Developmental biology最新文献

{"title":"Botryllus humilis: A promising new ascidian model for aging, stem cell dynamics, and whole-body regeneration","authors":"Arzu Karahan","doi":"10.1016/j.ydbio.2025.10.002","DOIUrl":"10.1016/j.ydbio.2025.10.002","url":null,"abstract":"<div><div>Model organisms are essential in science as they provide systems for studying conserved biological processes. In addition to widely used multicellular and unicellular models, tunicates have emerged as valuable model organisms in various biological fields due to their distinctive characteristics. As chordates with both sexual and asexual reproductive strategies and the capacity for whole-body regeneration (WBR), tunicates provide a unique system for regeneration studies. Although the majority of organisms display varying levels of regenerative ability, only a few can perform WBR; notably, botryllid ascidians (Stolidobranchia) are the sole documented group of chordates with WBR. Tunicates, a subphylum of over 3000 identified marine invertebrate species, are the closest living relatives of vertebrates. Botryllids comprise approximately 160 colonial tunicate species within the genera <em>Botryllus</em> and <em>Botrylloides</em>, less than ten of which have been shown to undergo WBR from vascular tissue. However, it remains unknown whether all botryllids are capable of WBR or not. <em>Botryllus humilis</em> was first observed in New Caledonia and has recently been identified in the Mediterranean Sea. In the present study, the blastogenic cycle and WBR capacity of <em>B. humilis</em> were monitored for the first time following the ablation of all zooids and buds. Two temperature conditions and two staging systems were used to record the blastogenetic cycle. Colonies completed their blastogenic cycle in 7 days at 21 °C and 38 ppt salinity, while this duration shortened to 4 days at 26 °C under the same salinity. Colony age appeared to influence the number of budded zooids during the blastogenic cycle. I also assessed the WBR capacity in <em>B. humilis</em> for the first time by inducing WBR at different stages of the blastogenic cycle. Regeneration was completed within 120–138 h post-surgery, and the first post-regeneration blastogenic cycle was completed within 2 days. This study provides valuable insights into <em>B. humilis</em> as a highly valuable model species for aging, stem cell dynamics, and WBR studies.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"529 ","pages":"Pages 56-65"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145231655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Resilience of the replacing dentition in adult reptiles” [Develop. Biol. 516 (2024) 71–81]","authors":"Joaquin I. Henriquez,&nbsp;Joy M. Richman","doi":"10.1016/j.ydbio.2025.10.012","DOIUrl":"10.1016/j.ydbio.2025.10.012","url":null,"abstract":"","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"529 ","pages":"Page 175"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145344095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Poly(A) probe HCR RNA-FISH specifically marks pyriform nurse cells in the brown anole lizard ovary","authors":"Zoe B. Griffin,&nbsp;Bonnie K. Kircher,&nbsp;Richard R. Behringer","doi":"10.1016/j.ydbio.2025.10.017","DOIUrl":"10.1016/j.ydbio.2025.10.017","url":null,"abstract":"<div><div>Hybridization chain reaction RNA-fluorescent in situ hybridization (HCR RNA-FISH) is a powerful and increasingly used method for visualizing gene expression in cells and tissues. A probe set against polyadenylated RNA (poly(A)) is often used as a positive control for RNA integrity and staining quality. While optimizing this technique in the ovary of the brown anole lizard (<em>Anolis sagrei)</em>, we found that the poly(A) probe produced a strikingly specific and intense signal in pyriform cells, a specialized lizard-specific nurse cell type. This staining pattern was found in both whole-mount samples and paraffin sections, suggesting that poly(A) signal intensity can serve as a robust molecular marker for this cell type. The specific and robust signal facilitated segmentation of volumetric data to create the first 3D models of pyriform cells. We also observed unusually diffuse DAPI staining in pyriform cell nuclei, distinguishing them from surrounding granulosa cells, pointing to possible differences in chromatin structure or nuclear organization. Together, these findings highlight the potential of poly(A) probes used in HCR RNA-FISH not only as a technical control, but also as a tool to selectively label specific cell types with high transcriptional activity or storage of abundant poly(A) transcripts.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"529 ","pages":"Pages 219-226"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145353956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphogenetic maturation of chicken chorioallantoic membrane (CAM) and its disruption by tumor xenografts","authors":"Galym Ismagulov ,&nbsp;Yukie Takahashi ,&nbsp;Eri Ishiyama ,&nbsp;Katsutoshi Ogasawara ,&nbsp;Keiichi Sakai ,&nbsp;Yat Nok Chan ,&nbsp;Ruby Yun-Ju Huang ,&nbsp;Takaichi Fukuda ,&nbsp;Guojun Sheng","doi":"10.1016/j.ydbio.2025.10.008","DOIUrl":"10.1016/j.ydbio.2025.10.008","url":null,"abstract":"<div><div><strong>Chorioallantoic membrane (CAM)</strong> functions as embryonic “lung” in birds/reptiles and forms through fusion of the chorion and allantois, two extraembryonic membranes conserved in amniotic vertebrates. Modified CAM gives rise to the placenta, performing similar roles in mammalian development. Despite its importance, cellular morphogenesis leading to CAM maturation is poorly understood. In particular, it is unclear how mesoderm-derived capillary cells are positioned within the chorionic ectoderm layer in mature CAM. In this work, using a combination of live imaging, histology, immunofluorescence and electron microscopy approaches, we carried out a systematic analysis of chicken CAM morphogenesis from embryonic day 4.5 (D4.5; soon after its initial formation) to D15 (when it reaches its fully mature architecture). We show that CAM chorionic ectoderm establishes physical contact with the acellular soft shell from D7.5. CAM mesoderm-derived capillary vessels shift from a sub-chorionic ectodermal position to an intra-chorionic ectodermal one from D11 onward. This is mediated by the downward movement of a subset of chorionic ectoderm cells (termed <strong>chorionic basal cells</strong>) from D9 through inter-capillary spaces, eventually enveloping all superficial capillaries basally and leading to a seemingly mesoderm-out/ectoderm-in topology. All CAM superficial capillary vessels are covered by a thin layer (&lt;1 μm) of chorionic ectodermal cell (<strong>capillary covering cell</strong>) processes. Another type of superficial chorionic ectodermal cell (<strong>villous cavity cell)</strong> contains numerous apical villi. Detailed immunofluorescence and electron microscopy studies revealed that germ layer organization and epithelial cell polarity were maintained during CAM morphogenesis and maturation. Heterogeneity in CAM ectoderm, mesoderm and endoderm cells was observed. Cell proliferation was seen in all three germ layers during early maturation, but became more prominent in chorionic basal cells during later maturation. CAM-tumor graft data suggested that this mature CAM architecture could be modulated as a result of dynamic host-graft tissue interactions.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"529 ","pages":"Pages 154-166"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145285853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ovary organization and oogenesis in two species of cave-living clitellate annelids from the genus Delaya (Clitellata, Pelodrilidae)","authors":"Piotr Świątek,&nbsp;Łukasz Gajda,&nbsp;Anna Z. Urbisz","doi":"10.1016/j.ydbio.2025.09.021","DOIUrl":"10.1016/j.ydbio.2025.09.021","url":null,"abstract":"&lt;div&gt;&lt;div&gt;Clitellate annelids (Clitellata) are hermaphrodites with gonads localized in specific segments in the anterior body part. Localization of gonads and the structure of the reproductive systems are considered conservative traits of clitellate evolution and are used as crucial features in their taxonomy and in phylogenetic considerations. The study aimed to present the ovary morphology, histology, and ultrastructure in two &lt;em&gt;Delaya&lt;/em&gt; species. The genus &lt;em&gt;Delaya&lt;/em&gt; groups poorly known cave-living clitellate annelids, and their ovary organization and oogenesis are entirely unknown. Moreover, their taxonomic status is under debate. According to recent molecular analyses, &lt;em&gt;Delaya&lt;/em&gt; and two other genera form the family Pelodrilidae, closely related to earthworms. To enhance our understanding of these cave-living animals' reproductive biology and provide new characters that may aid in phylogenetic considerations, the light and electron microscopic techniques were used to study the organization of the ovaries and the course of oogenesis in two species: one from a cave in Greece (&lt;em&gt;Delaya&lt;/em&gt; sp. GR) and the other from a cave in France (&lt;em&gt;Delaya&lt;/em&gt; sp. FR). In both species studied, two pairs of ovaries are located in two consecutive segments – XII and XIII. Each ovary consists of 3–5 functional units. The ovarian units are polarized: their apical parts (attached to the septum) contain oogonia and early meiotic cells, while the broader distal ends contain growing oocytes and nurse cells. Initially, Germline cyst formation in cells (oogonia and early meiotic cells) develop synchronously, forming syncytial cysts in which each cell is connected via a single ring canal to the central cytoplasm (cytophore). Then, during meiotic prophase (in diplotene), synchrony is lost, and it is likely that one cell per cyst begins accumulating nutrients and differentiating into an oocyte. As oocytes detach from the cyst and continue oogenesis as individual cells, the remaining cells stay interconnected, do not grow, and are regarded as nurse cells. Yolk absorption is not completed in the ovary; vitellogenic oocytes are transferred to the ovisacs, where they continue to accumulate nutrients. Ovisacs are paired, long, sac-like structures, extending through several body segments (XII-XV). &lt;em&gt;Delaya&lt;/em&gt; produces mesolecithic eggs with prominent yolk spheres, lipid droplets, and glycogen granules. Only some minor differences were observed between the two studied species. The most notable difference concerns the cytophore shape and volume in cysts connecting nurse cells. In &lt;em&gt;Delaya&lt;/em&gt; sp. FR, the cytophore is reticular and inconspicuous, whereas in &lt;em&gt;Delaya&lt;/em&gt; sp. GR, the cytophore is more prominent and may contain nurse cell nuclei.&lt;/div&gt;&lt;div&gt;The obtained results confirm that the formation of the germline cysts equipped with the cytophore is a conservative phase of oogenesis in clitellates. Morphological observations suggest that in &lt;em&gt;Del","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"529 ","pages":"Pages 13-34"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The challenge of teaching K-12 genetic principles: A new approach emphasizing polygenic traits, gene-environment interactions, and genetic non-essentialism to improve genetics literacy and reduce racial stereotyping","authors":"Jamie R. Shuda ,&nbsp;Valerie G. Butler ,&nbsp;Robert Vary ,&nbsp;Darby W. Sweeney ,&nbsp;Fernando L. Wagner ,&nbsp;Steven A. Farber","doi":"10.1016/j.ydbio.2025.09.020","DOIUrl":"10.1016/j.ydbio.2025.09.020","url":null,"abstract":"<div><div>BioEYES is a K-12 life science outreach program that uses zebrafish to teach Mendelian genetics through hands-on activities. The program has operated for 20+ years, promoting academic equity and fostering scientific literacy for all students. While middle school participants show knowledge gains and improved attitudes about science, ∼50% struggle to master foundational genetic concepts. To address this, the authors compared virtual vs. in-person programming, finding minimal differences in learning outcomes but higher gains on simpler survey questions. Rigorous assessments, however, reveal that many students retain only a basic understanding of genetics. Teaching single-gene inheritance, moreover, may reinforce racial stereotypes through \"biological essentialism.\" To counter this, BioEYES staff will be piloting approaches that focus on human polygenic traits, such as skin color, that is taught alongside a hands-on experiment with a zebrafish pigment mutant. Future efforts emphasize developing curriculum around polygenic traits, gene-environment interactions, and challenging stereotypes about race, which research suggests could improve genetics comprehension while reducing racial stereotyping. We invite community feedback in these efforts to enhance K-12 genetics education strategies.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"529 ","pages":"Pages 35-45"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145184787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential lipid and metabolic profiles of embryonic inner cell mass and trophectoderm","authors":"Thamiris Vieira Marsico ,&nbsp;Andressa Minozzo Oliveira ,&nbsp;Roniele Santana Valente ,&nbsp;Kelly Annes ,&nbsp;Christina Ramires Ferreira ,&nbsp;Mateus José Sudano","doi":"10.1016/j.ydbio.2025.10.016","DOIUrl":"10.1016/j.ydbio.2025.10.016","url":null,"abstract":"<div><div>The initial development of mammalian embryos represents a highly intricate and meticulously orchestrated process. This journey encompasses distinct cellular fate determinations and complex regulatory mechanisms. In the scope of our study, we conducted a comprehensive examination of the lipid and metabolic profiles characterizing these distinct cell populations within bovine embryos. In vitro-produced embryos, were submitted to microsurgery and/or immunosurgery techniques to recover trophectoderm (TE) and inner cell mass (ICM) cells. After different cell type obtaining ICM and TE samples underwent lipid and metabolic profiling utilizing multiple reaction monitoring (MRM) profiling mass spectrometry. We unveiled clear and distinct patterns of metabolites expression, including lipids, with TE cells demonstrating a heightened abundance of a variety classes of lipids, whereas ICM cells exhibited specific abundance increase of amino acids. The substantial presence of amino acids in ICM cells aligns with their pivotal role in orchestrating the development of diverse tissues and organs within the emerging organism. Conversely, TE cells are primarily dedicated to preparations for placentation, maternal recognition, and providing essential support to the ICM throughout the developmental process. In summary, our study furnishes valuable insights into the intricate metabolic dynamics that underlie early embryonic development in bovine embryos. It underscores the unique lipidomic and metabolic signatures of ICM and TE cells, reflecting their respective roles in shaping the developmental trajectory. These findings significantly contribute to our deeper understanding of the molecular mechanisms governing embryonic development, offering potential implications for bovine reproduction and broader insights into mammalian embryology.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"529 ","pages":"Pages 188-199"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145336201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Secreted frizzled-related protein 1a regulates hematopoietic development in a dose-dependent manner","authors":"Amber D. Ide,&nbsp;Kelsey A. Carpenter,&nbsp;Mohamed T. Elaswad,&nbsp;Katherine Opria,&nbsp;Kendersley Marcellin,&nbsp;Carla Gilliland,&nbsp;Stephanie Grainger","doi":"10.1016/j.ydbio.2025.09.019","DOIUrl":"10.1016/j.ydbio.2025.09.019","url":null,"abstract":"<div><div>Hematopoietic stem and progenitor cells (HSPCs) are only generated during embryonic development, and their identity specification, emergence from the floor of the dorsal aorta, and proliferation are all tightly regulated by molecular mechanisms such as signaling cues. Among these, Wnt signaling is crucial for HSPC specification, differentiation, and self-renewal, requiring precise regulation for proper development and homeostasis. Wnt signaling begins when a Wnt ligand binds to cell surface receptors, such as those encoded by the <em>frizzled</em> gene family, activating intracellular pathways that regulate gene expression. Secreted frizzled-related proteins (Sfrps) are known to modulate Wnt signaling, acting as both agonists and antagonists. However, the <em>in vivo</em> roles of Sfrps in HSPC development are not fully understood. Here, we show that Sfrp1a influences zebrafish HSPC development and hematopoietic differentiation in a dose-dependent manner. Sfrp1a loss-of-function animals display an upregulation of canonical Wnt signaling, increased HSPC proliferation, and reduced differentiation into lymphoid and myeloid lineages. Conversely, low-dose overexpression of <em>sfrp1a</em> leads to decreased HSPC numbers and enhanced lymphoid differentiation. High-dose <em>sfrp1a</em> overexpression mimics the loss-of-function phenotype, with elevated canonical Wnt signaling, increased HSPCs, and decreased lymphoid and myeloid differentiation. These results emphasize the importance of dose-dependent Sfrp regulation, paralleling observations in hematopoietic cancers where SFRP1 variants can either promote or inhibit tumor development.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"529 ","pages":"Pages 1-12"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145184991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in histone abundance and post-translational modifications in Rana [Lithobates] catesbeiana tissues during thyroid hormone-dependent metamorphosis","authors":"Haley Kuecks-Winger ,&nbsp;Yassene Mohammed ,&nbsp;Caren C. Helbing ,&nbsp;Anita A. Thambirajah","doi":"10.1016/j.ydbio.2025.10.005","DOIUrl":"10.1016/j.ydbio.2025.10.005","url":null,"abstract":"<div><div>Amphibian metamorphosis is thyroid hormone (TH)-dependent and involves extensive gene expression changes in differentiated tissues that result in organismal remodeling. This is likely to involve changes in the epigenome that are poorly understood. We used tandem mass spectrometry (MS/MS) to investigate global variations in histone abundance and histone post-translational modifications (PTMs) during natural metamorphosis to identify putative epigenomic mediators of TH-mediated postembryonic development. Blood, liver, and tailfin from five <em>Rana (Lithobates) catesbeiana</em> (American bullfrog) individuals were collected from functionally athyroid premetamorphic tadpoles and from tadpoles at metamorphic climax (maximal TH levels). Only blood and liver were collected from five individual <em>R. catesbeiana</em> frogs (suprabasal TH levels), as the tailfin is resorbed by this stage. Nuclei were acid extracted to enrich for basic proteins, including histones and other chromatin-associated proteins. MS/MS data were queried against the <em>R. catesbeiana</em> proteome using open search parameters to capture modified histone peptides and stringent statistical evaluation was performed. The abundance of multiple histone variants and isoforms changed throughout metamorphosis. In particular, histone H1.0 increased over the metamorphic stages in all three tissue with a concurrent decrease in H1.3 in blood and liver. Further, H2A variants H2A.X and macroH2A.1 decreased over metamorphosis in blood while H2A type 2-C, H2A.Z-2 and macroH2A.2 increased in liver. We identified putative changes in histone PTMs by analyzing the abundance of peptides carrying mass shifts corresponding to methylation, acetylation and phosphorylation. Our findings include an increase in H1.3 mono- and dimethylation in blood and liver over metamorphosis and suggest a temporary increase of H3.2 phosphorylation at metamorphic climax in liver. To our knowledge, this is the first non-targeted analysis of histone isoform and variant abundance and PTMs during TH-mediated amphibian metamorphosis. Due to the conserved nature of TH signaling, our findings broaden our understanding of the coordinated variations in histone abundance and PTMs that occur across all histone families to direct the tissue-specific transitions that are critical for TH-driven development in amphibians and other vertebrate species.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"529 ","pages":"Pages 142-153"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A zebrafish rbm24a-GFP knock-in line for monitoring lineage-specific dynamic protein expression and function","authors":"Ziwei Ying ,&nbsp;Yizhuang Zhang ,&nbsp;Audrey Saquet ,&nbsp;Ming Shao ,&nbsp;De-Li Shi ,&nbsp;Raphaëlle Grifone","doi":"10.1016/j.ydbio.2025.11.001","DOIUrl":"10.1016/j.ydbio.2025.11.001","url":null,"abstract":"<div><div>The RNA-binding protein Rbm24 is evolutionarily conserved, and its coding gene displays tissue-specific expression in vertebrates. However, the dynamic localization of this protein in different cell lineages remains elusive. We have generated a zebrafish <em>rbm24a-GFP</em> knock-in line in which endogenous Rbm24a is tagged with GFP, allowing the precise monitoring and systematic characterization of its spatiotemporal expression and subcellular localization during development and in the adult. Rbm24a-GFP not only shows strongly restricted expression in a subset of tissues, but also displays cell type- and stage-specific subcellular localization patterns. The protein mainly localizes in the cytoplasm of lens fiber cells and progenitors of sensory hair cells. It undergoes dynamic cytoplasm to nucleus translocation during differentiation of myoblasts and cardiomyoblasts. We further examined the effectiveness of this knock-in line for inhibiting Rbm24a function. Targeted degradation of Rbm24a-GFP using the zGrad system produces phenotypes of zygotic <em>rbm24a</em> mutants or morphants, with defective heart morphogenesis and disrupted cardiac muscle integrity. Therefore, this line will be particularly useful for understanding Rbm24a-GFP dynamic expression and localization changes under homeostasis and pathological conditions. It also enriches the resource of zebrafish knock-in line and provides a convenient tool for functional study of the protein through degron-mediated conditional degradation.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"529 ","pages":"Pages 292-304"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145488227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}