实验性缺氧条件下鸡胚胎心脏的蛋白质组学分析

IF 2.5 3区生物学 Q2 DEVELOPMENTAL BIOLOGY

Developmental biology Pub Date : 2025-02-09 DOI:10.1016/j.ydbio.2025.02.006

David Sedmera , Eliska Drobna Krejci , Ondrej Nanka , Adam Eckhardt

{"title":"实验性缺氧条件下鸡胚胎心脏的蛋白质组学分析","authors":"David Sedmera , Eliska Drobna Krejci , Ondrej Nanka , Adam Eckhardt","doi":"10.1016/j.ydbio.2025.02.006","DOIUrl":null,"url":null,"abstract":"<div><div>Investigating prenatal hypoxia is difficult in mammals, as there are confounding factors stemming from maternal adaptations and compensatory mechanisms. We have thus established an avian model of hypoxic incubation (starting after 2 days of normoxia, 15% O<sub>2</sub>, normobaric, until the time of sampling at embryonic day 8) to study embryonic reactions to low oxygen concentration. Our previous studies have shown increased vascularization, oedema, and ventricular wall thinning preceding the lethality at mid-gestation. Analysis of the cardiac proteome after 6 days of hypoxic incubation showed strong upregulation of enzymes involved in anaerobic glycolysis as well as an increase in apoptosis-related proteins, cell adhesion proteins, and secretory activity.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"521 ","pages":"Pages 28-36"},"PeriodicalIF":2.5000,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Proteomic analysis of chick embryonic heart in experimental hypoxia\",\"authors\":\"David Sedmera , Eliska Drobna Krejci , Ondrej Nanka , Adam Eckhardt\",\"doi\":\"10.1016/j.ydbio.2025.02.006\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Investigating prenatal hypoxia is difficult in mammals, as there are confounding factors stemming from maternal adaptations and compensatory mechanisms. We have thus established an avian model of hypoxic incubation (starting after 2 days of normoxia, 15% O<sub>2</sub>, normobaric, until the time of sampling at embryonic day 8) to study embryonic reactions to low oxygen concentration. Our previous studies have shown increased vascularization, oedema, and ventricular wall thinning preceding the lethality at mid-gestation. Analysis of the cardiac proteome after 6 days of hypoxic incubation showed strong upregulation of enzymes involved in anaerobic glycolysis as well as an increase in apoptosis-related proteins, cell adhesion proteins, and secretory activity.</div></div>\",\"PeriodicalId\":11070,\"journal\":{\"name\":\"Developmental biology\",\"volume\":\"521 \",\"pages\":\"Pages 28-36\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2025-02-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Developmental biology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0012160625000363\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"DEVELOPMENTAL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Developmental biology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0012160625000363","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"DEVELOPMENTAL BIOLOGY","Score":null,"Total":0}

引用次数: 0

摘要

在哺乳动物中调查产前缺氧是困难的，因为有来自母体适应和补偿机制的混杂因素。因此，我们建立了禽类低氧孵育模型（从正常缺氧，15% O2，常压2天后开始，直到胚胎第8天采样时），以研究胚胎对低氧浓度的反应。我们之前的研究显示妊娠中期死亡前血管增生、水肿和心室壁变薄。缺氧培养6天后的心脏蛋白质组分析显示，参与厌氧糖酵解的酶明显上调，凋亡相关蛋白、细胞粘附蛋白和分泌活性增加。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Proteomic analysis of chick embryonic heart in experimental hypoxia

查看原文本刊更多论文

Proteomic analysis of chick embryonic heart in experimental hypoxia

Investigating prenatal hypoxia is difficult in mammals, as there are confounding factors stemming from maternal adaptations and compensatory mechanisms. We have thus established an avian model of hypoxic incubation (starting after 2 days of normoxia, 15% O₂, normobaric, until the time of sampling at embryonic day 8) to study embryonic reactions to low oxygen concentration. Our previous studies have shown increased vascularization, oedema, and ventricular wall thinning preceding the lethality at mid-gestation. Analysis of the cardiac proteome after 6 days of hypoxic incubation showed strong upregulation of enzymes involved in anaerobic glycolysis as well as an increase in apoptosis-related proteins, cell adhesion proteins, and secretory activity.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Developmental biology 生物-发育生物学

CiteScore

5.30

自引率

3.70%

发文量

182

审稿时长

1.5 months

期刊介绍： Developmental Biology (DB) publishes original research on mechanisms of development, differentiation, and growth in animals and plants at the molecular, cellular, genetic and evolutionary levels. Areas of particular emphasis include transcriptional control mechanisms, embryonic patterning, cell-cell interactions, growth factors and signal transduction, and regulatory hierarchies in developing plants and animals.