{"title":"H2S信号增强了含水芸苔种子的贮藏球蛋白水解、胚过冷性和抗冻性。","authors":"Y Han, J Wang, L Bo, D Song, W Li, B Liu","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Seed storage globulins have two subunits linked by disulfide bonds. Earlier studies suggested that globulin hydrolysis may enhance the freezing tolerance of hydrated seeds. As a donor for H<sub>2</sub>S, NaHS can act as a nucleophile to break the disulfide bond of proteins and convert sulfhydryl group (-SH) of cysteine to the persulfide group (-SSH), which will promote formation of S-persulfidation and de-polymerization of seed globulins.</p><p><strong>Objective: </strong>To examine the role of the H<sub>2</sub>S signal pathway in the freezing tolerance of hydrated brassica seeds.</p><p><strong>Materials and methods: </strong>Hydrated brassica (Brassica oleracea) seeds were treated with 5 mM NaHS to reduce the disulfide bonds of seed globulins and its effect on seed freezing tolerance was investigated.</p><p><strong>Results: </strong>NaHS treatment increased embryo supercooling as determined by differential scanning calorimetry (DSC) and seed survival upon slow cooling (control vs NaHS, 30.0% vs 45.3%). NaHS treatment resulted in a significant increase of sulfhydryl groups of storage globulin, indicating the reduction of disulfide bonds. The 2D electrophoresis showed the depolymerization of storage globulins and the accumulation of small polypeptides. In addition, NaHS treatment increased the contents of ascorbate and glutathione for anti-oxidation.</p><p><strong>Conclusion: </strong>The H<sub>2</sub>S signal pathway is likely involved in the freezing tolerance of hydrated brassica seeds via the de-polymerization and hydrolysis of seed storage globulins, as well as the regulation of supercooling. Doi.org/10.54680/fr25310110312.</p>","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"46 3","pages":"164-171"},"PeriodicalIF":1.0000,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"H<sub>2</sub>S signal enhances storage globulin hydrolysis, embryo supercooling and freezing tolerance of hydrated brassica (Brassica oleracea) seeds.\",\"authors\":\"Y Han, J Wang, L Bo, D Song, W Li, B Liu\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Seed storage globulins have two subunits linked by disulfide bonds. Earlier studies suggested that globulin hydrolysis may enhance the freezing tolerance of hydrated seeds. As a donor for H<sub>2</sub>S, NaHS can act as a nucleophile to break the disulfide bond of proteins and convert sulfhydryl group (-SH) of cysteine to the persulfide group (-SSH), which will promote formation of S-persulfidation and de-polymerization of seed globulins.</p><p><strong>Objective: </strong>To examine the role of the H<sub>2</sub>S signal pathway in the freezing tolerance of hydrated brassica seeds.</p><p><strong>Materials and methods: </strong>Hydrated brassica (Brassica oleracea) seeds were treated with 5 mM NaHS to reduce the disulfide bonds of seed globulins and its effect on seed freezing tolerance was investigated.</p><p><strong>Results: </strong>NaHS treatment increased embryo supercooling as determined by differential scanning calorimetry (DSC) and seed survival upon slow cooling (control vs NaHS, 30.0% vs 45.3%). NaHS treatment resulted in a significant increase of sulfhydryl groups of storage globulin, indicating the reduction of disulfide bonds. The 2D electrophoresis showed the depolymerization of storage globulins and the accumulation of small polypeptides. In addition, NaHS treatment increased the contents of ascorbate and glutathione for anti-oxidation.</p><p><strong>Conclusion: </strong>The H<sub>2</sub>S signal pathway is likely involved in the freezing tolerance of hydrated brassica seeds via the de-polymerization and hydrolysis of seed storage globulins, as well as the regulation of supercooling. Doi.org/10.54680/fr25310110312.</p>\",\"PeriodicalId\":10937,\"journal\":{\"name\":\"Cryo letters\",\"volume\":\"46 3\",\"pages\":\"164-171\"},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2025-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Cryo letters\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cryo letters","FirstCategoryId":"99","ListUrlMain":"","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
背景：种子储存球蛋白有两个亚基由二硫键连接。早期的研究表明，球蛋白水解可以提高水合种子的抗冻性。NaHS作为H2S的供体，可以作为亲核试剂破坏蛋白质的二硫键，将半胱氨酸的巯基（-SH）转化为过硫基（-SSH），促进种子球蛋白s -过硫的形成和解聚。目的：探讨H2S信号通路在含水芸苔种子抗冻性中的作用。材料与方法：采用5 mM NaHS处理水合的芸苔种子，降低种子球蛋白的二硫键，研究其对种子抗冻性的影响。结果：通过差示扫描量热法（DSC）测定，NaHS处理增加了胚胎过冷度和缓慢冷却时的种子存活率（对照与NaHS， 30.0% vs 45.3%）。NaHS处理导致储存球蛋白的巯基显著增加，表明二硫键减少。二维电泳显示储存球蛋白解聚和小多肽积累。此外，NaHS处理增加了抗坏血酸和谷胱甘肽的含量，具有抗氧化作用。结论：H2S信号通路可能通过储种球蛋白的解聚、水解及过冷调控参与了含水芸苔种子的抗冻性。Doi.org/10.54680/fr25310110312。
H2S signal enhances storage globulin hydrolysis, embryo supercooling and freezing tolerance of hydrated brassica (Brassica oleracea) seeds.
Background: Seed storage globulins have two subunits linked by disulfide bonds. Earlier studies suggested that globulin hydrolysis may enhance the freezing tolerance of hydrated seeds. As a donor for H2S, NaHS can act as a nucleophile to break the disulfide bond of proteins and convert sulfhydryl group (-SH) of cysteine to the persulfide group (-SSH), which will promote formation of S-persulfidation and de-polymerization of seed globulins.
Objective: To examine the role of the H2S signal pathway in the freezing tolerance of hydrated brassica seeds.
Materials and methods: Hydrated brassica (Brassica oleracea) seeds were treated with 5 mM NaHS to reduce the disulfide bonds of seed globulins and its effect on seed freezing tolerance was investigated.
Results: NaHS treatment increased embryo supercooling as determined by differential scanning calorimetry (DSC) and seed survival upon slow cooling (control vs NaHS, 30.0% vs 45.3%). NaHS treatment resulted in a significant increase of sulfhydryl groups of storage globulin, indicating the reduction of disulfide bonds. The 2D electrophoresis showed the depolymerization of storage globulins and the accumulation of small polypeptides. In addition, NaHS treatment increased the contents of ascorbate and glutathione for anti-oxidation.
Conclusion: The H2S signal pathway is likely involved in the freezing tolerance of hydrated brassica seeds via the de-polymerization and hydrolysis of seed storage globulins, as well as the regulation of supercooling. Doi.org/10.54680/fr25310110312.
期刊介绍：
A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
