Developmental biology最新文献

{"title":"Pax3/7 gene function in Oikopleura dioica supports a neuroepithelial-like origin for its house-making Fol territory","authors":"David Lagman ,&nbsp;Anthony Leon ,&nbsp;Nadia Cieminska ,&nbsp;Wei Deng ,&nbsp;Marios Chatzigeorgiou ,&nbsp;Simon Henriet ,&nbsp;Daniel Chourrout","doi":"10.1016/j.ydbio.2024.08.012","DOIUrl":"10.1016/j.ydbio.2024.08.012","url":null,"abstract":"<div><p>Larvacean tunicates feature a spectacular innovation not seen in other animals - the trunk oikoplastic epithelium (OE). This epithelium produces a house, a large and complex extracellular structure used for filtering and concentrating food particles. Previously we identified several homeobox transcription factor genes expressed during early OE patterning. Among these are two <em>Pax3/7</em> copies that we named <em>pax37A</em> and <em>pax37B</em>. The vertebrate homologs, <em>PAX3</em> and <em>PAX7</em> are involved in developmental processes related to neural crest and muscles. In the ascidian tunicate <em>Ciona intestinalis</em>, <em>Pax3/7</em> plays a role in the development of cells deriving from the neural plate border, including trunk epidermal sensory neurons and tail nerve cord neurons, as well as in the neural tube closure. Here we have investigated the roles of <em>Oikopleura dioica pax37A</em> and <em>pax37B</em> in the development of the OE, by using CRISPR-Cas9 mutant lines and analyzing scRNA-seq data from wild-type animals. We found that <em>pax37B</em> but not <em>pax37A</em> is essential for the differentiation of cell fields that produce the food concentrating filter of the house: the anterior Fol, giant Fol and Nasse cells. Trajectory analysis supported a neuroepithelial-like or a preplacodal ectoderm transcriptional signature in these cells. We propose that the highly specialized secretory epithelial cells of the Fol region either maintained or evolved neuroepithelial features. This is supported by a fragmented gene regulatory network involved in their development that also operates in ascidian epidermal neurons.</p></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"516 ","pages":"Pages 207-220"},"PeriodicalIF":2.5,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0012160624002173/pdfft?md5=64a013109c29b07527c91d9aace535e5&pid=1-s2.0-S0012160624002173-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142055207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The DNA methyltransferase DMAP1 is required for tissue maintenance and planarian regeneration","authors":"Salvador Rojas ,&nbsp;Paul G. Barghouth ,&nbsp;Peter Karabinis ,&nbsp;Néstor J. Oviedo","doi":"10.1016/j.ydbio.2024.08.007","DOIUrl":"10.1016/j.ydbio.2024.08.007","url":null,"abstract":"<div><p>The precise regulation of transcription is required for embryonic development, adult tissue turnover, and regeneration. Epigenetic modifications play a crucial role in orchestrating and regulating the transcription of genes. These modifications are important in the transition of pluripotent stem cells and their progeny. Methylation, a key epigenetic modification, influences gene expression through changes in DNA methylation. Work in different organisms has shown that the DNA methyltransferase-1-associated protein (DMAP1) may associate with other molecules to repress transcription through DNA methylation. Thus, DMAP1 is a versatile protein implicated in a myriad of events, including pluripotency maintenance, DNA damage repair, and tumor suppression. While DMAP1 has been extensively studied <em>in vitro</em>, its complex regulation in the context of the adult organism remains unclear. To gain insights into the possible roles of DMAP1 at the organismal level, we used planarian flatworms that possess remarkable regenerative capabilities driven by pluripotent stem cells called neoblast. Our findings demonstrate the evolutionary conservation of DMAP1 in the planarian <em>Schmidtea mediterranea</em>. Functional disruption of DMAP1 through RNA interference revealed its critical role in tissue maintenance, neoblast differentiation, and regeneration in <em>S. mediterranea</em>. Moreover, our analysis unveiled a novel function for DMAP1 in regulating cell death in response to DNA damage and influencing the expression of axial polarity markers. Our findings provide a simplified paradigm for studying DMAP1's function in adult tissues.</p></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"516 ","pages":"Pages 196-206"},"PeriodicalIF":2.5,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142046451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hypoxia-sonic hedgehog axis as a driver of primitive hematopoiesis development and evolution in cavefish","authors":"Corine M. van der Weele,&nbsp;Katrina C. Hospes,&nbsp;Katherine E. Rowe,&nbsp;William R. Jeffery","doi":"10.1016/j.ydbio.2024.08.008","DOIUrl":"10.1016/j.ydbio.2024.08.008","url":null,"abstract":"<div><p>The teleost <em>Astyanax mexicanus</em> consists of surface dwelling (surface fish) and cave dwelling (cavefish) forms. Cavefish have evolved in subterranean habitats characterized by reduced oxygen levels (hypoxia) and exhibit a subset of phenotypic traits controlled by increased Sonic hedgehog (Shh) signaling along the embryonic midline. The enhancement of primitive hematopoietic domains, which are formed bilaterally in the anterior and posterior lateral plate mesoderm, are responsible for the development of more larval erythrocytes in cavefish relative to surface fish. In this study, we determine the role of hypoxia and Shh signaling in the development and evolution of primitive hematopoiesis in cavefish. We show that hypoxia treatment during embryogenesis increases primitive hematopoiesis and erythrocyte development in surface fish. We also demonstrate that upregulation of Shh midline signaling by the Smoothened agonist SAG increases primitive hematopoiesis and erythrocyte development in surface fish, whereas Shh downregulation via treatment with the Smoothened inhibitor cyclopamine decreases these traits in cavefish. Together these results suggest that hematopoietic enhancement is regulated by hypoxia and Shh signaling. Lastly, we demonstrate that hypoxia enhances expression of Shh signaling along the midline of surface fish embryos. We conclude that hypoxia-mediated Shh plasticity may be a driving force for the adaptive evolution of primitive hematopoiesis and erythrocyte development in cavefish.</p></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"516 ","pages":"Pages 138-147"},"PeriodicalIF":2.5,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142021438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maternal Spargel/dPGC-1 is critical for embryonic development and influences chorion gene amplification via Cyclin E activity","authors":"Md Shah Jalal,&nbsp;Atanu Duttaroy","doi":"10.1016/j.ydbio.2024.08.013","DOIUrl":"10.1016/j.ydbio.2024.08.013","url":null,"abstract":"<div><p>The function of <em>spargel</em>/<em>dPGC-1</em> in <em>Drosophila</em> oogenesis has been unequivocally established. Here, we sought to assess whether Spargel protein or RNA is essential for developmentally competent eggs. The trans-heterozygotic combination of two <em>spargel</em> mutant alleles allowed us to decrease Spargel expression to very low levels. Using this model, we now demonstrated the requirement for Spargel in eggshell patterning and embryonic development, which led us to establish that <em>spargel</em> is a maternal effect gene. Further examination of Spargel's potential mechanism of action in eggshell biogenesis revealed that low levels of Spargel in the adult ovary cause diminished Cyclin E activity, resulting in reduced chorion gene amplification levels, leading to eggshell biogenesis defects. Thus, another novel role for <em>spargel/dPGC-1</em> is exposed whereby, through Cyclin E activity, this conserved transcriptional coactivator regulates the chorion gene amplification process.</p></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"516 ","pages":"Pages 158-166"},"PeriodicalIF":2.5,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142035497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Caenorhabditis elegans protein SOC-3 permits an alternative mode of signal transduction by the EGL-15 FGF receptor","authors":"Claudia S. Rodriguez Torres ,&nbsp;Nicole B. Wicker ,&nbsp;Victória Puccini de Castro ,&nbsp;Mariya Stefinko ,&nbsp;Daniel C. Bennett ,&nbsp;Brooke Bernhardt ,&nbsp;Melissa Garcia Montes de Oca ,&nbsp;Sainabou Jallow ,&nbsp;Katelyn Flitcroft ,&nbsp;Jessica-Jae S. Palalay ,&nbsp;Omar A. Payán Parra ,&nbsp;Yaakov E. Stern ,&nbsp;Michael R. Koelle ,&nbsp;Cindy Voisine ,&nbsp;Ian G. Woods ,&nbsp;Te-Wen Lo ,&nbsp;Michael J. Stern ,&nbsp;Claire C. de la Cova","doi":"10.1016/j.ydbio.2024.08.014","DOIUrl":"10.1016/j.ydbio.2024.08.014","url":null,"abstract":"<div><p>Fibroblast Growth Factors and their receptors (FGFRs) comprise a cell signaling module that can stimulate signaling by Ras and the kinases Raf, MEK, and ERK to regulate animal development and homeostatic functions. In <em>Caenorhabditis elegans,</em> the sole FGFR ortholog EGL-15 acts with the GRB2 ortholog SEM-5 to promote chemoattraction and migration by the sex myoblasts (SMs) and fluid homeostasis by the hypodermis (Hyp7). Cell-specific differences in EGL-15 signaling were suggested by the phenotypes caused by <em>egl-15(n1457),</em> an allele that removes a region of its C-terminal domain (CTD) known to bind SEM-5. To determine how mutations altered EGL-15 activity in the SMs and Hyp7, we used the kinase reporter ERK-KTR to measure activation of the ERK ortholog MPK-1. Consequences of <em>egl-15(n1457)</em> were cell-specific, resulting in loss of MPK-1 activity in the SMs and elevated activity in Hyp7. Previous studies of Hyp7 showed that loss of the CLR-1 phosphatase causes a fluid homeostasis defect termed “Clear” that is suppressed by reduction of EGL-15 signaling, a phenotype termed “Suppressor of Clear” (Soc). To identify mechanisms that permit EGL-15 signaling in Hyp7, we conducted a genetic screen for Soc mutants in the <em>clr-1; egl-15(n1457)</em> genotype. We report the identification of SOC-3, a protein with putative SEM-5-binding motifs and PH and PTB domains similar to DOK and IRS proteins. In combination with the <em>egl-15(n1457)</em> mutation, loss of either <em>soc-3</em>, the GAB1 ortholog <em>soc-1</em>, or the SHP2 ortholog <em>ptp-2</em>, reduced MPK-1 activation. We generated alleles of <em>soc-3</em> to test the requirement for the SEM-5-binding motifs, finding that residue Tyr³⁵⁶ is required for function. We propose that EGL-15-mediated SM chemoattraction relies solely on the direct interaction between SEM-5 and the EGL-15 CTD. In Hyp7, EGL-15 signaling uses two mechanisms: the direct SEM-5 binding mechanism; and an alternative, CTD-independent mechanism involving SOC-3, SOC-1, and PTP-2. This work demonstrates that FGF signaling uses distinct, tissue-specific mechanisms in development, and identifies SOC-3 as a potential adaptor that facilitates Ras pathway activation by FGFR.</p></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"516 ","pages":"Pages 183-195"},"PeriodicalIF":2.5,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142035498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomics, the diversification of mammals, and the evolution of placentation","authors":"Anthony M. Carter","doi":"10.1016/j.ydbio.2024.08.011","DOIUrl":"10.1016/j.ydbio.2024.08.011","url":null,"abstract":"<div><p>When and why did variations in placental structure and function evolve? Such questions cannot be addressed without a reliable version of mammalian phylogeny. Twenty-five years ago, the mammalian tree was reshaped by molecular phylogenetics. Soon it was shown, in contrast to prevailing theories, that the common ancestor of placental mammals had invasive placentation. Subsequently, evolution of many other features of extraembryonic membranes was addressed. This endeavour stimulated research to fill gaps in our knowledge of placental morphology. Last year the mammalian tree was again revised based on a large set of genomic data. With that in mind, this review provides an update on placentation in the nineteen orders of placental mammals, incorporating much recent data. The principal features such as shape, interdigitation, the interhaemal barrier and the yolk sac are summarized in synoptic tables. The evolution of placental traits and its timing is then explored by reference to the revised mammalian tree. Examples are the early appearance of epitheliochorial placentation in the common ancestor of artiodactyls, perissodactyls, pangolins and carnivores (with reversion to invasive forms in the latter) and later refinements such as the binucleate trophoblast cells and placentomes of ruminants. In primates, the intervillous space gradually evolved from the more basic labyrinth whereas trophoblast invasion of the decidua was a late development in humans and great apes. Only seldom can we glimpse the “why” of placental evolution. The best examples concern placental hormones, including some striking examples of convergent evolution such as the chorionic gonadotropins of primates and equids. In concluding, I review current ideas about what drives placental evolution and identify significant gaps in our knowledge of placentation, including several relevant to the evolution of placentation in primates.</p></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"516 ","pages":"Pages 167-182"},"PeriodicalIF":2.5,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0012160624002161/pdfft?md5=fca594ffe0940d477f41ee52d6ecffaf&pid=1-s2.0-S0012160624002161-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142035496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Apoptosis-dependent head development during metamorphosis of the cnidarian Hydractinia symbiolongicarpus","authors":"Gabriel Krasovec ,&nbsp;Uri Frank","doi":"10.1016/j.ydbio.2024.08.010","DOIUrl":"10.1016/j.ydbio.2024.08.010","url":null,"abstract":"<div><p>Apoptosis is a regulated cell death that depends on caspases. It has mainly been studied as a mechanism for the removal of unwanted cells. However, apoptotic cells can induce fate or behavior changes of their neighbors and thereby participate in development. Here, we address the functions of apoptosis during metamorphosis of the cnidarian <em>Hydractinia symbiolongicarpus</em>. We describe the apoptotic profile during metamorphosis of the larva and identify <em>Caspase3/7a</em>, but no other executioner caspases, as essential for apoptosis in this context. Using pharmacological and genetic approaches, we find that apoptosis is required for normal head development. Inhibition of apoptosis resulted in defects in head morphogenesis. Neurogenesis was compromised in the body column of apoptosis-inhibited animals but there was no effect on the survival or proliferation of stem cells, suggesting that apoptosis is required for cellular commitment rather than for the maintenance of their progenitors. Differential transcriptomic analysis identifies TRAF genes as downregulated in apoptosis-inhibited larvae and functional experiments provide evidence that they are essential for head development. Finally, we find no major role for apoptosis in head regeneration in this animal, in contrast to the significance of apoptosis in <em>Hydra</em> head regeneration.</p></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"516 ","pages":"Pages 148-157"},"PeriodicalIF":2.5,"publicationDate":"2024-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S001216062400215X/pdfft?md5=fcf0b754c07faaf8f452d90a9684ecf1&pid=1-s2.0-S001216062400215X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142008464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolution, development, and regeneration of tooth-like epithelial appendages in sharks","authors":"Ella F. Nicklin ,&nbsp;Karly E. Cohen ,&nbsp;Rory L. Cooper ,&nbsp;Gianna Mitchell ,&nbsp;Gareth J. Fraser","doi":"10.1016/j.ydbio.2024.08.009","DOIUrl":"10.1016/j.ydbio.2024.08.009","url":null,"abstract":"<div><p>Sharks and their relatives are typically covered in highly specialized epithelial appendages embedded in the skin called dermal denticles; ancient tooth-like units (odontodes) composed of dentine and enamel-like tissues. These ‘skin teeth’ are remarkably similar to oral teeth of vertebrates and share comparable morphological and genetic signatures. Here we review the histological and morphological data from embryonic sharks to uncover characters that unite all tooth-like elements (odontodes), including teeth and skin denticles in sharks. In addition, we review the differences between the skin and oral odontodes that reflect their varied capacity for renewal. Our observations have begun to decipher the developmental and genetic shifts that separate these seemingly similar dental units, including elements of the regenerative nature in both oral teeth and the emerging skin denticles from the small-spotted catshark (<em>Scyliorhinus canicula</em>) and other chondrichthyan models. Ultimately, we ask what defines a tooth at both the molecular and morphological level. These insights aim to help us understand how nature makes, replaces and evolves a vast array of odontodes.</p></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"516 ","pages":"Pages 221-236"},"PeriodicalIF":2.5,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0012160624002148/pdfft?md5=94c5bfefcbdba0d1c3e3798e0ac23663&pid=1-s2.0-S0012160624002148-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141999523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Voltage-gated ion channel’s gene expression in the myocardium of embryo and adult chickens","authors":"E.A. Lebedeva ,&nbsp;M.A. Gonotkov ,&nbsp;A.A. Furman ,&nbsp;I.O. Velegzhaninov","doi":"10.1016/j.ydbio.2024.08.005","DOIUrl":"10.1016/j.ydbio.2024.08.005","url":null,"abstract":"<div><p>The functioning of the cardiovascular system is critical for embryo survival. Cardiac contractions depend on the sequential activation of different classes of voltage-gated ion channels. Understanding the fundamental features of these interactions is important for identifying the mechanisms of pathologies development in the myocardium. However, at present there is no consensus on which ion channels are involved in the formation of automaticity in the early embryonic stages. The aim of this study was to elucidate the expression of genes encoding various types of ion channels that are involved in the generation of electrical activity chicken heart at different stages of ontogenesis. We analyzed the expression of 14 genes from different families of ion channels. It was revealed that the expression profiles of ion channel genes change depending on the stages of ontogenesis. The <em>HCN4</em>, <em>CACNA1D</em>, <em>SCN1A</em>, <em>SCN5A</em>, <em>KCNA1</em> genes have maximum expression at the tubular heart stage. In adult, a switch occurs to the higher expression of <em>CACNA1C</em>, <em>KCNH6</em>, <em>RYR</em> and <em>SLC8A1</em> genes. This data correlated with the results obtained by the microelectrode method. It can be assumed that the automaticity of the tubular heart is mainly due to the mechanism of the «membrane–clock» (hyperpolarization-activated current (<em>I</em>_f), Ca²⁺–current L–type (<em>I</em>_CaL), Na⁺–current (<em>I</em>_Na) and the slow component of the delayed rectifier K⁺–current (<em>I</em>_Ks)). Whereas in adult birds, the mechanism for generating electrical impulses is determined by both « membrane– clock» and «Ca²⁺–clock».</p></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"516 ","pages":"Pages 130-137"},"PeriodicalIF":2.5,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141912118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outside Back Cover - Graphical abstract TOC/TOC in double column/Cover image legend if applicable, Bar code, Abstracting and Indexing information","authors":"","doi":"10.1016/S0012-1606(24)00205-7","DOIUrl":"10.1016/S0012-1606(24)00205-7","url":null,"abstract":"","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"515 ","pages":"Page OBC"},"PeriodicalIF":2.5,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141963355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}