Developmental biology最新文献

{"title":"Midkine-a interacts with Ptprz1b to regulate neural plate convergence and midline formation in the developing zebrafish hindbrain","authors":"Yao Le ,&nbsp;Kavitha Rajasekhar ,&nbsp;Tricia Y.J. Loo ,&nbsp;Timothy E. Saunders ,&nbsp;Thorsten Wohland ,&nbsp;Christoph Winkler","doi":"10.1016/j.ydbio.2025.02.004","DOIUrl":"10.1016/j.ydbio.2025.02.004","url":null,"abstract":"<div><div>A midline in the developing central nervous system allows symmetric distribution of neural progenitors that later establish functional, bilaterally symmetric neural circuits. In the zebrafish hindbrain, a midline forms early during neurulation as a result of coordinated cell convergence and midline-crossing cell divisions (C-divisions). These processes are controlled by the Wnt/planar cell polarity (PCP) pathway that positions progenitors close to a presumptive midline to perform C-divisions. Other upstream cues that control the extent of neural plate convergence, however, remain unclear. Midkine (Mdk) and pleiotrophin (Ptn) are structurally related heparin-binding growth factors that are dynamically expressed in the developing hindbrain. We show that two zebrafish Mdks, Mdka and Mdkb, as well as Ptn interact with distinct affinities <em>in vivo</em> with the protein tyrosine phosphatase receptor Ptprz1b. Zebrafish <em>mdka</em> and <em>ptprz1b</em> mutants exhibit impaired neural plate convergence along with misplaced C-divisions, defective cell polarity and transiently duplicated midlines. These defects are absent in <em>mdka</em>; <em>mdkb</em> double mutants suggesting antagonistic roles of Mdka and Mdkb to coordinate convergence and C-divisions. Overexpression of <em>Drosophila</em> Prickle, a key component of the Wnt/PCP pathway, rescued the midline duplications in <em>mdka</em> and <em>ptprz1b</em> mutants that exhibited significantly reduced levels of <em>prickle pk1b, pk2a,</em> and <em>pk2b</em> expression. Ptprz1b overexpression, on the other hand, up-regulated <em>pk2a</em> transcription. Our findings therefore suggest roles for Mdka, Mdkb and Ptprz1b in coordinating neural plate convergence, neural progenitor positioning and midline formation by controlling the levels of <em>prickle</em> expression.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"521 ","pages":"Pages 52-74"},"PeriodicalIF":2.5,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A critical genetic interaction between Gemin3/Ddx20 and translation initiation factor NAT1/eIF4G2 drives development","authors":"Rebecca Cacciottolo ,&nbsp;Ruben J. Cauchi","doi":"10.1016/j.ydbio.2025.02.003","DOIUrl":"10.1016/j.ydbio.2025.02.003","url":null,"abstract":"<div><div>Gemin3 (Gem3) or DEAD-box RNA helicase 20 (Ddx20) has been mostly implicated in the assembly of spliceosomal small nuclear ribonucleoproteins (snRNPs) as part of the SMN-Gemins complex. Nonetheless, several studies have hinted at its participation in diverse snRNP-independent activities. Here, we utilised a narrow unbiased genetic screen to discover novel Gem3 interactors in <em>Drosophila</em> with the aim of gaining better insights on its function <em>in vivo</em>. Through this approach, we identified a novel genetic interaction between <em>Gem3</em> and <em>NAT1</em>, which encodes the <em>Drosophila</em> orthologue of translational regulator eIF4G2. Despite lack of a physical association, loss of <em>NAT1</em> function was found to downregulate <em>Gem3</em> mRNA levels. Extensive convergence in transcriptome alterations downstream of <em>Gem3</em> and <em>NAT1</em> silencing further supports a functional relationship between these factors in addition to showing a requirement for both in actin cytoskeleton organisation and organism development, particularly neurodevelopment. In confirmation, flies with either <em>Gem3</em> or <em>NAT1</em> depletion exhibited brain growth defects and reduced muscle contraction. Severe delays in developmental progression were also observed in a newly generated Gem3 hypomorphic mutant. Our data linking Gemin3 to a key component of the translational machinery support an emerging role for Gemin3 in translation that is also critical during organism development.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"521 ","pages":"Pages 37-51"},"PeriodicalIF":2.5,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the differentiation potential of EomesPOS mouse trophoblast cells in mid-gestation","authors":"Avery J. McGinnis,&nbsp;Megan E. Cull,&nbsp;Nichole T. Peterson,&nbsp;Matthew K. Tang,&nbsp;Bryony V. Natale,&nbsp;David R.C. Natale","doi":"10.1016/j.ydbio.2025.02.002","DOIUrl":"10.1016/j.ydbio.2025.02.002","url":null,"abstract":"<div><div>Mouse trophoblast stem (mTS) cells can be derived from the blastocyst or extraembryonic ectoderm as late as embryonic day (E) 6.5 and when cultured <em>in vitro,</em> can differentiate to all trophoblast subtypes of the mature placenta. Expression of the T-box transcription factor, <em>Eomes</em>, is required for the maintenance of, and used to identify mTS cells. During development, <em>Eomes</em> is restricted to the ExE and, by E7.5, to the chorion, after which its expression declines. The placental junctional zone and labyrinth layers are thought to develop exclusively from the ectoplacental cone and chorion, respectively. While it is well established that mTS cells express <em>Eomes in vitro</em>, it is unknown if Eomes-positive (Eomes^POS) trophoblast that reside in the chorion after E6.5 are restricted in their developmental potential to the labyrinth layer <em>in vivo</em>. This study utilized a lineage tracing technique to evaluate the <em>in vivo</em> differentiation of Eomes^POS trophoblast. Using an Ai6 reporter mouse crossed with a tamoxifen-inducible Eomes-Cre-ERT2 mouse, Cre was activated from E7.5 to E9.5, permanently marking all Eomes^POS trophoblast and daughter cells with the ZsGreen fluorescent protein. This approach was complemented with immunofluorescence staining to assess how the Eomes^POS trophoblast had contributed to the differentiated trophoblast population within the placenta by E17.5. Importantly, the results show that daughter cells of Eomes^POS trophoblast in which Cre was activated, contributed to both placental layers; specifically, spongiotrophoblast and glycogen trophoblast within the junctional zone and syncytiotrophoblast and sinusoidal trophoblast giant cells within the labyrinth. This confirms that Eomes^POS trophoblast maintain the capacity to contribute to both placental layers <em>in vivo</em> and do so after E7.5. This study expands our understanding of trophoblast differentiation <em>in vivo</em> and may prove useful in assessing how Eomes^POS trophoblast contribute placental development later in gestation and in the context of placental pathology, where Eomes expression has been reported.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"521 ","pages":"Pages 75-84"},"PeriodicalIF":2.5,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143373743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Slit-independent guidance of longitudinal axons by Drosophila Robo3","authors":"Abigail Carranza,&nbsp;LaFreda J. Howard,&nbsp;Haley E. Brown,&nbsp;Ayawovi Selom Ametepe,&nbsp;Timothy A. Evans","doi":"10.1016/j.ydbio.2025.01.017","DOIUrl":"10.1016/j.ydbio.2025.01.017","url":null,"abstract":"<div><div><em>Drosophila</em> Robo3 is a member of the evolutionarily conserved Roundabout (Robo) receptor family and one of three <em>Drosophila</em> Robo paralogs. During embryonic ventral nerve cord development, Robo3 does not participate in canonical Slit-dependent midline repulsion, but instead regulates the formation of longitudinal axon pathways at specific positions along the medial-lateral axis. Longitudinal axon guidance by Robo3 is hypothesized to be Slit dependent, but this has not been directly tested. Here we create a series of Robo3 variants in which the N-terminal Ig1 domain is deleted or modified, in order to characterize the functional importance of Ig1 and Slit binding for Robo3's axon guidance activity. We show that Robo3 requires its Ig1 domain for interaction with Slit and for proper axonal localization in embryonic neurons, but deleting Ig1 from Robo3 only partially disrupts longitudinal pathway formation. Robo3 variants with modified Ig1 domains that cannot bind Slit retain proper localization and fully rescue longitudinal axon guidance. Our results indicate that Robo3 guides longitudinal axons independently of Slit, and that sequences both within and outside of Ig1 contribute to this Slit-independent activity.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"521 ","pages":"Pages 14-27"},"PeriodicalIF":2.5,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143254984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Avian bioresources for developmental biology: Chicken and quail resources in the United Kingdom, France, and Japan","authors":"Lindsay Henderson ,&nbsp;Yuya Okuzaki ,&nbsp;Christophe Marcelle ,&nbsp;Mike J. McGrew ,&nbsp;Ken-ichi Nishijima","doi":"10.1016/j.ydbio.2025.02.001","DOIUrl":"10.1016/j.ydbio.2025.02.001","url":null,"abstract":"<div><div>Biological resources are essential for research using chickens and quails, particularly in the field of developmental biology. Various lines of chickens and quails with naturally occurring genetic mutations and diverse phenotypes have been developed. Recent advancements in genetic modification techniques, such as using DNA transposons to modify cultured primordial germ cells (PGCs) and lentivirus-mediated transduction of PGCs in vivo, have enabled the creation of several transgenic chicken and quail lines. However, the relatively large body size of chickens and the need to maintain living animals due to the previous lack of reliable frozen stock methods, until the development of cultivating methods of PGCs, has caused a steady decline in the number of available lines globally. Several research facilities maintain chicken and quail lines and provide them for research purposes. This review describes the three main avian resource sites: The National Avian Research Facility at The Roslin Institute in the United Kingdom, Lyon Transgenic Quail Facility (MeLiS) in France, and Avian Bioscience Research Center at Nagoya University in Japan.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"521 ","pages":"Pages 1-13"},"PeriodicalIF":2.5,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143188589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outside Back Cover - Graphical abstract TOC/TOC in double column/Cover image legend if applicable, Bar code, Abstracting and Indexing information","authors":"","doi":"10.1016/S0012-1606(24)00298-7","DOIUrl":"10.1016/S0012-1606(24)00298-7","url":null,"abstract":"","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"518 ","pages":"Page OBC"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143159968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A mathematical model of development shows that cell division, short-range signaling and self-activating gene networks increase developmental noise while long-range signaling and epithelial stiffness reduce it","authors":"Hugo Cano-Fernández ,&nbsp;Tazzio Tissot ,&nbsp;Miguel Brun-Usan ,&nbsp;Isaac Salazar-Ciudad","doi":"10.1016/j.ydbio.2024.11.014","DOIUrl":"10.1016/j.ydbio.2024.11.014","url":null,"abstract":"<div><div>The position of cells during development is constantly subject to noise, i.e. cell-level noise. We do not yet fully understand how cell-level noise coming from processes such as cell division or movement leads to morphological noise, i.e. morphological differences between genetically identical individuals developing in the same environment. To address this question we constructed a large ensemble of random genetic networks regulating cell behaviors (contraction, adhesion, etc.) and cell signaling. We simulated them with a general computational model of development, EmbryoMaker. We identified and studied the dynamics, under cell-level noise, of those networks that lead to the development of animal-like morphologies from simple blastula-like initial conditions. We found that growth by cell division is a major contributor to morphological noise. Self-activating gene network loops also amplified cell-level noise into morphological noise while long-range signaling and epithelial stiffness tended to reduce morphological noise.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"518 ","pages":"Pages 85-97"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PRMT3 gene expression and methylation levels in arrested embryos: Implications for developmental arrest defects","authors":"Wuwen Zhang ,&nbsp;Shifeng Li ,&nbsp;Kai Li ,&nbsp;Ningyu Sun ,&nbsp;Rong Lv ,&nbsp;Jie Ma ,&nbsp;Ping Yin ,&nbsp;Guoqing Tong ,&nbsp;Yuanyuan Chen ,&nbsp;Lu Lu ,&nbsp;Yun Li ,&nbsp;Yuanyuan Wu ,&nbsp;Hua Yan","doi":"10.1016/j.ydbio.2025.01.018","DOIUrl":"10.1016/j.ydbio.2025.01.018","url":null,"abstract":"<div><div>Embryos generated through <em>in vitro</em> fertilization (IVF) frequently experience developmental arrests or blocks, which significantly reduces the success rate of IVF therapy. Recent studies have shown that the protein arginine methylase 3 (<em>PRMT3</em>) plays a crucial role in the regulating of gene expression during early embryonic development. However, the exact regulatory mechanisms of <em>PRMT3</em> involved in early embryonic development are still unclear. In this study, we used discarded arrested and polyspermic embryos from IVF for experiments, employing confocal techniques and qRT-PCR to examine <em>PRMT3</em> expression and changes in H4R3me2a methylation during various stages of early development. Furthermore, <em>PRMT3</em> was re-expressed in the arrested embryos to observe their subsequent development. Our findings revealed that <em>PRMT3</em> nucleic acid and protein were significantly lower in arrested embryos than in control embryos (<em>P</em> &lt; 0.05). Additionally, methylation levels of H4R3me2a were significantly lower in arrested embryos (<em>P</em> &lt; 0.05). Re-expression of <em>PRMT3</em> could partially rescue embryos that are developmentally arrested, and even a few arrested embryos have the potential to develop into morula or blastocysts. In summary, the reduction or deletion of <em>PRMT</em>3 gene in early embryo may lead to developmental arrested defects. Therefore, it is crucial to regulate the expression and functioning of <em>PRMT3</em> for the proper development of early embryos, and further research is required to investigate potential therapeutic interventions for embryonic development arrest <em>in vitro</em>.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"520 ","pages":"Pages 264-271"},"PeriodicalIF":2.5,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluid secretion and luminal pressure control lateral branching morphogenesis in the embryonic avian lung","authors":"Shelby R. Mohr-Allen ,&nbsp;Jason P. Gleghorn ,&nbsp;Victor D. Varner","doi":"10.1016/j.ydbio.2025.01.016","DOIUrl":"10.1016/j.ydbio.2025.01.016","url":null,"abstract":"<div><div>During lung development, the embryonic airway originates as a wishbone-shaped epithelial tube, which undergoes a series of branching events to build the bronchial tree. This process depends crucially on cell proliferation and is thought to involve distinct branching modes: lateral branching, wherein daughter branches emerge along the length of a parent branch, and bifurcations, wherein the tip of a parent branch splits to form two new daughter branches. The developing airway is fluid-filled, and previous studies have shown that altered luminal pressure can influence rates of branching morphogenesis. However, it is not clear if altered tissue mechanics influence patterns of proliferation along the embryonic airway epithelium nor if individual branching modes are affected differently by changes in luminal pressure. Here, we focused on mechanisms of lateral branching and used as a model system the embryonic avian lung, which forms exclusively via this branching mode during early development. We used microinjected fluid droplets or pharmacological modulators of fluid secretion to alter luminal fluid pressure either locally or globally within cultured embryonic lungs. Somewhat surprisingly, we found both local and global increases in luminal pressure to suppress the formation of new lateral branches while also promoting increased epithelial proliferation. In a consistent manner, decreased luminal pressure led to an increase in lateral branching morphogenesis. Morphometric analysis of airway branching patterns revealed that altered luminal pressure shifts the overall branching program, rather than simply changing rates of morphogenesis. Taken together, these results highlight the importance of mechanical forces during airway branching and suggest that different branching modes may be affected differently by luminal fluid pressure.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"520 ","pages":"Pages 251-263"},"PeriodicalIF":2.5,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143051968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual role of Xenopus Odf2 in multiciliated cell patterning and differentiation","authors":"Aude Nommick ,&nbsp;Alexandre Chuyen ,&nbsp;Raphael Clément ,&nbsp;Virginie Thomé ,&nbsp;Fabrice Daian ,&nbsp;Olivier Rosnet ,&nbsp;Fabrice Richard ,&nbsp;Nicolas Brouilly ,&nbsp;Etienne Loiseau ,&nbsp;Camille Boutin ,&nbsp;Laurent Kodjabachian","doi":"10.1016/j.ydbio.2025.01.014","DOIUrl":"10.1016/j.ydbio.2025.01.014","url":null,"abstract":"<div><div>In developing tissues, the number, position, and differentiation of cells must be coordinately controlled to ensure the emergence of physiological function. The epidermis of the <em>Xenopus</em> embryo contains thousands of uniformly distributed multiciliated cells (MCCs), which grow hundreds of coordinately polarized cilia that beat vigorously to generate superficial water flow. Using this model, we uncovered a dual role for the conserved centriolar component Odf2, in MCC apical organization at the cell level, and in MCC spatial distribution at the tissue level. Like in other species, <em>Xenopus</em> Odf2 localized to the basal foot of basal bodies. Consistently, Odf2 morpholino-mediated knockdown impaired basal foot morphogenesis. Consequently, the rate of microtubule nucleation by Odf2-deficient basal bodies was reduced, leading to cilia disorientation, reduced beating, and ultimately altered flow production across the embryo. Furthermore, we show that Odf2 is required to maintain MCC motility and homotypic repulsion prior to their emergence into the surface layer. Our data suggest that Odf2 promotes MCC spacing via its role in the modulation of cytoplasmic microtubule dynamics. Mathematical simulations confirmed that reduced migration speed alters the spacing order of MCCs. This study provides a striking example of coupling between organizational scales by a unique effector.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"520 ","pages":"Pages 224-238"},"PeriodicalIF":2.5,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143045929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}