Developmental biology最新文献

{"title":"Mutant analysis in a small weed reveals the blueprint of floral patterning","authors":"Zhongchi Liu","doi":"10.1016/j.ydbio.2025.02.014","DOIUrl":"10.1016/j.ydbio.2025.02.014","url":null,"abstract":"<div><div>Under the theme “Research that Transformed Developmental Biology”, this article discusses the seminal work by Bowman, Smyth, and Meyerowitz published in Development in 1991. The work laid down an elegant blueprint of flower patterning, namely the ABC model of flower development, which remains a cornerstone of plant development. In addition to summarizing key aspects of the model, the article emphasizes several noteworthy aspects, including the basic technique (mutant analysis) employed and the influence by animal development. Additionally, the article discusses significant follow-up research that confirmed key aspects of the model. Lastly, the article examines the impact of the ABC model on the field of plant EvoDevo. Collectively, the journey of how basic genetic analysis of mutants resulted in groundbreaking advancements in plant development will inspire future scientists and drive transformative research forward.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"521 ","pages":"Pages 138-141"},"PeriodicalIF":2.5,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143478585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The transmembrane glycoprotein Gpnmb is required for the immune and fibrotic responses during zebrafish heart regeneration","authors":"Savita Gupta ,&nbsp;Gursimran Kaur Bajwa ,&nbsp;Hadil El-Sammak ,&nbsp;Kenny Mattonet ,&nbsp;Stefan Günther ,&nbsp;Mario Looso ,&nbsp;Didier Y.R. Stainier ,&nbsp;Rubén Marín-Juez","doi":"10.1016/j.ydbio.2025.02.011","DOIUrl":"10.1016/j.ydbio.2025.02.011","url":null,"abstract":"<div><div>Myocardial infarction occurs when the coronary supply of oxygen and nutrients to part of the heart is interrupted. In contrast to adult mammals, adult zebrafish have a remarkable ability to regenerate their heart after cardiac injury. Several processes are involved in this regenerative response including inflammation, coronary endothelial cell proliferation and revascularization, endocardial expansion, cardiomyocyte repopulation, and transient scar formation. To identify additional regulators of zebrafish cardiac regeneration, we profiled the transcriptome of regenerating coronary endothelial cells at 7 days post cryoinjury (dpci) and observed the significant upregulation of dozens of genes including <em>gpnmb</em>. Gpnmb (glycoprotein non-metastatic melanoma protein B) is a transmembrane glycoprotein implicated in inflammation resolution and tissue regeneration. Transcriptomic profiling data of cryoinjured zebrafish hearts reveal that <em>gpnmb</em> is mostly expressed by macrophages. To investigate <em>gpnmb</em> function during zebrafish cardiac regeneration, we generated a full locus deletion allele. We find that after cardiac cryoinjury, animals lacking <em>gpnmb</em> exhibit neutrophil retention and decreased macrophage recruitment as well as reduced myofibroblast numbers. Moreover, loss of <em>gpnmb</em> impairs coronary endothelial cell regeneration and cardiomyocyte dedifferentiation. Transcriptomic analyses of cryoinjured <em>gpnmb</em>^{<em>−/−</em>} hearts identified enhanced collagen gene expression and the activation of extracellular matrix (ECM) related pathways. Furthermore, <em>gpnmb</em>^{<em>−/−</em>} hearts exhibit larger fibrotic scars revealing additional defects in cardiac regeneration. Altogether, these data indicate that <em>gpnmb</em>, which is mostly expressed by macrophages, modulates inflammation and ECM deposition after cardiac cryoinjury in zebrafish and further highlight the importance of these immune cells during regeneration.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"521 ","pages":"Pages 153-162"},"PeriodicalIF":2.5,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143472270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From Mendel’s peas to genetic regulation of embryogenesis","authors":"Abraham Fainsod ,&nbsp;Martin Blum","doi":"10.1016/j.ydbio.2025.02.012","DOIUrl":"10.1016/j.ydbio.2025.02.012","url":null,"abstract":"<div><div>As scientists, we all stand on the shoulders of giants. This seemingly trivial metaphor reminds us to acknowledge those who laid the groundwork for our careers, to pass on this historical knowledge to future generations, and to cultivate a sense of modesty. In this regard, two prominent biologists from the mid-19th century, Gregor Mendel and Charles Darwin, stand out. Mendel's groundbreaking work laid the foundation for our understanding of genetics, while Darwin's theory illuminated the ever-changing world of plants and animals through the process of evolution. The principles they highlighted proved fundamental for our understanding of embryogenesis. The developmental process from a fertilized egg to an adult organism is paramount for the healthy creation of progeny, whether plants or animals. As with any biological process, embryogenesis is controlled by genes and is greatly impacted by changes in the environment. Pioneers in studying the genetic contribution to normal embryonic development included, among many others, Salome Guecksohn-Waelsch, Ed Lewis, Walter Gehring, Christiane Nüsslein-Volhard, and Eric Wieschaus. They made significant contributions to our understanding of this process, and their work continues to offer valuable insights, which is the subject of this article.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"521 ","pages":"Pages 122-128"},"PeriodicalIF":2.5,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143457173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outside Back Cover - Graphical abstract TOC/TOC in double column/Cover image legend if applicable, Bar code, Abstracting and Indexing information","authors":"","doi":"10.1016/S0012-1606(25)00044-2","DOIUrl":"10.1016/S0012-1606(25)00044-2","url":null,"abstract":"","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"520 ","pages":"Page OBC"},"PeriodicalIF":2.5,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143420726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Embryonic feather bud development – A keystone model for vertebrate organogenesis","authors":"Jon Riddell,&nbsp;Denis Headon","doi":"10.1016/j.ydbio.2025.02.010","DOIUrl":"10.1016/j.ydbio.2025.02.010","url":null,"abstract":"<div><div>The development of feathers in the embryonic skin has been used as a model for biological self-organisation for many decades. The availability, size and ease of manipulation of the skin has enabled it to serve as a model revealing concepts of epithelial-mesenchymal interaction, origins of periodic patterns in the anatomy, and the effects of growth factors and structural and mechanical properties on tissue development. These efforts provide a rich history of observation, informing continued development of new concepts in this system. Here we review the process of early feather bud development, the understanding gained from decades of experimentation, and current debate and future directions for progress.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"521 ","pages":"Pages 142-148"},"PeriodicalIF":2.5,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143425144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CMTM4 is an adhesion modulator that regulates skeletal patterning and primary mesenchyme cell migration in sea urchin embryos","authors":"Abigail E. Descoteaux ,&nbsp;Marko Radulovic ,&nbsp;Dona Alburi ,&nbsp;Cynthia A. Bradham","doi":"10.1016/j.ydbio.2025.02.009","DOIUrl":"10.1016/j.ydbio.2025.02.009","url":null,"abstract":"<div><div>MARVEL proteins, including those of the CMTM gene family, are multi-pass transmembrane proteins that play important roles in vesicular trafficking and cell migration; however, little is understood about their role in development, and their role in skeletal patterning is unexplored. CMTM4 is the only CMTM family member found in the developmental transcriptome of the sea urchin <em>Lytechinus variegatus</em>. Here, we validate that LvCMTM4 is a transmembrane protein and show that perturbation of CMTM4 expression via zygotic morpholino or mRNA injection perturbs skeletal patterning, resulting in loss of secondary skeletal elements and rotational defects. We also demonstrate that normal levels of CMTM4 are required for normal PMC migration and filopodial organization, and that these effects are not due to gross mis-specification of the ectoderm. Finally, we show that CMTM4 is sufficient to mediate mesenchymal cell-cell adhesion. Taken together, these data suggest that CMTM4 controls PMC migration and biomineralization via adhesive regulation during sea urchin skeletogenesis. This is the first discovery of a functionally required adhesive gene in this skeletal patterning system.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"521 ","pages":"Pages 85-95"},"PeriodicalIF":2.5,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143413690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The chicken embryo brings new insights into the evolutionary role of WFDC1 during amniote development","authors":"Thaís Metzker-Pinto ,&nbsp;Yen T.H. Tran ,&nbsp;Igor Buzzatto-Leite ,&nbsp;Lloyd Lok ,&nbsp;Jórdan F. Sampar ,&nbsp;Hernandes F. Carvalho ,&nbsp;Gonzalo del Monte-Nieto ,&nbsp;Lúcia E. Alvares","doi":"10.1016/j.ydbio.2025.02.008","DOIUrl":"10.1016/j.ydbio.2025.02.008","url":null,"abstract":"<div><div><em>WFDC1</em> encodes an extracellular matrix protein involved in cell proliferation, migration, and epithelial-mesenchymal transition in disease conditions. Despite this, <em>Wfdc1-null</em> mice display no discernible malformations while cattle bearing a <em>WFDC1</em> mutation present multiple ocular defects, leaving the role of <em>WFDC1</em> during embryonic development unclear. To address this, we used the chicken embryo as a model to investigate <em>WFDC1</em> developmental roles in amniotes. We performed a comparative expression analysis during chicken and mouse development, which revealed expression in ectodermal and mesodermal derivatives, with both conserved and species-specific domains. Conserved expression was observed in the eye, otic vesicle, central and peripheral nervous systems, and neural crest cells. Chicken-specific expression was identified in mesodermal structures, including the notochord, limbs and heart. However, even in the conserved sites like the eyes, <em>WFDC1</em> localizes to different retinal layers, indicating potential divergence roles in retinal development and function across species. In contrast, <em>WFDC1</em> expression in the limb buds is specific to chicken, encompassing the distal mesenchyme, interdigital membranes, and blastemas. Functional enrichment analysis links <em>WFDC1</em> to limb patterning, morphogenesis, and Wnt signaling. The species-specific differences likely stem from evolutionary changes in gene regulation, supported by differences in proximal cis-regulatory elements of the <em>WFDC1</em> loci between chicken and mouse. The complexity of <em>WFDC1</em> expression in the chicken embryo, along with <em>WFDC1</em> regulatory conservation within birds, indicates that this gene may play specific roles in avian development, possibly contributing to features specific to this lineage. Future studies using the chicken model will be valuable in further uncovering the specific roles of <em>WFDC1</em>.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"521 ","pages":"Pages 96-107"},"PeriodicalIF":2.5,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143412828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PRDM14 is essential for vertebrate gastrulation and safeguards avian germ cell identity","authors":"Dadakhalandar Doddamani ,&nbsp;Daniel F. Carlson ,&nbsp;Lynn McTeir ,&nbsp;Lorna Taylor ,&nbsp;Sunil Nandi ,&nbsp;Megan G. Davey ,&nbsp;Mike J. McGrew ,&nbsp;James D. Glover","doi":"10.1016/j.ydbio.2025.02.005","DOIUrl":"10.1016/j.ydbio.2025.02.005","url":null,"abstract":"<div><div>The zinc finger transcription factor PRDM14, part of the PR domain containing protein family, is critical for mammalian primordial germ cell (PGC) specification, epigenetic reprogramming and maintaining naïve pluripotency in stem cells. However, PRDM14's role in other species is not well understood. In chicken, <em>PRDM14</em> is broadly expressed in the early embryo, before becoming restricted to the forming neural plate, migratory PGCs, and later, in the adult testes. To investigate the role of PRDM14 we generated two independent targeted chicken lines and bred homozygous knockout embryos. Strikingly, we found that gastrulation was disrupted in <em>PRDM14</em>^{<em>−/−</em>} embryos, which lacked a definitive primitive streak. Transcriptomic and <em>in situ</em> hybridisation analyses revealed a broad loss of anterior primitive streak marker genes, coupled with downregulation of the multifunctional antagonists <em>CHRD</em> and <em>CER1</em><em>,</em> and expansion of the <em>NODAL</em> expression domain. Further analysis of <em>PRDM14</em>^{<em>−/−</em>} embryos revealed PGCs were still specified but significantly reduced in number, and <em>PRDM14</em>^{<em>−/−</em>} PGCs could not be propagated <em>in vitro</em>. Knockdown studies <em>in vitro</em> confirmed that PRDM14 is essential for PGC survival and antagonises FGF-induced somatic differentiation, similar to PRDM14's role in mammalian stem cells. Taken together, our results show that in chicken, PRDM14 plays a multifunctional and essential role during embryonic development.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"521 ","pages":"Pages 129-137"},"PeriodicalIF":2.5,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143405319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The three-dimensional structure of medullary bone: Novel criteria for the identification of avian sex-specific bone tissue","authors":"Abi H. Crane ,&nbsp;Claudia J. Baldry ,&nbsp;Kathryn E. Rankin ,&nbsp;Claire E. Clarkin ,&nbsp;Katherine A. Williams ,&nbsp;Neil J. Gostling","doi":"10.1016/j.ydbio.2025.02.007","DOIUrl":"10.1016/j.ydbio.2025.02.007","url":null,"abstract":"<div><div>Medullary bone is a fast-growing, ephemeral bone tissue found inside the bone cavities of female birds. Identifying this tissue in the bones of fossil avian and non-avian dinosaurs has the potential to determine which specimens represent reproductively mature females. However, difficulties in distinguishing medullary bone from superficially similar bone pathologies has led to uncertainty as to whether some specimens previously thought to contain medullary bone instead represent sick or injured individuals. The most frequently mentioned of these pathologies is avian osteopetrosis, a virally-induced condition in birds causing bony lesions which can resemble medullary bone. Lists of criteria, primarily using two-dimensional osteohistology, have yet to form a comprehensive framework through which all medullary bone can be positively identified, and all pathology excluded. Here, we use high-resolution computed tomography (μCT) to characterise the three-dimensional structure of medullary bone in modern birds for the first time and make comparisons to the endosteal lesions of avian osteopetrosis. We identify both qualitative and quantitative features which we suggest to be characteristic of medullary bone, including connectivity density and osteocyte lacunar orientation, and highlight conspicuously variable features which require further investigation. We find several three-dimensional which can be used to differentiate between medullary bone and avian osteopetrosis, including structural anisotropy and trabecular thickness. These three-dimensional characters can be added to the growing framework of criteria to identify medullary bone in the fossil record and thus help determine the sex of dinosaurs.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"521 ","pages":"Pages 108-121"},"PeriodicalIF":2.5,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143405623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomic analysis of chick embryonic heart in experimental hypoxia","authors":"David Sedmera ,&nbsp;Eliska Drobna Krejci ,&nbsp;Ondrej Nanka ,&nbsp;Adam Eckhardt","doi":"10.1016/j.ydbio.2025.02.006","DOIUrl":"10.1016/j.ydbio.2025.02.006","url":null,"abstract":"<div><div>Investigating prenatal hypoxia is difficult in mammals, as there are confounding factors stemming from maternal adaptations and compensatory mechanisms. We have thus established an avian model of hypoxic incubation (starting after 2 days of normoxia, 15% O₂, normobaric, until the time of sampling at embryonic day 8) to study embryonic reactions to low oxygen concentration. Our previous studies have shown increased vascularization, oedema, and ventricular wall thinning preceding the lethality at mid-gestation. Analysis of the cardiac proteome after 6 days of hypoxic incubation showed strong upregulation of enzymes involved in anaerobic glycolysis as well as an increase in apoptosis-related proteins, cell adhesion proteins, and secretory activity.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"521 ","pages":"Pages 28-36"},"PeriodicalIF":2.5,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143394369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}