Developmental biology最新文献_第7页

Ancient emergence of neuronal heterogeneity in the enteric nervous system of jawless vertebrates 古代无颌脊椎动物肠神经系统中神经元异质性的出现。

IF 2.5 3区生物学

Developmental biology Pub Date : 2025-01-03 DOI: 10.1016/j.ydbio.2024.12.020

Brittany M. Edens, Jason Lin, Marianne E. Bronner

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Outside Back Cover - Graphical abstract TOC/TOC in double column/Cover image legend if applicable, Bar code, Abstracting and Indexing information

IF 2.5 3区生物学

Developmental biology Pub Date : 2025-01-01 DOI: 10.1016/S0012-1606(24)00272-0

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Spatiotemporal control of cell ablation using Ronidazole with Nitroreductase in Drosophila 硝基还原酶Ronidazole对果蝇细胞消融的时空控制。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-12-28 DOI: 10.1016/j.ydbio.2024.12.017

Gary Teeters , Christina E. Cucolo , Sagar N. Kasar , Melanie I. Worley , Sarah E. Siegrist

{"title":"Spatiotemporal control of cell ablation using Ronidazole with Nitroreductase in Drosophila","authors":"Gary Teeters , Christina E. Cucolo , Sagar N. Kasar , Melanie I. Worley , Sarah E. Siegrist","doi":"10.1016/j.ydbio.2024.12.017","DOIUrl":"10.1016/j.ydbio.2024.12.017","url":null,"abstract":"<div><div>The ability to induce cell death in a controlled stereotypic manner has led to the discovery of evolutionary conserved molecules and signaling pathways necessary for tissue growth, repair, and regeneration. Here we report the development of a new method to genetically induce cell death in a controlled stereotypic manner in <em>Drosophila</em>. This method has advantages over other current methods and relies on expression of the <em>E. coli</em> enzyme Nitroreductase (NTR) with exogenous application of the nitroimidazole prodrug, Ronidazole. NTR expression is controlled spatially using the GAL4/UAS system while temporal control of cell death is achieved through timed feeding of Ronidazole supplied in the diet. In cells expressing NTR, Ronidazole is converted to a toxic substance inducing DNA damage and cell death. Caspase cell death is achieved in a range of NTR-expressing cell types with Ronidazole feeding, including epithelial, neurons, and glia. Removing Ronidazole from the diet restores cell death to normal unperturbed levels. Unlike other genetic ablation methods, temporal control is achieved through feeding not temperature, circumventing developmental complications associated with temperature changes. Ronidazole-NTR also requires only two transgenes, a GAL4 driver and <em>UAS-NTR</em>, which is generated as a GFP-NTR fusion allowing for easy setup of large-scale screening of <em>UAS-RNAi</em> lines. Altogether, Ronidazole-NTR provides a new streamlined method for inducing cell death in <em>Drosophila</em> with temperature-independent ON/OFF control.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"520 ","pages":"Pages 31-40"},"PeriodicalIF":2.5,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142906699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Introduction to “Research that Transformed Developmental Biology” 改变发育生物学的研究 "简介。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-12-27 DOI: 10.1016/j.ydbio.2024.12.011

Steven L. Klein Ph.D.

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Teaching students to effectively evaluate scientific evidence and advocate for research in the context of autism spectrum disorder and the neurodiversity movement 在自闭症谱系障碍和神经多样性运动的背景下，教学生有效地评估科学证据并倡导研究。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-12-27 DOI: 10.1016/j.ydbio.2024.12.015

Bridget T. Jacques-Fricke

{"title":"Teaching students to effectively evaluate scientific evidence and advocate for research in the context of autism spectrum disorder and the neurodiversity movement","authors":"Bridget T. Jacques-Fricke","doi":"10.1016/j.ydbio.2024.12.015","DOIUrl":"10.1016/j.ydbio.2024.12.015","url":null,"abstract":"<div><div>Connecting socially relevant topics with biological content can boost student engagement and comprehension. Autism Spectrum Disorder (ASD) is an increasingly prevalent diagnosis with a number of intersecting topic areas between developmental biology and social justice. Here I describe two exercises that I developed to engage students in learning opportunities that link scientific process learning goals with real-world applications. First, students examine scientific research practices and work on connecting scientific evidence with conclusions by evaluating the retracted 1998 article by Andrew Wakefield that falsely linked the measles, mumps and rubella vaccination with the development of ASD. Second, students participate in a role-playing exercise to learn about the multiple viewpoints and perspectives that are involved in determining funding levels for scientific research in the United States, including learning about the neurodiversity movement and its impact on establishing ASD research priorities. By explicitly discussing appropriate scientific practices, analyzing the consequences of scientific misconduct and the spread of misinformation, and demonstrating how students can use their voices and their votes to support science funding, we can prepare students to become knowledgeable, empowered, scientifically literate citizens.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"519 ","pages":"Pages 151-158"},"PeriodicalIF":2.5,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142902696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oogenesis involves a novel nuclear envelop remodeling mechanism in Schmidtea mediterranea 地中海Schmidtea mediterranea的卵发生涉及一种新的核膜重塑机制。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-12-26 DOI: 10.1016/j.ydbio.2024.12.018

Longhua Guo , Fengli Guo , Shasha Zhang , An Zeng , Kexi Yi , Melainia McClain , Claus-D. Kuhn , Tari Parmely , Alejandro Sánchez Alvarado

{"title":"Oogenesis involves a novel nuclear envelop remodeling mechanism in Schmidtea mediterranea","authors":"Longhua Guo , Fengli Guo , Shasha Zhang , An Zeng , Kexi Yi , Melainia McClain , Claus-D. Kuhn , Tari Parmely , Alejandro Sánchez Alvarado","doi":"10.1016/j.ydbio.2024.12.018","DOIUrl":"10.1016/j.ydbio.2024.12.018","url":null,"abstract":"<div><div>The cell nuclei of Ophisthokonts, the eukaryotic supergroup defined by fungi and metazoans, is remarkable in the constancy of their double-membraned structure in both somatic and germ cells. Such remarkable structural conservation underscores common and ancient evolutionary origins. Yet, the dynamics of disassembly and reassembly displayed by Ophisthokont nuclei vary extensively. Besides closed mitosis in fungi and open mitosis in some animals, little is known about the evolution of nuclear envelope remodeling dynamics during oogenesis. Here, we uncovered a novel form of nuclear envelope remodeling as oocytes are formed in the flatworm <em>Schmidtea mediterranea</em>. From zygotene to metaphase II, both nuclear envelope (NE) and peripheral endoplasmic reticulum (ER) expand notably in size, likely involving <em>de novo</em> membrane synthesis. 3-D electron microscopy reconstructions demonstrated that the NE transforms itself into numerous double-membraned vesicles similar in membrane architecture to NE doublets in mammalian oocytes after germinal vesicle breakdown. The vesicles are devoid of nuclear pore complexes and DNA, yet are loaded with nuclear proteins, including a planarian homologue of PIWI, a protein essential for the maintenance of stem cells in this and other organisms. Our data contribute a new model to the canonical view of NE dynamics and suggest important roles of NE remodeling in planarian oogenesis.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"520 ","pages":"Pages 13-20"},"PeriodicalIF":2.5,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142892898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A chitin-binding domain-containing gene is essential for shell development in the mollusc Tritia 一种含有几丁质结合结构域的基因对软体动物的壳发育至关重要。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-12-24 DOI: 10.1016/j.ydbio.2024.12.016

T. Kim Dao, Kailey Ferger , J. David Lambert

引用次数: 0

Post-eclosion growth in the Drosophila ejaculatory duct is driven by Juvenile hormone signaling and is essential for male fertility 果蝇射精管的羽化后生长是由幼年激素信号驱动的，对雄性生殖能力至关重要。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-12-22 DOI: 10.1016/j.ydbio.2024.12.010

Navyashree A. Ramesh, Allison M. Box, Laura A. Buttitta

{"title":"Post-eclosion growth in the Drosophila ejaculatory duct is driven by Juvenile hormone signaling and is essential for male fertility","authors":"Navyashree A. Ramesh, Allison M. Box, Laura A. Buttitta","doi":"10.1016/j.ydbio.2024.12.010","DOIUrl":"10.1016/j.ydbio.2024.12.010","url":null,"abstract":"<div><div>The <em>Drosophila</em> Ejaculatory duct (ED) is a secretory tissue of the somatic male reproductive system. The ED is involved in the secretion of seminal fluid components and ED-specific antimicrobial peptides that aid in fertility and the female post-mating response. The ED is composed of secretory epithelial cells surrounded by a layer of innervated contractile muscle. The ED grows in young adult males during the first 24 h post-eclosion, but the cell cycle status of the ED secretory cells and the role of post-eclosion ED growth have been unexplored. Here, we show that secretory cells of the adult <em>Drosophila</em> ED undergo variant cell cycles lacking mitosis called the endocycle, that lead to an increase in the cell and organ size of the ED post eclosion. The cells largely exit the endocycle by day 3 of adulthood, when the growth of the ED ceases, resulting in a tissue containing cells of ploidies ranging from 8C to 32C. The size of the ED directly correlates with the ploidy of the secretory cells, with additional ectopic endocycles increasing organ size. When endoreplication is compromised in ED secretory cells, it leads to reduced organ size, reduced protein synthesis and compromised fertility. We provide evidence that the growth and endocycling in the young adult male ED is dependent on Juvenile hormone (JH) signaling and we suggest that hormone-induced early adult endocycling is required for optimal fertility and function of the ED tissue. We propose to use the ED as a post-mitotic tissue model to study the role of polyploidy in regulating secretory tissue growth and function.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"519 ","pages":"Pages 122-141"},"PeriodicalIF":2.5,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The transcriptional landscape of the developing chick trigeminal ganglion 发育中的小鸡三叉神经节的转录景观。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-12-22 DOI: 10.1016/j.ydbio.2024.12.013

Carrie E. Leonard , Alec McIntosh , Johena Sanyal , Lisa A. Taneyhill

{"title":"The transcriptional landscape of the developing chick trigeminal ganglion","authors":"Carrie E. Leonard , Alec McIntosh , Johena Sanyal , Lisa A. Taneyhill","doi":"10.1016/j.ydbio.2024.12.013","DOIUrl":"10.1016/j.ydbio.2024.12.013","url":null,"abstract":"<div><div>The trigeminal ganglion is a critical structure in the peripheral nervous system, responsible for transmitting sensations of touch, pain, and temperature from craniofacial regions to the brain. Trigeminal ganglion development depends upon intrinsic cellular programming as well as extrinsic signals exchanged by diverse cell populations. With its complex anatomy and dual cellular origin from cranial placodes and neural crest cells, the trigeminal ganglion offers a rich context for examining diverse biological processes, including cell migration, fate determination, adhesion, and axon guidance. Avian models have, so far, enabled key insights into craniofacial and peripheral nervous system development. Yet the molecular mechanisms driving trigeminal ganglion formation and subsequent nerve growth remain elusive. In this study, we performed RNA-sequencing at multiple stages of chick trigeminal ganglion development and generated a novel transcriptomic dataset that has been curated to illustrate temporally dynamic gene expression patterns. This publicly available resource identifies major pathways involved in trigeminal gangliogenesis, particularly with respect to the condensation and maturation of placode-derived neurons, thus inviting new lines of research into the essential processes governing trigeminal ganglion development.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"520 ","pages":"Pages 108-116"},"PeriodicalIF":2.5,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analysis of neural crest development in the chick and mouse 鸡与小鼠神经嵴发育的比较分析。

IF 2.5 3区生物学

Developmental biology Pub Date : 2024-12-21 DOI: 10.1016/j.ydbio.2024.12.014

J.A. Morrison , I. Pushel , R. McLennan , M.C. McKinney , M.M. Gogol , A. Scott , R. Krumlauf , P.M. Kulesa

{"title":"Comparative analysis of neural crest development in the chick and mouse","authors":"J.A. Morrison , I. Pushel , R. McLennan , M.C. McKinney , M.M. Gogol , A. Scott , R. Krumlauf , P.M. Kulesa","doi":"10.1016/j.ydbio.2024.12.014","DOIUrl":"10.1016/j.ydbio.2024.12.014","url":null,"abstract":"<div><div>A core framework of the gene regulatory network (GRN) governing neural crest (NC) cell development has been generated by integrating separate inputs from diverse model organisms rather than direct comparison. This has limited insights into the diversity of genes in the NC cell GRN and extent of conservation of newly identified transcriptional signatures in cell differentiation and invasion. Here, we address this by leveraging the strengths and accessibility of the avian embryo to precise developmental staging by egg incubation and use an integrated analysis of chick (HH13) and mouse (E9.5) embryo tissue samples collected during NC cell migration into pharyngeal arches 1–2 (PA1 and PA2). We successfully identify a cluster of NC cells containing both mouse and chick cells that share expression of <em>Lmo4</em>, <em>Tfap2B</em>, <em>Sox10</em>, and <em>Twist1</em>, and distinct genes that lack known conserved roles in NC. Importantly, we discovered a cluster of cells exhibiting a conserved transcriptional signature associated with the NC cell migratory wavefront in both mouse and chick, including KAZALD1, BAMBI, DES, and GPC3. We confirm their expression is restricted to leader mouse NCs by multiplexed FISH. Together, these data offer novel insights into the transcriptional programs that underlie NC cell migration and establish the foundation for future comparative functional analyses.</div></div>","PeriodicalId":11070,"journal":{"name":"Developmental biology","volume":"519 ","pages":"Pages 142-149"},"PeriodicalIF":2.5,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142880679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0