J Bai, G Zhou, J Li, Y Liu, J Wang, Y Guo, Z Zhang, W Liu, A Liu, W Q Sun, P Wan, X Fu
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引用次数: 0
Abstract
Background: Lipids play a vital role in sperm capacity acquisition, and yet the effect of cryopreservation on lipid profile has received little attention so far.
Objective: To evaluate the change in lipids of ovine sperm upon cryopreservation.
Materials and methods: Ovine semen was aliquoted into two parts: one being the fresh control and the other used for cryopreservation. The targeted lipidomic analysis was performed between fresh and cryopreserved sperm samples.
Results: Cryopreservation resulted in 53 up-regulated and 163 down-regulated lipids. Down-regulation prevails. Most differentiated lipids were glycerophospholipid, fatty acyl, glycerolipid, sphingolipid, prenol lipid and sterol, and they were highly correlated with one another (correlation coefficient r > 0.8). Major pathway enrichments were glycerophospholipid metabolism, glycosylphosphatidylinositol-anchor biosynthesis, glycine, serine and threonine metabolism, biosynthesis of unsaturated fatty acids, actin cytoskeleton regulation, pathogenic infection and autophagy. In glycerophospholipid metabolism, the phosphatidyl-ethanolamine Class II series and phosphatidyl-L-serine Class II series had the largest numbers of differentially-expressed lipids, some significantly down-regulated and others up-regulated. In contrast, all lipids of the 1,2-diacyl-sn-glycerol-3P Class II series were significantly down-regulated.
Conclusion: Cryopreservation altered the sperm lipid profile, and the changes in 1,2-diacyl-sn-glycerol-3P, phosphatidyl-ethanolamine and phosphatidyl-L-serine can be good biomarkers for sperm quality change. https://doi.org/10.54680/fr25410110712.
期刊介绍：
A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.
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