Developmental biology最新文献_第5页

Cranial neural crest shortage leads to extensive craniofacial anomalies in mice mutant for the NR2F1/2 nuclear receptors NR2F1/2核受体突变小鼠颅神经嵴缺失导致广泛颅面异常。

IF 2.1 3区生物学

Developmental biology Pub Date : 2026-02-01 Epub Date: 2025-11-21 DOI: 10.1016/j.ydbio.2025.11.011

David Paulding , Simon J.Y. Han , Jonathan Timmons , Michelle Caye , Alexa Riedel , Samantha A. Brugmann , Lindsey Barske

{"title":"Cranial neural crest shortage leads to extensive craniofacial anomalies in mice mutant for the NR2F1/2 nuclear receptors","authors":"David Paulding , Simon J.Y. Han , Jonathan Timmons , Michelle Caye , Alexa Riedel , Samantha A. Brugmann , Lindsey Barske","doi":"10.1016/j.ydbio.2025.11.011","DOIUrl":"10.1016/j.ydbio.2025.11.011","url":null,"abstract":"<div><div>Nuclear receptors are iteratively deployed during neural crest development, from pre-induction through differentiation stages. NR2F1 and NR2F2 in particular have been proposed as broad regulators of early neural crest gene expression in mammals, but the timing, extent, and redundancy of their developmental requirement has remained unclear, as <em>Nr2f1</em> and <em>Nr2f2</em> single mouse mutants present only minimal craniofacial phenotypes. Here we report the dynamic expression patterns of <em>Nr2f1</em> and <em>Nr2f2</em> in the mouse cranial neural crest from specification through post-migratory stages. Combined conditional knockout of both <em>Nr2f1</em> and <em>Nr2f2</em> in the neural crest with <em>Wnt1-</em>Cre or <em>Pax3</em><sup>Cre</sup> caused severe midfacial clefting, loss of the maxilla and palate, and hypoplasticity of all other facial skeletal elements except the distal mandible. These perinatal phenotypes were rooted in a major shortage of pharyngeal arch mesenchyme at mid-gestation. This in turn traced to a deficiency of migrating neural crest cells, first evident in the trailing part of the first arch migratory stream at embryonic day 8.75. RNAseq at a slightly earlier stage revealed downregulation of many migratory neural crest genes, including a possible direct target, the phospholipase <em>Plcg2</em>. These findings reveal a vital requirement for NR2F1/2 within the later-forming cranial neural crest.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"530 ","pages":"Pages 102-118"},"PeriodicalIF":2.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145586210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optogenetic activation of primary sensory neurons induces metamorphosis in Ciona 光遗传激活初级感觉神经元诱导小鼠变形。

IF 2.1 3区生物学

Developmental biology Pub Date : 2026-02-01 Epub Date: 2025-11-20 DOI: 10.1016/j.ydbio.2025.11.014

Nozomu M. Totsuka, Kohji Hotta

{"title":"Optogenetic activation of primary sensory neurons induces metamorphosis in Ciona","authors":"Nozomu M. Totsuka, Kohji Hotta","doi":"10.1016/j.ydbio.2025.11.014","DOIUrl":"10.1016/j.ydbio.2025.11.014","url":null,"abstract":"<div><div>Metamorphosis is a key event in development that is conserved in many marine organisms. <em>Ciona intestinalis</em> type A induces metamorphosis through the settlement of papillae onto the substrate. The papilla consists of collocytes (CCs), primary sensory neurons (PSNs), and axial columnar cells (ACCs), but it remains unclear whether PSNs alone can induce metamorphosis. Manipulating single neurons is crucial for elucidating the neural network system that drives metamorphosis. In this study, we developed an optogenetic system in which ChrimsonR, a red-shifted mutant of channelrhodopsin, was expressed exclusively in PSNs, enabling metamorphosis to be induced by light stimulation. A <em>Ciona</em>-optimized self-cleaving peptide, T2A, was used to co-express the Ca<sup>2+</sup> indicator GCaMP6s, allowing us to monitor neural activity during light stimulation. Activation of PSNs alone induced a series of metamorphic events, including epidermal backward movement, mesenchymal cell extravasation, and tail regression. Furthermore, we confirmed that metamorphosis proceeded to the juvenile stage. Metamorphosis was induced even with intermittent light stimulation, and the total stimulation time required for its initiation was approximately 6 min. The optogenetic system developed in this study may significantly contribute to elucidating the link between neuronal function and metamorphosis at the single-cell level.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"530 ","pages":"Pages 77-85"},"PeriodicalIF":2.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145581925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PDGFRα is required for postnatal cerebral perivascular fibroblast development “PDGFRα是出生后大脑血管周围成纤维细胞发育所必需的”。

IF 2.1 3区生物学

Developmental biology Pub Date : 2026-02-01 Epub Date: 2025-11-14 DOI: 10.1016/j.ydbio.2025.11.003

Hannah E. Jones , Kelsey A. Abrams , Sol Kim , Katherine A. Fantauzzo , Julie A. Siegenthaler

{"title":"PDGFRα is required for postnatal cerebral perivascular fibroblast development","authors":"Hannah E. Jones , Kelsey A. Abrams , Sol Kim , Katherine A. Fantauzzo , Julie A. Siegenthaler","doi":"10.1016/j.ydbio.2025.11.003","DOIUrl":"10.1016/j.ydbio.2025.11.003","url":null,"abstract":"<div><div>Perivascular fibroblasts (PVFs) are a cell type associated with large diameter blood vessels in the brain and spinal cord parenchyma and leptomeninges. PVFs have previously defined roles in injury and neuroinflammatory diseases and predicted roles in supporting neurovascular function. The temporal dynamics of PVF development in the pre- and postnatal cerebral cortex have recently been described, however the molecular mechanisms that underly PVF development have not been identified. PVFs express both platelet-derived growth factor receptors (PDGFRs), PDGFRα and PDGFRβ. Here we investigate the role of PDGF signaling in PVF development. We use immunohistochemistry and RNA transcript detection methods to show developmental expression of PDGFRs by PVFs and examine distribution of PDGF ligand expression in the brain. We show that postnatal deletion of PDGFRα in fibroblasts using the <em>Col1a2-CreERT</em> mouse line impairs PVF coverage of cerebral vessels at postnatal day 10. Perivascular macrophages, a cell type previously shown to co-develop with PVFs, have impaired cerebral vessel coverage in conditional mutants that is similar to PVF coverage defects. This work establishes a requirement for PDGFRα signaling in PVF development and may shed light upon the potential pathways that are over-activated in PVFs in injury and disease contexts.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"530 ","pages":"Pages 1-11"},"PeriodicalIF":2.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145534058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polar bodies serve as a landmark for anteroposterior axis formation in spiders 极体是蜘蛛前后轴形成的一个标志。

IF 2.1 3区生物学

Developmental biology Pub Date : 2026-02-01 Epub Date: 2025-12-15 DOI: 10.1016/j.ydbio.2025.12.006

Ruixun Wang, Matthias Pechmann

{"title":"Polar bodies serve as a landmark for anteroposterior axis formation in spiders","authors":"Ruixun Wang, Matthias Pechmann","doi":"10.1016/j.ydbio.2025.12.006","DOIUrl":"10.1016/j.ydbio.2025.12.006","url":null,"abstract":"<div><div>The early embryogenesis of many spiders involves the formation of a radially symmetric germ disc. While the cells of the rim of this germ disc develops into anterior structures, the center of the disc will form the posteriorly located segment addition zone of the embryo. Therefore, germ disc formation sets the anterior-posterior (AP) body axis of spider embryos. The early spider egg is a spherical structure with no apparent asymmetry. So far it is unclear if the placement of the germ disc is a predetermined or a stochastic process.</div><div>For this study, we have re-analysed early spider embryogenesis and found a link between the position of the polar bodies and the formation of the germ disc. Using cell tracking and time-lapse recordings of live embryos injected with the live cell nuclear stain SPY555-DNA, we show that the germ disc forms opposite to the polar bodies. Our results suggest that germ disc formation in the common house spider <em>Parasteatoda tepidariorum</em> is not a stochastic but a pre-determined process. By analyzing germ disc formation in a basally branching cobweb spider and a curtain-web spider, we provide evidence that the initial process of germ disc placement might be conserved between araneomorph and mygalomorph spider species.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"530 ","pages":"Pages 265-273"},"PeriodicalIF":2.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145773766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ureteric stromal progenitors give rise to kidney inner cortical pericytes via an arterial mural cell intermediate 输尿管间质祖细胞经动脉壁细胞中间体形成肾内皮质周细胞。

IF 2.1 3区生物学

Developmental biology Pub Date : 2026-02-01 Epub Date: 2025-11-13 DOI: 10.1016/j.ydbio.2025.11.005

Peter M. Luo , Neha H. Ahuja , Thomas J. Carroll , Ondine Cleaver

{"title":"Ureteric stromal progenitors give rise to kidney inner cortical pericytes via an arterial mural cell intermediate","authors":"Peter M. Luo , Neha H. Ahuja , Thomas J. Carroll , Ondine Cleaver","doi":"10.1016/j.ydbio.2025.11.005","DOIUrl":"10.1016/j.ydbio.2025.11.005","url":null,"abstract":"<div><div>During kidney formation, segmented epithelial tubules and blood vessels develop within a heterogeneous and progressively patterned stroma. By E18.5, the murine renal stroma exhibits several transcriptionally and spatially distinct populations, including specialized stromal cells associated with the vasculature, termed mural cells. However, the precise contributions of stromal progenitor lineages to this stromal heterogeneity, as well as the dynamics of renal mural cell investment, remain unclear. Previous studies have described stromal progenitors in the developing cortex that transiently express the transcription factor <em>Foxd1,</em> as well as stromal progenitors in the ureter that express <em>Tbx18</em>, and have shown that both are capable of giving rise to renal stromal cells, including vascular mural cells. Here, we use pulse induction of Tbx19CreERT2 at different timepoints to elucidate the contribution of the <em>Tbx18</em> population to stromal patterning. We show that the <em>Tbx18-</em>lineage, when induced at E12.5, gives rise to arterial mural cells, without ever progressing through a <em>Foxd1+</em> cortical stromal progenitor state. These arterial mural cells are only transiently present along arteries during development, ultimately contributing instead to peritubular capillaries. When traced post-natally, the <em>Tbx18-</em>lineage gives rise to pericytes, which are enriched in S3-segment-associated, <em>Cxcl14</em>-enriched stroma in the inner cortex. We show that these pericytes arise directly from arterial mural cells seen earlier during development. These data help clarify a small portion of the complicated lineage relationships of renal stromal progenitors and their contribution to the kidney vascular-associated mural cells.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"530 ","pages":"Pages 86-101"},"PeriodicalIF":2.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145530487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epicardial Tcf21 facilitates cardiomyocyte dedifferentiation and heart regeneration in zebrafish 心外膜Tcf21促进斑马鱼心肌细胞去分化和心脏再生。

IF 2.1 3区生物学

Developmental biology Pub Date : 2026-02-01 Epub Date: 2025-12-06 DOI: 10.1016/j.ydbio.2025.12.004

Miglė Kalvaitytė-Repečkė , Sofija Gabrilavičiūtė , Kotryna Kvederavičiūtė , Leonard Burg , Edita Bakūnaitė , Kenneth D. Poss , Darius Balciunas

{"title":"Epicardial Tcf21 facilitates cardiomyocyte dedifferentiation and heart regeneration in zebrafish","authors":"Miglė Kalvaitytė-Repečkė , Sofija Gabrilavičiūtė , Kotryna Kvederavičiūtė , Leonard Burg , Edita Bakūnaitė , Kenneth D. Poss , Darius Balciunas","doi":"10.1016/j.ydbio.2025.12.004","DOIUrl":"10.1016/j.ydbio.2025.12.004","url":null,"abstract":"<div><div>Unlike mammals, zebrafish (<em>Danio rerio</em>) are able to regenerate their hearts after injury, making them an excellent model organism for studying the molecular mechanisms underlying heart regeneration. Epicardium, the outermost layer of the heart, is an essential player in this process. Injury-induced epicardium activation, characterized by the expression of embryonic epicardial marker genes including <em>tcf21,</em> supports cardiac regeneration by providing various cell types and releasing paracrine signals that promote the restoration of damaged tissue. However, the molecular mechanisms involved in this process are insufficiently understood. In this study, we describe a conditional <em>tcf21</em><sup><em>flox</em></sup> allele and use it to investigate the role of Tcf21 in heart regeneration. By employing 4-hydroxytamoxifen inducible CreER<sup>T2</sup> recombinase, we eliminated <em>tcf21</em> expression in adult fish. Our findings indicate that loss of this transcription factor reduces the presence of dedifferentiated cardiomyocytes in the injury area and impairs heart regeneration. This work provides new insights into the molecular basis of the epicardial response to heart injury and its role in guiding heart regeneration.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"530 ","pages":"199"},"PeriodicalIF":2.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145707498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Direct activation of folate receptor 4 by thyroid hormone suggests its role in the development of adult intestinal epithelium during Xenopus laevis metamorphosis 叶酸受体4被甲状腺激素直接激活，提示其在非洲爪蟾变态成虫肠上皮发育过程中起重要作用。

IF 2.1 3区生物学

Developmental biology Pub Date : 2026-02-01 Epub Date: 2025-11-12 DOI: 10.1016/j.ydbio.2025.11.006

Kenta Fujimoto , Yuki Shibata , Morihiro Okada , Yun-Bo Shi , Takashi Hasebe

{"title":"Direct activation of folate receptor 4 by thyroid hormone suggests its role in the development of adult intestinal epithelium during Xenopus laevis metamorphosis","authors":"Kenta Fujimoto , Yuki Shibata , Morihiro Okada , Yun-Bo Shi , Takashi Hasebe","doi":"10.1016/j.ydbio.2025.11.006","DOIUrl":"10.1016/j.ydbio.2025.11.006","url":null,"abstract":"<div><div>Amphibian metamorphosis is tightly regulated by thyroid hormone (TH). During this process, most larval epithelial cells in the <em>Xenopus laevis</em> intestine undergo apoptosis, whereas a small population dedifferentiates into adult epithelial stem cells. They subsequently proliferate and differentiate to form a trough-crest epithelial architecture similar to the mammalian crypt-villus axis. We have previously identified a number of TH-responsive genes likely involved in this intestinal remodeling. Here, we focus on one such gene, <em>folate receptor 4</em> (<em>folr4</em>). We examined the spatiotemporal expression of <em>folr4.L</em> by using quantitative RT-PCR and <em>in situ</em> hybridization chain reaction (HCR) and found that <em>folr4.L</em> expression is highly upregulated during both natural and TH-induced metamorphosis. Interestingly, in the epithelium at the climax of metamorphosis, <em>folr4.L</em> is specifically expressed in the proliferating and/or differentiating adult epithelial cells located adjacent to proliferating adult stem cells, which express intestinal stem cell marker <em>leucine-rich repeat-containing G protein-coupled 5</em> (<em>lgr5</em>). Moreover, we identified a TH response element (TRE) in the <em>folr4.L</em> promoter that binds to the heterodimer of TH receptor (TR) and 9-cis retinoic acid receptor (RXR) <em>in vitro</em> and mediates T3-dependent transcriptional activation <em>in vivo.</em> Phylogenetic analysis suggested that <em>X. laevis</em> Folr4.L may be more closely related to riboflavin binding protein (Rfbp) than mammalian FOLR4. These findings suggest that TH-induced Folr4.L might be involved in the development of adult intestinal epithelium.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"530 ","pages":"Pages 12-20"},"PeriodicalIF":2.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145523093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Challenges and opportunities for understanding the genetic causes of congenital anomalies 了解先天性异常遗传原因的挑战与机遇。

IF 2.1 3区生物学

Developmental biology Pub Date : 2026-02-01 Epub Date: 2025-12-01 DOI: 10.1016/j.ydbio.2025.12.001

Janina Kueper , Ivan Moskowitz , Rolf Stottmann , Irene Zohn , Mustafa K. Khokha

引用次数: 0

Cse1l is critical for cell survival, craniofacial and cardiac development csel1对细胞存活、颅面和心脏发育至关重要

IF 2.1 3区生物学

Developmental biology Pub Date : 2026-02-01 Epub Date: 2025-12-09 DOI: 10.1016/j.ydbio.2025.12.007

Paul P.R. Iyyanar , Rolf W. Stottmann

{"title":"Cse1l is critical for cell survival, craniofacial and cardiac development","authors":"Paul P.R. Iyyanar , Rolf W. Stottmann","doi":"10.1016/j.ydbio.2025.12.007","DOIUrl":"10.1016/j.ydbio.2025.12.007","url":null,"abstract":"<div><div>Human congenital anomalies account for twice the mortality of childhood cancer. Despite advancements in genome sequencing and transgenic mouse models that have aided in understanding their pathogenesis, significant gaps remain. Through a forward genetics approach, we previously discovered the hypo-morphic <em>anteater</em> allele of <em>Cse1l</em> which displayed variable craniofacial phenotypes. To circumvent the variability seen in this model, we generated a conditional allele of <em>Cse1l</em> and genetically ablated it in the dorsal midline giving rise to portions of the nervous system and the cranial neural crest cells using the <em>Wnt1-Cre</em>2 driver. Our analysis revealed that <em>Wnt1-Cre2; Cse1l</em><sup><em>CRISPR/flox</em></sup> embryos exhibited severe malformations in the forebrain, midbrain, and hindbrain, accompanied by a dramatic hypoplasia of the frontonasal, maxillary, and mandibular processes, and the second pharyngeal arch. <em>Wnt1-Cre2; Cse1l</em><sup><em>CRISPR/flox</em></sup> embryos were embryonic lethal by E11.5 likely due to proliferative defects in the ventricular myocardium. <em>Wnt1-Cre2; Cse1l</em><sup><em>CRISPR/flox</em></sup> embryos exhibited consistently increased apoptosis at E9.5 in the affected tissues along with an increase in p53 expression. These data together show a previously unknown critical function of CSE1L in neural crest cell survival during development.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"530 ","pages":"Pages 188-198"},"PeriodicalIF":2.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Environmental yin-yang: how external cues are processed at the level of cells and at the level of an organism 环境阴阳：如何在细胞和有机体的水平上处理外部线索。

IF 2.1 3区生物学

Developmental biology Pub Date : 2026-02-01 Epub Date: 2025-11-19 DOI: 10.1016/j.ydbio.2025.11.013

Zainab Afzal, Deepak Kumar

引用次数: 0