Endoplasmic reticulum stress genes and apoptosis-related genes effect cryotolerance: Two comparisons of balb/c and cd-1 mice.

IF 1 4区生物学 Q3 BIOLOGY

Cryo letters Pub Date : 2025-07-01

A Eraslan Sakar, O K Yalcin, C Yildiz, F Yilmaz Dayanc

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引用次数: 0

Abstract

Background: The inbred C57BL/6 and BALB/c mouse strains are widely recognized and used as foundational models for mutagenesis studies globally. Analyzing molecular damage at cellular and genetic levels from freeze-thaw processes in different mouse strains is crucial for understanding how to preserve sperm function and reproductive efficiency.

Objective: To examine intraspecific variations in fresh and frozen-thawed sperm from outbred (CD-1) and inbred (BALB/c) mouse strains.

Materials and methods: Sperm cryopreservation utilized 3 % (w/v) skim milk powder and 18 % (w/v) raffinose as cryoprotectants.

Results: Post-thaw analysis showed significantly higher progressive sperm motility (p < 0.05), intact plasma membrane integrity (p < 0.01), and viability (p < 0.05) in CD-1 frozen-thawed sperm than in BALB/c. The mRNA expression of XBP1, GRP78, and IRE1 was significantly higher in BALB/c frozen-thawed sperm (p < 0.001). CHOP mRNA levels showed no significant variation (p > 0.05). BAX mRNA was significantly upregulated in both strains after freezing (p < 0.001). While TCP11 mRNA showed no significant differences (p > 0.05), PDIA3 mRNA increased significantly post-thaw (p < 0.001).

Conclusion: Cryopreservation quality was superior in outbred CD-1 sperm compared to inbred BALB/c sperm, evidenced by better post-thaw parameters and elevated endoplasmic reticulum stress-related genes (XBP1, GRP78, and IRE1) and PDIA3. https://doi.org/10.54680/fr25410110512.

本刊更多论文

内质网应激基因和凋亡相关基因对低温耐受性的影响：balb/c和cd-1小鼠的两种比较。

背景：近交的C57BL/6和BALB/c小鼠品系被广泛认可，并被用作全球诱变研究的基础模型。分析不同小鼠品系冻融过程中细胞和遗传水平的分子损伤对于了解如何保持精子功能和生殖效率至关重要。目的：研究远交系（CD-1）和近交系（BALB/c）小鼠新鲜和冻融精子的种内变异。材料和方法：精子冷冻保存采用3% （w/v）脱脂奶粉和18% （w/v）棉子糖作为冷冻保护剂。结果：解冻后分析显示，CD-1冻融精子的进展精子活力（p < 0.05）、完整的质膜完整性（p < 0.01）和活力（p < 0.05）明显高于BALB/c冻融精子。BALB/c冻融精子中XBP1、GRP78和IRE1 mRNA表达量显著升高（p < 0.001）。CHOP mRNA水平差异无统计学意义（p < 0.05）。两种菌株冷冻后BAX mRNA表达均显著上调（p < 0.001）。TCP11 mRNA在解冻后无显著差异（p < 0.05）， PDIA3 mRNA在解冻后显著升高（p < 0.001）。结论：与近交系BALB/c精子相比，CD-1精子的低温保存质量更好，解冻后参数更好，内质网应激相关基因（XBP1、GRP78和IRE1）和PDIA3升高。https://doi.org/10.54680/fr25410110512。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.