The presence of vitamin c enhances the protection of cholesterol-cyclodextrin and vitamin e-cyclodextrin in cryopreservation of bull semen.

IF 1 4区生物学 Q3 BIOLOGY

Cryo letters Pub Date : 2025-07-01

A Khellouf, A Benberkane, L Taouzinet, S Fatmi, M Iguer-Ouada

{"title":"The presence of vitamin c enhances the protection of cholesterol-cyclodextrin and vitamin e-cyclodextrin in cryopreservation of bull semen.","authors":"A Khellouf, A Benberkane, L Taouzinet, S Fatmi, M Iguer-Ouada","doi":"","DOIUrl":null,"url":null,"abstract":"Background: Cryopreservation of sperm cells is a well-established technique. However, cryopreserved sperm cells can have significant cellular damage, primarily due to oxidative stress.Objective: To investigate the synergistic effects of vitamin C with vitamin E-loaded cyclodextrins on the quality of cryopreserved bull sperm cells in combination with cholesterol-loaded cyclodextrins.Materials and methods: The ejaculates from nine mature bulls were divided into six equal aliquots for different treatments, including vitamin C (VitC), vitamin E loaded cyclodextrin (CD-VitE), CD-VitE+VitC, cholesterol loaded cyclodextrin (CD-CHL), CD-CHL + CD-VitE + VitC and the control. The sperm motility (CASA), membrane integrity (HOST) and lipid peroxidation (TBARS) were assessed after freezing and thawing.Results: The combination of CD-CHL + CD-VitE + VitC retained the highest sperm motility and membrane integrity, as well as resulted in the least lipid peroxidation.Conclusion: The study shows the synergistic effects of vitamin C, vitamin E and cholesterol in improving the cryopreservation outcomes of bull sperm. https://doi.org/10.54680/fr25410110312.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"46 4","pages":"231-237"},"PeriodicalIF":1.0000,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cryo letters","FirstCategoryId":"99","ListUrlMain":"","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Cryopreservation of sperm cells is a well-established technique. However, cryopreserved sperm cells can have significant cellular damage, primarily due to oxidative stress.

Objective: To investigate the synergistic effects of vitamin C with vitamin E-loaded cyclodextrins on the quality of cryopreserved bull sperm cells in combination with cholesterol-loaded cyclodextrins.

Materials and methods: The ejaculates from nine mature bulls were divided into six equal aliquots for different treatments, including vitamin C (VitC), vitamin E loaded cyclodextrin (CD-VitE), CD-VitE+VitC, cholesterol loaded cyclodextrin (CD-CHL), CD-CHL + CD-VitE + VitC and the control. The sperm motility (CASA), membrane integrity (HOST) and lipid peroxidation (TBARS) were assessed after freezing and thawing.

Results: The combination of CD-CHL + CD-VitE + VitC retained the highest sperm motility and membrane integrity, as well as resulted in the least lipid peroxidation.

Conclusion: The study shows the synergistic effects of vitamin C, vitamin E and cholesterol in improving the cryopreservation outcomes of bull sperm. https://doi.org/10.54680/fr25410110312.

本刊更多论文

维生素c的存在增强了胆固醇-环糊精和维生素e-环糊精对公牛精液冷冻保存的保护作用。

背景：精子冷冻保存是一项成熟的技术。然而，低温保存的精子细胞可能有显著的细胞损伤，主要是由于氧化应激。目的：探讨维生素C与含维生素e的环糊精联合含胆固醇的环糊精对冷冻公牛精子细胞质量的增效作用。材料与方法：将9头成年公牛的射精分成6等份，分别进行维生素C （VitC）、维生素E负载环糊精（CD-VitE）、CD-VitE+VitC、胆固醇负载环糊精（CD-CHL）、CD-CHL + CD-VitE+VitC和对照处理。冷冻和解冻后分别评估精子活力（CASA）、膜完整性（HOST）和脂质过氧化（TBARS）。结果：CD-CHL + CD-VitE + VitC组合保留了最高的精子活力和膜完整性，并且导致了最少的脂质过氧化。结论：维生素C、维生素E和胆固醇具有提高牛精子低温保存效果的协同作用。https://doi.org/10.54680/fr25410110312。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.