Developmental biology最新文献_第7页

Automated non-invasive laser speckle imaging of the chick heart rate and extraembryonic blood vessels and their response to Nifedipine and Amlodipine drugs 小鸡心率和胚胎外血管的自动无创激光散斑成像及其对硝苯地平和氨氯地平药物的反应。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-12-13 DOI: 10.1016/j.ydbio.2024.12.005

Carol Readhead , Simon Mahler , Zhenyu Dong , Yuki Sato , Changhuei Yang , Marianne E. Bronner

{"title":"Automated non-invasive laser speckle imaging of the chick heart rate and extraembryonic blood vessels and their response to Nifedipine and Amlodipine drugs","authors":"Carol Readhead , Simon Mahler , Zhenyu Dong , Yuki Sato , Changhuei Yang , Marianne E. Bronner","doi":"10.1016/j.ydbio.2024.12.005","DOIUrl":"10.1016/j.ydbio.2024.12.005","url":null,"abstract":"<div><div>Using our recently developed laser speckle contrast imaging (LSCI) to visualize blood vessels and monitor blood flow noninvasively, we test the utility of the developing chick heart as a functional model for drug screening. To this end, we examined the effects of antihypertensive agents Nifedipine and Amlodipine, belonging to the L-type calcium channel antagonist family, on blood flow visualized noninvasively through the intact shell. Guided by the live view mode, the drugs were injected through the shell and ventral to HH16-19 chick embryos. Our results show a significant reduction in the chick's heart rate, blood flow, and vascular size within 5–20 min after Nifedipine or Amlodipine injection. For moderate Nifedipine concentrations, these parameters returned to initial values within 2–3 h. Nifedipine showed a rapid reduction in heart rate and blood flow dynamics at a concentration ten times lower than Amlodipine. These findings show that our LSCI system can monitor and distinguish the chick heart's response to injected drugs from the same family. This serves as proof-of-concept, paving the way for a rapid, cost-effective, and quantitative test system for screening drugs that affect the cardiovascular system of live chick embryos. Live noninvasive imaging may also provide insights into the development and functioning of the vertebrate heart.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"519 ","pages":"Pages 46-54"},"PeriodicalIF":2.5,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142827911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Congenital heart defects differ following left versus right avian cardiac neural crest ablation 左侧和右侧禽类心脏神经嵴消融术后的先天性心脏缺陷不同。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-12-10 DOI: 10.1016/j.ydbio.2024.12.003

Tatiana Solovieva, Marianne E. Bronner

{"title":"Congenital heart defects differ following left versus right avian cardiac neural crest ablation","authors":"Tatiana Solovieva, Marianne E. Bronner","doi":"10.1016/j.ydbio.2024.12.003","DOIUrl":"10.1016/j.ydbio.2024.12.003","url":null,"abstract":"<div><div>The cardiac neural crest is critical for the normal development of the heart, as its surgical ablation in the chick recapitulates common human congenital heart defects such as ‘Common Arterial Trunk’ and ‘Double Outlet Right Ventricle’ (DORV). While left-right asymmetry is known to be important for heart development, little is known about potential asymmetric differences between right and left cardiac neural folds with respect to heart development. Here, through surgical ablation of either left or right cardiac neural crest, we find that right ablation results in more varied and more severe heart defects. Embryos with Common Arterial Trunk and with missing arteries occurred in right-ablated embryos but were not observed in left-ablated embryos; moreover, embryos with DORV and with misalignment of the arteries were more prevalent following right versus left cardiac crest ablation. In addition, survival of right-ablated embryos was lower than left-ablated embryos. Together, these data raise the intriguing possibility that there may be differences in left versus right cardiac neural crest during heart development.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"519 ","pages":"Pages 30-37"},"PeriodicalIF":2.5,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142817559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and functions of the area opaca of the chick embryo 鸡胚不透明区的发育及功能。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-12-09 DOI: 10.1016/j.ydbio.2024.12.002

Hyung Chul Lee , Yara Fadaili , Claudio D. Stern

{"title":"Development and functions of the area opaca of the chick embryo","authors":"Hyung Chul Lee , Yara Fadaili , Claudio D. Stern","doi":"10.1016/j.ydbio.2024.12.002","DOIUrl":"10.1016/j.ydbio.2024.12.002","url":null,"abstract":"<div><div>Before radial symmetry-breaking of the blastoderm, the chick embryo is distinctly divided into a central area pellucida and a surrounding region, the area opaca. In this review, we focus on the area opaca and its functions. First, we survey current knowledge about how the area opaca is formed during the intrauterine period and how it sets up its initial tissue structure. With the formation of a vascularized mesoderm layer, the area opaca becomes subdivided into an inner area vasculosa and an outer area vitellina, which contribute to the development of extraembryonic membranes: the yolk sac and chorion. Second, we review the various functions of the area opaca during development including supplying nutrients, driving the expansion of the embryo by a specialized population of edge cells, and active, instructive signaling that plays a role in the establishment of embryonic polarity and orchestrates the formation of another extraembryonic tissue, the marginal zone, essential for positioning the first midline structure, the primitive streak, at the beginning of gastrulation.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"519 ","pages":"Pages 13-20"},"PeriodicalIF":2.5,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11785533/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142812672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding a Cell's Fate: How Notch and receptor tyrosine kinase signals specify the Drosophila R7 photoreceptor 解码细胞的命运：Notch和受体酪氨酸激酶信号如何指定果蝇R7光感受器。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-12-07 DOI: 10.1016/j.ydbio.2024.12.001

Ronald A. Arias, Andrew Tomlinson

{"title":"Decoding a Cell's Fate: How Notch and receptor tyrosine kinase signals specify the Drosophila R7 photoreceptor","authors":"Ronald A. Arias, Andrew Tomlinson","doi":"10.1016/j.ydbio.2024.12.001","DOIUrl":"10.1016/j.ydbio.2024.12.001","url":null,"abstract":"<div><div>The process by which the <em>Drosophila</em> R7 photoreceptor is specified has become a classic model for understanding how cell-cell signals direct cell fates. In the R7 precursor cell, both the Notch and receptor tyrosine kinase (RTK) signaling pathways are active, and the information they encode directs the specification of the R7 photoreceptor identity. In this process, Notch performs three distinct functions: it both opposes and promotes the actions of the RTK pathway to specify the photoreceptor fate, and it determines the type of photoreceptor that is specified. The RTK pathway drives transcription of <em>phyl</em> - a gene expression necessary for photoreceptor specification. We show that Notch activity induces transcription of the <em>yan</em> gene which encodes a transcriptional repressor of <em>phyl</em>. This defines an antagonism between the two pathways, with RTK promoting and Notch opposing <em>phyl</em> transcription. We previously showed that Notch activity supplies Sevenless to the R7 precursor to allow the RTK pathway hyperactivation required to overcome the Notch repression, and we now identify the regulation of Yan activity as a site of integration of RTK and Notch signaling pathways. Once the cell is specified as a photoreceptor, the third Notch function then prevents <em>seven-up</em> (<em>svp</em>) transcription. The Svp transcription factor directs the R1/6 photoreceptor fate, and the prevention of its expression ensures the default R7 specification.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"519 ","pages":"Pages 21-29"},"PeriodicalIF":2.5,"publicationDate":"2024-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142799657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR-Cas13d as a molecular tool to achieve targeted gene expression knockdown in chick embryos CRISPR-Cas13d作为在鸡胚胎中实现靶向基因表达敲低的分子工具。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-11-30 DOI: 10.1016/j.ydbio.2024.11.013

Minyoung Kim , Erica J. Hutchins

{"title":"CRISPR-Cas13d as a molecular tool to achieve targeted gene expression knockdown in chick embryos","authors":"Minyoung Kim , Erica J. Hutchins","doi":"10.1016/j.ydbio.2024.11.013","DOIUrl":"10.1016/j.ydbio.2024.11.013","url":null,"abstract":"<div><div>The chick embryo is a classical model system commonly used in developmental biology due to its amenability to gene perturbation experiments. Pairing this powerful model organism with cutting-edge technology can significantly expand the range of experiments that can be performed. Recently, the CRISPR-Cas13d system has been successfully adapted for use in zebrafish, medaka, killifish, and mouse embryos to achieve targeted gene expression knockdown. Despite its success in other animal models, no prior study has explored the potential of CRISPR-Cas13d in the chick. Here, we present an adaptation of the CRISPR-Cas13d system to achieve targeted gene expression knockdown in the chick embryo. As proof-of-principle, we demonstrate the knockdown of PAX7, an early neural crest marker. Application of this adapted CRISPR-Cas13d technique resulted in effective knockdown of PAX7 expression and function, comparable to knockdown achieved by translation-blocking morpholino. CRISPR-Cas13d complements preexisting knockdown tools such as CRISPR-Cas9 and morpholinos, thereby expanding the experimental potential and versatility of the chick model system.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"519 ","pages":"Pages 5-12"},"PeriodicalIF":2.5,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Semaphorin 3A repulsion directs the caudal projection of pioneer longitudinal axons in the developing chicken brain 信号蛋白3A排斥力指导发育中的鸡脑先锋纵向轴突的尾侧投射

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-11-28 DOI: 10.1016/j.ydbio.2024.11.012

Kerry-lyn Riley , Susanne Dietrich , Frank R. Schubert

{"title":"Semaphorin 3A repulsion directs the caudal projection of pioneer longitudinal axons in the developing chicken brain","authors":"Kerry-lyn Riley , Susanne Dietrich , Frank R. Schubert","doi":"10.1016/j.ydbio.2024.11.012","DOIUrl":"10.1016/j.ydbio.2024.11.012","url":null,"abstract":"<div><div>The medial longitudinal fasciculus (MLF) is the first axon tract to develop in the ventral vertebrate brain. It originates in the diencephalon and projects caudally into the spinal cord, pioneering the path for later developing axons. Previous anatomical and expression analyses in the chicken suggested Semaphorin 3 A (Sema3A) as the candidate to repel the amniote MLF from the forebrain. However, studies in the zebrafish implicated a distantly related semaphorin with a role in axon fasciculation, not guidance. Thus, the mechanism accounting for the caudal projection of the MLF remains unclear.</div><div>Here we show that misexpression of Sema3A or grafting of Sema3A-expressing cells into the path of the MLF diverts the axons or blocks their outgrowth in chicken embryos. In vitro, Sema3A exposure resulted in the collapse of MLF growth cones. A dominant-negative approach or siRNA to interfere with the function of the Sema3A receptor Neuropilin1 allowed MLF axons to project rostrally. Together, this suggests that Sema3a repulsion directs the caudal extension of the MLF to pioneer the ventral longitudinal tract.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"518 ","pages":"Pages 77-84"},"PeriodicalIF":2.5,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142757418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A conditional smoothened (smo) allele on an inbred C57BL/6J genetic background has a hypomorphic smo mutant phenotype 在近交系 C57BL/6J 遗传背景上，一个条件性 Smoothened（Smo）等位基因具有低形态 Smo 突变体表型。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-11-26 DOI: 10.1016/j.ydbio.2024.11.010

Scott Houghtaling , Sean K. Gombart , Tzu-Hua Ho , Grace Huang , David R. Beier

引用次数: 0

Disease susceptibility implications of preferential inactivation of the paternal X chromosome in extraembryonic endoderm of the mouse 小鼠胚胎外内胚层父系X染色体优先失活对疾病易感性的影响。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-11-26 DOI: 10.1016/j.ydbio.2024.11.011

Virginia E. Papaioannou

{"title":"Disease susceptibility implications of preferential inactivation of the paternal X chromosome in extraembryonic endoderm of the mouse","authors":"Virginia E. Papaioannou","doi":"10.1016/j.ydbio.2024.11.011","DOIUrl":"10.1016/j.ydbio.2024.11.011","url":null,"abstract":"<div><div>In the mouse, there is preferential inactivation of the paternally-derived X chromosome in extraembryonic tissues of early embryos, including trophectoderm and primitive endoderm or hypoblast. Although derivatives of these tissue have long been considered to be purely extraembryonic in nature, recent studies have shown that hypoblast-derived cells of the ‘extraembryonic’ visceral endoderm make a substantial cellular contribution to the definitive gut of the fetus. This raises questions about the eventual fate of these cells in the adult and potential disease implications due to the skewed inactivation of the paternally derived X in females heterozygous for X-linked mutations. Similar lineage studies of this tissue have not yet been done in human embryos but differences in the pattern of X chromosome inactivation between mouse and humans indicates that preferential X inactivation will not be an issue in human embryos. Nonetheless, comparisons between mouse and human will be important because of the widespread use of the mouse as a model system for study of genetics, development and disease.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"519 ","pages":"Pages 1-4"},"PeriodicalIF":2.5,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142750208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatiotemporal characterization of cyclooxygenase pathway enzymes during vertebrate embryonic development 脊椎动物胚胎发育过程中环氧化酶通路酶的时空表征

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-11-22 DOI: 10.1016/j.ydbio.2024.11.009

Tess A. Leathers, Raneesh Ramarapu, Crystal D. Rogers

{"title":"Spatiotemporal characterization of cyclooxygenase pathway enzymes during vertebrate embryonic development","authors":"Tess A. Leathers, Raneesh Ramarapu, Crystal D. Rogers","doi":"10.1016/j.ydbio.2024.11.009","DOIUrl":"10.1016/j.ydbio.2024.11.009","url":null,"abstract":"<div><div>Vertebrate development is regulated by several complex well-characterized morphogen signaling pathways, transcription factors, and structural proteins, but less is known about the enzymatic pathways that regulate early development. We have identified that factors from the inflammation-mediating cyclooxygenase (COX) signaling pathway are expressed at early stages of development in avian embryos. Using <em>Gallus gallus</em> (chicken) as a research model, we characterized the spatiotemporal expression of a subset of genes and proteins in the COX pathway during early neural development stages. Specifically, here we show expression patterns of COX-1, COX-2, and microsomal prostaglandin E synthase-2 (mPGES-2) as well as the genes encoding these enzymes (<em>PTGS1</em>, <em>PTGS2</em>, and <em>PTGES-2</em>). Unique expression patterns of individual players within the COX pathway suggest that they may play non-canonical/non-traditional roles in the embryo compared to their roles in the adult. Future work should examine the function of the COX pathway in tissue specification and morphogenesis and determine if these expression patterns are conserved across species.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"518 ","pages":"Pages 61-70"},"PeriodicalIF":2.5,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142701340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beyond the heat shock pathway: Heat stress responses in Drosophila development 超越热休克途径：果蝇发育过程中的热应激反应

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-11-16 DOI: 10.1016/j.ydbio.2024.11.003

Julia R. Gibbs, Christian Mei, Zeba Wunderlich

{"title":"Beyond the heat shock pathway: Heat stress responses in Drosophila development","authors":"Julia R. Gibbs, Christian Mei, Zeba Wunderlich","doi":"10.1016/j.ydbio.2024.11.003","DOIUrl":"10.1016/j.ydbio.2024.11.003","url":null,"abstract":"<div><div>Heat stress has broad effects on an organism and is an inevitable part of life. Embryos face a particular challenge when faced with heat stress – the intricate molecular processes that pattern the embryo can all be affected by heat, and the embryo lacks some of the strategies that adults can use to manage or avoid heat stress. We use <em>Drosophila melanogaster</em> as a model, as insects are capable of developing normally under a wide range of temperatures and are exposed to daily temperature swings as they develop. Research has focused on the heat shock pathway and the transcription of heat shock proteins as the main response to heat and heat damage. This review explores embryonic heat responses beyond the heat shock pathway. We examine the effects of heat from a biochemical standpoint, as well as highlighting other mechanisms of heat stress regulation, such as miRNA activity or other signaling pathways. We discuss how different elements of the heat stress response must be coordinated across the embryo to enable development under a wide range of temperatures. Studying heat stress in <em>Drosophila melanogaster</em> can be a powerful lens into how developmental systems ensure robustness to environmental factors.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"518 ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142667342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0