Cryo letters最新文献

Effect of Hericium erinaceus polysaccharides on the cryopreservation of bull semen.

IF 1 4区生物学

Cryo letters Pub Date : 2025-03-01

M I Sergushkina, T V Polezhaeva, O N Solomina, A N Khudyakov, O O Zaitseva, Y I Nazarova

{"title":"Effect of Hericium erinaceus polysaccharides on the cryopreservation of bull semen.","authors":"M I Sergushkina, T V Polezhaeva, O N Solomina, A N Khudyakov, O O Zaitseva, Y I Nazarova","doi":"","DOIUrl":"","url":null,"abstract":"Background: The field of sperm cryopreservation requires the search for and development of new effective cryopreservatives with low toxicity.Objective: The purpose of this study was to evaluate the characteristics of bull spermatozoa subjected to freezing and storage at -80 degree C for 28 days in a diluent with the addition of Hericium erinaceus polysaccharides.Materials and methods: The sperm was frozen under the protection of a complex cryoprotectant and stored in an electric freezer at -80 degree C for 28 days. Before cooling and after defrosting, the biological parameters of the sperm were analyzed using the Argus-CASA system.Results: It was established that two fractions isolated from H. erinaceus (PF1, PF2) are heteropolysaccharide peptides with a high content of glucose, xylose, mannose, galactose and arabinose. It was shown that before cooling, when a fraction obtained from dry fruiting bodies of H. erinaceus (PF2) was added to the sperm, the number of progressively motile sperm with intact DNA decreased, but their kinematic parameters did not change. After thawing, after 28 days of storage at -80 degree C, an increase in the number of progressively motile sperm with intact DNA and their kinematic parameters was observed.Conclusion: Thus, fungal polysaccharides are obviously underestimated macromolecular substances with enormous cryostabilization potential, and polysaccharide fractions from dried fruiting bodies of H. erinaceus may in the future find wide application in the composition of new cryopreservation media. https://doi.org/10.54680/fr25210110912.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"46 2","pages":"98-107"},"PeriodicalIF":1.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Challenges in bird cryopreservation.

IF 1 4区生物学

Cryo letters Pub Date : 2025-03-01

J Perez-Rivero

{"title":"Challenges in bird cryopreservation.","authors":"J Perez-Rivero","doi":"","DOIUrl":"","url":null,"abstract":"Cryopreservation is a fundamental technique for preserving the structural and functional integrity of biological material, particularly for the conservation of genetic resources in avian species. Since its development in the 1940s, this technology has advanced significantly, although challenges persist, primarily due to the unique morphology of avian sperm, which complicates cryoprotectant penetration and increases the risk of structural damage. Overcoming these challenges is crucial for improving semen preservation, supporting the sustainability of avian species, and contributing to conservation efforts. In domestic production birds, cryopreservation is essential for maintaining genetic diversity. However, these species often exhibit low tolerance to the freezing process, primarily due to the high concentration of polyunsaturated fatty acids in their sperm membranes, making them susceptible to oxidative damage. This has driven research aimed at developing more effective cryoprotectants and techniques to enhance semen quality post-thaw. Wild birds, particularly endangered species, face additional challenges in cryopreservation. These species are often managed in captivity to prevent extinction, with artificial insemination serving as a valuable tool. However, artificial insemination is constrained by low post-thaw motility rates, even when advanced cryoprotectants are employed. Research indicates that certain cryopreservation media can improve sperm motility and fertility rates, although further optimization of these methods is required. The future of avian semen cryopreservation will concentrate on customizing extenders and cryoprotectants, optimizing freezing techniques, and improving post-thaw semen quality. These advancements are essential for enhancing commercial poultry production and for the conservation of endangered species. Research in this area is expected to evolve over the next decade, developing effective solutions to address both commercial and conservation needs. https://doi.org/10.54680/fr25210110312.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"46 2","pages":"74-81"},"PeriodicalIF":1.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A ten-year retrospective on the efficacy of droplet vitrification for cryobanking of Allium ramosum L. germplasm.

IF 1 4区生物学

Cryo letters Pub Date : 2025-03-01

S Chander, R Gowthami, R Pandey, D A Deepak, A Agrawal

{"title":"A ten-year retrospective on the efficacy of droplet vitrification for cryobanking of Allium ramosum L. germplasm.","authors":"S Chander, R Gowthami, R Pandey, D A Deepak, A Agrawal","doi":"","DOIUrl":"","url":null,"abstract":"Background: Allium ramosum is an important member of the genus Allium, which is commonly known as Chinese chive or fragrant-flowered garlic. Conserving the genetic diversity of different species of Allium is crucial, and cryopreservation has emerged as an important strategy for long-term conservation of alliums.Objective: To develop a reliable protocol for the cryoconservation of A. ramosum shoot bases.Materials and methods: Different parameters, viz. (a) cold-hardening (5 degree C for 16/8 h photoperiod), (b) PVS2 dehydration (0, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55 and 60 min), (c) pregrowth medium (MM3: MS + 0.1 mg/L NAA + 0.5 mg/L 2iP + 10 mg/L spermidine + 3% sucrose; MM10: MS + 0.1 mg/L NAA + 0.5 mg/l 2iP + 10 mg/L spermidine + 10% sucrose) and (d) preculture duration (1, 2, 3, 4 and 5 days) were tested using a vitrification technique.Results: Shoot bases excised from 4-wk old in vitro cultures that had been cold-hardened at 5 degree C (16/8 h photoperiod) and precultured on MM10 with 10% sucrose at 5 degree C for 3 days resulted in highest post-thaw regrowth of 43% after conventional vitrification. However, when droplet-vitrification was used, post-thaw regrowth was increased to 77%. Retesting of shoot bases after 10 years of cryobanking, revealed no significant difference in the post-thaw regrowth of A. ramosum.Conclusion: This is the first report of the long-term cryopreservation of A. ramosum shoot bases using vitrification and droplet-vitrification techniques. https://doi.org/10.54680/fr25210110512.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"46 2","pages":"82-91"},"PeriodicalIF":1.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exposure of carrot seeds to cryopreservation increases root weight and decreases levels of cell wall-linked phenolics.

IF 1 4区生物学

Cryo letters Pub Date : 2025-03-01

Y Entensa, G Lorente, L Perez-Bonachea, J R Ynchausti, J Martinez, B E Zevallos-Bravo, B Companioni, E Hajari, Y Acosta, H W Pritchard, J C Lorenzo

{"title":"Exposure of carrot seeds to cryopreservation increases root weight and decreases levels of cell wall-linked phenolics.","authors":"Y Entensa, G Lorente, L Perez-Bonachea, J R Ynchausti, J Martinez, B E Zevallos-Bravo, B Companioni, E Hajari, Y Acosta, H W Pritchard, J C Lorenzo","doi":"","DOIUrl":"","url":null,"abstract":"Background: The long-term preservation of plant germplasm is critical to ensure a pool of genetic diversity for future breeding efforts and for conservation management.Although it is important to assess whether the biochemical properties and vigour of the germplasm remain unchanged.Objective: To investigate the potential effects of cryopreservation in liquid nitrogen (LN) as a strategy for conservation of carrot.Results: Seeds were exposed to cryogenic temperatures and growth and development was monitored in Petri dishes (for up to 14 days) and in a pot trial (90 days). By day 7, 53% control seeds had germinated compared with 70% seeds exposed to LN. Biochemical attributes were also assessed. Initial differences were observed in the growth rate of plants, where plants originating from seeds exposed to LN emerged faster than those originating from control seeds. However, at the end of the pot trial, differences were only observed in belowground biomass (i.e., mass of carrots). Biochemical evaluations showed that carotenoids and cell wall-linked phenolics were elevated in carrots produced from seeds exposed to LN.Conclusion: Overall, the results show that cryopreservation can be used as a viable strategy for long-term preservation of carrot germplasm. https://doi.org/10.54680/fr25210110812.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"46 2","pages":"135-142"},"PeriodicalIF":1.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrastructure assessment of oocyte from mouse with polycystic ovary syndrome following cryopreservation.

IF 1 4区生物学

Cryo letters Pub Date : 2025-03-01

S Afzalnia, F Ghasemian, H S Maryan, T Mirzapour

{"title":"Ultrastructure assessment of oocyte from mouse with polycystic ovary syndrome following cryopreservation.","authors":"S Afzalnia, F Ghasemian, H S Maryan, T Mirzapour","doi":"","DOIUrl":"","url":null,"abstract":"Background: The polycystic ovary syndrome (PCOS) is a substantial obstacle to female fertility due to ovulation inhibition. Oocyte cryopreservation is crucial for preserving fertility in women with fertility-compromising disorders such as PCOS.Objective: In this study, the ultrastructural damages of oocytes were evaluated following freezing from PCOS mouse model.Materials and methods: This experimental study was conducted on 30 adult NMRI mouse. The study comprised three groups: 1) unfrozen PCOS oocytes; 2) vitrified-thawed control oocytes; and 3) vitrified-thawed PCOS oocytes. Transmission electron microscopy was employed for ultrastructure examination across all groups. Moreover, the expression of apoptotic genes, including BAX and Bcl2, was assessed using real time-quantitative polymerase chain reaction (RT-PCR).Results: The oocyte cryopreservation process had a high impact on the destruction of ooplasm cortical granules, Golgi complexes and mitochondria in vitrified-thawed PCOS oocytes compared to the other groups. In PCOS oocytes, particularly those that were vitrified-thawed, there was a notable increase in vacuolation, with a higher abundance of larger and more numerous vacuoles observed compared to the control group. The vitrified-thawed PCOS group also exhibited a notable increase in the expression of the apoptotic gene compared to the other groups (p < 0.05).Conclusion: A precise evaluation of oocyte cryopreservation is imperative for improving this technique and for producing high-quality oocytes with enhanced fertility potential. This study contributes valuable insights into understanding the intricate relationship between PCOS, cryopreservation and oocyte quality. https://doi.org/10.54680/fr25210110412.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"46 2","pages":"116-125"},"PeriodicalIF":1.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Verification of the decrease in cell recovery after freezing and thawing due to suboptimal shipping using nine cancer cell lines and the differences in impacts between the cell lines.

IF 1 4区生物学

Cryo letters Pub Date : 2025-03-01

M Sato, Y Nakata, M Noguchi, S Araki, Y Satsuo

{"title":"Verification of the decrease in cell recovery after freezing and thawing due to suboptimal shipping using nine cancer cell lines and the differences in impacts between the cell lines.","authors":"M Sato, Y Nakata, M Noguchi, S Araki, Y Satsuo","doi":"","DOIUrl":"","url":null,"abstract":"Background: The impacts of suboptimal shipping conditions during transport on cell viability, recovery, and function of cryopreserved samples, have not been well studied.Objective: The impacts of suboptimal shipping on viability and recovery after the freezing and thawing were investigated using nine cancer cell lines, with particular reference to the approximate level of exposure temperature and exposure time at which adverse effects occur, and whether there are differences in sensitivity between cell types.Materials and methods: The adverse effects of any set of suboptimal shipping conditions (-80 degree C for 7 d, -65 degree C or -50 degree C for 1, 3, and 7 d) on nine cancer cell lines (CHO-K1, COS-1, HeLa, HepG2, HL-60, Jurkat, MCF7, MDCK, 293T) were compared with data obtained during storage in liquid nitrogen.Results: No statistically significant decrease in viability was observed in seven of the nine cell lines after freezing and thawing. On the other hand, a statistically significant decrease in the cell recovery was observed after 2 d post freezing and thawing in the nine cell lines, except CHO-K1 at higher exposure temperatures and longer exposure times. Visualization of the adverse effects on the cell lines using a heat map showed that the impacts tended to be more pronounced under the condition of exposure at -50 degree C for three or more days.Conclusion: These results will contribute to the development of standardized protocols and best practices for the optimal shipping of frozen animal cells. https://doi.org/10.54680/fr25210110212.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"46 2","pages":"108-115"},"PeriodicalIF":1.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fortification of semen extender with mifepristone improves the cryo-survival of cattle spermatozoa.

IF 1 4区生物学

Cryo letters Pub Date : 2025-03-01

A Kumar, V Sachan, M Anand, A Kumar, N Chaudhary, G Singh, M Kumar, J K Agrawal, A Saxena

{"title":"Fortification of semen extender with mifepristone improves the cryo-survival of cattle spermatozoa.","authors":"A Kumar, V Sachan, M Anand, A Kumar, N Chaudhary, G Singh, M Kumar, J K Agrawal, A Saxena","doi":"","DOIUrl":"","url":null,"abstract":"Background: Progesterone, which is present in the semen extender as a component of egg yolk is a potential inducer of capacitation in spermatozoa during cryopreservation. An anti-progesterone component in the extender may protect the spermatozoa from being capacitated and pre-acrosome reacted during cryopreservation. It may lead to better quality of post-thaw sperm population for improved conception.Objective: To investigate the effect of mifepristone on the cryo-survivability of cattle spermatozoa.Materials and methods: Thirty two semen ejaculates were collected from four Sahiwal bulls and divided into three fractions. These fractions were extended with egg yolk-based TRIS extender supplemented with different concentrations of mifepristone (0, 10 and 20 uM) and subjected to cryopreservation. Cryopreserved semen samples were thawed and evaluated for spermatozoa motion parameters (CASA), viability (flow cytometer), hypo-osmotic swelling test (HOST) responsiveness, capacitation status (CTC), acrosome reaction (flow cytometer) and intracellular calcium ion concentrations (flow cytometer).Results: There was no definitive effect of mifepristone on sperm motility and kinematics. However, the semen samples which were treated with mifepristone showed significantly higher spermatozoa viability and HOST responsiveness. Mifepristone also protected spermatozoa from being cryo-capacitated during the preservation process. Higher percentages of uncapacitated and acrosome intact spermatozoa were found at the post-thaw stage in comparison to the untreated group. Mifepristone-treated groups showed fewer spermatozoa with high intracellular calcium levels.Conclusion: A 10 uM concentration of mifepristone has better potential to protect the spermatozoa from progesterone-induced cryo-capacitation and premature acrosome reaction during cryopreservation. https://doi.org/10.54680/fr25210110612.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"46 2","pages":"126-134"},"PeriodicalIF":1.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strategies used by organisms to survive very cold climates - student's guide.

IF 1 4区生物学

Cryo letters Pub Date : 2025-03-01

S P Chesterfield, H V Joyce, T D Fooks, P W Wilson

引用次数: 0

Formation of an outer glassy shell hinders the drying of trehalose droplets.

IF 1 4区生物学

Cryo letters Pub Date : 2025-03-01

G Pei, Y Zhang, P Zhou, C Gao

{"title":"Formation of an outer glassy shell hinders the drying of trehalose droplets.","authors":"G Pei, Y Zhang, P Zhou, C Gao","doi":"","DOIUrl":"","url":null,"abstract":"Background: Airflow drying of mammalian cells has not been successful yet, with one obstacle being the improper drying time of cells-laden trehalose droplets.Objective: To evaluate the major factors affecting the drying kinetics of aqueous trehalose droplets.Materials and methods: A numerical analysis was applied to the evaporation behavior of aqueous trehalose droplets for the preservation of biomaterials. Factors such as convection caused by droplet contraction, vapor diffusion, and Stefan flow around droplets were taken into account in the analysis.Results: Reducing the size and initial concentration of the droplets helps to achieve rapid drying of droplets. Forced convection can effectively enhance the initial drying rate of droplets, which may mitigate cell damage caused by hypertonic solutions. Upon drying, however, the rapid formation of an outer glassy shell inhibits the further drying of trehalose droplets.Conclusion: Simple enhancement of convection does not facilitate complete droplet vitrification due to the formation of an outer glassy shell that prevents water movement. https://doi.org/10.54680/fr25210110712.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"46 2","pages":"92-97"},"PeriodicalIF":1.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production and cryopreservation of 3d cultures.

IF 1 4区生物学

Cryo letters Pub Date : 2025-01-01

N Moisieieva, O Gorina, A Moisieiev, O Prokopiuk

引用次数: 0