeriodicalIF":2.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142567751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Blastocoel expansion and AMOT degradation cooperatively promote YAP nuclear localization during epiblast formation 在上胚层形成过程中，胚盘扩张和 AMOT 降解共同促进了 YAP 的核定位。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-10-30 DOI: 10.1016/j.ydbio.2024.10.007

Hinako Maeda (前田日向子), Hiroshi Sasaki (佐々木洋)

{"title":"Blastocoel expansion and AMOT degradation cooperatively promote YAP nuclear localization during epiblast formation","authors":"Hinako Maeda (前田日向子), Hiroshi Sasaki (佐々木洋)","doi":"10.1016/j.ydbio.2024.10.007","DOIUrl":"10.1016/j.ydbio.2024.10.007","url":null,"abstract":"<div><div>The epiblast is a pluripotent cell population formed in the late blastocyst stage of preimplantation embryos. During the process of epiblast formation from the inner cell mass (ICM) of the early blastocyst, activation of the Hippo pathway transcription factor TEAD by the nuclear translocation of the coactivator protein YAP is required for the robust expression of pluripotency factors. However, the mechanisms that alter YAP localization during epiblast formation remain unknown. Here, we reveal two such mechanisms. Expansion of the blastocoel promotes nuclear YAP localization by increasing cytoplasmic F-actin and reducing YAP phosphorylation. Additionally, cell differentiation regulates YAP. Expression of the junctional Hippo component, AMOT, gradually decreases during epiblast formation through a tankyrase-mediated degradation. SOX2 expression in the ICM is necessary for the reduction of AMOT and YAP phosphorylation. These two mechanisms function in parallel. Thus, the blastocoel–F-actin and SOX2–AMOT axes cooperatively suppress YAP phosphorylation and promote YAP nuclear localization during epiblast formation. The cooperation of these two distinct mechanisms likely contributes to the robustness of epiblast cell differentiation.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"517 ","pages":"Pages 234-247"},"PeriodicalIF":2.5,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142564164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preimplantation trophectoderm: A ‘quick-fix’ protector for embryo survival? 植入前滋养外胚层：胚胎存活的 "速效 "保护器？

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-10-30 DOI: 10.1016/j.ydbio.2024.10.006

Tom P. Fleming

引用次数: 0

Development of ectodermal and endodermal taste buds 外胚层和内胚层味蕾的发育

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-10-30 DOI: 10.1016/j.ydbio.2024.10.005

Linda A. Barlow

引用次数: 0

Sex specific gene expression is present prior to metamorphosis in the sea urchin 海胆在变态之前就存在性别特异性基因表达。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-10-18 DOI: 10.1016/j.ydbio.2024.10.003

Cosmo Pieplow, Aidan Furze, Pauline Gregory, Nathalie Oulhen, Gary M. Wessel

{"title":"Sex specific gene expression is present prior to metamorphosis in the sea urchin","authors":"Cosmo Pieplow, Aidan Furze, Pauline Gregory, Nathalie Oulhen, Gary M. Wessel","doi":"10.1016/j.ydbio.2024.10.003","DOIUrl":"10.1016/j.ydbio.2024.10.003","url":null,"abstract":"<div><div>A profound collaboration between the germline and somatic cells of an organism is the creation of a functional gonad. Here we establish a foundation for studying molecular gonadogenesis in the sea urchin by use of RNA-seq, quantitative mRNA measurements, and in-situ hybridizations throughout the life cycle of the variegated sea urchin, <em>Lytechinus variegatus (Lv)</em>. We found through three distinct analyses that the ovary and testis of this echinoderm expresses unique transcripts involved in gametogenesis, and also discovered uncharacterized gene products unique to each gonad. We further developed a pipeline integrating timepoint RNA-seq data throughout development to identify hallmark gene expression in gonads. We found that meiotic and candidate genes involved in sex determination are first expressed surprisingly early during larval growth, and well before metamorphosis. We further discovered that individual larvae express varying amounts of male- or female-hallmarks before metamorphosis, including germline, oocyte, sperm, and meiotic related genes. These distinct male- or female-gonad gene profiles may indicate the onset of, and commitment to, development of a bipotential gonad primordium, and may include metabolic differences, supported by the observation that transcripts involved in glycolysis are highly enriched in the ovary compared to the testis. Together these data support a hypothesis that sex determination is initiated prior to metamorphosis in the sea urchin and that the many uncharacterized genes unique to each gonad type characterized herein may reveal unique pathways and mechanisms in echinoderm reproduction.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"517 ","pages":"Pages 217-233"},"PeriodicalIF":2.5,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142460121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surrounding tissue morphogenesis with disrupted posterior midgut invagination during Drosophila gastrulation 果蝇胃形成过程中后部中肠内陷的周围组织形态发生紊乱

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-10-09 DOI: 10.1016/j.ydbio.2024.10.001

Sandra Sabbagh, Tony J.C. Harris

{"title":"Surrounding tissue morphogenesis with disrupted posterior midgut invagination during Drosophila gastrulation","authors":"Sandra Sabbagh, Tony J.C. Harris","doi":"10.1016/j.ydbio.2024.10.001","DOIUrl":"10.1016/j.ydbio.2024.10.001","url":null,"abstract":"<div><div>Gastrulation involves multiple, physically-coupled tissue rearrangements. During Drosophila gastrulation, posterior midgut (PMG) invagination promotes both germband extension and hindgut invagination, but whether the normal epithelial rearrangement of PMG invagination is required for morphogenesis of the connected tissues has been unclear. In <em>steppke</em> mutants, epithelial organization of the PMG primordium is strongly disrupted. Despite this disruption, germband extension and hindgut invagination are remarkably effective, and involve myosin network inductions known to promote their wild-type remodelling. Known tissue-autonomous signaling could explain the planar-polarized, junctional myosin networks of the germband, but pushing forces from PMG invagination have been implicated in inducing apical myosin networks of the hindgut primordium. To confirm that the wave of hindgut primordium myosin accumulations is due to mechanical effects, rather than diffusive signalling, we analyzed α-catenin RNAi embryos, in which all of the epithelial tissues initially form but then lose cell-cell adhesion, and observed strongly diminished hindgut primordium myosin accumulations. Thus, alternate mechanical changes in <em>steppke</em> mutants seem to circumvent the lack of normal PMG invagination to induce hindgut myosin networks and invagination. Overall, both germband extension and hindgut invagination are robust to experimental disruption of the PMG invagination, and, although the processes occur with some abnormalities in <em>steppke</em> mutants, there is remarkable redundancy in the multi-tissue system. Such redundancy could allow complex morphogenetic processes to change over evolutionary time.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"517 ","pages":"Pages 168-177"},"PeriodicalIF":2.5,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142399702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0