Developmental biology最新文献_第5页

Surrounding tissue morphogenesis with disrupted posterior midgut invagination during Drosophila gastrulation 果蝇胃形成过程中后部中肠内陷的周围组织形态发生紊乱

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-10-09 DOI: 10.1016/j.ydbio.2024.10.001

Sandra Sabbagh, Tony J.C. Harris

{"title":"Surrounding tissue morphogenesis with disrupted posterior midgut invagination during Drosophila gastrulation","authors":"Sandra Sabbagh, Tony J.C. Harris","doi":"10.1016/j.ydbio.2024.10.001","DOIUrl":"10.1016/j.ydbio.2024.10.001","url":null,"abstract":"<div><div>Gastrulation involves multiple, physically-coupled tissue rearrangements. During Drosophila gastrulation, posterior midgut (PMG) invagination promotes both germband extension and hindgut invagination, but whether the normal epithelial rearrangement of PMG invagination is required for morphogenesis of the connected tissues has been unclear. In <em>steppke</em> mutants, epithelial organization of the PMG primordium is strongly disrupted. Despite this disruption, germband extension and hindgut invagination are remarkably effective, and involve myosin network inductions known to promote their wild-type remodelling. Known tissue-autonomous signaling could explain the planar-polarized, junctional myosin networks of the germband, but pushing forces from PMG invagination have been implicated in inducing apical myosin networks of the hindgut primordium. To confirm that the wave of hindgut primordium myosin accumulations is due to mechanical effects, rather than diffusive signalling, we analyzed α-catenin RNAi embryos, in which all of the epithelial tissues initially form but then lose cell-cell adhesion, and observed strongly diminished hindgut primordium myosin accumulations. Thus, alternate mechanical changes in <em>steppke</em> mutants seem to circumvent the lack of normal PMG invagination to induce hindgut myosin networks and invagination. Overall, both germband extension and hindgut invagination are robust to experimental disruption of the PMG invagination, and, although the processes occur with some abnormalities in <em>steppke</em> mutants, there is remarkable redundancy in the multi-tissue system. Such redundancy could allow complex morphogenetic processes to change over evolutionary time.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"517 ","pages":"Pages 168-177"},"PeriodicalIF":2.5,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142399702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Drosophila anterior midgut internalization via collective epithelial-mesenchymal transition at the embryo surface and enclosure by surrounding tissues 果蝇前中肠通过胚胎表面的集体上皮-间质转化和周围组织的包围而内化。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-10-09 DOI: 10.1016/j.ydbio.2024.10.002

Sandra Sabbagh, Hui Zhang, Tony J.C. Harris

{"title":"Drosophila anterior midgut internalization via collective epithelial-mesenchymal transition at the embryo surface and enclosure by surrounding tissues","authors":"Sandra Sabbagh, Hui Zhang, Tony J.C. Harris","doi":"10.1016/j.ydbio.2024.10.002","DOIUrl":"10.1016/j.ydbio.2024.10.002","url":null,"abstract":"<div><div>Internal organ development requires cell internalization, which can occur individually or collectively. The best characterized mode of collective internalization is epithelial invagination. Alternate modes involving collective mesenchymal behaviours at the embryo surface have been documented, but their prevalence is unclear. The Drosophila embryo has been a major model for the study of epithelial invaginations. However, internalization of the Drosophila anterior midgut primordium is incompletely understood. Here, we report that an epithelial-mesenchymal transition (EMT) occurs across the internalizing primordium when it is still at the embryo surface. At the earliest internalization stage, the primordium displays less junctional DE-cadherin than surrounding tissues but still exhibits coordinated epithelial structure as it invaginates with the ventral furrow. This initial invagination is transient, and its loss correlates with the activation of an associated mitotic domain. Activation of a subsequent mitotic domain across the broader primordium results in cell divisions with mixed orientations that deposit some cells within the embryo. However, cell division is non-essential for primordium internalization. Post-mitotically, the surface primordium displays hallmarks of EMT: loss of adherens junctions, loss of epithelial cell polarity, and gain of cell protrusions. Primordium cells extend over each other as they internalize asynchronously as individuals or small groups, and the primordium becomes enclosed by the reorganizations of surrounding epithelial tissues. We propose that collective EMT at the embryo surface promotes anterior midgut internalization through both inwardly-directed divisions and movements of its cells, and that the latter process is facilitated by surrounding tissue remodeling.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"517 ","pages":"Pages 191-202"},"PeriodicalIF":2.5,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142406227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Guiding Students to Think Critically about Reproductive Development and Public Conversations on Sex and Gender. 引导学生批判性地思考生殖发展和有关性与性别的公共对话。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-10-05 DOI: 10.1016/j.ydbio.2024.09.012

Megan Morgan Hoffman

引用次数: 0

Modelling and calibration of pair-rule protein patterns in Drosophila embryo: From Even-skipped and Fushi-tarazu to Wingless expression networks 果蝇胚胎中成对规则蛋白质模式的建模和校准：从偶数跳和傅氏太郎到无翼表达网络。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-10-04 DOI: 10.1016/j.ydbio.2024.09.015

Catarina Dias, Rui Dilão

引用次数: 0

Soma-to-germline BMP signal is essential for Drosophila spermiogenesis 体节到生殖系的BMP信号对果蝇的精子形成至关重要

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-10-02 DOI: 10.1016/j.ydbio.2024.09.016

Emma Kristine Beard, Rachael P. Norris, Miki Furusho, Mark Terasaki, Mayu Inaba

{"title":"Soma-to-germline BMP signal is essential for Drosophila spermiogenesis","authors":"Emma Kristine Beard, Rachael P. Norris, Miki Furusho, Mark Terasaki, Mayu Inaba","doi":"10.1016/j.ydbio.2024.09.016","DOIUrl":"10.1016/j.ydbio.2024.09.016","url":null,"abstract":"<div><div>In the <em>Drosophila</em> testis, developing germ cells are encapsulated by somatic support cells throughout development. Soma-germline interactions are essential for successful spermiogenesis. However, it is still not fully understood what signaling events take place between the soma and the germline. In this study, we found that a Bone Morphogenetic Protein (BMP) ligand, Glass bottom boat (Gbb), secreted from somatic cyst cells (CCs), signals to differentiating germ cells to maintain proper spermiogenesis. Knockdown of Gbb in CCs or the type I BMP receptor Saxophone (Sax) in germ cells leads to a defect in sperm head bundling and decreased fertility. Our Transmission Electron Microscopy (TEM) analyses revealed that the mutant germ cells have aberrant morphology of mitochondria throughout the stages of spermiogenesis and exhibit a defect in nebenkern formation. Elongating spermatids show uncoupled nuclei and elongating mitochondrial derivatives, suggesting that improper mitochondrial development may cause sperm bundling defects. Taken together, we propose a new role of soma-derived BMP signaling, which is essential for spermiogenesis.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"517 ","pages":"Pages 140-147"},"PeriodicalIF":2.5,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142371237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Left/right asymmetrically expressed ephrin and Flamingo proteins regulate lateralized axon growth in C. elegans 左右不对称表达的ephrin和Flamingo蛋白调控秀丽隐杆线虫侧向轴突生长

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-09-26 DOI: 10.1016/j.ydbio.2024.09.014

Khulganaa Buyannemekh , Paul Villoutreix , Vincent Bertrand

{"title":"Left/right asymmetrically expressed ephrin and Flamingo proteins regulate lateralized axon growth in C. elegans","authors":"Khulganaa Buyannemekh , Paul Villoutreix , Vincent Bertrand","doi":"10.1016/j.ydbio.2024.09.014","DOIUrl":"10.1016/j.ydbio.2024.09.014","url":null,"abstract":"<div><div>While the nervous system of bilaterian animals is mainly left-right (L-R) symmetric at the anatomical level, some molecular and functional L-R asymmetries exist. However, the extent of these molecular asymmetries and their functional consequences remain poorly characterized. <em>C. elegans</em> allows to study L-R asymmetries in the nervous system with single-neuron resolution. We have previously shown that a neural bHLH transcription factor, HLH-16/Olig, is L-R asymmetrically expressed in the AIY neuron lineage and regulates AIY axon projections in a L-R asymmetric manner. Here, by combining a candidate approach and single-cell RNA sequencing data analysis, we identify the ephrin protein EFN-2 and the Flamingo protein FMI-1 as downstream targets of HLH-16 that are L-R asymmetrically expressed in the AIY lineage. We show that EFN-2 and FMI-1 collaborate in the L-R asymmetric regulation of axonal growth. EFN-2 may act via a non-canonical receptor of the L1CAM family, SAX-7. Our study reveals novel molecular L-R asymmetries in the <em>C. elegans</em> nervous system and their functional consequences.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"517 ","pages":"Pages 117-125"},"PeriodicalIF":2.5,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142343359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Zebrafish Foxl2l functions in proliferating germ cells for female meiotic entry 斑马鱼 Foxl2l 在增殖生殖细胞中发挥着雌性减数分裂入口的功能。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-09-26 DOI: 10.1016/j.ydbio.2024.09.013

Ching-Hsin Yang , Yan-wei Wang , Chen-wei Hsu , Bon-chu Chung

{"title":"Zebrafish Foxl2l functions in proliferating germ cells for female meiotic entry","authors":"Ching-Hsin Yang , Yan-wei Wang , Chen-wei Hsu , Bon-chu Chung","doi":"10.1016/j.ydbio.2024.09.013","DOIUrl":"10.1016/j.ydbio.2024.09.013","url":null,"abstract":"<div><div>Zebrafish sex differentiation is a complicated process and the detailed mechanism has not been fully understood. Here we characterized a transcription factor, Foxl2l, which participates in female oogenesis. We show that it is expressed specifically in proliferating germ cells in juvenile gonads and mature ovaries. We have used CRISPR-Cas9 to generate zebrafish deficient in <em>foxl2l</em> expression. Zebrafish with <em>foxl2l</em><sup><em>−/−</em></sup> are all males, and this female-to-male sex reversal cannot be reversed by <em>tp53</em> mutation, indicating this sex reversal is unrelated to cell death. We have generated transgenic fish expressing GFP under the control of <em>foxl2l</em> promoter to track the development of <em>foxl2l</em> + -germ cells; these cells failed to enter meiosis and accumulated as cystic cells in the <em>foxl2l</em><sup><em>−/−</em></sup> mutant. Our RNA-seq analysis also showed the reduced expression of genes in meiosis and oogenesis among other affected pathways. All together, we show that zebrafish Foxl2l is a nuclear factor controlling the expression of meiotic and oogenic genes, and its deficiency leads to defective meiotic entry and the accumulation of premeiotic germ cells.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"517 ","pages":"Pages 91-99"},"PeriodicalIF":2.5,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142343362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pax2a, Pax5 and Cdh1-β-catenin, but not Wnt, protect sensory hair cells from destabilizing effects of fgf signaling on cell adhesion Pax2a、Pax5和Cdh1-β-catenin（而非Wnt）能保护感觉毛细胞免受Fgf信号对细胞粘附的不稳定影响。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-09-25 DOI: 10.1016/j.ydbio.2024.09.009

Whitney Roberson, Jorden N. Holland, Bruce B. Riley

{"title":"Pax2a, Pax5 and Cdh1-β-catenin, but not Wnt, protect sensory hair cells from destabilizing effects of fgf signaling on cell adhesion","authors":"Whitney Roberson, Jorden N. Holland, Bruce B. Riley","doi":"10.1016/j.ydbio.2024.09.009","DOIUrl":"10.1016/j.ydbio.2024.09.009","url":null,"abstract":"<div><div>During inner ear development, specification of sensory epithelia requires dynamic regulation of Fgf signaling. In zebrafish, high levels of Fgf are necessary and sufficient to specify the utricular/vestibular macula, whereas the saccular/auditory macula requires a discreet lower level of Fgf. Transcription factors Pax2a and Pax5 act downstream of Fgf to help specify utricular identity, loss of which leads to sporadic extrusion of hair cells from the utricular macula. The mechanism for utricular instability is not clear but is potentially related to reduced expression of <em>cdh1/Ecad</em> caused by disruption of <em>pax2a</em>. Here we find that utricular hair cells in <em>pax2−/−</em> and <em>pax5−/−</em> mutants gradually lose adhesive contact with the macula, leading to ejection of intact hair cells from either the basal or apical surface. The phenotype is far more severe in <em>pax2a−/−</em> mutants and is progressive, resulting in loss of large swaths of the utricular hair cells by 82 hpf. Instability is caused by elevated Fgf signaling in the utricle, as modest reduction of Fgf signaling with a low dose of SU5402 prevents hair cell loss in <em>pax2a−/−</em> mutants. Misexpression of <em>cdh1/Ecad</em> in <em>pax2a−/−</em> mutants partially rescues <em>pax2a−/−</em> mutants. Elevating β-catenin levels by treatment with BIO, or misexpression of a mutant form of β-catenin lacking transcriptional activity but retaining cell adhesion function, fully rescues <em>pax2a−/−</em> mutants. In contrast, Wnt signaling is not required for utricular stability. Thus, Pax2/5 factors serve to counteract the destabilizing effects of elevated Fgf signaling needed to specify utricular identity.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"517 ","pages":"Pages 157-167"},"PeriodicalIF":2.5,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142343360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure, function and formation of the amniote skin pattern 羊膜动物皮纹的结构、功能和形成。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-09-24 DOI: 10.1016/j.ydbio.2024.09.011

Carole Desmarquet-Trin Dinh, Marie Manceau

引用次数: 0

Drivers of vessel progenitor fate define intermediate mesoderm dimensions by inhibiting kidney progenitor specification 血管祖细胞命运的驱动因素通过抑制肾脏祖细胞的规范化来确定中间中胚层的尺寸

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-09-21 DOI: 10.1016/j.ydbio.2024.09.008

Elliot A. Perens , Deborah Yelon

{"title":"Drivers of vessel progenitor fate define intermediate mesoderm dimensions by inhibiting kidney progenitor specification","authors":"Elliot A. Perens , Deborah Yelon","doi":"10.1016/j.ydbio.2024.09.008","DOIUrl":"10.1016/j.ydbio.2024.09.008","url":null,"abstract":"<div><div>Proper organ formation depends on the precise delineation of organ territories containing defined numbers of progenitor cells. Kidney progenitors reside in bilateral stripes of posterior mesoderm that are referred to as the intermediate mesoderm (IM). Previously, we showed that the transcription factors Hand2 and Osr1 act to strike a balance between the specification of the kidney progenitors in the IM and the vessel progenitors in the laterally adjacent territory. Recently, the transcription factor Npas4l – an early and essential driver of vessel and blood progenitor formation – was shown to inhibit kidney development. Here we demonstrate how kidney progenitor specification is coordinated by <em>hand2</em>, <em>osr1</em>, and <em>npas4l</em>. We find that <em>npas4l</em> and the IM marker <em>pax2a</em> are transiently co-expressed in the posterior lateral mesoderm, and <em>npas4l</em> is necessary to inhibit IM formation. Consistent with the expression of <em>npas4l</em> flanking the medial and lateral sides of the IM, our findings suggest roles for <em>npas4l</em> in defining the IM boundaries at each of these borders. At the lateral IM border, <em>hand2</em> promotes and <em>osr1</em> inhibits the formation of <em>npas4l</em>-expressing lateral vessel progenitors, and <em>hand2</em> requires <em>npas4l</em> to inhibit IM formation and to promote vessel formation. Meanwhile, <em>npas4l</em> appears to have an additional role in suppressing IM fate at the medial border: <em>npas4l</em> loss-of-function enhances <em>hand2</em> mutant IM defects and results in excess IM generated outside of the lateral <em>hand2</em>-expressing territory. Together, our findings reveal that establishment of the medial and lateral boundaries of the IM requires inhibition of kidney progenitor specification by the neighboring drivers of vessel progenitor fate.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"517 ","pages":"Pages 126-139"},"PeriodicalIF":2.5,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142281773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0